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EXTRACTION OF COCOA BUTTER BY SUPERCRITICAL CARBON DIOXIDE: OPTIMIZATION OF OPERATING CONDITIONS AND EFFECT OF PARTICLE SIZE 超临界二氧化碳萃取可可脂:操作条件及粒径影响的优化
Pub Date : 2008-04-23 DOI: 10.1111/j.1745-4522.2008.00119.x
TENG-JU TAN, SELAMAT JINAP, ASEP EDI KUSNADI, NAZIMAH SHEIKH ABDUL HAMID

ABSTRACT

The optimum operating conditions for the extraction of cocoa butter from cocoa liquor using supercritical carbon dioxide and the effect of sample particle size on cocoa butter extraction under optimized operating conditions were investigated. The optimization was conducted at 10–45 MPa and 35–75C, with extraction times of 1–12 h by response surface methodology. The effect of particle size was studied using cocoa liquor, ground cocoa nibs and crushed cocoa nibs with particle sizes of approximately 74 µm, 0.85–1 mm and 4–6 mm, respectively. The yield was analyzed for total fat content by gravimetric method and triacylglycerol (TAG) profile by high-performance liquid chromatography. The results showed higher yield of cocoa butter with higher values of pressure, temperature and extraction time. The optimum conditions for cocoa butter extraction were 45 MPa, 75C and 12 h. The smaller particle size produced a higher yield of cocoa butter. 1,3-Dipalmitoyl-2-oleoyl-glycerol (POP), 1-palmitoyl-2-oleoyl-3-stearoyl-glycerol (POS) and 1,3-distearoyl-2-oleoyl-glycerol (SOS) were the major TAGs present in the extracted cocoa butter, with POS being the highest (>30%) for all treatments studied.

PRACTICAL APPLICATIONS

The application of supercritical carbon dioxide extraction offers a pollution-free technology that will gain wide acceptance globally because of increasingly stringent environmental regulations. The results from the study would assist the industry in the production of cocoa butter that is safe for consumption and free from contaminants, in contrast to those produced by organic solvent and mechanical expression.

摘要研究了超临界二氧化碳萃取可可液中可可脂的最佳工艺条件,以及在最佳工艺条件下样品粒度对可可脂提取率的影响。响应面法优化条件为:10-45 MPa, 35 - 75℃,提取时间1-12 h。采用粒径约为74 μ m、0.85-1 mm和4-6 mm的可可液、磨碎的可可粒和粉碎的可可粒,研究了粒径的影响。用重量法分析总脂肪含量,用高效液相色谱法分析三酰甘油(TAG)谱。结果表明,压力、温度和提取时间越高,可可脂得率越高。可可脂提取的最佳条件为45 MPa、75℃、12 h。颗粒越小,可可脂的产量越高。1,3-二棕榈酰-2-油酰甘油(POP)、1-棕榈酰-2-油酰-3-硬脂酰甘油(POS)和1,3-二硬脂酰-2-油酰甘油(SOS)是提取可可脂中主要的标签,其中POS在所有处理中含量最高(30%)。超临界二氧化碳萃取技术的应用提供了一种无污染的技术,由于日益严格的环境法规,该技术将在全球范围内得到广泛的接受。这项研究的结果将有助于该行业生产可安全消费且不受污染的可可脂,而不是那些由有机溶剂和机械表达生产的可可脂。
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引用次数: 10
PHOSPHATIDYLCHOLINE LEVELS AND THEIR FATTY ACID COMPOSITIONS IN SQUID EGG: A COMPARISON STUDY WITH POLLACK ROE AND STURGEON CAVIAR 鱿鱼蛋中磷脂酰胆碱含量及其脂肪酸组成:与明太鱼籽和鲟鱼鱼子酱的比较研究
Pub Date : 2008-04-23 DOI: 10.1111/j.1745-4522.2008.00114.x
QI WANG, CHANG-HU XUE, ZHAO-JIE LI, JIE XU

ABSTRACT

The content of total lipids (TLs), composition of phospholipids (PLs) and main fatty acids of phosphatidylcholine (PC) were determined in egg of squid Sthenoteuthis oualaniensis, roe of Alaska pollack Theragra chatcogramma and caviar of sturgeon Acipenser gueldenstaedti. The PL content of squid egg (72.0 ± 3.2% of TL) was higher than the other two kinds of fish eggs. A high-performance liquid chromatography/evaporate light-scattering detector method was used for qualitative and quantitative analyses of the PLs. The PC content of squid egg (67.50 ± 0.55% of PL) was the highest. Docosahexaenoic acid (22:6n-3, DHA) percentage of PC in squid egg (40.0 ± 0.4 mol %) was much higher than others. The results indicated that squid egg could be used as a new source of PC rich in DHA.

PRACTICAL APPLICATIONS

This study compares the phospholipids in egg from the squid Sthenoteuthis oualaniensis with two kinds of known fish eggs. The squid source was abundant in phospholipids, but it is not fully utilized because of the low-edible value of the meat. The analysis of the phospholipid classes was carried out by using a high-performance liquid chromatography/evaporative light-scattering detector. The method has been used in the dairy industry, but is not so common in fish product evaluation. Our results showed that it is quick and sensitive in detecting the fish and shellfish egg phospholipids. Squid egg contained a large amount of phosphatidylcholine and was rich in docosahexaenoic acid, thus may serve as a novel functional lipid for health promotion. Therefore, squid egg may serve as a valuable good source of functional lipids.

摘要测定了鱿鱼(Sthenoteuthis oualaniensis)卵、阿拉斯加明太鱼(Theragra chatcogramma)卵和鲟鱼(Acipenser gueldenstaedti)鱼子酱中总脂(TLs)含量、磷脂(PLs)组成和磷脂酰胆碱(PC)主要脂肪酸的含量。鱿鱼卵的PL含量(TL的72.0±3.2%)高于其他两种鱼卵。采用高效液相色谱/蒸发光散射检测法对其进行定性和定量分析,鱿鱼蛋中PC含量最高(占PL的67.50±0.55%)。鱿鱼蛋中PC的二十二碳六烯酸(22:6n-3, DHA)含量为40.0±0.4 mol %。结果表明,鱿鱼蛋可作为富含DHA的PC的新来源。本研究比较了鱿鱼(Sthenoteuthis oualaniensis)和两种已知鱼卵的磷脂含量。鱿鱼源磷脂含量丰富,但由于鱿鱼肉的食用价值较低,磷脂未得到充分利用。采用高效液相色谱/蒸发光散射检测器对磷脂类进行分析。该方法已用于乳制品行业,但在鱼类产品评估中并不常见。结果表明,该方法能快速、灵敏地检测鱼类和贝类的卵磷脂。鱿鱼蛋中含有大量的磷脂酰胆碱和丰富的二十二碳六烯酸,可作为一种新型的功能性脂质促进健康。因此,鱿鱼蛋可以作为一种有价值的功能性脂质的良好来源。
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引用次数: 20
DISTRIBUTION PROFILES OF TOCOPHEROLS AND FATTY ACIDS OF PHOSPHOLIPIDS IN ADZUKI BEANS (VIGNA ANGULARIS) 赤豆(vigna angularis)磷脂中生育酚和脂肪酸的分布特征
Pub Date : 2008-04-23 DOI: 10.1111/j.1745-4522.2008.00113.x
HIROMI YOSHIDA, NAOKO YOSHIDA, YUKA TOMIYAMA, MASAYUKI SAIKI, YOSHIYUKI MIZUSHINA

ABSTRACT

Extracted lipids from adzuki beans (Vigna angularis) were determined by high-performance liquid chromatography for tocopherol homologues. Fatty acid distributions of triacylglycerols (TAGs) and major phospholipids (PLs) were analyzed by gas chromatography, and their tocopherol contents were determined. δ-Tocopherol was present in highest concentration (77.6%), and γ-tocopherol in small amounts (22.4%). However, α- and β-tocopherols were not detected in adzuki beans. The major lipid components were PL (63.5%) and TAG (27.1%), while other components were also present in minor proportions (0.2–5.1%). The major PL components were phosphatidylcholine (45.3%), phosphatidylethanolamine (25.8%) and phosphatidylinositol (21.5%). The positional distribution of fatty acids in these PLs was evident in the beans: unsaturated fatty acids were predominantly located in the sn-2 position, while saturated fatty acids primary occupied the sn-1 position in these lipids.

PRACTICAL APPLICATIONS

This study describes the characteristics of lipid components, positional distribution of fatty acids in the major phospholipids as well as the tocopherol profiles of adzuki beans. These results were not similar to the positional fatty acid distributions in the oilseeds such as peanuts, soybeans and sesame seeds. The data obtained would be useful to both consumers and producers for manufacturing traditional adzuki confectionaries (wagashi) in Japan and elsewhere.

摘要采用高效液相色谱法对小豆中提取的脂质进行了生育酚同系物的测定。用气相色谱法分析了甘油三酯(TAGs)和主要磷脂(PLs)的脂肪酸分布,并测定了其生育酚含量。δ-生育酚含量最高(77.6%),γ-生育酚含量较低(22.4%)。赤豆中未检测到α-和β-生育酚。脂质成分主要为PL(63.5%)和TAG(27.1%),其他成分也有少量存在(0.2-5.1%)。主要成分为磷脂酰胆碱(45.3%)、磷脂酰乙醇胺(25.8%)和磷脂酰肌醇(21.5%)。脂肪酸在这些脂质中的位置分布很明显:不饱和脂肪酸主要位于sn-2位置,而饱和脂肪酸主要位于sn-1位置。本研究描述了赤豆的脂质成分的特征,脂肪酸在主要磷脂中的位置分布以及生育酚的分布。这些结果与花生、大豆和芝麻等油籽中脂肪酸的位置分布不相似。所获得的数据将对日本和其他地方的消费者和生产商生产传统红豆糖果(wagashi)都很有用。
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引用次数: 7
A STUDY OF ADLAY ON LOWERING SERUM AND LIVER LIPIDS IN HAMSTERS 延缓剂对仓鼠降血脂作用的研究
Pub Date : 2008-04-23 DOI: 10.1111/j.1745-4522.2008.00110.x
YULING LIN, CHINGMIN E. TSAI

ABSTRACT

The functional component of adlay responsible for lowering blood lipids was studied using an animal model. Forty male hamsters were randomly divided into four dietary groups, including control, whole grain adlay (WGA), oil-free adlay (OFA) and adlay oil (AO) groups. All groups were fed a high fat diet containing 11.8% oil and 0.2% cholesterol for 8 weeks. The results showed that the WGA, OFA and AO diets could decrease triacylglycerol (TAG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) concentrations in serum (P < 0.05). Specifically, all lipid levels were significantly lowered in the WGA and OFA groups as compared to the AO group (P < 0.05). Ingestion of WGA or OFA also reduced liver TAG and TC concentrations (P < 0.05). The data demonstrated that WGA, OFA and AO prolonged the lag phase of LDL oxidation (P < 0.05). Moreover, hamsters fed with WGA or OFA had lower activity of malic enzyme in liver than those fed with control or AO diet (P < 0.05). WGA, OFA and AO diets were also found to enhance the activity of glucose-6-phosphate dehydrogenase. Thus, the beneficial components of adlay were mainly in the OFA portion.

PRACTICAL APPLICATIONS

Adlay is one of the most common components used in Chinese herbal medicine for hypolipidemic effect and immunoregulation. Numerous studies had demonstrated that adlay can decrease serum total cholesterol, triacylglycerol and low-density lipoprotein cholesterol concentrations in animal models and human subjects. However, the shelf-life of processed adlay products is relatively short due to the easy oxidation of its oil. According to the results of this study, it seems reasonable to remove the oil component of adlay grains to prevent its peroxidation and the development of off-flavor in products while retaining most of the hypolipidemic effects of adlay.

摘要:采用动物模型研究了延迟的降血脂功能成分。将40只雄性仓鼠随机分为4个饲粮组:对照组、全谷物饲粮组(WGA)、无油饲粮组(OFA)和无油饲粮组(AO)。各组均饲喂油含量11.8%、胆固醇含量0.2%的高脂日粮,为期8周。结果表明,WGA、OFA和AO饲粮均可降低血清中甘油三酯(TAG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)浓度(P < 0.05)。具体而言,与AO组相比,WGA和OFA组的所有脂质水平均显著降低(P < 0.05)。摄入WGA或OFA也可降低肝脏TAG和TC浓度(P < 0.05)。数据显示,WGA、OFA和AO延长了LDL氧化滞后期(P < 0.05)。此外,饲喂WGA或OFA的仓鼠肝脏苹果酸酶活性低于对照组和AO组(P < 0.05)。WGA、OFA和AO均能提高葡萄糖-6-磷酸脱氢酶的活性。因此,延迟的有益成分主要在OFA部分。白藜芦醇是中药中最常用的降血脂和调节免疫功能的成分之一。大量研究表明,延迟可以降低动物模型和人类受试者的血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度。然而,由于其油容易氧化,加工后的延迟产品的保质期相对较短。根据本研究的结果,去除延迟谷物中的油脂成分似乎是合理的,以防止其过氧化和产品中异味的产生,同时保留延迟谷物的大部分降血脂作用。
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引用次数: 9
CHANGES OF OMEGA-3 FATTY ACID CONTENT AND LIPID COMPOSITION IN CANNED TUNA DURING 12-MONTH STORAGE 金枪鱼罐头贮存12个月期间ω -3脂肪酸含量及脂质组成的变化
Pub Date : 2008-04-23 DOI: 10.1111/j.1745-4522.2007.00109.x
SIRITHON SIRIAMORNPUN, LIFENG YANG, JITTAWAN KUBOLA, DUO LI

ABSTRACT

The effect of storage time on the concentration and lipid and fatty acid compositions of canned tuna in brine was studied. Lipid and fatty acid were analyzed during 0, 3, 6, 9 and 12 months of storage at room temperature. Concentrations of lipids and total saturated fatty acids were significantly increased after storage for 9 months. The concentrations of total polyunsaturated fatty acids (PUFAs), total n-3 and n-6 PUFA were significantly stepwise decreased with increased storage time (P < 0.05); 22:6n-3 and 22:5n-3 started to decrease significantly at 6 months, and 20:5n-3 and 20:4n-6 decreased at 3 months (P < 0.05). The n-3/n-6 ratio significantly decreased from 3.8 at 0 month to 3.2 at 9 months (P < 0.05). Cholesterol ester was significantly increased while the other four classes of lipids, namely, phospholipids, triacylglycerols, free fatty acids and sterols, showed an opposite trend during 12 months of storage. Lipid oxidation measured by peroxide value and thiobarbituric acid value showed gradual increases with storage time. Thus, canned tuna in brine should not be stored more than 6 months with consideration of the stability of n-3 fatty acids.

PRACTICAL APPLICATIONS

Canned foods are significant dietary items, especially for bush walkers, travelers and armed service personnel. Canned fish, one of the important sources of long-chain n-3 polyunsaturated fatty acids (PUFAs), have an equivalent n-3 PUFA content as fresh fish. Long-chain PUFA in fresh fish is unstable and easy to oxidize. Appropriate process produced canned fish have a number of advantages such as odor, taste, shelf life, storage and carrying compared with fresh fish. We have investigated the lipid oxidation and fatty acid alteration sequentially during a 12-month storage because there has been no such data available in the literature. The data from the present study could provide useful information for industries, consumers and public health workers.

摘要:研究了腌制时间对金枪鱼罐头浓度及脂质和脂肪酸组成的影响。分别在0、3、6、9和12个月的室温下分析脂质和脂肪酸。贮藏9个月后,脂质和总饱和脂肪酸浓度显著升高。总多不饱和脂肪酸(PUFAs)、总n-3和n-6 PUFA浓度随贮藏时间的延长呈显著递减趋势(P < 0.05);22:6n-3和22:5n-3在6个月时开始显著下降,20:5n-3和20:4n-6在3个月时下降(P < 0.05)。n-3/n-6比值由0月龄时的3.8显著降低至9月龄时的3.2 (P < 0.05)。在12个月的贮藏期间,胆固醇酯含量显著升高,而磷脂、甘油三酯、游离脂肪酸和甾醇等4类脂质的含量则呈相反趋势。过氧化值和硫代巴比妥酸值测定的脂质氧化随储存时间的延长而逐渐增加。因此,考虑到n-3脂肪酸的稳定性,卤水金枪鱼罐头不宜保存超过6个月。罐头食品是重要的饮食项目,特别是对丛林步行者,旅行者和军人。罐头鱼是长链n-3多不饱和脂肪酸(PUFAs)的重要来源之一,其n-3 PUFA含量与鲜鱼相当。鲜鱼中的长链PUFA不稳定,容易氧化。适当工艺生产的鱼罐头与鲜鱼相比,在气味、口感、保质期、储存和携带等方面具有许多优点。由于文献中没有这样的数据,我们研究了在12个月的储存过程中脂质氧化和脂肪酸改变的顺序。本研究的数据可以为工业、消费者和公共卫生工作者提供有用的信息。
{"title":"CHANGES OF OMEGA-3 FATTY ACID CONTENT AND LIPID COMPOSITION IN CANNED TUNA DURING 12-MONTH STORAGE","authors":"SIRITHON SIRIAMORNPUN,&nbsp;LIFENG YANG,&nbsp;JITTAWAN KUBOLA,&nbsp;DUO LI","doi":"10.1111/j.1745-4522.2007.00109.x","DOIUrl":"10.1111/j.1745-4522.2007.00109.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The effect of storage time on the concentration and lipid and fatty acid compositions of canned tuna in brine was studied. Lipid and fatty acid were analyzed during 0, 3, 6, 9 and 12 months of storage at room temperature. Concentrations of lipids and total saturated fatty acids were significantly increased after storage for 9 months. The concentrations of total polyunsaturated fatty acids (PUFAs), total n-3 and n-6 PUFA were significantly stepwise decreased with increased storage time (</i>P &lt; <i>0.05); 22:6n-3 and 22:5n-3 started to decrease significantly at 6 months, and 20:5n-3 and 20:4n-6 decreased at 3 months (</i>P &lt; <i>0.05). The n-3/n-6 ratio significantly decreased from 3.8 at 0 month to 3.2 at 9 months (</i>P &lt; <i>0.05). Cholesterol ester was significantly increased while the other four classes of lipids, namely, phospholipids, triacylglycerols, free fatty acids and sterols, showed an opposite trend during 12 months of storage. Lipid oxidation measured by peroxide value and thiobarbituric acid value showed gradual increases with storage time. Thus, canned tuna in brine should not be stored more than 6 months with consideration of the stability of n-3 fatty acids.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Canned foods are significant dietary items, especially for bush walkers, travelers and armed service personnel. Canned fish, one of the important sources of long-chain n-3 polyunsaturated fatty acids (PUFAs), have an equivalent n-3 PUFA content as fresh fish. Long-chain PUFA in fresh fish is unstable and easy to oxidize. Appropriate process produced canned fish have a number of advantages such as odor, taste, shelf life, storage and carrying compared with fresh fish. We have investigated the lipid oxidation and fatty acid alteration sequentially during a 12-month storage because there has been no such data available in the literature. The data from the present study could provide useful information for industries, consumers and public health workers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":"15 2","pages":"164-175"},"PeriodicalIF":0.0,"publicationDate":"2008-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2007.00109.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63562046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
FATTY ACID COMPOSITIONS OF TURKISH SHORTENINGS WITH EMPHASIS ON TRANS FATTY ACIDS 土耳其起酥油的脂肪酸组成,重点是反式脂肪酸
Pub Date : 2008-04-23 DOI: 10.1111/j.1745-4522.2008.00117.x
BELGIN BASOL, MURAT TASAN

ABSTRACT

The fatty acid (FA ) composition and the trans FA (TFA) content of Turkish shortenings were determined by capillary gas–liquid chromatography. The saturated FA, monounsaturated FA and polyunsaturated FA contents were within the ranges of 27.4–48.8, 33.8–56.7 and 8.4–35.3% of total FA, respectively. The total TFA ranged from 2.7 to 23.9% of total FA. The total trans C18:1 acid was the major TFA in all the analyzed brands, representing 82.3–94.5% of total TFA. The total trans C18:2 acid varied from 0.2 to 2.9% of total FA, while the total trans C18:3 acid was only found at very low levels (0.1% of total FA) in the samples of two brands. It is clear from the results that partially hydrogenated vegetable oils with high TFA content are still the major raw materials used in the production of Turkish shortenings.

PRACTICAL APPLICATIONS

The fatty acid (FA) composition of the shortenings, with emphasis on trans FA (TFA), was studied. It is important to reduce the amounts of dietary TFA because of their deleterious effects on human health, mainly on the cardiovascular system. As demonstrated in the present investigation, nevertheless, partially hydrogenated vegetable oils with high TFA content are still the major raw materials used for shortening production. On the other hand, reducing the total TFA content in the shortenings leads to an increase in saturated FA content. It is important to consider the data obtained in the industrial applications in order to improve the FA profile of the shortenings.

摘要采用毛细管气液色谱法测定土耳其起酥油中脂肪酸(FA)组成和反式脂肪酸(TFA)含量。饱和FA、单不饱和FA和多不饱和FA含量分别占总FA的27.4% ~ 48.8%、33.8 ~ 56.7和8.4 ~ 35.3%。总总脂肪酸占总脂肪酸的比例在2.7 ~ 23.9%之间。在所有被分析的品牌中,总反式C18:1酸是主要的TFA,占总TFA的82.3-94.5%。总反式C18:2酸占总FA的比例从0.2到2.9%不等,而总反式C18:3酸在两个品牌的样品中只占非常低的水平(占总FA的0.1%)。从结果可以清楚地看出,TFA含量高的部分氢化植物油仍然是生产土耳其起酥油的主要原料。研究了起酥油的脂肪酸组成,重点研究了反式脂肪酸组成。由于TFA对人体健康,主要是心血管系统的有害影响,因此减少饮食中TFA的摄入量是很重要的。然而,正如本研究所表明的那样,具有高TFA含量的部分氢化植物油仍然是起酥油生产的主要原料。另一方面,降低起酥油中总TFA含量导致饱和FA含量增加。考虑工业应用中获得的数据,以改善缩短剂的FA分布是很重要的。
{"title":"FATTY ACID COMPOSITIONS OF TURKISH SHORTENINGS WITH EMPHASIS ON TRANS FATTY ACIDS","authors":"BELGIN BASOL,&nbsp;MURAT TASAN","doi":"10.1111/j.1745-4522.2008.00117.x","DOIUrl":"10.1111/j.1745-4522.2008.00117.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>The fatty acid (FA ) composition and the </i>trans<i> FA (TFA) content of Turkish shortenings were determined by capillary gas–liquid chromatography. The saturated FA, monounsaturated FA and polyunsaturated FA contents were within the ranges of 27.4–48.8, 33.8–56.7 and 8.4–35.3% of total FA, respectively. The total TFA ranged from 2.7 to 23.9% of total FA. The total</i> trans<i> C18:1 acid was the major TFA in all the analyzed brands, representing 82.3–94.5% of total TFA. The total </i>trans<i> C18:2 acid varied from 0.2 to 2.9% of total FA, while the total </i>trans<i> C18:3 acid was only found at very low levels (0.1% of total FA) in the samples of two brands. It is clear from the results that partially hydrogenated vegetable oils with high TFA content are still the major raw materials used in the production of Turkish shortenings.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>The fatty acid (FA) composition of the shortenings, with emphasis on <i>trans</i> FA (TFA), was studied. It is important to reduce the amounts of dietary TFA because of their deleterious effects on human health, mainly on the cardiovascular system. As demonstrated in the present investigation, nevertheless, partially hydrogenated vegetable oils with high TFA content are still the major raw materials used for shortening production. On the other hand, reducing the total TFA content in the shortenings leads to an increase in saturated FA content. It is important to consider the data obtained in the industrial applications in order to improve the FA profile of the shortenings.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":"15 2","pages":"240-250"},"PeriodicalIF":0.0,"publicationDate":"2008-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2008.00117.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63562621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
SHORT- AND LONG-TERM EFFECTS OF CORN OIL ON SERUM LIPID AND LIPOPROTEIN AND VISCERAL ABDOMINAL FAT PAD PARAMETERS OF RATS 玉米油对大鼠血脂、脂蛋白及内脏腹部脂肪垫参数的短期和长期影响
Pub Date : 2008-02-05 DOI: 10.1111/j.1745-4522.2007.00103.x
FARZAD ASADI, ALI SHAHRIARI, MALIHE POURKABIR, ROBIN MACLAREN

ABSTRACT

Obesity, in particular, abdominal obesity, is highly correlated with metabolic disorders and cardiovascular lesions in humans and animal models and may in fact play a role in their pathogenesis. The development of obesity, while partially genetic, is largely affected by environmental and lifestyle factors such as diet. The aim of the present study was to determine the short- and long-term effects of dietary corn oil on the abdominal fat pat morphology and serum lipid and lipoprotein profile. Forty male Wistar rats were randomly separated into two groups. The experimental group was fed a corn oil-rich diet (CRD), which consisted of 32.5% of kilocalories from corn oil and provided a total number of kcal/kg/day similar to the average western diet. The control group received regular chow (6.5% kilocalories from fat source). In each group, eight rats were sacrificed after 3 weeks and the remaining 12 rats in each group were sacrificed after 10 weeks. Adipocyte size, distribution and adipocyte number per gram of abdominal fat pad were determined. The total weight of the abdominal fat pad from the CRD rats was approximately twice the weight of the pad from the control rats at both time points (5.43 ± 0.93 g versus 11.6 ± 1.98 g after 3 weeks and 6.24 ± 1.38 g versus 11.18 ± 2.17 g after 10 weeks). There was a significant increase (P < 0.001) in adipocyte number per gram of fat and in the total triacylglycerol (TAG) content of the fat pad in the CRD group compared to the control at both short-term and long-term time points. However, the adipocyte size distribution showed a similar pattern in both the CRD and control groups. Serum TAG, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and very low-density lipoprotein cholesterol (VLDL-C) were also measured. In the long-term study, higher values of TAG (P = 0.015), TC (P < 0.001), low-density lipoprotein cholesterol (P < 0.001) and VLDL-C (P = 0.005) were seen in the CRD group in comparison with the control. These results suggest that the deleterious effects of a CRD are because of serum changes rather than adipocyte morphology changes.

PRACTICAL APPLICATION

Although further research is required, corn oil administration may be associated with the development of cardiovascular disorders.

肥胖,特别是腹部肥胖,在人类和动物模型中与代谢紊乱和心血管病变高度相关,实际上可能在其发病机制中发挥作用。肥胖的发展虽然部分是遗传的,但在很大程度上受环境和生活方式因素(如饮食)的影响。本研究的目的是确定玉米油对腹部脂肪部形态和血清脂质和脂蛋白谱的短期和长期影响。40只雄性Wistar大鼠随机分为两组。试验组饲喂富含玉米油的饲粮(CRD),其中玉米油所提供的千卡含量为32.5%,总千卡/kg/d与普通西式饲粮相近。对照组给予常规食物(脂肪来源6.5%千卡)。3周后每组处死8只大鼠,10周后每组处死12只大鼠。测定脂肪细胞大小、分布及每克腹部脂肪垫脂肪细胞数量。在两个时间点,CRD大鼠腹部脂肪垫的总重量约为对照组大鼠腹部脂肪垫重量的两倍(3周后为5.43±0.93 g, 3周后为11.6±1.98 g; 10周后为6.24±1.38 g, 10周后为11.18±2.17 g)。在短期和长期时间点,与对照组相比,CRD组每克脂肪的脂肪细胞数量和脂肪垫的总三酰甘油(TAG)含量均显著增加(P < 0.001)。然而,脂肪细胞大小分布在CRD组和对照组中表现出相似的模式。测定血清TAG、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和极低密度脂蛋白胆固醇(VLDL-C)。在长期研究中,CRD组TAG (P = 0.015)、TC (P < 0.001)、低密度脂蛋白胆固醇(P < 0.001)、VLDL-C (P = 0.005)均高于对照组。这些结果表明,CRD的有害作用是由于血清的改变而不是脂肪细胞形态的改变。虽然还需要进一步的研究,但玉米油摄入可能与心血管疾病的发生有关。
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引用次数: 7
FATTY ACIDS, TOCOPHEROLS, STEROLS, PHENOLIC PROFILES AND OXIDATIVE STABILITY OF CUCUMIS MELO VAR. AGRESTIS OIL 甜瓜油的脂肪酸、生育酚、甾醇、酚类及氧化稳定性
Pub Date : 2008-02-05 DOI: 10.1111/j.1745-4522.2007.00102.x
ABDALBASIT MARIOD, BERTRAND MATTHÄUS

ABSTRACT

Oil extracted from the seeds of Cucumis melo var. agrestis, collected from Ghibaish (sandy soil) and Gezira (heavy clay soil) provinces in Sudan, was studied in terms of the profile of fatty acids, tocopherols and sterols as well as phenolic compounds and oxidative stability by Rancimat (Metrohm AG, Herisau, Switzerland). The predominant fatty acid was 18:2n-6, representing 61.3 and 61.4% for Ghibaish and Gezira samples, respectively. There were no variations among the contents of 16:0, 18:0, 18:1n-9 and 18:2n-6 between the two samples. γ-Tocopherol was the predominant tocopherol in both samples, representing 80.7 and 77.6% of the total tocopherols, respectively, followed by α-tocopherol at 18 and 21%, respectively. Total sterol content was 3,879.0 and 3,785.0 mg/kg for Ghibaish and Gezira samples, respectively. The main sterol of the two oils was β-sitosterol. The two samples showed an oxidative stability of 5.9 and 5.7 h, respectively. The content of total phenolic compounds was determined in each sample according to the Folin-Ciocalteau method as 33.0 and 31.9 mg/g of dry product, respectively. The phenolic compounds of the oil were separated using a solid phase extraction. The fractions were studied by high-performance liquid chromatography with diode-array detection, which revealed four peaks in the two samples. The major components were catechin, vanillic acid, sinapic acid and callistephin. There was little variability according to location between the two samples.

PRACTICAL APPLICATIONS

Two samples from dried seeds of Cucumis melo var. agrestis were collected. Seeds were crushed and ground using a grinding mill; the oil was extracted from the ground seeds by extraction with petroleum ether in a Soxhlet apparatus, and the physicochemical properties of C. melo var. agrestis oil were determined. The fatty acid and sterol composition were investigated using gas chromatography; the different tocopherol homologs were identified by high-performance liquid chromatography (HPLC), and the oil oxidative stability was studied by Rancimat (Metrohm AG, Herisau, Switzerland) apparatus. The phenolic compounds were extracted from the obtained oil and then identified by HPLC with diode-array detection.

摘要:采用ranimat(瑞士Herisau Metrohm AG)对采自苏丹Ghibaish(沙质土壤)省和Gezira(重粘土土壤)省的甜瓜(Cucumis melo var. agrestis)籽油的脂肪酸、生育酚、甾醇、酚类化合物和氧化稳定性进行了研究。主要脂肪酸为18:2n-6,分别占吉巴什和Gezira样品的61.3%和61.4%。两种样品中16:0、18:0、18:1n-9和18:2n-6的含量无差异。γ-生育酚为主要的生育酚,分别占总生育酚的80.7%和77.6%,其次是α-生育酚,分别占总生育酚的18%和21%。Ghibaish和Gezira样品的总甾醇含量分别为3,879.0和3,785.0 mg/kg。两种油的主要甾醇为β-谷甾醇。两种样品的氧化稳定性分别为5.9和5.7 h。采用Folin-Ciocalteau法测定各样品中总酚类化合物的含量分别为33.0 mg/g和31.9 mg/g。采用固相萃取法分离油中的酚类化合物。用高效液相色谱二极管阵列检测对其进行了分析,发现两种样品中存在4个峰。主要成分为儿茶素、香草酸、辛酸和石蒜素。两个样本之间的位置差异很小。本文对甜瓜(Cucumis melo var. agrestis)的两种干燥种子进行了取样。种子被碾碎,用研磨机研磨;用索氏装置用石油醚萃取法从甜瓜碎籽中提取精油,测定了甜瓜油的理化性质。用气相色谱法分析了脂肪酸和甾醇的组成;采用高效液相色谱法(HPLC)鉴定不同的生育酚同源物,并采用ranimat (Metrohm AG, Herisau, Switzerland)仪器研究油的氧化稳定性。从得到的油中提取酚类化合物,用高效液相色谱二极管阵列检测进行鉴定。
{"title":"FATTY ACIDS, TOCOPHEROLS, STEROLS, PHENOLIC PROFILES AND OXIDATIVE STABILITY OF CUCUMIS MELO VAR. AGRESTIS OIL","authors":"ABDALBASIT MARIOD,&nbsp;BERTRAND MATTHÄUS","doi":"10.1111/j.1745-4522.2007.00102.x","DOIUrl":"10.1111/j.1745-4522.2007.00102.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p> <i>Oil extracted from the seeds of </i>Cucumis melo <i>var. </i>agrestis, <i>collected from Ghibaish (sandy soil) and Gezira (heavy clay soil) provinces in Sudan, was studied in terms of the profile of fatty acids, tocopherols and sterols as well as phenolic compounds and oxidative stability by Rancimat (Metrohm AG, Herisau, Switzerland). The predominant fatty acid was 18:2n-6, representing 61.3 and 61.4% for Ghibaish and Gezira samples, respectively. There were no variations among the contents of 16:0, 18:0, 18:1n-9 and 18:2n-6 between the two samples. γ-Tocopherol was the predominant tocopherol in both samples, representing 80.7 and 77.6% of the total tocopherols, respectively, followed by α-tocopherol at 18 and 21%, respectively. Total sterol content was 3,879.0 and 3,785.0 mg/kg for Ghibaish and Gezira samples, respectively. The main sterol of the two oils was β-sitosterol. The two samples showed an oxidative stability of 5.9 and 5.7 h, respectively. The content of total phenolic compounds was determined in each sample according to the Folin-Ciocalteau method as 33.0 and 31.9 mg/g of dry product, respectively. The phenolic compounds of the oil were separated using a solid phase extraction. The fractions were studied by high-performance liquid chromatography with diode-array detection, which revealed four peaks in the two samples. The major components were catechin, vanillic acid, sinapic acid and callistephin. There was little variability according to location between the two samples.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>Two samples from dried seeds of <i>Cucumis melo </i>var. <i>agrestis </i>were collected. Seeds were crushed and ground using a grinding mill; the oil was extracted from the ground seeds by extraction with petroleum ether in a Soxhlet apparatus, and the physicochemical properties of <i>C. melo </i>var. <i>agrestis </i>oil were determined. The fatty acid and sterol composition were investigated using gas chromatography; the different tocopherol homologs were identified by high-performance liquid chromatography (HPLC), and the oil oxidative stability was studied by Rancimat (Metrohm AG, Herisau, Switzerland) apparatus. The phenolic compounds were extracted from the obtained oil and then identified by HPLC with diode-array detection.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":"15 1","pages":"56-67"},"PeriodicalIF":0.0,"publicationDate":"2008-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2007.00102.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63560948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
PHYSICOCHEMICAL PROPERTIES OF CUCUMIS MELO VAR. INODORUS (HONEYDEW MELON) SEED AND SEED OIL 甜瓜籽及籽油的理化性质
Pub Date : 2008-02-05 DOI: 10.1111/j.1745-4522.2007.00101.x
N.A.M. YANTY, O.M. LAI, A. OSMAN, K. LONG, H.M. GHAZALI

ABSTRACT

Cucumis melo var. inodorus seeds were found to contain 4.5% moisture, 25.0% crude fat, 25.0% crude protein, 23.3% crude fiber, 2.4% ash and 19.8% carbohydrate. The iodine and saponification values, and unsaponifiable matter and free fatty acid contents of freshly extracted honeydew melon seed oil were 153.4 g I2/100 g oil, 210.2 mg KOH/g oil, 0.9 and 2.5%, respectively. The oil had a color index of 1.6Y + 0.4R, and had 10 fatty acids, of which 86.1% were unsaturated. Linoleic acid predominated with 69.0% followed by oleic acid (16.8%) and palmitic acid (8.4%). LLL (24.9%), OLL (21.5%), PLL (15.9%) and POL (12.4%) (where L, O, P and S denote linoleic, oleic, palmitic and stearic acids, respectively) were the major triacylglycerols present. The melting and crystallization temperatures were 5.12 and 59.01C, respectively. Electronic nose analysis showed the presence of more volatile compounds compared to refined sunflower oil, an oil rich also in linoleic acid.

PRACTICAL APPLICATIONS

In Malaysia, honeydew melon (Cucumis melo var. inodorus) seeds are normally treated as waste products. In this study, honeydew melon seed oil was found to have a potential to become a new edible oil source as it contained a level high in polyunsaturated fatty acids.

摘要/ ABSTRACT摘要:测定了甜瓜种子的水分含量为4.5%,粗脂肪含量为25.0%,粗蛋白质含量为25.0%,粗纤维含量为23.3%,灰分含量为2.4%,碳水化合物含量为19.8%。新鲜提取的哈密瓜籽油碘值为153.4 g /100 g油,皂化值为210.2 mg KOH/g油,不皂化物和游离脂肪酸含量分别为0.9和2.5%。该油的显色指数为1.6Y + 0.4R,含有10种脂肪酸,其中86.1%为不饱和脂肪酸。亚油酸含量最高,为69.0%,其次是油酸(16.8%)和棕榈酸(8.4%)。LLL(24.9%)、OLL(21.5%)、PLL(15.9%)和POL(12.4%)(其中L、O、P和S分别代表亚油酸、油酸、棕榈酸和硬脂酸)是主要的三酰基甘油。熔融温度为- 5.12℃,结晶温度为- 59.01℃。电子鼻分析显示,与精制葵花籽油(一种富含亚油酸的油)相比,这种油含有更多挥发性化合物。在马来西亚,蜜瓜(Cucumis melo var. inodorus)的种子通常被当作废物处理。本研究发现,哈密瓜籽油中多不饱和脂肪酸含量高,有潜力成为一种新的食用油来源。
{"title":"PHYSICOCHEMICAL PROPERTIES OF CUCUMIS MELO VAR. INODORUS (HONEYDEW MELON) SEED AND SEED OIL","authors":"N.A.M. YANTY,&nbsp;O.M. LAI,&nbsp;A. OSMAN,&nbsp;K. LONG,&nbsp;H.M. GHAZALI","doi":"10.1111/j.1745-4522.2007.00101.x","DOIUrl":"10.1111/j.1745-4522.2007.00101.x","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> ABSTRACT</h3>\u0000 \u0000 <p>Cucumis melo <i>var.</i> inodorus <i>seeds were found to contain 4.5% moisture, 25.0% crude fat, 25.0% crude protein, 23.3% crude fiber, 2.4% ash and 19.8% carbohydrate. The iodine and saponification values, and unsaponifiable matter and free fatty acid contents of freshly extracted honeydew melon seed oil were 153.4 g I<sub>2</sub>/100 g oil, 210.2 mg KOH/g oil, 0.9 and 2.5%, respectively. The oil had a color index of 1.6Y</i> + <i>0.4R, and had 10 fatty acids, of which 86.1% were unsaturated. Linoleic acid predominated with 69.0% followed by oleic acid (16.8%) and palmitic acid (8.4%). LLL (24.9%), OLL (21.5%), PLL (15.9%) and POL (12.4%) (where L, O, P and S denote linoleic, oleic, palmitic and stearic acids, respectively) were the major triacylglycerols present. The melting and crystallization temperatures were </i>−<i>5.12 and </i>−<i>59.01C, respectively. Electronic nose analysis showed the presence of more volatile compounds compared to refined sunflower oil, an oil rich also in linoleic acid.</i></p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PRACTICAL APPLICATIONS</h3>\u0000 \u0000 <p>In Malaysia, honeydew melon (<i>Cucumis melo</i> var. <i>inodorus</i>) seeds are normally treated as waste products. In this study, honeydew melon seed oil was found to have a potential to become a new edible oil source as it contained a level high in polyunsaturated fatty acids.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":"15 1","pages":"42-55"},"PeriodicalIF":0.0,"publicationDate":"2008-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2007.00101.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63559664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 66
RELATION BETWEEN SENSORY ASTRINGENCY OF EXTRACTS FROM SELECTED TANNIN-RICH FOODS AND THEIR ANTIOXIDANT ACTIVITY 选定的富含单宁的食物提取物的感官涩味与其抗氧化活性的关系
Pub Date : 2008-02-05 DOI: 10.1111/j.1745-4522.2007.00100.x
RYSZARD AMAROWICZ, AGNIESZKA TROSZYŃSKA, AGNIESZKA KOSIŃSKA, GRZEGORZ LAMPARSKI, FEREIDOON SHAHIDI

ABSTRACT

Extracts obtained from hazelnuts, walnuts, almonds, vetch seeds, buckwheat and buckwheat grits were examined for their sensory astringency and antioxidant properties. The sensory astringency was evaluated using the sensory scaling method and was expressed as sensation of astringency index (SAI). The antioxidant activity of extracts was analyzed using (1) total antioxidant activity (TAA), (2) the ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical, (3) bleaching of β-carotene and (4) reducing power. Human salivary protein (HSP) precipitation capacity of the extracts was assayed by spectrophotometric techniques. Statistically significant correlations (P ≤ 0.05) existed between SAI values and TAA, SAI value and reducing power, and SAI values and HSP precipitation. The presence of condensed tannins in the extracts was confirmed by UV spectral and high-performance liquid chromatography data.

PRACTICAL APPLICATIONS

The sensory astringency of the tannin-rich extracts is correlated with their antioxidant activity. This information and the regression equations reported in this study could help to prepare functional foods characterized by the high antioxidant properties that could meet consumer's acceptance. However, studies should continue to explain whether the masking of astringency of plant extract with hydrocolloids may render any undesirable effect on their antioxidant properties.

摘要研究了榛子、核桃、杏仁、紫薇籽、荞麦和荞麦粉的感官涩味和抗氧化性能。感官涩味用感觉标度法评价,用感觉涩味指数(SAI)表示。采用(1)总抗氧化活性(TAA)、(2)清除2,2-二苯基-1-苦味酰肼基自由基的能力、(3)β-胡萝卜素的漂白能力和(4)还原能力对提取物的抗氧化活性进行了分析。采用分光光度法测定提取液的人唾液蛋白(HSP)沉淀能力。SAI值与TAA、SAI值与还原功率、SAI值与HSP降水量之间存在显著相关(P≤0.05)。通过紫外光谱和高效液相色谱分析证实了栲胶中存在缩合单宁。富单宁提取物的感官涩味与其抗氧化活性有关。这些信息和本研究报告的回归方程有助于制备具有高抗氧化性能的功能性食品,并满足消费者的接受。然而,研究应该继续解释用水胶体掩盖植物提取物的涩味是否会对其抗氧化性能产生任何不良影响。
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引用次数: 7
期刊
Journal of Food Lipids
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