{"title":"Cardiac Arrest as the Initial Presentation of Leigh Syndrome Associated with Novel NDUFS1 Mutation","authors":"Na Hyun Lee, Do-Hoon Kim, J. Byun","doi":"10.46308/kmj.2023.00017","DOIUrl":"https://doi.org/10.46308/kmj.2023.00017","url":null,"abstract":"","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"148 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124718782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Retinal and Choroidal Vasculature Before and After Patch Occlusion Treatment Using Optical Coherence Tomography Angiography in Patients with Amblyopia","authors":"Jae-Gon Kim, Se Youp Lee, Dong Cheol Lee","doi":"10.46308/kmj.2022.00241","DOIUrl":"https://doi.org/10.46308/kmj.2022.00241","url":null,"abstract":"","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"32 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131452456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Knowledge and Attitude about Alternative Treatment in Cancer Patient","authors":"Jee Weon Lee, Deog-Young Lee, DaeHyun Kim","doi":"10.46308/kmj.2022.00248","DOIUrl":"https://doi.org/10.46308/kmj.2022.00248","url":null,"abstract":"","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122153719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
People who recover from the novel coronavirus diseases 2019 (COVID-19) complain of psychological symptoms such as stress, anxiety, and depression by psycho-stress due to social stigma in the early stage of the epidemic. To assess the psychological stigma of early and late epidemic periods, stigma scale was compared between the first epidemic period (February-May 2020, n = 20) and the fifth epidemic period (February-May 2022, n = 21) post-recovery COVID-19 patients in outpatient clinic. The average total stigma score was significantly higher (79.6 ± 18.16) in post-recovery COVID-19 patients of the early (first) epidemic period compared to late (5th) epidemic period patients (37.5 ±10.99). Four subscales were significantly higher in early epidemic period compared to late epidemic period (enacted stigma 27.5 ± 7.25 vs. 13.0±4.18, disclosure concerns 19.5 ± 9.38 vs. 9.4 ± 2.99, negative self-image 16.2 ± 8.10 vs. 8.1±2.47, concern with public attitudes 16.6 ± 6.95 vs. 7.0 ± 2.01). In those who recovered from the early COVID-19 epidemic period, patients experienced significantly higher social stigma stress compared to the late epidemic period. In the early period of novel virus epidemics, the risk of personal stigmatization and stress should be prevented in public policy. Socio-psychological assessment and treatment should be considered for the patient in addition to physical symptoms.
新型冠状病毒感染症(COVID-19)的恢复期患者，在疫情初期因社会污名化而产生的心理压力中，出现了紧张、焦虑、抑郁等心理症状。为评估疫情前期和后期的心理耻辱感，比较第一次疫情期(2020年2月- 5月，n = 20)和第五次疫情期(2022年2月- 5月，n = 21)门诊康复后COVID-19患者的耻辱感量表。初(1)期康复后患者的平均总污名分(79.6±18.16)明显高于末(5)期患者(37.5±10.99)分。4项量表(制定污名27.5±7.25 vs. 13.0±4.18，披露关切19.5±9.38 vs. 9.4±2.99，消极自我形象16.2±8.10 vs. 8.1±2.47，公众态度关切16.6±6.95 vs. 7.0±2.01)均显著高于流行后期。在COVID-19流行早期康复的患者中，与流行后期相比，患者的社会耻辱压力显着增加。在新型病毒流行的早期，应在公共政策中预防个人污名化和压力的风险。除了身体症状外，还应考虑对患者进行社会心理评估和治疗。
{"title":"Post-recovery Stigma in Early and Late COVID-19 Epidemic","authors":"Daehyun Kim","doi":"10.46308/kmj.2022.00171","DOIUrl":"https://doi.org/10.46308/kmj.2022.00171","url":null,"abstract":"People who recover from the novel coronavirus diseases 2019 (COVID-19) complain of psychological symptoms such as stress, anxiety, and depression by psycho-stress due to social stigma in the early stage of the epidemic. To assess the psychological stigma of early and late epidemic periods, stigma scale was compared between the first epidemic period (February-May 2020, n = 20) and the fifth epidemic period (February-May 2022, n = 21) post-recovery COVID-19 patients in outpatient clinic. The average total stigma score was significantly higher (79.6 ± 18.16) in post-recovery COVID-19 patients of the early (first) epidemic period compared to late (5th) epidemic period patients (37.5 ±10.99). Four subscales were significantly higher in early epidemic period compared to late epidemic period (enacted stigma 27.5 ± 7.25 vs. 13.0±4.18, disclosure concerns 19.5 ± 9.38 vs. 9.4 ± 2.99, negative self-image 16.2 ± 8.10 vs. 8.1±2.47, concern with public attitudes 16.6 ± 6.95 vs. 7.0 ± 2.01). In those who recovered from the early COVID-19 epidemic period, patients experienced significantly higher social stigma stress compared to the late epidemic period. In the early period of novel virus epidemics, the risk of personal stigmatization and stress should be prevented in public policy. Socio-psychological assessment and treatment should be considered for the patient in addition to physical symptoms.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115627366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salvianolic acid B (Sal B) is one of the most active hydrophilic compounds extracted from Salvia miltiorrhiza root. Previous in vitro and in vivo studies demonstrate the ability of Sal B to modulate adipocyte differentiation. However, the lipid-modulating effect and mechanism of Sal B in adipocytes remain controversial. Here we investigated the regulatory effect and mode of action of Sal B on lipid accumulation in 3T3-L1 preadipocyte differentiation. Lipid droplet (LD) accumulation and triglyceride (TG) content during 3T3-L1 preadipocyte differentiation were measured by Oil Red O staining and AdipoRed assay. The growth inhibition during 3T3-L1 preadipocyte differentiation was measured by cell count analysis. Western blotting and real-time qPCR analysis were utilized to determine the protein and mRNA expression in the preadipocyte differentiation. Notably, in 3T3-L1 preadipocyte differentiation, treatment with Sal B at 100 M led to a marked decrease in LD accumulation and TG content without influencing cell growth. Sal B treatment (100 M) further reduced the expression and phosphorylation levels of adipogenic transcription factors, including CCAAT/enhancer-binding protein- (C/EBP-), peroxisome proliferator-activated receptor-gamma (PPAR)-, and signal transducer and activator of transcription (STAT)-3/5. Treatment with Sal B (100 M) also reduced the expression and phosphorylation levels of fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), two lipogenic enzymes and perilipin A, an LD-binding and stabilizing protein. These results collectively demonstrate that Sal B at 100 M strongly inhibits lipid accumulation in 3T3-L1 preadipocyte differentiation, mediated through regulation of the expression and phosphorylation levels of C/EBP-, PPAR-, STAT-3/5, FAS, ACC, and perilipin.
丹参酚酸B (Salvianolic acid B, Sal B)是从丹参根中提取的最具活性的亲水化合物之一。先前的体外和体内研究证明了Sal B调节脂肪细胞分化的能力。然而，salb在脂肪细胞中的脂质调节作用及其机制仍存在争议。本研究探讨了salb对3T3-L1前脂肪细胞分化过程中脂质积累的调控作用及其作用方式。采用Oil Red O染色和AdipoRed法测定3T3-L1前脂肪细胞分化过程中脂滴(LD)积累和甘油三酯(TG)含量。通过细胞计数分析检测3T3-L1前脂肪细胞分化过程中的生长抑制作用。Western blotting和real-time qPCR检测前脂肪细胞分化过程中蛋白和mRNA的表达。值得注意的是，在3T3-L1前脂肪细胞分化中，100M的Sal B处理导致LD积累和TG含量显著降低，但不影响细胞生长。Sal B处理(100M)进一步降低了脂肪生成转录因子的表达和磷酸化水平，包括CCAAT/增强子结合蛋白-(C/EBP-)、过氧化物酶体增殖物激活受体- γ (PPAR)-和转录信号转换器和激活因子(STAT)-3/5。用Sal B(100M)处理也降低了脂肪酸合成酶(FAS)和乙酰辅酶A羧化酶(ACC)这两种脂肪生成酶和perilipin A(一种ld结合和稳定蛋白)的表达和磷酸化水平。
{"title":"Anti-adipogenic Effect and Mechanism in 3T3-L1 Preadipocyte Differentiation by Salvianolic Acid B","authors":"Hyo-Shin Kwon, B. Jang","doi":"10.46308/kmj.2022.00213","DOIUrl":"https://doi.org/10.46308/kmj.2022.00213","url":null,"abstract":"Salvianolic acid B (Sal B) is one of the most active hydrophilic compounds extracted from Salvia miltiorrhiza root. Previous in vitro and in vivo studies demonstrate the ability of Sal B to modulate adipocyte differentiation. However, the lipid-modulating effect and mechanism of Sal B in adipocytes remain controversial. Here we investigated the regulatory effect and mode of action of Sal B on lipid accumulation in 3T3-L1 preadipocyte differentiation. Lipid droplet (LD) accumulation and triglyceride (TG) content during 3T3-L1 preadipocyte differentiation were measured by Oil Red O staining and AdipoRed assay. The growth inhibition during 3T3-L1 preadipocyte differentiation was measured by cell count analysis. Western blotting and real-time qPCR analysis were utilized to determine the protein and mRNA expression in the preadipocyte differentiation. Notably, in 3T3-L1 preadipocyte differentiation, treatment with Sal B at 100 M led to a marked decrease in LD accumulation and TG content without influencing cell growth. Sal B treatment (100 M) further reduced the expression and phosphorylation levels of adipogenic transcription factors, including CCAAT/enhancer-binding protein- (C/EBP-), peroxisome proliferator-activated receptor-gamma (PPAR)-, and signal transducer and activator of transcription (STAT)-3/5. Treatment with Sal B (100 M) also reduced the expression and phosphorylation levels of fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), two lipogenic enzymes and perilipin A, an LD-binding and stabilizing protein. These results collectively demonstrate that Sal B at 100 M strongly inhibits lipid accumulation in 3T3-L1 preadipocyte differentiation, mediated through regulation of the expression and phosphorylation levels of C/EBP-, PPAR-, STAT-3/5, FAS, ACC, and perilipin.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126111348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Smoking can be changed by health behavior education on the fact that unhealthy behaviors can cause chronic diseases and cancer and that it is important to identify health behaviors in smoking. We try to compare the health behaviors of combustible cigarette (CC), heated tobacco (HT) users, and quitters. Smoking behaviors were divided into three groups (CC, HT users, and quitters). The HT user group (n = 100) was selected among those who underwent a health examination in 2021-2022. CC smokers cohort group (n = 100) and quitters cohort (n = 100) were randomly selected from the same groups (age ± 2) who underwent a health examination in the same period. Sleep-related problems (snoring and sleep apnea), alcohol consumption, and exercise were compared in the CC group, HT group, and quitters group, respectively. Snoring was more common in the quitters group (27%) than in the CC users group (19%) and HT users group (18%). It can be related to weight gain during quitting tobacco use. Nondrinkers were more common in the CC users group (21%) than the HT users group (8%) and quitters group (10%). CC users seem to be more concerned about the health effects of drinking compared to HT users and quitters. Anaerobic exercise was different among groups, and aerobic exercise was not. HT users group did more aerobic exercise than CC users and quitters group. Differences in healthy behaviors among CC and HC users and quitters can be useful information for health education to smokers. Understanding smokers’ health behavior is important to smoking cessation counseling in clinical practice.
{"title":"Health Behaviors in Combustible Cigarette, Heated Tobacco Users and Quitters","authors":"B. Youn, S. Hong, Dae-Wook Kim","doi":"10.46308/kmj.2022.00157","DOIUrl":"https://doi.org/10.46308/kmj.2022.00157","url":null,"abstract":"Smoking can be changed by health behavior education on the fact that unhealthy behaviors can cause chronic diseases and cancer and that it is important to identify health behaviors in smoking. We try to compare the health behaviors of combustible cigarette (CC), heated tobacco (HT) users, and quitters. Smoking behaviors were divided into three groups (CC, HT users, and quitters). The HT user group (n = 100) was selected among those who underwent a health examination in 2021-2022. CC smokers cohort group (n = 100) and quitters cohort (n = 100) were randomly selected from the same groups (age ± 2) who underwent a health examination in the same period. Sleep-related problems (snoring and sleep apnea), alcohol consumption, and exercise were compared in the CC group, HT group, and quitters group, respectively. Snoring was more common in the quitters group (27%) than in the CC users group (19%) and HT users group (18%). It can be related to weight gain during quitting tobacco use. Nondrinkers were more common in the CC users group (21%) than the HT users group (8%) and quitters group (10%). CC users seem to be more concerned about the health effects of drinking compared to HT users and quitters. Anaerobic exercise was different among groups, and aerobic exercise was not. HT users group did more aerobic exercise than CC users and quitters group. Differences in healthy behaviors among CC and HC users and quitters can be useful information for health education to smokers. Understanding smokers’ health behavior is important to smoking cessation counseling in clinical practice.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"219 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132646832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ju, Shailashree Pachhapure, Amila Mufida, A-Reum Kim, D. Elmaleh, Sungwoong Choi, B. Jang
Inflammation is a common link in the pathophysiology of many neurological illnesses, including Alzheimer’s disease. Activated glial cells contribute to neuroinflammation by producing pro-inflammatory mediators. Naproxen and ibuprofen are nonsteroidal anti-inflammatory drugs with 2-aryl(s) propionic acid as a common pharmacophore. Here we designed a small series of naproxen and ibuprofen amide dimers and tested their effects on the expression of inducible nitric oxide synthase (iNOS), a neuroinflammatory enzyme in lipopolysaccharide (LPS)-stimulated BV2 mouse microglial cells. Of note, treatment with CNU 019, 020, 021, 023, 024, and 027 at 10 M markedly inhibited the LPS-induced iNOS expression in BV2 cells. CNU 024 was tested further at different concentrations to regulate the LPS-induced iNOS expression in BV2 cells. Treatment with CNU 024 at 5, 10, or 20 M dose-dependently suppressed the LPS-induced iNOS protein and mRNA expression levels in BV2 cells, in which maximal inhibition was seen at 20 M. CNU 024 treatment at doses tested further led to a concentration-dependent inhibition of the LPS-induced phosphorylation (activation) of p38 mitogen-activated protein kinase (MAPK) without influencing its total protein expression in BV2 cells, but it did not affect the LPS-induced activation of c-jun N-terminal kinase-1/2 and extracellular signal-regulated kinases-1/2 in these cells. In summary, our results demonstrate that CNU 024 inhibits the LPS-induced iNOS expression in BV2 cells, partly mediated by the inhibition of p38 MAPK. This work shows that CNU 024 could be a valuable ligand for further development as a potential drug candidate for treating neuroinflammatory pathologies.
{"title":"2-Aryl Propionic Acid Amide Modification of Naproxen and Ibuprofen Dimers for Anti-neuroinflammatory Activity in BV2 mouse Microglial Cells","authors":"H. Ju, Shailashree Pachhapure, Amila Mufida, A-Reum Kim, D. Elmaleh, Sungwoong Choi, B. Jang","doi":"10.46308/kmj.2022.00199","DOIUrl":"https://doi.org/10.46308/kmj.2022.00199","url":null,"abstract":"Inflammation is a common link in the pathophysiology of many neurological illnesses, including Alzheimer’s disease. Activated glial cells contribute to neuroinflammation by producing pro-inflammatory mediators. Naproxen and ibuprofen are nonsteroidal anti-inflammatory drugs with 2-aryl(s) propionic acid as a common pharmacophore. Here we designed a small series of naproxen and ibuprofen amide dimers and tested their effects on the expression of inducible nitric oxide synthase (iNOS), a neuroinflammatory enzyme in lipopolysaccharide (LPS)-stimulated BV2 mouse microglial cells. Of note, treatment with CNU 019, 020, 021, 023, 024, and 027 at 10 M markedly inhibited the LPS-induced iNOS expression in BV2 cells. CNU 024 was tested further at different concentrations to regulate the LPS-induced iNOS expression in BV2 cells. Treatment with CNU 024 at 5, 10, or 20 M dose-dependently suppressed the LPS-induced iNOS protein and mRNA expression levels in BV2 cells, in which maximal inhibition was seen at 20 M. CNU 024 treatment at doses tested further led to a concentration-dependent inhibition of the LPS-induced phosphorylation (activation) of p38 mitogen-activated protein kinase (MAPK) without influencing its total protein expression in BV2 cells, but it did not affect the LPS-induced activation of c-jun N-terminal kinase-1/2 and extracellular signal-regulated kinases-1/2 in these cells. In summary, our results demonstrate that CNU 024 inhibits the LPS-induced iNOS expression in BV2 cells, partly mediated by the inhibition of p38 MAPK. This work shows that CNU 024 could be a valuable ligand for further development as a potential drug candidate for treating neuroinflammatory pathologies.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117058600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jae-Hee Park, Kwangrak Park, Anna Bae, Han-Gyu Jeong, Jae-ho Lee
It is necessary to install ventilation facilities in the laboratory and to regularly monitor harmful gases including formalin for safe environment of the dissection laboratory. However, there are no indicators that can identify the current status of ventilation facilities, safety equipment, and protective equipment in the dissection laboratory. In this study, the status of safety management of anatomical lab at domestic medical, dental, and oriental medical universities are investigated through an online questionnaire. Of the total 32 universities, 7 universities (21.8%) regularly monitor harmful gases such as formalin in the dissection lab, 13 universities (40.6%) do it on an irregular basis, and 12 do not do it at all. Seven universities (21.8%) are using the exhaust-type dissection table, 24 universities (75%) are not using it. Regarding the need for standards for manpower and facilities in the management of the anatomy lab, 7 universities (21.8%) are mediocre, 21 universities are necessary (65.6%), and 4 universities (12.5%) are very necessary. The responsibility for anatomy lab is 27 universities (84.3%) of the schools that responded as head professors of the department of anatomy, 3 universities (9.3%) of technicians, and 2 universities (6.2%) of the dean of the medical school. Regarding the need for standards for the anatomical lab, 7 universities (21.8%) are very necessary, 21 universities (65.6%) are necessary, and 4 universities (12.5%) are mediocre. Based on this data, the standard for the quality improvement and safety of anatomical education should be prepared.
{"title":"Safety and Management Status of Anatomical Labs in Medical Schools","authors":"Jae-Hee Park, Kwangrak Park, Anna Bae, Han-Gyu Jeong, Jae-ho Lee","doi":"10.46308/kmj.2022.00150","DOIUrl":"https://doi.org/10.46308/kmj.2022.00150","url":null,"abstract":"It is necessary to install ventilation facilities in the laboratory and to regularly monitor harmful gases including formalin for safe environment of the dissection laboratory. However, there are no indicators that can identify the current status of ventilation facilities, safety equipment, and protective equipment in the dissection laboratory. In this study, the status of safety management of anatomical lab at domestic medical, dental, and oriental medical universities are investigated through an online questionnaire. Of the total 32 universities, 7 universities (21.8%) regularly monitor harmful gases such as formalin in the dissection lab, 13 universities (40.6%) do it on an irregular basis, and 12 do not do it at all. Seven universities (21.8%) are using the exhaust-type dissection table, 24 universities (75%) are not using it. Regarding the need for standards for manpower and facilities in the management of the anatomy lab, 7 universities (21.8%) are mediocre, 21 universities are necessary (65.6%), and 4 universities (12.5%) are very necessary. The responsibility for anatomy lab is 27 universities (84.3%) of the schools that responded as head professors of the department of anatomy, 3 universities (9.3%) of technicians, and 2 universities (6.2%) of the dean of the medical school. Regarding the need for standards for the anatomical lab, 7 universities (21.8%) are very necessary, 21 universities (65.6%) are necessary, and 4 universities (12.5%) are mediocre. Based on this data, the standard for the quality improvement and safety of anatomical education should be prepared.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117286250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cong Thuc Le, Giang Nguyen, H. Dong, So young Park, Y. Cho, Daehee Choi, Won Sun Park, Yoojin Lee, Ji-Young Lee, Eun-Hee Cho
Hepatic stellate cells (HSCs) play a key role in liver fibrosis. Succinate and succinate receptor (GPR91) signaling pathway are involved in the activation, proliferation, and migration of HSCs. We investigated whether succinate may induce hepatic fibrosis. The mice were randomly divided into 2 groups —the control group (chow diet-fed mice, n = 26) and sodium succinate group (2% sodium succinate + chow diet, n = 38). Each diet was provided for 16 weeks. Mice administered an oral diet of 2% sodium succinate for sixteen weeks lost body weight and had increased serum alanine transaminase and hepatic triglyceride contents compared to those in the control mice. Moreover, mice fed with sodium succinate showed increased expression of the alpha smooth muscle actin protein and gene in the liver at 8 weeks of feeding and increased fibrosis in their histology at 16 weeks of feeding. However, the expression of the GPR91 protein and mRNA increased at 4 weeks of feeding, but decreased at 8 and 16 weeks of feeding. These results suggest that an oral succinate diet could induce liver damage and liver fibrosis in mice and that GPR91 signaling might be an early marker or sensor of hepatic fibrosis development.
{"title":"Succinate Induces Liver Damage and Hepatic Fibrosis in a Mouse Model","authors":"Cong Thuc Le, Giang Nguyen, H. Dong, So young Park, Y. Cho, Daehee Choi, Won Sun Park, Yoojin Lee, Ji-Young Lee, Eun-Hee Cho","doi":"10.46308/kmj.2022.00143","DOIUrl":"https://doi.org/10.46308/kmj.2022.00143","url":null,"abstract":"Hepatic stellate cells (HSCs) play a key role in liver fibrosis. Succinate and succinate receptor (GPR91) signaling pathway are involved in the activation, proliferation, and migration of HSCs. We investigated whether succinate may induce hepatic fibrosis. The mice were randomly divided into 2 groups —the control group (chow diet-fed mice, n = 26) and sodium succinate group (2% sodium succinate + chow diet, n = 38). Each diet was provided for 16 weeks. Mice administered an oral diet of 2% sodium succinate for sixteen weeks lost body weight and had increased serum alanine transaminase and hepatic triglyceride contents compared to those in the control mice. Moreover, mice fed with sodium succinate showed increased expression of the alpha smooth muscle actin protein and gene in the liver at 8 weeks of feeding and increased fibrosis in their histology at 16 weeks of feeding. However, the expression of the GPR91 protein and mRNA increased at 4 weeks of feeding, but decreased at 8 and 16 weeks of feeding. These results suggest that an oral succinate diet could induce liver damage and liver fibrosis in mice and that GPR91 signaling might be an early marker or sensor of hepatic fibrosis development.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133416064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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