Qin Hu, Yu Mao, Haomiao Lan, Yi Wei, Yuehua Chen, Qiang Ye, Hongying Che
Background: The purpose of this study was to investigate the potential of plasma cfDNA methylation patterns in reflecting tumor methylation changes, focusing on three candidate sites, cg02469161, cg11528914, and cg20131654. These sites were selected for verification, with a particular emphasis on their association with breast cancer. Methods: We conducted a comprehensive analysis of 850k whole-methylation sequencing data to identify potential markers for breast cancer detection. Subsequently, we examined the methylation status of genes RANBP3, LCP2, and GRAP2, where the aforementioned sites are located, using cancer and cancer-adjacent tissues from 17 breast cancer patients. We also assessed the methylation patterns in different molecular subtypes and pathological grades of breast cancer. Additionally, we compared the methylation levels of these genes in plasma cfDNA to their performance in tissues. Results: Our analysis revealed that RANBP3, LCP2, and GRAP2 genes exhibited significant methylation differences between cancer and cancer-adjacent tissues. In breast cancer, these genes displayed diagnostic efficiencies of 91.0%, 90.6%, and 92.2%, respectively. Notably, RANBP3 showed a tendency towards lower methylation in HR+ breast cancer, and LCP2 methylation was correlated with tumor malignancy. Importantly, the methylation levels of these three genes in plasma cfDNA closely mirrored their tissue counterparts, with diagnostic efficiencies of 83.3%, 83.9%, and 77.6% for RANBP3, LCP2, and GRAP2, respectively. Conclusions: Our findings suggest that RANBP3, LCP2, and GRAP2 genes, located at the identified methylation sites, can serve as valuable blood molecular markers for the auxiliary diagnosis of breast cancer. This research provides a foundation for further exploration of gene methylation pattern changes in cfDNA for the detection of breast cancer and other cancer types.
{"title":"Value of altered methylation patterns of genes RANBP3, LCP2 and GRAP2 in cfDNA in breast cancer diagnosis","authors":"Qin Hu, Yu Mao, Haomiao Lan, Yi Wei, Yuehua Chen, Qiang Ye, Hongying Che","doi":"10.5937/jomb0-47507","DOIUrl":"https://doi.org/10.5937/jomb0-47507","url":null,"abstract":"Background: The purpose of this study was to investigate the potential of plasma cfDNA methylation patterns in reflecting tumor methylation changes, focusing on three candidate sites, cg02469161, cg11528914, and cg20131654. These sites were selected for verification, with a particular emphasis on their association with breast cancer. \u0000Methods: We conducted a comprehensive analysis of 850k whole-methylation sequencing data to identify potential markers for breast cancer detection. Subsequently, we examined the methylation status of genes RANBP3, LCP2, and GRAP2, where the aforementioned sites are located, using cancer and cancer-adjacent tissues from 17 breast cancer patients. We also assessed the methylation patterns in different molecular subtypes and pathological grades of breast cancer. Additionally, we compared the methylation levels of these genes in plasma cfDNA to their performance in tissues. \u0000Results: Our analysis revealed that RANBP3, LCP2, and GRAP2 genes exhibited significant methylation differences between cancer and cancer-adjacent tissues. In breast cancer, these genes displayed diagnostic efficiencies of 91.0%, 90.6%, and 92.2%, respectively. Notably, RANBP3 showed a tendency towards lower methylation in HR+ breast cancer, and LCP2 methylation was correlated with tumor malignancy. Importantly, the methylation levels of these three genes in plasma cfDNA closely mirrored their tissue counterparts, with diagnostic efficiencies of 83.3%, 83.9%, and 77.6% for RANBP3, LCP2, and GRAP2, respectively. \u0000Conclusions: Our findings suggest that RANBP3, LCP2, and GRAP2 genes, located at the identified methylation sites, can serve as valuable blood molecular markers for the auxiliary diagnosis of breast cancer. This research provides a foundation for further exploration of gene methylation pattern changes in cfDNA for the detection of breast cancer and other cancer types.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138965065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective:It aims to explore the effect of target task oriented phase training on fibrinogen (Fbg), angiopoietin (Ang-1), vascular endothelial growth factor (VEGF), serum brain derived neurotrophic factor (BDNF) and quality of life in postoperative patients with brain trauma. Methods:142 patients with brain trauma who were operated in neurosurgery of our hospital from March 2020 to March 2023 are chosen and separated into two groups by random number table. The control group (n=71) receive routine postoperative training, and the experimental group (n=71) receive target task oriented training based on the control group, and the serum cell levels of nursing for 3, 7 and 14 days are compared. Improvement of limb function and quality of life after 2, 4, and 6 weeks of nursing care is observed. Results: Before nursing, the comparison in serum factor levels, limb function scores, and quality of life scores between the two groups was with P>0.05; After 3, 7, and 14 days of nursing, the Fbg of the control group was higher than that of the experimental group; The Ang-1, VEGF, and BDNF levels in the experimental group were higher than those in the control group (P<0.05); After 2, 4, and 6 weeks of nursing care, the FMA scores of the upper and lower limbs in the control group were lower than those in the experimental group, with P<0.05; The scores in the physiological, environmental, psychological, and social fields of thecontrol group were lower than those of the experimental group, with P<0.05. Conclusion: The application of target task oriented phase training in patients with brain trauma after surgery can help promote the serum levels of Fbg, Ang-1, VEGF, and BDNF, improve limb function, and enhance quality of life.
{"title":"the The Improvement of Fbg, Ang-1, VEGF, BDNF in Postoperative Patients with Brain Trauma Through Target Task Oriented Phrase Training","authors":"Bo Liu","doi":"10.5937/jomb0-45490","DOIUrl":"https://doi.org/10.5937/jomb0-45490","url":null,"abstract":"Objective:It aims to explore the effect of target task oriented phase training on fibrinogen (Fbg), angiopoietin (Ang-1), vascular endothelial growth factor (VEGF), serum brain derived neurotrophic factor (BDNF) and quality of life in postoperative patients with brain trauma. Methods:142 patients with brain trauma who were operated in neurosurgery of our hospital from March 2020 to March 2023 are chosen and separated into two groups by random number table. The control group (n=71) receive routine postoperative training, and the experimental group (n=71) receive target task oriented training based on the control group, and the serum cell levels of nursing for 3, 7 and 14 days are compared. Improvement of limb function and quality of life after 2, 4, and 6 weeks of nursing care is observed. Results: Before nursing, the comparison in serum factor levels, limb function scores, and quality of life scores between the two groups was with P>0.05; After 3, 7, and 14 days of nursing, the Fbg of the control group was higher than that of the experimental group; The Ang-1, VEGF, and BDNF levels in the experimental group were higher than those in the control group (P<0.05); After 2, 4, and 6 weeks of nursing care, the FMA scores of the upper and lower limbs in the control group were lower than those in the experimental group, with P<0.05; The scores in the physiological, environmental, psychological, and social fields of thecontrol group were lower than those of the experimental group, with P<0.05. Conclusion: The application of target task oriented phase training in patients with brain trauma after surgery can help promote the serum levels of Fbg, Ang-1, VEGF, and BDNF, improve limb function, and enhance quality of life.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The aim of this study was to figure out the predictive value of inflammatory-linked factors on the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children. Methods: Using regression analysis method, 38 children with refractory purulent meningitis, 40 children with purulent meningitis, and 40 healthy children who came to Qingtian County People's Hospital of Lishui City for physical examination during the same period were selected as the research objects, and assigned into the experimental, the control and the healthy groups. The inflammation-linked factors in the three groups were compared, and multivariate Logisitic regression was applied to analyze the predictive indicators and predictive efficacy of dexamethasone treatment in children with refractory suppurative meningitis. Results: CRP, TNF-α, IL-6, PCT and IL-1β were higher in the study group vs. the control and the healthy, and in the control vs. the healthy (P < 0.05). Multivariate Logisitic regression analysis clarified the predictors of the efficacy of dexamethasone treatment in children with refractory suppurative meningitis were CRP, TNF-α, IL-6, PCT, and IL-1β, which all had good predictive performance, and among which CRP and IL-1β had better predictive performance. Conclusion: Inflammatory-linked factors have a certain predictive value for the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children.
{"title":"Predictive value of inflammatory-related factors on the efficacy of adjuvant dexamethasone in the treatment of children with refractory suppurative meningitis","authors":"Kang Xu, XiaoMei Zhong, QingJun Niu","doi":"10.5937/jomb0-37618","DOIUrl":"https://doi.org/10.5937/jomb0-37618","url":null,"abstract":"Purpose: The aim of this study was to figure out the predictive value of inflammatory-linked factors on the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children. Methods: Using regression analysis method, 38 children with refractory purulent meningitis, 40 children with purulent meningitis, and 40 healthy children who came to Qingtian County People's Hospital of Lishui City for physical examination during the same period were selected as the research objects, and assigned into the experimental, the control and the healthy groups. The inflammation-linked factors in the three groups were compared, and multivariate Logisitic regression was applied to analyze the predictive indicators and predictive efficacy of dexamethasone treatment in children with refractory suppurative meningitis. Results: CRP, TNF-α, IL-6, PCT and IL-1β were higher in the study group vs. the control and the healthy, and in the control vs. the healthy (P < 0.05). Multivariate Logisitic regression analysis clarified the predictors of the efficacy of dexamethasone treatment in children with refractory suppurative meningitis were CRP, TNF-α, IL-6, PCT, and IL-1β, which all had good predictive performance, and among which CRP and IL-1β had better predictive performance. Conclusion: Inflammatory-linked factors have a certain predictive value for the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikola Mladenovic, Ranok Zdravkovic, Lazar Velicki, Vanja Drljevic Todic, M. Todić, S. Maletin, Aleksandra Mladenovic, Nemanja Petrovic, Bogdan R Okiljevic, Valentina Nikolić, Milan Pavlovic, Dane Krtinic, Aleksandar Nikolic, Marko Gmijovic, Aleksandar Kamenov
Uvod: Postoperativna atrijalna fibrilacije (POAF) je učestala komplikacija nakon kardiohirurških procedura. Povezana je sa produženim hospitalnim lečenjem, povećanim morbiditetom, stopom mortaliteta i finansiskim troškovima. Cilj rada je bio da se utvrdi povezanost nivoa Galektina-3 i N-terminalnog pro-B-tipa natriuretskog peptida (NT-proBNP) sa POAF nakon kardiohirurške operacije. Pacijenti i metode: Prospektivna studija obuhvata bolesnike starosti od 18-85 godina, hospitalizovanih radi elektivne operacije aorto koronarnog bajpasa (CABG) ili CABG sa zamenom aortnog zaliska. Nivoi Galektina-3 i NT-proBNP iz krvi mereni su preoperativno i postoperativnog 1. i 7. dana. Rezultati: U istraživanju je uključeno 103 bolesnika. PAOF je zabeležena kod 45 bolesnika. Prosečna starost bolesnika kod kojih je registrovan POAF bila je 68,8 godina, dok je kod ostalih bila 65,5 godina (n=0,028). Nivoi Galektina-3 i NT-proBNP nisu se razlikovali kod bolesnika koji su razvili POAF, a ni kod onih kojima nije registrovana POAF u sva tri merenja. Promene nivoa Galektin-3 prvog postoperativnog dana imaju statistički značajnu vrednost za predviđanje POAF (AUC=0.627 [0.509-0.745], p<0.05). Smanjenje nivoa Galektina-3 prvog postoperativnog dana za preko 17% dovodi do povećanog rizika od razvoj POAF . Zaključak: Preoperativne vrednosti Galektina-3 i NT-proBNP nisu povezani sa razvojem POAF nakon kardiohirurške intervencije. Jedina povezanost koju smo zabeležili je da smanjene vrednosti Galektina-3 prvog postoperativnog dana za preko 17% mogu biti prediktor za POAF.
{"title":"Značaj Galaktina-3 i N-terminalnog pro B-tipa natruretskog peptida u predviđanju atrijalne fibrilacije nakon kardiohirurške procedure","authors":"Nikola Mladenovic, Ranok Zdravkovic, Lazar Velicki, Vanja Drljevic Todic, M. Todić, S. Maletin, Aleksandra Mladenovic, Nemanja Petrovic, Bogdan R Okiljevic, Valentina Nikolić, Milan Pavlovic, Dane Krtinic, Aleksandar Nikolic, Marko Gmijovic, Aleksandar Kamenov","doi":"10.5937/jomb0-47001","DOIUrl":"https://doi.org/10.5937/jomb0-47001","url":null,"abstract":"Uvod: Postoperativna atrijalna fibrilacije (POAF) je učestala komplikacija nakon kardiohirurških procedura. Povezana je sa produženim hospitalnim lečenjem, povećanim morbiditetom, stopom mortaliteta i finansiskim troškovima. Cilj rada je bio da se utvrdi povezanost nivoa Galektina-3 i N-terminalnog pro-B-tipa natriuretskog peptida (NT-proBNP) sa POAF nakon kardiohirurške operacije. \u0000Pacijenti i metode: Prospektivna studija obuhvata bolesnike starosti od 18-85 godina, hospitalizovanih radi elektivne operacije aorto koronarnog bajpasa (CABG) ili CABG sa zamenom aortnog zaliska. Nivoi Galektina-3 i NT-proBNP iz krvi mereni su preoperativno i postoperativnog 1. i 7. dana. \u0000Rezultati: U istraživanju je uključeno 103 bolesnika. PAOF je zabeležena kod 45 bolesnika. Prosečna starost bolesnika kod kojih je registrovan POAF bila je 68,8 godina, dok je kod ostalih bila 65,5 godina (n=0,028). Nivoi Galektina-3 i NT-proBNP nisu se razlikovali kod bolesnika koji su razvili POAF, a ni kod onih kojima nije registrovana POAF u sva tri merenja. Promene nivoa Galektin-3 prvog postoperativnog dana imaju statistički značajnu vrednost za predviđanje POAF (AUC=0.627 [0.509-0.745], p<0.05). Smanjenje nivoa Galektina-3 prvog postoperativnog dana za preko 17% dovodi do povećanog rizika od razvoj POAF . \u0000Zaključak: Preoperativne vrednosti Galektina-3 i NT-proBNP nisu povezani sa razvojem POAF nakon kardiohirurške intervencije. Jedina povezanost koju smo zabeležili je da smanjene vrednosti Galektina-3 prvog postoperativnog dana za preko 17% mogu biti prediktor za POAF.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Banu Isbilen Basok, Ipek Deveci Kocakoç, Veli Iyilikci, Selena Kantarmaci, M. Fidan
Background: Laboratory professionals aim to provide a reliable laboratory service using public resources efficiently while planning a test’s procurement. This intuitive approach is ineffective, as seen in the COVID-19 pandemic, where the dramatic changes in admissions (e.g. decreased patient admissions) and the purpose of testing (e.g. D-dimer) were experienced. A model based on objective data was developed that predicts the future test consumption of coagulation tests whose consumptions were highly variable during the pandemic. Methods: Between December 2018 and July 2021, monthly consumptions of coagulation tests (PTT, aPTT, D-dimer, fibrinogen), total-, inpatient-, outpatient-, emergency-, non-emergency -admission numbers were collected. The relationship between inputs and outputs was modeled with the external input nonlinear autoregressive artificial neural network (ANN) (NARX) using MATLAB. Monthly test consumptions between January-July 2021 were used to test the models’ prediction power. Results: According to the cointegration analysis, total-, emergency-, and non-emergency admission numbers plus the number of working days per month were included in the model. When aPTT and fibrinogen consumptions were estimated, it was possible to predict the other tests. Fifty months of data were used to predict the next six months, and the NARX prediction was the more robust approach for both tests. Conclusions: The deep learning model gives better results than the intuitive approach in forecasting, even in the pandemic era, and it shows that more effective and efficient planning will be possible if ANN-supported decision mechanisms are used in forecasting tests’ consumptions in the procurement process.
{"title":"Forecasting The Consumptions of Coagulation Tests Using A Deep Learning Model","authors":"Banu Isbilen Basok, Ipek Deveci Kocakoç, Veli Iyilikci, Selena Kantarmaci, M. Fidan","doi":"10.5937/jomb0-40244","DOIUrl":"https://doi.org/10.5937/jomb0-40244","url":null,"abstract":"Background: Laboratory professionals aim to provide a reliable laboratory service using public resources efficiently while planning a test’s procurement. This intuitive approach is ineffective, as seen in the COVID-19 pandemic, where the dramatic changes in admissions (e.g. decreased patient admissions) and the purpose of testing (e.g. D-dimer) were experienced. A model based on objective data was developed that predicts the future test consumption of coagulation tests whose consumptions were highly variable during the pandemic. \u0000Methods: Between December 2018 and July 2021, monthly consumptions of coagulation tests (PTT, aPTT, D-dimer, fibrinogen), total-, inpatient-, outpatient-, emergency-, non-emergency -admission numbers were collected. The relationship between inputs and outputs was modeled with the external input nonlinear autoregressive artificial neural network (ANN) (NARX) using MATLAB. Monthly test consumptions between January-July 2021 were used to test the models’ prediction power. \u0000Results: According to the cointegration analysis, total-, emergency-, and non-emergency admission numbers plus the number of working days per month were included in the model. When aPTT and fibrinogen consumptions were estimated, it was possible to predict the other tests. Fifty months of data were used to predict the next six months, and the NARX prediction was the more robust approach for both tests. \u0000 \u0000Conclusions: The deep learning model gives better results than the intuitive approach in forecasting, even in the pandemic era, and it shows that more effective and efficient planning will be possible if ANN-supported decision mechanisms are used in forecasting tests’ consumptions in the procurement process.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138600288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To explore the variation of serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1) and squamous cell carcinoma (SCC) antigen in patients with lung cancer (LC) and their diagnostic value with endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). Methods: Selection of 150 patients with suspected lung malignant lesions as the research objects was to analyze the diagnostic value of serum tumor marker test and EBUS-TBNA joint detection for LC. Results: The AUC of joint examination to diagnose LC and identify pathological types of LC was elevated vs. alone detection of each index. Conclusions: Serum CYFRA21-1, SCC and CEA conjugating with EBUS-TBNA are provided with diagnostic value for LC and identified value for pathological types of LC.
{"title":"Analysis of variation of serum CEA, SCC, CYFRA21-1 in patients with lung cancer and their diagnostic value with EBUS-TBNA","authors":"YanJia Du, Ya Wen, JieYu Huang","doi":"10.5937/jomb0-37083","DOIUrl":"https://doi.org/10.5937/jomb0-37083","url":null,"abstract":"Objective: To explore the variation of serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1) and squamous cell carcinoma (SCC) antigen in patients with lung cancer (LC) and their diagnostic value with endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). Methods: Selection of 150 patients with suspected lung malignant lesions as the research objects was to analyze the diagnostic value of serum tumor marker test and EBUS-TBNA joint detection for LC. Results: The AUC of joint examination to diagnose LC and identify pathological types of LC was elevated vs. alone detection of each index. Conclusions: Serum CYFRA21-1, SCC and CEA conjugating with EBUS-TBNA are provided with diagnostic value for LC and identified value for pathological types of LC.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139238066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Ovarian cancer (OC) is a major gynecological malignancy with varying prognosis. The Neutrophil-to-Lymphocyte Ratio (NLR) has been proposed as a potential prognostic biomarker. This study aimed to evaluate the prognostic and clinical value of NLR in OC. Methods: A systematic review and meta-analysis were performed following PRISMA guidelines, including studies that evaluated the association between NLR and survival outcomes in OC patients. Search was performed in PubMed, Embase, Web of Science, and Cochrane Library databases. Quality assessment was done using Newcastle-Ottawa Scale (NOS). Heterogeneity was assessed, and pooled hazard ratios (HRs) were calculated using fixed or random-effects models as appropriate. Results: Twenty studies involving various ethnicities, ages, and sample sizes were included. A high NLR was found to be inversely correlated with overall survival (OS) (HR= 1.21, 95% CI 1.09-1.34, P<0.001) and progression-free survival (PFS) (HR=1.20, 95% CI 1.03-1.38, P<0.001). Stratified analyses showed a stronger association in Asian patients, studies with smaller sample sizes, younger patients, and higher NLR cutoff values. Conclusions: The meta-analysis suggests a significant inverse association between NLR and survival outcomes in OC patients, emphasizing NLR's potential as a simple, cost-effective prognostic biomarker. However, substantial heterogeneity and influence of confounding factors underscore the need for further investigation.
{"title":"The Prognostic and Clinical Value of Neutrophil-to-Lymphocyte Ratio (NLR) in Ovarian Cancer: A Systematic Review and Meta-analysis","authors":"Zihan Zhang, Jinghe Lang","doi":"10.5937/jomb0-46035","DOIUrl":"https://doi.org/10.5937/jomb0-46035","url":null,"abstract":"Background: Ovarian cancer (OC) is a major gynecological malignancy with varying prognosis. The Neutrophil-to-Lymphocyte Ratio (NLR) has been proposed as a potential prognostic biomarker. This study aimed to evaluate the prognostic and clinical value of NLR in OC. Methods: A systematic review and meta-analysis were performed following PRISMA guidelines, including studies that evaluated the association between NLR and survival outcomes in OC patients. Search was performed in PubMed, Embase, Web of Science, and Cochrane Library databases. Quality assessment was done using Newcastle-Ottawa Scale (NOS). Heterogeneity was assessed, and pooled hazard ratios (HRs) were calculated using fixed or random-effects models as appropriate. Results: Twenty studies involving various ethnicities, ages, and sample sizes were included. A high NLR was found to be inversely correlated with overall survival (OS) (HR= 1.21, 95% CI 1.09-1.34, P<0.001) and progression-free survival (PFS) (HR=1.20, 95% CI 1.03-1.38, P<0.001). Stratified analyses showed a stronger association in Asian patients, studies with smaller sample sizes, younger patients, and higher NLR cutoff values. Conclusions: The meta-analysis suggests a significant inverse association between NLR and survival outcomes in OC patients, emphasizing NLR's potential as a simple, cost-effective prognostic biomarker. However, substantial heterogeneity and influence of confounding factors underscore the need for further investigation.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139262293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The aim of this study was to explore the association between lactate/albumin ratio and the prognosis of sepsis patients.
Methods: A computerized search was performed in Pubmed, EMbase, Ovid, Medline, and Google Scholar to collate relevant studies. The results were compared using standardized mean differences (SMD)/odds ratio (OR) and 95% confidence intervals (CI). Prospective and retrospective cohort studies were both included in this study.
Results: A total of nine studies involving 3039 participants were included. Pooled analysis revealed that survivors had substantially lower lactate/albumin ratio than non-survivors (SMD=-2.02, 95% CI: -2.76 to -1.28, I2=97.4%). Further, our results also indicated that elevated lactate/albumin ratio is an independent risk factor for mortality (OR=2.16, 95% CI: 1.58 to 2.95, I2=76.2%) and multiple organ dysfunction syndrome (MODS) (OR=3.41, 95% CI: 1.78 to 6.50, I2=0.0%) in septic patients. Moreover, according to the area under curve (AUC) results, the lactate/albumin ratio also presented good discriminatory power to predict mortality (AUC=0.75, 95% CI: 0.68 to 0.84, I2=92.9%) and MODS (AUC=0.78, 95% CI: 0.68 to 0.91, I2=65.1%) in septic patients. Begg's and Egger's test suggested no publication bias in the included studies.
Conclusions: Our results highlighted that the lactate/albumin ratio is an important prognostic factor for MODS and mortality in sepsis patients, having good capabilities in identifying MODS and mortality.
{"title":"Elevated lactate/albumin ratio is associated with poor prognosis in sepsis patients: a systematic review and meta-analysis","authors":"Xian Zhao, Qin Peng, Weiwei Li, Dongmei Hu, Yue Guan, Jingwen Wang","doi":"10.5937/jomb0-42284","DOIUrl":"https://doi.org/10.5937/jomb0-42284","url":null,"abstract":"Background: The aim of this study was to explore the association between lactate/albumin ratio and the prognosis of sepsis patients.
 Methods: A computerized search was performed in Pubmed, EMbase, Ovid, Medline, and Google Scholar to collate relevant studies. The results were compared using standardized mean differences (SMD)/odds ratio (OR) and 95% confidence intervals (CI). Prospective and retrospective cohort studies were both included in this study.
 Results: A total of nine studies involving 3039 participants were included. Pooled analysis revealed that survivors had substantially lower lactate/albumin ratio than non-survivors (SMD=-2.02, 95% CI: -2.76 to -1.28, I2=97.4%). Further, our results also indicated that elevated lactate/albumin ratio is an independent risk factor for mortality (OR=2.16, 95% CI: 1.58 to 2.95, I2=76.2%) and multiple organ dysfunction syndrome (MODS) (OR=3.41, 95% CI: 1.78 to 6.50, I2=0.0%) in septic patients. Moreover, according to the area under curve (AUC) results, the lactate/albumin ratio also presented good discriminatory power to predict mortality (AUC=0.75, 95% CI: 0.68 to 0.84, I2=92.9%) and MODS (AUC=0.78, 95% CI: 0.68 to 0.91, I2=65.1%) in septic patients. Begg's and Egger's test suggested no publication bias in the included studies.
 Conclusions: Our results highlighted that the lactate/albumin ratio is an important prognostic factor for MODS and mortality in sepsis patients, having good capabilities in identifying MODS and mortality.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135036668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiang Wang, Ronghua Wang, Ying Zhou, Yao Ma, Chunyan Xiong
Background: To investigate the relationship between lactate dehydrogenase and apolipoprotein A1 levels and the condition and prognosis of patients with severe pneumonia.
Methods: We retrospectively collected 204 patients with severe pneumonia who were hospitalized from January 1, 2019 to December 1, 2021 in our hospital (respiratory intensive care unit (RICU)), and divided into survival group (160 patients) and death group (44 patients) according to their hospitalization outcome. The relationship between lactate dehydrogenase and apolipoprotein A1 levels and general information, disease, and treatment needs of patients with severe pneumonia was analyzed, and lactate dehydrogenase, apolipoprotein A1, neutrophil-to-lymphocyte ratio, hematocrit, C-reactive protein, calcitoninogen, D-dimer, Acute Physiology and Chronic Health Status Rating System II, and Pneumonia Severity Index scores were compared between the survival and death groups. The value of these indicators in determining the prognosis of patients was analyzed using subject operating characteristic (ROC) curves. Logistic regression was used to analyze the risk factors for death from severe pneumonia.
Results: The differences were statistically significant (P<0.05) when comparing age and pneumonia typing between the two groups. There was no statistically significant difference between the two groups in terms of gender and total length of stay (P >0.05). There was no statistically significant difference in LDH and ApoA1 levels between male patients and female patients (P>0.05). The differences in LDH and ApoA1 levels were statistically significant (P<0.05) when comparing patients with severe pneumonia at different ages. The differences in LDH and ApoA1 levels between SCAP and SHAP patients were not statistically significant (P>0.05). LDH and ApoA1 levels were higher in patients with severe pneumonia with acute exacerbation of slow-onset lung or MODS during hospitalization than in patients with severe pneumonia without acute exacerbation of slow-onset lung or MODS, with statistically significant differences (P<0.05). The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different PSI grades or APACHE II scores. The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different ICU length of stay. There was no statistically significant difference in LDH and ApoA1 levels when comparing patients with severe pneumonia who required tracheal intubation or sedation and analgesia during hospitalization (P>0.05). LDH and ApoA1 levels in patients with severe pneumonia with different duration of mechanical ventilation were compared with statistically significant differences (P<0.05). LDH and ApoA1 levels in the death group were 105.08 (75.22 ~140.0), which was significantly higher than 86.66 (62.66 ~ 106.14) in the survival group, with statisti
{"title":"The relationship between lactate dehydrogenase and apolipoprotein A1 levels in patients with severe pneumonia","authors":"Jiang Wang, Ronghua Wang, Ying Zhou, Yao Ma, Chunyan Xiong","doi":"10.5937/jomb0-45782","DOIUrl":"https://doi.org/10.5937/jomb0-45782","url":null,"abstract":"Background: To investigate the relationship between lactate dehydrogenase and apolipoprotein A1 levels and the condition and prognosis of patients with severe pneumonia.
 Methods: We retrospectively collected 204 patients with severe pneumonia who were hospitalized from January 1, 2019 to December 1, 2021 in our hospital (respiratory intensive care unit (RICU)), and divided into survival group (160 patients) and death group (44 patients) according to their hospitalization outcome. The relationship between lactate dehydrogenase and apolipoprotein A1 levels and general information, disease, and treatment needs of patients with severe pneumonia was analyzed, and lactate dehydrogenase, apolipoprotein A1, neutrophil-to-lymphocyte ratio, hematocrit, C-reactive protein, calcitoninogen, D-dimer, Acute Physiology and Chronic Health Status Rating System II, and Pneumonia Severity Index scores were compared between the survival and death groups. The value of these indicators in determining the prognosis of patients was analyzed using subject operating characteristic (ROC) curves. Logistic regression was used to analyze the risk factors for death from severe pneumonia.
 Results: The differences were statistically significant (P<0.05) when comparing age and pneumonia typing between the two groups. There was no statistically significant difference between the two groups in terms of gender and total length of stay (P >0.05). There was no statistically significant difference in LDH and ApoA1 levels between male patients and female patients (P>0.05). The differences in LDH and ApoA1 levels were statistically significant (P<0.05) when comparing patients with severe pneumonia at different ages. The differences in LDH and ApoA1 levels between SCAP and SHAP patients were not statistically significant (P>0.05). LDH and ApoA1 levels were higher in patients with severe pneumonia with acute exacerbation of slow-onset lung or MODS during hospitalization than in patients with severe pneumonia without acute exacerbation of slow-onset lung or MODS, with statistically significant differences (P<0.05). The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different PSI grades or APACHE II scores. The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different ICU length of stay. There was no statistically significant difference in LDH and ApoA1 levels when comparing patients with severe pneumonia who required tracheal intubation or sedation and analgesia during hospitalization (P>0.05). LDH and ApoA1 levels in patients with severe pneumonia with different duration of mechanical ventilation were compared with statistically significant differences (P<0.05). LDH and ApoA1 levels in the death group were 105.08 (75.22 ~140.0), which was significantly higher than 86.66 (62.66 ~ 106.14) in the survival group, with statisti","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135286390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilaria Talli, Andrea Padoan, Stefania Moz, Filippo Navaglia, Mario Plebani, Daniela Basso
Background: Molecular testing is considered the gold standard for the detection of SARS-CoV-2. This study aimed to compare the performance of the P742H SARS-CoV-2 Nucleic Acid Multiplex Detection Kit in salivary samples, with respect to the 732HF Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit and the TaqPath COVID-19 CEIVD RT-PCR Kit, used at University-Hospital of Padova, Italy.
Methods: One hundred twenty-four salivary samples selfcollected by healthcare workers (HCW) during the screening program at University-Hospital of Padova, Italy, from Oct to Nov 2022, were included in the study. RNA extraction was performed by Viral DNA and RNA Extraction Kit (Technogenetics, Lodi, Italy) and amplification by P742H and 732HF (Technogenetics, Lodi, Italy). RNA was extracted using MagNa Pure 96 DNA and Viral NA Small Volume Kit (Roche, Switzerland) for TaqPath analysis (Thermo Fisher Scientific, USA).
背景：分子检测被认为是检测SARS-CoV-2的黄金标准。本研究旨在比较 P742H SARS-CoV-2 核酸多重检测试剂盒与意大利帕多瓦大学医院使用的 732HF 新型冠状病毒（2019-nCoV）核酸检测试剂盒和 TaqPath COVID-19 CEIVD RT-PCR 试剂盒在唾液样本中的性能：研究纳入了124份医护人员（HCW）于2022年10月至11月在意大利帕多瓦大学医院筛查项目中自行采集的唾液样本。使用病毒 DNA 和 RNA 提取试剂盒（Technogenetics，意大利洛迪）提取 RNA，并使用 P742H 和 732HF （Technogenetics，意大利洛迪）进行扩增。使用 MagNa Pure 96 DNA 和病毒 NA 小容量试剂盒（瑞士罗氏）提取 RNA，用于 TaqPath 分析（美国赛默飞世尔科技公司）。
{"title":"Evaluation of a new molecular test for the detection of SARS-CoV-2 nucleic acid in salivary samples.","authors":"Ilaria Talli, Andrea Padoan, Stefania Moz, Filippo Navaglia, Mario Plebani, Daniela Basso","doi":"10.5937/jomb0-43822","DOIUrl":"10.5937/jomb0-43822","url":null,"abstract":"<p><strong>Background: </strong>Molecular testing is considered the gold standard for the detection of SARS-CoV-2. This study aimed to compare the performance of the P742H SARS-CoV-2 Nucleic Acid Multiplex Detection Kit in salivary samples, with respect to the 732HF Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit and the TaqPath COVID-19 CEIVD RT-PCR Kit, used at University-Hospital of Padova, Italy.</p><p><strong>Methods: </strong>One hundred twenty-four salivary samples selfcollected by healthcare workers (HCW) during the screening program at University-Hospital of Padova, Italy, from Oct to Nov 2022, were included in the study. RNA extraction was performed by Viral DNA and RNA Extraction Kit (Technogenetics, Lodi, Italy) and amplification by P742H and 732HF (Technogenetics, Lodi, Italy). RNA was extracted using MagNa Pure 96 DNA and Viral NA Small Volume Kit (Roche, Switzerland) for TaqPath analysis (Thermo Fisher Scientific, USA).</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76305752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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