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Effective disinfecting of negative pressure pipelines of DCUs reduces the risk of cross infection in dental care. 对 DCU 负压管道进行有效消毒可降低牙科护理中交叉感染的风险。
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2024-01-07 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2023.2299538
Xuerong Lv, Ke Yan, Xiang Han, Feiyang Wang, Yuzhuo Ma, Shougang Zhang, Xiaoqian Wang

Objectives: Microbial contamination of various accessory parts of the dental chair units (DCUs) is an essential source of cross infection, while the accessories of the crucial suction function are usually overlooked. In this study, we aim to find an effective disinfectant and a cost-effective method to remove bacterioplankton and bacterial biofilm deposited in the negative pressure suction pipelines to control cross infection during dental treatment.

Methods: Double-chain quaternary ammonium salt disinfectant (Orotol Plus®), 3% hydrogen peroxide solution plus multi-enzyme cleaning agent and chlorine disinfectant are used to clean and disinfect the negative pressure pipelines of DCUs. Microbiological examinations, air condition detection, corrosion tests and gene sequencing are performed.

Results: Little bacteria grow in the pipelines disinfected with double-chain quaternary ammonium salt disinfectants, destruction of biofilms in these pipelines appears, and multi-resistant bacteria cannot be detected. Minimal damage to metal sheets and fittings is caused by double-chain quaternary ammonium salt disinfectants.

Conclusion: Double-chain quaternary ammonium salt disinfectant has excellent bactericidal ability and anti-biofilm effect, and it is less corrosive to the fittings of the pipelines. Thus, the double-chain quaternary ammonium salt disinfectant is a potential novel disinfectant for negative pressure suction pipelines of DCUs to control cross infection during dental treatment.

Clinical significance: It is essential to add all these data to our dental practice to control cross infection with a broader landscape.

目的:牙科治疗椅(DCU)各种附件的微生物污染是交叉感染的一个重要来源,而关键的抽吸功能附件通常被忽视。在这项研究中,我们旨在寻找一种有效的消毒剂和经济有效的方法,以去除沉积在负压吸引管道中的浮游细菌和细菌生物膜,从而控制牙科治疗过程中的交叉感染:方法:使用双链季铵盐消毒剂(Orotol Plus®)、3%过氧化氢溶液加多酶清洁剂和氯消毒剂对 DCU 的负压管道进行清洁和消毒。进行了微生物检查、空气状况检测、腐蚀测试和基因测序:结果:在使用双链季铵盐消毒剂消毒的管道中几乎没有细菌生长,管道中的生物膜被破坏,无法检测到多重抗性细菌。双链季铵盐消毒剂对金属板和配件的损害极小:结论:双链季铵盐消毒剂具有良好的杀菌能力和抗生物膜效果,对管道配件的腐蚀性较小。因此,双链季铵盐消毒剂是一种潜在的新型消毒剂,可用于 DCU 的负压吸引管道,以控制牙科治疗过程中的交叉感染:临床意义:将所有这些数据添加到我们的牙科实践中,以更广阔的视野控制交叉感染是非常必要的。
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引用次数: 0
N-acyl homoserine lactones lactonase est816 suppresses biofilm formation and periodontitis in rats mediated by Aggregatibacter actinomycetemcomitans. N-acyl homoserine lactones lactonase est816 可抑制放线杆菌介导的大鼠生物膜形成和牙周炎。
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2024-01-07 eCollection Date: 2024-01-01 DOI: 10.1080/20002297.2023.2301200
Zelda Ziyi Zhao, Junmin Wang, Xinpai Liu, Zezhi Wang, Xianyu Zheng, Wuli Li, Tianfan Cheng, Jing Zhang

Aims: The current study aimed to explore the adjuvant therapeutic effect of N-acyl homoserine lactones (AHLs)-lactonase est816 on Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) biological behaviors and periodontitis progression.

Methods: The inhibitory properties of est816 were detected by live/dead bacterial staining, scanning electron microscope (SEM), crystal-violet staining and reverse-transcription quantitative PCR (RT-qPCR). Biocompatibility of est816 on human gingival fibroblasts (HGFs) and human gingival epithelial cells (HGEs) was evaluated by CCK8 and ELISA. The ligature-induced periodontitis model was established in rats. Micro computed tomography and immunohistochemical and histological staining served to evaluate the effect of est816 on the prevention of periodontitis in vivo.

Results: est816 significantly attenuated biofilm formation, reduced the mRNA expression of cytolethal distending toxin, leukotoxin and poly-N-acetyl glucosamine (PNAG) and downregulated expressions of interleukin-6 and tumor necrosis factor-α with low cell toxicity. In vivo investigations revealed est816 decreased alveolar bone resorption, suppressed matrix metalloproteinase-9 expression and increased osteoprotegerin expression.

Conclusion: est816 inhibited A. actinomycetemcomitans biofilm formation and virulence release, resulting in anti-inflammation and soothing of periodontitis in rats, indicating that est816 could be investigated in further research on periodontal diseases.

目的:本研究旨在探讨N-酰基均丝氨酸内酯(AHLs)-内酯酶est816对放线菌属(A. actinomycetemcomitans)生物行为和牙周炎进展的辅助治疗作用:方法:通过活/死细菌染色、扫描电子显微镜(SEM)、水晶紫染色和反转录定量 PCR(RT-qPCR)检测 est816 的抑制特性。通过 CCK8 和 ELISA 评估了 est816 对人牙龈成纤维细胞(HGFs)和人牙龈上皮细胞(HGEs)的生物相容性。在大鼠身上建立了结扎诱导的牙周炎模型。结果表明:est816能显著减少生物膜的形成,降低细胞致死性膨胀毒素、白细胞毒素和聚-N-乙酰葡糖胺(PNAG)的mRNA表达,下调白细胞介素-6和肿瘤坏死因子-α的表达,且细胞毒性较低。结论:est816能抑制放线菌生物膜的形成和毒力释放,从而抗炎和舒缓大鼠牙周炎,表明est816可用于牙周疾病的进一步研究。
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引用次数: 0
Detection of Epstein-Barr virus infection in primary junctional epithelial cell cultures 在原代交界上皮细胞培养物中检测 Epstein-Barr 病毒感染
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2024-01-03 DOI: 10.1080/20002297.2023.2301199
Lilit Tonoyan, Charles V Olivieri, Marlène Chevalier, Robert Marsault, Alain Doglio
Junctional epithelium (JE) provides the front-line defense against pathogens invading periodontium. The breakdown of the JE barrier is the hallmark of periodontitis. Recent studies have implicated ...
交界上皮(JE)是抵御病原体入侵牙周的第一道防线。JE 屏障的破坏是牙周炎的标志。最近的研究表明...
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引用次数: 0
The importance of biofilm contamination control for dental unit waterlines: a multicenter assessment of the microbiota diversity of biofilm in dental unit waterlines 牙科单位水管生物膜污染控制的重要性:牙科单位水管生物膜微生物群多样性的多中心评估
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-12-30 DOI: 10.1080/20002297.2023.2299496
Piaopiao Chen, Jiang Zeng, Feiruo Hong, Cong Li, Huimin Wang, Xuefen Yu
The biofilm formation in Dental Unit Waterlines (DUWLs) could become an important cause of infection during dental care, which could put immunocompromised individuals at risk of cross-infection. Th...
牙科设备水管(DUWL)中形成的生物膜可能成为牙科护理过程中感染的重要原因,从而使免疫力低下的人面临交叉感染的风险。这...
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引用次数: 0
MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis-infected human gingival fibroblasts 抑制 MAT2A 可抑制受牙龈卟啉单胞菌感染的人牙龈成纤维细胞的炎症反应
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-12-15 DOI: 10.1080/20002297.2023.2292375
Lishan Jiang, Jingwen Li, Kun Ji, Lang Lei, Houxuan Li
Methionine adenosyl transferase II alpha (MAT2A) is the key enzyme to transform methionine into S-adenosylmethionine (SAM), the main methylgroup donor involved in the methylation. The purpose of ou...
蛋氨酸腺苷转移酶 II alpha(MAT2A)是将蛋氨酸转化为 S-腺苷蛋氨酸(SAM)的关键酶,SAM 是参与甲基化的主要甲基基团供体。MAT2A的作用是...
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引用次数: 0
Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis 研究牙周炎细菌和病毒病原体的牙龈组织模型
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-12-12 DOI: 10.1080/20002297.2023.2292382
Anna Golda, Anna Gasiorek, Ewelina Dobosz, Zuzanna Oruba, Richard J. Lamont, Jan Potempa, Joanna Koziel
Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model...
三维(3D)组织模型弥合了传统二维细胞培养和动物模型之间的差距。本研究的目的是建立一个器官型三维牙龈(OTG)模型。
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引用次数: 0
Oxidative stress response: a critical factor affecting the ecological competitiveness of Streptococcus mutans 氧化应激反应:影响变异链球菌生态竞争力的关键因素
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-12-12 DOI: 10.1080/20002297.2023.2292539
Xingqun Cheng, Xin Xu, Xuedong Zhou, Jia Ning
Oral microecological balance is closely associated with the development of dental caries. Oxidative stress is one of the important factors regulating the composition and structure of the oral micro...
口腔微生态平衡与龋病的发生密切相关。氧化应激是调节口腔微细胞组成和结构的重要因素之一。
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引用次数: 0
Deficiency of dltD contributes to increased autolysis in Streptococcus mutans 缺乏 dltD 会导致变异链球菌自溶增加
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-12-10 DOI: 10.1080/20002297.2023.2290221
Jingyun Du, Shan Huang, Yijun Li, Minjing Wu, Shuai Chen, Ling Zhan, Xiaojing Huang
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引用次数: 0
Ultrasmall epibiont Nanosynbacter lyticus strain TM7x and host bacteria transcriptional activity after initial host parasitism 超小附生纳米溶菌TM7x与寄主初始寄生后寄主细菌转录活性的关系
IF 4.5 2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-11-30 DOI: 10.1080/20002297.2023.2287349
Erik L Hendrickson, Batbileg Bor, Kristopher A. Kerns, Lujia Cen, Wenyuan Shi, Xuesong He, Jeffrey S McLean
Oral Saccharibacteria Nanosynbacter lyticus strain TM7× lives as an ultrasmall epibiont on the surface of its host, Schaalia odontolytica strain XH001. Establishing this interaction is a poorly und...
口腔溶牙纳米合杆菌tm7x以超小型表观生物的形式存在于宿主溶牙沙利亚菌XH001的表面。建立这种互动是一个很糟糕的……
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引用次数: 0
Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis 亚致死浓度的氯己定通过破坏活性氧和金属离子稳态来抑制白色念珠菌的生长
2区 医学 Q2 MICROBIOLOGY Pub Date : 2023-11-09 DOI: 10.1080/20002297.2023.2278937
Qian Jiang, Yuchen Deng, Shuaihu Li, Deqin Yang, Li Tao
Candida albicans is a normal resident of the human oral cavity. It is also the most common fungal pathogen, causing various oral diseases, particularly in immunocompromised individuals. Chlorhexidine digluconate (CHG) is a broad-spectrum antimicrobial agent widely used in dental practice and has been recommended to treat oral candidiasis. However, its action mechanism against the fungal pathogen C. albicans remains poorly understood. The aim of the present study was to investigate the effect of CHG at sub-lethal concentrations against C. albicans. CHG inhibited the growth of C. albicans in a dose- and time-dependent manner. Cells treated with CHG exhibited altered membrane permeability, reduced metabolic activity, and enhanced metal ion and reactive oxygen species (ROS) accumulation. Copper-sensing transcription factor Mac1, iron-sensing transcription factors Sfu1 and Sef2, and copper transporter Ctr1 regulated intracellular metal ion and ROS homeostasis in response to CHG. Deletion of MAC1, SFU1, or SEF2 increased intracellular ROS production and cell susceptibility to CHG. This study revealed a novel mechanism by which CHG induced apoptosis of C. albicans cells through the disruption of metal ion and ROS homeostasis, which may help to identify new targets for fungal infections.
白色念珠菌是人类口腔的正常居民。它也是最常见的真菌病原体,引起各种口腔疾病,特别是在免疫功能低下的个体中。二光酸氯己定(CHG)是一种广泛应用于牙科实践的广谱抗菌药物,已被推荐用于治疗口腔念珠菌病。然而,其对真菌病原体白色念珠菌的作用机制尚不清楚。本研究的目的是探讨亚致死浓度的CHG对白色念珠菌的作用。CHG抑制白色念珠菌生长呈剂量和时间依赖性。经CHG处理的细胞表现出膜通透性改变,代谢活性降低,金属离子和活性氧(ROS)积累增强。铜敏感转录因子Mac1、铁敏感转录因子Sfu1和Sef2以及铜转运体Ctr1调节细胞内金属离子和ROS稳态响应CHG。MAC1、SFU1或SEF2的缺失增加了细胞内ROS的产生和细胞对CHG的易感性。本研究揭示了CHG通过破坏金属离子和ROS稳态诱导白色念珠菌细胞凋亡的新机制,这可能有助于发现真菌感染的新靶点。
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Journal of Oral Microbiology
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