首页 > 最新文献

Journal of Medical Biochemistry最新文献

英文 中文
DIFFERENT DOSES OF RECOMBINANT FSH AND DETERMINING PARAMETERS OF OXIDATIVE STRESS 不同剂量重组卵泡刺激素对氧化应激参数的影响
4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-09-11 DOI: 10.5937/jomb0-46156
Lidija Tulić, None Ivan, Jelena Stojnic, Jovan Bila, Zeljka Vukovic, Boba Kotlica
Background: This study aimed to examine if there is a connection between recombinant FSH dose and OS parameters in serum and the impact on IVF outcome. Methods: This study consisted of 101 participants that went through IVF procedures. Parameter that were tested are SOD, SH groups and MDA.  Serum samples were drawn before stimulation and on the last day of ovarian stimulation. Results: Two groups were formed according to the dose of gonadotropins (rFSH). In both groups there were no significant differences in live–birth rate and miscarriage. In both groups mean serum MDA and SH-groups were significantly higher after ovarian stimulation, but mean serum SOD was significantly lower when compared to values before stimulation. There were less patients without OS before stimulation. Conclusion: Our results suggest that there is a difference in serum concentration in groups SOD, SH groups and MDA at the beginning and at the end ovarian stimulation. On the other hand, dose of rFSH is not related with change of parameters for oxidative stress, quality of oocytes, embryos, fertilization, pregnancies, and miscarriage rate. Patients without oxidative stress before the IVF procedure needed lower doses of gonadotropins during stimulation. Keywords: in vitro fertilization, oxidative stress, gonadotropin dose
背景:本研究旨在探讨血清中重组卵泡刺激素(FSH)剂量和OS参数与体外受精(IVF)结果的影响之间是否存在联系。& # x0D;方法:本研究包括101名接受体外受精手术的参与者。检测SOD、SH组和MDA。在刺激前和卵巢刺激最后一天抽取血清样本。& # x0D;结果:按促性腺激素(rFSH)剂量分为两组。两组的活产率和流产率无显著差异。两组患者卵巢刺激后血清MDA和sh均显著高于刺激前,血清SOD均显著低于刺激前。刺激前无OS的患者较少。 结论:卵巢刺激开始和结束时,血清SOD、SH和MDA浓度存在差异。另一方面,rFSH剂量与氧化应激、卵母细胞质量、胚胎、受精、妊娠和流产率等参数的变化无关。体外受精手术前无氧化应激的患者在刺激期间需要较低剂量的促性腺激素。 关键词:体外受精;氧化应激;促性腺激素;
{"title":"DIFFERENT DOSES OF RECOMBINANT FSH AND DETERMINING PARAMETERS OF OXIDATIVE STRESS","authors":"Lidija Tulić, None Ivan, Jelena Stojnic, Jovan Bila, Zeljka Vukovic, Boba Kotlica","doi":"10.5937/jomb0-46156","DOIUrl":"https://doi.org/10.5937/jomb0-46156","url":null,"abstract":"Background: This study aimed to examine if there is a connection between recombinant FSH dose and OS parameters in serum and the impact on IVF outcome. \u0000Methods: This study consisted of 101 participants that went through IVF procedures. Parameter that were tested are SOD, SH groups and MDA.  Serum samples were drawn before stimulation and on the last day of ovarian stimulation. \u0000Results: Two groups were formed according to the dose of gonadotropins (rFSH). In both groups there were no significant differences in live–birth rate and miscarriage. In both groups mean serum MDA and SH-groups were significantly higher after ovarian stimulation, but mean serum SOD was significantly lower when compared to values before stimulation. There were less patients without OS before stimulation. \u0000Conclusion: Our results suggest that there is a difference in serum concentration in groups SOD, SH groups and MDA at the beginning and at the end ovarian stimulation. On the other hand, dose of rFSH is not related with change of parameters for oxidative stress, quality of oocytes, embryos, fertilization, pregnancies, and miscarriage rate. Patients without oxidative stress before the IVF procedure needed lower doses of gonadotropins during stimulation. \u0000Keywords: in vitro fertilization, oxidative stress, gonadotropin dose","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136023557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association between triglyceride-glucose index and acute kidney injury in patients with acute myocardial infarction based on Medical Information Mart for Intensive Care database: A cross-sectional study 基于重症监护医学信息集市数据库的急性心肌梗死患者甘油三酯-葡萄糖指数与急性肾损伤的相关性横断面研究
4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-30 DOI: 10.5937/jomb0-45219
Zihan Jin, Lu Xiao, Xinyi Xu, Changhong Miao, Yi Liu
Background: The relationship between triglyceride glucose (TyG) index and the incidence of acute kidney injury (AKI) in patients with acute myocardial infarction (AMI) is unclear. This study aims to explore the relationship between the two. Methods: Participants were enrolled from Medical Information Mart for Intensive Care IV (MIMICIV) and grouping of subjects based on the quartile interval of the TyG index. With the presence of AKI as the main outcome, a logistic regression model was constructed. The correlation of the TyG index with the results obtained was examined by using a restricted cubic spline (RCS) model. Results: Among the 1,101 AMI participants, 64.7% were male patients and the overall incidence rate of AKI was 37.1%. The results of multivariate Logistic regression analysis (LRA) revealed independent correlation of the TyG index with increased AKI risk (odds ratio (OR) = 2.23; confidence interval (CI) = 1.59 to 3.17; P < 0.001). Q4 (9.5-12.208) in the TyG index quartile was independently correlated with increased AKI risk (OR = 2.99, 95% CI = 1.64 to 5.46, P < 0.001). The RCS model showed that the AKI risk increased linearly as the TyG index increased (P=0.313). Conclusion: In patients with AMI, the incidence of TyG and AKI is closely related. Nevertheless, further studies are needed to verify the finding.
背景:急性心肌梗死(AMI)患者甘油三酯葡萄糖(TyG)指数与急性肾损伤(AKI)发生率的关系尚不清楚。本研究旨在探讨两者之间的关系。 方法:从重症监护医学信息市场(MIMICIV)中招募受试者,根据TyG指数的四分位数区间对受试者进行分组。以AKI的存在为主要结果,构建logistic回归模型。使用限制三次样条(RCS)模型检验了TyG指数与所得结果的相关性。 结果:1101例AMI患者中,男性占64.7%,AKI总发病率为37.1%。多因素Logistic回归分析(LRA)结果显示TyG指数与AKI风险增加存在独立相关性(优势比(OR) = 2.23;置信区间(CI) = 1.59 ~ 3.17;P & lt;0.001)。TyG指数四分位数中的Q4(9.5-12.208)与AKI风险增加独立相关(OR = 2.99, 95% CI = 1.64 ~ 5.46, P <0.001)。RCS模型显示,随着TyG指数的增加,AKI风险呈线性增加(P=0.313)。 结论:在AMI患者中,TyG与AKI的发生率密切相关。然而,需要进一步的研究来证实这一发现。
{"title":"Association between triglyceride-glucose index and acute kidney injury in patients with acute myocardial infarction based on Medical Information Mart for Intensive Care database: A cross-sectional study","authors":"Zihan Jin, Lu Xiao, Xinyi Xu, Changhong Miao, Yi Liu","doi":"10.5937/jomb0-45219","DOIUrl":"https://doi.org/10.5937/jomb0-45219","url":null,"abstract":"Background: The relationship between triglyceride glucose (TyG) index and the incidence of acute kidney injury (AKI) in patients with acute myocardial infarction (AMI) is unclear. This study aims to explore the relationship between the two.&#x0D; Methods: Participants were enrolled from Medical Information Mart for Intensive Care IV (MIMICIV) and grouping of subjects based on the quartile interval of the TyG index. With the presence of AKI as the main outcome, a logistic regression model was constructed. The correlation of the TyG index with the results obtained was examined by using a restricted cubic spline (RCS) model.&#x0D; Results: Among the 1,101 AMI participants, 64.7% were male patients and the overall incidence rate of AKI was 37.1%. The results of multivariate Logistic regression analysis (LRA) revealed independent correlation of the TyG index with increased AKI risk (odds ratio (OR) = 2.23; confidence interval (CI) = 1.59 to 3.17; P < 0.001). Q4 (9.5-12.208) in the TyG index quartile was independently correlated with increased AKI risk (OR = 2.99, 95% CI = 1.64 to 5.46, P < 0.001). The RCS model showed that the AKI risk increased linearly as the TyG index increased (P=0.313).&#x0D; Conclusion: In patients with AMI, the incidence of TyG and AKI is closely related. Nevertheless, further studies are needed to verify the finding.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136144039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of the effect of vitamin K1 prophylaxis on newborn screenings tests in newborns. 维生素K1预防在新生儿筛查中的作用研究。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-40162
Murat Caglayan, Ataman Gonel, Cuneyt Tayman, Ufuk Cakir, Ismail Koyuncu, Ebru Temiz, Yasemin Sert

Background: Routine screening for hereditary disorders in newborns includes screening for treatable metabolic and endocrine disorders, such as biotidinase deficiency, galactosemia, maple syrup urine disease, hypothyroidism, and cystic fibrosis. Incorrect test results may be encountered due to the use of vitamin K1. To investigate the interference effect of vitamin K1 on neonatal screening tests and to raise awareness of erroneous measurements.

Methods: Heel blood samples were taken from 25 newborns born in a neonatal intensive care unit. Dry blood C0, C2, C3, C4, C4DC, C5:1, C5OH, C5DC, C6, C6DC, C8, C8:1, C8DC, C10, C10:1, C10DC, C12, C14, C14:1, C14:2, C16, C16:1, C18, C18:1, C18:2, C18:OH, methylglutaryl, valine, leucine/isoleucine, methionine, phenylalanine, argininosuccinic acid, aspartate, alanine, arginine, citrulline, glycine, ornithine, and glutamate tests were studied using the tandem mass spectrometry (MS) method. The results of the heel blood samples obtained before and after the application of vitamin K1 (Phyto menadione) were compared.

Results: In two studies conducted with in vitro and in vivo tests, C0, C2, C3, C4, C4DC, C5, C5OH, C6, C8, C10, C10:1, C14, C16, C16:1, C18, C18:1, methylglutaryl, phenylalanine, argininosuccinic acid, tyrosine, aspartate, arginine, citrulline, glycine, and glutamine were all significantly elevated (p < 0.05).

Conclusions: Heel blood samples may yield false results due to vitamin K1 administration. In the case of doubtful results, a new sample should be taken and the measurement should be repeated.

背景:新生儿遗传性疾病的常规筛查包括可治疗的代谢和内分泌疾病的筛查,如生物素酶缺乏、半乳糖血症、枫糖浆尿病、甲状腺功能减退和囊性纤维化。由于使用维生素K1,可能会遇到不正确的测试结果。研究维生素K1对新生儿筛查测试的干扰作用,并提高对错误测量的认识。方法:从新生儿重症监护室出生的25名新生儿身上采集足跟血样本。干血C0、C2、C3、C4、C4DC、C5:1、C5OH、C5DC、C6、C6DC、C8、C8:1、C8DC、C10、C10:1、C10DC、C12、C14、C14:1、C14:2、C16、C16:1、C18、C18:1、C18:2、C18:OH、甲基戊二酰、缬氨酸、亮氨酸/异亮氨酸、甲硫氨酸、苯丙氨酸、精氨酸、天冬氨酸、丙氨酸、精氨酸酯、瓜氨酸、甘氨酸、鸟氨酸和谷氨酸测试采用串联质谱法进行研究。比较在施用维生素K1(Phyto menadione)之前和之后获得的足跟血液样本的结果。结果:在两项体外和体内试验研究中,C0、C2、C3、C4、C4DC、C5、C5OH、C6、C8、C10、C10:1、C14、C16、C16:1、C18、C18:1、甲基戊二酰、苯丙氨酸、精氨酸,谷氨酰胺均显著升高(p<0.05)。如果结果可疑,则应重新取样并重复测量。
{"title":"Investigation of the effect of vitamin K1 prophylaxis on newborn screenings tests in newborns.","authors":"Murat Caglayan,&nbsp;Ataman Gonel,&nbsp;Cuneyt Tayman,&nbsp;Ufuk Cakir,&nbsp;Ismail Koyuncu,&nbsp;Ebru Temiz,&nbsp;Yasemin Sert","doi":"10.5937/jomb0-40162","DOIUrl":"10.5937/jomb0-40162","url":null,"abstract":"<p><strong>Background: </strong>Routine screening for hereditary disorders in newborns includes screening for treatable metabolic and endocrine disorders, such as biotidinase deficiency, galactosemia, maple syrup urine disease, hypothyroidism, and cystic fibrosis. Incorrect test results may be encountered due to the use of vitamin K1. To investigate the interference effect of vitamin K1 on neonatal screening tests and to raise awareness of erroneous measurements.</p><p><strong>Methods: </strong>Heel blood samples were taken from 25 newborns born in a neonatal intensive care unit. Dry blood C0, C2, C3, C4, C4DC, C5:1, C5OH, C5DC, C6, C6DC, C8, C8:1, C8DC, C10, C10:1, C10DC, C12, C14, C14:1, C14:2, C16, C16:1, C18, C18:1, C18:2, C18:OH, methylglutaryl, valine, leucine/isoleucine, methionine, phenylalanine, argininosuccinic acid, aspartate, alanine, arginine, citrulline, glycine, ornithine, and glutamate tests were studied using the tandem mass spectrometry (MS) method. The results of the heel blood samples obtained before and after the application of vitamin K1 (Phyto menadione) were compared.</p><p><strong>Results: </strong>In two studies conducted with in vitro and in vivo tests, C0, C2, C3, C4, C4DC, C5, C5OH, C6, C8, C10, C10:1, C14, C16, C16:1, C18, C18:1, methylglutaryl, phenylalanine, argininosuccinic acid, tyrosine, aspartate, arginine, citrulline, glycine, and glutamine were all significantly elevated (p < 0.05).</p><p><strong>Conclusions: </strong>Heel blood samples may yield false results due to vitamin K1 administration. In the case of doubtful results, a new sample should be taken and the measurement should be repeated.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"376-382"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of the relationship between endothelial nitric oxide synthase T786C polymorphism and PSA, PSA derivatives, and prostate cancer in the Turkish population. 土耳其人群内皮型一氧化氮合酶T786C多态性与PSA、PSA衍生物和前列腺癌症关系的研究。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-33122
Senay Balci, Serin Akbayir, Murat Bozlu, Lulufer Tamer

Background: Prostate cancer is a slowly progressing cancer. However, it has remained a major medical problem for affected men. Risk factors of prostate cancer include age, race, and prostate cancer family history. Prostate cancer may occur at different frequencies between ethnic populations and countries. Currently, studies on genetic risk factors in prostate cancer aetiology have been increasing. Due to the importance of changes in endothelial nitric oxide synthase in carcinogenesis, we aimed to reveal whether eNOS T786C polymorphism is associated with prostate cancer.

Methods: Archival samples included in this study were whole blood samples taken from patients who were grouped according to prostate biopsy pathology results (BPH, n: 42; PCa, n: 48) and from healthy participants (controls, n:27). DNA was isolated from these whole blood samples and real-time polymerase chain reaction analysis was performed for endothelial nitric oxide synthase T786C polymorphism with LightCycler 480 II. Measured free and total prostate-specific antigen serum levels were evaluated retrospectively.

Results: There was a statistical difference between patient-healthy control and control-healthy control groups regarding genotype distributions for eNOS T786C hism. Controls were more likely to have TC and CC genotypes and C alleles than the other two groups.

Conclusions: Compared to other groups, the percentage of the eNOS786C allele in the control group was found to be higher. As a result of these data, it can be thought that carrying the allele may be protective against the disease.

背景:前列腺癌症是一种进展缓慢的癌症。然而,它仍然是受影响男性的一个主要医疗问题。前列腺癌症的危险因素包括年龄、种族和癌症家族史。前列腺癌症可能在不同民族和国家之间发生不同的频率。目前,对癌症病因中遗传危险因素的研究日益增多。由于内皮型一氧化氮合酶的变化在癌症发生中的重要性,我们旨在揭示eNOS T786C多态性是否与前列腺癌症相关。方法:本研究中包括的档案样本是根据前列腺活检病理结果分组的患者(BPH,n:42;前列腺癌,n:48)和健康参与者(对照组,n:27)的全血样本。从这些全血样品中分离DNA,并用LightCycler480II对内皮一氧化氮合酶T786C多态性进行实时聚合酶链式反应分析。对测量的游离和总前列腺特异性抗原血清水平进行回顾性评估。结果:在eNOS T786C hism基因型分布方面,患者健康对照组和对照健康对照组之间存在统计学差异。对照组比其他两组更有可能具有TC和CC基因型以及C等位基因。结论:与其他组相比,对照组eNOS786C等位基因的百分比更高。根据这些数据,可以认为携带等位基因可能对疾病具有保护作用。
{"title":"Investigation of the relationship between endothelial nitric oxide synthase T786C polymorphism and PSA, PSA derivatives, and prostate cancer in the Turkish population.","authors":"Senay Balci,&nbsp;Serin Akbayir,&nbsp;Murat Bozlu,&nbsp;Lulufer Tamer","doi":"10.5937/jomb0-33122","DOIUrl":"10.5937/jomb0-33122","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer is a slowly progressing cancer. However, it has remained a major medical problem for affected men. Risk factors of prostate cancer include age, race, and prostate cancer family history. Prostate cancer may occur at different frequencies between ethnic populations and countries. Currently, studies on genetic risk factors in prostate cancer aetiology have been increasing. Due to the importance of changes in endothelial nitric oxide synthase in carcinogenesis, we aimed to reveal whether eNOS T786C polymorphism is associated with prostate cancer.</p><p><strong>Methods: </strong>Archival samples included in this study were whole blood samples taken from patients who were grouped according to prostate biopsy pathology results (BPH, n: 42; PCa, n: 48) and from healthy participants (controls, n:27). DNA was isolated from these whole blood samples and real-time polymerase chain reaction analysis was performed for endothelial nitric oxide synthase T786C polymorphism with LightCycler 480 II. Measured free and total prostate-specific antigen serum levels were evaluated retrospectively.</p><p><strong>Results: </strong>There was a statistical difference between patient-healthy control and control-healthy control groups regarding genotype distributions for eNOS T786C hism. Controls were more likely to have TC and CC genotypes and C alleles than the other two groups.</p><p><strong>Conclusions: </strong>Compared to other groups, the percentage of the eNOS786C allele in the control group was found to be higher. As a result of these data, it can be thought that carrying the allele may be protective against the disease.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"357-363"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The predictive role of biochemical markers on outcomes of severe COVID-19 patients admitted to intensive care unit. 生化标志物对重症监护病房收治的重症新冠肺炎患者预后的预测作用。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-40641
Bosa Mirjanić-Azarić, Ivana Pejić, Smiljana Mijić, Aleksandra Pejčić, Anita Đurđević-Svraka, Dragan Svraka, Darija Knežević, Tatjana Milivojac, Nataša Bogavac-Stanojević

Background: The pandemic of severe acute respiratory syndrome by coronavirus 2 (SARS-CoV-2) is a multi-system disease caused by a diffuse systemic process involving a complex interaction of the inflammatory, immunological and coagulative cascades. This study aims to identify the most effective biomarkers to predict poor outcome in intensive care unit (ICU) patients with severe COVID-19 disease.

Methods: A single-centre retrospective observational study enrolled 69 deceased and 20 recovered patients treated in the ICU of the General Hospital Gradiska in the period from March 1, 2021. until April 1, 2022. We evaluated the leukocytes (WBC), lymphocytes (LYM), neutrophils (NEU), platelets (PLT), haemoglobin, neutrophil-lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and systemic immune-inflammation index (SII). In addition, we evaluated the IL-6, ferritin, CRP, D-dimer, magnesium, bilirubin and lactate dehydrogenase.

背景:冠状病毒2型严重急性呼吸系统综合征(严重急性呼吸综合征冠状病毒2型)是一种多系统疾病,由炎症、免疫和凝血级联反应的复杂相互作用引起。本研究旨在确定最有效的生物标志物,以预测重症新冠肺炎重症监护室(ICU)患者的不良结局。方法:一项单中心回顾性观察性研究纳入了自2021年3月1日起在格拉迪斯卡综合医院ICU接受治疗的69名死者和20名康复患者。至2022年4月1日。我们评估了白细胞(WBC)、淋巴细胞(LYM)、嗜中性粒细胞(NEU)、血小板(PLT)、血红蛋白、中性粒细胞淋巴细胞比率(NLR)、血小板淋巴细胞比率(PLR)和全身免疫炎症指数(SII)。此外,我们还评估了IL-6、铁蛋白、CRP、D-二聚体、镁、胆红素和乳酸脱氢酶。
{"title":"The predictive role of biochemical markers on outcomes of severe COVID-19 patients admitted to intensive care unit.","authors":"Bosa Mirjanić-Azarić,&nbsp;Ivana Pejić,&nbsp;Smiljana Mijić,&nbsp;Aleksandra Pejčić,&nbsp;Anita Đurđević-Svraka,&nbsp;Dragan Svraka,&nbsp;Darija Knežević,&nbsp;Tatjana Milivojac,&nbsp;Nataša Bogavac-Stanojević","doi":"10.5937/jomb0-40641","DOIUrl":"10.5937/jomb0-40641","url":null,"abstract":"<p><strong>Background: </strong>The pandemic of severe acute respiratory syndrome by coronavirus 2 (SARS-CoV-2) is a multi-system disease caused by a diffuse systemic process involving a complex interaction of the inflammatory, immunological and coagulative cascades. This study aims to identify the most effective biomarkers to predict poor outcome in intensive care unit (ICU) patients with severe COVID-19 disease.</p><p><strong>Methods: </strong>A single-centre retrospective observational study enrolled 69 deceased and 20 recovered patients treated in the ICU of the General Hospital Gradiska in the period from March 1, 2021. until April 1, 2022. We evaluated the leukocytes (WBC), lymphocytes (LYM), neutrophils (NEU), platelets (PLT), haemoglobin, neutrophil-lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and systemic immune-inflammation index (SII). In addition, we evaluated the IL-6, ferritin, CRP, D-dimer, magnesium, bilirubin and lactate dehydrogenase.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"513-523"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10545360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41133447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vitamin D deficiency in relation with the systemic and central inflammation during multiple sclerosis. 多发性硬化症期间维生素D缺乏和全身和中枢炎症的关系。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-37676
Sawsan Feki, Manel Naifar, Mariem Dammak, Sabrina Majdoub, Salma Sakka, Ali Yesmine Ben, Hend Hachicha, Chokri Mhiri, Fatma Ayadi, Hatem Masmoudi

Background: During the last decade, vitamin D (VitD) has become a topic of interest in immune regulation, especially in multiple sclerosis (MS) disease. Amongst the wide range of effects reported for this vitamin on the immune system, a regulatory role on cytokines production has been described. Our aim is to analyze the status of VitD and its correlation with the circulating inflammation and the intrathecal humoral response during MS.

Methods: We analyzed samples of 318 individuals: 108 MS patients and 210 controls. Determination of 25-(OH) VitD3 level in serum was made using electrochemiluminescence method. Circulating inflammatory cytokines (IL-6, IL-8, IL-10, TNF-a, IL12p70 and IL-1b) were investigated using Cytometer Bead Array Technology. The central humoral response was characterized using CSF isofocusing test and IgG Index calculation.

Results: As expected, mean value of VitD was significantly lower in MS group (26 nmol/L) than in control group (34.75 nmol/L) (p=0.002), with a severe deficiency in 67% of MS patients. Mean value of VitD was significantly lower in MS female patients. Regarding cytokines, mean value of TNFa was significantly higher in MS patients with oligoclonal bands of IgG in the CSF. IL6 was positively correlated with IgG level in serum of MS patients.

Conclusions: Our results support the association of VitD deficiency with MS, especially in female patients of our region. However, the vitamin level seems to not correlate with inflammatory cytokines nor with disability. Interestingly, TNFa and IL6 levels were correlated with the intrathecal synthesis of IgG and the circulating IgG level, respectively.

背景:在过去的十年里,维生素D(VitD)已经成为免疫调节的一个有趣的话题,尤其是在多发性硬化症(MS)疾病中。在报道的这种维生素对免疫系统的广泛影响中,已经描述了其对细胞因子产生的调节作用。我们的目的是分析VitD的状态及其与MS期间循环炎症和鞘内体液反应的相关性。方法:我们分析了318名个体的样本:108名MS患者和210名对照。采用电化学发光法测定血清中25-(OH)VitD3的含量。使用细胞仪珠阵列技术研究循环炎症细胞因子(IL-6、IL-8、IL-10、TNF-a、IL12p70和IL-1b)。用CSF等聚焦试验和IgG指数计算来表征中枢体液反应。结果:不出所料,MS组的VitD平均值(26nmol/L)显著低于对照组(34.75nmol/L,p=0.002),67%的MS患者严重缺乏VitD。多发性硬化症女性患者的VitD平均值显著降低。关于细胞因子,在CSF中IgG寡克隆带的MS患者中,TNFa的平均值显著较高。IL6与MS患者血清IgG水平呈正相关。结论:我们的研究结果支持维生素D缺乏症与多发性硬化症的相关性,尤其是在我们地区的女性患者中。然而,维生素水平似乎与炎症细胞因子无关,也与残疾无关。有趣的是,TNFa和IL6水平分别与鞘内IgG合成和循环IgG水平相关。
{"title":"Vitamin D deficiency in relation with the systemic and central inflammation during multiple sclerosis.","authors":"Sawsan Feki,&nbsp;Manel Naifar,&nbsp;Mariem Dammak,&nbsp;Sabrina Majdoub,&nbsp;Salma Sakka,&nbsp;Ali Yesmine Ben,&nbsp;Hend Hachicha,&nbsp;Chokri Mhiri,&nbsp;Fatma Ayadi,&nbsp;Hatem Masmoudi","doi":"10.5937/jomb0-37676","DOIUrl":"10.5937/jomb0-37676","url":null,"abstract":"<p><strong>Background: </strong>During the last decade, vitamin D (VitD) has become a topic of interest in immune regulation, especially in multiple sclerosis (MS) disease. Amongst the wide range of effects reported for this vitamin on the immune system, a regulatory role on cytokines production has been described. Our aim is to analyze the status of VitD and its correlation with the circulating inflammation and the intrathecal humoral response during MS.</p><p><strong>Methods: </strong>We analyzed samples of 318 individuals: 108 MS patients and 210 controls. Determination of 25-(OH) VitD3 level in serum was made using electrochemiluminescence method. Circulating inflammatory cytokines (IL-6, IL-8, IL-10, TNF-a, IL12p70 and IL-1b) were investigated using Cytometer Bead Array Technology. The central humoral response was characterized using CSF isofocusing test and IgG Index calculation.</p><p><strong>Results: </strong>As expected, mean value of VitD was significantly lower in MS group (26 nmol/L) than in control group (34.75 nmol/L) (p=0.002), with a severe deficiency in 67% of MS patients. Mean value of VitD was significantly lower in MS female patients. Regarding cytokines, mean value of TNFa was significantly higher in MS patients with oligoclonal bands of IgG in the CSF. IL6 was positively correlated with IgG level in serum of MS patients.</p><p><strong>Conclusions: </strong>Our results support the association of VitD deficiency with MS, especially in female patients of our region. However, the vitamin level seems to not correlate with inflammatory cytokines nor with disability. Interestingly, TNFa and IL6 levels were correlated with the intrathecal synthesis of IgG and the circulating IgG level, respectively.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"364-375"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Principal component analysis of the oxidative stress, inflammation, and dyslipidemia influence in patients with different levels of glucoregulation. 氧化应激、炎症和血脂异常对不同血糖调节水平患者影响的主成分分析。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-39636
Maja Malenica, Aleksandra Klisić, Neven Meseldžić, Tanja Dujić, Tamer Bego, Jelena Kotur-Stevuljević

Background: The aim of the study was to explore the mutual relationship between oxidative stress, inflammation and metabolic biomarkers in subjects with prediabetes (PRE), newly diagnosed type 2 diabetes patients (NT2D) and overt type 2 diabetes (T2D) using principal component analysis (PCA) as a thorough statistical approach.

Methods: Glycated hemoglobin, lipid parameters, inflammation (IL-6, CRP and fibrinogen) and oxidative stress markers pro-oxidants (AOPP, PAB, TOS) and antioxidants (PON1, tSHG, TAS) were measured. PCA was applied to explore the factors that the most strongly influenced glucoregulation.

Results: A total of 278 subjects were (i.e., 37 PRE, 42 NT2D and 99 T2D) were compared with 100 healthy subjects as a control group (CG). PCA emphasized 4 different factors explaining 49% of the variance of the tested parameters: oxidative stress-dyslipidemia related factor (with positive loading of TG and tSHG, and with negative loading of HDL-c and TAS), dyslipidaemia related factor (i.e., total cholesterol and LDL-c, both with positive loading), Anthropometric related factor (i.e., waist and hip circumference, both with positive loading) and oxidative stressInflammation related factor (i.e., PAB, fibrinogen, and CRP all with positive loading). Out of these 4 factors, only oxidative stress - dyslipidaemia related factor showed a significant predictive capability towards poor glucoregulation. An increase in this factor by one unit showed a 1.6 times higher probability for poor glucoregulation.

Conclusions: Redox imbalance (determined with lower TAS and higher tSHG), in addition to higher TG and lower HDLc was associated with poor glucoregulation.

背景:本研究的目的是使用主成分分析(PCA)作为一种全面的统计方法,探讨糖尿病前期(PRE)、新诊断的2型糖尿病患者(NT2D)和显性2型糖尿病(T2D)受试者的氧化应激、炎症和代谢生物标志物之间的相互关系。方法:测定糖化血红蛋白、脂质参数、炎症(IL-6、CRP和纤维蛋白原)、氧化应激标志物促氧化剂(AOPP、PAB、TOS)和抗氧化剂(PON1、tSHG、TAS)。应用主成分分析法来探讨对血糖调节影响最大的因素。结果:将278名受试者(即37名PRE、42名NT2D和99名T2D)与100名健康受试者作为对照组(CG)进行比较。PCA强调了4个不同的因素,解释了49%的测试参数方差:氧化应激性血脂异常相关因素(TG和tSHG正负荷,HDL-c和TAS负负荷)、血脂异常相关因子(即总胆固醇和LDL-c,均为正负荷),人体测量相关因素(即腰围和臀围,均为正负荷)和氧化应激炎症相关因素(如PAB、纤维蛋白原和CRP,均为阳性负荷)。在这4个因素中,只有氧化应激-血脂异常相关因素对血糖调节不良具有显著的预测能力。该因子增加一个单位,显示血糖调节不良的概率高1.6倍。结论:氧化还原失衡(由较低的TAS和较高的tSHG确定),除了较高的TG和较低的HDLc外,还与较差的血糖调节有关。
{"title":"Principal component analysis of the oxidative stress, inflammation, and dyslipidemia influence in patients with different levels of glucoregulation.","authors":"Maja Malenica,&nbsp;Aleksandra Klisić,&nbsp;Neven Meseldžić,&nbsp;Tanja Dujić,&nbsp;Tamer Bego,&nbsp;Jelena Kotur-Stevuljević","doi":"10.5937/jomb0-39636","DOIUrl":"10.5937/jomb0-39636","url":null,"abstract":"<p><strong>Background: </strong>The aim of the study was to explore the mutual relationship between oxidative stress, inflammation and metabolic biomarkers in subjects with prediabetes (PRE), newly diagnosed type 2 diabetes patients (NT2D) and overt type 2 diabetes (T2D) using principal component analysis (PCA) as a thorough statistical approach.</p><p><strong>Methods: </strong>Glycated hemoglobin, lipid parameters, inflammation (IL-6, CRP and fibrinogen) and oxidative stress markers pro-oxidants (AOPP, PAB, TOS) and antioxidants (PON1, tSHG, TAS) were measured. PCA was applied to explore the factors that the most strongly influenced glucoregulation.</p><p><strong>Results: </strong>A total of 278 subjects were (i.e., 37 PRE, 42 NT2D and 99 T2D) were compared with 100 healthy subjects as a control group (CG). PCA emphasized 4 different factors explaining 49% of the variance of the tested parameters: oxidative stress-dyslipidemia related factor (with positive loading of TG and tSHG, and with negative loading of HDL-c and TAS), dyslipidaemia related factor (i.e., total cholesterol and LDL-c, both with positive loading), Anthropometric related factor (i.e., waist and hip circumference, both with positive loading) and oxidative stressInflammation related factor (i.e., PAB, fibrinogen, and CRP all with positive loading). Out of these 4 factors, only oxidative stress - dyslipidaemia related factor showed a significant predictive capability towards poor glucoregulation. An increase in this factor by one unit showed a 1.6 times higher probability for poor glucoregulation.</p><p><strong>Conclusions: </strong>Redox imbalance (determined with lower TAS and higher tSHG), in addition to higher TG and lower HDLc was associated with poor glucoregulation.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"427-436"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Spexin level in acute myocardial infarction in the emergency department. Spexin水平在急诊科急性心肌梗死中的应用。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-39485
Yahya Çiftçi, Mehtap Gurger, Evrim Gul, Mustafa Yilmaz, Selda Telo, Metin Atescelik, Goktekin Mehmet Cagri, Kobat Mehmet Ali

Background: We aimed to determine the serum spexin level in patients with acute myocardial infarction (AMI) admitted to the emergency department.

Methods: A total of 100 patients with AMI (50 with ST-segment elevation myocardial infarction (STEMI) and 50 with non-ST-segment elevation myocardial infarction (NSTEMI)) and 50 control group patients with non-cardiac chest pain were included in the study. A detailed anamnesis was taken, a physical examination was performed, and 12-lead electrocardiograms and venous blood samples were taken at the time of admission. Spexin levels were measured via enzyme-linked immunosorbent assay.

Results: Serum spexin levels were significantly lower in the AMI group than in the non-cardiac chest pain group (p<0.001). There was no significant difference in serum spexin levels between STEMI and NSTEMI patients (p=0.83). In receiver operating curve analysis, we detected 58% sensitivity, 76% specificity, 82.9% positive predictive value, and 47.5% negative predictive value with an optimal cutoff value of 532 pg/mL for the diagnosis of AMI.

Conclusions: In this study, serum spexin levels were significantly lower in AMI patients compared to patients with non-cardiac chest pain. The decrease in spexin levels suggests that it has the potential to be used as a diagnostic marker in AMI patients.

背景:我们旨在测定急诊科急性心肌梗死(AMI)患者的血清spexin水平。方法:共纳入100例AMI患者(50例ST段抬高型心肌梗死(STEMI),50例非ST段抬高性心肌梗死(NSTEMI))和50例非心脏性胸痛对照组患者。入院时进行了详细的回顾,进行了身体检查,并采集了12导联心电图和静脉血样。通过酶联免疫吸附测定法测定Spexin水平。结果:AMI组血清spexin水平显著低于非心脏性胸痛组(P结论:在本研究中,AMI患者血清spexin水平显著低于无心脏性胸痛患者。
{"title":"Spexin level in acute myocardial infarction in the emergency department.","authors":"Yahya Çiftçi,&nbsp;Mehtap Gurger,&nbsp;Evrim Gul,&nbsp;Mustafa Yilmaz,&nbsp;Selda Telo,&nbsp;Metin Atescelik,&nbsp;Goktekin Mehmet Cagri,&nbsp;Kobat Mehmet Ali","doi":"10.5937/jomb0-39485","DOIUrl":"10.5937/jomb0-39485","url":null,"abstract":"<p><strong>Background: </strong>We aimed to determine the serum spexin level in patients with acute myocardial infarction (AMI) admitted to the emergency department.</p><p><strong>Methods: </strong>A total of 100 patients with AMI (50 with ST-segment elevation myocardial infarction (STEMI) and 50 with non-ST-segment elevation myocardial infarction (NSTEMI)) and 50 control group patients with non-cardiac chest pain were included in the study. A detailed anamnesis was taken, a physical examination was performed, and 12-lead electrocardiograms and venous blood samples were taken at the time of admission. Spexin levels were measured via enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>Serum spexin levels were significantly lower in the AMI group than in the non-cardiac chest pain group (p<0.001). There was no significant difference in serum spexin levels between STEMI and NSTEMI patients (p=0.83). In receiver operating curve analysis, we detected 58% sensitivity, 76% specificity, 82.9% positive predictive value, and 47.5% negative predictive value with an optimal cutoff value of 532 pg/mL for the diagnosis of AMI.</p><p><strong>Conclusions: </strong>In this study, serum spexin levels were significantly lower in AMI patients compared to patients with non-cardiac chest pain. The decrease in spexin levels suggests that it has the potential to be used as a diagnostic marker in AMI patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"407-411"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of Interleukin-17, Interleukin-23, neopterin and Nesfatin-1 levels in the sera of Hashimoto patients. 桥本患者血清中白细胞介素-17、白细胞介蛋白-23、新蝶呤和内脂蛋白-1水平的分析。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-40683
Nihayet Bayraktar, Mehmet Ali Eren, Mustafa Bayraktar, Ali Öztürk, Hamza Erdoğdu

Background: Hashimoto's thyroiditis (HT) is an autoimmune disorder affecting the thyroid gland and may present as goiter or atrophic thyroiditis that may result in various metabolic and inflammatory disorders. The aim of this study is to determine the changes in serum levels of interleukin-17 (IL-17), IL-23, neopterin, and nesfatin-1 parameters in HT patients and to evaluate the possible relationship among these parameters.

Methods: 90 HT patients and 30 healthy individuals were included in this study. Demographic data of the patients included in the study were recorded and detailed physical examinations were performed. IL-17, IL-23, neopterin, and nesfatin-1 levels were measured in the serum samples of the participants by the ELISA method.

背景:桥本甲状腺炎(HT)是一种影响甲状腺的自身免疫性疾病,可能表现为甲状腺肿或萎缩性甲状腺炎,可能导致各种代谢和炎症障碍。本研究的目的是确定HT患者血清白细胞介素17(IL-17)、IL-23、新蝶呤和内脂蛋白-1参数水平的变化,并评估这些参数之间的可能关系。方法:90例HT患者和30例健康人参加本研究。记录研究中患者的人口学数据,并进行详细的身体检查。通过ELISA方法测量参与者血清样本中的IL-17、IL-23、新蝶呤和内脂蛋白-1水平。
{"title":"Analysis of Interleukin-17, Interleukin-23, neopterin and Nesfatin-1 levels in the sera of Hashimoto patients.","authors":"Nihayet Bayraktar,&nbsp;Mehmet Ali Eren,&nbsp;Mustafa Bayraktar,&nbsp;Ali Öztürk,&nbsp;Hamza Erdoğdu","doi":"10.5937/jomb0-40683","DOIUrl":"10.5937/jomb0-40683","url":null,"abstract":"<p><strong>Background: </strong>Hashimoto's thyroiditis (HT) is an autoimmune disorder affecting the thyroid gland and may present as goiter or atrophic thyroiditis that may result in various metabolic and inflammatory disorders. The aim of this study is to determine the changes in serum levels of interleukin-17 (IL-17), IL-23, neopterin, and nesfatin-1 parameters in HT patients and to evaluate the possible relationship among these parameters.</p><p><strong>Methods: </strong>90 HT patients and 30 healthy individuals were included in this study. Demographic data of the patients included in the study were recorded and detailed physical examinations were performed. IL-17, IL-23, neopterin, and nesfatin-1 levels were measured in the serum samples of the participants by the ELISA method.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"460-468"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41116827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of serum vitamin B12 levels in patients with COVID-19 infection: A case-control study. 新冠肺炎感染患者血清维生素B12水平的评估:一项病例对照研究。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-25 DOI: 10.5937/jomb0-42357
Yılmaz Sezgin

Background: COVID-19 disease affects the respiratory and cardiovascular systems. Vitamin B12 has been associated with A1AT, one of the protective factors of lung tissue, and homocysteine among the cardiovascular risk factors. Therefore we suggest that low vitamin B12 levels are associated with a disposition to COVID-19 infection. This study aims to determine whether there is a relationship between COVID-19 infection and serum vitamin B12 levels.

Methods: This research is a case-control study. Seventy-six people with COVID-19 constituted the case group. Seventy-six people without COVID-19 formed the control group. Vitamin B12 and homocysteine levels of 152 patients included in the study were analyzed.

Results: The odds ratio for vitamin B12 was 0.99 (0.978-0.995). When the value of the vitamin B12 variable decreases by one unit, the risk of COVID-19 increases by 1%. The odds ratio for homocysteine was 1.81 (1.414-2.325). When the value of the homocysteine variable increases by one unit, the risk of COVID-19 increases by 1.81 times. According to ROC analysis, when serum vitamin B12 is below 222.5 ng/L and homocysteine is above 13.7 mmol/L, it may increase the risk of COVID-19.

Conclusions: We suggest that patients with low vitamin B12 levels and high homocysteine levels are more severely affected by COVID-19 infection.

背景:新冠肺炎疾病影响呼吸系统和心血管系统。维生素B12与肺组织的保护因子之一A1AT和心血管危险因素中的同型半胱氨酸有关。因此,我们认为维生素B12水平低与新冠肺炎感染倾向有关。本研究旨在确定新冠肺炎感染与血清维生素B12水平之间是否存在关系。方法:本研究为病例对照研究。76名新冠肺炎患者构成病例组。76名无新冠肺炎的患者组成对照组。对纳入研究的152名患者的维生素B12和同型半胱氨酸水平进行了分析。结果:维生素B12的比值比为0.99(0.978-0.995)。当维生素B12变量的值降低一个单位时,患新冠肺炎的风险增加1%。同型半胱氨酸的比值比为1.81(1.414-2.325)。当同型半胱氨酸变量的值增加一个单位时,新冠肺炎的风险增加1.81倍。根据ROC分析,当血清维生素B12低于222.5纳克/升,同型半胱氨酸高于13.7毫摩尔/升时,可能会增加患新冠肺炎的风险。结论:我们认为,维生素B12水平低、同型半胱氨酸水平高的患者受新冠肺炎感染的影响更严重。
{"title":"Evaluation of serum vitamin B12 levels in patients with COVID-19 infection: A case-control study.","authors":"Yılmaz Sezgin","doi":"10.5937/jomb0-42357","DOIUrl":"https://doi.org/10.5937/jomb0-42357","url":null,"abstract":"<p><strong>Background: </strong>COVID-19 disease affects the respiratory and cardiovascular systems. Vitamin B12 has been associated with A1AT, one of the protective factors of lung tissue, and homocysteine among the cardiovascular risk factors. Therefore we suggest that low vitamin B12 levels are associated with a disposition to COVID-19 infection. This study aims to determine whether there is a relationship between COVID-19 infection and serum vitamin B12 levels.</p><p><strong>Methods: </strong>This research is a case-control study. Seventy-six people with COVID-19 constituted the case group. Seventy-six people without COVID-19 formed the control group. Vitamin B12 and homocysteine levels of 152 patients included in the study were analyzed.</p><p><strong>Results: </strong>The odds ratio for vitamin B12 was 0.99 (0.978-0.995). When the value of the vitamin B12 variable decreases by one unit, the risk of COVID-19 increases by 1%. The odds ratio for homocysteine was 1.81 (1.414-2.325). When the value of the homocysteine variable increases by one unit, the risk of COVID-19 increases by 1.81 times. According to ROC analysis, when serum vitamin B12 is below 222.5 ng/L and homocysteine is above 13.7 mmol/L, it may increase the risk of COVID-19.</p><p><strong>Conclusions: </strong>We suggest that patients with low vitamin B12 levels and high homocysteine levels are more severely affected by COVID-19 infection.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 3","pages":"524-529"},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10543489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
Journal of Medical Biochemistry
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1