Pub Date : 2024-08-10DOI: 10.1007/s10895-024-03874-8
Rohitash Kumar, Vimal K Bhardwaj
This study employed a green microwave synthesis technique to produce carbon quantum dots (CQDs) from araucaria heterophylla gum extract. The produced CQDs emit a distinct blue fluorescent light, contributing a remarkable quantum yield of 14.69%. Their average particle size measures at 1.62 ± 0.39 nm. Furthermore, these CQDs demonstrate excellent water solubility and maintain high fluorescence stability despite ionic strength, pH and time variations. Moreover, we present here for the first time that the synthesized CQDs demonstrate a rapid, exceptionally sensitive, and discerning fluorescence quenching phenomenon (IFE) concerning Cefprozil (CPR). The fluorescent probe was sensitive and specific with good linear relationships for CPR in the 0-18 µM range. The limit of detection for relationships for CPR was 2.51 µM. This study provides novel opportunities for producing high-quality luminescent CQDs that meet the requirements for various biological and environmental applications.
{"title":"Microwave Synthesis of Fluorescent Carbon Quantum dots from Araucaria Heterophylla Gum: Application in Drug Detection.","authors":"Rohitash Kumar, Vimal K Bhardwaj","doi":"10.1007/s10895-024-03874-8","DOIUrl":"https://doi.org/10.1007/s10895-024-03874-8","url":null,"abstract":"<p><p>This study employed a green microwave synthesis technique to produce carbon quantum dots (CQDs) from araucaria heterophylla gum extract. The produced CQDs emit a distinct blue fluorescent light, contributing a remarkable quantum yield of 14.69%. Their average particle size measures at 1.62 ± 0.39 nm. Furthermore, these CQDs demonstrate excellent water solubility and maintain high fluorescence stability despite ionic strength, pH and time variations. Moreover, we present here for the first time that the synthesized CQDs demonstrate a rapid, exceptionally sensitive, and discerning fluorescence quenching phenomenon (IFE) concerning Cefprozil (CPR). The fluorescent probe was sensitive and specific with good linear relationships for CPR in the 0-18 µM range. The limit of detection for relationships for CPR was 2.51 µM. This study provides novel opportunities for producing high-quality luminescent CQDs that meet the requirements for various biological and environmental applications.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-10DOI: 10.1007/s10895-024-03889-1
Ansh Jaswal, Suman Swami, Ajay Saini
Mercury is known as a highly toxic metal that is poisonous even if present in a trace amount. Generally, it enters in the food chain (especially fish) and water resources via different pathways and leads to harmful effects. Owing to the detrimental nature of the metal, traditionally several methods were employed by researchers for regular monitoring of the mercury metal ions. However, these methods are associated with many limitations like high cost of technical expertise, and intricacy of the detection procedure. So, using these methods to detect mercury ions in real time is challenging. Therefore, in recent years fluorescent-based analytical tools emerged rapidly. Among the various fluorescent organic scaffolds, coumarin has been scorching, owing to quick response, light stability, high sensitivity, good selectivity, excellent fluorescence intensity, and fluorescence quantum yield. This review provides a deep dive into the coumarin-derived chemo-sensors development throughout 2015-2023. We anticipate that the review will assist to broad scientific community as a reference document to design more interesting sensors.
{"title":"Mercury (Hg<sup>2+</sup>) Sensing Using Coumarin-Derived Fluorescent Chemo-Sensors: An Intuitive Development from 2015 to 2023.","authors":"Ansh Jaswal, Suman Swami, Ajay Saini","doi":"10.1007/s10895-024-03889-1","DOIUrl":"https://doi.org/10.1007/s10895-024-03889-1","url":null,"abstract":"<p><p>Mercury is known as a highly toxic metal that is poisonous even if present in a trace amount. Generally, it enters in the food chain (especially fish) and water resources via different pathways and leads to harmful effects. Owing to the detrimental nature of the metal, traditionally several methods were employed by researchers for regular monitoring of the mercury metal ions. However, these methods are associated with many limitations like high cost of technical expertise, and intricacy of the detection procedure. So, using these methods to detect mercury ions in real time is challenging. Therefore, in recent years fluorescent-based analytical tools emerged rapidly. Among the various fluorescent organic scaffolds, coumarin has been scorching, owing to quick response, light stability, high sensitivity, good selectivity, excellent fluorescence intensity, and fluorescence quantum yield. This review provides a deep dive into the coumarin-derived chemo-sensors development throughout 2015-2023. We anticipate that the review will assist to broad scientific community as a reference document to design more interesting sensors.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-10DOI: 10.1007/s10895-024-03897-1
I Hamzi, Y Mered, B Mostefa-Kara
The selective detection of Zn2⁺ and Fe2⁺ ions is critical in environmental and biological studies. Schiff base chemosensors hold promise, but exploration of thiophene-derived variants remains limited. This work introduces a novel thiophene-derived Schiff base sensor (TBH), synthesized through the condensation reaction of thiophene-2-carboxaldehyde with benzil-bis-hydrazone, for the selective detection of Zn2⁺ and Fe2⁺ ions. TBH exhibits remarkable selectivity, with a significant 185-fold fluorescence enhancement for Zn2⁺ and complete quenching 99% for Fe2⁺, allowing for distinct detection of both ions. Notably, TBH demonstrates high binding affinity towards Zn2⁺ and Fe2⁺, even in the presence of competing cations, forming stable 1:1 complexes. This finding is supported by absorption and emission titration studies and FT-IR analysis as well. This easily synthesized, rapid and cost-effective sensor offers a promising approach for sensitive and differentiated dual detection of Zn2⁺ and Fe2⁺ in environmental and biological systems.
{"title":"Highly Sensitive and Selective Recognition of Zn<sup>2</sup>⁺ and Fe<sup>2</sup>⁺ Ions Using a Novel Thiophene-Derived Hydrazone Dual Fluorometric Sensor.","authors":"I Hamzi, Y Mered, B Mostefa-Kara","doi":"10.1007/s10895-024-03897-1","DOIUrl":"https://doi.org/10.1007/s10895-024-03897-1","url":null,"abstract":"<p><p>The selective detection of Zn<sup>2</sup>⁺ and Fe<sup>2</sup>⁺ ions is critical in environmental and biological studies. Schiff base chemosensors hold promise, but exploration of thiophene-derived variants remains limited. This work introduces a novel thiophene-derived Schiff base sensor (TBH), synthesized through the condensation reaction of thiophene-2-carboxaldehyde with benzil-bis-hydrazone, for the selective detection of Zn<sup>2</sup>⁺ and Fe<sup>2</sup>⁺ ions. TBH exhibits remarkable selectivity, with a significant 185-fold fluorescence enhancement for Zn<sup>2</sup>⁺ and complete quenching 99% for Fe<sup>2</sup>⁺, allowing for distinct detection of both ions. Notably, TBH demonstrates high binding affinity towards Zn<sup>2</sup>⁺ and Fe<sup>2</sup>⁺, even in the presence of competing cations, forming stable 1:1 complexes. This finding is supported by absorption and emission titration studies and FT-IR analysis as well. This easily synthesized, rapid and cost-effective sensor offers a promising approach for sensitive and differentiated dual detection of Zn<sup>2</sup>⁺ and Fe<sup>2</sup>⁺ in environmental and biological systems.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We have synthesized a one-pot, three-component pyran-based fluorescence chemosensor using onion extract as a green catalyst. The confirmed structure of the 1:2 binding of receptor SPR-2-picric acid adduct revealed that the pyran-based receptor accommodated two guest picric acid molecules through non-covalent interactions. UV-Vis and fluorescence spectroscopy show high selectivity and sensitivity towards picric acid. The 1D/2D NMR and Job's plot analysis show the complexation and stoichiometric binding of the receptor SPR-2 with picric acid are 1:2. The 1H NMR spectral studies confirm that the formation of receptor SPR-2-picric acid adduct via weak hydrogen bonding. The cooperativity of the receptor SPR-2-picric acid adduct shows negative cooperativity due to the weak hydrogen bonding of receptor SPR-2 and picric acid. Further, the density functional theory (DFT) confirmed the molecular level interaction of the SPR-2 and receptor SPR-2-Picric acid adduct. The receptor was effectively used to assess picric acid concentrations in real water samples.
{"title":"5-Oxo-7,7-Dimethyl-5,6,7,8-Tetrahydro-4-H-Chromene Bearing N, N-Dimethylaniline as Turn-Off Fluorescent Chemosensor for Picric Acid in Real Water Sample.","authors":"Eswaran Rajendran, Selvaraj Loganathan, Ramasamy Santhiya, Gandhi Sivaraman, Muthu Seenivasa Perumal","doi":"10.1007/s10895-024-03886-4","DOIUrl":"https://doi.org/10.1007/s10895-024-03886-4","url":null,"abstract":"<p><p>We have synthesized a one-pot, three-component pyran-based fluorescence chemosensor using onion extract as a green catalyst. The confirmed structure of the 1:2 binding of receptor SPR-2-picric acid adduct revealed that the pyran-based receptor accommodated two guest picric acid molecules through non-covalent interactions. UV-Vis and fluorescence spectroscopy show high selectivity and sensitivity towards picric acid. The 1D/2D NMR and Job's plot analysis show the complexation and stoichiometric binding of the receptor SPR-2 with picric acid are 1:2. The <sup>1</sup>H NMR spectral studies confirm that the formation of receptor SPR-2-picric acid adduct via weak hydrogen bonding. The cooperativity of the receptor SPR-2-picric acid adduct shows negative cooperativity due to the weak hydrogen bonding of receptor SPR-2 and picric acid. Further, the density functional theory (DFT) confirmed the molecular level interaction of the SPR-2 and receptor SPR-2-Picric acid adduct. The receptor was effectively used to assess picric acid concentrations in real water samples.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-09DOI: 10.1007/s10895-024-03878-4
Min Zhou, Fenglei Wang, Yongdong Jin, Shanyong Chen, Chuanqin Xia
Conjugated polymers (CPs)-based near-infrared phototheranostics are receiving increasing attention due to their high molar extinction coefficient, wide emission wavelength, easy preparation and excellent biocompatibility. Herein, several new conjugated polymers with D2-D1-A structures were easily prepared through one-pot coupling using triphenylamine (D2) as well as thiophenes (D1) as electron donors and benzothiadiazole (A) as electron acceptors. Interesting, their optical performance and power conversion efficiency could be tuned by side chains on thiophenes (D1). The introduction of ethylenedioxy into D1 as side chain significantly improves fluorescence imaging brightness, photothermal conversion efficiency and hydrophilicity, and extends emission wavelength, which are beneficial for phototheranostic. The side chain modification provides new opportunity to design efficient phototheranostics without construction new fluorescent skeletons.
{"title":"Improving Photophysical Properties and Hydrophily of Conjugated Polymers Simultaneously by Side-Chain Modification for Near-Infrared Cell Imaging.","authors":"Min Zhou, Fenglei Wang, Yongdong Jin, Shanyong Chen, Chuanqin Xia","doi":"10.1007/s10895-024-03878-4","DOIUrl":"https://doi.org/10.1007/s10895-024-03878-4","url":null,"abstract":"<p><p>Conjugated polymers (CPs)-based near-infrared phototheranostics are receiving increasing attention due to their high molar extinction coefficient, wide emission wavelength, easy preparation and excellent biocompatibility. Herein, several new conjugated polymers with D<sub>2</sub>-D<sub>1</sub>-A structures were easily prepared through one-pot coupling using triphenylamine (D<sub>2</sub>) as well as thiophenes (D<sub>1</sub>) as electron donors and benzothiadiazole (A) as electron acceptors. Interesting, their optical performance and power conversion efficiency could be tuned by side chains on thiophenes (D<sub>1</sub>). The introduction of ethylenedioxy into D<sub>1</sub> as side chain significantly improves fluorescence imaging brightness, photothermal conversion efficiency and hydrophilicity, and extends emission wavelength, which are beneficial for phototheranostic. The side chain modification provides new opportunity to design efficient phototheranostics without construction new fluorescent skeletons.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-07DOI: 10.1007/s10895-024-03854-y
Muhammad Ameerullah Sahudin, Yu Xuan Law, Khairun Nasriah Azmi, Sze Wei Leong, Muhammad Kumayl Abdulwahab, Muhammad Hakim Muhamad Adzrill, Saodah Nurul Nabihah Mohd Zakariah, Nurul Huda Abd Karim
Biogenic amines, produced by bacterial enzymatic reactions in food storage or processing, serve as indicators in food processing industries to assess food quality and freshness. Biogenic amines also often associated with various health problems, including abnormal immune responses and gastrointestinal disease. Previously, salphen base complexes have been reported but still exhibited low fluorescence enhancement upon biogenic amines. This research focused on synthesizing and characterizing new Zn(II) Schiff base complex with indole sidechain to enhance the fluorescence property and exploring their binding behaviour with the biogenic amines, which were phenylethylamine and cadaverine. The Zn(II) indole Schiff base complex's structure was verified by diverse spectroscopic techniques. Then, the binding behaviours between the Zn(II) indole Schiff base complex with the biogenic amines were analyzed using UV-Vis, fluorescence spectroscopy, and Job's plot analysis. UV-Vis binding study results indicated that the synthesized complexes could bind stronger with phenylethylamine than cadaverine, with binding constant, Kb= (8.21 ± 0.58) × 104 M- 1 and (2.506 ± 0.004) × 104 M- 1 respectively. Moreover, Zn(II) indole Schiff base complex-phenylethylamine binding also generated higher fluorescence enhancement than cadaverine, which were 54% and 51% respectively. Based on Job's plot analysis, the complex and biogenic amines were bound in the ratio of 1:1. To conclude, the synthesized complex has promising potential as a sensing material for biogenic amines detection in food. The complex is recommended to be deployed in the development of solid-state fluorescence sensor for biogenic amines detection for monitoring the food spoilage in the food industry in the future.
{"title":"Indole Schiff Base Complex: Synthesis and Optical Binding Investigation with Biogenic Amines.","authors":"Muhammad Ameerullah Sahudin, Yu Xuan Law, Khairun Nasriah Azmi, Sze Wei Leong, Muhammad Kumayl Abdulwahab, Muhammad Hakim Muhamad Adzrill, Saodah Nurul Nabihah Mohd Zakariah, Nurul Huda Abd Karim","doi":"10.1007/s10895-024-03854-y","DOIUrl":"https://doi.org/10.1007/s10895-024-03854-y","url":null,"abstract":"<p><p>Biogenic amines, produced by bacterial enzymatic reactions in food storage or processing, serve as indicators in food processing industries to assess food quality and freshness. Biogenic amines also often associated with various health problems, including abnormal immune responses and gastrointestinal disease. Previously, salphen base complexes have been reported but still exhibited low fluorescence enhancement upon biogenic amines. This research focused on synthesizing and characterizing new Zn(II) Schiff base complex with indole sidechain to enhance the fluorescence property and exploring their binding behaviour with the biogenic amines, which were phenylethylamine and cadaverine. The Zn(II) indole Schiff base complex's structure was verified by diverse spectroscopic techniques. Then, the binding behaviours between the Zn(II) indole Schiff base complex with the biogenic amines were analyzed using UV-Vis, fluorescence spectroscopy, and Job's plot analysis. UV-Vis binding study results indicated that the synthesized complexes could bind stronger with phenylethylamine than cadaverine, with binding constant, K<sub>b</sub>= (8.21 ± 0.58) × 10<sup>4</sup> M<sup>- 1</sup> and (2.506 ± 0.004) × 10<sup>4</sup> M<sup>- 1</sup> respectively. Moreover, Zn(II) indole Schiff base complex-phenylethylamine binding also generated higher fluorescence enhancement than cadaverine, which were 54% and 51% respectively. Based on Job's plot analysis, the complex and biogenic amines were bound in the ratio of 1:1. To conclude, the synthesized complex has promising potential as a sensing material for biogenic amines detection in food. The complex is recommended to be deployed in the development of solid-state fluorescence sensor for biogenic amines detection for monitoring the food spoilage in the food industry in the future.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-05DOI: 10.1007/s10895-024-03839-x
Ahmed Abdulhafez Hamad, Safaa F Saleh, Wael A Mahdi, Sultan Alshehri, Mohamed A El Hamd
The proposed investigation follows a certain methodology to guarantee that the procedure employed is sustainable and green. It is noteworthy to mention that various tools have been implemented as potential indicators of environmental sustainability (greenness and whiteness). From a novelty viewpoint, a new tool, BAGI, for the method's blueness evaluation was applied to the planned method and showed a high applicability score. Fortunately, the WAC concept, which combines ecological and functional variables using the Green/Red/Blue design (RBG 12 tool), identifies the established analytical approach as white. In the planned study, a new, green, simple, nano-trace-sensitive, original fluorimetric methodology was established to analyze and assess midodrine hydrochloride content in different matrices. Midodrine's primary amine moiety reacts with Diacetylmethane/Oxymethylene reagent in an acetate buffer, which leads to generating a fluorescent dihydrolutidine derivative (Hantzsch-named reaction). Consequently, the signal strength of this compound was quantified at 487 nm, with an excitation wavelength of 426 nm. This analysis indicated that the technique exhibited linearity within the range of 0.05 to 1.1 µg mL-1 concentrations, accompanied by remarkably good sensitivity values (LOD and LOQ). The methodology employed in this examination was subjected to validation following the rules recognized by ICH. From the perspective of pharmacy and chemistry, the method presented in this study was successfully employed to analyze commercially available tablets, oral drops, and human fluids. The outcomes obtained demonstrated satisfactory recovery rates without any interference from excipients. Following the USP recommendations, the intended technique was finally implemented to explore the content homogeneity evaluation.
{"title":"Facile Integration of Hanztsch's Switch-Off/On Modeled Fluorogenic Probe for Feasible Tagging and Tracking of the Midodrine Drug in Different Matrices; First Evaluation of the Method's Greenness, Whiteness, Blueness, Quantum Yield, and Tablets' Content Homogeneity.","authors":"Ahmed Abdulhafez Hamad, Safaa F Saleh, Wael A Mahdi, Sultan Alshehri, Mohamed A El Hamd","doi":"10.1007/s10895-024-03839-x","DOIUrl":"https://doi.org/10.1007/s10895-024-03839-x","url":null,"abstract":"<p><p>The proposed investigation follows a certain methodology to guarantee that the procedure employed is sustainable and green. It is noteworthy to mention that various tools have been implemented as potential indicators of environmental sustainability (greenness and whiteness). From a novelty viewpoint, a new tool, BAGI, for the method's blueness evaluation was applied to the planned method and showed a high applicability score. Fortunately, the WAC concept, which combines ecological and functional variables using the Green/Red/Blue design (RBG 12 tool), identifies the established analytical approach as white. In the planned study, a new, green, simple, nano-trace-sensitive, original fluorimetric methodology was established to analyze and assess midodrine hydrochloride content in different matrices. Midodrine's primary amine moiety reacts with Diacetylmethane/Oxymethylene reagent in an acetate buffer, which leads to generating a fluorescent dihydrolutidine derivative (Hantzsch-named reaction). Consequently, the signal strength of this compound was quantified at 487 nm, with an excitation wavelength of 426 nm. This analysis indicated that the technique exhibited linearity within the range of 0.05 to 1.1 µg mL<sup>-1</sup> concentrations, accompanied by remarkably good sensitivity values (LOD and LOQ). The methodology employed in this examination was subjected to validation following the rules recognized by ICH. From the perspective of pharmacy and chemistry, the method presented in this study was successfully employed to analyze commercially available tablets, oral drops, and human fluids. The outcomes obtained demonstrated satisfactory recovery rates without any interference from excipients. Following the USP recommendations, the intended technique was finally implemented to explore the content homogeneity evaluation.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31DOI: 10.1007/s10895-024-03868-6
Shuji Li, Ke Su, Xing Liu, Xiuming Jiang, Guoqiang Xiang, Lijun He
Nitrogen-doped carbon dots (N-CDs) were prepared by self-exothermic procedure using grasshopper powder as a single precursor. The prepared N-CDs not only have excellent fluorescence properties, but also can catalyze and enhance the ultra-weak chemiluminescence of NaHCO3-H2O2. The reaction conditions of NaHCO3-H2O2-N-CDs CL were optimized. Under the optimal experimental conditions, when AA was added to the NaHCO3-H2O2-N-CDs CL system, AA had a significant inhibitory effect on the CL intensity of NaHCO3-H2O2-N-CDs. There was a good linear relationship between the calculated lg(I0/I) and the concentration of AA (C), and the calibration curve equation was lg(I0/I) = 0.03667 C-0.00708 (µM). The established CL analysis method has a detection limit of 0.12 µM for AA and a linear range of 0-50 µM. The selectivity of CL method was evaluated, and the method was successfully applied to the determination of AA in vegetable and fruit samples. The spiked recoveries were between 88.9% and 118.9%, which indicated that the method was simple, rapid, and sensitive, and had great potential in the determination of AA in foods.
{"title":"Nitrogen-Doped Carbon Dots Enhanced Hydrogen Carbonate-Hydrogen Peroxide Chemiluminescent Reaction and its Application in Ascorbic Acid Sensing.","authors":"Shuji Li, Ke Su, Xing Liu, Xiuming Jiang, Guoqiang Xiang, Lijun He","doi":"10.1007/s10895-024-03868-6","DOIUrl":"https://doi.org/10.1007/s10895-024-03868-6","url":null,"abstract":"<p><p>Nitrogen-doped carbon dots (N-CDs) were prepared by self-exothermic procedure using grasshopper powder as a single precursor. The prepared N-CDs not only have excellent fluorescence properties, but also can catalyze and enhance the ultra-weak chemiluminescence of NaHCO<sub>3</sub>-H<sub>2</sub>O<sub>2</sub>. The reaction conditions of NaHCO<sub>3</sub>-H<sub>2</sub>O<sub>2</sub>-N-CDs CL were optimized. Under the optimal experimental conditions, when AA was added to the NaHCO<sub>3</sub>-H<sub>2</sub>O<sub>2</sub>-N-CDs CL system, AA had a significant inhibitory effect on the CL intensity of NaHCO<sub>3</sub>-H<sub>2</sub>O<sub>2</sub>-N-CDs. There was a good linear relationship between the calculated lg(I0/I) and the concentration of AA (C), and the calibration curve equation was lg(I<sub>0</sub>/I) = 0.03667 C-0.00708 (µM). The established CL analysis method has a detection limit of 0.12 µM for AA and a linear range of 0-50 µM. The selectivity of CL method was evaluated, and the method was successfully applied to the determination of AA in vegetable and fruit samples. The spiked recoveries were between 88.9% and 118.9%, which indicated that the method was simple, rapid, and sensitive, and had great potential in the determination of AA in foods.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.1007/s10895-024-03855-x
Şeyma Nur Ural Baydeniz, Halil İsmet Uçan, Fatih Sevgi, İhsan Obalı, Aslıhan Yılmaz Obalı
π-Conjugated azomethine ligands differing in the naphthalene or phenylmethane-centered core structure and their divalent cobalt, nickel, copper, and zinc metal complexes were prepared and well-characterized by spectral analyses in solid state. Magnetic natures of the complexes were determined by magnetic susceptibility measurements in solid-state. Their remarkable photophysical characteristics were recorded by Uv-vis and Fluorescence spectroscopic techniques. At their excitation wavelenght of 265 nm, all molecules exhibited triple fluorescence emission bands with promising intensities above 673 nm in near infra-red region. Antibacterial and antibiofilm activities of the π-conjugated azomethines are promising for potential applications in medical and healthcare settings. Hence, the antibacterial/antibiofilm activity of the π-conjugated azomethine ligands and their metal complexes against some clinically important bacteria namely Staphylococcus aureus (MSSA), Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis was investigated, and the obtained results have shown that the ligands and complexes had a remarkable antibacterial effect, especially on Proteus mirabilis. Metal complexes have been found to have a significant inhibitory effect on biofilm formation by MRSA, MSSA, and P. mirabilis compared to ligands. The copper (II) complex of ligand-2 showed the highest inhibition percentage, significantly reducing biofilm formation for MRSA and MSSA. Furthermore, cobalt (II) complexes of the ligands selectively inhibited the growth of the opportunistic pathogen P. mirabilis biofilms, indicating that metal complexes might be a good choice for future antibiofilm studies.
{"title":"Highly Fluorescent π-Conjugated Azomethines and Divalent Metal Complexes as Antibacterial and Antibiofilm Nominees.","authors":"Şeyma Nur Ural Baydeniz, Halil İsmet Uçan, Fatih Sevgi, İhsan Obalı, Aslıhan Yılmaz Obalı","doi":"10.1007/s10895-024-03855-x","DOIUrl":"https://doi.org/10.1007/s10895-024-03855-x","url":null,"abstract":"<p><p>π-Conjugated azomethine ligands differing in the naphthalene or phenylmethane-centered core structure and their divalent cobalt, nickel, copper, and zinc metal complexes were prepared and well-characterized by spectral analyses in solid state. Magnetic natures of the complexes were determined by magnetic susceptibility measurements in solid-state. Their remarkable photophysical characteristics were recorded by Uv-vis and Fluorescence spectroscopic techniques. At their excitation wavelenght of 265 nm, all molecules exhibited triple fluorescence emission bands with promising intensities above 673 nm in near infra-red region. Antibacterial and antibiofilm activities of the π-conjugated azomethines are promising for potential applications in medical and healthcare settings. Hence, the antibacterial/antibiofilm activity of the π-conjugated azomethine ligands and their metal complexes against some clinically important bacteria namely Staphylococcus aureus (MSSA), Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis was investigated, and the obtained results have shown that the ligands and complexes had a remarkable antibacterial effect, especially on Proteus mirabilis. Metal complexes have been found to have a significant inhibitory effect on biofilm formation by MRSA, MSSA, and P. mirabilis compared to ligands. The copper (II) complex of ligand-2 showed the highest inhibition percentage, significantly reducing biofilm formation for MRSA and MSSA. Furthermore, cobalt (II) complexes of the ligands selectively inhibited the growth of the opportunistic pathogen P. mirabilis biofilms, indicating that metal complexes might be a good choice for future antibiofilm studies.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-29DOI: 10.1007/s10895-024-03866-8
Debi D Pant
This paper reports the influence of surface charge of the micelles on to the photophysical properties of a cinchonine dication (C2+) fluorophore in anionic, sodium dodecylsulphate (SDS), surfactant at premicellar, micellar and post-micellar concentrations in aqueous phase at room temperature. The magnitude of edge excitation red shift (EERS) in the fluorescence maximum of C2+ in bulk water solution is 1897 cm- 1 whereas, in the case of SDS it is observed to be 1984 cm- 1. The fluorescence decay curve of C2+ fits with multi exponential functions in the micellar system. The increase in lifetime of C2+ in SDS has been attributed to the increase in radiative rate due to the incorporation of C2+ at the micelle -water interface. The value of dynamic quenching constant determined is 16.9 M- 1. The location of the probe molecule in micellar systems has been justified by a variety of spectral parameters such as dielectric constant, ET (30), viscosity, EERS, average fluorescence decay time, radiative and non-radiative rate constants. All experimental results suggest that the C2+ molecule binds strongly with the SDS micelles and resides at micellar-water interface. The binding constant (Kb) calculated (3.85 × 105 M- 1) for C2+ in SDS revealed that the electrostatic forces mediate charge probe-micelle association.
{"title":"Steady State and Time-Resolved Fluorescence Spectroscopy of Cinchonine Dication in Sodium Dodecylsulphate Micellar System.","authors":"Debi D Pant","doi":"10.1007/s10895-024-03866-8","DOIUrl":"https://doi.org/10.1007/s10895-024-03866-8","url":null,"abstract":"<p><p>This paper reports the influence of surface charge of the micelles on to the photophysical properties of a cinchonine dication (C<sup>2+</sup>) fluorophore in anionic, sodium dodecylsulphate (SDS), surfactant at premicellar, micellar and post-micellar concentrations in aqueous phase at room temperature. The magnitude of edge excitation red shift (EERS) in the fluorescence maximum of C<sup>2+</sup> in bulk water solution is 1897 cm<sup>- 1</sup> whereas, in the case of SDS it is observed to be 1984 cm<sup>- 1</sup>. The fluorescence decay curve of C<sup>2+</sup> fits with multi exponential functions in the micellar system. The increase in lifetime of C<sup>2+</sup> in SDS has been attributed to the increase in radiative rate due to the incorporation of C<sup>2+</sup> at the micelle -water interface. The value of dynamic quenching constant determined is 16.9 M<sup>- 1</sup>. The location of the probe molecule in micellar systems has been justified by a variety of spectral parameters such as dielectric constant, E<sub>T</sub> (30), viscosity, EERS, average fluorescence decay time, radiative and non-radiative rate constants. All experimental results suggest that the C<sup>2+</sup> molecule binds strongly with the SDS micelles and resides at micellar-water interface. The binding constant (K<sub>b</sub>) calculated (3.85 × 10<sup>5</sup> M<sup>- 1</sup>) for C<sup>2+</sup> in SDS revealed that the electrostatic forces mediate charge probe-micelle association.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}