Pub Date : 2006-09-01DOI: 10.1080/15321810600735007
In the first chapter of the book, the author, Dr. Lundbad introduces and defines proteome and proteomics. For the benefit of the reader, we quote here the first part of the introduction with which we agree. “Proteomics is an increasingly complex area of study that is expected to yield results important for the development of therapeutics, diagnostics and for the emerging discipline of theranostics, which emphasizes patient-specific therapeutics. What, however, exactly is proteomics? The term proteome dates back to 1995 when Humphrey-Smith and colleagues defined the proteome as “the total protein content of a genome.” Genome is defined as “a complete single set of the genetic material of a cell or of an organism; the complete set of genes in a gamete.” It would follow that proteomics is the study of the proteome. A variety of other definitions have been proposed for proteomics. Morrison and coworkers define the proteome as “the entire complement of proteins expressed by a cell at a point in time.” In such cases, proteomics would be the study of the proteome; however, this definition would exclude extracellular collections of proteins such as those found in blood plasma, urine, and lymphatic fluid. These latter studies use some of the tools of proteomics, such as twodimensional electrophoresis and mass spectrometry, but are clearly different from studies where isotope-coded affinity tag (ICAT) technology is used to study differential protein expression and are used to identify biomarkers for diagnostics and therapeutics. Whatever the precise definition, proteomics involves the study of complex mixtures of proteins and their interactions. This somewhat broader definition might be useful in that it extends the application of proteomics to diagnostics. The technologies that underlie proteomics quite likely will improve sufficiently in analytical capability to be valuable in personalized medicine.” According to the author, “The overall intent of the current book is to address issues that are not discussed in detail by others and to avoid, where Journal of Immunoassay & Immunochemistry, 27: 289–290, 2006 Copyright # Taylor & Francis Group, LLC ISSN 1532-1819 print/1532-4230 online DOI: 10.1080/15321810600735007
在书的第一章中，作者伦巴德博士介绍了蛋白质组学和蛋白质组学。为了读者的利益，我们在这里引用我们同意的引言的第一部分。“蛋白质组学是一个日益复杂的研究领域，有望对治疗学、诊断学和新兴的治疗学学科(强调患者特异性治疗)的发展产生重要的结果。然而，蛋白质组学究竟是什么?蛋白质组这个术语可以追溯到1995年，当时汉弗莱-史密斯和他的同事将蛋白质组定义为“基因组的总蛋白质含量”。基因组被定义为“细胞或生物体的完整的单一遗传物质;配子中的全套基因。”因此，蛋白质组学就是对蛋白质组的研究。对于蛋白质组学，人们提出了许多其他的定义。莫里森和同事将蛋白质组定义为“细胞在某一时间点表达的全部蛋白质”。在这种情况下，蛋白质组学就是研究蛋白质组;然而，这一定义将排除细胞外的蛋白质集合，如在血浆、尿液和淋巴液中发现的蛋白质。后者的研究使用了蛋白质组学的一些工具，如二维电泳和质谱，但与使用同位素编码亲和标签(ICAT)技术研究差异蛋白表达并用于识别诊断和治疗的生物标志物的研究明显不同。无论精确的定义是什么，蛋白质组学涉及研究蛋白质的复杂混合物及其相互作用。这个更广泛的定义可能是有用的，因为它将蛋白质组学的应用扩展到诊断领域。蛋白质组学的基础技术很可能会充分提高分析能力，从而在个性化医疗中发挥重要作用。”根据作者的观点，“目前这本书的总体意图是解决其他人没有详细讨论和避免的问题，《免疫分析与免疫化学杂志》，27:289-290,2006版权# Taylor & Francis Group, LLC ISSN 1532-1819 print/1532-4230 online DOI: 10.1080/15321810600735007
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Pub Date : 2005-10-01DOI: 10.1080/15321810500220993
Peter L. McDermott
Industrial Proteomics, Applications for Biotechnology and Pharmaceuticals is as the editor states “is a focused treatment of industrial applications of proteomics. Proteomics in industry is generally focused on application in target discovery and pharmaceutical pipelines, thereby requiring proteomic processes that are robust, well characterized, under quality control (QC) and producing statistically significant results. It also requires the capacity to handle significant amounts of samples. For example, a simple clinical proteomic study might require an analysis by expression proteomics from a minimum of 36 to hundreds of complex samples.” This book contains 11 chapters, each of which addresses specific aspects of industrial application of proteomics. The first chapter covers the basics of mass spectrometry (MS)-based proteomics. Functional proteomics is covered in Chapters 2 and 3. Chapter 2 discusses the MS-based approaches of mapping protein interactions. Chapter 3 discusses the protein posttranslational modifications, particularly, protein phosphorylations. Structural proteomics is covered in Chapters 4 and 5. Chapter 4 covers the use of high-throughput crystallography and in silico methods for structure-based drug design. Chapter 5 describes the use of hydrogen/deuterium exchange mass spectrometry for high-throughput protein structure studies. The first applications of proteomics were in target discovery. Chapter 6, a discussion of the utilization of proteomics technologies for the identification as well as the validation of protein targets is given. The latest application of proteomics has been for the discovery of disease or drug-related biomarkers. Chapter 7 provides an overview of biomarker discovery and validation while Chapter 8 details plasma biomarker discovery using proteomics. Proteomics can also be approached from the small-molecule worked (i.e., drugs), particularly, to find proteins that interact with drugs. Chapter 9 presents chemical genomics/chemical proteomics and discusses the different approaches. Journal of Immunoassay & Immunochemistry, 26: 357–364, 2005 Copyright # Taylor & Francis, Inc. ISSN 1532-1819 print/1532-4230 online DOI: 10.1080/15321810500220993
工业蛋白质组学，生物技术和制药的应用，正如编辑所说，是蛋白质组学的工业应用的重点治疗。蛋白质组学在工业上的应用通常集中在靶点发现和制药管道中，因此要求蛋白质组学过程是稳健的，具有良好的特征，在质量控制(QC)下，并产生统计上显著的结果。它还需要处理大量样品的能力。例如，一项简单的临床蛋白质组学研究可能需要对至少36到数百个复杂样本进行表达蛋白质组学分析。”本书包含11章，每一章都涉及蛋白质组学工业应用的具体方面。第一章涵盖了基于质谱(MS)的蛋白质组学的基础知识。功能蛋白质组学将在第2章和第3章中介绍。第2章讨论了基于质谱的蛋白质相互作用制图方法。第3章讨论了蛋白质的翻译后修饰，特别是蛋白质磷酸化。结构蛋白质组学将在第4章和第5章中介绍。第4章涵盖了高通量晶体学和基于结构的药物设计的硅方法的使用。第5章描述了氢/氘交换质谱法在高通量蛋白质结构研究中的应用。蛋白质组学的第一个应用是靶标发现。第六章讨论了蛋白质组学技术在蛋白质靶点鉴定和验证中的应用。蛋白质组学的最新应用是发现疾病或药物相关的生物标志物。第7章概述了生物标志物的发现和验证，而第8章详细介绍了使用蛋白质组学发现血浆生物标志物。蛋白质组学也可以从小分子工作(即药物)着手，特别是寻找与药物相互作用的蛋白质。第9章介绍了化学基因组学/化学蛋白质组学，并讨论了不同的方法。免疫分析与免疫化学杂志，26:357-364,2005版权所有# Taylor & Francis, Inc。ISSN 1532-1819 print/1532-4230 online DOI: 10.1080/15321810500220993
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After the completion of the sequencing phase of the Human Genome Project, the focus of contemporary science shifts to reveal gene functions, i.e., the examination of proteins that are encoded within. The goal of chemical genomics is to dissect the function of organisms and cells by having a small-molecule partner for every gene product. Chemical genomics holds the promise for the determination of the function and biological role of any genes through small-molecule interactions with the protein or proteins that is expressed by that particular gene. In addition, various chemical genomics methods can address such biological questions that are not amenable to genetic manipulation or to structural genomic approaches. In the last couple of years, significant advances have been made in the fields of genomics-driven drug discovery, chemoinformatics, and high-throughput screening, proven by the increasing number of papers that have appeared in the literature already utilizing chemical genomics tools. This book is dedicated solely to chemical genomics, discussing a full spectrum of chemical genomics topics, as well as related technologies ranging from in silico approaches to experimental techniques. The first part describes the definition and basics of chemical genomics. The second part focuses on specific approaches, discussing the generation and utility of small-molecule probes in the study of specific gene products. The last three chapters are practical case studies related to the area of drug discovery. This book provides an overview of this emerging field to practitioners in drug discovery, medicinal chemistry, and molecular biology as well as scientists working in the laboratories and for students at the graduate and undergraduate levels. It is well written and presented.
{"title":"The Book Corner","authors":"F. Darvas, A. Guttman, G. Dormán","doi":"10.1081/IAS-200062503","DOIUrl":"https://doi.org/10.1081/IAS-200062503","url":null,"abstract":"After the completion of the sequencing phase of the Human Genome Project, the focus of contemporary science shifts to reveal gene functions, i.e., the examination of proteins that are encoded within. The goal of chemical genomics is to dissect the function of organisms and cells by having a small-molecule partner for every gene product. Chemical genomics holds the promise for the determination of the function and biological role of any genes through small-molecule interactions with the protein or proteins that is expressed by that particular gene. In addition, various chemical genomics methods can address such biological questions that are not amenable to genetic manipulation or to structural genomic approaches. In the last couple of years, significant advances have been made in the fields of genomics-driven drug discovery, chemoinformatics, and high-throughput screening, proven by the increasing number of papers that have appeared in the literature already utilizing chemical genomics tools. This book is dedicated solely to chemical genomics, discussing a full spectrum of chemical genomics topics, as well as related technologies ranging from in silico approaches to experimental techniques. The first part describes the definition and basics of chemical genomics. The second part focuses on specific approaches, discussing the generation and utility of small-molecule probes in the study of specific gene products. The last three chapters are practical case studies related to the area of drug discovery. This book provides an overview of this emerging field to practitioners in drug discovery, medicinal chemistry, and molecular biology as well as scientists working in the laboratories and for students at the graduate and undergraduate levels. It is well written and presented.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87981996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated monoand oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of monoand oligosaccharides, glycoproteins, and glycoconjugates. Glycoconjugates, such as glycoproteins and glycolipids, play important roles in cell–cell interaction events, including development, differentiation, morphogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to protein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive coor post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique substrates. The molecular biology of these transferases and other carbohydrate-modifying enzymes is providing important insights
{"title":"The Book Corner","authors":"","doi":"10.1081/IAS-120030530","DOIUrl":"https://doi.org/10.1081/IAS-120030530","url":null,"abstract":"Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated monoand oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of monoand oligosaccharides, glycoproteins, and glycoconjugates. Glycoconjugates, such as glycoproteins and glycolipids, play important roles in cell–cell interaction events, including development, differentiation, morphogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to protein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive coor post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique substrates. The molecular biology of these transferases and other carbohydrate-modifying enzymes is providing important insights","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80592751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This is Volume 128 in the “Drugs and the Pharmaceutical Sciences” Series. This volume gives a comprehensive review of another aspect of capillary electrophoresis (CE), namely affinity CE. Since the pioneering accomplishments of Hjertén and, particularly, of Jorgenson and Lukacs, CE has undergone a dynamic development, producing a variety of applications. In chemical and pharmaceutical analysis, CE was employed mainly to separate and quantify drugs; this subject has recently been reviewed. The implementation of CE in quality control or drug profiling in biological systems has been illustrated in numerous studies. Capillary electrophoresis separations can be performed in different modes, using the same technical equipment. Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC), introduced by Terabe et al., are most frequently employed. The first part of this book presents theoretical basics necessary to understand the principles and techniques of CE, as well as ACE. This knowledge opens access to potential applications in pharmaceutics, e.g., the investigation of interaction partners improving the solubility of lipophilic and barely watersoluble drugs and the determination of the effects of amphiphilic ion-pairing or complexation reagents (e.g., pharmaceutical excipients) on the permeation as well as absorption behavior of hydrophilic drugs. ACE enables the calculation of equilibrium constants, which are a measure of the strength of interaction. Although MEKC and ACE are based on the same principle, the recent
{"title":"The Book Corner","authors":"R. Neubert","doi":"10.1081/IAS-200028102","DOIUrl":"https://doi.org/10.1081/IAS-200028102","url":null,"abstract":"This is Volume 128 in the “Drugs and the Pharmaceutical Sciences” Series. This volume gives a comprehensive review of another aspect of capillary electrophoresis (CE), namely affinity CE. Since the pioneering accomplishments of Hjertén and, particularly, of Jorgenson and Lukacs, CE has undergone a dynamic development, producing a variety of applications. In chemical and pharmaceutical analysis, CE was employed mainly to separate and quantify drugs; this subject has recently been reviewed. The implementation of CE in quality control or drug profiling in biological systems has been illustrated in numerous studies. Capillary electrophoresis separations can be performed in different modes, using the same technical equipment. Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC), introduced by Terabe et al., are most frequently employed. The first part of this book presents theoretical basics necessary to understand the principles and techniques of CE, as well as ACE. This knowledge opens access to potential applications in pharmaceutics, e.g., the investigation of interaction partners improving the solubility of lipophilic and barely watersoluble drugs and the determination of the effects of amphiphilic ion-pairing or complexation reagents (e.g., pharmaceutical excipients) on the permeation as well as absorption behavior of hydrophilic drugs. ACE enables the calculation of equilibrium constants, which are a measure of the strength of interaction. Although MEKC and ACE are based on the same principle, the recent","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80074794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meeting Announcement","authors":"","doi":"10.1081/ias-120030529","DOIUrl":"https://doi.org/10.1081/ias-120030529","url":null,"abstract":"","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86059939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meetings and Symposia","authors":"J. Cunningham","doi":"10.1081/IAS-120025860","DOIUrl":"https://doi.org/10.1081/IAS-120025860","url":null,"abstract":"","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87889056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction to Environmental Analysis is volume number three in the Analytical Techniques in the Sciences series, D. J. Ando, series editor. The other two published volumes in this series are Analytical Instrumentation: Performance Characteristics, and Quality and Fundamentals of Electroanalytical Chemistry. Interest in the environment continues to expand and develop. It is now very much a part of our everyday lives. As a consequence, the need for chemical analysis of the environment continues to grow. This book is a revision and expansion of the ACOL text ‘‘Environmental analysis’’ which was first published in 1994. It is an introduction into how chemical analytical techniques are applied to the environment. Global awareness of the importance of monitoring and protecting our environment has grown considerably over the last ten years. Environmental concerns are now an integral part of today’s legislation, product design and development, waste minimization and disposal. As well as background monitoring of the environment, scientists are involved in monitoring liquid and gaseous discharges and surveying contaminated land and landfill sites—very topical issues due to concern over waste duping and potential problems with reuse of old industrial sites. Introduction to Environmental Analysis introduces the reader to the methodology required to monitor our environment and to safeguard it. The present book is made up of eight chapters which are well presented
{"title":"The Book Corner","authors":"M. Dekker","doi":"10.1081/IAS-120020087","DOIUrl":"https://doi.org/10.1081/IAS-120020087","url":null,"abstract":"Introduction to Environmental Analysis is volume number three in the Analytical Techniques in the Sciences series, D. J. Ando, series editor. The other two published volumes in this series are Analytical Instrumentation: Performance Characteristics, and Quality and Fundamentals of Electroanalytical Chemistry. Interest in the environment continues to expand and develop. It is now very much a part of our everyday lives. As a consequence, the need for chemical analysis of the environment continues to grow. This book is a revision and expansion of the ACOL text ‘‘Environmental analysis’’ which was first published in 1994. It is an introduction into how chemical analytical techniques are applied to the environment. Global awareness of the importance of monitoring and protecting our environment has grown considerably over the last ten years. Environmental concerns are now an integral part of today’s legislation, product design and development, waste minimization and disposal. As well as background monitoring of the environment, scientists are involved in monitoring liquid and gaseous discharges and surveying contaminated land and landfill sites—very topical issues due to concern over waste duping and potential problems with reuse of old industrial sites. Introduction to Environmental Analysis introduces the reader to the methodology required to monitor our environment and to safeguard it. The present book is made up of eight chapters which are well presented","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88161640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. C. Abrahantes, Biunayki Reyes, J. Reyes, Raudel Sosa, E. González, Maribel Vega, R. Valdés, E. Martínez
ABSTRACT Mabs against HBsAg have been used for structural analyses, development of diagnostic tests, and for antigen immunopurification. Resultant products obtained from current methods of genetic recombination demand reference materials to test their potency, identity, purity, and the biological and immunological specific activity corresponding to their manufacturing processes. In this paper, we present a method for the qualitative and quantitative characterisation of CB.Hep-1 and CB.Hep-4 RM and their stability, in real time, with the required quality to be used as primary reference materials. Among the criteria applied in this study, we considered Mab-specific concentration through ELISA, purity, and total proteins by various methods, quantification of antigen recognition capacity of Mabs through the Ag-Mab absolute- recognition method. Sterility, homogeneity, stability, subclass, and isoelectric focusing were used in the characterisation of the reference materials.
{"title":"PROCUREMENT OF REFERENCE MATERIALS AGAINST RECOMBINANT HBsAG SURFACE ANTIGEN","authors":"M. C. Abrahantes, Biunayki Reyes, J. Reyes, Raudel Sosa, E. González, Maribel Vega, R. Valdés, E. Martínez","doi":"10.1081/IAS-120003663","DOIUrl":"https://doi.org/10.1081/IAS-120003663","url":null,"abstract":"ABSTRACT Mabs against HBsAg have been used for structural analyses, development of diagnostic tests, and for antigen immunopurification. Resultant products obtained from current methods of genetic recombination demand reference materials to test their potency, identity, purity, and the biological and immunological specific activity corresponding to their manufacturing processes. In this paper, we present a method for the qualitative and quantitative characterisation of CB.Hep-1 and CB.Hep-4 RM and their stability, in real time, with the required quality to be used as primary reference materials. Among the criteria applied in this study, we considered Mab-specific concentration through ELISA, purity, and total proteins by various methods, quantification of antigen recognition capacity of Mabs through the Ag-Mab absolute- recognition method. Sterility, homogeneity, stability, subclass, and isoelectric focusing were used in the characterisation of the reference materials.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89233168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Bard, D. Riethmuller, S. Biichle, D. Meillet, J. Prétet, C. Mougin, E. Seillès
ABSTRACT Several studies were carried out to characterize the humoral immune response on mucosal genital surfaces. However, the results obtained so far were particularly conflicting due to the absence of validation methods. The aim of this study was to develop and validate a quantitative ELISA method, which is sensitive and reproducible, to measure immunoglobulin and secretory immunoglobulin concentrations in various biological fluids. This quantitative, sensitive (detection limit = 1 µg/L) and reproducible (coefficient of variation <15%) method could be of interest to study the effects of viral infections on mucosal non-specific immune response in genital tract. To explore the humoral response, serum, saliva, vaginal secretions, and cervicovaginal secretions from 18 women, 20–45 years old, were evaluated for total-IgA, secretory IgA, IgM, and IgG. Albumin level was also evaluated by immuno-nephelometry. The secretion rates of immunoglobulins were measured by calculating their relative coefficients of excretion by reference to albumin. Despite large individual variations, median immunoglobulin levels were higher in the endocervical secretions than in the cervicovaginal secretions. When we compared the rates of immunoglobulins in genital fluids, IgG prevalence was higher (80%) in cervicovaginal and endocervical secretions than IgA prevalence (12%). In contrast, digestive mucosal secretions, such as saliva, contained mostly IgA (80%). In cervicovaginal and endocervical secretions, IgG and IgM originated mainly from serum, whereas a local synthesis provided total-IgA and secretory IgA. These results allowed us to raise a possible hypothesis for the origin of immunoglobulins in the genital tract. They illustrated the peculiar feature of the female reproductive tract and the difficulty for this tissue to contribute in the mucosal associated lymphoid tissue. The low secretory-IgA and total-IgA levels could explain the particular sensitivity of the vagina and the cervix to infections.
{"title":"VALIDATION OF A HIGH SENSITIVE IMMUNOENZYMATIC ASSAY TO ESTABLISH THE ORIGIN OF IMMUNOGLOBULINS IN FEMALE GENITAL SECRETIONS","authors":"E. Bard, D. Riethmuller, S. Biichle, D. Meillet, J. Prétet, C. Mougin, E. Seillès","doi":"10.1081/IAS-120003658","DOIUrl":"https://doi.org/10.1081/IAS-120003658","url":null,"abstract":"ABSTRACT Several studies were carried out to characterize the humoral immune response on mucosal genital surfaces. However, the results obtained so far were particularly conflicting due to the absence of validation methods. The aim of this study was to develop and validate a quantitative ELISA method, which is sensitive and reproducible, to measure immunoglobulin and secretory immunoglobulin concentrations in various biological fluids. This quantitative, sensitive (detection limit = 1 µg/L) and reproducible (coefficient of variation <15%) method could be of interest to study the effects of viral infections on mucosal non-specific immune response in genital tract. To explore the humoral response, serum, saliva, vaginal secretions, and cervicovaginal secretions from 18 women, 20–45 years old, were evaluated for total-IgA, secretory IgA, IgM, and IgG. Albumin level was also evaluated by immuno-nephelometry. The secretion rates of immunoglobulins were measured by calculating their relative coefficients of excretion by reference to albumin. Despite large individual variations, median immunoglobulin levels were higher in the endocervical secretions than in the cervicovaginal secretions. When we compared the rates of immunoglobulins in genital fluids, IgG prevalence was higher (80%) in cervicovaginal and endocervical secretions than IgA prevalence (12%). In contrast, digestive mucosal secretions, such as saliva, contained mostly IgA (80%). In cervicovaginal and endocervical secretions, IgG and IgM originated mainly from serum, whereas a local synthesis provided total-IgA and secretory IgA. These results allowed us to raise a possible hypothesis for the origin of immunoglobulins in the genital tract. They illustrated the peculiar feature of the female reproductive tract and the difficulty for this tissue to contribute in the mucosal associated lymphoid tissue. The low secretory-IgA and total-IgA levels could explain the particular sensitivity of the vagina and the cervix to infections.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90069466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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