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An Adaptive Genetic Algorithm Optimizes Double-Hidden Layer BPNN for Rapid Detection of Moisture Content of Green Tea in Processing 自适应遗传算法优化双隐层 BPNN,用于快速检测绿茶加工过程中的水分含量
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-14 DOI: 10.1155/2024/5876051
Zeling Zhang, Liyuan Deng
Moisture content (MC) plays a crucial role in evaluating the quality of tea processing. However, the current automated production line for green tea heavily relies on manual methods to determine MC, which leads to low productivity and inadequate automation. Therefore, there is an urgent need for a fast, accurate, and convenient MC detection method. In this study, near-infrared spectroscopy (NIRS) data were collected from seven stages of green tea processing and preprocessed using various techniques, such as Savitzky-Golay (SG) and detrend (DT), to reduce spectral noise. Subsequently, feature variables of the preprocessed spectral data were selected using full-band principal component analysis (PCA) and competitive adaptive reweighted sampling (CARS). Afterwards, prediction models for MC of green tea were developed using partial least squares regression (PLSR) and back-propagation neural network (BPNN). To address the convergence speed and local optima issues of BPNN, the study proposes an adaptive probabilistic genetic algorithm (AGA) to optimize the initial weights and thresholds of BPNN, including single and double-hidden layers, respectively. The results demonstrate that the double-hidden SG-DT-PCA-AGA-BPNN model outperforms the single-hidden layer model, achieving a high correlation coefficient (RP) of 0.994 and a low root mean square error (RMSEP) of 1.01%. This study highlights the effectiveness of increasing the number of hidden layers and using AGA to optimize the initial thresholds and weights of BPNN in improving the prediction accuracy. Furthermore, it provides a new approach to implement MC detection technology in green tea processing.
水分含量(MC)在评估茶叶加工质量方面起着至关重要的作用。然而,目前的绿茶自动化生产线主要依靠人工方法来测定 MC,导致生产率低下,自动化程度不高。因此,迫切需要一种快速、准确、方便的 MC 检测方法。本研究从绿茶加工的七个阶段收集了近红外光谱(NIRS)数据,并使用萨维茨基-戈莱(SG)和去趋势(DT)等多种技术进行预处理,以减少光谱噪声。随后,利用全波段主成分分析(PCA)和竞争性自适应再加权采样(CARS)对预处理后的光谱数据进行特征变量筛选。之后,利用偏最小二乘回归(PLSR)和反向传播神经网络(BPNN)建立了绿茶 MC 的预测模型。针对 BPNN 的收敛速度和局部最优问题,研究提出了一种自适应概率遗传算法(AGA)来优化 BPNN 的初始权值和阈值,包括单隐层和双隐层。结果表明,双隐 SG-DT-PCA-AGA-BPNN 模型优于单隐层模型,达到了 0.994 的高相关系数(RP)和 1.01% 的低均方根误差(RMSEP)。这项研究强调了增加隐藏层数和使用 AGA 优化 BPNN 初始阈值和权重在提高预测准确性方面的有效性。此外,它还为在绿茶加工中实施 MC 检测技术提供了一种新方法。
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引用次数: 0
Effect of Fermentation Conditions (Dilution Ratio, Medium pH, Total Soluble Solids, and Saccharomyces cerevisiae Yeast Ratio) on the Ability to Ferment Cider from Tamarillo (Solanum betaceum) Fruit 发酵条件(稀释比例、培养基 pH 值、可溶性固形物总量和酿酒酵母比例)对柽柳果(Solanum betaceum)苹果酒发酵能力的影响
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/8841207
Cao Xuan Thuy, Van Thinh Pham, Trinh Thi Nhu Hang Nguyen, Thi Tuyet Ngan Nguyen, Nu Thuy An Ton, Tran Thi Tuu, Ngoc Duc Vu

Tamarillo (Solanum betaceum) is a nutrition-rich product containing antioxidant components and preventive properties against cancer risk. However, there is currently a scarcity of research on processing techniques to diversify products and extend the preservation time of the active compounds in tamarillo. In this study, we focused on developing a cider processing procedure from tamarillo by fermentation with Saccharomyces cerevisiae (Angel RV002). Fermentation conditions, such as the dilution ratio (66 : 34–34 : 66%, w/w), medium pH (3.5–5), total soluble solids (TSS 10–26°Brix), and yeast ratio (0.6–1.2 g/L) were investigated. Ethanol concentration, pH, TSS, titratable acidity, total sugar content, and reducing sugar content were evaluated from day 0 to day 5 of fermentation. At a 50 : 50 (%, w/w) dilution ratio of the tamarillo juice with water, pH 4.5, TSS of 22°Brix, and the addition of yeast 0.6 g/L to the fermentation process, the ethanol concentration reached 7.54 ± 0.11 (%, v/v) after 4 days of fermentation. Additionally, the product maintained a moderately low pH (pH 4.16). The final product exhibited a high sugar content and dissolved nutrients. The results of this study are expected to serve as a basis for the production of tamarillo cider, contributing to the diversification of the product, enhancing the value of tamarillo, and promoting economic development in the region of cultivation.

柽柳(Solanum betaceum)是一种营养丰富的产品,含有抗氧化成分和预防癌症风险的特性。然而,目前有关使产品多样化并延长柽柳中活性化合物保存时间的加工技术的研究还很少。在这项研究中,我们重点开发了利用酿酒酵母(Angel RV002)进行发酵的苹果酒加工程序。研究了发酵条件,如稀释比例(66 : 34-34 : 66%,w/w)、培养基 pH 值(3.5-5)、总可溶性固形物(TSS 10-26°Brix)和酵母比例(0.6-1.2 克/升)。从发酵的第 0 天到第 5 天,对乙醇浓度、pH 值、TSS、可滴定酸度、总糖含量和还原糖含量进行了评估。在 50 :在罗望子汁与水的稀释比例为 50 : 50(%,w/w)、pH 值为 4.5、TSS 为 22°Brix、发酵过程中添加 0.6 克/升酵母的条件下,发酵 4 天后乙醇浓度达到 7.54±0.11(%,v/v)。此外,产品的 pH 值保持在中等偏低的水平(pH 值为 4.16)。最终产品显示出较高的含糖量和溶解营养成分。这项研究的结果可作为生产罗望子苹果酒的基础,有助于实现产品多样化,提高罗望子的价值,促进种植地区的经济发展。
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引用次数: 0
Chlorpyrifos Pesticide Removal from Black Grapes Using Plasma-Activated Water Produced by Plasma Bubbling Technology 利用等离子体气泡技术产生的等离子体活化水去除黑葡萄中的毒死蜱农药
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/7856706
B. Rakesh, R. Anbarasan, B. Kamalapreetha, R. Purushothaman, B. R. Ashutosh, R. Mahendran
This study is aimed at evaluating the ability of plasma-activated water (PAW) to reduce the chlorpyrifos pesticide residue in black grapes. Raw black grapes were spiked with a known concentration (10, 15, and 20 ppm) of chlorpyrifos (20% EC) and bubbled with 120 V air plasma at three different time intervals (5, 10, and 15 min). GC-MS analysis of these plasma-bubbled grapes showed up to 65.25% reduction (20 ppm sample) in chlorpyrifos content after 15 min of treatment. However, the treatment also reduced the grape juice’s ascorbic acid (19.97±2.69 to 9.15±2.02 mg/ml), antioxidant scavenging activity (77.42±2.97 to 53.30±4.77%), total flavonoids (3.00±0.53 to 2.61±0.57 mg QE/ml), total soluble solids (14.23±1.96 to 11.95±1.86°), total suspended solids (1.95±0.16 to 1.80±0.03 g/l), and turbidity (246.63±11.42 to 224.1±24.85 NTU). Meanwhile, other physicochemical attributes such as pH, titrable acidity, total phenol content, color index, and texture values had slight changes after plasma bubbling. Thus, plasma bubbling proved to be an effective method to remove the chlorpyrifos pesticide present in grapes, and the techniques also preserve the quality of the commodity.
本研究旨在评估等离子体活化水(PAW)减少黑葡萄中毒死蜱农药残留的能力。在未加工的黑葡萄中添加已知浓度(10、15 和 20 ppm)的毒死蜱(20%EC),并以三个不同的时间间隔(5、10 和 15 分钟)用 120 V 空气等离子体鼓泡。等离子气泡葡萄的气相色谱-质谱(GC-MS)分析表明,经过 15 分钟处理后,毒死蜱含量减少了 65.25%(20 ppm 样品)。然而,处理也降低了葡萄汁中的抗坏血酸(19.97±2.69 至 9.15±2.02 mg/ml)、抗氧化清除活性(77.42±2.97 至 53.30±4.77%)、总黄酮(3.00±0.53 至 2.61±0.53)、抗氧化活性(77.42±2.97 至 53.30±4.77%)、抗氧化清除活性(77.42±2.97 至 53.30±4.77%)。53 至 2.61±0.57 毫克 QE/毫升)、总可溶性固形物(14.23±1.96 至 11.95±1.86°)、总悬浮固体(1.95±0.16 至 1.80±0.03 克/升)和浊度(246.63±11.42 至 224.1±24.85 NTU)。同时,其他理化属性如 pH 值、滴定酸度、总酚含量、色度指数和质地值在等离子体鼓泡后也有轻微变化。因此,等离子体气泡法被证明是去除葡萄中毒死蜱农药的有效方法,而且该技术还能保持葡萄的品质。
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引用次数: 0
Chlorpyrifos Pesticide Removal from Black Grapes Using Plasma-Activated Water Produced by Plasma Bubbling Technology 利用等离子体气泡技术产生的等离子体活化水去除黑葡萄中的毒死蜱农药
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/7856706
B. Rakesh, R. Anbarasan, B. Kamalapreetha, R. Purushothaman, B. Ram Ashutosh, R. Mahendran

This study is aimed at evaluating the ability of plasma-activated water (PAW) to reduce the chlorpyrifos pesticide residue in black grapes. Raw black grapes were spiked with a known concentration (10, 15, and 20 ppm) of chlorpyrifos (20% EC) and bubbled with 120 V air plasma at three different time intervals (5, 10, and 15 min). GC-MS analysis of these plasma-bubbled grapes showed up to 65.25% reduction (20 ppm sample) in chlorpyrifos content after 15 min of treatment. However, the treatment also reduced the grape juice’s ascorbic acid (19.97 ± 2.69 to 9.15 ± 2.02 mg/ml), antioxidant scavenging activity (77.42 ± 2.97 to 53.30 ± 4.77%), total flavonoids (3.00 ± 0.53 to 2.61 ± 0.57 mg QE/ml), total soluble solids (14.23 ± 1.96 to 11.95 ± 1.86°), total suspended solids (1.95 ± 0.16 to 1.80 ± 0.03 g/l), and turbidity (246.63 ± 11.42 to 224.1 ± 24.85 NTU). Meanwhile, other physicochemical attributes such as pH, titrable acidity, total phenol content, color index, and texture values had slight changes after plasma bubbling. Thus, plasma bubbling proved to be an effective method to remove the chlorpyrifos pesticide present in grapes, and the techniques also preserve the quality of the commodity.

本研究旨在评估等离子体活化水(PAW)减少黑葡萄中毒死蜱农药残留的能力。在未加工的黑葡萄中添加已知浓度(10、15 和 20 ppm)的毒死蜱(20%EC),并以三个不同的时间间隔(5、10 和 15 分钟)用 120 V 空气等离子体鼓泡。等离子气泡葡萄的气相色谱-质谱(GC-MS)分析表明,经过 15 分钟处理后,毒死蜱含量减少了 65.25%(20 ppm 样品)。然而,处理也降低了葡萄汁中的抗坏血酸(19.97±2.69 至 9.15±2.02 mg/ml)、抗氧化清除活性(77.42±2.97 至 53.30±4.77%)、总黄酮(3.00±0.53 至 2.61±0.53)、抗氧化活性(77.42±2.97 至 53.30±4.77%)、抗氧化清除活性(77.42±2.97 至 53.30±4.77%)。53 至 2.61±0.57 毫克 QE/毫升)、总可溶性固形物(14.23±1.96 至 11.95±1.86°)、总悬浮固体(1.95±0.16 至 1.80±0.03 克/升)和浊度(246.63±11.42 至 224.1±24.85 NTU)。同时,其他理化属性如 pH 值、滴定酸度、总酚含量、色度指数和质地值在等离子体鼓泡后也有轻微变化。因此,等离子体气泡法被证明是去除葡萄中毒死蜱农药的有效方法,而且该技术还能保持葡萄的品质。
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引用次数: 0
The Effect of Whey Protein Concentrate, Soy Protein Isolate, and Xanthan Gum on Textural and Rheological Characteristics of Gluten-Free Batter and Cake 浓缩乳清蛋白、大豆异构蛋白和黄原胶对无麸质面糊和蛋糕质构和流变特性的影响
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/5571107
Parastoo Ghaemi, Saeedeh Arabshahi-Delouee, Mehran Aalami, Seyyed Hossein Hosseini Ghaboos

The market of gluten-free food products has been growing exponentially in recent years. Consequently, it is necessary to conduct research on the effects of various compounds to enhance the quality of these products. The present study is aimed at investigating the effects of adding whey protein concentrate (WPC) and soy protein isolate (SPI) at three levels (0%, 5%, and 10%) and xanthan gum (XG) (0%, 0.15%, and 0.3%) on the textural and rheological characteristics of gluten-free batter and cake based on rice flour. The results showed that increasing the levels of XG, WPC, and SPI improved the apparent viscosity of the batter samples. The effect of WPC on the apparent viscosity was more pronounced than that of SPI, as the highest value (21.9 Pa·s) was related to the sample containing 10% WPC and 0.3% XG. The loss tangent values of the batter samples, measured at a frequency of 1 Hz, ranged from 0.531 to 0.8 for different levels of WPC/XG and from 0.466 to 0.699 for SPI/XG, indicating a gel-like behavior of the batter samples. The sample containing 10% SPI and 0.3% XG displayed the strongest gel with higher storage modulus (982.112 Pa) and loss modulus (458.039 Pa) compared to other samples. Increasing the levels of XG, WPC, and SPI resulted in higher specific volume, porosity, firmness, and springiness of the samples compared to the control sample (P < 0.05). The addition of WPC, SPI, and XG improved the overall acceptability of the cake samples, with the samples containing WPC/XG combination being the most preferred. Ultimately, the cake sample containing 10% WPC along with 0.3% XG introduced as optimal sample. This formulation not only can enhance the characteristics of gluten-free cake but also meets parts of the nutritional needs of consumers, particularly those with celiac disease.

近年来,无麸质食品市场急剧增长。因此,有必要对各种化合物的效果进行研究,以提高这些产品的质量。本研究旨在探讨添加三种浓度(0%、5% 和 10%)的浓缩乳清蛋白(WPC)和大豆分离蛋白(SPI)以及黄原胶(XG)(0%、0.15% 和 0.3%)对以米粉为基础的无麸质面糊和蛋糕的质构和流变特性的影响。结果表明,增加 XG、WPC 和 SPI 的含量可提高面糊样品的表观粘度。WPC 对表观粘度的影响比 SPI 更明显,因为最高值(21.9 Pa-s)与含有 10% WPC 和 0.3% XG 的样品有关。在频率为 1 Hz 时测量的面糊样品损耗正切值,不同水平的 WPC/XG 在 0.531 至 0.8 之间,SPI/XG 在 0.466 至 0.699 之间,表明面糊样品具有凝胶状行为。与其他样品相比,含有 10% SPI 和 0.3% XG 的样品显示出最强的凝胶,具有更高的储存模量(982.112 Pa)和损耗模量(458.039 Pa)。与对照样品相比,增加 XG、WPC 和 SPI 的含量可提高样品的比容、孔隙率、坚固性和回弹性(P<0.05)。添加 WPC、SPI 和 XG 可提高蛋糕样品的整体可接受性,其中最受欢迎的是含有 WPC/XG 组合的样品。最终,含有 10% WPC 和 0.3% XG 的蛋糕样品成为最佳样品。这种配方不仅能增强无麸质蛋糕的特性,还能满足消费者,尤其是乳糜泻患者的部分营养需求。
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引用次数: 0
Thermal Performance and Technoeconomic Analysis of Solar-Assisted Heat Pump Dryer Integrated with Energy Storage Materials for Drying Cavendish Banana (Musa acuminata) 结合储能材料的太阳能辅助热泵干燥机的热性能和技术经济分析,用于干燥卡文迪许香蕉(Musa acuminata)。
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/7496826
A. B. T. Loemba, Baraka Kichonge, T. Kivevele
This study examines a novel solar-assisted heat pump dryer integrated with a thermal energy storage system using soapstone as storage material. The dryer is investigated through experimental analysis across three operating modes: mode 1 with thermal energy storage during daytime, mode 2 without thermal energy storage during nighttime, and mode 3 without thermal energy storage during daytime. Experiments were carried out to investigate the drying of 500 g of Cavendish banana. Thermal performance, as well as economic, and nutritional content were examined. Three replicates of the experiment yielded consistent results, showing a significant reduction in the moisture content of the initial sample from 74.4% to 9.6% after undergoing distinct drying durations. Mode 1 achieved this reduction in 270 minutes, mode 2 in 390 minutes, and mode 3 in 360 minutes. The average specific moisture extraction rates for modes 1, 2, and 3 were 0.13, 0.11, and 0.12 kg/kWh, respectively. Simultaneously, the drying rate ranged from 0.16 to 0.24% per minute. The drying efficiency varied among the tested modes, with mode 1 achieving the highest efficiency at 23.23%. In terms of coefficient of performance, mode 1, mode 2, and mode 3 exhibited values of 3.69, 2.57, and 2.54, respectively. The economic analysis conducted specifically for mode 1 revealed a payback period of 1.5 years, indicating the time required to recover the initial investment. Additionally, the results indicated that the dried Cavendish banana had significantly higher concentrations of proximate parameters and minerals compared to the fresh Cavendish banana, as evidenced by a p value less than 0.05.
本研究探讨了一种新型太阳能辅助热泵干燥机,该干燥机集成了一个热能存储系统,使用皂石作为存储材料。通过对三种运行模式的实验分析,对该干燥机进行了研究:白天带热能储存的模式 1、夜间不带热能储存的模式 2 和白天不带热能储存的模式 3。实验研究了 500 克卡文迪许香蕉的干燥情况。对热性能、经济性和营养成分进行了检验。三次重复实验的结果一致,表明在经过不同的干燥时间后,初始样品的水分含量从 74.4% 显著降低到 9.6%。模式 1 在 270 分钟内实现了水分含量的降低,模式 2 在 390 分钟内实现了水分含量的降低,模式 3 在 360 分钟内实现了水分含量的降低。模式 1、2 和 3 的平均特定抽湿率分别为 0.13、0.11 和 0.12 千克/千瓦时。同时,干燥速率为每分钟 0.16% 至 0.24%。不同测试模式的干燥效率各不相同,其中模式 1 的效率最高,为 23.23%。就性能系数而言,模式 1、模式 2 和模式 3 的数值分别为 3.69、2.57 和 2.54。专门针对模式 1 进行的经济分析表明,投资回收期为 1.5 年,表明收回初始投资所需的时间。此外,结果表明,与新鲜卡文迪许香蕉相比,干卡文迪许香蕉的近似参数和矿物质浓度明显更高,P 值小于 0.05。
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引用次数: 0
Thermal Performance and Technoeconomic Analysis of Solar-Assisted Heat Pump Dryer Integrated with Energy Storage Materials for Drying Cavendish Banana (Musa acuminata) 结合储能材料的太阳能辅助热泵干燥机的热性能和技术经济分析,用于干燥卡文迪许香蕉(Musa acuminata)。
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/7496826
Aldé Belgard Tchicaya Loemba, Baraka Kichonge, Thomas Kivevele

This study examines a novel solar-assisted heat pump dryer integrated with a thermal energy storage system using soapstone as storage material. The dryer is investigated through experimental analysis across three operating modes: mode 1 with thermal energy storage during daytime, mode 2 without thermal energy storage during nighttime, and mode 3 without thermal energy storage during daytime. Experiments were carried out to investigate the drying of 500 g of Cavendish banana. Thermal performance, as well as economic, and nutritional content were examined. Three replicates of the experiment yielded consistent results, showing a significant reduction in the moisture content of the initial sample from 74.4% to 9.6% after undergoing distinct drying durations. Mode 1 achieved this reduction in 270 minutes, mode 2 in 390 minutes, and mode 3 in 360 minutes. The average specific moisture extraction rates for modes 1, 2, and 3 were 0.13, 0.11, and 0.12 kg/kWh, respectively. Simultaneously, the drying rate ranged from 0.16 to 0.24% per minute. The drying efficiency varied among the tested modes, with mode 1 achieving the highest efficiency at 23.23%. In terms of coefficient of performance, mode 1, mode 2, and mode 3 exhibited values of 3.69, 2.57, and 2.54, respectively. The economic analysis conducted specifically for mode 1 revealed a payback period of 1.5 years, indicating the time required to recover the initial investment. Additionally, the results indicated that the dried Cavendish banana had significantly higher concentrations of proximate parameters and minerals compared to the fresh Cavendish banana, as evidenced by a p value less than 0.05.

本研究探讨了一种新型太阳能辅助热泵干燥机,该干燥机集成了一个热能存储系统,使用皂石作为存储材料。通过对三种运行模式的实验分析,对该干燥机进行了研究:白天带热能储存的模式 1、夜间不带热能储存的模式 2 和白天不带热能储存的模式 3。实验研究了 500 克卡文迪许香蕉的干燥情况。对热性能、经济性和营养成分进行了检验。三次重复实验的结果一致,表明在经过不同的干燥时间后,初始样品的水分含量从 74.4% 显著降低到 9.6%。模式 1 在 270 分钟内实现了水分含量的降低,模式 2 在 390 分钟内实现了水分含量的降低,模式 3 在 360 分钟内实现了水分含量的降低。模式 1、2 和 3 的平均特定抽湿率分别为 0.13、0.11 和 0.12 千克/千瓦时。同时,干燥速率为每分钟 0.16% 至 0.24%。不同测试模式的干燥效率各不相同,其中模式 1 的效率最高,为 23.23%。就性能系数而言,模式 1、模式 2 和模式 3 的数值分别为 3.69、2.57 和 2.54。专门针对模式 1 进行的经济分析表明,投资回收期为 1.5 年,表明收回初始投资所需的时间。此外,结果表明,与新鲜卡文迪许香蕉相比,干卡文迪许香蕉的近似参数和矿物质浓度明显更高,P 值小于 0.05。
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引用次数: 0
Effect of Fermentation Conditions (Dilution Ratio, Medium pH, Total Soluble Solids, and Saccharomyces cerevisiae Yeast Ratio) on the Ability to Ferment Cider from Tamarillo (Solanum betaceum) Fruit 发酵条件(稀释比例、培养基 pH 值、可溶性固形物总量和酿酒酵母比例)对柽柳果(Solanum betaceum)苹果酒发酵能力的影响
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-02-02 DOI: 10.1155/2024/8841207
Cao Xuan Thuy, Van Thinh Pham, Trinh Thi Nhu Hang Nguyen, Thi Tuyet Ngan Nguyen, Nu Thuy An Ton, Tran Thi Tuu, Ngoc Duc Vu
Tamarillo (Solanum betaceum) is a nutrition-rich product containing antioxidant components and preventive properties against cancer risk. However, there is currently a scarcity of research on processing techniques to diversify products and extend the preservation time of the active compounds in tamarillo. In this study, we focused on developing a cider processing procedure from tamarillo by fermentation with Saccharomyces cerevisiae (Angel RV002). Fermentation conditions, such as the dilution ratio (66 : 34–34 : 66%, w/w), medium pH (3.5–5), total soluble solids (TSS 10–26°Brix), and yeast ratio (0.6–1.2 g/L) were investigated. Ethanol concentration, pH, TSS, titratable acidity, total sugar content, and reducing sugar content were evaluated from day 0 to day 5 of fermentation. At a 50 : 50 (%, w/w) dilution ratio of the tamarillo juice with water, pH 4.5, TSS of 22°Brix, and the addition of yeast 0.6 g/L to the fermentation process, the ethanol concentration reached 7.54±0.11 (%, v/v) after 4 days of fermentation. Additionally, the product maintained a moderately low pH (pH 4.16). The final product exhibited a high sugar content and dissolved nutrients. The results of this study are expected to serve as a basis for the production of tamarillo cider, contributing to the diversification of the product, enhancing the value of tamarillo, and promoting economic development in the region of cultivation.
柽柳(Solanum betaceum)是一种营养丰富的产品,含有抗氧化成分和预防癌症风险的特性。然而,目前有关使产品多样化并延长柽柳中活性化合物保存时间的加工技术的研究还很少。在这项研究中,我们重点开发了利用酿酒酵母(Angel RV002)进行发酵的苹果酒加工程序。研究了发酵条件,如稀释比例(66 : 34-34 : 66%,w/w)、培养基 pH 值(3.5-5)、总可溶性固形物(TSS 10-26°Brix)和酵母比例(0.6-1.2 克/升)。从发酵的第 0 天到第 5 天,对乙醇浓度、pH 值、TSS、可滴定酸度、总糖含量和还原糖含量进行了评估。在 50 :在罗望子汁与水的稀释比例为 50 : 50(%,w/w)、pH 值为 4.5、TSS 为 22°Brix、发酵过程中添加 0.6 克/升酵母的条件下,发酵 4 天后乙醇浓度达到 7.54±0.11(%,v/v)。此外,产品的 pH 值保持在中等偏低的水平(pH 值为 4.16)。最终产品显示出较高的含糖量和溶解营养成分。这项研究的结果可作为生产罗望子苹果酒的基础,有助于实现产品多样化,提高罗望子的价值,促进种植地区的经济发展。
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引用次数: 0
The Identification of Yak Meat Using Loop-Mediated Isothermal Amplification Method Coupled with Hydroxy Naphthol Blue for the Prevention of Food Fraud 利用环路介导等温扩增法和羟基萘酚蓝鉴定牦牛肉以防食品欺诈
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-30 DOI: 10.1155/2024/6549138
Wenwei Zhao, Yufan Tan, Shanshan Wang, Xiaoxiang Zhu, Jingyu Jiang, Hongbo Zhu, Peihan Zhuang, Wenyi Cheng, Charles S. Brennan, Zhina Yin

Objective. Yak is found in the Qinghai-Tibet Plateau and represents a meat of high nutritional value and good flavor. However, the production of yak is limited, and yak meat adulteration is a growing concern in the marketplace. To protect consumer rights and prevent unfair competition, it is necessary to use an efficient assay to identify the species of yak meat rapidly and accurately being sold. Methods. Loop-mediated isothermal amplification (LAMP) combined with hydroxy naphthol blue (HNB) was used to identify potential adulterants. The specificity and sensitivity tests of yak-derived components were carried out to achieve the monitoring of yak-derived components. Results. The optimal color development was achieved with an external primer-to-internal primer ratio of 400 nmol/L : 1200 nmol/L, 1.5 mmol/L dNTP, and 0.32 U/μL Bst DNA polymerase with 5 mmol/L MgSO4 at 62°C amplification temperature. The detection sensitivity of LAMP-HNB for yak-derived DNA was up to 1 pg/μL. Conclusion. The LAMP-HNB assays provided a valuable tool for the identification of yak gene from adulterated meat. This further enabled the LAMP-HNB assay to be applicable in the identification of other meat products.

目的。牦牛产于青藏高原,是一种营养价值高、味道鲜美的肉类。然而,牦牛的产量有限,市场上牦牛肉掺假问题日益严重。为了保护消费者权益,防止不公平竞争,有必要使用一种有效的检测方法来快速、准确地鉴别所售牦牛肉的品种。方法。采用环路介导等温扩增法(LAMP)结合羟基萘酚蓝(HNB)来鉴别潜在的掺假物质。对牦牛源成分进行特异性和灵敏度测试,以实现对牦牛源成分的监测。结果。外部引物与内部引物的比率为 400 nmol/L 时,显色效果最佳:1200 nmol/L、1.5 mmol/L dNTP、0.32 U/μL Bst DNA 聚合酶和 5 mmol/L MgSO4,扩增温度为 62°C。LAMP-HNB 对牦牛源 DNA 的检测灵敏度高达 1 pg/μL。结论LAMP-HNB 检测法为鉴定掺假肉中的牦牛基因提供了一种有价值的工具。这进一步使 LAMP-HNB 检测法适用于其他肉类产品的鉴定。
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引用次数: 0
Development of Plasma-Activated Water (PAW) System: Molecular Dynamics Simulation and Experimental Study on Almond Aflatoxins 等离子体活化水(PAW)系统的开发:杏仁黄曲霉毒素的分子动力学模拟与实验研究
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-01-19 DOI: 10.1155/2024/3248305
Mohammad Javad Aarabi, Sajad Rostami, Bahram Hosseinzadeh Samani, Firouzeh Nazari

Almonds play a significant role in Iran’s economy, and factors that threaten their market, such as aflatoxins, need careful consideration. Plasma-activated water (PAW) is a new method that has antioxidant activity and can eliminate toxins and microbial agents, making it a suitable solution for removing aflatoxins from almonds. In a study, the continuous PAW production system was used to control and remove almond aflatoxins, and molecular dynamics simulation was employed to evaluate the impact of PAW on aflatoxin B1. The study found that reducing the flow rate of plasma-treated water had the greatest effect on reducing aflatoxin concentration, followed by PAW application time, the dose of inoculated toxin, and the air/gas mixture ratio. The use of PAW reduced aflatoxin concentration in almonds by 12% to 56.8%. The simulation results suggested that PAW can affect the structure of aflatoxin B1, change and destroy its activity, and reduce its toxicity. Among the free radicals tested, NO3 was found to be the most effective in degrading aflatoxin B1. This study demonstrates the potential of PAW as a method to remove aflatoxins and enhance the safety of almonds.

杏仁在伊朗经济中发挥着重要作用,因此需要认真考虑黄曲霉毒素等威胁其市场的因素。等离子活化水(PAW)是一种新方法,它具有抗氧化活性,可以消除毒素和微生物菌剂,是去除杏仁中黄曲霉毒素的合适解决方案。在一项研究中,利用连续 PAW 生产系统来控制和去除杏仁中的黄曲霉毒素,并采用分子动力学模拟来评估 PAW 对黄曲霉毒素 B1 的影响。研究发现,降低等离子处理水的流速对降低黄曲霉毒素浓度的影响最大,其次是使用 PAW 的时间、接种毒素的剂量和空气/气体混合比。使用 PAW 可使杏仁中的黄曲霉毒素浓度降低 12% 至 56.8%。模拟结果表明,PAW 能影响黄曲霉毒素 B1 的结构,改变和破坏其活性,降低其毒性。在测试的自由基中,NO3- 对降解黄曲霉毒素 B1 最有效。这项研究证明了 PAW 作为一种去除黄曲霉毒素和提高杏仁安全性的方法的潜力。
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引用次数: 0
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Journal of Food Processing and Preservation
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