Runa Sugimura, Toshiki Takamizawa, Ryota Aoki, Rei Muto, Eizo Hirokane, Hiroyasu Kurokawa, Nobuya Kitahara, Masashi Miyazaki
Objective: The color matching of single-shade resin composites after in-office whitening was investigated.
Materials and methods: Four single-shade resin composites were used. A total of 35% hydrogen peroxide was used as the whitening agent. The resin composite was placed in a cavity of an artificially discolored bovine tooth. The color differences between the restoration and surrounding enamel before and after whitening were determined based on ΔE*ab, ΔE00, and ΔWID. The color stability, surface roughness (Sa), and surface gloss (GU) of the resin composite alone were also evaluated. Statistical analyses were performed using repeated-measures analysis of variance with the Tukey-Kramer test.
Results: Based on the 50:50% of perceptibility and acceptability thresholds of ΔE*ab and ΔE00, none of the resin composite restorations were clinically acceptable before or after whitening. Regarding ΔWID, although all resin composites showed "acceptable match" in the baseline, they showed "mismatch" after the third session of whitening. Most of the resin composites alone were stable in color against whitening.
Conclusions: Although the single-shade resin composites failed to achieve the expected color matching on discolored teeth either before or after the whitening, the impact of the whitening on the color of the resin composite alone may be negligible.
{"title":"Influence of in-office whitening on the color matching and surface characteristics of single-shade resin composites.","authors":"Runa Sugimura, Toshiki Takamizawa, Ryota Aoki, Rei Muto, Eizo Hirokane, Hiroyasu Kurokawa, Nobuya Kitahara, Masashi Miyazaki","doi":"10.1111/jerd.13279","DOIUrl":"https://doi.org/10.1111/jerd.13279","url":null,"abstract":"<p><strong>Objective: </strong>The color matching of single-shade resin composites after in-office whitening was investigated.</p><p><strong>Materials and methods: </strong>Four single-shade resin composites were used. A total of 35% hydrogen peroxide was used as the whitening agent. The resin composite was placed in a cavity of an artificially discolored bovine tooth. The color differences between the restoration and surrounding enamel before and after whitening were determined based on ΔE*<sub>ab</sub>, ΔE<sub>00</sub>, and ΔWI<sub>D</sub>. The color stability, surface roughness (Sa), and surface gloss (GU) of the resin composite alone were also evaluated. Statistical analyses were performed using repeated-measures analysis of variance with the Tukey-Kramer test.</p><p><strong>Results: </strong>Based on the 50:50% of perceptibility and acceptability thresholds of ΔE*<sub>ab</sub> and ΔE<sub>00</sub>, none of the resin composite restorations were clinically acceptable before or after whitening. Regarding ΔWI<sub>D</sub>, although all resin composites showed \"acceptable match\" in the baseline, they showed \"mismatch\" after the third session of whitening. Most of the resin composites alone were stable in color against whitening.</p><p><strong>Conclusions: </strong>Although the single-shade resin composites failed to achieve the expected color matching on discolored teeth either before or after the whitening, the impact of the whitening on the color of the resin composite alone may be negligible.</p>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luis Jané-Chimeno DDS, MS, Alfonso Gil DDS, MS, PhD, Rafael Jaen-Gutiérrez CDT, Patricia Freire DDS, MS, Adela Jané-Chimeno DDS, Gerardo Ruales-Suárez DDS, MSc, PhD
Objective
In oral rehabilitation, a full mouth minimal invasive treatment can represent a major challenge for the patient and the dentist. The purpose of this article is to present a new technique to restore eroded teeth and recover the vertical dimension with a simple and predictable technique.
Clinical Considerations
Occlusal tabletop restorations are a suitable conservative option to restore anatomy and vertical dimension augmentation but highly sensitive. The cementation of those restorations without stable landmarks, the cement excess removal, the insertion path, or the time needed are some of the difficulties can be faced. Now a days with the new CAD-CAM techniques is possible to develop a new occlusal tabletop manufacturing alternative utilizing 3D-printed technology by unifying the restorations with a customized connector that allows us to print several onlays at same time, as a single object, also being cemented at once.
Conclusions
This protocol reduces the technique sensitivity of a vertical dimension oral rehabilitation process, reduce the chair time, enhancing the patient-comfort and delivers a unique way to restore dental lost anatomy as a definitive or temporary way with printing materials.
Clinical Significance
With the advent of new CAD-CAM 3D printing materials, which are increasingly versatile and gaining favor among clinicians, it is now possible to address complex clinical situations with greater predictability. This technology enables the development of treatment solutions that are both effective and efficient, consequently reducing clinical time for the patient.
目的：在口腔修复中，全口微创治疗对患者和牙医来说都是一项重大挑战。本文旨在介绍一种新技术，以简单、可预测的技术修复糜烂牙齿并恢复垂直度：临床考虑：咬合台式修复体是恢复解剖结构和增加垂直度的一种合适的保守选择，但非常敏感。在没有稳定地标的情况下粘接这些修复体、去除多余的粘接剂、插入路径或所需时间都是可能面临的一些困难。如今，随着新的 CAD-CAM 技术的发展，我们可以利用 3D 打印技术开发一种新的咬合台面制造替代方法，通过定制的连接器将修复体统一起来，这样我们就可以将多个嵌体作为一个物体同时打印出来，并同时进行粘结：该方案降低了垂直维度口腔修复过程中的技术敏感性，减少了椅上时间，提高了患者舒适度，并提供了一种独特的方法，利用打印材料以最终或临时的方式恢复牙齿缺失的解剖结构：随着新型 CAD-CAM 3D 打印材料的出现，其用途越来越广泛，越来越受到临床医生的青睐，现在有可能以更高的可预测性应对复杂的临床情况。这项技术能够开发出既有效又高效的治疗方案，从而缩短患者的临床治疗时间。
{"title":"New adhesive rehabilitation technique with indexed occlusal tabletops made by 3D printing technology","authors":"Luis Jané-Chimeno DDS, MS, Alfonso Gil DDS, MS, PhD, Rafael Jaen-Gutiérrez CDT, Patricia Freire DDS, MS, Adela Jané-Chimeno DDS, Gerardo Ruales-Suárez DDS, MSc, PhD","doi":"10.1111/jerd.13278","DOIUrl":"10.1111/jerd.13278","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>In oral rehabilitation, a full mouth minimal invasive treatment can represent a major challenge for the patient and the dentist. The purpose of this article is to present a new technique to restore eroded teeth and recover the vertical dimension with a simple and predictable technique.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Considerations</h3>\u0000 \u0000 <p>Occlusal tabletop restorations are a suitable conservative option to restore anatomy and vertical dimension augmentation but highly sensitive. The cementation of those restorations without stable landmarks, the cement excess removal, the insertion path, or the time needed are some of the difficulties can be faced. Now a days with the new CAD-CAM techniques is possible to develop a new occlusal tabletop manufacturing alternative utilizing 3D-printed technology by unifying the restorations with a customized connector that allows us to print several onlays at same time, as a single object, also being cemented at once.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This protocol reduces the technique sensitivity of a vertical dimension oral rehabilitation process, reduce the chair time, enhancing the patient-comfort and delivers a unique way to restore dental lost anatomy as a definitive or temporary way with printing materials.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>With the advent of new CAD-CAM 3D printing materials, which are increasingly versatile and gaining favor among clinicians, it is now possible to address complex clinical situations with greater predictability. This technology enables the development of treatment solutions that are both effective and efficient, consequently reducing clinical time for the patient.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 12","pages":"1614-1622"},"PeriodicalIF":3.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jerd.13278","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liliane Cristina Nogueira Marinho DDS, MS, PhD, Raissa Pinheiro de Paiva DDS, MS, Ana Luísa de Barros Pascoal DDS, MS, PhD, Carlos Augusto Galvão Barboza DDS, MS, PhD, André Ulisses Dantas Batista DS, MS, PhD, Patrícia dos Santos Calderon DDS, MS, PhD
Objective
To evaluate the effect of the deterioration of computer aided design/computer aided manufacturing (CAD/CAM) burs during zirconia milling, on surface roughness, contact angle, and fibroblast viability.
Materials and Methods
Ceramic blocks were milled and 75 ceramic disks (8 × 1.5 mm) made and allocated into three groups (n = 25): G1-brand new 2L and 1L burs, G2-2L bur at the end of lifetime and brand new 1L bur and G3-both burs at the end of their lifetimes. Roughness (Ra, Rq, and Rz) was evaluated using a 3D optical profilometer, the contact angle by the sessile drop method and the cell viability of the mouse NIH/3T3 fibroblast, using the Alamar Blue assay at intervals of 24, 48, and 72 h (ISO 10993-5). Data were analyzed by one-way ANOVA and Kruskal–Wallis tests (p ≤ 0.05).
Results
Roughness increased as the burs deteriorated and G3 (0.27 ± 0.04) presented a higher value for Ra (p < 0.001). The highest contact angle was observed in G3 (86.2 ± 2.66) when compared with G1 (63.7 ± 12.49) and G2 (75.3 ± 6.36) (p < 0.001). Alamar Blue indicated an increase in cell proliferation, with no significant differences among the groups at 24 and 72 h (p > 0.05).
Conclusions
The deterioration of the burs increased the surface roughness and decreased the wettability, but did not interfere in cell viability and proliferation.
Clinical Significance
The use of custom zirconia abutments represents an effective strategy for single crowns restorations. Our findings suggest that these abutments can be efficiently milled using CAD/CAM burs within their recommended lifetime.
{"title":"Impact of CAD/CAM burs lifetime on surface properties of dental zirconia: Implications for biocompatibility of custom abutments","authors":"Liliane Cristina Nogueira Marinho DDS, MS, PhD, Raissa Pinheiro de Paiva DDS, MS, Ana Luísa de Barros Pascoal DDS, MS, PhD, Carlos Augusto Galvão Barboza DDS, MS, PhD, André Ulisses Dantas Batista DS, MS, PhD, Patrícia dos Santos Calderon DDS, MS, PhD","doi":"10.1111/jerd.13277","DOIUrl":"10.1111/jerd.13277","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the effect of the deterioration of computer aided design/computer aided manufacturing (CAD/CAM) burs during zirconia milling, on surface roughness, contact angle, and fibroblast viability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Ceramic blocks were milled and 75 ceramic disks (8 × 1.5 mm) made and allocated into three groups (<i>n</i> = 25): G1-brand new 2L and 1L burs, G2-2L bur at the end of lifetime and brand new 1L bur and G3-both burs at the end of their lifetimes. Roughness (Ra, Rq, and Rz) was evaluated using a 3D optical profilometer, the contact angle by the sessile drop method and the cell viability of the mouse NIH/3T3 fibroblast, using the Alamar Blue assay at intervals of 24, 48, and 72 h (ISO 10993-5). Data were analyzed by one-way ANOVA and Kruskal–Wallis tests (<i>p</i> ≤ 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Roughness increased as the burs deteriorated and G3 (0.27 ± 0.04) presented a higher value for Ra (<i>p</i> < 0.001). The highest contact angle was observed in G3 (86.2 ± 2.66) when compared with G1 (63.7 ± 12.49) and G2 (75.3 ± 6.36) (<i>p</i> < 0.001). Alamar Blue indicated an increase in cell proliferation, with no significant differences among the groups at 24 and 72 h (<i>p</i> > 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The deterioration of the burs increased the surface roughness and decreased the wettability, but did not interfere in cell viability and proliferation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>The use of custom zirconia abutments represents an effective strategy for single crowns restorations. Our findings suggest that these abutments can be efficiently milled using CAD/CAM burs within their recommended lifetime.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 10","pages":"1444-1451"},"PeriodicalIF":3.2,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Panagiotis Ntovas DDS, MSc, Simonas Grybauskas DDS, MD, PhD, Fabian M Beiglboeck MD, DDS, Ziad Kalash MD, Seyidaliyeva Aida DDS, MSc, Wael Att DDS, Dr Med Dent, DMD, PhD
Objective
The aim of the present study was to provide recommendations in order to facilitate communication between dental professionals and surgeons who are collaborating in the field of dentofacial esthetics.
Clinical Considerations
Smile esthetics are beyond the scope, both of the surgeons who are collaborating with facial esthetics and of the dentists, as a wide range of treatment options from both sides is available. It can be difficult for the surgeon or the dentist that first comes in contact with the patient to conduct an individualized global treatment plan, in order to find out how the various phases of the treatment can be sequenced, as a workflow for an efficient interaction between facial surgery and dentistry still does not exist in the scientific literature.
Conclusions
Facial cosmetic procedures and dental treatment have to be planned as individual elements of the whole dentofacial esthetic rehabilitation. The treatment has to be initiated with the design of the smile and the intraoral mock-up, followed by the required surgical interventions, and to be finished with the delivery of the definitive dental restoration.
Clinical Significance
Dentofacial esthetics require comprehensive communication between surgeons and dentists. Following the proposed recommendations, an individualized interdisciplinary treatment plan can be conducted, defining the role of each specialty.
{"title":"What comes first: teeth or face? Recommendations for an interdisciplinary collaboration between facial esthetic surgery and dentistry","authors":"Panagiotis Ntovas DDS, MSc, Simonas Grybauskas DDS, MD, PhD, Fabian M Beiglboeck MD, DDS, Ziad Kalash MD, Seyidaliyeva Aida DDS, MSc, Wael Att DDS, Dr Med Dent, DMD, PhD","doi":"10.1111/jerd.13267","DOIUrl":"10.1111/jerd.13267","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The aim of the present study was to provide recommendations in order to facilitate communication between dental professionals and surgeons who are collaborating in the field of dentofacial esthetics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Considerations</h3>\u0000 \u0000 <p>Smile esthetics are beyond the scope, both of the surgeons who are collaborating with facial esthetics and of the dentists, as a wide range of treatment options from both sides is available. It can be difficult for the surgeon or the dentist that first comes in contact with the patient to conduct an individualized global treatment plan, in order to find out how the various phases of the treatment can be sequenced, as a workflow for an efficient interaction between facial surgery and dentistry still does not exist in the scientific literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Facial cosmetic procedures and dental treatment have to be planned as individual elements of the whole dentofacial esthetic rehabilitation. The treatment has to be initiated with the design of the smile and the intraoral mock-up, followed by the required surgical interventions, and to be finished with the delivery of the definitive dental restoration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>Dentofacial esthetics require comprehensive communication between surgeons and dentists. Following the proposed recommendations, an individualized interdisciplinary treatment plan can be conducted, defining the role of each specialty.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 11","pages":"1489-1501"},"PeriodicalIF":3.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of the study was to investigate the influence of different preparation designs and scan patterns on the accuracy of intraoral scans for complete-arch maxillary laminate veneers.
Materials and Methods
Three maxillary typodonts were used to obtain reference models with three different laminate veneer preparation designs: windows (W), beveled (B), and incisal overlap (IO). Reference scans were obtained with a desktop scanner. A total of 90 complete arch intraoral scans were made with an intraoral scanner (Medit i700) following three different scan patterns: straight motion (SM), zigzag motion (ZM), and combined motion (CM). Ten scans were made in each subgroup and exported as standard tessellation language (STL) files. Assessment of accuracy was conducted with a 3D software analysis program (Geomagic Control X). Each STL file was individually aligned with the reference scan using the best fit algorithm tool, and 3D differences were calculated using the root mean square (RMS) value. Two-way analysis of variance (ANOVA) followed by post hoc comparison tests were applied to analyze precision and trueness data (α = 0.05).
Results
Two-way ANOVA and post hoc comparison tests revealed significant differences among different preparation designs and scan patterns (p < 0.05). Regarding trueness, the IO when scanned with SM presented higher mean RMS than the other preparation designs (W and B) scanned with the same scanning pattern (p < 0.05). Regarding precision, the groups of W and IO presented significantly higher mean RMS than the group of B when scanned with ZM (p < 0.05).
Conclusions
Accuracy of intraoral scans for complete-arch laminate veneers was affected by different laminate veneer preparation designs and scan patterns.
Clinical Significance
Modifying scan pattern according to preparation design helps to improve scan accuracy for complete-arch laminate veneers.
{"title":"Effect of preparation design and scan pattern on the accuracy of intraoral scans for complete arch laminate veneers under simulated intraoral variables","authors":"Nazmiye Şen DDS, PhD, Hatice Eroglu DDS","doi":"10.1111/jerd.13276","DOIUrl":"10.1111/jerd.13276","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>The purpose of the study was to investigate the influence of different preparation designs and scan patterns on the accuracy of intraoral scans for complete-arch maxillary laminate veneers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Three maxillary typodonts were used to obtain reference models with three different laminate veneer preparation designs: windows (W), beveled (B), and incisal overlap (IO). Reference scans were obtained with a desktop scanner. A total of 90 complete arch intraoral scans were made with an intraoral scanner (Medit i700) following three different scan patterns: straight motion (SM), zigzag motion (ZM), and combined motion (CM). Ten scans were made in each subgroup and exported as standard tessellation language (STL) files. Assessment of accuracy was conducted with a 3D software analysis program (Geomagic Control X). Each STL file was individually aligned with the reference scan using the best fit algorithm tool, and 3D differences were calculated using the root mean square (RMS) value. Two-way analysis of variance (ANOVA) followed by post hoc comparison tests were applied to analyze precision and trueness data (<i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Two-way ANOVA and post hoc comparison tests revealed significant differences among different preparation designs and scan patterns (<i>p</i> < 0.05). Regarding trueness, the IO when scanned with SM presented higher mean RMS than the other preparation designs (W and B) scanned with the same scanning pattern (<i>p</i> < 0.05). Regarding precision, the groups of W and IO presented significantly higher mean RMS than the group of B when scanned with ZM (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Accuracy of intraoral scans for complete-arch laminate veneers was affected by different laminate veneer preparation designs and scan patterns.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>Modifying scan pattern according to preparation design helps to improve scan accuracy for complete-arch laminate veneers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 10","pages":"1437-1443"},"PeriodicalIF":3.2,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jerd.13276","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikita Puri BSc, DDS, Pablo J. Atria DDS, MS, PhD, Anabella Oquendo DDS, Markus B. Blatz DMD, PhD, Camila S. Sampaio DDS, MS, PhD
Objective
The present clinical report describes a long-term temporary restorative approach using injectable composite to reshape a canine into a central incisor, within the context of an ongoing orthodontic treatment. This treatment protocol describes a fully-digital workflow, incorporating digital designed and 3D printed diagnostic wax up, reduction guides and resin-injection index.
Clinical Considerations
Effective planning is important when aiming to deliver a comprehensive and multidisciplinary workflow, and digital tools serve as invaluable aids.
Conclusion
The use of a fully digital workflow in a comprehensive dental treatment resulted in a predictable and successful outcome for a restoration of a canine that was transformed into a central incisor.
Clinical Significance
This approach highlights the efficacy of digital technology in achieving precise and successful dental restorations, emphasizing its significance in modern dental practice.
{"title":"A fully digital workflow involving 3D printed tooth reduction guides and injection resin-indexes to restore an impacted canine","authors":"Nikita Puri BSc, DDS, Pablo J. Atria DDS, MS, PhD, Anabella Oquendo DDS, Markus B. Blatz DMD, PhD, Camila S. Sampaio DDS, MS, PhD","doi":"10.1111/jerd.13273","DOIUrl":"10.1111/jerd.13273","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The present clinical report describes a long-term temporary restorative approach using injectable composite to reshape a canine into a central incisor, within the context of an ongoing orthodontic treatment. This treatment protocol describes a fully-digital workflow, incorporating digital designed and 3D printed diagnostic wax up, reduction guides and resin-injection index.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Considerations</h3>\u0000 \u0000 <p>Effective planning is important when aiming to deliver a comprehensive and multidisciplinary workflow, and digital tools serve as invaluable aids.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The use of a fully digital workflow in a comprehensive dental treatment resulted in a predictable and successful outcome for a restoration of a canine that was transformed into a central incisor.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>This approach highlights the efficacy of digital technology in achieving precise and successful dental restorations, emphasizing its significance in modern dental practice.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 10","pages":"1388-1395"},"PeriodicalIF":3.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<div> <section> <h3> Introduction</h3> <p>Erbium-doped yttrium-aluminum-garnet (Er:YAG) laser debonding of zirconia and lithium disilicate restorations is increasingly used for a range of clinical applications. Using rotary instruments to remove such restorations for any purpose has proven to be challenging. Erbium laser has been reported to be a conservative method for removing ceramic restorations. There is little data in the literature about the effect of adhesive resin cement type on the debonding time of the ceramic restoration using the Er:YAG laser.</p> </section> <section> <h3> Objectives</h3> <p>To evaluate and compare the time required for the Er:YAG laser to debond zirconia and lithium disilicate crowns bonded with a 2- and 1-bottle adhesive resin cement systems.</p> </section> <section> <h3> Materials and Methods</h3> <p>Forty extracted premolar teeth were prepared and scanned for milled 40 CAD/CAM crowns. Teeth were randomly assigned into groups (<i>n</i> = 10 per group): 3 mol% yttria-partially stabilized zirconia crowns 3Y-PSZ (G1a) bonded with Panavia™ V5 (2-bottle adhesive resin cement), Zirconia 3Y-PSZ crowns (G1b) bonded with RelyX™ Ultimate (1-bottle adhesive resin cement), and for the lithium disilicate crowns bonded with the two types of cements (G2a, G2b). Each specimen was irradiated with an Er:YAG laser at 335 mJ, 15 Hz, 5.0 W, and 50-ms pulse duration (super short pulse mode). The irradiation time required for crowns to be successfully debonded was recorded for each specimen. Data were statistically analyzed using ANOVA and Tukey HSD post-hoc test (<i>p</i> < 0.05), at the 95 percent level of confidence. The intaglio surface of the debonded crown was analyzed using scanning electron microscopy (SEM).</p> </section> <section> <h3> Results</h3> <p>The mean ± standard deviation times needed for crown debonding were 5.75 ± 2.00 min for the G1a group, 4.79 ± 1.20 min for group G1b, 1.69 ± 0.49 min for group G2a, and 1.12 ± 0.17 for group G2b. There was no statistically significant difference in debonding time between the 2- and 1- bottle adhesive resin cement within the groups G1a and b (<i>p</i> = 0.2914), or between groups G2a b (<i>p</i> = 0.7116). A statistically significant difference (<i>p</i> < 0.05) was found between groups G1a and G2a and b and between groups G1b and G2a and b were SEM analysis showed no changes in the microstructure of the ceramic surface after Er:YAG laser irradiation.</p> </section> <section> <h3> Conclusion</h3> <p>Zirconia and lithium disilicate restorations can be debonded using Er:YAG lasers
{"title":"Effect of Er:YAG laser on debonding zirconia and lithium disilicate crowns bonded with 2- and 1-bottle adhesive resin cements","authors":"Sama Suliman DDS, MS, Taiseer Sulaiman DDS, PhD, Janina Golob Deeb DDS, MS, Awab Abdulmajeed DDS, MS, Aous Abdulmajeed DDS, PhD, Timo Närhi DDS, PhD","doi":"10.1111/jerd.13274","DOIUrl":"10.1111/jerd.13274","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Erbium-doped yttrium-aluminum-garnet (Er:YAG) laser debonding of zirconia and lithium disilicate restorations is increasingly used for a range of clinical applications. Using rotary instruments to remove such restorations for any purpose has proven to be challenging. Erbium laser has been reported to be a conservative method for removing ceramic restorations. There is little data in the literature about the effect of adhesive resin cement type on the debonding time of the ceramic restoration using the Er:YAG laser.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To evaluate and compare the time required for the Er:YAG laser to debond zirconia and lithium disilicate crowns bonded with a 2- and 1-bottle adhesive resin cement systems.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Forty extracted premolar teeth were prepared and scanned for milled 40 CAD/CAM crowns. Teeth were randomly assigned into groups (<i>n</i> = 10 per group): 3 mol% yttria-partially stabilized zirconia crowns 3Y-PSZ (G1a) bonded with Panavia™ V5 (2-bottle adhesive resin cement), Zirconia 3Y-PSZ crowns (G1b) bonded with RelyX™ Ultimate (1-bottle adhesive resin cement), and for the lithium disilicate crowns bonded with the two types of cements (G2a, G2b). Each specimen was irradiated with an Er:YAG laser at 335 mJ, 15 Hz, 5.0 W, and 50-ms pulse duration (super short pulse mode). The irradiation time required for crowns to be successfully debonded was recorded for each specimen. Data were statistically analyzed using ANOVA and Tukey HSD post-hoc test (<i>p</i> < 0.05), at the 95 percent level of confidence. The intaglio surface of the debonded crown was analyzed using scanning electron microscopy (SEM).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean ± standard deviation times needed for crown debonding were 5.75 ± 2.00 min for the G1a group, 4.79 ± 1.20 min for group G1b, 1.69 ± 0.49 min for group G2a, and 1.12 ± 0.17 for group G2b. There was no statistically significant difference in debonding time between the 2- and 1- bottle adhesive resin cement within the groups G1a and b (<i>p</i> = 0.2914), or between groups G2a b (<i>p</i> = 0.7116). A statistically significant difference (<i>p</i> < 0.05) was found between groups G1a and G2a and b and between groups G1b and G2a and b were SEM analysis showed no changes in the microstructure of the ceramic surface after Er:YAG laser irradiation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Zirconia and lithium disilicate restorations can be debonded using Er:YAG lasers ","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 12","pages":"1687-1692"},"PeriodicalIF":3.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camila Sales Jreige DDS, MS, Rafael Gama DDS, Hian Parize DDS, MS, Gokcen Ates DDS, PhD, Newton Sesma DDS, PhD
Objective
The aim of this report is to present the complete workflow of 3D virtual patient for planning and performing implant surgery with magnetically retained 3D-printed stackable guides.
Clinical Considerations
A 3D-printed stackable system was proposed based on bone, dental, and facial references. Initially, a 66-year-old male patient was digitalized through photographs, cone beam computed tomography, and intraoral scans (Virtuo Vivo, Straumann). All files were merged to create a 3D virtual patient in the planning software (coDiagnostiX, Straumann). Sequential stackable guides were designed, printed, and cured. Magnets were inserted into connectors, and the interim protheses received color characterization. Four mounted guides were produced for the specific purposes of pin fixation, bone reduction, implant placement, and immediate provisionalization. After surgery and healing period, patient digital data were updated. Final implant positions were compared to planned values and inconsistencies were clinically acceptable. The mean angular deviation was 5.4° (3.2–7.3) and mean 3D discrepancies were of 0.90 mm (0.46–1.12) at the entry point and 1.68 mm (1.00–2.20) at implant apex. Case follow-up revealed stability, patient's comfort, and no intercurrences.
Conclusion
Magnetically retained stackable guides provide treatment accuracy and reduce surgical and prosthetic complications. The projected virtual patient enhances decision-making and communication between the multidisciplinary team and the patient, while decreases time and costs.
Clinical Significance
Bidimensional diagnosis and freehand implant placement have limitations and outcomes often rely on professionals' expertise. Performing facially driven virtual planning improves treatment predictability. This approach promotes function, esthetic harmony, and patient satisfaction. Implant guided surgery and 3D printed prostheses constitute a reproducible digital workflow that can be implemented into clinical practice to optimize dental care.
目的：本报告旨在介绍三维虚拟患者的完整工作流程，以便使用磁性固定的三维打印可堆叠导板规划和实施种植手术：临床考虑：根据骨骼、牙齿和面部参考资料，提出了一种三维打印可堆叠系统。首先，通过照片、锥形束计算机断层扫描和口内扫描（Virtuo Vivo，Straumann）对一名 66 岁的男性患者进行数字化处理。合并所有文件后，在规划软件（coDiagnostiX，Straumann）中创建一个三维虚拟患者。设计、打印和固化顺序可堆叠导板。将磁铁插入连接器，并对临时假体进行颜色鉴定。制作了四个安装好的导板，分别用于针固定、骨缩小、种植体植入和即刻临时修复。手术和愈合期结束后，对患者的数字数据进行更新。将最终的种植体位置与计划值进行比较，不一致的地方在临床上是可以接受的。平均角度偏差为 5.4° (3.2-7.3)，平均三维偏差为入口处 0.90 mm (0.46-1.12)，种植体顶点 1.68 mm (1.00-2.20)。病例随访结果显示，种植体稳定，患者舒适，未发现穿插感染：结论：磁力固位的可堆叠导板可以提供准确的治疗，减少手术和修复并发症。投影虚拟患者增强了多学科团队与患者之间的决策和沟通，同时减少了时间和成本：临床意义：双维诊断和徒手植入种植体有其局限性，结果往往取决于专业人员的专业知识。进行面部驱动的虚拟规划可以提高治疗的可预测性。这种方法可提高功能、美学和谐度和患者满意度。种植引导手术和三维打印修复体构成了可复制的数字化工作流程，可应用于临床实践，优化牙科护理。
{"title":"3D virtual patient—Magnetically retained printed stackable system for implant guided placement: Case report","authors":"Camila Sales Jreige DDS, MS, Rafael Gama DDS, Hian Parize DDS, MS, Gokcen Ates DDS, PhD, Newton Sesma DDS, PhD","doi":"10.1111/jerd.13265","DOIUrl":"10.1111/jerd.13265","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The aim of this report is to present the complete workflow of 3D virtual patient for planning and performing implant surgery with magnetically retained 3D-printed stackable guides.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Considerations</h3>\u0000 \u0000 <p>A 3D-printed stackable system was proposed based on bone, dental, and facial references. Initially, a 66-year-old male patient was digitalized through photographs, cone beam computed tomography, and intraoral scans (Virtuo Vivo, Straumann). All files were merged to create a 3D virtual patient in the planning software (coDiagnostiX, Straumann). Sequential stackable guides were designed, printed, and cured. Magnets were inserted into connectors, and the interim protheses received color characterization. Four mounted guides were produced for the specific purposes of pin fixation, bone reduction, implant placement, and immediate provisionalization. After surgery and healing period, patient digital data were updated. Final implant positions were compared to planned values and inconsistencies were clinically acceptable. The mean angular deviation was 5.4° (3.2–7.3) and mean 3D discrepancies were of 0.90 mm (0.46–1.12) at the entry point and 1.68 mm (1.00–2.20) at implant apex. Case follow-up revealed stability, patient's comfort, and no intercurrences.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Magnetically retained stackable guides provide treatment accuracy and reduce surgical and prosthetic complications. The projected virtual patient enhances decision-making and communication between the multidisciplinary team and the patient, while decreases time and costs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>Bidimensional diagnosis and freehand implant placement have limitations and outcomes often rely on professionals' expertise. Performing facially driven virtual planning improves treatment predictability. This approach promotes function, esthetic harmony, and patient satisfaction. Implant guided surgery and 3D printed prostheses constitute a reproducible digital workflow that can be implemented into clinical practice to optimize dental care.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 12","pages":"1604-1613"},"PeriodicalIF":3.2,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To evaluate the influence of air-abrasion of enamel with three different desensitizing powders on the whitening effect of a bleaching gel containing 40% H2O2, which was used for in-office tooth bleaching.
Materials and Methods
Forty human incisors, extracted and prepared, were acquired for this study and subsequently randomized into four groups (n = 10). The control group specimens underwent no pretreatment prior to the bleaching procedure, whereas the remaining three groups underwent air abrasion using distinct desensitizing powders; (a) Sylc, which contains bioglass 45S5; (b) BioMinF, which contains calcium phospho-fluoro-silicate glass; and (c) MI Pearls, which contains nano-hydroxyapatite, 1 h preceding the Opalescence Boost PF 40% bleaching procedure. Color measurements were conducted using a double-beam UV–Vis spectrophotometer at four distinct time points (prior to bleaching, 24 h, 15 days, and 30 days post-bleaching).
Results
Tooth color change outcomes revealed that there were no statistically significant results with respect to the interaction of the two criteria (treatments and time) (p = 0.990). Additionally, there were no statistically significant results with respect to the main effects of treatments (p = 0.385), while there were statistically significant effects with respect to the time criterion (p = 0.013).
Conclusions
The use of the tested desensitizing powders prior the bleaching procedure did not affect the tooth color change induced by the tested bleaching agent.
Clinical Significance
Tooth color change and whiteness are not affected by air-abrasion desensitizing treatments when applied prior to in-office bleaching procedures.
{"title":"Effect of air-abrasion pretreatment with three desensitizing agents on efficacy of in-office tooth bleaching","authors":"Ioannis Memis MSc, DDS, Dimitrios Dionysopoulos PhD, MSc, DDS, Constantinos Papadopoulos PhD, MSc, DDS, Petros Mourouzis PhD, MSc, DDS, Sotiria Davidopoulou PhD, MSc, DDS, Kosmas Tolidis PhD, MSc, DDS","doi":"10.1111/jerd.13272","DOIUrl":"10.1111/jerd.13272","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the influence of air-abrasion of enamel with three different desensitizing powders on the whitening effect of a bleaching gel containing 40% H<sub>2</sub>O<sub>2</sub>, which was used for in-office tooth bleaching.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Forty human incisors, extracted and prepared, were acquired for this study and subsequently randomized into four groups (<i>n</i> = 10). The control group specimens underwent no pretreatment prior to the bleaching procedure, whereas the remaining three groups underwent air abrasion using distinct desensitizing powders; (a) Sylc, which contains bioglass 45S5; (b) BioMinF, which contains calcium phospho-fluoro-silicate glass; and (c) MI Pearls, which contains nano-hydroxyapatite, 1 h preceding the Opalescence Boost PF 40% bleaching procedure. Color measurements were conducted using a double-beam UV–Vis spectrophotometer at four distinct time points (prior to bleaching, 24 h, 15 days, and 30 days post-bleaching).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Tooth color change outcomes revealed that there were no statistically significant results with respect to the interaction of the two criteria (treatments and time) (<i>p</i> = 0.990). Additionally, there were no statistically significant results with respect to the main effects of treatments (<i>p</i> = 0.385), while there were statistically significant effects with respect to the time criterion (<i>p</i> = 0.013).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of the tested desensitizing powders prior the bleaching procedure did not affect the tooth color change induced by the tested bleaching agent.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>Tooth color change and whiteness are not affected by air-abrasion desensitizing treatments when applied prior to in-office bleaching procedures.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 10","pages":"1426-1436"},"PeriodicalIF":3.2,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jerd.13272","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rafael Dascanio DDS, MS, Rafael Antonio de Oliveira Ribeiro DDS, MS, Camila Siqueira Silva Coelho DDS, MS, PhD, Marina Trevelin Souza DDS, MS, PhD, Matheus Kury DDS, MS, PhD, Edgar Dutra Zanotto Eng, MS, PhD, Carlos Alberto de Souza Costa DDS, MS, PhD, Vanessa Cavalli DDS, MS, PhD
Aim
This study evaluated the efficacy and cytotoxicity of 35% hydrogen peroxide (HP) gel incorporated with 10% (w/w) biosilicate (BioS) on sound enamel and early-stage enamel erosion lesions.
Methods
Discs of enamel/dentin were selected, subjected to erosive cycles (0.3% citric acid, pH 2.6), and treated with (n = 8): HP (35% HP, positive control); HP_BioS [carboxymethyl cellulose (CMC) + HP + BioS]; BioS (CMC + BioS); CMC (negative control). The discs were adapted to artificial pulp chambers with the enamel exposed for bleaching, and the dentin facing toward the culture medium (Dulbecco's modified Eagle's medium [DMEM]). Bleaching was performed in three 30-min sessions at 7-day intervals. After bleaching, the diffusion product (DMEM extract + diffused HP) was pipetted onto MDPC-23 odontoblastic cell line and inoculated. Color parameters (ΔL, Δa, Δb), color change (ΔE00), and changes in whiteness index (ΔWID) were determined before (T0) and after the last bleaching session (T3). Cell viability (MTT, %), H2O2 diffusion (μg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, in confocal microscopy) were assessed (ANOVA/Tukey; α = 0.05).
Results
No difference in ΔL, Δa, Δb, ΔE00, and ΔWID were found between HP and HP_BioS (p > 0.05). The incorporation of BioS decreased the HP diffusion into the substrates and mitigated oxidative stress in early-stage eroded enamel (p < 0.05). HP_BioS presented significantly higher cell viability compared with HP under erosion conditions. Live/dead assay indicated that BioS_HP maintained viability with larger clusters of viable cells.
Conclusion
Incorporating BioS into HP maintained bleaching effectiveness, favored cell viability, reduced the oxidative stress, and the cytotoxicity in teeth with early-stage erosion.
Clinical Significance
BioS formulation showed promising results for reducing cytotoxicity in patients seeking tooth bleaching and presenting undetectable early-stage erosion.
{"title":"Effectiveness and safety of biosilicate-enhanced bleaching gels on enamel with early erosion lesion","authors":"Rafael Dascanio DDS, MS, Rafael Antonio de Oliveira Ribeiro DDS, MS, Camila Siqueira Silva Coelho DDS, MS, PhD, Marina Trevelin Souza DDS, MS, PhD, Matheus Kury DDS, MS, PhD, Edgar Dutra Zanotto Eng, MS, PhD, Carlos Alberto de Souza Costa DDS, MS, PhD, Vanessa Cavalli DDS, MS, PhD","doi":"10.1111/jerd.13271","DOIUrl":"10.1111/jerd.13271","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This study evaluated the efficacy and cytotoxicity of 35% hydrogen peroxide (HP) gel incorporated with 10% (w/w) biosilicate (BioS) on sound enamel and early-stage enamel erosion lesions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Discs of enamel/dentin were selected, subjected to erosive cycles (0.3% citric acid, pH 2.6), and treated with (<i>n</i> = 8): HP (35% HP, positive control); HP_BioS [carboxymethyl cellulose (CMC) + HP + BioS]; BioS (CMC + BioS); CMC (negative control). The discs were adapted to artificial pulp chambers with the enamel exposed for bleaching, and the dentin facing toward the culture medium (Dulbecco's modified Eagle's medium [DMEM]). Bleaching was performed in three 30-min sessions at 7-day intervals. After bleaching, the diffusion product (DMEM extract + diffused HP) was pipetted onto MDPC-23 odontoblastic cell line and inoculated. Color parameters (Δ<i>L</i>, Δ<i>a</i>, Δ<i>b</i>), color change (Δ<i>E</i><sub>00</sub>), and changes in whiteness index (ΔWI<sub>D</sub>) were determined before (<i>T</i><sub>0</sub>) and after the last bleaching session (<i>T</i><sub>3</sub>). Cell viability (MTT, %), H<sub>2</sub>O<sub>2</sub> diffusion (μg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, in confocal microscopy) were assessed (ANOVA/Tukey; <i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>No difference in Δ<i>L</i>, Δ<i>a</i>, Δ<i>b</i>, Δ<i>E</i><sub>00</sub>, and ΔWI<sub>D</sub> were found between HP and HP_BioS (<i>p</i> > 0.05). The incorporation of BioS decreased the HP diffusion into the substrates and mitigated oxidative stress in early-stage eroded enamel (<i>p</i> < 0.05). HP_BioS presented significantly higher cell viability compared with HP under erosion conditions. Live/dead assay indicated that BioS_HP maintained viability with larger clusters of viable cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Incorporating BioS into HP maintained bleaching effectiveness, favored cell viability, reduced the oxidative stress, and the cytotoxicity in teeth with early-stage erosion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>BioS formulation showed promising results for reducing cytotoxicity in patients seeking tooth bleaching and presenting undetectable early-stage erosion.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":"36 10","pages":"1412-1425"},"PeriodicalIF":3.2,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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