Journal of Ovarian Research最新文献

{"title":"Clinical outcomes of natural cycle IVF/M with the different sizes of dominant follicle at the time of hCG triggering.","authors":"Jianhua Li, Jing Chen, Tingting Jiao, Qingchen Cui, Jianye Wang, Yanbin Cheng, Ye Wang, Ye Xu, Ri-Cheng Chian, Youzhu Li, Shuang Tian","doi":"10.1186/s13048-025-01914-w","DOIUrl":"10.1186/s13048-025-01914-w","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"38"},"PeriodicalIF":4.2,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12860154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145863325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erzhi Tiangui formula increases the number of high-quality blastocysts in patients with advanced age by regulating the Nrf2/HO-1 signaling pathway: a randomized controlled study.","authors":"Li Xiufang, Wang Zhongqing, Wang Huidan, Zheng Ye, Han Xu, Chen Wei, Wu Haicui, Lian Fang","doi":"10.1186/s13048-025-01940-8","DOIUrl":"10.1186/s13048-025-01940-8","url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress (OS) is a significant challenge in the in vitro embryo culture of patients of advanced age. The Erzhi Tiangui (EZTG) formula has been shown to alleviate OS-induced apoptosis in granulosa cells (GCs) of patients undergoing in vitro fertilization (IVF) cycles. This study aimed to investigate the protective effects and underlying mechanisms of the EZTG formula on OS and apoptosis in GCs of patients with advanced age.</p><p><strong>Methods: </strong>A total of 240 patients aged 35 to 42 years were randomly assigned to either the experimental group (EZTG formula) or the control group (placebo). The experimental group received 3 g of EZTG formula three times daily, starting from the menstrual period preceding the IVF cycle for approximately 45 days, while the control group received a placebo. The primary outcome measure was the number of high-quality embryos, while secondary outcomes included additional indicators related to oocytes, embryos, and pregnancy outcomes. GCs were collected from advanced-age patients during oocyte retrieval. Nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) are key signaling molecules associated with OS prevention. These molecules potentially link the EZTG formula, OS mitigation, and embryo quality through Nrf2 activation. We examined the protein and mRNA levels of Nrf2/HO-1 and the activities of downstream antioxidant enzymes in GCs to elucidate the underlying mechanisms. Apoptosis-related protein and gene expression involved in the Nrf2/HO-1 signaling pathway were analyzed using Western blotting and quantitative reverse-transcription polymerase chain reaction (qPCR). Additionally, the active glutathione (GSH) levels in GCs were determined using enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>The number of high-quality embryos and fertilization rates were significantly increased in the EZTG group compared to the control group (both P < 0.05). GCs from the EZTG group showed significant upregulation of GSH and adenosine triphosphate (ATP) levels (both P < 0.05). Western blot and qPCR analyses indicated that the EZTG formula alleviated OS by activating the Nrf2/HO-1 signaling pathway. Specifically, the protein levels of Keap1, Bax, and Caspase-3 were decreased, while those of Nrf2, HO-1, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and Bcl-2 were increased in the EZTG group compared to the control group (all P < 0.05). Similarly, the mRNA levels of Keap1, Bax, and Caspase-3 were significantly decreased, while Nrf2, HO-1, and Bcl-2 were significantly increased in the EZTG group (all P < 0.05). These findings suggest that the EZTG formula mitigates OS through the Nrf2/HO-1 signaling pathway, ultimately improving embryo quality in IVF-derived embryos.</p><p><strong>Conclusion: </strong>The EZTG formula can improve embryo quality in patients of advanced age by activating the Nrf2/HO-1 signaling path","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"37"},"PeriodicalIF":4.2,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12860082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145863358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atractylenolide III mitigates polycystic ovary syndrome by activating FDX1-mediated proliferation of ovarian granulocyte cells via PI3K/AKT/mTOR.","authors":"Nan Zhou, Wei Tai, Wenqiang Lv, Qing He, Shiwen He, Kexin Chen, Qing Wang, Jingbo Zhang, Suyan Li, Yijuan Cao, Xueyan Zhou","doi":"10.1186/s13048-025-01941-7","DOIUrl":"10.1186/s13048-025-01941-7","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"44"},"PeriodicalIF":4.2,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12870249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145863322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNA circMYBL2 regulates the progression of ovarian cancer through miR-195-5P/BIRC5 axis.","authors":"Binxin Liu, Yadan Fan, Chunyan Lv, Gen Zeng, Miaomiao Cai, Yinglian Pan, Qingchun Deng","doi":"10.1186/s13048-025-01946-2","DOIUrl":"10.1186/s13048-025-01946-2","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"40"},"PeriodicalIF":4.2,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12866556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145856907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy of ionomycin - induced artificial oocyte activation in preventing fertilization failure in an ICSI cycle: an eight - year retrospective study.","authors":"Ming Li, Nan Zhang, Yuehao Sun, Jin Huang, Qin Li, Junsheng Li, Ping Liu, Rong Li","doi":"10.1186/s13048-025-01954-2","DOIUrl":"https://doi.org/10.1186/s13048-025-01954-2","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145856857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic modeling of endometriosis-associated ovarian cancer based on molecular signatures: a retrospective study.","authors":"Ming Wang, Jianqing Xu, Jianfen Cui, Shuiqing Xu, Yumei Wu","doi":"10.1186/s13048-025-01937-3","DOIUrl":"10.1186/s13048-025-01937-3","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"35"},"PeriodicalIF":4.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12859853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145856854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the metabolome of follicular fluid in GnRH agonist versus antagonist protocols during in vitro fertilization cycles.","authors":"Chengguang Sun, Shen Zhang, Xianglan Tang, Ting-Li Han, Fan He","doi":"10.1186/s13048-025-01933-7","DOIUrl":"10.1186/s13048-025-01933-7","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"36"},"PeriodicalIF":4.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12860088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145849974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of PLAU in granulosa cells disrupts steroid hormone synthesis and promotes apoptosis by activating NF-κB signaling pathway in PCOS.","authors":"Wenxiu Chen, Hanzhi Zhang, Mingxin Jiang, Xing Su, Jingfei Chen, Jianlin Chen","doi":"10.1186/s13048-025-01930-w","DOIUrl":"https://doi.org/10.1186/s13048-025-01930-w","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145856918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between serum antinuclear antibodies and premature ovarian insufficiency: a meta-analysis.","authors":"Tejunzhang Pi, Zuxian Hu, Lei Luo, Zhengqi Liu","doi":"10.1186/s13048-025-01945-3","DOIUrl":"10.1186/s13048-025-01945-3","url":null,"abstract":"<p><strong>Background: </strong>Autoimmune dysfunction is a recognized contributor to primary ovarian insufficiency (POI), and antinuclear antibodies (ANA) are widely used to assess autoimmune activity. Although many studies have examined the association between ANA and POI, their findings remain inconsistent. This meta-analysis aims to clarify the correlation between ANA positivity and POI and to evaluate the potential of ANA as a serological marker of autoimmune involvement in POI.</p><p><strong>Methods: </strong>We systematically searched PubMed, EMBASE, Cochrane Library, CNKI, VIP, and WanFang Data for case-control studies published up to August 2024. Manual reference screening was also performed. Studies comparing antinuclear antibody (ANA) positivity between women with primary ovarian insufficiency (POI) and controls were included. A random-effects model was used to calculate pooled odds ratios (ORs) with 95% confidence intervals (CIs). Heterogeneity and publication bias were assessed using the I² statistic and Begg's test.</p><p><strong>Results: </strong>According to the analysis of all 13 studies, ANA positivity was significantly associated with POI (OR 2.57, 95% CI: 1.62-4.08, Z = 4.001, P < 0.001). No significant heterogeneity was observed in the primary outcome (I² = 11.9%). Subgroup analyses revealed statistically significant associations in the following: (1) FSH > 40 U/L subgroup (OR = 2.74, 95% CI: 1.78-4.21, I² = 0%); (2) age < 35 subgroup (OR = 2.83, 95% CI: 1.73-4.63, I² = 3.8%); (3) indirect immunofluorescence (IIF) subgroup (OR = 3.22, 95% CI: 1.28-8.09, I² = 34.6%) and dot immunogold filtration assay (DIGFA) subgroup (OR = 3.84, 95% CI: 1.31-11.27, I² = 0%); (4) non-ovarian infertility controls (OR = 4.42, 95% CI: 1.25-15.60, I² = 0%), healthy controls (OR = 2.13, 95% CI:1.23-3.68, I² = 0%), and hypogonadotropic amenorrhea controls (OR = 6.62, 95% CI: 2.25-19.52, I² = 0%) groups.</p><p><strong>Conclusion: </strong>ANA positivity is significantly associated with an increased risk of POI, supporting its potential role as a serological marker for early screening. Routine ANA testing may be particularly valuable in women under 35 years of age or with FSH > 40 IU/L. Future large-scale studies should focus on optimizing ANA detection methods (e.g., enzyme-linked immunosorbent assay [ELISA] vs. IIF), quantifying titers, and evaluating nuclear patterns to improve diagnostic accuracy and clinical applicability.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"34"},"PeriodicalIF":4.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12859911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated plasma glucose links intrafollicular bile acids to altered embryological outcomes in IVF patients.","authors":"Natascha Berger, Giovanny Rodriguez-Blanco, Katharina Brugger, Bettina Amtmann, Neli Hofer, Martina Kollmann, Irmgard Oreskovic, Katharina Eberhard, Samuele Suraci, Slave Trajanoski, Markus Herrmann, Ursula Hiden, Herbert Fluhr","doi":"10.1186/s13048-025-01939-1","DOIUrl":"10.1186/s13048-025-01939-1","url":null,"abstract":"","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":" ","pages":"39"},"PeriodicalIF":4.2,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12866166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145846748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}