Zlatko Pravdić, Nada Suvajdžić-Vuković, Marijana Virijević, Mirjana Mitrović, Nikola Pantić, Nikica Sabljić, Đorđe Pavlović, Irena Marjanović, Zoran Bukumirić, Ana Vidović, Ljubomir Jaković, Sonja Pavlović, Vladimir Gašić
Background: Cytarabine-anthracycline-based induction chemotherapy remains the standard of care for remission induction among patients with newly diagnosed acute myeloid leukaemia (AML). There are remarkable differences in therapy response among AML patients. This fact could be partly explained by the patients' genetic variability related to the metabolic paths of cytarabine and anthracyclines. This study aims to evaluate the effect of variants in pharmacogenes SLC29A1, DCK, ABCB1, GSTM1, and GSTT1, as well as laboratory and AML-related parameters on clinical outcomes in adult AML patients.
Methods: A total of 100 AML patients were included in the study. Pharmacogenetic variants SLC29A1 rs9394992, DCK rs12648166, ABCB1 rs2032582, and GSTM1 and GSTT1 gene deletions were detected by methodology based on PCR, fragment analysis and direct sequencing. The methods of descriptive and analytic statistics were used. Survival analysis was done using the Kaplan-Meier method using the Log-Rank test.
Results: This is the first study of adult AML pharmacogenetics in the Serbian population. Clinical outcomes in our cohort of AML patients were not impacted by analysed variants in SLC29A1, DCK, ABCB1 and GSTT1, and GSTM1 genes, independently or in combinations. Achievement of complete remission was identified as an independent prognostic indicator of clinical outcome.
Conclusions: The population-specific genomic profile has to be considered in pharmacogenetics. Since the data on AML pharmacogenetics in European populations is limited, our results contribute to knowledge in this field and strongly indicate that a high-throughput approach must be applied to find particular pharmacogenetic markers of AML in the European population.
背景：以阿糖胞苷-蒽环类为基础的诱导化疗仍是新诊断急性髓性白血病（AML）患者缓解诱导治疗的标准疗法。急性髓性白血病患者的治疗反应存在明显差异。这一事实的部分原因可能是患者与阿糖胞苷和蒽环类药物代谢途径有关的基因变异。本研究旨在评估药物基因 SLC29A1、DCK、ABCB1、GSTM1 和 GSTT1 的变异以及实验室和 AML 相关参数对成年 AML 患者临床结果的影响：研究共纳入了 100 名急性髓细胞性白血病患者。通过PCR、片段分析和直接测序等方法检测药物基因变异SLC29A1 rs9394992、DCK rs12648166、ABCB1 rs2032582以及GSTM1和GSTT1基因缺失。采用了描述性和分析性统计方法。采用 Kaplan-Meier 法和 Log-Rank 检验法进行生存率分析：这是首次在塞尔维亚人群中开展成人急性髓细胞白血病药物遗传学研究。SLC29A1、DCK、ABCB1和GSTT1以及GSTM1基因中的变异，无论是单独还是组合出现，都不会影响我们队列中急性髓细胞性白血病患者的临床预后。完全缓解被认为是临床结果的独立预后指标：结论：药物遗传学必须考虑特定人群的基因组特征。由于欧洲人群中有关急性髓细胞性白血病药物遗传学的数据有限，我们的研究结果为这一领域的知识做出了贡献，并有力地表明必须采用高通量方法来寻找欧洲人群中急性髓细胞性白血病的特定药物遗传学标志物。
{"title":"Can pharmacogenetics impact the therapeutic effect of cytarabine and anthracyclines in adult acute myeloid leukaemia patients?: A Serbian experience.","authors":"Zlatko Pravdić, Nada Suvajdžić-Vuković, Marijana Virijević, Mirjana Mitrović, Nikola Pantić, Nikica Sabljić, Đorđe Pavlović, Irena Marjanović, Zoran Bukumirić, Ana Vidović, Ljubomir Jaković, Sonja Pavlović, Vladimir Gašić","doi":"10.5937/jomb0-47459","DOIUrl":"10.5937/jomb0-47459","url":null,"abstract":"<p><strong>Background: </strong>Cytarabine-anthracycline-based induction chemotherapy remains the standard of care for remission induction among patients with newly diagnosed acute myeloid leukaemia (AML). There are remarkable differences in therapy response among AML patients. This fact could be partly explained by the patients' genetic variability related to the metabolic paths of cytarabine and anthracyclines. This study aims to evaluate the effect of variants in pharmacogenes SLC29A1, DCK, ABCB1, GSTM1, and GSTT1, as well as laboratory and AML-related parameters on clinical outcomes in adult AML patients.</p><p><strong>Methods: </strong>A total of 100 AML patients were included in the study. Pharmacogenetic variants SLC29A1 rs9394992, DCK rs12648166, ABCB1 rs2032582, and GSTM1 and GSTT1 gene deletions were detected by methodology based on PCR, fragment analysis and direct sequencing. The methods of descriptive and analytic statistics were used. Survival analysis was done using the Kaplan-Meier method using the Log-Rank test.</p><p><strong>Results: </strong>This is the first study of adult AML pharmacogenetics in the Serbian population. Clinical outcomes in our cohort of AML patients were not impacted by analysed variants in SLC29A1, DCK, ABCB1 and GSTT1, and GSTM1 genes, independently or in combinations. Achievement of complete remission was identified as an independent prognostic indicator of clinical outcome.</p><p><strong>Conclusions: </strong>The population-specific genomic profile has to be considered in pharmacogenetics. Since the data on AML pharmacogenetics in European populations is limited, our results contribute to knowledge in this field and strongly indicate that a high-throughput approach must be applied to find particular pharmacogenetic markers of AML in the European population.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"43 4","pages":"545-555"},"PeriodicalIF":2.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To evaluate the prognostic value of blood urea nitrogen/creatinine ratio (BUN/SCr) and cystatin C (Cys C) in patients with renal cell carcinoma (RCC) after radical nephrectomy.
Methods: The study analysed 348 patients with RCC who underwent radical nephrectomy. The optimal cut-off was obtained based on the ROC of specific survival outcomes and the maximum Youden index. The patients were divided into four groups: Group 1 (low BUN/SCr-low Cys C), Group 2 (low BUN/SCr-high Cys C), Group 3 (high BUN/SCr-low Cys C), and Group 4 (high BUN/SCr-high Cys C). The primary endpoint was cancer-specific survival (CSS), and the secondary endpoint was disease-free survival (DFS).
{"title":"Prognostic values of blood urea nitrogen/creatinine and cystatin C in patients with radical nephrectomy for renal cell carcinoma.","authors":"SiCheng Wang, HaoLong Chen, Feng Chao, Jia Bin","doi":"10.5937/jomb0-45664","DOIUrl":"10.5937/jomb0-45664","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the prognostic value of blood urea nitrogen/creatinine ratio (BUN/SCr) and cystatin C (Cys C) in patients with renal cell carcinoma (RCC) after radical nephrectomy.</p><p><strong>Methods: </strong>The study analysed 348 patients with RCC who underwent radical nephrectomy. The optimal cut-off was obtained based on the ROC of specific survival outcomes and the maximum Youden index. The patients were divided into four groups: Group 1 (low BUN/SCr-low Cys C), Group 2 (low BUN/SCr-high Cys C), Group 3 (high BUN/SCr-low Cys C), and Group 4 (high BUN/SCr-high Cys C). The primary endpoint was cancer-specific survival (CSS), and the secondary endpoint was disease-free survival (DFS).</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"43 4","pages":"436-444"},"PeriodicalIF":2.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This study aimed to identify pathogens and factors that predict the outcome of severe COVID-19 by utilizing metagenomic next-generation sequencing (mNGS) technology.
Methods: We retrospectively analyzed data from 56 severe COVID-19 patients admitted to our hospital between December 2022 and March 2023. We analyzed the pathogen types and strains detected through mNGS and conventional microbiological testing and collected general patient information.
Results: In this study, 42 pathogens were detected using mNGS and conventional microbiological testing. mNGS had a significantly higher detection rate of 90.48% compared to 71.43% for conventional testing (P=0.026). A total of 196 strains were detected using both methods, with a significantly higher detection rate of 70.92% for mNGS compared to 49.49% for conventional testing (P=0.000). The 56 patients were divided into a survival group (33 cases) and a death group (23 cases) based on clinical outcomes. The survival group had significantly lower age, number of pathogens detected by mNGS, number of pathogens detected by conventional testing, APACHE-II score, SOFA score, high-sensitivity troponin, creatine kinase-MB subtype, and lactate dehydrogenase compared to the death group (P<0.05). Multivariate logistic regression analysis showed that these factors were risk factors for mortality in severe COVID-19 patients (P<0.05). In contrast, ROC curve analysis revealed that these factors had diagnostic values for mortality, with AUC values ranging from 0.657 to 0.963. The combined diagnosis of these indicators had an AUC of 0.924.
Conclusions: The use of mNGS technology can significantly enhance the detection of pathogens in severe cases of COVID-19 and also has a solid ability to predict clinical outcomes.
{"title":"Exploring the pathogen diagnosis and prognostic factors of severe COVID-19 using metagenomic next-generation sequencing: A retrospective study.","authors":"Weizhong Zeng, Yanchao Liang, Xiaoyuan He, Fangwei Chen, Jiali Xiong, Zhenhua Wen, Liang Tang, Xun Chen, Juan Zhang","doi":"10.5937/jomb0-49102","DOIUrl":"10.5937/jomb0-49102","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to identify pathogens and factors that predict the outcome of severe COVID-19 by utilizing metagenomic next-generation sequencing (mNGS) technology.</p><p><strong>Methods: </strong>We retrospectively analyzed data from 56 severe COVID-19 patients admitted to our hospital between December 2022 and March 2023. We analyzed the pathogen types and strains detected through mNGS and conventional microbiological testing and collected general patient information.</p><p><strong>Results: </strong>In this study, 42 pathogens were detected using mNGS and conventional microbiological testing. mNGS had a significantly higher detection rate of 90.48% compared to 71.43% for conventional testing (P=0.026). A total of 196 strains were detected using both methods, with a significantly higher detection rate of 70.92% for mNGS compared to 49.49% for conventional testing (P=0.000). The 56 patients were divided into a survival group (33 cases) and a death group (23 cases) based on clinical outcomes. The survival group had significantly lower age, number of pathogens detected by mNGS, number of pathogens detected by conventional testing, APACHE-II score, SOFA score, high-sensitivity troponin, creatine kinase-MB subtype, and lactate dehydrogenase compared to the death group (P<0.05). Multivariate logistic regression analysis showed that these factors were risk factors for mortality in severe COVID-19 patients (P<0.05). In contrast, ROC curve analysis revealed that these factors had diagnostic values for mortality, with AUC values ranging from 0.657 to 0.963. The combined diagnosis of these indicators had an AUC of 0.924.</p><p><strong>Conclusions: </strong>The use of mNGS technology can significantly enhance the detection of pathogens in severe cases of COVID-19 and also has a solid ability to predict clinical outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"43 4","pages":"528-536"},"PeriodicalIF":2.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Gojković, S. Vladimirov, T. Antonić, N. Bogavac-Stanojević, Katarina Novovic, V. Spasojević-Kalimanovska, B. Filipić
Background: The pre-analytical (PA) phase is the most vulnerable phase of laboratory testing procedure, with critical procedures-collection, handling, sample transport, and time and temperature of sample storage. The aim of this study was to examine if different anticoagulants, storage conditions, and freeze-thaw cycles (FTCs) influence the concentrations of basic biochemical parameters. In parallel, the presence and the effect of sample microbiological contamination during routine laboratory work were examined. Methods: Two plasma pools (EDTA, and sodium-fluoride/potassium oxalate plasma (NaF)) were stored at +4C˚/-20˚C. Total cholesterol (TC), glucose, triglycerides (TG), urea, total protein (TP), and albumin concentrations were measured using Ilab 300+. Sample microbiological contamination was determined by 16S rRNA sequence analysis. The experiment encompassed a 5 day-period: Day 1–fresh sample, Day 2–1st FTC, Day 3–2nd FTC, Day 4–3rd FTC, Day 5–4th FTC. The appearance of bacteria in two consecutive samples was the experiment's endpoint. Results: During 4 FTCs there were no changes in plasma urea concentrations. Glucose was stable in EDTA+4˚C and NaF- 20˚C until the 3rd FTC (P=0.008, P=0.042, respectively). Changes in protein concentrations followed the zig-zag pattern. TG concentrations changed significantly in the EDTA-20˚C sample after 1st and 4th FTCs (P=0.022, P=0.010, respectively). In NaF samples no contamination was observed during 4 FTCs. Conclusions: Urea and glucose concentrations were robust. Changes in lipid and protein concentrations after FTCs follow complex patterns. Bacterial growth was not observed in NaF plasma samples. This can promote NaF use in analytical procedures in which microbiological contamination affects the quality of analysis.
{"title":"The influence of various sample storage conditions and sample microbial contamination on concentrations of routine biochemical parameters","authors":"T. Gojković, S. Vladimirov, T. Antonić, N. Bogavac-Stanojević, Katarina Novovic, V. Spasojević-Kalimanovska, B. Filipić","doi":"10.5937/jomb0-40360","DOIUrl":"https://doi.org/10.5937/jomb0-40360","url":null,"abstract":"Background: The pre-analytical (PA) phase is the most vulnerable phase of laboratory testing procedure, with critical procedures-collection, handling, sample transport, and time and temperature of sample storage. The aim of this study was to examine if different anticoagulants, storage conditions, and freeze-thaw cycles (FTCs) influence the concentrations of basic biochemical parameters. In parallel, the presence and the effect of sample microbiological contamination during routine laboratory work were examined. Methods: Two plasma pools (EDTA, and sodium-fluoride/potassium oxalate plasma (NaF)) were stored at +4C˚/-20˚C. Total cholesterol (TC), glucose, triglycerides (TG), urea, total protein (TP), and albumin concentrations were measured using Ilab 300+. Sample microbiological contamination was determined by 16S rRNA sequence analysis. The experiment encompassed a 5 day-period: Day 1–fresh sample, Day 2–1st FTC, Day 3–2nd FTC, Day 4–3rd FTC, Day 5–4th FTC. The appearance of bacteria in two consecutive samples was the experiment's endpoint. Results: During 4 FTCs there were no changes in plasma urea concentrations. Glucose was stable in EDTA+4˚C and NaF- 20˚C until the 3rd FTC (P=0.008, P=0.042, respectively). Changes in protein concentrations followed the zig-zag pattern. TG concentrations changed significantly in the EDTA-20˚C sample after 1st and 4th FTCs (P=0.022, P=0.010, respectively). In NaF samples no contamination was observed during 4 FTCs. Conclusions: Urea and glucose concentrations were robust. Changes in lipid and protein concentrations after FTCs follow complex patterns. Bacterial growth was not observed in NaF plasma samples. This can promote NaF use in analytical procedures in which microbiological contamination affects the quality of analysis.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"9 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qin Hu, Yu Mao, Haomiao Lan, Yi Wei, Yuehua Chen, Qiang Ye, Hongying Che
Background: The purpose of this study was to investigate the potential of plasma cfDNA methylation patterns in reflecting tumor methylation changes, focusing on three candidate sites, cg02469161, cg11528914, and cg20131654. These sites were selected for verification, with a particular emphasis on their association with breast cancer. Methods: We conducted a comprehensive analysis of 850k whole-methylation sequencing data to identify potential markers for breast cancer detection. Subsequently, we examined the methylation status of genes RANBP3, LCP2, and GRAP2, where the aforementioned sites are located, using cancer and cancer-adjacent tissues from 17 breast cancer patients. We also assessed the methylation patterns in different molecular subtypes and pathological grades of breast cancer. Additionally, we compared the methylation levels of these genes in plasma cfDNA to their performance in tissues. Results: Our analysis revealed that RANBP3, LCP2, and GRAP2 genes exhibited significant methylation differences between cancer and cancer-adjacent tissues. In breast cancer, these genes displayed diagnostic efficiencies of 91.0%, 90.6%, and 92.2%, respectively. Notably, RANBP3 showed a tendency towards lower methylation in HR+ breast cancer, and LCP2 methylation was correlated with tumor malignancy. Importantly, the methylation levels of these three genes in plasma cfDNA closely mirrored their tissue counterparts, with diagnostic efficiencies of 83.3%, 83.9%, and 77.6% for RANBP3, LCP2, and GRAP2, respectively. Conclusions: Our findings suggest that RANBP3, LCP2, and GRAP2 genes, located at the identified methylation sites, can serve as valuable blood molecular markers for the auxiliary diagnosis of breast cancer. This research provides a foundation for further exploration of gene methylation pattern changes in cfDNA for the detection of breast cancer and other cancer types.
{"title":"Value of altered methylation patterns of genes RANBP3, LCP2 and GRAP2 in cfDNA in breast cancer diagnosis","authors":"Qin Hu, Yu Mao, Haomiao Lan, Yi Wei, Yuehua Chen, Qiang Ye, Hongying Che","doi":"10.5937/jomb0-47507","DOIUrl":"https://doi.org/10.5937/jomb0-47507","url":null,"abstract":"Background: The purpose of this study was to investigate the potential of plasma cfDNA methylation patterns in reflecting tumor methylation changes, focusing on three candidate sites, cg02469161, cg11528914, and cg20131654. These sites were selected for verification, with a particular emphasis on their association with breast cancer. \u0000Methods: We conducted a comprehensive analysis of 850k whole-methylation sequencing data to identify potential markers for breast cancer detection. Subsequently, we examined the methylation status of genes RANBP3, LCP2, and GRAP2, where the aforementioned sites are located, using cancer and cancer-adjacent tissues from 17 breast cancer patients. We also assessed the methylation patterns in different molecular subtypes and pathological grades of breast cancer. Additionally, we compared the methylation levels of these genes in plasma cfDNA to their performance in tissues. \u0000Results: Our analysis revealed that RANBP3, LCP2, and GRAP2 genes exhibited significant methylation differences between cancer and cancer-adjacent tissues. In breast cancer, these genes displayed diagnostic efficiencies of 91.0%, 90.6%, and 92.2%, respectively. Notably, RANBP3 showed a tendency towards lower methylation in HR+ breast cancer, and LCP2 methylation was correlated with tumor malignancy. Importantly, the methylation levels of these three genes in plasma cfDNA closely mirrored their tissue counterparts, with diagnostic efficiencies of 83.3%, 83.9%, and 77.6% for RANBP3, LCP2, and GRAP2, respectively. \u0000Conclusions: Our findings suggest that RANBP3, LCP2, and GRAP2 genes, located at the identified methylation sites, can serve as valuable blood molecular markers for the auxiliary diagnosis of breast cancer. This research provides a foundation for further exploration of gene methylation pattern changes in cfDNA for the detection of breast cancer and other cancer types.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"112 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138965065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective:It aims to explore the effect of target task oriented phase training on fibrinogen (Fbg), angiopoietin (Ang-1), vascular endothelial growth factor (VEGF), serum brain derived neurotrophic factor (BDNF) and quality of life in postoperative patients with brain trauma. Methods:142 patients with brain trauma who were operated in neurosurgery of our hospital from March 2020 to March 2023 are chosen and separated into two groups by random number table. The control group (n=71) receive routine postoperative training, and the experimental group (n=71) receive target task oriented training based on the control group, and the serum cell levels of nursing for 3, 7 and 14 days are compared. Improvement of limb function and quality of life after 2, 4, and 6 weeks of nursing care is observed. Results: Before nursing, the comparison in serum factor levels, limb function scores, and quality of life scores between the two groups was with P>0.05; After 3, 7, and 14 days of nursing, the Fbg of the control group was higher than that of the experimental group; The Ang-1, VEGF, and BDNF levels in the experimental group were higher than those in the control group (P<0.05); After 2, 4, and 6 weeks of nursing care, the FMA scores of the upper and lower limbs in the control group were lower than those in the experimental group, with P<0.05; The scores in the physiological, environmental, psychological, and social fields of thecontrol group were lower than those of the experimental group, with P<0.05. Conclusion: The application of target task oriented phase training in patients with brain trauma after surgery can help promote the serum levels of Fbg, Ang-1, VEGF, and BDNF, improve limb function, and enhance quality of life.
{"title":"the The Improvement of Fbg, Ang-1, VEGF, BDNF in Postoperative Patients with Brain Trauma Through Target Task Oriented Phrase Training","authors":"Bo Liu","doi":"10.5937/jomb0-45490","DOIUrl":"https://doi.org/10.5937/jomb0-45490","url":null,"abstract":"Objective:It aims to explore the effect of target task oriented phase training on fibrinogen (Fbg), angiopoietin (Ang-1), vascular endothelial growth factor (VEGF), serum brain derived neurotrophic factor (BDNF) and quality of life in postoperative patients with brain trauma. Methods:142 patients with brain trauma who were operated in neurosurgery of our hospital from March 2020 to March 2023 are chosen and separated into two groups by random number table. The control group (n=71) receive routine postoperative training, and the experimental group (n=71) receive target task oriented training based on the control group, and the serum cell levels of nursing for 3, 7 and 14 days are compared. Improvement of limb function and quality of life after 2, 4, and 6 weeks of nursing care is observed. Results: Before nursing, the comparison in serum factor levels, limb function scores, and quality of life scores between the two groups was with P>0.05; After 3, 7, and 14 days of nursing, the Fbg of the control group was higher than that of the experimental group; The Ang-1, VEGF, and BDNF levels in the experimental group were higher than those in the control group (P<0.05); After 2, 4, and 6 weeks of nursing care, the FMA scores of the upper and lower limbs in the control group were lower than those in the experimental group, with P<0.05; The scores in the physiological, environmental, psychological, and social fields of thecontrol group were lower than those of the experimental group, with P<0.05. Conclusion: The application of target task oriented phase training in patients with brain trauma after surgery can help promote the serum levels of Fbg, Ang-1, VEGF, and BDNF, improve limb function, and enhance quality of life.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The aim of this study was to figure out the predictive value of inflammatory-linked factors on the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children. Methods: Using regression analysis method, 38 children with refractory purulent meningitis, 40 children with purulent meningitis, and 40 healthy children who came to Qingtian County People's Hospital of Lishui City for physical examination during the same period were selected as the research objects, and assigned into the experimental, the control and the healthy groups. The inflammation-linked factors in the three groups were compared, and multivariate Logisitic regression was applied to analyze the predictive indicators and predictive efficacy of dexamethasone treatment in children with refractory suppurative meningitis. Results: CRP, TNF-α, IL-6, PCT and IL-1β were higher in the study group vs. the control and the healthy, and in the control vs. the healthy (P < 0.05). Multivariate Logisitic regression analysis clarified the predictors of the efficacy of dexamethasone treatment in children with refractory suppurative meningitis were CRP, TNF-α, IL-6, PCT, and IL-1β, which all had good predictive performance, and among which CRP and IL-1β had better predictive performance. Conclusion: Inflammatory-linked factors have a certain predictive value for the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children.
{"title":"Predictive value of inflammatory-related factors on the efficacy of adjuvant dexamethasone in the treatment of children with refractory suppurative meningitis","authors":"Kang Xu, XiaoMei Zhong, QingJun Niu","doi":"10.5937/jomb0-37618","DOIUrl":"https://doi.org/10.5937/jomb0-37618","url":null,"abstract":"Purpose: The aim of this study was to figure out the predictive value of inflammatory-linked factors on the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children. Methods: Using regression analysis method, 38 children with refractory purulent meningitis, 40 children with purulent meningitis, and 40 healthy children who came to Qingtian County People's Hospital of Lishui City for physical examination during the same period were selected as the research objects, and assigned into the experimental, the control and the healthy groups. The inflammation-linked factors in the three groups were compared, and multivariate Logisitic regression was applied to analyze the predictive indicators and predictive efficacy of dexamethasone treatment in children with refractory suppurative meningitis. Results: CRP, TNF-α, IL-6, PCT and IL-1β were higher in the study group vs. the control and the healthy, and in the control vs. the healthy (P < 0.05). Multivariate Logisitic regression analysis clarified the predictors of the efficacy of dexamethasone treatment in children with refractory suppurative meningitis were CRP, TNF-α, IL-6, PCT, and IL-1β, which all had good predictive performance, and among which CRP and IL-1β had better predictive performance. Conclusion: Inflammatory-linked factors have a certain predictive value for the efficacy of dexamethasone adjuvant therapy for refractory suppurative meningitis in children.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"183 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikola Mladenovic, Ranok Zdravkovic, Lazar Velicki, Vanja Drljevic Todic, M. Todić, S. Maletin, Aleksandra Mladenovic, Nemanja Petrovic, Bogdan R Okiljevic, Valentina Nikolić, Milan Pavlovic, Dane Krtinic, Aleksandar Nikolic, Marko Gmijovic, Aleksandar Kamenov
Uvod: Postoperativna atrijalna fibrilacije (POAF) je učestala komplikacija nakon kardiohirurških procedura. Povezana je sa produženim hospitalnim lečenjem, povećanim morbiditetom, stopom mortaliteta i finansiskim troškovima. Cilj rada je bio da se utvrdi povezanost nivoa Galektina-3 i N-terminalnog pro-B-tipa natriuretskog peptida (NT-proBNP) sa POAF nakon kardiohirurške operacije. Pacijenti i metode: Prospektivna studija obuhvata bolesnike starosti od 18-85 godina, hospitalizovanih radi elektivne operacije aorto koronarnog bajpasa (CABG) ili CABG sa zamenom aortnog zaliska. Nivoi Galektina-3 i NT-proBNP iz krvi mereni su preoperativno i postoperativnog 1. i 7. dana. Rezultati: U istraživanju je uključeno 103 bolesnika. PAOF je zabeležena kod 45 bolesnika. Prosečna starost bolesnika kod kojih je registrovan POAF bila je 68,8 godina, dok je kod ostalih bila 65,5 godina (n=0,028). Nivoi Galektina-3 i NT-proBNP nisu se razlikovali kod bolesnika koji su razvili POAF, a ni kod onih kojima nije registrovana POAF u sva tri merenja. Promene nivoa Galektin-3 prvog postoperativnog dana imaju statistički značajnu vrednost za predviđanje POAF (AUC=0.627 [0.509-0.745], p<0.05). Smanjenje nivoa Galektina-3 prvog postoperativnog dana za preko 17% dovodi do povećanog rizika od razvoj POAF . Zaključak: Preoperativne vrednosti Galektina-3 i NT-proBNP nisu povezani sa razvojem POAF nakon kardiohirurške intervencije. Jedina povezanost koju smo zabeležili je da smanjene vrednosti Galektina-3 prvog postoperativnog dana za preko 17% mogu biti prediktor za POAF.
{"title":"Značaj Galaktina-3 i N-terminalnog pro B-tipa natruretskog peptida u predviđanju atrijalne fibrilacije nakon kardiohirurške procedure","authors":"Nikola Mladenovic, Ranok Zdravkovic, Lazar Velicki, Vanja Drljevic Todic, M. Todić, S. Maletin, Aleksandra Mladenovic, Nemanja Petrovic, Bogdan R Okiljevic, Valentina Nikolić, Milan Pavlovic, Dane Krtinic, Aleksandar Nikolic, Marko Gmijovic, Aleksandar Kamenov","doi":"10.5937/jomb0-47001","DOIUrl":"https://doi.org/10.5937/jomb0-47001","url":null,"abstract":"Uvod: Postoperativna atrijalna fibrilacije (POAF) je učestala komplikacija nakon kardiohirurških procedura. Povezana je sa produženim hospitalnim lečenjem, povećanim morbiditetom, stopom mortaliteta i finansiskim troškovima. Cilj rada je bio da se utvrdi povezanost nivoa Galektina-3 i N-terminalnog pro-B-tipa natriuretskog peptida (NT-proBNP) sa POAF nakon kardiohirurške operacije. \u0000Pacijenti i metode: Prospektivna studija obuhvata bolesnike starosti od 18-85 godina, hospitalizovanih radi elektivne operacije aorto koronarnog bajpasa (CABG) ili CABG sa zamenom aortnog zaliska. Nivoi Galektina-3 i NT-proBNP iz krvi mereni su preoperativno i postoperativnog 1. i 7. dana. \u0000Rezultati: U istraživanju je uključeno 103 bolesnika. PAOF je zabeležena kod 45 bolesnika. Prosečna starost bolesnika kod kojih je registrovan POAF bila je 68,8 godina, dok je kod ostalih bila 65,5 godina (n=0,028). Nivoi Galektina-3 i NT-proBNP nisu se razlikovali kod bolesnika koji su razvili POAF, a ni kod onih kojima nije registrovana POAF u sva tri merenja. Promene nivoa Galektin-3 prvog postoperativnog dana imaju statistički značajnu vrednost za predviđanje POAF (AUC=0.627 [0.509-0.745], p<0.05). Smanjenje nivoa Galektina-3 prvog postoperativnog dana za preko 17% dovodi do povećanog rizika od razvoj POAF . \u0000Zaključak: Preoperativne vrednosti Galektina-3 i NT-proBNP nisu povezani sa razvojem POAF nakon kardiohirurške intervencije. Jedina povezanost koju smo zabeležili je da smanjene vrednosti Galektina-3 prvog postoperativnog dana za preko 17% mogu biti prediktor za POAF.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"36 22","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Banu Isbilen Basok, Ipek Deveci Kocakoç, Veli Iyilikci, Selena Kantarmaci, M. Fidan
Background: Laboratory professionals aim to provide a reliable laboratory service using public resources efficiently while planning a test’s procurement. This intuitive approach is ineffective, as seen in the COVID-19 pandemic, where the dramatic changes in admissions (e.g. decreased patient admissions) and the purpose of testing (e.g. D-dimer) were experienced. A model based on objective data was developed that predicts the future test consumption of coagulation tests whose consumptions were highly variable during the pandemic. Methods: Between December 2018 and July 2021, monthly consumptions of coagulation tests (PTT, aPTT, D-dimer, fibrinogen), total-, inpatient-, outpatient-, emergency-, non-emergency -admission numbers were collected. The relationship between inputs and outputs was modeled with the external input nonlinear autoregressive artificial neural network (ANN) (NARX) using MATLAB. Monthly test consumptions between January-July 2021 were used to test the models’ prediction power. Results: According to the cointegration analysis, total-, emergency-, and non-emergency admission numbers plus the number of working days per month were included in the model. When aPTT and fibrinogen consumptions were estimated, it was possible to predict the other tests. Fifty months of data were used to predict the next six months, and the NARX prediction was the more robust approach for both tests. Conclusions: The deep learning model gives better results than the intuitive approach in forecasting, even in the pandemic era, and it shows that more effective and efficient planning will be possible if ANN-supported decision mechanisms are used in forecasting tests’ consumptions in the procurement process.
{"title":"Forecasting The Consumptions of Coagulation Tests Using A Deep Learning Model","authors":"Banu Isbilen Basok, Ipek Deveci Kocakoç, Veli Iyilikci, Selena Kantarmaci, M. Fidan","doi":"10.5937/jomb0-40244","DOIUrl":"https://doi.org/10.5937/jomb0-40244","url":null,"abstract":"Background: Laboratory professionals aim to provide a reliable laboratory service using public resources efficiently while planning a test’s procurement. This intuitive approach is ineffective, as seen in the COVID-19 pandemic, where the dramatic changes in admissions (e.g. decreased patient admissions) and the purpose of testing (e.g. D-dimer) were experienced. A model based on objective data was developed that predicts the future test consumption of coagulation tests whose consumptions were highly variable during the pandemic. \u0000Methods: Between December 2018 and July 2021, monthly consumptions of coagulation tests (PTT, aPTT, D-dimer, fibrinogen), total-, inpatient-, outpatient-, emergency-, non-emergency -admission numbers were collected. The relationship between inputs and outputs was modeled with the external input nonlinear autoregressive artificial neural network (ANN) (NARX) using MATLAB. Monthly test consumptions between January-July 2021 were used to test the models’ prediction power. \u0000Results: According to the cointegration analysis, total-, emergency-, and non-emergency admission numbers plus the number of working days per month were included in the model. When aPTT and fibrinogen consumptions were estimated, it was possible to predict the other tests. Fifty months of data were used to predict the next six months, and the NARX prediction was the more robust approach for both tests. \u0000 \u0000Conclusions: The deep learning model gives better results than the intuitive approach in forecasting, even in the pandemic era, and it shows that more effective and efficient planning will be possible if ANN-supported decision mechanisms are used in forecasting tests’ consumptions in the procurement process.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"74 14","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138600288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To explore the variation of serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1) and squamous cell carcinoma (SCC) antigen in patients with lung cancer (LC) and their diagnostic value with endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). Methods: Selection of 150 patients with suspected lung malignant lesions as the research objects was to analyze the diagnostic value of serum tumor marker test and EBUS-TBNA joint detection for LC. Results: The AUC of joint examination to diagnose LC and identify pathological types of LC was elevated vs. alone detection of each index. Conclusions: Serum CYFRA21-1, SCC and CEA conjugating with EBUS-TBNA are provided with diagnostic value for LC and identified value for pathological types of LC.
{"title":"Analysis of variation of serum CEA, SCC, CYFRA21-1 in patients with lung cancer and their diagnostic value with EBUS-TBNA","authors":"YanJia Du, Ya Wen, JieYu Huang","doi":"10.5937/jomb0-37083","DOIUrl":"https://doi.org/10.5937/jomb0-37083","url":null,"abstract":"Objective: To explore the variation of serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1) and squamous cell carcinoma (SCC) antigen in patients with lung cancer (LC) and their diagnostic value with endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). Methods: Selection of 150 patients with suspected lung malignant lesions as the research objects was to analyze the diagnostic value of serum tumor marker test and EBUS-TBNA joint detection for LC. Results: The AUC of joint examination to diagnose LC and identify pathological types of LC was elevated vs. alone detection of each index. Conclusions: Serum CYFRA21-1, SCC and CEA conjugating with EBUS-TBNA are provided with diagnostic value for LC and identified value for pathological types of LC.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"8 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139238066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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