After the completion of the sequencing phase of the Human Genome Project, the focus of contemporary science shifts to reveal gene functions, i.e., the examination of proteins that are encoded within. The goal of chemical genomics is to dissect the function of organisms and cells by having a small-molecule partner for every gene product. Chemical genomics holds the promise for the determination of the function and biological role of any genes through small-molecule interactions with the protein or proteins that is expressed by that particular gene. In addition, various chemical genomics methods can address such biological questions that are not amenable to genetic manipulation or to structural genomic approaches. In the last couple of years, significant advances have been made in the fields of genomics-driven drug discovery, chemoinformatics, and high-throughput screening, proven by the increasing number of papers that have appeared in the literature already utilizing chemical genomics tools. This book is dedicated solely to chemical genomics, discussing a full spectrum of chemical genomics topics, as well as related technologies ranging from in silico approaches to experimental techniques. The first part describes the definition and basics of chemical genomics. The second part focuses on specific approaches, discussing the generation and utility of small-molecule probes in the study of specific gene products. The last three chapters are practical case studies related to the area of drug discovery. This book provides an overview of this emerging field to practitioners in drug discovery, medicinal chemistry, and molecular biology as well as scientists working in the laboratories and for students at the graduate and undergraduate levels. It is well written and presented.
{"title":"The Book Corner","authors":"F. Darvas, A. Guttman, G. Dormán","doi":"10.1081/IAS-200062503","DOIUrl":"https://doi.org/10.1081/IAS-200062503","url":null,"abstract":"After the completion of the sequencing phase of the Human Genome Project, the focus of contemporary science shifts to reveal gene functions, i.e., the examination of proteins that are encoded within. The goal of chemical genomics is to dissect the function of organisms and cells by having a small-molecule partner for every gene product. Chemical genomics holds the promise for the determination of the function and biological role of any genes through small-molecule interactions with the protein or proteins that is expressed by that particular gene. In addition, various chemical genomics methods can address such biological questions that are not amenable to genetic manipulation or to structural genomic approaches. In the last couple of years, significant advances have been made in the fields of genomics-driven drug discovery, chemoinformatics, and high-throughput screening, proven by the increasing number of papers that have appeared in the literature already utilizing chemical genomics tools. This book is dedicated solely to chemical genomics, discussing a full spectrum of chemical genomics topics, as well as related technologies ranging from in silico approaches to experimental techniques. The first part describes the definition and basics of chemical genomics. The second part focuses on specific approaches, discussing the generation and utility of small-molecule probes in the study of specific gene products. The last three chapters are practical case studies related to the area of drug discovery. This book provides an overview of this emerging field to practitioners in drug discovery, medicinal chemistry, and molecular biology as well as scientists working in the laboratories and for students at the graduate and undergraduate levels. It is well written and presented.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"99 1","pages":"245 - 249"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87981996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated monoand oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of monoand oligosaccharides, glycoproteins, and glycoconjugates. Glycoconjugates, such as glycoproteins and glycolipids, play important roles in cell–cell interaction events, including development, differentiation, morphogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to protein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive coor post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique substrates. The molecular biology of these transferases and other carbohydrate-modifying enzymes is providing important insights
{"title":"The Book Corner","authors":"","doi":"10.1081/IAS-120030530","DOIUrl":"https://doi.org/10.1081/IAS-120030530","url":null,"abstract":"Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated monoand oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of monoand oligosaccharides, glycoproteins, and glycoconjugates. Glycoconjugates, such as glycoproteins and glycolipids, play important roles in cell–cell interaction events, including development, differentiation, morphogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to protein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive coor post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique substrates. The molecular biology of these transferases and other carbohydrate-modifying enzymes is providing important insights","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"100 1","pages":"195 - 203"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80592751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This is Volume 128 in the “Drugs and the Pharmaceutical Sciences” Series. This volume gives a comprehensive review of another aspect of capillary electrophoresis (CE), namely affinity CE. Since the pioneering accomplishments of Hjertén and, particularly, of Jorgenson and Lukacs, CE has undergone a dynamic development, producing a variety of applications. In chemical and pharmaceutical analysis, CE was employed mainly to separate and quantify drugs; this subject has recently been reviewed. The implementation of CE in quality control or drug profiling in biological systems has been illustrated in numerous studies. Capillary electrophoresis separations can be performed in different modes, using the same technical equipment. Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC), introduced by Terabe et al., are most frequently employed. The first part of this book presents theoretical basics necessary to understand the principles and techniques of CE, as well as ACE. This knowledge opens access to potential applications in pharmaceutics, e.g., the investigation of interaction partners improving the solubility of lipophilic and barely watersoluble drugs and the determination of the effects of amphiphilic ion-pairing or complexation reagents (e.g., pharmaceutical excipients) on the permeation as well as absorption behavior of hydrophilic drugs. ACE enables the calculation of equilibrium constants, which are a measure of the strength of interaction. Although MEKC and ACE are based on the same principle, the recent
{"title":"The Book Corner","authors":"R. Neubert","doi":"10.1081/IAS-200028102","DOIUrl":"https://doi.org/10.1081/IAS-200028102","url":null,"abstract":"This is Volume 128 in the “Drugs and the Pharmaceutical Sciences” Series. This volume gives a comprehensive review of another aspect of capillary electrophoresis (CE), namely affinity CE. Since the pioneering accomplishments of Hjertén and, particularly, of Jorgenson and Lukacs, CE has undergone a dynamic development, producing a variety of applications. In chemical and pharmaceutical analysis, CE was employed mainly to separate and quantify drugs; this subject has recently been reviewed. The implementation of CE in quality control or drug profiling in biological systems has been illustrated in numerous studies. Capillary electrophoresis separations can be performed in different modes, using the same technical equipment. Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC), introduced by Terabe et al., are most frequently employed. The first part of this book presents theoretical basics necessary to understand the principles and techniques of CE, as well as ACE. This knowledge opens access to potential applications in pharmaceutics, e.g., the investigation of interaction partners improving the solubility of lipophilic and barely watersoluble drugs and the determination of the effects of amphiphilic ion-pairing or complexation reagents (e.g., pharmaceutical excipients) on the permeation as well as absorption behavior of hydrophilic drugs. ACE enables the calculation of equilibrium constants, which are a measure of the strength of interaction. Although MEKC and ACE are based on the same principle, the recent","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"22 1","pages":"305 - 311"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80074794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meeting Announcement","authors":"","doi":"10.1081/ias-120030529","DOIUrl":"https://doi.org/10.1081/ias-120030529","url":null,"abstract":"","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"6 1","pages":"193 - 194"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86059939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meetings and Symposia","authors":"J. Cunningham","doi":"10.1081/IAS-120025860","DOIUrl":"https://doi.org/10.1081/IAS-120025860","url":null,"abstract":"","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"10 1","pages":"421 - 425"},"PeriodicalIF":0.0,"publicationDate":"2003-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87889056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction to Environmental Analysis is volume number three in the Analytical Techniques in the Sciences series, D. J. Ando, series editor. The other two published volumes in this series are Analytical Instrumentation: Performance Characteristics, and Quality and Fundamentals of Electroanalytical Chemistry. Interest in the environment continues to expand and develop. It is now very much a part of our everyday lives. As a consequence, the need for chemical analysis of the environment continues to grow. This book is a revision and expansion of the ACOL text ‘‘Environmental analysis’’ which was first published in 1994. It is an introduction into how chemical analytical techniques are applied to the environment. Global awareness of the importance of monitoring and protecting our environment has grown considerably over the last ten years. Environmental concerns are now an integral part of today’s legislation, product design and development, waste minimization and disposal. As well as background monitoring of the environment, scientists are involved in monitoring liquid and gaseous discharges and surveying contaminated land and landfill sites—very topical issues due to concern over waste duping and potential problems with reuse of old industrial sites. Introduction to Environmental Analysis introduces the reader to the methodology required to monitor our environment and to safeguard it. The present book is made up of eight chapters which are well presented
{"title":"The Book Corner","authors":"M. Dekker","doi":"10.1081/IAS-120020087","DOIUrl":"https://doi.org/10.1081/IAS-120020087","url":null,"abstract":"Introduction to Environmental Analysis is volume number three in the Analytical Techniques in the Sciences series, D. J. Ando, series editor. The other two published volumes in this series are Analytical Instrumentation: Performance Characteristics, and Quality and Fundamentals of Electroanalytical Chemistry. Interest in the environment continues to expand and develop. It is now very much a part of our everyday lives. As a consequence, the need for chemical analysis of the environment continues to grow. This book is a revision and expansion of the ACOL text ‘‘Environmental analysis’’ which was first published in 1994. It is an introduction into how chemical analytical techniques are applied to the environment. Global awareness of the importance of monitoring and protecting our environment has grown considerably over the last ten years. Environmental concerns are now an integral part of today’s legislation, product design and development, waste minimization and disposal. As well as background monitoring of the environment, scientists are involved in monitoring liquid and gaseous discharges and surveying contaminated land and landfill sites—very topical issues due to concern over waste duping and potential problems with reuse of old industrial sites. Introduction to Environmental Analysis introduces the reader to the methodology required to monitor our environment and to safeguard it. The present book is made up of eight chapters which are well presented","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"8 1","pages":"233 - 242"},"PeriodicalIF":0.0,"publicationDate":"2003-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88161640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. C. Abrahantes, Biunayki Reyes, J. Reyes, Raudel Sosa, E. González, Maribel Vega, R. Valdés, E. Martínez
ABSTRACT Mabs against HBsAg have been used for structural analyses, development of diagnostic tests, and for antigen immunopurification. Resultant products obtained from current methods of genetic recombination demand reference materials to test their potency, identity, purity, and the biological and immunological specific activity corresponding to their manufacturing processes. In this paper, we present a method for the qualitative and quantitative characterisation of CB.Hep-1 and CB.Hep-4 RM and their stability, in real time, with the required quality to be used as primary reference materials. Among the criteria applied in this study, we considered Mab-specific concentration through ELISA, purity, and total proteins by various methods, quantification of antigen recognition capacity of Mabs through the Ag-Mab absolute- recognition method. Sterility, homogeneity, stability, subclass, and isoelectric focusing were used in the characterisation of the reference materials.
{"title":"PROCUREMENT OF REFERENCE MATERIALS AGAINST RECOMBINANT HBsAG SURFACE ANTIGEN","authors":"M. C. Abrahantes, Biunayki Reyes, J. Reyes, Raudel Sosa, E. González, Maribel Vega, R. Valdés, E. Martínez","doi":"10.1081/IAS-120003663","DOIUrl":"https://doi.org/10.1081/IAS-120003663","url":null,"abstract":"ABSTRACT Mabs against HBsAg have been used for structural analyses, development of diagnostic tests, and for antigen immunopurification. Resultant products obtained from current methods of genetic recombination demand reference materials to test their potency, identity, purity, and the biological and immunological specific activity corresponding to their manufacturing processes. In this paper, we present a method for the qualitative and quantitative characterisation of CB.Hep-1 and CB.Hep-4 RM and their stability, in real time, with the required quality to be used as primary reference materials. Among the criteria applied in this study, we considered Mab-specific concentration through ELISA, purity, and total proteins by various methods, quantification of antigen recognition capacity of Mabs through the Ag-Mab absolute- recognition method. Sterility, homogeneity, stability, subclass, and isoelectric focusing were used in the characterisation of the reference materials.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"6 1","pages":"229 - 244"},"PeriodicalIF":0.0,"publicationDate":"2002-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89233168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Bard, D. Riethmuller, S. Biichle, D. Meillet, J. Prétet, C. Mougin, E. Seillès
ABSTRACT Several studies were carried out to characterize the humoral immune response on mucosal genital surfaces. However, the results obtained so far were particularly conflicting due to the absence of validation methods. The aim of this study was to develop and validate a quantitative ELISA method, which is sensitive and reproducible, to measure immunoglobulin and secretory immunoglobulin concentrations in various biological fluids. This quantitative, sensitive (detection limit = 1 µg/L) and reproducible (coefficient of variation <15%) method could be of interest to study the effects of viral infections on mucosal non-specific immune response in genital tract. To explore the humoral response, serum, saliva, vaginal secretions, and cervicovaginal secretions from 18 women, 20–45 years old, were evaluated for total-IgA, secretory IgA, IgM, and IgG. Albumin level was also evaluated by immuno-nephelometry. The secretion rates of immunoglobulins were measured by calculating their relative coefficients of excretion by reference to albumin. Despite large individual variations, median immunoglobulin levels were higher in the endocervical secretions than in the cervicovaginal secretions. When we compared the rates of immunoglobulins in genital fluids, IgG prevalence was higher (80%) in cervicovaginal and endocervical secretions than IgA prevalence (12%). In contrast, digestive mucosal secretions, such as saliva, contained mostly IgA (80%). In cervicovaginal and endocervical secretions, IgG and IgM originated mainly from serum, whereas a local synthesis provided total-IgA and secretory IgA. These results allowed us to raise a possible hypothesis for the origin of immunoglobulins in the genital tract. They illustrated the peculiar feature of the female reproductive tract and the difficulty for this tissue to contribute in the mucosal associated lymphoid tissue. The low secretory-IgA and total-IgA levels could explain the particular sensitivity of the vagina and the cervix to infections.
{"title":"VALIDATION OF A HIGH SENSITIVE IMMUNOENZYMATIC ASSAY TO ESTABLISH THE ORIGIN OF IMMUNOGLOBULINS IN FEMALE GENITAL SECRETIONS","authors":"E. Bard, D. Riethmuller, S. Biichle, D. Meillet, J. Prétet, C. Mougin, E. Seillès","doi":"10.1081/IAS-120003658","DOIUrl":"https://doi.org/10.1081/IAS-120003658","url":null,"abstract":"ABSTRACT Several studies were carried out to characterize the humoral immune response on mucosal genital surfaces. However, the results obtained so far were particularly conflicting due to the absence of validation methods. The aim of this study was to develop and validate a quantitative ELISA method, which is sensitive and reproducible, to measure immunoglobulin and secretory immunoglobulin concentrations in various biological fluids. This quantitative, sensitive (detection limit = 1 µg/L) and reproducible (coefficient of variation <15%) method could be of interest to study the effects of viral infections on mucosal non-specific immune response in genital tract. To explore the humoral response, serum, saliva, vaginal secretions, and cervicovaginal secretions from 18 women, 20–45 years old, were evaluated for total-IgA, secretory IgA, IgM, and IgG. Albumin level was also evaluated by immuno-nephelometry. The secretion rates of immunoglobulins were measured by calculating their relative coefficients of excretion by reference to albumin. Despite large individual variations, median immunoglobulin levels were higher in the endocervical secretions than in the cervicovaginal secretions. When we compared the rates of immunoglobulins in genital fluids, IgG prevalence was higher (80%) in cervicovaginal and endocervical secretions than IgA prevalence (12%). In contrast, digestive mucosal secretions, such as saliva, contained mostly IgA (80%). In cervicovaginal and endocervical secretions, IgG and IgM originated mainly from serum, whereas a local synthesis provided total-IgA and secretory IgA. These results allowed us to raise a possible hypothesis for the origin of immunoglobulins in the genital tract. They illustrated the peculiar feature of the female reproductive tract and the difficulty for this tissue to contribute in the mucosal associated lymphoid tissue. The low secretory-IgA and total-IgA levels could explain the particular sensitivity of the vagina and the cervix to infections.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"1 1","pages":"145 - 162"},"PeriodicalIF":0.0,"publicationDate":"2002-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90069466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Aboul‐Enein, Stefan Raluca-Ioana, S. Lițescu, G. Radu
ABSTRACT An amperometric biosensor based on L-aminoacid oxidase is proposed for enantioselective assay of (+)-3,3′,5-triiodo-L-thyronine (L-T3) and (+)-3,3′,5,5′-tetraiodo-L-thyronine (L-T4), due to the fact that only the L enantiomer has the hormonal activity. The construction of the amperometric biosensor is simple and reproducible. The analytical information obtained from enantioselective analysis are reliable. The RSD <1% assured by using the amperometric biosensors for L enantiomers assay as raw materials, and from tablets, demonstrated their suitability for the analysis of T3 and T4 at ppb concentration levels.
{"title":"BIOSENSOR FOR THE ENANTIOSELECTIVE ANALYSIS OF THE THYROID HORMONES (+)-3,3′,5-TRIIODO-L-THYRONINE (T3) AND (+)-3,3′,5,5′-TETRAIODO-L-THYRONINE (T4)","authors":"H. Aboul‐Enein, Stefan Raluca-Ioana, S. Lițescu, G. Radu","doi":"10.1081/IAS-120003660","DOIUrl":"https://doi.org/10.1081/IAS-120003660","url":null,"abstract":"ABSTRACT An amperometric biosensor based on L-aminoacid oxidase is proposed for enantioselective assay of (+)-3,3′,5-triiodo-L-thyronine (L-T3) and (+)-3,3′,5,5′-tetraiodo-L-thyronine (L-T4), due to the fact that only the L enantiomer has the hormonal activity. The construction of the amperometric biosensor is simple and reproducible. The analytical information obtained from enantioselective analysis are reliable. The RSD <1% assured by using the amperometric biosensors for L enantiomers assay as raw materials, and from tablets, demonstrated their suitability for the analysis of T3 and T4 at ppb concentration levels.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"600 1","pages":"181 - 190"},"PeriodicalIF":0.0,"publicationDate":"2002-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77285200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT At our Institute, a panel of reproductive hormones, viz., estrone glucuronide (E1G), pregnanediol glucuronide (PdG), luteinising hormone (LH), and follicle stimulating hormone (FSH) are estimated by ELISA for the assessment of fertility from a single urine sample collected from a subject. In order to make the estimates less cumbersome, the selection and mode of presentation of immunoreagents of the assay were modified in such a way that, either on reconstitution or single dilution, would result in ready-to-use reagents in the assay. Retrospective analysis on the performance of these ELISAs with uniform protocols (n = 86) was compared with assays having individual assay protocols (n = 116). The performance of the assay, based on the standard curve characteristics and quality control pools, was better and the rate of acceptance of these assays improved from 87.9 to 97.6%. The simplification of assay protocols, thus, had better impact on the quality and reproducibility of immunoassay of the four analytes.
{"title":"IMPROVED PERFORMANCE OF ELISAs FOR FERTILITY ASSESSMENT USING COMMON REAGENTS AND ASSAY PROTOCOL AS EVIDENCE FROM QUALITY CONTROL STUDIES","authors":"M. Desai, U. Donde","doi":"10.1081/IAS-120003659","DOIUrl":"https://doi.org/10.1081/IAS-120003659","url":null,"abstract":"ABSTRACT At our Institute, a panel of reproductive hormones, viz., estrone glucuronide (E1G), pregnanediol glucuronide (PdG), luteinising hormone (LH), and follicle stimulating hormone (FSH) are estimated by ELISA for the assessment of fertility from a single urine sample collected from a subject. In order to make the estimates less cumbersome, the selection and mode of presentation of immunoreagents of the assay were modified in such a way that, either on reconstitution or single dilution, would result in ready-to-use reagents in the assay. Retrospective analysis on the performance of these ELISAs with uniform protocols (n = 86) was compared with assays having individual assay protocols (n = 116). The performance of the assay, based on the standard curve characteristics and quality control pools, was better and the rate of acceptance of these assays improved from 87.9 to 97.6%. The simplification of assay protocols, thus, had better impact on the quality and reproducibility of immunoassay of the four analytes.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"70 1","pages":"163 - 180"},"PeriodicalIF":0.0,"publicationDate":"2002-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86272855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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