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Rapid recognition of origins and ages of Rice wine based on Tri-step IR and electronic tongue 基于三步红外和电子舌的米酒起源和年代的快速识别
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-06-14 DOI: 10.25177/JFST.3.3.3
Changhua Xu, Yu Yan, Xiao-peng Zhang, Jia-juan Huang, Xiao-zhen Hu, Jian-Hong Gan, Zhengquan Wang, Ningping Tao, Xichang Wang, Sift Desk Journals Open Access Journals
Rice wine, an ancient drink of China, commonly has different origins and wine ages according to traditional processing methods, inducing great challenges to be authenticated and discriminated rapidly by conventional procedures. This study sought to develop an easy and reliable method, based on a Tri-step infrared spectroscopy, combined with electronic sensory for accurate and rapid discrimination of rice wine (RW). The class of nutrition (lipids, protein and other compounds), wine ages and origins were step-wisely evaluated and nutrition profile variations among different samples were integrated and interpreted by fast identification of their relative contents, based on spectral peak intensity. 140 batches of RW (twenty for each samples) were effectively classified by IR-PCA. In the sensory aspect, an electronic tongue distinguished rice wine objectively in taste with discrimination indexes that were greater than 95 for all the samples. The developed method would be an economic and simple approach for rapid chemotaxonomic discrimination of
黄酒是中国的一种古老饮料,根据传统的加工方法,黄酒通常有不同的起源和酒龄,这给传统程序的快速鉴定和鉴别带来了很大的挑战。本研究旨在建立一种简便、可靠的基于三步红外光谱结合电子传感的黄酒鉴别方法。营养成分(脂类、蛋白质和其他化合物)、酒龄和产地被逐步评估,不同样品之间的营养特征变化被整合,并通过基于光谱峰强度的相对含量的快速识别来解释。采用IR-PCA对140批RW(每个样品20批)进行了有效分类。在感官方面,电子舌对黄酒进行了客观的味觉鉴别,所有样品的鉴别指数均大于95。该方法是一种经济、简便的快速化学分类方法
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引用次数: 0
Microbiological analysis of lettuce (Lactuca sativa) from conventional and organic cultivation commercialized at fairs in Brazil 在巴西集市上商业化的传统和有机种植莴苣(Lactuca sativa)的微生物分析
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-06-11 DOI: 10.25177/JFST.3.2.4
T. Gandra, Pâmela Inchauspe Corrêa Alves, E. A. Gandra, Sift Desk Journals Open Access Journals
Brazilian laws established regulatory limits in lettuce only to thermotolerant coliforms (fecal coliforms) and coagulase-positive Staphylococci of 10 MPN g and 10 CFU g respectively. The objective of this study was to evaluate and compare contamination of lettuce from conventional and organic cultivation, commercialized at fairs in south of Brazil, by the fecal coliforms, coagulase-positive Staphylococci, Salmonella spp. and Listeria monocytogenes. Eighty samples of traditionally and organically produced lettuce each were collected. The contaminated samples percent were 35 and 71.25, for fecal coliforms, 35 and 32,5 for coagulase-positive Staphylococci, 0 and 5 for Salmonella spp., 2.5 and 2.5 for Listeria monocytogenes, in conventional lettuce and organic lettuce, respectively. Results indicated that 35% of the samples of conventionally produced lettuce and 71.25% of the samples of organically produced one presented fecal coliform count above regulatory limits. Coagulasepositive Staphylococci counts above regulatory limits were verified in 28 and 26 samples from the conventional and organic agriculture, respectively. Salmonella spp. was present in 5% of organically produced samples and L. monocytogenes in 2.5% of lettuce samples from both types of agricultures. Obtained results highlight the importance of good practices from cultivation to commercialization to improve the hygienic-sanitary quality of vegetables.
巴西法律仅对生菜中的耐热大肠菌群(粪便大肠菌群)和凝固酶阳性葡萄球菌分别制定了10 MPN g和10 CFU g的监管限制。本研究的目的是评估和比较在巴西南部集市上商业化的传统种植和有机种植莴苣的粪便大肠菌群、凝固酶阳性葡萄球菌、沙门氏菌和单核增生李斯特菌的污染情况。收集了80个传统和有机生产的生菜样本。粪大肠菌群、凝固酶阳性葡萄球菌、沙门氏菌、单核增生李斯特菌的污染率分别为35%和71.25、35%和32%、5%、0%和5%,有机生菜和普通生菜的污染率分别为2.5%和2.5%。结果表明,35%的常规生菜和71.25%的有机生菜粪便大肠菌群超标。常规农业和有机农业样品中分别有28份和26份样品的凝固阳性葡萄球菌计数超过规定限值。在这两种类型的农业中,5%的有机生产样品中存在沙门氏菌,2.5%的生菜样品中存在单核细胞增生乳杆菌。获得的结果强调了从种植到商业化的良好实践对提高蔬菜卫生质量的重要性。
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引用次数: 0
Thiol-Cyclodextrin: A New Agent For Controlling The Catalytic Activity Of Polyphenol Oxidase From Red Delicious Apple 巯基环糊精:控制红鲜苹果多酚氧化酶催化活性的新药剂
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-05-04 DOI: 10.25177/jfst.3.2.2
K. Ovsejevi, Gabriela Peralta-Altier, C. Manta, Sift Desk Journals Open Access Journals
Polyphenol oxidase (PPO, EC 1.14.18.1) is the main enzyme responsible for enzymatic browning, a natural process which produces deterioration of fruits and vegetables. One alternative to prevent this undesirable process is to inhibit the catalytic activity of PPO by encapsulating enzyme’s substrates in cyclodextrins (CD). In this article the effect of a Thiol-CD on PPO from Red Delicious apple was studied, demonstrating that this compound is a powerful tool for controlling oxidative processes in food. Thiol-CD could encapsulate the polyphenols, natural substrates of the enzyme, by means of the cyclodextrin hydrophobic internal cavity. Simultaneously, through the thiol group, it could inactivate the PPO by reducing the copper ions from the active site of the enzyme. Moreover, thiol moieties could decrease the browning by reducing the quinones generated by oxidative processes. The isolation and purification of PPO from apple was performed in order to study those effects, different polyphenols, chlorogenic acid (CA) and 4-methylcatechol (4-MC), were assayed as enzymatic substrates. Both β -CD and Thiol-CD exhibited better enzyme inhibition value for CA than for 4-MC. Moreover, Thiol-CD showed an extraordinary performance compared with β-CD. When CA 10 mM was used, 5 mM β-CD gave 11 % PPO inhibition, meanwhile nearly 100 times less concentration of Thiol-CD (45 μM) gave 100 % of enzyme inhibition.
多酚氧化酶(PPO, EC 1.14.18.1)是导致酶促褐变的主要酶,这是一种导致水果和蔬菜变质的自然过程。防止这种不良过程的一种替代方法是通过将酶的底物包封在环糊精(CD)中来抑制PPO的催化活性。本文研究了巯基cd对红美味苹果中PPO的影响,证明该化合物是控制食品氧化过程的有力工具。巯基cd可以通过环糊精疏水内腔包封酶的天然底物多酚。同时,通过巯基,它可以减少酶活性部位的铜离子,使PPO失活。此外,巯基可以通过减少氧化过程中产生的醌来减少褐变。为了研究苹果中PPO的分离纯化作用,以绿原酸(CA)和4-甲基儿茶酚(4-MC)为酶促底物进行了酶促活性测定。β -CD和Thiol-CD对CA的抑制作用均优于4-MC。此外,与β-CD相比,Thiol-CD表现出非凡的性能。当CA浓度为10 mM时,5 mM β-CD对PPO的抑制作用为11%,而低浓度的Thiol-CD (45 μM)对酶的抑制作用为100%。
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引用次数: 1
Suitable preanalytical conditions for vitamin C measurement in clinical routine 临床常规测定维生素C的适宜分析前条件
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-05-03 DOI: 10.25177/JFST.3.2.3
C. Steuer, L. Bernasconi, C. Saxer, P. Neyer, A. Huber, Sift Desk Journals Open Access Journals
Pre-analytical sampling conditions play an important role for the correct determination of blood concentrations of biomarkers and drugs in the clinical environment. In the context of daily clinical routine and clinical studies, oxidative stability in vitro should be carefully considered. Herein we report differences in vitamin C levels under commonly used handling and storage conditions and we propose a simple and efficient sampling protocol. Plasma and serum samples were analyzed by HPLC-UV and different handling-, timeand storage conditions were investigated in detail. Our findings show that protection from oxidation significantly prevented degradation under different storage conditions. The immediate separation of stabilized plasma from blood cells showed a protective effect on vitamin C stability. In addition, long-term storage of vitamin C was positively influenced by addition of protective agents, assuring stability up to six months. Oxidative degradation could affect not only vitamin C but also other small molecule metabolites and should therefore be intensively evaluated before implementation in daily clinical routine as well as in metabolomics studies.
在临床环境中,分析前取样条件对于正确测定生物标志物和药物的血液浓度起着重要作用。在日常临床常规和临床研究的背景下,应仔细考虑体外氧化稳定性。本文报告了在常用处理和储存条件下维生素C水平的差异,并提出了一种简单有效的采样方案。采用高效液相色谱-紫外分光光度法对血浆和血清样品进行分析,并对不同处理、时间和储存条件进行了详细研究。我们的研究结果表明,在不同的储存条件下,氧化保护显著地阻止了降解。从血细胞中立即分离稳定的血浆显示出对维生素C稳定性的保护作用。此外,添加保护剂对维生素C的长期储存有积极影响,可确保长达6个月的稳定性。氧化降解不仅会影响维生素C,还会影响其他小分子代谢物,因此在日常临床常规和代谢组学研究中实施之前应进行深入评估。
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引用次数: 2
The challenge of monitoring the hydrolysis of foods lipids during gastrointestinal digestion 在胃肠道消化过程中监测食物脂质水解的挑战
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-04-19 DOI: 10.25177/jfst.3.1.3
M. Guillén, Bárbara Nieva-Echevarría, E. Goicoechea, Sift Desk Journals Open Access Journals
During the last decade obtaining further knowledge on lipid digestion has become a challenging task in the field of Food Science and Nutrition research. However, the great complexity of this process requires the use of sound, accurate and simple analytical techniques which are able to provide as much information as possible; only thus can a global view and therefore a better understanding of the ongoing process be obtained. This review tackles the advantages and drawbacks of the different methodologies currently employed for this purpose, focusing on a new approach, recently developed and based on Proton Nuclear Magnetic Resonance (H NMR) spectroscopy. This new methodological approach not only provides a great deal of information in a simple, rapid and accurate way, but also overcomes many of the disadvantages of the techniques employed to date. In this sense, H NMR can be considered a very promising alternative for research on lipid digestion, contributing to shed more light on the complex digestion process of lipids and the factors that may affect it.
在过去的十年中,获得关于脂质消化的进一步知识已成为食品科学和营养研究领域的一项具有挑战性的任务。然而,这一过程非常复杂,需要使用健全、准确和简单的分析技术,以便能够提供尽可能多的资料;只有这样才能有一个全局的看法,从而更好地了解正在进行的进程。本文综述了目前用于此目的的不同方法的优点和缺点,重点介绍了最近开发的基于质子核磁共振(H NMR)光谱学的新方法。这种新的方法不仅以一种简单、快速和准确的方式提供了大量的信息,而且还克服了迄今所采用的技术的许多缺点。从这个意义上说,H NMR可以被认为是研究脂质消化的一个非常有前途的替代方法,有助于更多地了解脂质复杂的消化过程及其可能的影响因素。
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引用次数: 0
A colorimetric assay using tetrazolium salts with an electron mediator to evaluate yeast growth in opaque dispersed systems 用四氮唑盐和电子介质评价酵母在不透明分散体系中的生长的比色试验
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-04-11 DOI: 10.25177/JFST.3.1.4
C. A. Campos, Aldana L Zalazar, Malena M González, M. F. Gliemmo, Sift Desk Journals Open Access Journals
Background: Traditional microbiological enumeration techniques such as colony count are time consuming. Visual evaluation of turbidity growth lacks objectivity and precision. On the other hand, the accuracy of the spectrophotometric readings can be limited by media opacity and/or additives. In this context, colorimetric methods based on tetrazolium salts are an alternative to traditional methods due to their ability to generate endpoints based on color change. The objective of this study was to develop a colorimetric assay based on two different tetrazolium salts and an electron mediator in order to replace the conventional method of viable plate count or absorbance for microbial growth detection in opaque dispersed systems. Methods: A color imetr ic method to evaluate yeast growth in broth and opaque dispersed systems using two tetrazolium salts, 2,3,5-triphenyl-2H-tetrazolium chloride (TTC), 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5 -phenyl-2H-tetrazolium chloride (INT) and an electron mediator, 2-methoxy-1,4-Naphthoquinone (MNQ) was developed. In addition, the minimum inhibitory concentrations against Zygosaccharomyces bailii for natamycin and lemongrass essential oil were determined by the proposed method and compared with those obtained using the conventional broth microdilution and plate count methods. Results: Showed that the mixture of INT/MNQ, was more effective than TTC/MNQ. In opaque structure systems, the later showed to be more sensitive than absorbance However, in broth it was as sensitivity as absorbance to detect yeast growth. Besides, the INT/MNQ reagent functioned adequately to determine the minimum inhibitory concentrations of antimicrobials in opaque structured systems. Conclusions: These results demonstrated that the proposed method functioned adequately to determine the minimum inhibitory concentrations of antimicrobials in opaque structured systems showing an excellent agreement with the plate count method and the proposed technique allowed the evaluation of yeast growth with lower population levels than the ones required through absorbance readings. Moreover, this colorimetric method is easy to perform and less time consuming.
背景:传统的微生物计数技术如菌落计数是费时的。浊度增长目视评价缺乏客观性和准确性。另一方面,分光光度法读数的准确性可能受到介质不透明度和/或添加剂的限制。在这种情况下,基于四氮唑盐的比色法是传统方法的替代方法,因为它们能够根据颜色变化产生端点。本研究的目的是开发一种基于两种不同的四氮唑盐和电子介质的比色测定方法,以取代传统的活菌板计数或吸光度方法,用于不透明分散系统中的微生物生长检测。方法:采用2,3,5-三苯基- 2h -四氯化氮(TTC)、2-(4-碘苯基)-3-(4-硝基苯基)-5 -苯基- 2h -四氯化氮(INT)和电子介质2-甲氧基-1,4-萘醌(MNQ)两种四唑盐,建立了在肉汤和不透明分散体系中评价酵母生长的显色法。此外,用该方法测定了纳他霉素和柠檬草精油对白耳酵母的最低抑菌浓度,并与传统的肉汤微量稀释法和平板计数法进行了比较。结果:与TTC/MNQ相比,INT/MNQ的联合用药效果更好。在不透明结构体系中,后者比吸光度更敏感,而在肉汤中,检测酵母生长的灵敏度与吸光度相当。此外,INT/MNQ试剂可以充分确定不透明结构体系中抗菌剂的最低抑制浓度。结论:这些结果表明,所提出的方法能够充分地确定不透明结构体系中抗菌剂的最低抑制浓度,与平板计数法非常一致,并且所提出的技术允许在较低的种群水平下评估酵母生长,而不是通过吸光度读数所需的水平。此外,该比色法操作简单,耗时短。
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引用次数: 2
Replacement meal to treat overweight patients 替代餐治疗超重患者
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-03-15 DOI: 10.25177/JFST.3.1.2
Volpe Roberto, Pacioni Fabrizio, Sorce Maria Luisa, Todisco Miriam, Rome, C. Rome, intern student at Cnr. Vr conception, drafting, Tm, P. A. Moro, Sift Desk Journals Open Access Journals
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引用次数: 0
PLANT DISEASE DETECTION BY IMAGE PROCESSING: A LITERATURE REVIEW 植物病害图像处理检测的文献综述
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.25177/jfst.3.6.6
Monishanker Halder, A. Sarkar, H. Bahar, Sift Desk Journals Open Access Journals
To promote sustainable development, the smart city implies a global vision that merges artificial intelligence, big data, decision making, information and communication technology (ICT), and the Internet-of -Things (IOT). These processes above are related for solving real life problems. Food is one of the basic needs of human being. World population is increasing day by day. So it has become important to grow sufficient amount of crops to feed such a huge population. But with the time passing by, plants are affected with various kinds of diseases, which cause great harm to the agricultural plant productions. Beside that many countries economy greatly depends on agricultural productivity and it’s also a need for a coun try to attain agricultural productivity of basic agricultural product for the people of that particular country. Detection of plant disease through some automatic technique is beneficial as it requires a large amount of work of monitoring in big farm of crops, and at very early stage itself it detects symptoms of diseases means where they appear on plant leaves. In this paper surveys on different disease classification techniques that can be used for plant leaf disease detection.
为了促进可持续发展,智慧城市意味着将人工智能、大数据、决策、信息通信技术(ICT)和物联网(IOT)融合在一起的全球视野。以上这些过程与解决现实生活中的问题有关。食物是人类的基本需求之一。世界人口日益增长。因此,种植足够数量的农作物来养活如此庞大的人口变得非常重要。但随着时间的推移,植物受到各种病害的侵袭,给农业植物生产造成了极大的危害。此外,许多国家的经济在很大程度上依赖于农业生产力,这也是一个国家的基本农产品的农业生产力为特定国家的人民的需要。通过一些自动化技术对植物病害进行检测是有益的,因为它需要在作物大农场中进行大量的监测工作,并且在非常早期的阶段检测病害的症状意味着它们出现在植物叶片上。本文综述了可用于植物叶片病害检测的各种病害分类技术。
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引用次数: 19
柚子精油提取、成分、贮藏及应用研究进展 柚子精油提取、成分、贮藏及应用研究进展
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.3969/j.issn.2095-6002.2018.01.001
陈峰, 孙浩, 倪辉, 洪鹏, 李丽君, 杨远帆, 姜泽东
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引用次数: 0
Evaluation of Enterococcus faecium Ch-1 isolated from Chuli- A traditional fermented apricot product of Trans Himalayan region 跨喜马拉雅地区传统发酵杏产品Chuli中粪肠球菌Ch-1的分离鉴定
IF 3.1 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2017-08-09 DOI: 10.25177/jfst.2.1.4
J. Bajwa, Anupama Sharma, N. Sharma, Sift Desk Journals Open Access Journals
Background: Chuli is a naturally fermented apricot fruit product of Himachal Pradesh and is a rich source of polyphenols and other phytochemicals such as betacarotene and ascorbic acid. In the present research work potential lactic acid bacteria were isolated and explored for its novelty as potential probiotics. Methods: Isolates were screened on the basis of broadest inhibitory spectrum against var ious food borne pathogens i.e , and hence was selected for further study i.e acid and bile tolerance, adherence to gastric mucin sensitivity towards different antibiotics. its relative safety as probiotic candidate was also assessed. Results: Highest antagonism was show by Enterococcus faecium Ch-1 and was found to be tolerant to low pH and high bile concentrations, adherance to gastric mucin. E. faecium Ch-1 was found negative for gelatinase, DNase enzyme activity and haemolysis thus validating its relative safety as probiotic candidate. Conclusion: Enterococcus faecium Ch-1 was found to be a good probiotic strain with cumulative probiotic score of 100% therefore, could be promising for the development as suitable isolate for use in functional foods.
背景:Chuli是喜马偕尔邦天然发酵的杏果产品,富含多酚和其他植物化学物质,如β -胡萝卜素和抗坏血酸。在目前的研究工作中,对乳酸菌作为潜在益生菌的新颖性进行了分离和探索。方法:以对多种食源性致病菌最广泛的抑菌谱为基础,筛选分离菌株进行酸、胆耐受性、胃粘蛋白对不同抗生素敏感性的进一步研究。并对其作为候选益生菌的相对安全性进行了评价。结果:粪肠球菌Ch-1表现出最高的拮抗作用,对低pH和高胆汁浓度具有耐受性,并粘附于胃粘蛋白。大肠杆菌Ch-1明胶酶、脱氧核糖核酸酶和溶血酶活性均为阴性,证实其作为候选益生菌的相对安全性。结论:粪肠球菌Ch-1是一种良好的益生菌菌株,其累积益生菌评分为100%,具有开发应用于功能食品的前景。
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引用次数: 1
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Journal of Food Science and Technology-mysore
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