Pub Date : 2022-06-06DOI: 10.1080/15321819.2022.2080558
S. Mumu, A. Mustafa
ABSTRACT We investigated the effects of different concentrations of longevity spinach, Gynura procumbens, on the hematological parameters of acutely stressed Nile tilapia, Oreochromis niloticus, (average weight 461.81 ± 16.60 g and average length 28.71 ± 0.34 cm) and determined the best concentration. The fish were subjected to hormonal stress in this research. We fed the stress control group commercial feed with 0.01% hydrocortisone, a stress hormone (0.01% of fish body weight) without Gynura. All the treatment groups were supplemented with Gynura extracts (0.5 g/kg, 1.0 g/kg, and 1.5 g/kg of feed weight) in combination with hydrocortisone. We evaluated blood glucose, lysozyme activity, phagocytic capacity, hematocrit, spleen somatic index, and hepatosomatic index. During the acute stress period, G. procumbens has been shown to decrease the levels of blood glucose in 1.5 g/kg treatment group (49.60 mg/dl at Day 1; 53.75 mg/dl at Day 3) compared to stress control group (80.00 mg/dl at Day 1; 69.20 mg/dl at Day 3). Higher lysozyme activity observed in 1.5 g/kg Gynura treatment group (11.44 T/min at 540 nm) compared to control (7.85 T/min at 540 nm). The 1.5 g/kg treatment group maintained the homeostatic level of significant physiological parameters including phagocytic capacity, packed cell volume, and hepatosomatic index. These findings are promising for the development of new nutraceuticals for the aquaculture industry.
{"title":"Modulation of acute stress and immune response in tilapia, Oreochromis niloticus, using longevity spinach, Gynura procumbens extract, as nutraceuticals","authors":"S. Mumu, A. Mustafa","doi":"10.1080/15321819.2022.2080558","DOIUrl":"https://doi.org/10.1080/15321819.2022.2080558","url":null,"abstract":"ABSTRACT We investigated the effects of different concentrations of longevity spinach, Gynura procumbens, on the hematological parameters of acutely stressed Nile tilapia, Oreochromis niloticus, (average weight 461.81 ± 16.60 g and average length 28.71 ± 0.34 cm) and determined the best concentration. The fish were subjected to hormonal stress in this research. We fed the stress control group commercial feed with 0.01% hydrocortisone, a stress hormone (0.01% of fish body weight) without Gynura. All the treatment groups were supplemented with Gynura extracts (0.5 g/kg, 1.0 g/kg, and 1.5 g/kg of feed weight) in combination with hydrocortisone. We evaluated blood glucose, lysozyme activity, phagocytic capacity, hematocrit, spleen somatic index, and hepatosomatic index. During the acute stress period, G. procumbens has been shown to decrease the levels of blood glucose in 1.5 g/kg treatment group (49.60 mg/dl at Day 1; 53.75 mg/dl at Day 3) compared to stress control group (80.00 mg/dl at Day 1; 69.20 mg/dl at Day 3). Higher lysozyme activity observed in 1.5 g/kg Gynura treatment group (11.44 T/min at 540 nm) compared to control (7.85 T/min at 540 nm). The 1.5 g/kg treatment group maintained the homeostatic level of significant physiological parameters including phagocytic capacity, packed cell volume, and hepatosomatic index. These findings are promising for the development of new nutraceuticals for the aquaculture industry.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83197417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-23DOI: 10.1080/15321819.2022.2076109
Mahin Lashgari, Sanaz Keshavarz Shahbaz, A. Javadi, M. Sahmani, Maryam Khalaji, Amirhosein Maali, Farshad Foroughi
ABSTRACT Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), which suppresses T cell proliferation, is a promising candidate for the susceptibility genes to rheumatic arthritis diseases (RA). This study aims to examine the association between the polymorphisms of the CTLA-4 exon 1(+ 49) genes with RA in the Qazvin city of Iran population. The polymerase chain reaction of genomic DNA-restriction fragment length polymorphism (PCR-RFLP) was applied to genotype the CTLA-4 exon 1(+ 49) polymorphisms in 105 RA patients and 90 control subjects. Laboratory diagnostic tests were also measured for RA and control groups. Our results did not demonstrate a significant difference in allele and genotype frequencies of the CTLA-4 exon 1(+ 49) between RA patients and the control group (p < .0001). There was no significant difference in age at onset, CRP, RF value in patients with RA according to the CTLA-4 polymorphisms; just anti-CCP showed a significant difference. Our data declared that polymorphisms of CTLA-4 exon 1(+ 49) genes are not correlated with RA susceptibility and its clinical and paraclinical manifestations.
{"title":"Survey of the association between polymorphisms of CTLA-4 exon 1 49 A/G genes with rheumatoid arthritis in Iran","authors":"Mahin Lashgari, Sanaz Keshavarz Shahbaz, A. Javadi, M. Sahmani, Maryam Khalaji, Amirhosein Maali, Farshad Foroughi","doi":"10.1080/15321819.2022.2076109","DOIUrl":"https://doi.org/10.1080/15321819.2022.2076109","url":null,"abstract":"ABSTRACT Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), which suppresses T cell proliferation, is a promising candidate for the susceptibility genes to rheumatic arthritis diseases (RA). This study aims to examine the association between the polymorphisms of the CTLA-4 exon 1(+ 49) genes with RA in the Qazvin city of Iran population. The polymerase chain reaction of genomic DNA-restriction fragment length polymorphism (PCR-RFLP) was applied to genotype the CTLA-4 exon 1(+ 49) polymorphisms in 105 RA patients and 90 control subjects. Laboratory diagnostic tests were also measured for RA and control groups. Our results did not demonstrate a significant difference in allele and genotype frequencies of the CTLA-4 exon 1(+ 49) between RA patients and the control group (p < .0001). There was no significant difference in age at onset, CRP, RF value in patients with RA according to the CTLA-4 polymorphisms; just anti-CCP showed a significant difference. Our data declared that polymorphisms of CTLA-4 exon 1(+ 49) genes are not correlated with RA susceptibility and its clinical and paraclinical manifestations.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88917346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-04DOI: 10.1080/15321819.2022.2075624
M. Moscovitch-Lopatin
{"title":"Winner of the 2021 Journal of Immunoassay & Immunochemistry Early Career Research Prize","authors":"M. Moscovitch-Lopatin","doi":"10.1080/15321819.2022.2075624","DOIUrl":"https://doi.org/10.1080/15321819.2022.2075624","url":null,"abstract":"","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85106931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-02DOI: 10.1080/15321819.2022.2071127
Martin-Luther Oseni Okolo, C. Omatola, S. Samson, B. Idache
ABSTRACT Hepatitis B and enteric fever remain infectious diseases of major health concern and the etiologic agents share overlapping regions of high endemicity worldwide. This study determined the seroprevalence of hepatitis B and enteric fever among febrile patients in Ejule metropolis, North-central Nigeria. In a cross-sectional study, blood samples obtained from 200 consenting patients were screened for hepatitis B and enteric fever with standard diagnostics ABON hepatitis B surface antigen (HBsAg) and Widal agglutination tests, respectively. A structured questionnaire was used to collect socio-demographic and risk factor information. Of the 200 sera tested, 20 (10%) had HBV alone, 35 (17.5%) enteric fever while 6 (3%) had both HBV/enteric fever. Males recorded more HBV (12% vs 6.7%), enteric fever (20% vs 13.3%), and co-infection (3.2% vs 2.7%) than their counterpart females. Ages 18–30 years were more predisposed to single and dual infections compared to older categories. Although sex, ages as well as the participants’ occupation were not significantly linked to both infections (p > .05). The prevalence of enteric fever differed significantly with being married (p = .01). The illiterate had a higher prevalence of HBV, enteric fever, and dual infection (16.7%, 25%, and 8.3%) compared to higher educational levels. The study confirms the endemicity of hepatitis B and enteric fever in the area. Their concurrent rate highlights the need for clinicians to always consider the laboratory diagnosis of both diseases among febrile patients in endemic regions for proper management in events of co-morbidity. The high prevalence of both infections in connection with illiteracy shows the need to further intensify education of masses on the predisposing risk factors.
{"title":"Evidence of hepatitis B infection and co-infection with enteric fever among febrile patients in a primary health facility in Kogi State, Nigeria","authors":"Martin-Luther Oseni Okolo, C. Omatola, S. Samson, B. Idache","doi":"10.1080/15321819.2022.2071127","DOIUrl":"https://doi.org/10.1080/15321819.2022.2071127","url":null,"abstract":"ABSTRACT Hepatitis B and enteric fever remain infectious diseases of major health concern and the etiologic agents share overlapping regions of high endemicity worldwide. This study determined the seroprevalence of hepatitis B and enteric fever among febrile patients in Ejule metropolis, North-central Nigeria. In a cross-sectional study, blood samples obtained from 200 consenting patients were screened for hepatitis B and enteric fever with standard diagnostics ABON hepatitis B surface antigen (HBsAg) and Widal agglutination tests, respectively. A structured questionnaire was used to collect socio-demographic and risk factor information. Of the 200 sera tested, 20 (10%) had HBV alone, 35 (17.5%) enteric fever while 6 (3%) had both HBV/enteric fever. Males recorded more HBV (12% vs 6.7%), enteric fever (20% vs 13.3%), and co-infection (3.2% vs 2.7%) than their counterpart females. Ages 18–30 years were more predisposed to single and dual infections compared to older categories. Although sex, ages as well as the participants’ occupation were not significantly linked to both infections (p > .05). The prevalence of enteric fever differed significantly with being married (p = .01). The illiterate had a higher prevalence of HBV, enteric fever, and dual infection (16.7%, 25%, and 8.3%) compared to higher educational levels. The study confirms the endemicity of hepatitis B and enteric fever in the area. Their concurrent rate highlights the need for clinicians to always consider the laboratory diagnosis of both diseases among febrile patients in endemic regions for proper management in events of co-morbidity. The high prevalence of both infections in connection with illiteracy shows the need to further intensify education of masses on the predisposing risk factors.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86628284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-27DOI: 10.1080/15321819.2022.2070025
I. Nikas, G. Kazamias, M. Vrontaki, Aleka S Rapti, E. Mastorakis
ABSTRACT Medullary thyroid carcinoma (MTC) is a rare neuroendocrine malignancy that arises from the parafollicular cells (C cells) secreting calcitonin. This study summarizes our experience in the diagnosis of MTC with ultrasound-guided thyroid FNA, subsequently processed with liquid-based cytology (LBC) and immunocytochemistry (ICC). We searched our laboratory archives for thyroid FNA cases with an interpretation of positive or suspicious for MTC, during the period 2004–2018. A total of 20 cases (18 thyroid FNAs; two lymph node FNAs) were selected and included in this study. These displayed high cellularity and a discohesive pattern, with a few loose syncytial groups. There was some variation in the cell size and shape both across and within our cases. Most MTCs (n = 15) exhibited a predominant plasmacytoid/epithelioid cell morphology, whereas five of our cases showed a spindle cell pattern. Of interest, none of eight MTC microcarcinomas (≤1 cm) showed a spindle cell morphology. Amyloid was found in 11/20 cases (55%), while binucleation/multinucleation in 17/20 (85%), and nuclear pseudoinclusions in 3/20 MTC cases (15%). Nuclei exhibited a granular, “salt and pepper” chromatin in all cases. ICC was performed in 18/20 cases (90%). Calcitonin, CEA, TTF1, and Chromogranin were positive wherever applied, whereas thyroglobulin and CK19 were negative. In conclusion, ultrasound-guided thyroid FNA – processed with LBC and ICC – is a reliable modality to detect MTC preoperatively, facilitating the management of such patients.
{"title":"Medullary thyroid carcinoma diagnosed with liquid-based cytology and immunocytochemistry","authors":"I. Nikas, G. Kazamias, M. Vrontaki, Aleka S Rapti, E. Mastorakis","doi":"10.1080/15321819.2022.2070025","DOIUrl":"https://doi.org/10.1080/15321819.2022.2070025","url":null,"abstract":"ABSTRACT Medullary thyroid carcinoma (MTC) is a rare neuroendocrine malignancy that arises from the parafollicular cells (C cells) secreting calcitonin. This study summarizes our experience in the diagnosis of MTC with ultrasound-guided thyroid FNA, subsequently processed with liquid-based cytology (LBC) and immunocytochemistry (ICC). We searched our laboratory archives for thyroid FNA cases with an interpretation of positive or suspicious for MTC, during the period 2004–2018. A total of 20 cases (18 thyroid FNAs; two lymph node FNAs) were selected and included in this study. These displayed high cellularity and a discohesive pattern, with a few loose syncytial groups. There was some variation in the cell size and shape both across and within our cases. Most MTCs (n = 15) exhibited a predominant plasmacytoid/epithelioid cell morphology, whereas five of our cases showed a spindle cell pattern. Of interest, none of eight MTC microcarcinomas (≤1 cm) showed a spindle cell morphology. Amyloid was found in 11/20 cases (55%), while binucleation/multinucleation in 17/20 (85%), and nuclear pseudoinclusions in 3/20 MTC cases (15%). Nuclei exhibited a granular, “salt and pepper” chromatin in all cases. ICC was performed in 18/20 cases (90%). Calcitonin, CEA, TTF1, and Chromogranin were positive wherever applied, whereas thyroglobulin and CK19 were negative. In conclusion, ultrasound-guided thyroid FNA – processed with LBC and ICC – is a reliable modality to detect MTC preoperatively, facilitating the management of such patients.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72758239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.1080/15321819.2022.2048011
Vishva K V, Pradeep Gangwar, Jigarji Chaturji Thakor, M. Dinesh, M. Sahoo, R. Singh, S. Mahajan, S. Qureshi, Lahari Laddika, Nihar Ranjan Sahoo, Ujjawal K De
ABSTRACT Streptococcus suis is an emerging bacterial pathogen of huge economic impact to the swine industry worldwide. The information regarding the carrier status of S. suis in the slaughtered pigs along with its genetic characterization is not available in Indian pig population, which needs to be addressed for the therapeutic and preventive measures. In the present study, 563 palatine tonsils of apparently healthy slaughtered pigs were probed for the prevalence, and genetic characterization of S. suis and prevalence were found to be 15.45% and 32.68% by bacteriological and molecular methods, respectively. In 87 isolates recovered, 6 cps-types were detected showing the predominance of serotype 7 (24.13%) and 5 (18.39%), whereas 11 cps-types were detected in tonsillar DNA involving cps-types 9 (28.26%) and 7 (14.13%) as the major serotypes with arcA+/sly+/epf+/mrp− being the prevalent genotype. The histopathological changes with the immunodetection of S. suis antigen confirmed its persistence in asymptomatic carriers. Of 87 bacterial isolates, 7 isolates (serotypes 7 & 2) were pathogenic to Swiss albino mice showing the classical lesions of meningitis and septicemia. The presence of virulent serotypes of S. suis in healthy slaughtered pigs suggests a great health risk to the people engaged in piggery operations and in-contact pigs.
{"title":"Carrier status of Streptococcus suis in the palatine tonsils of apparently healthy slaughtered pigs of India","authors":"Vishva K V, Pradeep Gangwar, Jigarji Chaturji Thakor, M. Dinesh, M. Sahoo, R. Singh, S. Mahajan, S. Qureshi, Lahari Laddika, Nihar Ranjan Sahoo, Ujjawal K De","doi":"10.1080/15321819.2022.2048011","DOIUrl":"https://doi.org/10.1080/15321819.2022.2048011","url":null,"abstract":"ABSTRACT Streptococcus suis is an emerging bacterial pathogen of huge economic impact to the swine industry worldwide. The information regarding the carrier status of S. suis in the slaughtered pigs along with its genetic characterization is not available in Indian pig population, which needs to be addressed for the therapeutic and preventive measures. In the present study, 563 palatine tonsils of apparently healthy slaughtered pigs were probed for the prevalence, and genetic characterization of S. suis and prevalence were found to be 15.45% and 32.68% by bacteriological and molecular methods, respectively. In 87 isolates recovered, 6 cps-types were detected showing the predominance of serotype 7 (24.13%) and 5 (18.39%), whereas 11 cps-types were detected in tonsillar DNA involving cps-types 9 (28.26%) and 7 (14.13%) as the major serotypes with arcA+/sly+/epf+/mrp− being the prevalent genotype. The histopathological changes with the immunodetection of S. suis antigen confirmed its persistence in asymptomatic carriers. Of 87 bacterial isolates, 7 isolates (serotypes 7 & 2) were pathogenic to Swiss albino mice showing the classical lesions of meningitis and septicemia. The presence of virulent serotypes of S. suis in healthy slaughtered pigs suggests a great health risk to the people engaged in piggery operations and in-contact pigs.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87911847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-20DOI: 10.1080/15321819.2022.2053989
S. Ode, T. Jarikre, A. Jubril, B. Emikpe
ABSTRACT Toxoplasmosis is a zoonotic disease of economic importance found worldwide, and it is caused by Toxoplasma gondii, which affects a wide range of hosts. High prevalence of toxoplasmosis has been reported in rodents, and they are considered very important in the circulation and maintenance of the disease. However, epidemiologic studies of the disease in rodents are generally scarce in the Tropics. This study utilized the immunohistochemical (IHC) technique to detect Toxoplasma gondii in wild rats sampled from across the North Central Nigeria. The brain, intestine, diaphragm, lungs and kidney tissue samples from 227 wild rats (Zyzomys pedunculatus) were routinely processed for histopathology, out of which 86 were further selected for IHC detection of T. gondii antigens using the streptavidin-peroxidase method. The histologic lesions observed were mild to moderate in severity, including meningitis, focal gliosis, neuronal degeneration and necrosis, villous atrophy and denudation, enteritis, diaphragmatic myositis, broncho-interstitial pneumonia and interstitial nephritis. Toxoplasma gondii was detected in 82.6% of the selected samples showing various degrees of immunoreaction intensity. We conclude that IHC is a useful tool in the detection of T. gondii in wild rats, and lungs and kidney may be the organ of choice for the detection of T. gondii.
{"title":"Immunohistochemical detection and pathology of toxoplasmosis in Nigerian wild rats","authors":"S. Ode, T. Jarikre, A. Jubril, B. Emikpe","doi":"10.1080/15321819.2022.2053989","DOIUrl":"https://doi.org/10.1080/15321819.2022.2053989","url":null,"abstract":"ABSTRACT Toxoplasmosis is a zoonotic disease of economic importance found worldwide, and it is caused by Toxoplasma gondii, which affects a wide range of hosts. High prevalence of toxoplasmosis has been reported in rodents, and they are considered very important in the circulation and maintenance of the disease. However, epidemiologic studies of the disease in rodents are generally scarce in the Tropics. This study utilized the immunohistochemical (IHC) technique to detect Toxoplasma gondii in wild rats sampled from across the North Central Nigeria. The brain, intestine, diaphragm, lungs and kidney tissue samples from 227 wild rats (Zyzomys pedunculatus) were routinely processed for histopathology, out of which 86 were further selected for IHC detection of T. gondii antigens using the streptavidin-peroxidase method. The histologic lesions observed were mild to moderate in severity, including meningitis, focal gliosis, neuronal degeneration and necrosis, villous atrophy and denudation, enteritis, diaphragmatic myositis, broncho-interstitial pneumonia and interstitial nephritis. Toxoplasma gondii was detected in 82.6% of the selected samples showing various degrees of immunoreaction intensity. We conclude that IHC is a useful tool in the detection of T. gondii in wild rats, and lungs and kidney may be the organ of choice for the detection of T. gondii.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75957649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-18DOI: 10.1080/15321819.2022.2048010
K. Kalantar, N. Gholijani, E. Martinuzzi, S. Culina, D. Kabelitz, Z. Amirghofran
ABSTRACT Antigens derived from engulfed apoptotic bodies that are presented by dendritic cells can amplify Ag-specific T-cells. Accelerated co-cultured DC (acDC) strategy keeps lymphocytes in contact with differentiating DCs. Therefore, Ag-specific T-cell activation can occur during DC maturation. Our aim was to prepare DCs by acDC method and check the subsequent engulfment of the apoptotic body by acDC. We have proposed that this method could be feasible if we transfect the apoptotic bodies with the antigen. DCs were prepared using acDC method and their maturation markers were confirmed by flow cytometry. Ultraviolet was used for inducing apoptosis in the PBMCs and induction of apoptosis checked by propidium iodide and 7-aminoactinomycin D staining. Flow cytometry and immunohistochemistry were used for checking the uptake of apoptotic bodies by the DCs. The alloreactivity against apoptotic bodies was examined by enzyme-linked immunospot (ELISPOT) assay. Results showed that 40.4% of DCs could efficiently engulf the apoptotic bodies. The results indicated that acDC method is capable to isolate a high yield of DCs, and these cells could properly engulf the apoptotic bodies, more works should be performed to use this method for Ag discovery through delivering the Ag by apoptotic bodies into the DCs.
{"title":"Accelerated co-cultured dendritic cell (acDC) loaded with autologous apoptotic bodies might be a promising approach for antigen delivery","authors":"K. Kalantar, N. Gholijani, E. Martinuzzi, S. Culina, D. Kabelitz, Z. Amirghofran","doi":"10.1080/15321819.2022.2048010","DOIUrl":"https://doi.org/10.1080/15321819.2022.2048010","url":null,"abstract":"ABSTRACT Antigens derived from engulfed apoptotic bodies that are presented by dendritic cells can amplify Ag-specific T-cells. Accelerated co-cultured DC (acDC) strategy keeps lymphocytes in contact with differentiating DCs. Therefore, Ag-specific T-cell activation can occur during DC maturation. Our aim was to prepare DCs by acDC method and check the subsequent engulfment of the apoptotic body by acDC. We have proposed that this method could be feasible if we transfect the apoptotic bodies with the antigen. DCs were prepared using acDC method and their maturation markers were confirmed by flow cytometry. Ultraviolet was used for inducing apoptosis in the PBMCs and induction of apoptosis checked by propidium iodide and 7-aminoactinomycin D staining. Flow cytometry and immunohistochemistry were used for checking the uptake of apoptotic bodies by the DCs. The alloreactivity against apoptotic bodies was examined by enzyme-linked immunospot (ELISPOT) assay. Results showed that 40.4% of DCs could efficiently engulf the apoptotic bodies. The results indicated that acDC method is capable to isolate a high yield of DCs, and these cells could properly engulf the apoptotic bodies, more works should be performed to use this method for Ag discovery through delivering the Ag by apoptotic bodies into the DCs.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79879547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-14DOI: 10.1080/15321819.2022.2046601
O. Akpa, Oluwatoyin Babalola, A. Odetunde, A. Fagbamigbe, F. Fasola, B. Brown, N. Alamukii, C. Babalola, G. Odaibo, A. Falusi
ABSTRACT Transfusion transmissible infections (TTIs) such as Hepatitis B Virus (HBV), Hepatitis C Virus (HCV) and Human Immunodeficiency Virus (HIV) are among the most frequent complications in individuals with Sickle Cell Disease (SCD). We investigated factors associated with TTIs in SCD patients and controls in South-west Nigeria. A total of 2,034 participants with or without SCD were recruited in a matched case-control study. HIV, HBV and HCV infections were diagnosed using commercialy available ELISA kits (Biorad, Paris). Samples positive for HIV ELISA were further confirmed using Western blot. Data were analyzed using descriptive statistics, paired/independent t-test and logistic regression at p = .05. Proportion with HBV was higher among those with multiple sexual partners (12.7%), tattoo/body incision (11.8%), and sharing of sharp objects (7.3%), but HIV was only higher among participants with history of tattoo/body incision (1.5%). Prevalence of TTIs was similar among participants with or without transfusion. History of sharing sharp objects (adjusted odds ratios (aOR) = 1.72; 95%CI:1.11–2.66) and tattoo/body incision (aOR = 1.89; 95%CI:1.22–2.94) almost doubled the risk of HBV. TTIs are endemic in the studied area. Certain lifestyles predispose people to TTIs than having blood transfusion. Population-based intervention targeting lifestyle changes may reduce the risk of TTIs in the study area. Abbrveviations AA: Hemoglobin AA; AC: Hemoglobin AC; aOR: adjusted Odds Ratios; AS: Hemoglobin AS; CHOP: Children Outpatient; CI: Confidence Interval; EDTA: Ethylenediamine Tetraacetic Acid; GOP: General Outpatient; HBV: Hepatitis B Virus; HCV: Hepatitis C Virus; HIV: Human Immunodeficiency Virus; HPLC: High Performance Liquid Chromatography; IAMRAT: Advanced Medical Research & Training; IDU: Injection Drug Use; MOP: Medical Outpatient; SC: Hemoglobin SC; SCD: Sickle cell disease; SD: Standard Deviation; SF: Hemoglobin SF; SS: Hemoglobin SS; STDs: Sexually Transmitted Diseases; TTI: Transfusion transmissible infections; UCH: University College Hospital Ibadan; UI: University of Ibadan;
{"title":"Correlates of transfusion transmissible infections among patients with sickle cell disease in Nigeria: case-control study","authors":"O. Akpa, Oluwatoyin Babalola, A. Odetunde, A. Fagbamigbe, F. Fasola, B. Brown, N. Alamukii, C. Babalola, G. Odaibo, A. Falusi","doi":"10.1080/15321819.2022.2046601","DOIUrl":"https://doi.org/10.1080/15321819.2022.2046601","url":null,"abstract":"ABSTRACT Transfusion transmissible infections (TTIs) such as Hepatitis B Virus (HBV), Hepatitis C Virus (HCV) and Human Immunodeficiency Virus (HIV) are among the most frequent complications in individuals with Sickle Cell Disease (SCD). We investigated factors associated with TTIs in SCD patients and controls in South-west Nigeria. A total of 2,034 participants with or without SCD were recruited in a matched case-control study. HIV, HBV and HCV infections were diagnosed using commercialy available ELISA kits (Biorad, Paris). Samples positive for HIV ELISA were further confirmed using Western blot. Data were analyzed using descriptive statistics, paired/independent t-test and logistic regression at p = .05. Proportion with HBV was higher among those with multiple sexual partners (12.7%), tattoo/body incision (11.8%), and sharing of sharp objects (7.3%), but HIV was only higher among participants with history of tattoo/body incision (1.5%). Prevalence of TTIs was similar among participants with or without transfusion. History of sharing sharp objects (adjusted odds ratios (aOR) = 1.72; 95%CI:1.11–2.66) and tattoo/body incision (aOR = 1.89; 95%CI:1.22–2.94) almost doubled the risk of HBV. TTIs are endemic in the studied area. Certain lifestyles predispose people to TTIs than having blood transfusion. Population-based intervention targeting lifestyle changes may reduce the risk of TTIs in the study area. Abbrveviations AA: Hemoglobin AA; AC: Hemoglobin AC; aOR: adjusted Odds Ratios; AS: Hemoglobin AS; CHOP: Children Outpatient; CI: Confidence Interval; EDTA: Ethylenediamine Tetraacetic Acid; GOP: General Outpatient; HBV: Hepatitis B Virus; HCV: Hepatitis C Virus; HIV: Human Immunodeficiency Virus; HPLC: High Performance Liquid Chromatography; IAMRAT: Advanced Medical Research & Training; IDU: Injection Drug Use; MOP: Medical Outpatient; SC: Hemoglobin SC; SCD: Sickle cell disease; SD: Standard Deviation; SF: Hemoglobin SF; SS: Hemoglobin SS; STDs: Sexually Transmitted Diseases; TTI: Transfusion transmissible infections; UCH: University College Hospital Ibadan; UI: University of Ibadan;","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78487805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-07DOI: 10.1080/15321819.2022.2039184
T. Bharucha, Nicole Zitzmann, P. Newton, A. Dubot-Pérès, L. Turtle
To the Editor, Journal of Immunoassay and Immunochemistry 21 January 2022 Re: Sero-evidence of silent Japanese Encephalitis Virus infection among inhabitants of Ilorin, North-central Nigeria: a call for active surveillance https://www.tandfonline.com/doi/abs/10.1080/15321819.2021.1993897 We read with interest the recent article published in the Journal of Immunoassay and Immunochemistry reporting Japanese encephalitis virus (JEV) serological testing in Nigeria. There is no doubt that there are ongoing changes in the geographical distribution of flaviviruses, and surveillance for the relevant disease syndromes would be helpful. To our knowledge, serological testing of this kind has not been performed before in Nigeria. JEV RNA was apparently detected in a patient with confirmed yellow fever in Angola; however, acute JEV infection was not corroborated by conventional IgM ELISA or seroneutralisation. As the authors discuss in the article, there is high cross-reactivity between flaviviruses, and very significant false positives with ELISA tests. We urge caution in the interpretation of the results. Anti-JEV IgG results cannot be interpreted alone, they require to be reported alongside testing for other endemic flaviviruses, most importantly anti-dengue virus and yellow fever virus IgG, and flavivirus vaccination history. Yellow fever is reported in Nigeria, and yellow fever vaccine is widely used. There are minimal data on the use of anti-JEV IgG, particularly the cited Diagnostic Automation assay, and the authors do not report any in-house validation. The optimal approach for detection of previous exposure or vaccination is a seroneutralisation assay involving contemporaneous testing to other flaviviruses. We understand that seroneutralisation requires considerable resources and technical expertise, but we would suggest that the authors consider verifying the anti-JEV IgG results by sending the samples to a reference laboratory for seroneutralisation testing. There is unequivocal evidence for the hyperendemicity of dengue virus and yellow fever vaccine use in Nigeria. In the absence of further testing, these results may be explained entirely by cross-reactivity with other flaviviruses, and do not represent evidence of JEV circulation in Nigeria. Kind regards, JOURNAL OF IMMUNOASSAY AND IMMUNOCHEMISTRY 2022, VOL. 43, NO. 4, 463–465 https://doi.org/10.1080/15321819.2022.2039184
{"title":"Flavivirus cross-reactivity would explain the apparent findings of Japanese encephalitis virus infection in Nigeria","authors":"T. Bharucha, Nicole Zitzmann, P. Newton, A. Dubot-Pérès, L. Turtle","doi":"10.1080/15321819.2022.2039184","DOIUrl":"https://doi.org/10.1080/15321819.2022.2039184","url":null,"abstract":"To the Editor, Journal of Immunoassay and Immunochemistry 21 January 2022 Re: Sero-evidence of silent Japanese Encephalitis Virus infection among inhabitants of Ilorin, North-central Nigeria: a call for active surveillance https://www.tandfonline.com/doi/abs/10.1080/15321819.2021.1993897 We read with interest the recent article published in the Journal of Immunoassay and Immunochemistry reporting Japanese encephalitis virus (JEV) serological testing in Nigeria. There is no doubt that there are ongoing changes in the geographical distribution of flaviviruses, and surveillance for the relevant disease syndromes would be helpful. To our knowledge, serological testing of this kind has not been performed before in Nigeria. JEV RNA was apparently detected in a patient with confirmed yellow fever in Angola; however, acute JEV infection was not corroborated by conventional IgM ELISA or seroneutralisation. As the authors discuss in the article, there is high cross-reactivity between flaviviruses, and very significant false positives with ELISA tests. We urge caution in the interpretation of the results. Anti-JEV IgG results cannot be interpreted alone, they require to be reported alongside testing for other endemic flaviviruses, most importantly anti-dengue virus and yellow fever virus IgG, and flavivirus vaccination history. Yellow fever is reported in Nigeria, and yellow fever vaccine is widely used. There are minimal data on the use of anti-JEV IgG, particularly the cited Diagnostic Automation assay, and the authors do not report any in-house validation. The optimal approach for detection of previous exposure or vaccination is a seroneutralisation assay involving contemporaneous testing to other flaviviruses. We understand that seroneutralisation requires considerable resources and technical expertise, but we would suggest that the authors consider verifying the anti-JEV IgG results by sending the samples to a reference laboratory for seroneutralisation testing. There is unequivocal evidence for the hyperendemicity of dengue virus and yellow fever vaccine use in Nigeria. In the absence of further testing, these results may be explained entirely by cross-reactivity with other flaviviruses, and do not represent evidence of JEV circulation in Nigeria. Kind regards, JOURNAL OF IMMUNOASSAY AND IMMUNOCHEMISTRY 2022, VOL. 43, NO. 4, 463–465 https://doi.org/10.1080/15321819.2022.2039184","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79535704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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