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Diagnosis of severe anaemia and positive antibody screening as consequences of pre-analytical error. 分析前错误导致的严重贫血诊断和抗体筛查阳性。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-42844
Marta Krygowska-Okrój, Katarzyna Kurpierz, Agnieszka Ćwiklińska

The pre-analytical phase is the principal source of errors in laboratory medicine and continues to pose a challenge to laboratory professionals. We present the case of a 73-yearold female patient with a very low hemoglobin level (69 g/L) and positive indirect antiglobulin test result that indicates the key role of phlebotomy as an important error-prone process in which mistakes can have serious consequences for the patient's diagnosis and treatment. We conclude that there is still an urgent and continuous need to provide educational activities for healthcare professionals involved in blood collection, improve blood collection guideline adherence, and eliminate the errors which can affect diagnosis and treatment, thus jeopardising patient safety.

分析前阶段是实验室医学中错误的主要来源,并继续对实验室专业人员构成挑战。我们报告了一例73岁的女性患者,其血红蛋白水平非常低(69g/L),间接抗球蛋白检测结果呈阳性,这表明静脉切开术作为一个重要的易出错过程的关键作用,在这个过程中,错误可能会对患者的诊断和治疗产生严重后果。我们的结论是,仍然迫切需要持续不断地为参与血液采集的医疗保健专业人员提供教育活动,提高血液采集指南的遵守率,并消除可能影响诊断和治疗的错误,从而危及患者安全。
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引用次数: 0
Analysis of multiple organ function damage in patients with severe COVID-19 pneumonia. 新冠肺炎重症患者多器官功能损害分析。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-41502
Shiyan Feng, Fengxin Wang, Weibo Wu, Yinfeng Li, Chuming Chen, Jianming Li, Mengli Cao, Ling Peng, Peiyan Zhang, Fuxiang Wang

Background: This study aims to analyze the changes and significance of organ function indices in patients with severe Coronavirus Disease 2019 (COVID-19) pneumonia for prediction of major organ damages and guiding treatment schemes.

Methods: 63 patients with severe COVID-19 pneumonia were selected as the severe group and 73 patients with mild syndromes were selected as the mild group. SAS9.4 software was used for statistical analysis of the data.

Results: Levels of ALT, AST, cTnI, Cr, PT, APTT and Ddimer of the severe group were significantly higher while PLT was lower than those of the mild group. The data of all quantitative variables were converted into categorical variables. Significantly higher levels of AST, ALB, D-dimer and higher proportion of bilateral lung involvement were observed from the severe group comparing to those in the mild group, while the difference in the other indices between the two groups was insignificant in statistical perspective.

Conclusions: There are significant differences in the levels of multiple organ function indices between the severe group and the mild group of patients with COVID-19 pneumonia infection. Through examining the relevant indices, conditions of patients' multiple organ function damage could be predicted and used as guidance of treatment.

背景:本研究旨在分析2019年新冠肺炎重症肺炎患者器官功能指标的变化及其意义,以预测主要器官损伤并指导治疗方案。方法:选择63例新冠肺炎重症患者为重症组,73例轻症患者为轻症组。采用SAS9.4软件对数据进行统计分析。结果:重症组ALT、AST、cTnI、Cr、PT、APTT和D二聚体水平明显高于轻症组,PLT低于轻症组。将所有定量变量的数据转换为分类变量。与轻度组相比,重度组的AST、ALB、D-二聚体水平显著升高,双侧肺受累比例也更高,而从统计学角度来看,两组之间其他指标的差异并不显著。结论:新冠肺炎感染者重症组与轻症组多脏器功能指标水平存在显著差异。通过检测相关指标,可以预测患者多器官功能损害的情况,并作为治疗的指导。
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引用次数: 0
The utility of serum amyloid A and other acute-phase reactants determination in ambulatory care COVID-19 patients. 血清淀粉样蛋白A和其他急性相反应物测定在动态护理新冠肺炎患者中的应用。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-42799
Boris Jegorović, Aleksandra Nikolić, Neda Milinković, Svetlana Ignjatović, Sandra Šipetić-Grujičić

Background: The unpredictable course of Coronavirus Disease 19 (COVID-19) is making good severity assessment tools crucial. This study aimed to assess the usefulness of serum amyloid A (SAA) and other acute-phase reactants (APRs) in ambulatory care COVID-19 patients and identified relationships between these markers and disease outcomes.

Methods: From August to November 2020, patients seen in the outpatient department of the Clinic for Infectious and Tropical Diseases (Belgrade, Serbia) with confirmed COVID-19 were included. Patients were classified into mild, moderate, and severe disease groups based on World Health Organization criteria. SAA, C-reactive protein (CRP), interleukin-6 (IL-6), procalcitonin (PCT), ferritin, fibrinogen, D-dimer, albumin, and transferrin were measured. The median values of all APRs were compared between COVID-19 severity groups, hospitalized and non-hospitalized patients, and survivors and non-survivors. The Receiver operator characteristic (ROC) curve analysis was used for the classification characteristics assessment of individual APRs for the severity of illness, hospitalization, and survival.

背景:冠状病毒疾病19(新冠肺炎)的不可预测的过程使得良好的严重性评估工具至关重要。本研究旨在评估血清淀粉样蛋白A(SAA)和其他急性期反应物(APRs)在新冠肺炎门诊患者中的作用,并确定这些标志物与疾病结果之间的关系。方法:纳入2020年8月至11月在传染病和热带病诊所(塞尔维亚贝尔格莱德)门诊就诊的确诊新冠肺炎患者。根据世界卫生组织的标准,患者被分为轻度、中度和重度疾病组。测定SAA、C-反应蛋白(CRP)、白细胞介素-6(IL-6)、降钙素原(PCT)、铁蛋白、纤维蛋白原、D-二聚体、白蛋白和转铁蛋白。比较了新冠肺炎严重程度组、住院和非住院患者、幸存者和非幸存者之间所有APR的中值。受试者-操作者特征(ROC)曲线分析用于评估疾病严重程度、住院和生存率的个体APR的分类特征。
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引用次数: 0
Physiological effect of iron status on patients with polycystic ovary syndrome in Basrah city. 巴士拉市多囊卵巢综合征患者铁状况的生理影响。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-39091a
Dalal F Al-Akabi, Hanadi A Hafth

Polycystic ovary syndrome (PCOS) is one of the most common gynecological diseases that affect the fertility in women in Basra governorate. The current study was designed in order to assess iron aberrations in PCOS patients by measuring the related parameters and their relationship with sex hormones in patients with PCOS. Serum samples were collected from 45 PCOS patients and 45 controls from a private women's clinic and were measured by ELISA in a private medical laboratory. The results showed a significant decrease in the level of hepcidin, transferrin and estradiol versus a significant increase in iron, ferritin, progesterone and testosterone. The current study showed a clear imbalance in the level of iron and its serum regulating parameters in in PCOS women, and there is an effective correlation between iron status and sex hormones.

多囊卵巢综合征(PCOS)是影响巴士拉省妇女生育能力的最常见妇科疾病之一。本研究旨在通过测量多囊卵巢综合征患者的相关参数及其与性激素的关系来评估多囊卵巢综合症患者的铁异常。从一家私人妇女诊所收集了45名多囊卵巢综合征患者和45名对照者的血清样本,并在私人医学实验室通过ELISA进行测量。结果显示,铁调素、转铁蛋白和雌二醇的水平显著降低,而铁、铁蛋白、孕酮和睾酮的水平显著升高。目前的研究表明,多囊卵巢综合征妇女的铁水平及其血清调节参数明显失衡,铁状况与性激素之间存在有效相关性。
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引用次数: 1
Salivary cortisol as a biomarker of stress in surgical patients. 唾液皮质醇作为外科患者压力的生物标志物。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-42011
Sanja Vicković, Ranko Zdravković, Sanja Maričić-Prijić, Dragan Nikolić, Dragana Pap, Emina Čolak, Snežana Jovičić

Background: Surgical stress and pain result in activation of hypothalamus-pituitary-adrenal axis. The aim of this study was to establish the effects of postoperative pain and various modalities of analgesic administration on salivary and serum cortisol levels, as well as to establish the validity of salivary cortisol as a stress indicator in surgical patients.

Methods: A randomized controlled trial involved 60 patients scheduled for elective abdominal aortic aneurysm surgery. Patients were randomly divided into two groups depending on the model of postoperative analgesia. The first group (MI - morphine intermittently) included patients given morphine doses 0.1 mg/kg/6h s.c. intermittently. The second group (MPCA - morphine patient-controlled analgesia) included patients who received morphine via the PCA system - intravenous administration of morphine adjusted to a dose of 1 mg per shot and a lockout interval of 6 minutes.

背景:手术压力和疼痛导致下丘脑-垂体-肾上腺轴的激活。本研究的目的是确定术后疼痛和各种镇痛方式对唾液和血清皮质醇水平的影响,并确定唾液皮质醇作为手术患者压力指标的有效性。方法:一项随机对照试验涉及60名计划接受选择性腹主动脉瘤手术的患者。根据术后镇痛模式将患者随机分为两组。第一组(MI-间歇性吗啡)包括间歇性皮下注射吗啡剂量为0.1mg/kg/6h的患者。第二组(MPCA-吗啡患者控制镇痛)包括通过PCA系统接受吗啡治疗的患者,即静脉注射吗啡,剂量调整为每次1 mg,锁定间隔为6分钟。
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引用次数: 0
Leukocyte cell population data as potential markers of COVID-19 disease characterization. 作为新冠肺炎疾病特征的潜在标志物的白细胞群体数据。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-41589
Giovanni Introcaso, Arianna Galotta, Laura Salvini, Elena Maria Faioni, Alice Bonomi, Emilio Assanelli, Maria Luisa Biondi

Background: The usefulness of leukocyte cell population data (CPD) is currently being investigated. In COVID-19 pandemic several reports showed the clinical importance of hematological parameters. Our study aimed to assess CPDs in Sars CoV-2 patients as new disease markers.

Methods: From February to April 2020 (1st wave) 540 and from September to December 2020 (2nd wave) 2821 patients respectively were enrolled. SARS CoV-2 infection diagnosis was carried out by Multiplex rRT-PCR from nasopharyngeal swabs. CPDs were detected by XN 2000 hematology analyzer (Sysmex Corporation). A comparison between two disease waves was performed. Additionally, C-reactive protein (CRP) and lactate dehydrogenase (LDH) were assayed.

Results: CPDs were classified into: cell complextity, DNA/RNA content and abnormal sized cells. We detected parameters increased from the reference population for all cell types for both 1st and 2nd wave (p<0.05). However, in the 2nd vs 1st wave 5 CPDs vs 9 CPDs were found. In addition we observed higher CPD values of the 1st compared to 2nd wave: (NE-SFL) (p<0.001), (LY-Y) (p<0.0001), (LY-Z) (p<0.0001), (MO-X) (p<0.0001), (MO-Y) (p<0.0001). These findings were confirmed by the higher concentrations of CRP and LDH in the 1st vs 2nd wave: 17.3 mg/L (8.5-59.3) vs 6.3 mg/L (2.3-17.6) (p<0.001) and 241.5 IU/L (201-345) vs 195 IU/L (174-228) (p< 0.001) (median, interquartile range) respectively.

Conclusions: CPDs showed increased cell activation in 1st wave patients confirmed by clinical and biochemical data, associated with worse clinical conditions. Results highlighted the CPDs as disease characterization markers or useful for a risk model.

背景:白细胞群体数据(CPD)的有用性目前正在研究中。在新冠肺炎大流行中,几份报告显示了血液学参数的临床重要性。我们的研究旨在评估严重急性呼吸系统综合征冠状病毒2型患者的CPDs作为新的疾病标志物。方法:2020年2月至4月(第1波)540例,2020年9月至12月(第2波)2821例。通过鼻咽拭子的多重rRT PCR进行SARS冠状病毒2型感染的诊断。通过XN2000血液学分析仪(Sysmex Corporation)检测CPDs。对两种疾病波进行了比较。此外,还测定了C反应蛋白(CRP)和乳酸脱氢酶(LDH)。结果:CPDs可分为:细胞复合体、DNA/RNA含量和异常大小细胞。我们检测到,在第1波和第2波的所有细胞类型中,参数都比参考人群有所增加(P结论:经临床和生化数据证实,CPD显示第1波患者的细胞活化增加,与更糟糕的临床条件有关。结果强调,CPD是疾病表征标志物或可用于风险模型。
{"title":"Leukocyte cell population data as potential markers of COVID-19 disease characterization.","authors":"Giovanni Introcaso,&nbsp;Arianna Galotta,&nbsp;Laura Salvini,&nbsp;Elena Maria Faioni,&nbsp;Alice Bonomi,&nbsp;Emilio Assanelli,&nbsp;Maria Luisa Biondi","doi":"10.5937/jomb0-41589","DOIUrl":"https://doi.org/10.5937/jomb0-41589","url":null,"abstract":"<p><strong>Background: </strong>The usefulness of leukocyte cell population data (CPD) is currently being investigated. In COVID-19 pandemic several reports showed the clinical importance of hematological parameters. Our study aimed to assess CPDs in Sars CoV-2 patients as new disease markers.</p><p><strong>Methods: </strong>From February to April 2020 (1st wave) 540 and from September to December 2020 (2nd wave) 2821 patients respectively were enrolled. SARS CoV-2 infection diagnosis was carried out by Multiplex rRT-PCR from nasopharyngeal swabs. CPDs were detected by XN 2000 hematology analyzer (Sysmex Corporation). A comparison between two disease waves was performed. Additionally, C-reactive protein (CRP) and lactate dehydrogenase (LDH) were assayed.</p><p><strong>Results: </strong>CPDs were classified into: cell complextity, DNA/RNA content and abnormal sized cells. We detected parameters increased from the reference population for all cell types for both 1st and 2nd wave (p<0.05). However, in the 2nd vs 1st wave 5 CPDs vs 9 CPDs were found. In addition we observed higher CPD values of the 1st compared to 2nd wave: (NE-SFL) (p<0.001), (LY-Y) (p<0.0001), (LY-Z) (p<0.0001), (MO-X) (p<0.0001), (MO-Y) (p<0.0001). These findings were confirmed by the higher concentrations of CRP and LDH in the 1st vs 2nd wave: 17.3 mg/L (8.5-59.3) vs 6.3 mg/L (2.3-17.6) (p<0.001) and 241.5 IU/L (201-345) vs 195 IU/L (174-228) (p< 0.001) (median, interquartile range) respectively.</p><p><strong>Conclusions: </strong>CPDs showed increased cell activation in 1st wave patients confirmed by clinical and biochemical data, associated with worse clinical conditions. Results highlighted the CPDs as disease characterization markers or useful for a risk model.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"454-459"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41124983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of obesity on the serum BMP15, GDF9, and kisspeptin concentrations in women of reproductive age. 肥胖对育龄妇女血清BMP15、GDF9和kisspeptin浓度的影响。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-37329
Funda Bulut Arıkan, Nevin Sagsoz

Background: As BMP15, GDF9, and kisspeptin all play critical roles in folliculogenesis and fertilization, investigating the possible relationship between obesity and these three factors could prove crucial in relation to understanding the role of obesity in infertility. Thus, the present study sought to determine the effects of obesity on the serum BMP15, GDF9, and kisspeptin concentrations in women of reproductive age.

Methods: Ninety female participants were equally divided into three groups: class-1 obese (n=30), class-2 obese (n=30), and normal weight (control; n=30). The participants' serum BMP15, GDF9, and AMH concentrations were measured. Moreover, the serum kisspeptin concentrations were evaluated in the class-1 obese and control groups by means of the enzyme-linked immunosorbent assay (ELISA) method while the participants were in their menstrual period.

Results: The serum BMP15 and kisspeptin concentrations were found to be much higher in the control group than in both obese groups (p=0.001 and p=0.01, respectively). While the GDF9 concentration exhibited a statistically significant positive correlation with age, the BMP15 concentration exhibited a positive correlation with the kisspeptin and LH concentrations in the control group. In addition, a positive correlation was identified between the BMP15 concentration and both age and the glucose level and a negative correlation with the insulin level in both the obese groups.

Conclusions: Obesity appears to reduce the serum BMP15 and kisspeptin concentrations in obese women of reproductive age. This reduction may represent a milestone in reproductive dysfunction and may be used to predict the success of infertility treatment in obese women.

背景:由于BMP15、GDF9和kisspeptin都在卵泡发生和受精中发挥着关键作用,研究肥胖与这三个因素之间的可能关系对于理解肥胖在不孕中的作用至关重要。因此,本研究试图确定肥胖对育龄妇女血清BMP15、GDF9和kisspeptin浓度的影响。方法:90名女性参与者被平均分为三组:1级肥胖(n=30)、2级肥胖(n=30)和正常体重(对照组;n=30)。测量参与者的血清BMP15、GDF9和AMH浓度。此外,在参与者月经期间,通过酶联免疫吸附试验(ELISA)方法评估了1类肥胖组和对照组的血清kisspeptin浓度。结果:对照组的血清BMP15和kisspeptin浓度远高于两个肥胖组(分别为p=0.001和p=0.01)。在对照组中,GDF9浓度与年龄呈正相关,BMP15浓度与kisspeptin和LH浓度呈正相关。此外,在两个肥胖组中,BMP15浓度与年龄和葡萄糖水平呈正相关,与胰岛素水平呈负相关。结论:肥胖可降低育龄肥胖妇女的血清BMP15和kisspeptin浓度。这种减少可能代表生殖功能障碍的一个里程碑,并可用于预测肥胖女性不孕治疗的成功。
{"title":"Effects of obesity on the serum BMP15, GDF9, and kisspeptin concentrations in women of reproductive age.","authors":"Funda Bulut Arıkan,&nbsp;Nevin Sagsoz","doi":"10.5937/jomb0-37329","DOIUrl":"10.5937/jomb0-37329","url":null,"abstract":"<p><strong>Background: </strong>As BMP15, GDF9, and kisspeptin all play critical roles in folliculogenesis and fertilization, investigating the possible relationship between obesity and these three factors could prove crucial in relation to understanding the role of obesity in infertility. Thus, the present study sought to determine the effects of obesity on the serum BMP15, GDF9, and kisspeptin concentrations in women of reproductive age.</p><p><strong>Methods: </strong>Ninety female participants were equally divided into three groups: class-1 obese (n=30), class-2 obese (n=30), and normal weight (control; n=30). The participants' serum BMP15, GDF9, and AMH concentrations were measured. Moreover, the serum kisspeptin concentrations were evaluated in the class-1 obese and control groups by means of the enzyme-linked immunosorbent assay (ELISA) method while the participants were in their menstrual period.</p><p><strong>Results: </strong>The serum BMP15 and kisspeptin concentrations were found to be much higher in the control group than in both obese groups (p=0.001 and p=0.01, respectively). While the GDF9 concentration exhibited a statistically significant positive correlation with age, the BMP15 concentration exhibited a positive correlation with the kisspeptin and LH concentrations in the control group. In addition, a positive correlation was identified between the BMP15 concentration and both age and the glucose level and a negative correlation with the insulin level in both the obese groups.</p><p><strong>Conclusions: </strong>Obesity appears to reduce the serum BMP15 and kisspeptin concentrations in obese women of reproductive age. This reduction may represent a milestone in reproductive dysfunction and may be used to predict the success of infertility treatment in obese women.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"392-400"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Short-term outcome of plasma adsorption therapy in amyotrophic lateral sclerosis. 血浆吸附治疗肌萎缩侧索硬化症的短期疗效。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-40631
Bin Li, Wei Zhang, Shaoxin Zhong, Jianyi Pan, Xiaohong Wang, Hequn Zou, Xianrui Dou

Background: To observe the short-term outcome of plasma adsorption PA therapy in amyotrophic lateral sclerosis (ALS).

Methods: 28 cases of als patients were recruited in this study, of which 20 were male and 8 were female with a mean age of 53.21±9.07 years and the average course of 33±23.35 months. The clinical manifestations were limb weakness (N=27), muscular atrophy (N=27), muscular tremor (N=5), dysphagia (N=12) and dysarthria (N=12). The clinical data of the patients recruited were graded by Amyotrophic Lateral Sclerosis Functional Rating Scale Revised (ALSFRSR) : <10 (N=1), 11-20 (N=4), 21-30 (N=6), 31-40 (N=12), >40 (N=5). All patients received PA therapy once a week for three successive times after examining the conditions of blood coagulation and virus infection. PA therapy was supplemented with neurotrophic therapy meanwhile. All patients' clinical manifestations and scores of ALSFRSR before treatment and one week after treatment were evaluated and compared. The levels of serum superoxide dismutase (SOD), interleukin-10 (IL-10), serum creatine kinase (CK) and lactate dehydrogenase (LDH) before and after treatment were compared.

Results: After PA therapy, 14 patients have improved obviously in muscle strength, 4 patients in hypermyotonia partially, 3 patients in muscular tremor, 5 patients in dysarthria, 3 patients in salivation to some extent and 2 patients in swallowing function. The score of ALSFRSR after PA treatment (31.89±10.36) was remarkably higher than that before PA treatment (30.68±10.52) (P<0.01). The levels of SOD (155.10±21.87 IU/L) and IL-10 (138.06±185.88 pg/mL) after PA treatment were significantly higher than the levels before PA treatment (143.08.3±19.16 IU/L and 46.34±75.31 pg/mL, respectively) (P<0.05). The levels of CK (168.86±113.50 IU/L) and LDH (152.07±32.65 IU/L) after PA treatment were significantly lower than the levels before PA treatment (356.68±250.30 IU/L and 181.36±33.74 IU/L respectively) (P<0.01). At the end of follow-up period (November, 2019), five patients died of respiratory failure 16-21 months after PA treatment and two patents died of respiratory infection 15-20 months after PA treatment. 7 patients were still alive. The score of ALSFRS-R of these patients who survived at the end of follow-up (13.00±13.37) were significantly lower than before PA treatment (36.71±8.56) (P<0.05) and after PA treatment (38.14±8.82) (P<0.05).

Conclusions: Plasma adsorption (PA) therapy has shortterm therapeutic effects on als. The effects might be attributed to the anti-oxygen free radical effect by increasing SOD level and the anti-inflammation effect by increasing IL-10 level. As the efficacy of PA therapy was obtained in a small sample size and short follow-up period, the longterm observation of PA efficacy in treating als should be further investigated.

背景:观察血浆吸附PA治疗肌萎缩侧索硬化症(ALS)的近期疗效。方法:本研究招募了28例ALS患者,其中20例为男性,8例为女性,平均年龄53.21±9.07岁,平均病程33±23.35个月。临床表现为肢体无力(N=27)、肌萎缩(N=27,肌震颤(N=5)、吞咽困难(N=12)和构音障碍(N=12。采用肌萎缩侧索硬化症功能评定量表修订版(ALSFRSR):40(N=5)对招募的患者的临床数据进行评分。所有患者在检查凝血和病毒感染情况后,每周接受一次PA治疗,连续三次。PA治疗辅以神经营养治疗。对所有患者治疗前和治疗后一周的临床表现和ALSFRSR评分进行评估和比较。比较治疗前后血清超氧化物歧化酶(SOD)、白细胞介素-10(IL-10)、血清肌酸激酶(CK)和乳酸脱氢酶(LDH)的水平。结果:PA治疗后,14例患者肌力明显改善,4例部分肌强直,3例肌肉震颤,5例构音障碍,3例有一定程度的流涎,2例吞咽功能改善。PA治疗后ALSFRSR评分(31.89±10.36)明显高于PA治疗前(30.68±10.52)(P结论:血浆吸附治疗对als有短期疗效。这种作用可能归因于提高SOD水平的抗氧自由基作用和提高IL-10水平的抗炎作用。由于PA治疗的疗效是在小样本量和短随访期内获得的,因此应进一步研究PA治疗als的长期疗效观察。
{"title":"Short-term outcome of plasma adsorption therapy in amyotrophic lateral sclerosis.","authors":"Bin Li,&nbsp;Wei Zhang,&nbsp;Shaoxin Zhong,&nbsp;Jianyi Pan,&nbsp;Xiaohong Wang,&nbsp;Hequn Zou,&nbsp;Xianrui Dou","doi":"10.5937/jomb0-40631","DOIUrl":"10.5937/jomb0-40631","url":null,"abstract":"<p><strong>Background: </strong>To observe the short-term outcome of plasma adsorption PA therapy in amyotrophic lateral sclerosis (ALS).</p><p><strong>Methods: </strong>28 cases of als patients were recruited in this study, of which 20 were male and 8 were female with a mean age of 53.21±9.07 years and the average course of 33±23.35 months. The clinical manifestations were limb weakness (N=27), muscular atrophy (N=27), muscular tremor (N=5), dysphagia (N=12) and dysarthria (N=12). The clinical data of the patients recruited were graded by Amyotrophic Lateral Sclerosis Functional Rating Scale Revised (ALSFRSR) : <10 (N=1), 11-20 (N=4), 21-30 (N=6), 31-40 (N=12), >40 (N=5). All patients received PA therapy once a week for three successive times after examining the conditions of blood coagulation and virus infection. PA therapy was supplemented with neurotrophic therapy meanwhile. All patients' clinical manifestations and scores of ALSFRSR before treatment and one week after treatment were evaluated and compared. The levels of serum superoxide dismutase (SOD), interleukin-10 (IL-10), serum creatine kinase (CK) and lactate dehydrogenase (LDH) before and after treatment were compared.</p><p><strong>Results: </strong>After PA therapy, 14 patients have improved obviously in muscle strength, 4 patients in hypermyotonia partially, 3 patients in muscular tremor, 5 patients in dysarthria, 3 patients in salivation to some extent and 2 patients in swallowing function. The score of ALSFRSR after PA treatment (31.89±10.36) was remarkably higher than that before PA treatment (30.68±10.52) (P<0.01). The levels of SOD (155.10±21.87 IU/L) and IL-10 (138.06±185.88 pg/mL) after PA treatment were significantly higher than the levels before PA treatment (143.08.3±19.16 IU/L and 46.34±75.31 pg/mL, respectively) (P<0.05). The levels of CK (168.86±113.50 IU/L) and LDH (152.07±32.65 IU/L) after PA treatment were significantly lower than the levels before PA treatment (356.68±250.30 IU/L and 181.36±33.74 IU/L respectively) (P<0.01). At the end of follow-up period (November, 2019), five patients died of respiratory failure 16-21 months after PA treatment and two patents died of respiratory infection 15-20 months after PA treatment. 7 patients were still alive. The score of ALSFRS-R of these patients who survived at the end of follow-up (13.00±13.37) were significantly lower than before PA treatment (36.71±8.56) (P<0.05) and after PA treatment (38.14±8.82) (P<0.05).</p><p><strong>Conclusions: </strong>Plasma adsorption (PA) therapy has shortterm therapeutic effects on als. The effects might be attributed to the anti-oxygen free radical effect by increasing SOD level and the anti-inflammation effect by increasing IL-10 level. As the efficacy of PA therapy was obtained in a small sample size and short follow-up period, the longterm observation of PA efficacy in treating als should be further investigated.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"401-406"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of single-and double-volume exchange transfusion for neonatal hyperbilirubinemia. 单、双容量换血治疗新生儿高胆红素血症的疗效观察。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-42668
Zhongzheng Xiong, Xianchuan Liu, Xiangzhu Li

Background: To investigate the efficacy and safety of singleand double-volume exchange transfusion for neonatal hyperbilirubinemia (HB) and compare their effects on the internal environment of newborns.

Methods: The clinical data of 96 HB newborns admitted to and treated in our hospitals from January 2016 to October 2021 were retrospectively analyzed. Then, these newborns were divided into single volume group (80-110 mL/kg, n=48) and double volume group (150-180 mL/kg, n=48) by the exchange volume per unit body mass. The hematological indicators total serum bilirubin (TSB), peripheral blood red blood cell (RBC) count, white blood cell (WBC) count, platelet (PLT) count, serum albumin (ALB), prothrombin time (PT) and activated partial thromboplastin time (APTT), and changes in inner-environment indexes (blood gas, blood glucose, acid-base and electrolyte levels) were compared between the two groups of newborns before treatment and after once treatment. Additionally, the adverse reactions of exchange transfusion in the two groups of newborns were recorded.

背景:探讨单、双容量换血治疗新生儿高胆红素血症(HB)的疗效和安全性,并比较其对新生儿内环境的影响。方法:对2016年1月至2021年10月我院收治的96例HB新生儿的临床资料进行回顾性分析。然后,按单位体重交换量将新生儿分为单容量组(80~110 mL/kg,n=48)和双容量组(150~180 mL/kg,n=48)。血液学指标血清总胆红素(TSB)、外周血红细胞(RBC)计数、白细胞(WBC)计数、血小板(PLT)计数、血清白蛋白(ALB),比较两组新生儿治疗前后凝血酶原时间(PT)和活化部分凝血活酶时间(APTT)及内环境指标(血气、血糖、酸碱和电解质水平)的变化。此外,还记录了两组新生儿的换血不良反应。
{"title":"Efficacy of single-and double-volume exchange transfusion for neonatal hyperbilirubinemia.","authors":"Zhongzheng Xiong,&nbsp;Xianchuan Liu,&nbsp;Xiangzhu Li","doi":"10.5937/jomb0-42668","DOIUrl":"https://doi.org/10.5937/jomb0-42668","url":null,"abstract":"<p><strong>Background: </strong>To investigate the efficacy and safety of singleand double-volume exchange transfusion for neonatal hyperbilirubinemia (HB) and compare their effects on the internal environment of newborns.</p><p><strong>Methods: </strong>The clinical data of 96 HB newborns admitted to and treated in our hospitals from January 2016 to October 2021 were retrospectively analyzed. Then, these newborns were divided into single volume group (80-110 mL/kg, n=48) and double volume group (150-180 mL/kg, n=48) by the exchange volume per unit body mass. The hematological indicators total serum bilirubin (TSB), peripheral blood red blood cell (RBC) count, white blood cell (WBC) count, platelet (PLT) count, serum albumin (ALB), prothrombin time (PT) and activated partial thromboplastin time (APTT), and changes in inner-environment indexes (blood gas, blood glucose, acid-base and electrolyte levels) were compared between the two groups of newborns before treatment and after once treatment. Additionally, the adverse reactions of exchange transfusion in the two groups of newborns were recorded.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"484-491"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41179110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlation analysis of serum levels of H19 and CRP levels and ulcerative colitis. 血清H19和CRP水平与溃疡性结肠炎的相关性分析。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-41359
Aihua Wang, Yongkang Jiang

Background: To elucidate clinical applications of detecting serum levels of H19 and CRP in predicting the severity of ulcerative colitis (UC).

Methods: Two hundred UC patients were recruited, and classified to mild/moderate group and severe group according to the Truelove-Witts grading system. Serum levels of H19 and CRP in UC patients were detected by turbidimetric inhibition immuno assay and qRT-PCR. Differences in serum levels of H19 and CRP between mild/moderate group and severe group were analyzed. By plotting ROC curves, the diagnostic potentials of H19 and CRP in UC were evaluated. Kappa conformance test was conducted to validate the conformance of detecting serum levels of H19 and CRP to clinical diagnosis of UC.

Results: Serum levels of H19 and CRP were higher in UC patients of severe group than those of mild/moderate group. Their levels were both positively correlated to the severity of UC. High sensitivity (83.3%) and specificity (80.0%), as well as the maximum Youden index (0.633) were obtained at the cut-off value for H19 level of 2.755, and AUC was 0.8835. Meanwhile, Kappa coefficient (k) was 0.760 at the cut-off value for H19 level of 2.755, showing a high conformance to clinical diagnosis of UC. In addition, acceptable sensitivity (68.49%) and high specificity (85.83%), as well as the maximum Youden index (0.543) were obtained at the cut-off value for CRP level of 6.390 mg/L, and AUC was 0.8018. k was 0.435, showing an acceptable conformance to clinical diagnosis of UC based on serum level of CRP.

Conclusions: Serum levels of H19 and CRP increase with the deterioration of UC. Detecting their serum levels has a consistent result to clinical diagnosis of UC, with a superior performance of H19 than that of CRP.

背景:阐明血清H19和CRP水平检测在预测溃疡性结肠炎(UC)严重程度中的临床应用。方法:招募200名UC患者,根据Truelove-Witts评分系统分为轻度/中度组和重度组。采用浊度抑制免疫法和qRT-PCR法检测UC患者血清H19和CRP水平。分析轻度/中度组和重度组之间血清H19和CRP水平的差异。通过绘制ROC曲线,评估H19和CRP在UC中的诊断潜力。采用Kappa一致性试验验证血清H19和CRP水平检测对UC临床诊断的一致性。它们的水平均与UC的严重程度呈正相关。H19水平的临界值为2.755,AUC为0.8835时,获得了高灵敏度(83.3%)和特异性(80.0%),以及最大Youden指数(0.633)。同时,在H19水平的临界值2.755时,Kappa系数(k)为0.760,与UC的临床诊断高度一致。此外,在CRP水平的临界值为6.390 mg/L时,获得了可接受的灵敏度(68.49%)和高特异性(85.83%),以及最大Youden指数(0.543),AUC为0.8018。k为0.435,与基于血清CRP水平的UC临床诊断符合。结论:血清H19和CRP水平随着UC的恶化而升高。检测他们的血清水平与UC的临床诊断结果一致,H19的表现优于CRP。
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引用次数: 1
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Journal of Medical Biochemistry
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