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Prospective study on biochemical markers causing recurrent miscarriages in Iraqi women of reproductive age 伊拉克育龄妇女引起反复流产的生化标志物的前瞻性研究
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-17
None Ruqaya Kahtan Abbas, None Amani Jasim, None Qater Al Nada Ali Kanaem AL Ibady
Objective: To compare the levels of elemental calcium, vitamin D3, malondialdehyde and super oxide dismutaseenzymes in serum of women having recurrent miscarriages with normal healthy pregnant women.Method: The prospective study was conducted at Al-Karkh Maternity Hospital, Baghdad, Iraq, and Al-Alawia MaternityHospital, Baghdad, Iraq, from February to August 2022, and comprised adult pregnant women having recurrentmiscarriages without being hypertensive or diabetic in group A and normal healthy pregnant women in control groupB. Blood samples were obtained from all the subjects. Besides, placental tissues were obtained from some membersfrom both the groups. Levels of elemental calcium, vitamin D3, malondialdehyde and superoxide dismutase enzymesin serum were evaluated and compared between the groups. Malondialdehyde and super oxide dismutase enzymesin the placental tissues were also compared. Data was analysed using SPSS 20.Results: Of the 140 women, 70(50%) were in each of the 2 groups. The overall age range of the sample was 18-43years. Placental tissues were obtained from 40(57.14%) in group A and 30(42.85%) in group B. In group A, miscarriagewas reported in the first trimester in 45(62.3%) cases, 21(30%) in the second trimester and 4(5.7%) in the third trimester.Mean level of serum elemental calcium, vitamin D3, malondialdehyde and super oxide dismutase were significantlydifferent between the groups (p<0.05), and the same was the case with the placental tissues (p<0.05).Conclusion: The causes of recurrent miscarriage in women could be attributed to changes in the levels of elementalcalcium, vitamin D3, super oxide dismutase and malondialdehyde in serum and placental tissues.Keywords: Pregnancy, Abortion, Spontaneous, Malondialdehyde, Cholecalciferol, Placenta, Vitamins, Oxides.
目的:比较反复流产妇女与正常健康孕妇血清中钙、维生素D3、丙二醛和超氧化物歧化酶的水平。方法:前瞻性研究于2022年2 - 8月在伊拉克巴格达Al-Karkh妇产医院和伊拉克巴格达Al-Alawia妇产医院进行,A组为无高血压或糖尿病的复发性流产成年孕妇,b组为正常健康孕妇。采集了所有受试者的血液样本。此外,还从两组的一些成员身上获得了胎盘组织。测定各组血清钙、维生素D3、丙二醛和超氧化物歧化酶水平并进行比较。并比较了胎盘组织中丙二醛和超氧化物歧化酶的含量。数据采用SPSS 20进行分析。结果:140例患者中,两组各70例(50%)。样本的总体年龄范围为18-43岁。A组胎盘组织40例(57.14%),b组30例(42.85%)。A组妊娠早期流产45例(62.3%),妊娠中期流产21例(30%),妊娠晚期流产4例(5.7%)。各组间血清元素钙、维生素D3、丙二醛、超氧化物歧化酶平均水平差异有统计学意义(p < 0.05),胎盘组织平均水平差异有统计学意义(p < 0.05)。结论:妇女反复流产的原因可能与血清和胎盘组织中元素钙、维生素D3、超氧化物歧化酶和丙二醛水平的变化有关。关键词:妊娠,流产,自发,丙二醛,胆钙化醇,胎盘,维生素,氧化物。
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引用次数: 0
Mother’s knowledge and attitudes regarding poliomyelitis eradication among a sample in Bab Al Muadham health centre in Baghdad city, Iraq 在伊拉克巴格达的Bab Al Muadham卫生中心,母亲对根除小儿麻痹症的知识和态度
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-32
None Entsar Khaleel Sameen, None Shatha Mahmood Niazy, None Asmaa Ibadi Salman, None Sahar Al Kareem Alabbas Al Ghnaimawi
Objective: To determine knowledge and attitude and practice of mothers towards poliomyelitis vaccination.Method: The cross-sectional study was conducted from October 2019 to March 2020 at Bab Al-Moadham HealthCentre, Baghdad, Iraq, and comprised mothers regardless of age who were visiting the child care unit with at least 1child aged <5 years. Data was collected through a questionnaire. Data was analysed using SPSS 10.Results: There were 150 mothers with a mean age of 31.08±9.31 years. The largest age group was 20-29 years 90(60%)(p<0.05). There were 100(66.6%) subjects from urban areas, and 67(44.6%) had at least a college degree (p<0.05). Themost common source of information was television 110(73.3%), and 135(90%) subjects agreed that polio vaccine forthe child was a necessity. Overall assessment of the sample was good, with a percentile global means of the scorevalue of 50.30±9.81.Conclusion: Urban, educated and working mothers aged 20-29 years were more knowledgeable about polio than the rest.Keywords: Health, Knowledge, Attitudes, Poliomyelitis, Vaccination, Eradication.
目的:了解母亲对脊髓灰质炎疫苗接种的知识、态度和做法。方法:横断面研究于2019年10月至2020年3月在伊拉克巴格达的Bab Al-Moadham健康中心进行,研究对象包括至少有1名5岁儿童的母亲,无论年龄大小。通过问卷调查收集数据。数据采用SPSS 10进行分析。结果:150例产妇,平均年龄31.08±9.31岁。20 ~ 29岁为最大年龄组,占60% (p < 0.05)。100名受试者(66.6%)来自城市地区,67名受试者(44.6%)具有大学以上学历(p < 0.05)。最常见的信息来源是电视110(73.3%),135(90%)受试者同意儿童接种脊髓灰质炎疫苗是必要的。样本的总体评价良好,评分值的百分位数全球平均值为50.30±9.81。结论:20-29岁的城市、受过教育和有工作的母亲对脊髓灰质炎的了解程度高于其他母亲。关键词:健康,知识,态度,脊髓灰质炎,疫苗接种,根除
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引用次数: 0
Evaluation of cytotoxicity and Apoptotic effects of Simarouba glauca on the Prostate Cancer Cell Lines PC3 青光眼对前列腺癌细胞PC3的细胞毒性及凋亡作用的研究
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-24
None Omar Sadik Shalal, None Sajjad Mohsin Irayyif
Objective: To investigate the anti-proliferative and pro-apoptotic activities of simarouba glauca on prostate cancercell lines PC-3.Method: This cross-sectional study was conducted at Iraqi centre for Genetic and Cancer Research from 15th Januaryto 20th June 2021. The evaluation was designed through apoptotic and membrane depolarisation assays. Methanolextract at 0.29ug/ml, 29ug/ml and 290ug/ml doses were used, while rifampicin acted as the positive control anddimethyl sulfoxide acted as the negative control. Real time gene expression was also done to evaluate the levels ofmessenger ribonucleic acid expression. Data was analysed using SPSS 16.Results: The methanol extract inhibited 50% of the PC-3 cells at a mean concentration of 35.24±0.23ug/mL comparedto 15.31±1.83ug/mL for positive control. The extracts pushed PC3 cells significantly into early apoptosis comparedto the positive control (p<0.05). The extracts greatly boosted the percentage of mitochondrial membrane potentialdepolarisation. Methanol extract was found to under-express both vascular endothelial growth factor A by 5-fold andarachidonate 5-lipoxygenase by 4-fold compared to positive control (p<0.05).Conclusion: Simarouba glauca methanol extracts were found to differentially control proliferation and apoptosis incancer cell lines PC-3.Keywords: Methanol, Plant extracts, Gene expression, Prostatic neoplasms, Phytochemicals, Drug design.
目的:探讨青光珠对前列腺癌细胞PC-3的抑制增殖和促凋亡作用。方法:本横断面研究于2021年1月15日至6月20日在伊拉克遗传和癌症研究中心进行。通过凋亡和膜去极化实验设计评价。采用0.29ug/ml、29ug/ml和290ug/ml剂量的甲醇提取物,利福平为阳性对照,二甲亚砜为阴性对照。实时基因表达以评估信使核糖核酸的表达水平。数据采用SPSS 16进行分析。结果:甲醇提取物对PC-3细胞的抑制率为50%,平均浓度为35.24±0.23ug/mL,阳性对照为15.31±1.83ug/mL。与阳性对照相比,提取物显著促进PC3细胞早期凋亡(p<0.05)。提取物可显著提高线粒体膜电位去极化率。与阳性对照相比,甲醇提取物的血管内皮生长因子A和花生四烯酸5-脂氧合酶的表达量分别减少了5倍和4倍(p < 0.05)。结论:青花香茅甲醇提取物对癌细胞PC-3的增殖和凋亡具有不同程度的控制作用。关键词:甲醇,植物提取物,基因表达,前列腺肿瘤,植物化学物质,药物设计
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引用次数: 0
Investigation of anthraquinone-producing Trichoderma reesei by highperformance liquid chromatography (HPLC) 高效液相色谱法研究产蒽醌木霉
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-25
None Zeyad Khalaf Hussein, None Ghada Basil Alomashi, None Khaled Yassin Zakair
Objective: To investigate the isolates of Trichoderma that produce anthraquinones by High-performance LiquidChromatography (HPLC).Methods: Trichoderma specimen were collected from Wassit Soil and identified dependent on morphological featuresand tested for production of anthraquinones by HPLC, Trichoderma isolate which produced a high concentration ofanthraquinones diagnosed for the species using PCR-ITS region.Results: Ten isolates were identified as Trichoderma according to morphological and microscopic features. Resultsthree Trichoderma isolates show differences between the concentrations of anthraquinones among the ten isolates.The total concentration of this compound in the extracts of specimens 1, 2 and 3 were (7.765ug/ml), (2.308ug/ml),and (4.977ug/ml) respectively. At the final concentration of Trichoderma isolates, genomic DNA have been extracted(400 to 600 ug) / (2 to 3g) fresh mycelium, and with a concentration of (1.6 to1.8), and the results of amplifyingTrichoderma DNA samples by using ITS-1 and ITS-4 showed a single unique band consistent with T. reesei F48-03, Whichwith other isolates of the Trichoderma were missing, were identified successfully.Conclusion: For identification and phylogenetic classification of Trichoderma, DNA-based methods that provide usefulclassification information are presently used. For several years, most T. spp. is regarded as a single species due to theirmorphological similarity. This research used ITS markers to distinguish genotypes within T. spp. because of amplifyinga distinct, naturally determined locus with a couple of T.reesei -specific oligonucleotide primers. This research wascarried out to provide supporting evidence for the long-standing antimicrobial use of anthraquinone.Keywords: DNA, Primers, Trichoderma, Phylogeny, Soil, Chromatography, Liquid, Polymerase, Reaction,Anthraquinones, Mycelium, Plant, Genomics.
目的:采用高效液相色谱法对产蒽醌的木霉进行分离。方法:从瓦西特土壤中采集木霉标本,根据形态特征对其进行鉴定,并通过高效液相色谱法检测其产蒽醌类物质的含量。结果:10株分离株经形态学和显微特征鉴定为木霉。结果3株木霉在10株菌株中蒽醌类化合物含量存在差异。该化合物在标本1、2、3提取物中的总浓度分别为(7.765ug/ml)、(2.308ug/ml)、(4.977ug/ml)。在最终浓度为(1.6 ~ 1.8)的条件下,提取(400 ~ 600 ug) / (2 ~ 3g)新鲜菌丝体的基因组DNA,利用ITS-1和ITS-4扩增木霉DNA样品,结果显示出一条与T. reesei F48-03一致的独特条带,与其他木霉分离菌株缺失的条带一致。结论:对于木霉的鉴定和系统发育分类,目前采用基于dna的方法提供了有用的分类信息。多年来,由于它们在形态上的相似性,大多数弓形虫被认为是一个单一的物种。本研究使用ITS标记来区分T. spp的基因型,因为它扩增了一个独特的,自然确定的位点与一对T.reesei特异性寡核苷酸引物。本研究旨在为蒽醌的长期抗菌应用提供支持证据。关键词:DNA,引物,木霉,系统发育,土壤,色谱,液体,聚合酶,反应,蒽醌类,菌丝体,植物,基因组学
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引用次数: 0
Premature infant parents’ Need at Neonatal Intensive Care Unit in Erbil City, Iraq: A descriptive cross-sectional Study 伊拉克埃尔比勒市新生儿重症监护病房早产儿父母的需求:一项描述性横断面研究
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-12
None Kafia Hashim Karim, None Norhan Zeki Shakir
Objectives: To assess the perceived needs of parents who had premature infants in a neonatal intensive care setting.Method: The descriptive cross-sectional study was conducted at the Raparin Teaching Hospital for Children, Erbil,Iraq, from April to June 2021, and comprised parents with premature infants who were enrolled during their infants’stay at the neonatal intensive care unit. Data was collected using a two-part questionnaire. The first part entailed aninterview exploring socio-demographic data, while the second part was a self-report questionnaire adapted from theCritical Care Family Needs Inventory, and had five domains; support, information, proximity, assurance, and comfort.Data was analysed using SPSS 25.Results: There were 57 sets of parents and premature neonates. The mean gestational age of the neonates was33.37±3.29 weeks, mean age of fathers was 31.44±7.24 years, and mean age of mothers was 27.51±6.19 years. Amongthe neonates, 31(54.4%) were boys, and 32(56.1%) had been delivered through caesarean section. Of the total 114mother and father, 106(93%) required assurance, 98(86%) needed comfort, and 96(84.2%) confirmed proximity as themost essential need. Besides, 84(73.7%) required information and 79(69.3%) required support.Conclusion: Parents of premature infants hospitalised in neonatal intensive care unit needed assurance, comfort,proximity, information and support in order of priority.Key Words: Premature, Infant, Parents, Erbil.
目的:评估早产儿父母在新生儿重症监护环境中的感知需求。方法:描述性横断面研究于2021年4月至6月在伊拉克埃尔比勒的Raparin儿童教学医院进行,研究对象是在新生儿重症监护病房住院期间登记的早产儿父母。数据收集采用两部分的问卷调查。第一部分包括对社会人口统计数据的访谈,而第二部分是根据重症监护家庭需求清单改编的自我报告问卷,共有五个领域;支持、信息、亲近、保证和舒适。数据采用SPSS 25进行分析。结果:共57对父母及早产儿。新生儿平均胎龄为33.37±3.29周,父亲平均年龄为31.44±7.24岁,母亲平均年龄为27.51±6.19岁。男婴31例(54.4%),剖宫产32例(56.1%)。在114位父母中,106位(93%)需要保证,98位(86%)需要安慰,96位(84.2%)确认亲近是最基本的需求。其中84个(73.7%)需要信息,79个(69.3%)需要支持。结论:新生儿重症监护病房早产儿家长对保障、舒适、亲近、信息和支持的需求依次为优先级。关键词:早产儿,婴儿,父母,埃尔比勒
{"title":"Premature infant parents’ Need at Neonatal Intensive Care Unit in Erbil City, Iraq: A descriptive cross-sectional Study","authors":"None Kafia Hashim Karim, None Norhan Zeki Shakir","doi":"10.47391/jpma.iq-12","DOIUrl":"https://doi.org/10.47391/jpma.iq-12","url":null,"abstract":"Objectives: To assess the perceived needs of parents who had premature infants in a neonatal intensive care setting.Method: The descriptive cross-sectional study was conducted at the Raparin Teaching Hospital for Children, Erbil,Iraq, from April to June 2021, and comprised parents with premature infants who were enrolled during their infants’stay at the neonatal intensive care unit. Data was collected using a two-part questionnaire. The first part entailed aninterview exploring socio-demographic data, while the second part was a self-report questionnaire adapted from theCritical Care Family Needs Inventory, and had five domains; support, information, proximity, assurance, and comfort.Data was analysed using SPSS 25.Results: There were 57 sets of parents and premature neonates. The mean gestational age of the neonates was33.37±3.29 weeks, mean age of fathers was 31.44±7.24 years, and mean age of mothers was 27.51±6.19 years. Amongthe neonates, 31(54.4%) were boys, and 32(56.1%) had been delivered through caesarean section. Of the total 114mother and father, 106(93%) required assurance, 98(86%) needed comfort, and 96(84.2%) confirmed proximity as themost essential need. Besides, 84(73.7%) required information and 79(69.3%) required support.Conclusion: Parents of premature infants hospitalised in neonatal intensive care unit needed assurance, comfort,proximity, information and support in order of priority.Key Words: Premature, Infant, Parents, Erbil.","PeriodicalId":16673,"journal":{"name":"Journal of Pakistan Medical Association","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134944099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of COVID-19 pandemic on immunisation services performance in Sharqat, Saladin Governorate, Iraq 2019冠状病毒病大流行对伊拉克萨拉丁省Sharqat免疫服务绩效的影响
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-03
None Muna Abdul Kadhum Zeidan, None Suhair Mohammed Hassoon
Objective: To determine the frequency of vaccination delays and the causes of such delays during the coronavirusdisease-2019 pandemic.Method: The cross-sectional study was conducted from December 15, 2020, to July 20, 2021, at health centres in theSharqat area of Saladin Governorate in Iraq, and comprised parents who brought their child for immunisation duringcoronavirus disease-2019 pandemic. Data was gathered via direct interviews using a pre-designed questionnaire.Data was analysed using SPSS 19.Results: Of the 150 respondents, 84(56%) were from urban areas, and 65(42%) had higher education levels. The associationwas significant with level of education, socio-economic status of the family and availability of transportation (p<0.05).Conclusion: The vaccination process got disrupted during the coronavirus disease-2019 pandemic in Iraq.Keywords: COVID-19, Pandemic, Immunisation, Performance.
目的:了解2019冠状病毒病大流行期间疫苗接种延误的频率及其原因。方法:横断面研究于2020年12月15日至2021年7月20日在伊拉克萨拉丁省sharqat地区的卫生中心进行,研究对象包括在2019冠状病毒病大流行期间带孩子接种疫苗的父母。使用预先设计的问卷通过直接访谈收集数据。数据采用SPSS 19进行分析。结果:在150名受访者中,84人(56%)来自城市地区,65人(42%)受过高等教育。与受教育程度、家庭社会经济地位和交通便利程度的相关性显著(p < 0.05)。结论:伊拉克2019冠状病毒病大流行期间疫苗接种过程中断。关键词:COVID-19,大流行,免疫,绩效
{"title":"Impact of COVID-19 pandemic on immunisation services performance in Sharqat, Saladin Governorate, Iraq","authors":"None Muna Abdul Kadhum Zeidan, None Suhair Mohammed Hassoon","doi":"10.47391/jpma.iq-03","DOIUrl":"https://doi.org/10.47391/jpma.iq-03","url":null,"abstract":"Objective: To determine the frequency of vaccination delays and the causes of such delays during the coronavirusdisease-2019 pandemic.Method: The cross-sectional study was conducted from December 15, 2020, to July 20, 2021, at health centres in theSharqat area of Saladin Governorate in Iraq, and comprised parents who brought their child for immunisation duringcoronavirus disease-2019 pandemic. Data was gathered via direct interviews using a pre-designed questionnaire.Data was analysed using SPSS 19.Results: Of the 150 respondents, 84(56%) were from urban areas, and 65(42%) had higher education levels. The associationwas significant with level of education, socio-economic status of the family and availability of transportation (p<0.05).Conclusion: The vaccination process got disrupted during the coronavirus disease-2019 pandemic in Iraq.Keywords: COVID-19, Pandemic, Immunisation, Performance.","PeriodicalId":16673,"journal":{"name":"Journal of Pakistan Medical Association","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134944104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence of Overweight and Obesity among Pre-adolescence school children at Al- Nasiriya city, Iraq 伊拉克Al- Nasiriya市青春期前学龄儿童超重和肥胖患病率
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-15
None Rawaa Kamel Abd, None Sameeha Naser Abd, None Makki Hasan Kadhim, None Sana Rouis, None Mahdi Boutrif
Objective: To examine the sociodemographic factors and determine the prevalence of overweight and obesity amongstudents in their early adolescent years.Method: The cross-sectional study was conducted at four governmental intermediate schools located in Al-Nasiriyacity of Thi-Qar Governorate in Iraq from January 2 to February 15, 2021, and comprised students of either gender aged12-14 years. Data was gathered using a self-designed questionnaire. Body mass index was calculated after measuringthe subjects’ weight in kilograms on a properly calibrated digital scale and their height in centimetres on an appropriatetape meter scale. Data was analysed using SPSS 23.Results: Of the 218 subjects, 124(56.88%) were girls and 94(43.12%) were boys. Overall, 100(45.87%) subjects wereaged 13 years. There were 71(32.56%) overweight and 30(13.76%) obese subjects. Age and gender were significantlyassociated with body mass index (p<0.05).Conclusion: Students in their early adolescent years were relatively more likely to be overweight and obese. Age andgender had a correlation with body mass index.Keywords: Overweight, Body mass, Paediatric, Obesity, Demography.
目的:探讨青少年早期超重和肥胖的社会人口学因素,确定青少年早期超重和肥胖的患病率。方法:横断面研究于2021年1月2日至2月15日在伊拉克Thi-Qar省al - nasiriycity的四所政府中级学校进行,包括12-14岁的男女学生。使用自行设计的问卷收集数据。身体质量指数的计算是在经过适当校准的数字秤上测量受试者的体重(公斤)和身高(厘米)后进行的。数据采用SPSS 23进行分析。结果:218名被试中,女生124名(56.88%),男生94名(43.12%)。总体而言,100名(45.87%)受试者年龄为13岁。超重71例(32.56%),肥胖30例(13.76%)。年龄、性别与体重指数有显著相关性(p < 0.05)。结论:青少年早期的学生相对来说更容易超重和肥胖。年龄和性别与体重指数有相关性。关键词:超重,体重,儿科,肥胖,人口学。
{"title":"Prevalence of Overweight and Obesity among Pre-adolescence school children at Al- Nasiriya city, Iraq","authors":"None Rawaa Kamel Abd, None Sameeha Naser Abd, None Makki Hasan Kadhim, None Sana Rouis, None Mahdi Boutrif","doi":"10.47391/jpma.iq-15","DOIUrl":"https://doi.org/10.47391/jpma.iq-15","url":null,"abstract":"Objective: To examine the sociodemographic factors and determine the prevalence of overweight and obesity amongstudents in their early adolescent years.Method: The cross-sectional study was conducted at four governmental intermediate schools located in Al-Nasiriyacity of Thi-Qar Governorate in Iraq from January 2 to February 15, 2021, and comprised students of either gender aged12-14 years. Data was gathered using a self-designed questionnaire. Body mass index was calculated after measuringthe subjects’ weight in kilograms on a properly calibrated digital scale and their height in centimetres on an appropriatetape meter scale. Data was analysed using SPSS 23.Results: Of the 218 subjects, 124(56.88%) were girls and 94(43.12%) were boys. Overall, 100(45.87%) subjects wereaged 13 years. There were 71(32.56%) overweight and 30(13.76%) obese subjects. Age and gender were significantlyassociated with body mass index (p<0.05).Conclusion: Students in their early adolescent years were relatively more likely to be overweight and obese. Age andgender had a correlation with body mass index.Keywords: Overweight, Body mass, Paediatric, Obesity, Demography.","PeriodicalId":16673,"journal":{"name":"Journal of Pakistan Medical Association","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134944198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on the physiochemical and microbial content of drinking water in and around Al-Nasiriyah province 纳西里耶省及其周边地区饮用水理化及微生物含量研究
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-06
None Safa Fadhil, None Amani Jasim, None Amal Kamel
Objective: To identify microbial contents of water by physical and chemical analysesin different regions in a Middle East country.Methods: The prospective, cross-sectional study was conducted in and around Al-Nassirya province ofIraq from March to July 2021, and comprised drinking water samples taken from water stations in citycentre as well as Al-Dawaya, Al-Shatrah and Al-Nasr districts in the outskirts. Analysis included estimationof turbidity, temperatures, potential of hydrogen, total hardness, and estimation of chlorine, calcium andsodium measured in The-Qar university laboratories, Data was analysed using SPSS version 17. T-test wasused to compare the mean values. A p-value of Less than or Equal to 0.05 was considered significant.Results: Of the 10 water samples 1(10%) was collected from the city centre, 4(40%) from Al-Dawaya district,2(20%) from Shatra district and 3(30%) from Al-Nasr district. Significant differences were found in thesamples collected from city centre compared to those from the countryside (p<0.29). Turbidity was less(1.7) in samples from the city centre compared to the three countryside water stations. High levels ofchlorine (26), concentration of calcium (199) and sodium (479) were moderately high in samples fromAl-Nasiriya city. Microbial growth was detected in samples from Al-Nasr and Al-Dawaya districts whereEscherichia coli, staphylococcus aureus and cryptosporidiumparrum were detected in some water stations.Conclusion: Countryside water stations showed changes in standard physiochemical normal values andcontamination of drinking water with microorganisms.Keywords: Chlorine, Cryptosporidium parvum, Staphylococcus aureus, Cryptosporidiosis,Cryptosporidium, Escherichia coli, Sodium.
目的:对中东某国不同地区水中微生物含量进行理化分析。方法:前瞻性横断面研究于2021年3月至7月在伊拉克Al-Nassirya省及其周边地区进行,包括从市中心以及郊区Al-Dawaya、Al-Shatrah和Al-Nasr地区的供水站采集的饮用水样本。分析包括估计浊度,温度,氢电位,总硬度,以及估计氯,钙和钠在喀尔大学实验室测量,数据分析使用SPSS版本17。采用t检验比较平均值。小于或等于0.05被认为是显著的的p值。结果:10个水样中1个(10%)来自市中心,4个(40%)来自Al-Dawaya区,2个(20%)来自Shatra区,3个(30%)来自Al-Nasr区。与从农村收集的样本相比,从市中心收集的样本存在显著差异(p<0.29)。与三个农村水站相比,来自市中心的样本浊度较低(1.7)。在al - nasiriya市的样品中,氯(26)、钙(199)和钠(479)浓度偏高。在Al-Nasr和Al-Dawaya地区的样品中检测到微生物生长,在一些水站检测到大肠杆菌、金黄色葡萄球菌和隐孢子虫。结论:农村供水站的标准理化正常值和饮用水微生物污染情况均有变化。关键词:氯,细小隐孢子虫,金黄色葡萄球菌,隐孢子虫病,隐孢子虫,大肠杆菌,钠
{"title":"Study on the physiochemical and microbial content of drinking water in and around Al-Nasiriyah province","authors":"None Safa Fadhil, None Amani Jasim, None Amal Kamel","doi":"10.47391/jpma.iq-06","DOIUrl":"https://doi.org/10.47391/jpma.iq-06","url":null,"abstract":"Objective: To identify microbial contents of water by physical and chemical analysesin different regions in a Middle East country.Methods: The prospective, cross-sectional study was conducted in and around Al-Nassirya province ofIraq from March to July 2021, and comprised drinking water samples taken from water stations in citycentre as well as Al-Dawaya, Al-Shatrah and Al-Nasr districts in the outskirts. Analysis included estimationof turbidity, temperatures, potential of hydrogen, total hardness, and estimation of chlorine, calcium andsodium measured in The-Qar university laboratories, Data was analysed using SPSS version 17. T-test wasused to compare the mean values. A p-value of Less than or Equal to 0.05 was considered significant.Results: Of the 10 water samples 1(10%) was collected from the city centre, 4(40%) from Al-Dawaya district,2(20%) from Shatra district and 3(30%) from Al-Nasr district. Significant differences were found in thesamples collected from city centre compared to those from the countryside (p<0.29). Turbidity was less(1.7) in samples from the city centre compared to the three countryside water stations. High levels ofchlorine (26), concentration of calcium (199) and sodium (479) were moderately high in samples fromAl-Nasiriya city. Microbial growth was detected in samples from Al-Nasr and Al-Dawaya districts whereEscherichia coli, staphylococcus aureus and cryptosporidiumparrum were detected in some water stations.Conclusion: Countryside water stations showed changes in standard physiochemical normal values andcontamination of drinking water with microorganisms.Keywords: Chlorine, Cryptosporidium parvum, Staphylococcus aureus, Cryptosporidiosis,Cryptosporidium, Escherichia coli, Sodium.","PeriodicalId":16673,"journal":{"name":"Journal of Pakistan Medical Association","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134944362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pregnant women’s knowledge toward the effects of drugs on mother health’s and foetus during pregnancy in Fatima Al Zahra hospital, Baghdad, Iraq 在伊拉克巴格达的Fatima Al Zahra医院,孕妇对怀孕期间药物对母亲健康和胎儿影响的了解
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-22
None Shatha Mahmood Niazi, None Abeer Abed Gatea
Objective: To assess pregnant women’s awareness on medication use and its effects on their health and the healthof their unborn child.Methods: A descriptive cross-sectional study on pregnant women who frequently visited the outpatient clinic at theFatima Al-Zahra Hospital was conducted using a non-probability sampling technique. A two-part questionnaire, thefirst of which inquired about socio-demographic information and the second inquired about mothers’ awareness ofthe effects of medications on their health and the health of the foetus while they were pregnant, was used to collectdata through direct interviews. The content validity of the instrument was established with the assistance of 20specialists. Cronbach’s alpha Coefficient was used to assess the instrument scales’ reliability. A Likert item was used toassess respondents’ understanding of a certain topic or statement. Using multivariate logistic regression analysis, thestrength of the independent correlations between different components and the desired results was evaluated.Results: The study’s findings show that participants’ awareness of how medications affect a pregnant woman’s healthand the foetus had a bad overall mean score. On average, they were 27.4 8.34 years old. A 75 (75%) of the participantsin the study were over the age of 21, had a secondary education, and were working in some capacity. A 50 (50%) ofwomen had had three to five pregnancies, and 48 (48%) were in the third trimester. 11 (11%) of them utilized themedication illicitly.Conclusion: Maternal awareness of medication use is statistically strongly associated with a number of factorsinfluencing outcomes. In fact, pregnant adults over the age of 21 had little knowledge about it, little education, andused the drug without a prescription. The results of the study highlight the need for increased communication aboutmedication use with expectant mothers in order to enhance their understanding and reduce self-medication.Keywords: Knowledge, Pregnancy, Mothers, Fetus, Medication DOI: https://doi.org/10.47391/JPMA.IQ-22
目的:了解孕妇用药意识及其对孕妇和胎儿健康的影响。方法:采用非概率抽样技术,对经常到法蒂玛扎赫拉医院门诊就诊的孕妇进行描述性横断面研究。问卷分为两部分,第一部分询问社会人口统计信息,第二部分询问母亲在怀孕期间对药物对其健康和胎儿健康的影响的认识,通过直接访谈收集数据。在20名专家的协助下,建立了该仪器的内容效度。采用Cronbach’s alpha系数评价量表的信度。李克特项目被用来评估受访者对某个主题或陈述的理解。使用多元逻辑回归分析,评估不同成分与期望结果之间的独立相关强度。结果:研究结果表明,参与者对药物如何影响孕妇健康和胎儿的认识总体平均得分较低。他们的平均年龄为27.4 - 8.34岁。研究中75%的参与者年龄在21岁以上,受过中等教育,并且有一定的工作能力。50名(50%)女性有过三到五次怀孕,48名(48%)处于妊娠晚期。其中11人(11%)非法使用药物。结论:孕产妇用药意识与影响结局的多种因素有统计学上的密切关系。事实上,21岁以上的孕妇对它知之甚少,教育程度也很低,而且没有处方就服用了这种药物。研究结果强调了与准妈妈加强药物使用沟通的必要性,以提高她们的理解,减少自我用药。关键词:知识,妊娠,母亲,胎儿,药物DOI: https://doi.org/10.47391/JPMA.IQ-22
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引用次数: 0
Identification of Leishmania species isolated from cutaneous leishmaniasis using molecular techniques in some provinces of Iraq 利用分子技术鉴定伊拉克部分省份皮肤利什曼病分离种
Pub Date : 2023-10-06 DOI: 10.47391/jpma.iq-14
None Manar Kareem, None Sundus Nsaif Al Huchaimi, None Rasha Fadhl Obaid, None Huda A. Saleh
Objectives: To detect Leishmania species in selected regions in Iraq.Method: The cross-sectional study was conducted from December 2021 to March 2022 at Al-Hashimyah GeneralHospital in Babylon province, and Al Diwaniya Education Hospital in AL-Qadisiah province of Iraq. The samplecomprised patients with lesions suggestive of cutaneous leishmaniasis, as assessed by dermatologists. Direct smearwas used for parasitological examination by lesion aspiration. Molecular technique was used for the characterisationof parasite genus and species. Positive direct smear specimens were analysed by nested-polymerase chain reactionusing internal transcribed spacer-1 ribosomal ribonucleic acid gene.Results: Of the 120 specimens tested, 100(83.33%) tested positive; 59(59%) males and 41(41%) females. Most of theinfections were leishmania major 92(92%), followed by leishmania tropica 8(8%). There were 80(80%) dry lesions and20(20%) wet.Conclusions: Cutaneous leishmaniasis were mostly of tropica and major varieties in the studied areas.Keywords: Parasites, Dermatologists, Genes, rRNA, Cutaneous, Leishmania, Polymerase DNA.
目的:对伊拉克选定地区的利什曼原虫进行检测。方法:横断面研究于2021年12月至2022年3月在伊拉克巴比伦省Al- hashimyah综合医院和Al- qadisiah省Al- Diwaniya教育医院进行。经皮肤科医生评估,样本包括有提示皮肤利什曼病病变的患者。采用直接涂片法,病变抽吸进行寄生虫学检查。采用分子技术对寄生虫属和种进行了鉴定。直接涂片阳性标本采用内转录间隔-1核糖体核糖核酸基因巢式聚合酶链反应进行分析。结果:120例标本中阳性100例(83.33%);男性59人(59%),女性41人(41%)。感染最多的是大利什曼原虫92例(92%),其次是热带利什曼原虫8例(8%)。干性病变80例(80%),湿性病变20例(20%)。结论:研究地区皮肤利什曼病以热带地区为主,品种较多。关键词:寄生虫,皮肤科医生,基因,rRNA,皮肤,利什曼原虫,聚合酶DNA。
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Journal of Pakistan Medical Association
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