首页 > 最新文献

Journal of Neural Transmission - Parkinson's Disease and Dementia Section最新文献

英文 中文
Are NMDA antagonistic properties relevant for antiparkinsonian-like activity in rats?--case of amantadine and memantine. NMDA的拮抗特性是否与大鼠的抗帕金森样活性相关?金刚烷胺和美金刚胺的案例。
W Danysz, M Gossel, W Zajaczkowski, D Dill, G Quack

Amantadine (25, 50, 100 mg/kg), memantine (5, 10, 20 mg/kg) and MK-801 (0.05, 0.1, 0.2 mg/kg), all having NMDA channel blocking properties, were compared in three tests used for screening of antiparkinsonian agents in rats, namely: haloperidol-induced catalepsy, locomotor activity in monoamine depleted rats and rotation in rats with a unilateral substantia nigra lesion. Additionally, plasma levels of amantadine and memantine were assessed to gain an insight into the concentration ranges achieved at behaviorally active doses. Amantadine and (+)-MK-801 produced dose-dependent inhibition of haloperidol-induced catalepsy while memantine was less efficacious producing clear-cut anticataleptic action at a dose of 10 mg/kg only but failing at 20 mg/kg due to myorelaxant activity. All agents attenuated sedation in monoamine depleted rats with amantadine being the least and MK-801 being the most effective. The same rank order of efficacy was seen in inducing ipsilateral rotations in rats after a substantia nigra lesion. On the basis of the present study and published data, it can be assumed that the doses of amantadine, memantine and MK-801 showing antiparkinsonian-like activity in animals result in plasma levels leading to NMDA antagonism. However, in the haloperidol-induced catalepsy test the efficacy of amantadine was higher than memantine, while the opposite was true for rotation and reserpine-induced sedation indicating pharmacodynamic differences between both agents.

对具有NMDA通道阻断特性的金刚烷胺(25、50、100 mg/kg)、美金刚胺(5、10、20 mg/kg)和MK-801(0.05、0.1、0.2 mg/kg)进行了氟哌啶醇诱导的猝倒、单胺缺失大鼠的运动活性和单侧黑质病变大鼠的旋转三种试验,以筛选大鼠抗帕金森药物。此外,评估了金刚烷胺和美金刚的血浆水平,以深入了解在行为活性剂量下达到的浓度范围。金刚烷胺和(+)-MK-801对氟哌啶醇诱导的猝厥产生剂量依赖的抑制作用,而美金刚仅在10 mg/kg剂量时产生明确的抗猝厥作用效果较差,但在20 mg/kg剂量时由于肌肉松弛活性而失效。所有药物对单胺衰竭大鼠的镇静作用均有减弱作用,金刚烷胺作用最小,MK-801最有效。在大鼠黑质损伤后诱导同侧旋转的效果也相同。根据目前的研究和已发表的数据,可以假设金刚烷胺、美金刚和MK-801在动物中显示抗帕金森样活性的剂量导致血浆水平导致NMDA拮抗。然而,在氟哌啶醇诱导的镇静试验中,金刚烷胺的效果高于美金刚,而旋转和利血平诱导的镇静效果则相反,这表明两种药物之间存在药效学差异。
{"title":"Are NMDA antagonistic properties relevant for antiparkinsonian-like activity in rats?--case of amantadine and memantine.","authors":"W Danysz,&nbsp;M Gossel,&nbsp;W Zajaczkowski,&nbsp;D Dill,&nbsp;G Quack","doi":"10.1007/BF02253435","DOIUrl":"https://doi.org/10.1007/BF02253435","url":null,"abstract":"<p><p>Amantadine (25, 50, 100 mg/kg), memantine (5, 10, 20 mg/kg) and MK-801 (0.05, 0.1, 0.2 mg/kg), all having NMDA channel blocking properties, were compared in three tests used for screening of antiparkinsonian agents in rats, namely: haloperidol-induced catalepsy, locomotor activity in monoamine depleted rats and rotation in rats with a unilateral substantia nigra lesion. Additionally, plasma levels of amantadine and memantine were assessed to gain an insight into the concentration ranges achieved at behaviorally active doses. Amantadine and (+)-MK-801 produced dose-dependent inhibition of haloperidol-induced catalepsy while memantine was less efficacious producing clear-cut anticataleptic action at a dose of 10 mg/kg only but failing at 20 mg/kg due to myorelaxant activity. All agents attenuated sedation in monoamine depleted rats with amantadine being the least and MK-801 being the most effective. The same rank order of efficacy was seen in inducing ipsilateral rotations in rats after a substantia nigra lesion. On the basis of the present study and published data, it can be assumed that the doses of amantadine, memantine and MK-801 showing antiparkinsonian-like activity in animals result in plasma levels leading to NMDA antagonism. However, in the haloperidol-induced catalepsy test the efficacy of amantadine was higher than memantine, while the opposite was true for rotation and reserpine-induced sedation indicating pharmacodynamic differences between both agents.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"7 3","pages":"155-66"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02253435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18714052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 71
Pyritinol facilitates the recovery of cortical cholinergic deficits caused by nucleus basalis lesions. 吡啶醇促进基底核损伤引起的皮质胆碱能缺陷的恢复。
A Toledano, M L Bentura

The effect of a nootropic, Pyritinol, on the recovery of cortical cholinergic deficits induced by injury of the nucleus basalis has been tested on two groups of unilateral quisqualic acid nbM-lesioned rats. The first group had a 30 nmol lesion producing a cortical cholinergic impairment at 21 days, with a spontaneous recovery at 45 days. The second group had a 50 nmol lesion that produced a deeper cholinergic deficit, which did not recover at 45 days. Pyritinol enhanced the recovery in the 30 nmol group of animals on the 21st day after surgery. The recovery was measured as an increase in the activities of acetylcholinesterase (AChE), choline acetyltransferase (ChAT) and the high affinity choline uptake system, and the histochemical densities of the cortical AChE network and the M2 receptor. Histochemical analysis of the nbM enabled cortical recovery to be related to the number of surviving neurons and also to their hypertrophy and AChE-ChAT hyperactivity. Pyritinol enhanced recovery in 30 nmol lesioned animals but in the other group, with a lower number of surviving neurons and a lower ability of the cells to become hypertrophic, the drug was unable to promote cortical recovery.

在两组单侧准质酸nbm损伤大鼠身上,研究了益智药吡啶醇对基底核损伤引起的皮质胆碱能缺损的恢复作用。第一组有30 nmol的损伤,在21天产生皮质胆碱能损伤,在45天自然恢复。第二组有50 nmol的损伤,产生更深的胆碱能缺陷,在45天没有恢复。30 nmol吡啶醇组动物术后第21天恢复较好。恢复测量为乙酰胆碱酯酶(AChE)、胆碱乙酰转移酶(ChAT)和高亲和力胆碱摄取系统活性的增加,以及皮质AChE网络和M2受体的组织化学密度的增加。nbM的组织化学分析表明,皮层恢复与存活神经元的数量有关,也与它们的肥大和AChE-ChAT多动有关。Pyritinol促进了30只nmol损伤动物的恢复,但在另一组中,由于存活神经元数量较少,细胞变得肥大的能力较低,该药物无法促进皮质恢复。
{"title":"Pyritinol facilitates the recovery of cortical cholinergic deficits caused by nucleus basalis lesions.","authors":"A Toledano,&nbsp;M L Bentura","doi":"10.1007/BF02253438","DOIUrl":"https://doi.org/10.1007/BF02253438","url":null,"abstract":"<p><p>The effect of a nootropic, Pyritinol, on the recovery of cortical cholinergic deficits induced by injury of the nucleus basalis has been tested on two groups of unilateral quisqualic acid nbM-lesioned rats. The first group had a 30 nmol lesion producing a cortical cholinergic impairment at 21 days, with a spontaneous recovery at 45 days. The second group had a 50 nmol lesion that produced a deeper cholinergic deficit, which did not recover at 45 days. Pyritinol enhanced the recovery in the 30 nmol group of animals on the 21st day after surgery. The recovery was measured as an increase in the activities of acetylcholinesterase (AChE), choline acetyltransferase (ChAT) and the high affinity choline uptake system, and the histochemical densities of the cortical AChE network and the M2 receptor. Histochemical analysis of the nbM enabled cortical recovery to be related to the number of surviving neurons and also to their hypertrophy and AChE-ChAT hyperactivity. Pyritinol enhanced recovery in 30 nmol lesioned animals but in the other group, with a lower number of surviving neurons and a lower ability of the cells to become hypertrophic, the drug was unable to promote cortical recovery.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"7 3","pages":"195-209"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02253438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18714744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Intranigral injected iron progressively reduces striatal dopamine metabolism. 黑质内注射铁逐渐减少纹状体多巴胺代谢。
W Wesemann, S Blaschke, M Solbach, C Grote, H W Clement, P Riederer

The striatal dopamine metabolism of the rat was followed 1, 3, and 6 weeks after unilateral intranigral iron (III) (50- and 1.5 micrograms) application. For both concentrations a progredient decrease of extraneuronal 3.4-dihydroxyphenylacetic acid (DOPA) levels was observed in the ipsilateral striatum.

在单侧神经内铁(III)(50和1.5微克)应用后1、3和6周,观察大鼠纹状体多巴胺代谢。两种浓度下,同侧纹状体神经元外3.4-二羟基苯乙酸(DOPA)水平均逐渐下降。
{"title":"Intranigral injected iron progressively reduces striatal dopamine metabolism.","authors":"W Wesemann,&nbsp;S Blaschke,&nbsp;M Solbach,&nbsp;C Grote,&nbsp;H W Clement,&nbsp;P Riederer","doi":"10.1007/BF02260941","DOIUrl":"https://doi.org/10.1007/BF02260941","url":null,"abstract":"<p><p>The striatal dopamine metabolism of the rat was followed 1, 3, and 6 weeks after unilateral intranigral iron (III) (50- and 1.5 micrograms) application. For both concentrations a progredient decrease of extraneuronal 3.4-dihydroxyphenylacetic acid (DOPA) levels was observed in the ipsilateral striatum.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"8 3","pages":"209-14"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02260941","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18749738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 38
Reference memory is affected by transient bilateral clamping of the carotid arteries in rats (BCCA). 暂时性双侧颈动脉夹持对大鼠参考记忆的影响。
C Heim, K H Sontag

Rats were subjected to 60 min of bilateral clamping of the carotid arteries (BCCA) in pentobarbital anaesthesia and tested in a hole board with 8 of the 25 holes baited with food pellets hidden in a serial order. All rats learned to recognize the pattern as a reference during an acquisition period at 2-14 days post surgery, i.e. reference memory. During the recall tests 29-37, 165-175 and 240-250 days later BCCA animals showed a decrease in memory revealing a significant increase in reference memory errors. The number of working memory errors, i.e. reexamination of inspected previously baited holes, did not differ from those of sham operated controls. The number of pellets eaten in serial order from hole 1 to hole 8 was significantly decreased in BCCA animals compared with controls, while the number of holes inspected by the animals in the two groups were the same. Thus the observed behaviour changes appear not to be caused by motor or motivation deficiencies.

大鼠在戊巴比妥麻醉下双侧夹持颈动脉(BCCA) 60分钟,并在孔板中进行测试,25个孔中有8个孔按顺序隐藏诱饵食物颗粒。在术后2-14天的习得期,所有大鼠都学会了将模式识别为参考,即参考记忆。在29-37、165-175和240-250天后的回忆测试中,BCCA动物的记忆力下降,而参考记忆错误显著增加。工作记忆错误的数量,即重新检查先前检查过的诱饵孔,与假手术对照组没有区别。与对照组相比,BCCA组动物从1号孔到8号孔依次进食的颗粒数量显著减少,而两组动物检查的孔数相同。因此,观察到的行为变化似乎不是由运动或动机缺陷引起的。
{"title":"Reference memory is affected by transient bilateral clamping of the carotid arteries in rats (BCCA).","authors":"C Heim,&nbsp;K H Sontag","doi":"10.1007/BF02252662","DOIUrl":"https://doi.org/10.1007/BF02252662","url":null,"abstract":"<p><p>Rats were subjected to 60 min of bilateral clamping of the carotid arteries (BCCA) in pentobarbital anaesthesia and tested in a hole board with 8 of the 25 holes baited with food pellets hidden in a serial order. All rats learned to recognize the pattern as a reference during an acquisition period at 2-14 days post surgery, i.e. reference memory. During the recall tests 29-37, 165-175 and 240-250 days later BCCA animals showed a decrease in memory revealing a significant increase in reference memory errors. The number of working memory errors, i.e. reexamination of inspected previously baited holes, did not differ from those of sham operated controls. The number of pellets eaten in serial order from hole 1 to hole 8 was significantly decreased in BCCA animals compared with controls, while the number of holes inspected by the animals in the two groups were the same. Thus the observed behaviour changes appear not to be caused by motor or motivation deficiencies.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"7 1","pages":"47-59"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02252662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19560262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The clinimetrics of hypokinesia in Parkinson's disease: subjective versus objective assessment. 帕金森病运动不足的临床指标:主观与客观评估。
J J van Hilten, J I Hoff, H A Middelkoop, R A Roos

In this study we evaluate the feasibility of measures that reflect different characteristics of motor activity and immobility in the objective quantification of hypokinesia. Because by definition hypokinesia can only be assessed over a period of time, continuous activity monitoring was used during 5 successive days in the home setting in 64 patients with Parkinson's disease (PD) and 104 healthy elderly subjects. In the patients we also evaluated the relation between the monitor measures and subjective measures of hypokinesia and age. Compared to the healthy elderly subjects, PD patients have a decreased activity level, increased proportion of time without movement, elevated mean duration of immobility, and decreased percentage of short-lasting immobility periods. Differences between both groups were most prominent for those measures that incorporate or reflect immobility. Moreover, in the PD patients the mean duration of immobility and percentage of short-lasting immobility periods show an apparent lack of relation with age and clinical ratings obtained from the UPDRS. In conclusion, our findings underscore the poor representation of hypokinesia in the UPDRS and value of objective quantification of this fundamental impairment of PD.

在这项研究中,我们评估了反映运动活动和不动的不同特征的措施在客观量化运动不足的可行性。由于根据定义,运动不足只能在一段时间内进行评估,因此我们在家中对64名帕金森病患者和104名健康老年人进行了连续5天的活动监测。在患者中,我们还评估了运动不足的监测指标和主观指标与年龄的关系。与健康老年受试者相比,PD患者活动水平降低,不活动时间比例增加,平均不活动时间增加,短时间不活动时间百分比减少。两组之间的差异最突出的是那些纳入或反映不动的措施。此外,在PD患者中,平均不活动时间和短期不活动时间的百分比明显缺乏与年龄和UPDRS获得的临床评分的关系。总之,我们的研究结果强调了运动不足在UPDRS中的代表性不足,以及客观量化PD基本损害的价值。
{"title":"The clinimetrics of hypokinesia in Parkinson's disease: subjective versus objective assessment.","authors":"J J van Hilten,&nbsp;J I Hoff,&nbsp;H A Middelkoop,&nbsp;R A Roos","doi":"10.1007/BF02250922","DOIUrl":"https://doi.org/10.1007/BF02250922","url":null,"abstract":"<p><p>In this study we evaluate the feasibility of measures that reflect different characteristics of motor activity and immobility in the objective quantification of hypokinesia. Because by definition hypokinesia can only be assessed over a period of time, continuous activity monitoring was used during 5 successive days in the home setting in 64 patients with Parkinson's disease (PD) and 104 healthy elderly subjects. In the patients we also evaluated the relation between the monitor measures and subjective measures of hypokinesia and age. Compared to the healthy elderly subjects, PD patients have a decreased activity level, increased proportion of time without movement, elevated mean duration of immobility, and decreased percentage of short-lasting immobility periods. Differences between both groups were most prominent for those measures that incorporate or reflect immobility. Moreover, in the PD patients the mean duration of immobility and percentage of short-lasting immobility periods show an apparent lack of relation with age and clinical ratings obtained from the UPDRS. In conclusion, our findings underscore the poor representation of hypokinesia in the UPDRS and value of objective quantification of this fundamental impairment of PD.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"8 1-2","pages":"117-21"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02250922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18890431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Quantification of mRNA of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in the substantia nigra in Parkinson's disease and schizophrenia. 帕金森病和精神分裂症黑质中酪氨酸羟化酶和芳香l -氨基酸脱羧酶mRNA的定量分析。
H Ichinose, T Ohye, K Fujita, F Pantucek, K Lange, P Riederer, T Nagatsu

Using the reverse transcription-polymerase chain reaction (RT-PCR), we developed a sensitive and quantitative method to detect all four types of human tyrosine hydroxylase (TH) mRNAs in the human brain (substantia nigra). All four types of TH mRNAs were found in the substantia nigra in the control brains examined, and the ratio of type-1, type-2, type-3, and type-4 mRNAs to the total amount of TH was 45, 52, 1.4, and 2.1%, respectively. The average amount of total TH mRNA in the normal brain (substantia nigra) was 5.5 amol of TH mRNA per microgram of total RNA. The ratios of four TH isoforms were not altered significantly in Parkinson's disease or schizophrenia. Further we measured the relative amount of aromatic L-amino acid decarboxylase (AADC) and beta-actin mRNAs in the brain samples. TH and AADC mRNAs were highly correlated in the control cases. We found that parkinsonian brains had very low levels of all four TH isoforms and AADC mRNAs in the substantia nigra compared with control brains, while no significant differences were found between schizophrenic brains and normal ones. Since the decrease in AADC mRNA was comparable to that in TH mRNA, the alteration of TH in Parkinson's disease would not be a primary event, but it would reflect the degeneration of dopaminergic neurons in the substantia nigra. This is the first reported measurement of mRNA contents of TH isoforms and AADC in Parkinson's disease and schizophrenia.

利用逆转录聚合酶链反应(RT-PCR),我们建立了一种灵敏的定量检测人脑(黑质)中所有四种类型的人酪氨酸羟化酶(TH) mrna的方法。在对照组脑黑质中均发现了四种类型的TH mrna,其中1型、2型、3型和4型mrna占TH总量的比例分别为45%、52%、1.4%和2.1%。正常脑(黑质)中总TH mRNA的平均含量为每微克总RNA 5.5 amol。四种TH亚型的比例在帕金森病或精神分裂症中没有显著改变。进一步,我们测量了脑样品中芳香l -氨基酸脱羧酶(AADC)和β -肌动蛋白mrna的相对数量。在对照组中,TH和AADC mrna高度相关。我们发现,与对照组相比,帕金森大脑黑质中所有四种TH亚型和AADC mrna的水平都非常低,而精神分裂症大脑与正常大脑之间没有显著差异。由于AADC mRNA的减少与TH mRNA的减少相当,因此TH在帕金森病中的改变不会是主要事件,但它可能反映了黑质多巴胺能神经元的变性。这是首次报道的测量帕金森病和精神分裂症中TH亚型和AADC mRNA含量的方法。
{"title":"Quantification of mRNA of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in the substantia nigra in Parkinson's disease and schizophrenia.","authors":"H Ichinose,&nbsp;T Ohye,&nbsp;K Fujita,&nbsp;F Pantucek,&nbsp;K Lange,&nbsp;P Riederer,&nbsp;T Nagatsu","doi":"10.1007/BF02250926","DOIUrl":"https://doi.org/10.1007/BF02250926","url":null,"abstract":"<p><p>Using the reverse transcription-polymerase chain reaction (RT-PCR), we developed a sensitive and quantitative method to detect all four types of human tyrosine hydroxylase (TH) mRNAs in the human brain (substantia nigra). All four types of TH mRNAs were found in the substantia nigra in the control brains examined, and the ratio of type-1, type-2, type-3, and type-4 mRNAs to the total amount of TH was 45, 52, 1.4, and 2.1%, respectively. The average amount of total TH mRNA in the normal brain (substantia nigra) was 5.5 amol of TH mRNA per microgram of total RNA. The ratios of four TH isoforms were not altered significantly in Parkinson's disease or schizophrenia. Further we measured the relative amount of aromatic L-amino acid decarboxylase (AADC) and beta-actin mRNAs in the brain samples. TH and AADC mRNAs were highly correlated in the control cases. We found that parkinsonian brains had very low levels of all four TH isoforms and AADC mRNAs in the substantia nigra compared with control brains, while no significant differences were found between schizophrenic brains and normal ones. Since the decrease in AADC mRNA was comparable to that in TH mRNA, the alteration of TH in Parkinson's disease would not be a primary event, but it would reflect the degeneration of dopaminergic neurons in the substantia nigra. This is the first reported measurement of mRNA contents of TH isoforms and AADC in Parkinson's disease and schizophrenia.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"8 1-2","pages":"149-58"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02250926","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18890435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 85
Protective effects of 5-HT1A receptor agonists against neuronal damage demonstrated in vivo and in vitro. 5-HT1A受体激动剂在体内和体外对神经元损伤的保护作用。
B Peruche, C Backhauss, J H Prehn, J Krieglstein

The aim of the present study was to evaluate the neuroprotective effect of the 5-hydroxytryptamine1A (5-HT1A) agonists, CM 57493 and urapidil, in vivo and in vitro, respectively. In vivo permanent occlusion of the middle cerebral artery (MCA) was performed in male Wistar rats. Forty-eight hours after electrocoagulation of the MCA the infarct volume was determined. Pretreatment of the rat with the 5-HT1A agonist urapidil significantly reduced infarct development. The neuroprotective effect of the agent was restricted to the cortical area; the striatal damage was not influenced. As the stimulation of the 5-HT1A receptor by serotonin is supposed to induce inhibitory, hyperpolarizing effects by opening of a Ca(2+)-independent neuronal K+ ionophore, the efficacy of agonistic drugs directly on the neuron was investigated in vitro. Cyanide-induced cytotoxic hypoxia as well as glutamate-induced excitotoxicity were performed using primary neuronal cell cultures from chick embryo cerebral hemispheres. Treatment with the 5-HT1A agonists urapidil and CM 57493 significantly increased protein content of hypoxic cultures. CM 57493 added to the culture medium (1-10 microM) during and up to 24 h after glutamate exposure ameliorated viability of the neurons. The results demonstrate neuroprotective potency of the 5-HT1A agonists, urapidil and CM 57493, when applied under hypoxic, excitotoxic and ischemic conditions in vivo and in vitro, respectively. Both, presynaptically induced inhibition of glutamate release as well as postsynaptically induced inhibition of neuronal excitability could be discussed as possible mechanisms of action of the 5-HT1A receptor agonism.

本研究的目的是评估5-羟色胺1a (5-HT1A)激动剂CM 57493和乌拉地尔在体内和体外的神经保护作用。在体内对雄性Wistar大鼠进行大脑中动脉(MCA)永久性闭塞。电凝后48小时测定心肌梗死体积。用5-HT1A激动剂乌拉地尔预处理大鼠可显著减少梗死的发生。该药的神经保护作用仅限于皮质区;纹状体损伤不受影响。由于血清素刺激5-HT1A受体可能通过打开Ca(2+)不依赖的神经元K+离子载体而诱导抑制、超极化效应,因此我们在体外研究了直接作用于神经元的激动剂药物的疗效。用鸡胚大脑半球原代神经元细胞培养物进行了氰化物诱导的细胞毒性缺氧和谷氨酸诱导的兴奋毒性实验。5-HT1A激动剂乌拉地尔和CM 57493显著增加了缺氧培养物的蛋白质含量。在谷氨酸暴露期间和暴露后24小时,将CM 57493添加到培养基中(1-10微米)可改善神经元的活力。结果表明,5-HT1A激动剂乌拉地尔和CM 57493分别在体内和体外缺氧、兴奋毒性和缺血条件下具有神经保护作用。突触前诱导的谷氨酸释放抑制和突触后诱导的神经元兴奋性抑制都可以作为5-HT1A受体激动作用的可能机制进行讨论。
{"title":"Protective effects of 5-HT1A receptor agonists against neuronal damage demonstrated in vivo and in vitro.","authors":"B Peruche,&nbsp;C Backhauss,&nbsp;J H Prehn,&nbsp;J Krieglstein","doi":"10.1007/BF02250918","DOIUrl":"https://doi.org/10.1007/BF02250918","url":null,"abstract":"<p><p>The aim of the present study was to evaluate the neuroprotective effect of the 5-hydroxytryptamine1A (5-HT1A) agonists, CM 57493 and urapidil, in vivo and in vitro, respectively. In vivo permanent occlusion of the middle cerebral artery (MCA) was performed in male Wistar rats. Forty-eight hours after electrocoagulation of the MCA the infarct volume was determined. Pretreatment of the rat with the 5-HT1A agonist urapidil significantly reduced infarct development. The neuroprotective effect of the agent was restricted to the cortical area; the striatal damage was not influenced. As the stimulation of the 5-HT1A receptor by serotonin is supposed to induce inhibitory, hyperpolarizing effects by opening of a Ca(2+)-independent neuronal K+ ionophore, the efficacy of agonistic drugs directly on the neuron was investigated in vitro. Cyanide-induced cytotoxic hypoxia as well as glutamate-induced excitotoxicity were performed using primary neuronal cell cultures from chick embryo cerebral hemispheres. Treatment with the 5-HT1A agonists urapidil and CM 57493 significantly increased protein content of hypoxic cultures. CM 57493 added to the culture medium (1-10 microM) during and up to 24 h after glutamate exposure ameliorated viability of the neurons. The results demonstrate neuroprotective potency of the 5-HT1A agonists, urapidil and CM 57493, when applied under hypoxic, excitotoxic and ischemic conditions in vivo and in vitro, respectively. Both, presynaptically induced inhibition of glutamate release as well as postsynaptically induced inhibition of neuronal excitability could be discussed as possible mechanisms of action of the 5-HT1A receptor agonism.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"8 1-2","pages":"73-83"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02250918","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18890436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Genetic factors in the etiology of idiopathic Parkinson's disease. 遗传因素在特发性帕金森病病因中的作用。
P Vieregge

Overshadowed by a vigorous search for an environmentally-derived toxin that would be possibly relevant for the pathogenesis of idiopathic Parkinson's disease (PD), genetic factors have largely been neglected for this condition during the last two decades. Recent descriptions of kindreds over three or more generations with several family members affected have renewed the interest in genetics of PD. Concurring with this, diagnostic concepts and pathologic criteria for PD and for idiopathic Lewy-body (LB) disease have been reevaluated such that LB-proven parkinsonism is sufficiently differentiated from familial parkinsonism without LB pathology. Surveys on genetic epidemiology in PD have confirmed the 19th century's notion that 10 to 15% of PD index cases report a further family member with PD. These figures were, however, substantiated on a statistical basis only in single surveys when comparisons were made with the numbers of PD relatives in control index cases. Twin studies did not reveal a higher rate of concordance within monozygotic pairs than in dizygotic pairs. Tests of striatal 18-F-Dopa uptake in clinically unaffected mono- and dizygotic co-twins did not alter the ratio between the concordance rates. Though not excluded by the twin studies, multifactorial (or polygenic) inheritance as well as mitochondrial inheritance are at present less likely to cover most of the inheritance pattern in familial LB parkinsonism. Instead, autosomal dominant inheritance with reduced penetrance is the most probable inheritance pattern for most of the reported pedigrees. Molecular genetic investigations have to consider the biochemical basis of the age- and region-specific pathology of PD. The first analyses of linkage and allelic associations gave inconclusive results in sporadic and familial PD. The hunt for metabolic factors that link geno- and phenotype expression in PD will continue.

在过去的二十年里,由于对一种可能与特发性帕金森病(PD)发病机制有关的环境来源毒素的大力研究,遗传因素在很大程度上被忽视了。最近对三代或三代以上家族成员患病的描述重新引起了人们对帕金森病遗传学的兴趣。与此同时,PD和特发性路易体病(LB)的诊断概念和病理标准已被重新评估,从而使LB证实的帕金森病与没有LB病理的家族性帕金森病充分区分开来。帕金森病的遗传流行病学调查证实了19世纪的观点,即10 - 15%的帕金森病指数病例报告有其他家庭成员患有帕金森病。然而,当与对照指数病例中PD亲属的数量进行比较时,这些数字仅在统计基础上得到证实。双生子研究并没有显示同卵对的一致性比异卵对高。在临床未受影响的单卵双胞胎和异卵双胞胎中,纹状体18- f -多巴摄取试验没有改变一致性率之间的比率。虽然没有被双胞胎研究排除,但多因子(或多基因)遗传以及线粒体遗传目前不太可能涵盖家族性LB帕金森病的大部分遗传模式。相反,外显率降低的常染色体显性遗传是大多数报道的谱系中最可能的遗传模式。分子遗传学研究必须考虑PD的年龄和区域特异性病理的生化基础。对散发性和家族性帕金森病的连锁和等位基因关联的首次分析没有给出确定的结果。寻找PD中与基因和表型表达相关的代谢因子将继续进行。
{"title":"Genetic factors in the etiology of idiopathic Parkinson's disease.","authors":"P Vieregge","doi":"10.1007/BF02250916","DOIUrl":"https://doi.org/10.1007/BF02250916","url":null,"abstract":"<p><p>Overshadowed by a vigorous search for an environmentally-derived toxin that would be possibly relevant for the pathogenesis of idiopathic Parkinson's disease (PD), genetic factors have largely been neglected for this condition during the last two decades. Recent descriptions of kindreds over three or more generations with several family members affected have renewed the interest in genetics of PD. Concurring with this, diagnostic concepts and pathologic criteria for PD and for idiopathic Lewy-body (LB) disease have been reevaluated such that LB-proven parkinsonism is sufficiently differentiated from familial parkinsonism without LB pathology. Surveys on genetic epidemiology in PD have confirmed the 19th century's notion that 10 to 15% of PD index cases report a further family member with PD. These figures were, however, substantiated on a statistical basis only in single surveys when comparisons were made with the numbers of PD relatives in control index cases. Twin studies did not reveal a higher rate of concordance within monozygotic pairs than in dizygotic pairs. Tests of striatal 18-F-Dopa uptake in clinically unaffected mono- and dizygotic co-twins did not alter the ratio between the concordance rates. Though not excluded by the twin studies, multifactorial (or polygenic) inheritance as well as mitochondrial inheritance are at present less likely to cover most of the inheritance pattern in familial LB parkinsonism. Instead, autosomal dominant inheritance with reduced penetrance is the most probable inheritance pattern for most of the reported pedigrees. Molecular genetic investigations have to consider the biochemical basis of the age- and region-specific pathology of PD. The first analyses of linkage and allelic associations gave inconclusive results in sporadic and familial PD. The hunt for metabolic factors that link geno- and phenotype expression in PD will continue.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"8 1-2","pages":"1-37"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02250916","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18891796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 42
A new metabolic pathway of L-threo-3,4-dihydroxyphenylserine, a precursor amino acid of norepinephrine, in the brain. Studies by in vivo microdialysis. 去甲肾上腺素前体氨基酸l -三邻3,4-二羟基苯基丝氨酸在大脑中的新代谢途径。体内微透析研究。
W Maruyama, D Nakahara, M Naoi

The metabolism and the effects of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) were studied in the rat brain striatum by in vivo microdialysis. In the brain L-threo-DOPS was metabolized by 3 different enzymes; aromatic L-amino acid decarboxylase, catechol-O-methyltransferase, and DOPS-aldolase. DOPS-aldolase was the main enzyme which metabolizes L-threo-DOPS. The amounts of the metabolites by L-amino acid decarboxylase (norepinephrine and its metabolites) were 0.4% of the total amounts of metabolites detected in the dialysate, while those by catechol-O-methyltransferase, 2.1%, and by DOPS-aldolase, 97.5%, after 100 min perfusion of L-threo-DOPS. L-threo-DOPS was found to increase extracellular levels of dopamine and serotonin, and to inhibit monoamine catabolism in the brain. Inhibition of DOPS-aldolase should improve its effectiveness as the supplement therapy of norepinephrine.

采用体内微透析的方法,研究了l -苏-3,4-二羟基苯基丝氨酸(l -苏- dops)在大鼠脑纹状体中的代谢及其作用。脑内L-threo-DOPS被3种不同的酶代谢;芳香l-氨基酸脱羧酶,儿茶酚- o -甲基转移酶和dops醛缩酶。dops醛缩酶是l -三o- dops代谢的主要酶。l- 3 - dops灌注100 min后,l-氨基酸脱羧酶(去甲肾上腺素及其代谢物)代谢物含量占透析液代谢物总量的0.4%,儿茶酚- o-甲基转移酶代谢物含量为2.1%,dops -醛缩酶代谢物含量为97.5%。L-threo-DOPS被发现可以增加细胞外多巴胺和血清素的水平,并抑制大脑中单胺的分解代谢。抑制dops醛缩酶可提高其作为去甲肾上腺素补充治疗的有效性。
{"title":"A new metabolic pathway of L-threo-3,4-dihydroxyphenylserine, a precursor amino acid of norepinephrine, in the brain. Studies by in vivo microdialysis.","authors":"W Maruyama,&nbsp;D Nakahara,&nbsp;M Naoi","doi":"10.1007/BF02252660","DOIUrl":"https://doi.org/10.1007/BF02252660","url":null,"abstract":"<p><p>The metabolism and the effects of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) were studied in the rat brain striatum by in vivo microdialysis. In the brain L-threo-DOPS was metabolized by 3 different enzymes; aromatic L-amino acid decarboxylase, catechol-O-methyltransferase, and DOPS-aldolase. DOPS-aldolase was the main enzyme which metabolizes L-threo-DOPS. The amounts of the metabolites by L-amino acid decarboxylase (norepinephrine and its metabolites) were 0.4% of the total amounts of metabolites detected in the dialysate, while those by catechol-O-methyltransferase, 2.1%, and by DOPS-aldolase, 97.5%, after 100 min perfusion of L-threo-DOPS. L-threo-DOPS was found to increase extracellular levels of dopamine and serotonin, and to inhibit monoamine catabolism in the brain. Inhibition of DOPS-aldolase should improve its effectiveness as the supplement therapy of norepinephrine.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"7 1","pages":"21-33"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02252660","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19560260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
A comparison of multiplex and simplex families with Alzheimer's disease/senile dementia of Alzheimer type within a well defined population. 在一个明确界定的人群中患有阿尔茨海默病/阿尔茨海默型老年痴呆的多重和单一家族的比较
I Alafuzoff, E Almqvist, R Adolfsson, S Lake, W Wallace, D A Greenberg, B Winblad

A study was made on 150 clinically demented patients presenting at autopsy at Umeå University Hospital in Sweden. In 90 of the cases dementia was considered to be primary in nature and of these forty six per cent (41 cases), fulfilled both the clinical and histopathological criteria for the diagnosis of Alzheimer's disease/Senile dementia of Alzheimer type (AD/SDAT). The families of these 41 AD/SDAT cases were then studied, and a family history obtained through interviews with multiple family informants and from civil and medical records. Additional diseased family members suffering from progressive dementia (multiplex families) were observed in 12 probands out of 41 (29%). Multiplex families exhibited similar clinical and histopathological characteristics as simplex families containing a single affected individual. The secondary cases in the multiplex families exhibited similar demographic and clinical characteristics as the probands. 39% of the multiplex and 14% of the simplex cases had an early age of onset of the disease, that was under 65 years. The overall prevalence of progressive dementia disorders in the 41 families was 5.9%. The prevalence of a progressive dementia disorder was 11% in the multiplex families (14% for the early onset cases) and 3.5% in the simplex families (2% for the early onset cases). The prevalence of progressive dementia disorder for family members who had passed the mean age of the onset of the disease for their family, was 45% for multiplex and 18% for simplex families. Furthermore the incidence rate for dementia was significantly higher (p < 0.005) in multiplex families (5.5 per 1,000 person years) when compared to simplex families (2.5 per 1,000 person years). No differences could be seen in parental age at birth of the diseased when comparing the two sets of families. However in multiplex families the duration of the disease was significantly (p < 0.025) shorter, in subjects with parental age at birth over 35 years compared to those with a parental age under 35 years. The multiplex families contained significantly (p < 0.025) larger sibships; and showed a significantly lower age of onset for the disease (p < 0.001), and a significantly longer duration of disease (p < 0.05) compared to the simplex families. A significant intra familial correlation of age at disease onset was observed in both sets of the families.

对150名在瑞典尤梅夫大学医院进行尸检的临床痴呆患者进行了研究。在90例病例中,痴呆症被认为是原发性的,其中46%(41例)符合阿尔茨海默病/阿尔茨海默型老年痴呆症(AD/SDAT)的临床和组织病理学诊断标准。然后对这41例AD/SDAT病例的家庭进行研究,并通过与多名家庭举报人的访谈以及从民事和医疗记录中获得家族史。41个先证者中有12个(29%)观察到患有进行性痴呆的患病家庭成员(多重家族)。多重家族表现出与单纯性家族相似的临床和组织病理学特征。多重家族继发病例表现出与先证者相似的人口学和临床特征。39%的多发性病例和14%的单纯性病例发病年龄较早,即65岁以下。41个家庭中进行性痴呆的总体患病率为5.9%。进行性痴呆的患病率在多重家族中为11%(早发病例为14%),在单纯性家族中为3.5%(早发病例为2%)。在超过发病平均年龄的家庭成员中,进行性痴呆的患病率在多重家庭中为45%,在单纯性家庭中为18%。此外,与单一家庭(每1000人年2.5人)相比,多重家庭(每1000人年5.5人)的痴呆发病率显著更高(p < 0.005)。当比较两组家庭时,在患病患者出生时的父母年龄没有差异。然而,在多重家庭中,父母出生时年龄大于35岁的受试者的疾病持续时间明显短于父母出生时年龄小于35岁的受试者(p < 0.025)。多元家庭的兄弟姐妹数显著大于(p < 0.025);与单纯性家庭相比,单纯性家庭的发病年龄明显降低(p < 0.001),病程明显延长(p < 0.05)。在两组家庭中均观察到发病年龄的显著家族内相关性。
{"title":"A comparison of multiplex and simplex families with Alzheimer's disease/senile dementia of Alzheimer type within a well defined population.","authors":"I Alafuzoff,&nbsp;E Almqvist,&nbsp;R Adolfsson,&nbsp;S Lake,&nbsp;W Wallace,&nbsp;D A Greenberg,&nbsp;B Winblad","doi":"10.1007/BF02252663","DOIUrl":"https://doi.org/10.1007/BF02252663","url":null,"abstract":"<p><p>A study was made on 150 clinically demented patients presenting at autopsy at Umeå University Hospital in Sweden. In 90 of the cases dementia was considered to be primary in nature and of these forty six per cent (41 cases), fulfilled both the clinical and histopathological criteria for the diagnosis of Alzheimer's disease/Senile dementia of Alzheimer type (AD/SDAT). The families of these 41 AD/SDAT cases were then studied, and a family history obtained through interviews with multiple family informants and from civil and medical records. Additional diseased family members suffering from progressive dementia (multiplex families) were observed in 12 probands out of 41 (29%). Multiplex families exhibited similar clinical and histopathological characteristics as simplex families containing a single affected individual. The secondary cases in the multiplex families exhibited similar demographic and clinical characteristics as the probands. 39% of the multiplex and 14% of the simplex cases had an early age of onset of the disease, that was under 65 years. The overall prevalence of progressive dementia disorders in the 41 families was 5.9%. The prevalence of a progressive dementia disorder was 11% in the multiplex families (14% for the early onset cases) and 3.5% in the simplex families (2% for the early onset cases). The prevalence of progressive dementia disorder for family members who had passed the mean age of the onset of the disease for their family, was 45% for multiplex and 18% for simplex families. Furthermore the incidence rate for dementia was significantly higher (p < 0.005) in multiplex families (5.5 per 1,000 person years) when compared to simplex families (2.5 per 1,000 person years). No differences could be seen in parental age at birth of the diseased when comparing the two sets of families. However in multiplex families the duration of the disease was significantly (p < 0.025) shorter, in subjects with parental age at birth over 35 years compared to those with a parental age under 35 years. The multiplex families contained significantly (p < 0.025) larger sibships; and showed a significantly lower age of onset for the disease (p < 0.001), and a significantly longer duration of disease (p < 0.05) compared to the simplex families. A significant intra familial correlation of age at disease onset was observed in both sets of the families.</p>","PeriodicalId":16466,"journal":{"name":"Journal of Neural Transmission - Parkinson's Disease and Dementia Section","volume":"7 1","pages":"61-72"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02252663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19560263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
期刊
Journal of Neural Transmission - Parkinson's Disease and Dementia Section
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1