Oxidation stability is a key property of olive oil quality and is affected by different antioxidant compounds whose levels may be influenced by several factors such as cultivar and place of production. Polyphenols, carotenoids, chlorophylls, fatty acids levels and some volatiles were correlated to oxidative stability in olive oils in five samples studied. Total polyphenols and saturated to polyunsaturated fatty acids (and/or oleic to linoleic acid ratio) ratio were shown to be the major parameters in oil antioxidant stability, according to analysis of variance and principal component analysis. The hexanal/E-2-hexenal ratio is a very important indicator of the freshness of the oils and can estimate their oxidation degree.
PRACTICAL APPLICATIONS
The present study gives information about the importance and the variability of some Tunisian olive oil varieties in both their quality (high oxidative stability) and good organoleptic properties which could be appreciated by consumers.
摘要:氧化稳定性是橄榄油品质的一个关键特性，它受到不同抗氧化化合物的影响，这些抗氧化化合物的水平可能受到多种因素的影响，如品种和产地。在研究的五个样品中，多酚、类胡萝卜素、叶绿素、脂肪酸水平和一些挥发物与橄榄油的氧化稳定性有关。方差分析和主成分分析表明，总多酚和饱和与多不饱和脂肪酸(和/或油酸与亚油酸的比值)是影响油脂抗氧化稳定性的主要参数。己醛/ e -2-己烯醛比值是油脂新鲜度的重要指标，可用于评价油脂的氧化程度。实际应用本研究提供了一些突尼斯橄榄油品种在质量(高氧化稳定性)和良好的感官特性方面的重要性和可变性的信息，这些可以被消费者所欣赏。
{"title":"DISCRIMINATION OF SOME TUNISIAN OLIVE OIL VARIETIES ACCORDING TO THEIR OXIDATIVE STABILITY, VOLATILES COMPOUNDS AND CHEMOMETRIC ANALYSIS","authors":"MANEL ISSAOUI, KAOUTHER BEN HASSINE, GUIDO FLAMINI, FATEN BRAHMI, HECHMI CHEHAB, YOUSSEF AOUNI, BELIGH MECHRI, MOKHTAR ZARROUK, MOHAMED HAMMAMI","doi":"10.1111/j.1745-4522.2009.01139.x","DOIUrl":"10.1111/j.1745-4522.2009.01139.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Oxidation stability is a key property of olive oil quality and is affected by different antioxidant compounds whose levels may be influenced by several factors such as cultivar and place of production. Polyphenols, carotenoids, chlorophylls, fatty acids levels and some volatiles were correlated to oxidative stability in olive oils in five samples studied. Total polyphenols and saturated to polyunsaturated fatty acids (and/or oleic to linoleic acid ratio) ratio were shown to be the major parameters in oil antioxidant stability, according to analysis of variance and principal component analysis. The hexanal/E-2-hexenal ratio is a very important indicator of the freshness of the oils and can estimate their oxidation degree.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>The present study gives information about the importance and the variability of some Tunisian olive oil varieties in both their quality (high oxidative stability) and good organoleptic properties which could be appreciated by consumers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01139.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63565350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-19DOI: 10.1111/j.1745-4522.2009.01144.x
GHANYA AL-NAQEEB, MAZNAH ISMAIL
ABSTRACT
Thymoquinone (TQ) rich fraction (TQRF) extracted from Nigella sativa seeds using a supercritical fluid extraction technique was prepared. The regulatory effects of TQRF at 80 µg/mL and commercial TQ at 2 µg/mL on apolipoprotein B100 (Apo B100) and apolipoprotein A-1 (Apo A-1) genes in the presence or absence of 25-hydroxycholesterol (25OH), were investigated in human HepG2 cell line using quantitative real-time polymerase chain reaction. Incubating HepG2 cells in 10% human lipoprotein deficient serum (HLPDS) for 24 h in the presence of 2 µg/mL 25OH showed a significant increase in Apo B100 mRNA expression level by twofold compared to the control cells; on the other hand, no significant change in Apo A-1 mRNA level was observed. When cells were incubated with HLPDS in the absence of 25OH and treated with TQRF and TQ, the mRNA level of Apo B100 was down-regulated by 70 and 49%, respectively, in TQRF and TQ treated cells compared to untreated cells. Apo A-1 gene was up-regulated by four- and twofold in TQRF and TQ treated cells, respectively, compared to that observed in untreated cells. The present study clearly shows that TQRF and TQ are effective in regulating Apo A-1 and Apo B100 genes that influence cholesterol metabolism in HepG2 cells.
PRACTICAL APPLICATIONS
Cardiovascular diseases (CVDs), including coronary heart disease, are considered as frequent cause of most deaths in the world. Hypercholesterolemia is the major risk of CVD. The plasma levels of Apo B100 and Apo A-1 have been reported to be a risk of developing CVD. Natural products that are able to up-regulate Apo A-1 and down-regulate Apo B100 genes are candidates for preventing and treating hypercholesterolemia. The regulatory effect of thymoquinone rich fraction extracted from Nigella sativa seeds using supercritical fluid extraction on Apo A-1 and Apo B100 genes may play an important role in controlling the plasma cholesterol level, and thus, may reduce the risk of CVD.
{"title":"REGULATION OF APOLIPOPROTEIN A-1 AND APOLIPOPROTEIN B100 GENES BY THYMOQUINONE RICH FRACTION AND THYMOQUINONE IN HEPG2 CELLS","authors":"GHANYA AL-NAQEEB, MAZNAH ISMAIL","doi":"10.1111/j.1745-4522.2009.01144.x","DOIUrl":"10.1111/j.1745-4522.2009.01144.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Thymoquinone (TQ) rich fraction (TQRF) extracted from Nigella sativa seeds using a supercritical fluid extraction technique was prepared. The regulatory effects of TQRF at 80 µg/mL and commercial TQ at 2 µg/mL on apolipoprotein B100 (Apo B100) and apolipoprotein A-1 (Apo A-1) genes in the presence or absence of 25-hydroxycholesterol (25OH), were investigated in human HepG2 cell line using quantitative real-time polymerase chain reaction. Incubating HepG2 cells in 10% human lipoprotein deficient serum (HLPDS) for 24 h in the presence of 2 µg/mL 25OH showed a significant increase in Apo B100 mRNA expression level by twofold compared to the control cells; on the other hand, no significant change in Apo A-1 mRNA level was observed. When cells were incubated with HLPDS in the absence of 25OH and treated with TQRF and TQ, the mRNA level of Apo B100 was down-regulated by 70 and 49%, respectively, in TQRF and TQ treated cells compared to untreated cells. Apo A-1 gene was up-regulated by four- and twofold in TQRF and TQ treated cells, respectively, compared to that observed in untreated cells. The present study clearly shows that TQRF and TQ are effective in regulating Apo A-1 and Apo B100 genes that influence cholesterol metabolism in HepG2 cells.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Cardiovascular diseases (CVDs), including coronary heart disease, are considered as frequent cause of most deaths in the world. Hypercholesterolemia is the major risk of CVD. The plasma levels of Apo B100 and Apo A-1 have been reported to be a risk of developing CVD. Natural products that are able to up-regulate Apo A-1 and down-regulate Apo B100 genes are candidates for preventing and treating hypercholesterolemia. The regulatory effect of thymoquinone rich fraction extracted from <i>Nigella sativa</i> seeds using supercritical fluid extraction on Apo A-1 and Apo B100 genes may play an important role in controlling the plasma cholesterol level, and thus, may reduce the risk of CVD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01144.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63565196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-19DOI: 10.1111/j.1745-4522.2009.01138.x
GAO-FENG YUAN, ANDREW J. SINCLAIR, HAI-YAN SUN, DUO LI
ABSTRACT
The influence of 1% alpha-eleostearic acid (α-ESA, cis9,trans11,trans 13-18:3) and 1% punicic acid (PA, cis9,trans11,cis13-18:3) on fatty acid composition in mouse tissues was compared with conjugated linoleic acid (CLA, mixture of primarily cis9,trans11- and trans10,cis12-18:2) in the present study. The content (% total fatty acids) of 18:2n-6 was significantly reduced in the heart and adipose tissues, and total polyunsaturated fatty acids (PUFAs) and n-6 PUFA were significantly reduced in adipose tissue by α-ESA, PA and CLA feeding. The content of 22:6n-3 and total n-3 PUFA were significantly increased in the liver, kidney and heart by PA feeding, but not by α-ESA. In contrast to PA, supplementation with CLA significantly decreased 22:6n-3 in the liver, kidney and heart. The content of 20:4n-6 was significantly decreased in the liver and kidney by CLA feeding, but not by α-ESA and PA. The present results indicate that α-ESA, PA and CLA have differential effects on 22:6n-3 and 20:4n-6 content in mouse tissues.
PRACTICAL APPLICATIONS
Conjugated linolenic acid (CLnA), a group of octadecatrienoic acid isomers with a conjugated triene system, has been reported to exhibit favorable physiological effects, including anticancer properties and regulation of lipid metabolism. Punicic acid and alpha-eleostearic acid, two isomers of CLnA, have been shown to convert into cis9,trans11-18:2 in vivo. The effect of CLnA on fatty acid composition in mouse tissues was investigated in comparison with CLA mixtures in the present study. The data obtained here could provide information for the potential application of CLnA-containing seeds as functional food ingredients, a natural source of endogenously formed cis9,trans11-18:2 and a dietary feeding strategy to beneficially modify the fatty acid composition of animal tissues.
{"title":"FATTY ACID COMPOSITION IN TISSUES OF MICE FED DIETS CONTAINING CONJUGATED LINOLENIC ACID AND CONJUGATED LINOLEIC ACID","authors":"GAO-FENG YUAN, ANDREW J. SINCLAIR, HAI-YAN SUN, DUO LI","doi":"10.1111/j.1745-4522.2009.01138.x","DOIUrl":"10.1111/j.1745-4522.2009.01138.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The influence of 1% alpha-eleostearic acid (α-ESA, cis9,trans11,trans 13-18:3) and 1% punicic acid (PA, cis9,trans11,cis13-18:3) on fatty acid composition in mouse tissues was compared with conjugated linoleic acid (CLA, mixture of primarily cis9,trans11- and trans10,cis12-18:2) in the present study. The content (% total fatty acids) of 18:2n-6 was significantly reduced in the heart and adipose tissues, and total polyunsaturated fatty acids (PUFAs) and n-6 PUFA were significantly reduced in adipose tissue by α-ESA, PA and CLA feeding. The content of 22:6n-3 and total n-3 PUFA were significantly increased in the liver, kidney and heart by PA feeding, but not by α-ESA. In contrast to PA, supplementation with CLA significantly decreased 22:6n-3 in the liver, kidney and heart. The content of 20:4n-6 was significantly decreased in the liver and kidney by CLA feeding, but not by α-ESA and PA. The present results indicate that α-ESA, PA and CLA have differential effects on 22:6n-3 and 20:4n-6 content in mouse tissues.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Conjugated linolenic acid (CLnA), a group of octadecatrienoic acid isomers with a conjugated triene system, has been reported to exhibit favorable physiological effects, including anticancer properties and regulation of lipid metabolism. Punicic acid and alpha-eleostearic acid, two isomers of CLnA, have been shown to convert into <i>cis</i>9,<i>trans</i>11-18:2 <i>in vivo</i>. The effect of CLnA on fatty acid composition in mouse tissues was investigated in comparison with CLA mixtures in the present study. The data obtained here could provide information for the potential application of CLnA-containing seeds as functional food ingredients, a natural source of endogenously formed <i>cis</i>9,<i>trans</i>11-18:2 and a dietary feeding strategy to beneficially modify the fatty acid composition of animal tissues.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01138.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63565324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-19DOI: 10.1111/j.1745-4522.2009.01140.x
BERTRAND MATTHÄUS, MEHMET MUSA ÖZCAN
ABSTRACT
The oil content as well as the fatty acid and tocopherol composition of kernels from 15 Prunus spp. varieties from Turkey were determined. The oil yields from these kernels varied from 46.3 to 55.5%. The main fatty acids of Prunus spp. kernel oils were oleic acid (43.9–78.5%), linoleic acid (9.7–37%) and palmitic acid (4.9–7.3%). The total amount of vitamin-E-active compounds in the oils varied between 62.9 and 439.9 mg/kg. The predominant tocopherol in most kernel oils was γ-tocopherol. Only two varieties of P. amygdalus and one variety of P. persica showed α-tocopherol as the main vitamin-E-active compound. The composition of the oils was 9–164.5 mg/kg α-tocopherol, 21.5–41.6 mg/kg α-tocotrienol, 1.6–330.2 mg/kg γ-tocopherol and 0–39.1 mg/kg δ-tocopherol. From the results of the present study, it can be concluded that the kernels of the investigated species of Prunus fruits from Turkey may serve potential sources of valuable oil that might be used for edible and other industrial applications.
PRACTICAL APPLICATIONS
The search for new sources of vegetable oils is an ongoing challenge and the further utilization of by-products from the food processing industry is an interesting option in this field. Seed oils from Prunus species contain high amounts of recommended monounsaturated oleic acid moderate contents of linoleic acid and low amounts of saturated fatty acids that may result in more favorable oil than olive oil with regard to their fatty acid compositions. Additionally, the oils contain vitamin-E-active compounds. Both fatty acid composition and vitamin-E-active compounds may justify the further processing of seeds from Prunus species for the production of oil for food and pharmaceutical applications.
{"title":"FATTY ACIDS AND TOCOPHEROL CONTENTS OF SOME PRUNUS SPP. KERNEL OILS","authors":"BERTRAND MATTHÄUS, MEHMET MUSA ÖZCAN","doi":"10.1111/j.1745-4522.2009.01140.x","DOIUrl":"10.1111/j.1745-4522.2009.01140.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The oil content as well as the fatty acid and tocopherol composition of kernels from 15</i> Prunus <i>spp. varieties from Turkey were determined. The oil yields from these kernels varied from 46.3 to 55.5%. The main fatty acids of</i> Prunus <i>spp. kernel oils were oleic acid (43.9–78.5%), linoleic acid (9.7–37%) and palmitic acid (4.9–7.3%). The total amount of vitamin-E-active compounds in the oils varied between 62.9 and 439.9 mg/kg. The predominant tocopherol in most kernel oils was γ-tocopherol. Only two varieties of P. amygdalus and one variety of P. persica showed α-tocopherol as the main vitamin-E-active compound. The composition of the oils was 9–164.5 mg/kg α-tocopherol, 21.5–41.6 mg/kg α-tocotrienol, 1.6–330.2 mg/kg γ-tocopherol and 0–39.1 mg/kg δ-tocopherol. From the results of the present study, it can be concluded that the kernels of the investigated species of</i> Prunus <i>fruits from Turkey may serve potential sources of valuable oil that might be used for edible and other industrial applications.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>The search for new sources of vegetable oils is an ongoing challenge and the further utilization of by-products from the food processing industry is an interesting option in this field. Seed oils from <i>Prunus</i> species contain high amounts of recommended monounsaturated oleic acid moderate contents of linoleic acid and low amounts of saturated fatty acids that may result in more favorable oil than olive oil with regard to their fatty acid compositions. Additionally, the oils contain vitamin-E-active compounds. Both fatty acid composition and vitamin-E-active compounds may justify the further processing of seeds from <i>Prunus</i> species for the production of oil for food and pharmaceutical applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01140.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63564949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of dietary plant cerebroside on colon gene expression by DNA microarray analysis in 1,2-dimethylhydrazine (DMH)-treated mice were investigated. After 9 weeks of feeding with DMH, decreases and increases in the levels of expression of 110 and 145 genes were detected, respectively. Especially, the expression of Soggy-1, which suppresses the Wnt signaling pathway, was increased, while that of Ras-associated protein, which induces the mitogen-activated protein (MAP)-kinase pathway and is responsible for the development of aberrant crypt foci (ACF), was decreased. The results of the present study indicated that dietary cerebroside in DMH-treated mice regulates the Wnt signaling and MAP-kinase pathways, and prevents the development of ACF in the large intestine.
PRACTICAL APPLICATIONS
Dietary plant cerebroside increased expression of Soggy-1 mRNA, which suppresses the Wnt signaling pathway, and decreased expression ofRas-associated protein, which induces the MAP-kinase pathway in 1,2-dimethylhydrazine (DMH)-treated mice. The results of the present study indicated that dietary cerebroside in DMH-treated mice regulates the Wnt signaling and MAP-kinase pathways, and prevents the development of aberrant crypt foci in the large intestine, thus being of potential use for nutraceutical applications.
{"title":"EFFECTS OF DIETARY PLANT CEREBROSIDE ON GENE EXPRESSION IN THE LARGE INTESTINE OF 1,2-DIMETHYLHYDRAZINE (DMH)-TREATED MICE DETERMINED BY DNA MICROARRAY ANALYSIS","authors":"MIKIO KINOSHITA, KAZUHIKO AIDA, YOSHIHIKO TOKUJI, TATSUYA SUGAWARA, MASAO OHNISHI","doi":"10.1111/j.1745-4522.2009.01141.x","DOIUrl":"10.1111/j.1745-4522.2009.01141.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The effects of dietary plant cerebroside on colon gene expression by DNA microarray analysis in 1,2-dimethylhydrazine (DMH)-treated mice were investigated. After 9 weeks of feeding with DMH, decreases and increases in the levels of expression of 110 and 145 genes were detected, respectively. Especially, the expression of Soggy-1, which suppresses the Wnt signaling pathway, was increased, while that of Ras-associated protein, which induces the mitogen-activated protein (MAP)-kinase pathway and is responsible for the development of aberrant crypt foci (ACF), was decreased. The results of the present study indicated that dietary cerebroside in DMH-treated mice regulates the Wnt signaling and MAP-kinase pathways, and prevents the development of ACF in the large intestine.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Dietary plant cerebroside increased expression of Soggy-1 mRNA, which suppresses the Wnt signaling pathway, and decreased expression ofRas-associated protein, which induces the MAP-kinase pathway in 1,2-dimethylhydrazine (DMH)-treated mice. The results of the present study indicated that dietary cerebroside in DMH-treated mice regulates the Wnt signaling and MAP-kinase pathways, and prevents the development of aberrant crypt foci in the large intestine, thus being of potential use for nutraceutical applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01141.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63565034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-05-19DOI: 10.1111/j.1745-4522.2009.01145.x
MARIO LORENZ, VERENA STANGL, CHRISTINE JACOB, KERSTIN DAEMEN, VOLKER BÖHM, KATI FRÖHLICH, GERT BAUMANN, KARL STANGL, ROSSELLA SIMONE, PAOLA PALOZZA
ABSTRACT
Lycopene most likely contributes to the positive health effects of tomatoes on the cardiovascular system. However, elucidation of underlying cellular mechanisms is hampered by the intricate solubility of lycopene in aqueous solutions. Cells relevant to the cardiovascular system, including bovine aortic endothelial cells (BAECs), the monocytic cell line THP-1, and RAT-1 fibroblasts, were treated for various time periods (0–72 h) with different concentrations of lycopene (1, 5, and 10 µM), solubilized either in tetrahydrofuran (THF) or micelles as solvents. Incubation of all three cell types led to a concentration- and time-dependent increase in cellular lycopene content. Both vehicles tested, THF and micelles, proved equally effective in the delivery of lycopene to cells. A marked difference in the amount of lycopene incorporated was observed among the various cell types. Compared with THP-1 cells, the uptake of lycopene using both solvents was higher in BAECs and RAT-1 fibroblasts for all concentrations and time points tested.
PRACTICAL APPLICATIONS
Epidemiological data indicate a beneficial effect for consumption of tomato products in the prevention of cardiovascular diseases. Only limited data are available on the cellular uptake of lycopene in vascular cells. Lycopene was successfully delivered to different cells relevant for the cardiovascular system. These results represent an important prerequisite for the study of molecular and cellular mechanisms by which lycopene may exert its beneficial effects on the cardiovascular system.
{"title":"DELIVERY OF LYCOPENE TO PHYSIOLOGICALLY RELEVANT VASCULAR CELLS","authors":"MARIO LORENZ, VERENA STANGL, CHRISTINE JACOB, KERSTIN DAEMEN, VOLKER BÖHM, KATI FRÖHLICH, GERT BAUMANN, KARL STANGL, ROSSELLA SIMONE, PAOLA PALOZZA","doi":"10.1111/j.1745-4522.2009.01145.x","DOIUrl":"10.1111/j.1745-4522.2009.01145.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Lycopene most likely contributes to the positive health effects of tomatoes on the cardiovascular system. However, elucidation of underlying cellular mechanisms is hampered by the intricate solubility of lycopene in aqueous solutions. Cells relevant to the cardiovascular system, including bovine aortic endothelial cells (BAECs), the monocytic cell line THP-1, and RAT-1 fibroblasts, were treated for various time periods (0–72 h) with different concentrations of lycopene (1, 5, and 10 µM), solubilized either in tetrahydrofuran (THF) or micelles as solvents. Incubation of all three cell types led to a concentration- and time-dependent increase in cellular lycopene content. Both vehicles tested, THF and micelles, proved equally effective in the delivery of lycopene to cells. A marked difference in the amount of lycopene incorporated was observed among the various cell types. Compared with THP-1 cells, the uptake of lycopene using both solvents was higher in BAECs and RAT-1 fibroblasts for all concentrations and time points tested.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Epidemiological data indicate a beneficial effect for consumption of tomato products in the prevention of cardiovascular diseases. Only limited data are available on the cellular uptake of lycopene in vascular cells. Lycopene was successfully delivered to different cells relevant for the cardiovascular system. These results represent an important prerequisite for the study of molecular and cellular mechanisms by which lycopene may exert its beneficial effects on the cardiovascular system.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01145.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63565293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-02-19DOI: 10.1111/j.1745-4522.2009.01130.x
V. VAN HOED, N. DE CLERCQ, C. ECHIM, M. ANDJELKOVIC, E. LEBER, K. DEWETTINCK, R. VERHÉ
ABSTRACT
Selected berry seed oils from blackberry, blueberry, cranberry, strawberry, red raspberry and kiwi were characterized for their quality and nutritional characteristics. These oils are by-products of berry juice production that have only recently gained commercial interest. Free fatty acid content was below 1.6% for all examined oil samples. Peroxide value ranged between 0.6 and 44 mg O2/kg oil for blackberry and kiwi seed oils, respectively, and p-anisidine value varied from 6 in cranberry to 23 in strawberry. Linolenic acid content ranged from 17.53% in blackberry seed oil to 57.60% in kiwi seed oil. The oxidative stability of all oils was rather low (0.17 h for kiwi to 8.4 h for blackberry at 97.8C). Phytosterol contents ranged between 403 and 692 mg/100 g for blackberry and cranberry, respectively. The content of tocols (tocopherol + tocotrienol) varied from 34.4 for kiwi to 2,133 mg/kg for red raspberry seed oils.
PRACTICAL APPLICATIONS
A waste stream of fruit processing is used to extract the oil from berry seeds. Such oils are particularly rich in essential fatty acids (with a favorable low n-6/n-3 ratio) and antioxidants. They are incorporated in cosmetic preparations such as hand and body creams, and shampoos. Their composition is also interesting from a nutritional point of view. As the commercial interest is growing, chemical studies are necessary to elucidate the composition, activity and stability of different berry seed oils.
{"title":"BERRY SEEDS: A SOURCE OF SPECIALTY OILS WITH HIGH CONTENT OF BIOACTIVES AND NUTRITIONAL VALUE","authors":"V. VAN HOED, N. DE CLERCQ, C. ECHIM, M. ANDJELKOVIC, E. LEBER, K. DEWETTINCK, R. VERHÉ","doi":"10.1111/j.1745-4522.2009.01130.x","DOIUrl":"10.1111/j.1745-4522.2009.01130.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Selected berry seed oils from blackberry, blueberry, cranberry, strawberry, red raspberry and kiwi were characterized for their quality and nutritional characteristics. These oils are by-products of berry juice production that have only recently gained commercial interest. Free fatty acid content was below 1.6% for all examined oil samples. Peroxide value ranged between 0.6 and 44 mg O<sub>2</sub>/kg oil for blackberry and kiwi seed oils, respectively, and</i> p<i>-anisidine value varied from 6 in cranberry to 23 in strawberry. Linolenic acid content ranged from 17.53% in blackberry seed oil to 57.60% in kiwi seed oil. The oxidative stability of all oils was rather low (0.17 h for kiwi to 8.4 h for blackberry at 97.8C). Phytosterol contents ranged between 403 and 692 mg/100 g for blackberry and cranberry, respectively. The content of tocols (tocopherol</i> + <i>tocotrienol) varied from 34.4 for kiwi to 2,133 mg/kg for red raspberry seed oils.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>A waste stream of fruit processing is used to extract the oil from berry seeds. Such oils are particularly rich in essential fatty acids (with a favorable low n-6/n-3 ratio) and antioxidants. They are incorporated in cosmetic preparations such as hand and body creams, and shampoos. Their composition is also interesting from a nutritional point of view. As the commercial interest is growing, chemical studies are necessary to elucidate the composition, activity and stability of different berry seed oils.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01130.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63564775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A gradual retarding of lipid digestion and absorption might contribute to preventing the visceral fat accumulation and obesity in humans. The lipolysis rates of different triacylglycerols (TAGs) by pancreatic lipase in vitro were studied in order to find suitable components as a retardant for lipolysis. Several TAGs containing longer acyl chains than stearic acid or containing unsaturated fatty acids whose double bonds are located closely to the carboxyl group like petroserinic and γ-linolenic acid showed significantly lower lipolysis rates as compared with triolein (OOO) control. Most of these resistant TAGs contributed to decelerating the lipolysis rate when replaced at 10% into the OOO control. Especially, 1-behenoyl-2,3-dioleoyl-rac-glycerol (BOO) was comprehensively thought to be a suitable TAG as a digestive retardant among them. Each 10% replacement of edible oils by BOO gave 15% to 30% reduction of the lipolysis rate in vitro. Resistant TAGs that are able to melt or solubilize into liquid oil at body temperature have some potential as a digestive retardant for lipids.
PRACTICAL APPLICATIONS
Visceral fat accumulation and obesity in humans are thought to be correlated with the rate of lipid digestion and absorption. The entire rate of lipid absorption is controlled by their partial replacement with some digestive retardant. Resistant triacylglycerols may have some potential as a digestive retardant of lipids. Results of the present study propose a new fat system for foods with antiobesity effect.
{"title":"SCREENING OF RESISTANT TRIACYLGLYCEROLS TO THE PANCREATIC LIPASE AND THEIR POTENTIALITIES AS A DIGESTIVE RETARDANT","authors":"TOSHIHARU ARISHIMA, NOBUHIKO TACHIBANA, MAKIKO KOJIMA, KIYOHARU TAKAMATSU, KATSUMI IMAIZUMI","doi":"10.1111/j.1745-4522.2009.01133.x","DOIUrl":"10.1111/j.1745-4522.2009.01133.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>A gradual retarding of lipid digestion and absorption might contribute to preventing the visceral fat accumulation and obesity in humans. The lipolysis rates of different triacylglycerols (TAGs) by pancreatic lipase</i> in vitro <i>were studied in order to find suitable components as a retardant for lipolysis. Several TAGs containing longer acyl chains than stearic acid or containing unsaturated fatty acids whose double bonds are located closely to the carboxyl group like petroserinic and γ-linolenic acid showed significantly lower lipolysis rates as compared with triolein (OOO) control. Most of these resistant TAGs contributed to decelerating the lipolysis rate when replaced at 10% into the OOO control. Especially, 1-behenoyl-2,3-dioleoyl-</i>rac<i>-glycerol (BOO) was comprehensively thought to be a suitable TAG as a digestive retardant among them. Each 10% replacement of edible oils by BOO gave 15% to 30% reduction of the lipolysis rate</i> in vitro<i>. Resistant TAGs that are able to melt or solubilize into liquid oil at body temperature have some potential as a digestive retardant for lipids.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Visceral fat accumulation and obesity in humans are thought to be correlated with the rate of lipid digestion and absorption. The entire rate of lipid absorption is controlled by their partial replacement with some digestive retardant. Resistant triacylglycerols may have some potential as a digestive retardant of lipids. Results of the present study propose a new fat system for foods with antiobesity effect.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01133.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63564841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-02-19DOI: 10.1111/j.1745-4522.2009.01128.x
G. GAMBACORTA, M. SINIGAGLIA, A. SCHENA, A. BAIANO, C. LAMACCHIA, S. PATI, E. LA NOTTE
ABSTRACT
A study on lipolysis of Southern Italy short-ripened dry-cured sausages manufactured without and with starters was carried out. Samples were submitted to microbiological and chemical analysis during drying and ripening, whereas sensory analysis was performed at the end of the ripening. The starters, addition caused the inhibition of the Enterobacteriaceae. An increase of free fatty acids (FFAs) and diacylglycerols (DAGs) during the ripening was observed. The addition of starter did not affect FFA and DAG release. The DAG profile including 1,2- and 1,3-isomers was analyzed for the first time in dry-cured sausages. Oleic, linoleic and palmitic acids, and 1,2-, 1,3-palmitinolein, 1,2- and 1,3-diolein were the most abundant compounds in FFA and DAG, respectively. A decrease in the concentration of some 1,2-DAG and an increase of 1,3-OO and 1,3-OP species during the process was observed. Finally, the addition of starter cultures slightly influenced the sensory properties of sausages.
PRACTICAL APPLICATIONS
In this work the development and application of an analytical method for the determination of free fatty acids (FFAs) and diacylglycerols (DAGs) in sausages was achieved. DAGs are intermediate products of hydrolysis and also constitute a substrate for hydrolysis reactions leading to FFA release. These latter compounds undergo a series of reactions leading to the formation of low-molecular-weight products responsible for sensory properties of dry-cured sausages. Therefore, the assessment of these lipolysis products is a useful tool for evaluating the ripening process of dry-cured sausages.
{"title":"CHANGES IN FREE FATTY ACID AND DIACYLGLYCEROL COMPOUNDS IN SHORT-RIPENING DRY-CURED SAUSAGE","authors":"G. GAMBACORTA, M. SINIGAGLIA, A. SCHENA, A. BAIANO, C. LAMACCHIA, S. PATI, E. LA NOTTE","doi":"10.1111/j.1745-4522.2009.01128.x","DOIUrl":"10.1111/j.1745-4522.2009.01128.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>A study on lipolysis of Southern Italy short-ripened dry-cured sausages manufactured without and with starters was carried out. Samples were submitted to microbiological and chemical analysis during drying and ripening, whereas sensory analysis was performed at the end of the ripening. The starters, addition caused the inhibition of the</i> Enterobacteriaceae. <i>An increase of free fatty acids (FFAs) and diacylglycerols (DAGs) during the ripening was observed. The addition of starter did not affect FFA and DAG release. The DAG profile including 1,2- and 1,3-isomers was analyzed for the first time in dry-cured sausages. Oleic, linoleic and palmitic acids, and 1,2-, 1,3-palmitinolein, 1,2- and 1,3-diolein were the most abundant compounds in FFA and DAG, respectively. A decrease in the concentration of some 1,2-DAG and an increase of 1,3-OO and 1,3-OP species during the process was observed. Finally, the addition of starter cultures slightly influenced the sensory properties of sausages.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>In this work the development and application of an analytical method for the determination of free fatty acids (FFAs) and diacylglycerols (DAGs) in sausages was achieved. DAGs are intermediate products of hydrolysis and also constitute a substrate for hydrolysis reactions leading to FFA release. These latter compounds undergo a series of reactions leading to the formation of low-molecular-weight products responsible for sensory properties of dry-cured sausages. Therefore, the assessment of these lipolysis products is a useful tool for evaluating the ripening process of dry-cured sausages.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01128.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63564644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-02-19DOI: 10.1111/j.1745-4522.2009.01129.x
V.V. PADMA, C.S.S. DEVI
ABSTRACT
Epidemiological studies indicate a low incidence of cardiac diseases in populations that consume large amounts of seafood. This effect is attributed to the presence of n-3 polyunsaturated fatty acids in fish oil. In this study, the protective effect of fish oil against isoproterenol induced changes in histopathology; hematology and lysosomal membrane integrity were evaluated in male wistar rats. The rats were fed fish oil for 45 days prior to isoproterenol treatment. The animals were sacrificed by cervical decapitation method 12 h after the second injection of isoproterenol hydrochloride. The results suggest that pretreatment with fish oil prevented isoproterenol-induced hematological changes. Lysosomal membrane integrity was also protected in fish oil pretreated rats, as indicated by significantly lowered activities of lysosomal hydrolases in serum and concomitant increase in their activity in the lysosomal fraction of heart. The histopathology studies further confirmed the cardioprotective effect of fish oil.
PRACTICAL APPLICATIONS
Fish oil consumption offers cardioprotection by its ability to modulate biological membranes. This study demonstrates the ability of fish oil to modulate hematological parameters and maintain lysosomal membrane integrity, thereby offering multifaceted protection to the heart. Hence, fish oils, especially those of marine origin, have excellent potential as cardioprotective agents.
{"title":"CARDIOPROTECTIVE EFFECT OF FISH OIL ON ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN RATS","authors":"V.V. PADMA, C.S.S. DEVI","doi":"10.1111/j.1745-4522.2009.01129.x","DOIUrl":"10.1111/j.1745-4522.2009.01129.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Epidemiological studies indicate a low incidence of cardiac diseases in populations that consume large amounts of seafood. This effect is attributed to the presence of n-3 polyunsaturated fatty acids in fish oil. In this study, the protective effect of fish oil against isoproterenol induced changes in histopathology; hematology and lysosomal membrane integrity were evaluated in male wistar rats. The rats were fed fish oil for 45 days prior to isoproterenol treatment. The animals were sacrificed by cervical decapitation method 12 h after the second injection of isoproterenol hydrochloride. The results suggest that pretreatment with fish oil prevented isoproterenol-induced hematological changes. Lysosomal membrane integrity was also protected in fish oil pretreated rats, as indicated by significantly lowered activities of lysosomal hydrolases in serum and concomitant increase in their activity in the lysosomal fraction of heart. The histopathology studies further confirmed the cardioprotective effect of fish oil.</i> </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Fish oil consumption offers cardioprotection by its ability to modulate biological membranes. This study demonstrates the ability of fish oil to modulate hematological parameters and maintain lysosomal membrane integrity, thereby offering multifaceted protection to the heart. Hence, fish oils, especially those of marine origin, have excellent potential as cardioprotective agents.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2009.01129.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63564706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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