Jiang Wang, Ronghua Wang, Ying Zhou, Yao Ma, Chunyan Xiong
Background: To investigate the relationship between lactate dehydrogenase and apolipoprotein A1 levels and the condition and prognosis of patients with severe pneumonia.
Methods: We retrospectively collected 204 patients with severe pneumonia who were hospitalized from January 1, 2019 to December 1, 2021 in our hospital (respiratory intensive care unit (RICU)), and divided into survival group (160 patients) and death group (44 patients) according to their hospitalization outcome. The relationship between lactate dehydrogenase and apolipoprotein A1 levels and general information, disease, and treatment needs of patients with severe pneumonia was analyzed, and lactate dehydrogenase, apolipoprotein A1, neutrophil-to-lymphocyte ratio, hematocrit, C-reactive protein, calcitoninogen, D-dimer, Acute Physiology and Chronic Health Status Rating System II, and Pneumonia Severity Index scores were compared between the survival and death groups. The value of these indicators in determining the prognosis of patients was analyzed using subject operating characteristic (ROC) curves. Logistic regression was used to analyze the risk factors for death from severe pneumonia.
Results: The differences were statistically significant (P<0.05) when comparing age and pneumonia typing between the two groups. There was no statistically significant difference between the two groups in terms of gender and total length of stay (P >0.05). There was no statistically significant difference in LDH and ApoA1 levels between male patients and female patients (P>0.05). The differences in LDH and ApoA1 levels were statistically significant (P<0.05) when comparing patients with severe pneumonia at different ages. The differences in LDH and ApoA1 levels between SCAP and SHAP patients were not statistically significant (P>0.05). LDH and ApoA1 levels were higher in patients with severe pneumonia with acute exacerbation of slow-onset lung or MODS during hospitalization than in patients with severe pneumonia without acute exacerbation of slow-onset lung or MODS, with statistically significant differences (P<0.05). The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different PSI grades or APACHE II scores. The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different ICU length of stay. There was no statistically significant difference in LDH and ApoA1 levels when comparing patients with severe pneumonia who required tracheal intubation or sedation and analgesia during hospitalization (P>0.05). LDH and ApoA1 levels in patients with severe pneumonia with different duration of mechanical ventilation were compared with statistically significant differences (P<0.05). LDH and ApoA1 levels in the death group were 105.08 (75.22 ~140.0), which was significantly higher than 86.66 (62.66 ~ 106.14) in the survival group, with statisti
{"title":"The relationship between lactate dehydrogenase and apolipoprotein A1 levels in patients with severe pneumonia","authors":"Jiang Wang, Ronghua Wang, Ying Zhou, Yao Ma, Chunyan Xiong","doi":"10.5937/jomb0-45782","DOIUrl":"https://doi.org/10.5937/jomb0-45782","url":null,"abstract":"Background: To investigate the relationship between lactate dehydrogenase and apolipoprotein A1 levels and the condition and prognosis of patients with severe pneumonia.
 Methods: We retrospectively collected 204 patients with severe pneumonia who were hospitalized from January 1, 2019 to December 1, 2021 in our hospital (respiratory intensive care unit (RICU)), and divided into survival group (160 patients) and death group (44 patients) according to their hospitalization outcome. The relationship between lactate dehydrogenase and apolipoprotein A1 levels and general information, disease, and treatment needs of patients with severe pneumonia was analyzed, and lactate dehydrogenase, apolipoprotein A1, neutrophil-to-lymphocyte ratio, hematocrit, C-reactive protein, calcitoninogen, D-dimer, Acute Physiology and Chronic Health Status Rating System II, and Pneumonia Severity Index scores were compared between the survival and death groups. The value of these indicators in determining the prognosis of patients was analyzed using subject operating characteristic (ROC) curves. Logistic regression was used to analyze the risk factors for death from severe pneumonia.
 Results: The differences were statistically significant (P<0.05) when comparing age and pneumonia typing between the two groups. There was no statistically significant difference between the two groups in terms of gender and total length of stay (P >0.05). There was no statistically significant difference in LDH and ApoA1 levels between male patients and female patients (P>0.05). The differences in LDH and ApoA1 levels were statistically significant (P<0.05) when comparing patients with severe pneumonia at different ages. The differences in LDH and ApoA1 levels between SCAP and SHAP patients were not statistically significant (P>0.05). LDH and ApoA1 levels were higher in patients with severe pneumonia with acute exacerbation of slow-onset lung or MODS during hospitalization than in patients with severe pneumonia without acute exacerbation of slow-onset lung or MODS, with statistically significant differences (P<0.05). The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different PSI grades or APACHE II scores. The differences were statistically significant (P<0.05) when comparing LDH and ApoA1 levels in patients with severe pneumonia with different ICU length of stay. There was no statistically significant difference in LDH and ApoA1 levels when comparing patients with severe pneumonia who required tracheal intubation or sedation and analgesia during hospitalization (P>0.05). LDH and ApoA1 levels in patients with severe pneumonia with different duration of mechanical ventilation were compared with statistically significant differences (P<0.05). LDH and ApoA1 levels in the death group were 105.08 (75.22 ~140.0), which was significantly higher than 86.66 (62.66 ~ 106.14) in the survival group, with statisti","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":" 21","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135286390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Riccardo Nocini, Fabian Sanchis-Gomar, Giuseppe Lippi, Camilla Mattiuzzi
Background: This systematic literature review and meta-analysis investigated whether the red blood cell distribution (RDW) may predict survival outcomes in laryngeal cancer patients undergoing curative treatment.
Methods: We conducted an electronic search in Medline and Scopus using the keywords "red blood cell distribution width" OR "RDW" AND "laryngeal cancer" OR "larynx cancer" OR "laryngeal carcinoma" OR "larynx carcinoma," without time or language restrictions (up to February 2023), for identifying studies investigating the prognostic value of RDW in patients with any form of laryngeal cancer and with a primary endpoint that was set as survival rate and/or disease-free survival between 1 and 10 years after curative treatment. The research was conducted according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 reporting checklist.
{"title":"Red blood cell distribution width (RDW) is a significant predictor of survival in laryngeal cancer patients: Systematic literature review and meta-analysis.","authors":"Riccardo Nocini, Fabian Sanchis-Gomar, Giuseppe Lippi, Camilla Mattiuzzi","doi":"10.5937/jomb0-42947","DOIUrl":"10.5937/jomb0-42947","url":null,"abstract":"<p><strong>Background: </strong>This systematic literature review and meta-analysis investigated whether the red blood cell distribution (RDW) may predict survival outcomes in laryngeal cancer patients undergoing curative treatment.</p><p><strong>Methods: </strong>We conducted an electronic search in Medline and Scopus using the keywords \"red blood cell distribution width\" OR \"RDW\" AND \"laryngeal cancer\" OR \"larynx cancer\" OR \"laryngeal carcinoma\" OR \"larynx carcinoma,\" without time or language restrictions (up to February 2023), for identifying studies investigating the prognostic value of RDW in patients with any form of laryngeal cancer and with a primary endpoint that was set as survival rate and/or disease-free survival between 1 and 10 years after curative treatment. The research was conducted according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 reporting checklist.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"1 1","pages":"557-564"},"PeriodicalIF":2.5,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10712959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81512355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Backgroud: Carrier screening is the most effective method to block the occurrence of thalassemia. However, due to differences in race and genotype, MCV, MCH, HbA2 and other indicators are far from each other. The purpose of this study is to evaluate the common screening indicators of α, β and αβ-compound thalassemia carriers in Hunan Province, and try to use the relevant formulas in the existing literature to predict and distinguish different types of thalassemia carriers.
Methods: Receiver operating characteristic curve (ROC curve) combined with Youden index was utilized to analyze results of blood routine examination, hemoglobin electrophoresis, and literature-related formulas for 1111 α-thalassemia carriers, 464 β-thalassemia carriers and 24 αβ-thalassemia carriers.
Results: For α-thalassemia carriers, no matter which screening index or formula, the screening efficiency was not ideal. For β-thalassemia minor carriers, RBC, RDW_CV, Hb_A2, Hb_F and formula 5-7 could be used, and for compound thalassemia, RBC, RDW_CV, Hb_A2 and formula 5-6 are suitable. Hb_A2 has high efficiency in the screening of β-thalassemia minor and αβ-thalassemia. For the screening of β-thalassemia minor, if the cut-off value of HbA2 is set to 3%, the detection rate of 93.32% can be obtained at the positive rate of 9.6%, and if it is set to 3.15%, the detection rate can also reach 81.68% at the positive rate of 2.89%. For αβ-thalassemia, if the cut-off value of HbA2 is set to 3%, the detection rate of 95.83% can be obtained under the positive rate of 8.08%.
Conclusions: Different screening indicators and formulas have different efficiencies for different thalassemia carriers. α-thalassemia carriers are easily missed by screening indicators or corresponding formulas. HbA2 is a better screening indicator for both β-thalassemia minor carriers and αβ-thalassemia carriers, and formulas 5, 6, and 7 are suitable for β-thalassemia minor carriers, and formulas 5 and 6 are better for αβ-thalassemia carriers. To fully and objectively understand each screening index, data support has been provided for clinical and laboratory tests.
{"title":"Comparison of screening indicators for different types of thalassemia carriers in Hunan Province","authors":"Hua Tang, Rong Yu, ZiYin Yu, Hui Xi","doi":"10.5937/jomb0-46085","DOIUrl":"https://doi.org/10.5937/jomb0-46085","url":null,"abstract":"Backgroud: Carrier screening is the most effective method to block the occurrence of thalassemia. However, due to differences in race and genotype, MCV, MCH, HbA2 and other indicators are far from each other. The purpose of this study is to evaluate the common screening indicators of α, β and αβ-compound thalassemia carriers in Hunan Province, and try to use the relevant formulas in the existing literature to predict and distinguish different types of thalassemia carriers.
 Methods: Receiver operating characteristic curve (ROC curve) combined with Youden index was utilized to analyze results of blood routine examination, hemoglobin electrophoresis, and literature-related formulas for 1111 α-thalassemia carriers, 464 β-thalassemia carriers and 24 αβ-thalassemia carriers.
 Results: For α-thalassemia carriers, no matter which screening index or formula, the screening efficiency was not ideal. For β-thalassemia minor carriers, RBC, RDW_CV, Hb_A2, Hb_F and formula 5-7 could be used, and for compound thalassemia, RBC, RDW_CV, Hb_A2 and formula 5-6 are suitable. Hb_A2 has high efficiency in the screening of β-thalassemia minor and αβ-thalassemia. For the screening of β-thalassemia minor, if the cut-off value of HbA2 is set to 3%, the detection rate of 93.32% can be obtained at the positive rate of 9.6%, and if it is set to 3.15%, the detection rate can also reach 81.68% at the positive rate of 2.89%. For αβ-thalassemia, if the cut-off value of HbA2 is set to 3%, the detection rate of 95.83% can be obtained under the positive rate of 8.08%.
 Conclusions: Different screening indicators and formulas have different efficiencies for different thalassemia carriers. α-thalassemia carriers are easily missed by screening indicators or corresponding formulas. HbA2 is a better screening indicator for both β-thalassemia minor carriers and αβ-thalassemia carriers, and formulas 5, 6, and 7 are suitable for β-thalassemia minor carriers, and formulas 5 and 6 are better for αβ-thalassemia carriers. To fully and objectively understand each screening index, data support has been provided for clinical and laboratory tests.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"33 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135169371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hafize Uzun, Yagmur Ozge Turac Kosem, Mehmet Velidedeoglu, Pınar Kocael, Seyma Dumur, Osman Simsek
Background: Mammography, used for breast cancer (BC) screening, has limitations such as decreased sensitivity in dense breasts. Currently used tumor markers are insufficient in diagnosing breast cancer. In this study, we aimed to investigate the relationship between serum levels of synaptophysin-like protein 1 (SYPL1) and BC, as well as compare SYPL1 with other blood tumor markers. Method: The study group consisted of 80 female patients with a histopathological diagnosis of invasive BC and didn’t receive any radiotherapy/chemotherapy. The control group 72 women with noprevious history of breast disease and evaluated as Breast Imaging Reporting and Data Systems (BI-RADS 1-2) on imaging. Serum SYPL1, cancer antigen 15-3 (CA 15-3) and carcinoembryonic antigen (CEA) were measured in both groups. Results: The diagnostic values of SYPL1, CEA and CA15-3 proteins in diagnosing BC were statistically significant. The sensitivity of SYPL1 was 48.75%, with a specificity of 80.56%. CA15-3 had a sensitivity of 80% and a specificity of 49.30%. There wasn’t statistically significant correlation between serum SYPL1 and tumor diameter, lymph node metastasis, distant organ metastasis, and stage.
Conclusion: The serum SYPL1 maintained a higher discriminatory ability for BC. The serum SYPL1 level can be used with high specificity in the diagnosis of BC. Although SYPL1 has low diagnostic value in BC by itself.
背景:乳房x线摄影，用于乳腺癌筛查，有局限性，如在致密乳房敏感性降低。目前使用的肿瘤标志物对乳腺癌的诊断不足。在本研究中，我们旨在探讨血清突触素样蛋白1 (SYPL1)水平与BC的关系，并将SYPL1与其他血液肿瘤标志物进行比较。方法:研究组80例经组织病理学诊断为浸润性BC的女性患者，未接受任何放疗/化疗。对照组为72名既往无乳腺疾病病史的女性，采用乳腺影像学报告和数据系统(BI-RADS 1-2)进行影像学评估。检测两组患者血清SYPL1、癌抗原15-3 (CA 15-3)、癌胚抗原(CEA)水平。结果:SYPL1、CEA、CA15-3蛋白对BC的诊断价值有统计学意义。SYPL1的敏感性为48.75%，特异性为80.56%。CA15-3的敏感性为80%，特异性为49.30%。血清SYPL1与肿瘤直径、淋巴结转移、远处脏器转移、分期无统计学意义。& # x0D;结论:血清SYPL1对BC具有较高的鉴别能力。血清SYPL1水平可作为诊断BC的高特异性指标。虽然SYPL1本身对BC的诊断价值较低。
{"title":"The Clinical significance of serum synaptophysin like 1 protein levels in breast cancer","authors":"Hafize Uzun, Yagmur Ozge Turac Kosem, Mehmet Velidedeoglu, Pınar Kocael, Seyma Dumur, Osman Simsek","doi":"10.5937/jomb0-46198","DOIUrl":"https://doi.org/10.5937/jomb0-46198","url":null,"abstract":"Background: Mammography, used for breast cancer (BC) screening, has limitations such as decreased sensitivity in dense breasts. Currently used tumor markers are insufficient in diagnosing breast cancer. In this study, we aimed to investigate the relationship between serum levels of synaptophysin-like protein 1 (SYPL1) and BC, as well as compare SYPL1 with other blood tumor markers. Method: The study group consisted of 80 female patients with a histopathological diagnosis of invasive BC and didn’t receive any radiotherapy/chemotherapy. The control group 72 women with noprevious history of breast disease and evaluated as Breast Imaging Reporting and Data Systems (BI-RADS 1-2) on imaging. Serum SYPL1, cancer antigen 15-3 (CA 15-3) and carcinoembryonic antigen (CEA) were measured in both groups. Results: The diagnostic values of SYPL1, CEA and CA15-3 proteins in diagnosing BC were statistically significant. The sensitivity of SYPL1 was 48.75%, with a specificity of 80.56%. CA15-3 had a sensitivity of 80% and a specificity of 49.30%. There wasn’t statistically significant correlation between serum SYPL1 and tumor diameter, lymph node metastasis, distant organ metastasis, and stage. 
 Conclusion: The serum SYPL1 maintained a higher discriminatory ability for BC. The serum SYPL1 level can be used with high specificity in the diagnosis of BC. Although SYPL1 has low diagnostic value in BC by itself.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"11 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To study the roles of micro ribonucleic acid (miR)-155 host gene (MIR155HG) and tumor necrosis factor-α (TNF-α) in the evaluation of prognosis of patients with systemic lupus erythematosus (SLE).
Methods: A total of 130 patients with SLE admitted to our hospital were selected, and the SLE disease activity index (SLEDAI) score was given. The expressions of MIR155HG and TNF-α were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), the incidence of complications during treatment was observed, and the associations of MIR155HG and TNF-α with SLEDAI before treatment and complications were analyzed. All patients were followed up after discharge, and the related factors to the prognosis of patients were analyzed via Cox regression analysis.
Results: The levels of MIR155HG and TNF-α were higher in patients with an SLEDAI score of 10-14 points than those in patients with an SLEDAI score of 5-9 points and 0-4 points. MIR155HG and TNF-α were positively correlated with the incidence of infection, renal damage and cardiac damage. Moreover, there was also a positive correlation between the expressions of serum MIR155HG and TNF-α in SLE patients. SLEDAI score ≥10 points, complications during hospitalization, and highly-expressed MIR155HG and TNF-α were risk factors related to the prognosis of patients.
Conclusion: MIR155HG and TNF-α are key regulators in the pathogenesis of SLE, and they can affect the prognosis of patients. Such a finding provides potential new targets for the treatment of SLE.
{"title":"Roles of MIR155HG and TNF-α in Evaluation of Prognosis of Patients with Systemic Lupus Erythematosus","authors":"Xiaojing Gu, Hu Chen, Rongping Li, Dibin Guo","doi":"10.5937/jomb0-45870","DOIUrl":"https://doi.org/10.5937/jomb0-45870","url":null,"abstract":"Background: To study the roles of micro ribonucleic acid (miR)-155 host gene (MIR155HG) and tumor necrosis factor-α (TNF-α) in the evaluation of prognosis of patients with systemic lupus erythematosus (SLE).
 Methods: A total of 130 patients with SLE admitted to our hospital were selected, and the SLE disease activity index (SLEDAI) score was given. The expressions of MIR155HG and TNF-α were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), the incidence of complications during treatment was observed, and the associations of MIR155HG and TNF-α with SLEDAI before treatment and complications were analyzed. All patients were followed up after discharge, and the related factors to the prognosis of patients were analyzed via Cox regression analysis.
 Results: The levels of MIR155HG and TNF-α were higher in patients with an SLEDAI score of 10-14 points than those in patients with an SLEDAI score of 5-9 points and 0-4 points. MIR155HG and TNF-α were positively correlated with the incidence of infection, renal damage and cardiac damage. Moreover, there was also a positive correlation between the expressions of serum MIR155HG and TNF-α in SLE patients. SLEDAI score ≥10 points, complications during hospitalization, and highly-expressed MIR155HG and TNF-α were risk factors related to the prognosis of patients.
 Conclusion: MIR155HG and TNF-α are key regulators in the pathogenesis of SLE, and they can affect the prognosis of patients. Such a finding provides potential new targets for the treatment of SLE.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"49 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Multiple sclerosis is one of the most common demyelinating diseases of the central nervous system. We aimed to investigate serum and cerebrospinal fluid levels of different laboratory inflammatory biomarkers in patients with MS.
Methods: A total of 120 subjects participated in the study, 60 of whom are diagnosed with MS, 30 with the final diagnose of non-inflammatory diseases of the CNS, and 30 healthy subjects representing the control group. Regarding to progression of radiological findings after 2 years from the initial diagnosis, MS group was divided into subgroups: with stationary radiological findings (n=30) and with radiologically proven disease progression (n=30). In all patients we analyzed levels of laboratory inflammatory biomarkers: CRP, NLR, GDF15 and NFs. Values of NFs and GDF15 were analyzed initial while the values of CRP and NLR were analyzed initial and after two years.
Results: We found statistically lower GDF15 values and initial CRP values in MS group in regards to group with non-inflammatory diseases of the CNS (p=0.000). On the other side, we determined significant elevation of laboratory markers CRP and NLR, initial and after two years period, in MS subgroup with progression of MRI findings (p= 0.000 and p=0.050, respectively). Also we found a positive correlation of CRP and neurofilaments (r=0.243, p=0.04), as well as a positive correlation of CRP and GDF15 in patients with MS (r=0.769, p=0.000).
Conclusion: We found significant elevation of laboratory markers of systemic inflammation, CRP and NLR in MS patients who developed disease progression based on MRI findings.
Key words: Multiple sclerosis; Neutrophil to lymphocyte ratio; C reactive protein, Neurofilaments, Growth differentiation factor 15
{"title":"Determination of systemic inflamatory biomarkers in multiple sclerosis","authors":"Maša Todorović, Stanislava Nikolić, Željko Živanović, Svetlana Simić, Lorand Sakalaš, Igor Spasić, Branislava Ilinčić, Velibor Čabarkapa","doi":"10.5937/jomb0-45083","DOIUrl":"https://doi.org/10.5937/jomb0-45083","url":null,"abstract":"Background: Multiple sclerosis is one of the most common demyelinating diseases of the central nervous system. We aimed to investigate serum and cerebrospinal fluid levels of different laboratory inflammatory biomarkers in patients with MS.
 Methods: A total of 120 subjects participated in the study, 60 of whom are diagnosed with MS, 30 with the final diagnose of non-inflammatory diseases of the CNS, and 30 healthy subjects representing the control group. Regarding to progression of radiological findings after 2 years from the initial diagnosis, MS group was divided into subgroups: with stationary radiological findings (n=30) and with radiologically proven disease progression (n=30). In all patients we analyzed levels of laboratory inflammatory biomarkers: CRP, NLR, GDF15 and NFs. Values of NFs and GDF15 were analyzed initial while the values of CRP and NLR were analyzed initial and after two years.
 Results: We found statistically lower GDF15 values and initial CRP values in MS group in regards to group with non-inflammatory diseases of the CNS (p=0.000). On the other side, we determined significant elevation of laboratory markers CRP and NLR, initial and after two years period, in MS subgroup with progression of MRI findings (p= 0.000 and p=0.050, respectively). Also we found a positive correlation of CRP and neurofilaments (r=0.243, p=0.04), as well as a positive correlation of CRP and GDF15 in patients with MS (r=0.769, p=0.000).
 Conclusion: We found significant elevation of laboratory markers of systemic inflammation, CRP and NLR in MS patients who developed disease progression based on MRI findings.
 Key words: Multiple sclerosis; Neutrophil to lymphocyte ratio; C reactive protein, Neurofilaments, Growth differentiation factor 15","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135615987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To investigate the expression levels and diagnostic value of glial cell line-derived neurotrophic factor (GDNF), carcinoembryonic antigen (CEA) and carbohydrate antigen199 (CA199) in patients with colorectal carcinoma (CRC).
Methods. 50 CRC patients at our hospital from Feb. 2020 to Feb. 2021 were chosen as the malignant group, another 50 patients with benign colonic diseases were chosen as the benign group, and 50 healthy people who came to our hospital for physical examination during the same period were considered as the control group. Fasting peripheral venous blood was taken from all research subjects in the morning and tested by a fully-automated electrochemiluminometer to determine the GDNF, CEA and CA199 levels. The sensitivity and specificity of the combined detection of the three indexes for CRC were analyzed, and the ROC curve was plotted to record the area under the curve (AUC).
Results. The malignant group had remarkably higher CEA and CA199 levels (P<0.001) and a lower GDNF level (P<0.001) when compared with the benign and control groups. The sensitivity, specificity, positive predictive value and negative predictive value of the combined detection were 96.0%, 94.0%, 88.9% and 97.9%, respectively. The combined detection had the AUC (95% CI) = 0.950 (0.909-0.991), the standard error of 0.021, and the progressive Sig.b<0.001.
Conclusion. The combined diagnosis of serum GDNF, CEA and CA199 is a reliable method to improve the diagnostic accuracy of CRC, and this strategy can effectively reduce the missed diagnosis rate and has high application value in clinic.
{"title":"Expression levels and diagnostic value of serum GDNF, CEA and CA199 in patients with Colorectal Carcinoma","authors":"Jue Wang, Lulu Liu, Yan Zheng, Sai Gu","doi":"10.5937/jomb0-44745","DOIUrl":"https://doi.org/10.5937/jomb0-44745","url":null,"abstract":"Background: To investigate the expression levels and diagnostic value of glial cell line-derived neurotrophic factor (GDNF), carcinoembryonic antigen (CEA) and carbohydrate antigen199 (CA199) in patients with colorectal carcinoma (CRC). 
 Methods. 50 CRC patients at our hospital from Feb. 2020 to Feb. 2021 were chosen as the malignant group, another 50 patients with benign colonic diseases were chosen as the benign group, and 50 healthy people who came to our hospital for physical examination during the same period were considered as the control group. Fasting peripheral venous blood was taken from all research subjects in the morning and tested by a fully-automated electrochemiluminometer to determine the GDNF, CEA and CA199 levels. The sensitivity and specificity of the combined detection of the three indexes for CRC were analyzed, and the ROC curve was plotted to record the area under the curve (AUC). 
 Results. The malignant group had remarkably higher CEA and CA199 levels (P<0.001) and a lower GDNF level (P<0.001) when compared with the benign and control groups. The sensitivity, specificity, positive predictive value and negative predictive value of the combined detection were 96.0%, 94.0%, 88.9% and 97.9%, respectively. The combined detection had the AUC (95% CI) = 0.950 (0.909-0.991), the standard error of 0.021, and the progressive Sig.b<0.001. 
 Conclusion. The combined diagnosis of serum GDNF, CEA and CA199 is a reliable method to improve the diagnostic accuracy of CRC, and this strategy can effectively reduce the missed diagnosis rate and has high application value in clinic.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Geng, Jinli Shi, Weina Guo, Haiyan Li, Dan Yang, Yan Gao
Background: It is an important clinical means to identify benign and malignant breast diseases caused by nipple discharge through the detection and analysis of components in nipple discharge. This study was aimed to test the expression and clinical significance of carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153) and carcinoembryonic antigen (CEA) in nipple discharge of breast cancer patients.
Methods: From January 2017 to December 2018, 86 patients with invasive ductal carcinoma of the breast with nipple discharge (breast cancer group) and 50 patients with ordinary breast duct hyperplasia with nipple discharge (benign control group) were selected, and the nipple discharge and serum CA125, CA153 and CEA levels in the two groups were detected by electrochemiluminescence immunoassay.
Results: The nipple discharge and serum CA125, CA153 and CEA levels in the benign control group were significantly lower than those in the breast cancer group, and the serum CA125, CA153 and CEA levels were obviously lower than those nipple discharge levels. The expression levels of CA125, CA153 and CEA in nipple discharge in the breast cancer group had no significant difference in different age of onset and different tumor sites. The CA125, CA153 and CEA levels in nipple discharge of patients with tumor diameter n5 cm, low differentiation, high stage, metastasis, and recurrence were obviously elevated versus to those of patients with tumor diameter <5 cm, high differentiation, low stage, and no metastasis and recurrence. These levels were not significantly correlated with the expression of estrogen receptor (ER) and progesterone receptor (PR), but was significantly correlated with the expression of human epidermal growth factor receptor (HER-2) and Ki-67. The accuracy, sensitivity, and negative predictive value of nipple discharge CA125, CA153 and CEA combined tests in the diagnosis of breast cancer were markedly improved compared with serum combinations and individual tests.
Conclusion: The combination of CA125, CA153 and CEA in nipple discharge can be considered as a potential diagnostic method for breast cancer, which is an effective supplement to serological diagnosis, and can provide new ideas for the differential diagnosis of benign and malignant breast cancer with nipple discharge.
{"title":"Expression and clinical significance of CA125, CA153 and CEA in nipple discharge of breast cancer patients","authors":"Jun Geng, Jinli Shi, Weina Guo, Haiyan Li, Dan Yang, Yan Gao","doi":"10.5937/jomb0-45192","DOIUrl":"https://doi.org/10.5937/jomb0-45192","url":null,"abstract":"Background: It is an important clinical means to identify benign and malignant breast diseases caused by nipple discharge through the detection and analysis of components in nipple discharge. This study was aimed to test the expression and clinical significance of carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153) and carcinoembryonic antigen (CEA) in nipple discharge of breast cancer patients. 
 Methods: From January 2017 to December 2018, 86 patients with invasive ductal carcinoma of the breast with nipple discharge (breast cancer group) and 50 patients with ordinary breast duct hyperplasia with nipple discharge (benign control group) were selected, and the nipple discharge and serum CA125, CA153 and CEA levels in the two groups were detected by electrochemiluminescence immunoassay.
 Results: The nipple discharge and serum CA125, CA153 and CEA levels in the benign control group were significantly lower than those in the breast cancer group, and the serum CA125, CA153 and CEA levels were obviously lower than those nipple discharge levels. The expression levels of CA125, CA153 and CEA in nipple discharge in the breast cancer group had no significant difference in different age of onset and different tumor sites. The CA125, CA153 and CEA levels in nipple discharge of patients with tumor diameter n5 cm, low differentiation, high stage, metastasis, and recurrence were obviously elevated versus to those of patients with tumor diameter <5 cm, high differentiation, low stage, and no metastasis and recurrence. These levels were not significantly correlated with the expression of estrogen receptor (ER) and progesterone receptor (PR), but was significantly correlated with the expression of human epidermal growth factor receptor (HER-2) and Ki-67. The accuracy, sensitivity, and negative predictive value of nipple discharge CA125, CA153 and CEA combined tests in the diagnosis of breast cancer were markedly improved compared with serum combinations and individual tests.
 Conclusion: The combination of CA125, CA153 and CEA in nipple discharge can be considered as a potential diagnostic method for breast cancer, which is an effective supplement to serological diagnosis, and can provide new ideas for the differential diagnosis of benign and malignant breast cancer with nipple discharge.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136308928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To assess the influence of serum level of YAP on laboratory examination findings, imaging findings and disease activity of rheumatoid arthritis patients combined cardiovascular disease (RA-CVD).
Methods: RA-CVD patients (n=60), RA-nCVD patients (n=60) and healthy subjects (n=60) were recruited. Serum levels of YAP in them were detected by qRT-PCR. Their baseline characteristics were analyzed and compared. Disease activity, CVD risk factors and imaging findings in RA-CVD and RA-nCVD patients were evaluated and compared. In addition, potential influences of YAP on disease activity, CVD risk factors and imaging findings in RA-CVD patients were assessed.
Results: RA-CVD patients had higher levels of ERS, anti-CCP, RF, HDL-C, CRP, FRS, BNP, LA, LVs, LVd and cIMT, and lower level of EF in comparison to RA-nCVD patients. Serum level of YAP was higher in RA-CVD patients than that of RA-nCVD patients and healthy subjects. YAP level was positively correlated to DAS28, TG, CRP, PLT, FRS, BNP and cIMT in RA-CVD patients.
Conclusion: Serum level of YAP increases in RA-CVD patients. YAP is a potential factor driving the development of CVD in RA patients through regulating inflammatory response, lipid metabolism, glycometabolism and thrombosis.
{"title":"YAP drives the development of cardiovascular disease in patients with rheumatoid arthritis","authors":"Guozhu Che, Ying Liu, Na Zhang, Jing Zhao","doi":"10.5937/jomb0-45932","DOIUrl":"https://doi.org/10.5937/jomb0-45932","url":null,"abstract":"Background: To assess the influence of serum level of YAP on laboratory examination findings, imaging findings and disease activity of rheumatoid arthritis patients combined cardiovascular disease (RA-CVD). 
 Methods: RA-CVD patients (n=60), RA-nCVD patients (n=60) and healthy subjects (n=60) were recruited. Serum levels of YAP in them were detected by qRT-PCR. Their baseline characteristics were analyzed and compared. Disease activity, CVD risk factors and imaging findings in RA-CVD and RA-nCVD patients were evaluated and compared. In addition, potential influences of YAP on disease activity, CVD risk factors and imaging findings in RA-CVD patients were assessed. 
 Results: RA-CVD patients had higher levels of ERS, anti-CCP, RF, HDL-C, CRP, FRS, BNP, LA, LVs, LVd and cIMT, and lower level of EF in comparison to RA-nCVD patients. Serum level of YAP was higher in RA-CVD patients than that of RA-nCVD patients and healthy subjects. YAP level was positively correlated to DAS28, TG, CRP, PLT, FRS, BNP and cIMT in RA-CVD patients. 
 Conclusion: Serum level of YAP increases in RA-CVD patients. YAP is a potential factor driving the development of CVD in RA patients through regulating inflammatory response, lipid metabolism, glycometabolism and thrombosis.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136309098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: to observe the basic metabolic characteristics of obese patients with PCOS, and observe and compare the effect of laparoscopic sleeve gastrectomy and metformin treatment after 3 months.
Methods: In January to December 2018, the Second Hospital of Hebei Medical University selected 104 women who were classified as obese with a BMI of 28kg/cm2 or higher and had PCOS. They were divided into obese PCOS group (53 cases) and obese non-pcos group (51 cases).
Results: 1. There was no significant difference in waist circumference and WHR between patients who are obese with PCOS and patients who are obese without PCOS (P > 0.05).Obese PCOS patients were significantly higher in AMH, LH/FSH, T, FAI, homa-ir, TG, LDL, apo-b and uric acid than the group of non-PCOS patients who were obese. (P<0.05). The SHBG levels of obese patients with PCOS were obviously lower when contrasted with the levels in obese patients without PCOS (P < 0.05). 2. Body weight, BMI, INS, homa-ir and TG of obese PCOS patients were significantly decreased 3 months after laparoscopic sleeve gastrectomy compared with that before surgery (P < 0.05). After three months of medical treatment with metformin, the patients' HOMA-IR was obviously reduced when contrasted with the pre-treatment HOMA-IR levels (P < 0.05), and there was no significant difference in the improvement degree of homa-ir between the two groups (P > 0.05).
Conclusion: 1. Obese patients with PCOS demonstrated higher expression of AMH, LH/FSH, T, SHBG, and FAI when contrasted with the control group. Additionally, they experienced more severe insulin resistance and lipid metabolism disorders. 2. The weight and BMI of obese PCOS patients were significantly decreased after weight loss, while IR and blood lipid were significantly improved, while IR was improved in metformin group, and no significant discrepancy was observed in the degree of improvement of insulin resistance between both groups.
{"title":"Clinical observation of laparoscopic sleeve gastrectomy and metformin treatment in obese PCOS patients","authors":"Qingya Ma, Xiaojing He, Zijie Fu, Xiaodong Ren, Ranran Sun, Siqi Zhu, Xiaodong Li, Yahui Bian","doi":"10.5937/jomb0-44411","DOIUrl":"https://doi.org/10.5937/jomb0-44411","url":null,"abstract":"Background: to observe the basic metabolic characteristics of obese patients with PCOS, and observe and compare the effect of laparoscopic sleeve gastrectomy and metformin treatment after 3 months.
 Methods: In January to December 2018, the Second Hospital of Hebei Medical University selected 104 women who were classified as obese with a BMI of 28kg/cm2 or higher and had PCOS. They were divided into obese PCOS group (53 cases) and obese non-pcos group (51 cases).
 Results: 1. There was no significant difference in waist circumference and WHR between patients who are obese with PCOS and patients who are obese without PCOS (P > 0.05).Obese PCOS patients were significantly higher in AMH, LH/FSH, T, FAI, homa-ir, TG, LDL, apo-b and uric acid than the group of non-PCOS patients who were obese. (P<0.05). The SHBG levels of obese patients with PCOS were obviously lower when contrasted with the levels in obese patients without PCOS (P < 0.05). 2. Body weight, BMI, INS, homa-ir and TG of obese PCOS patients were significantly decreased 3 months after laparoscopic sleeve gastrectomy compared with that before surgery (P < 0.05). After three months of medical treatment with metformin, the patients' HOMA-IR was obviously reduced when contrasted with the pre-treatment HOMA-IR levels (P < 0.05), and there was no significant difference in the improvement degree of homa-ir between the two groups (P > 0.05).
 Conclusion: 1. Obese patients with PCOS demonstrated higher expression of AMH, LH/FSH, T, SHBG, and FAI when contrasted with the control group. Additionally, they experienced more severe insulin resistance and lipid metabolism disorders. 2. The weight and BMI of obese PCOS patients were significantly decreased after weight loss, while IR and blood lipid were significantly improved, while IR was improved in metformin group, and no significant discrepancy was observed in the degree of improvement of insulin resistance between both groups.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135785789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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