Recent evidence suggests obesity as a low or systemic chronic inflammation. (Pre) adipocytes in the adipose tissue (AT) express and secrete a variety of cytokines and adipokines. Inducible nitric oxide synthase (iNOS) is an inflammatory enzyme involved in the production of NO. Until now, the inducer(s) of iNOS expression in (pre)adipocytes remains unclear. In this study, we investigated the effects of proinflammatory cytokines [interleukin-1β (IL-1β), IL-10, IL-12, interferon-γ (IFN-γ)], adipokines [retinol-binding protein 4 (RBP4), adiponectin, leptin, and resistin], and lipopolysaccharide (LPS), a bacterial cell wall component, on the expression of iNOS in 3T3-L1 preadipocytes. Notably, treatment with IL-1β at 20 ng/mL for 4 h markedly increased iNOS mRNA expression in 3T3-L1 preadipocytes, but that with IL10 (10 ng/mL), IL-12 (5 ng/mL), IFN-γ (10 ng/mL), RBP4 (5 ug/mL), adiponectin (100 ng/mL), leptin (100 ng/mL), and resistin (100 ng/mL), and LPS (1 ug/mL) for 4 h had little or no effect on it. Results of dose-response and time-course experiments confirmed the ability of IL-1β at 20 ng/mL for 4 h to maximally induce iNOS mRNA expression in 3T3-L1 preadipocytes. Importantly, pharmacological inhibition studies demonstrated that treatment with AG490 [an inhibitor of Janus-activated kinases (JAKs) and signal transducer and activator of transcription proteins (STATs)], GO6976 (an inhibitor of PKCs), or PP1 (an Src kinase inhibitor) suppressed IL-1βinduced iNOS mRNA expression in 3T3-L1 preadipocytes, pointing out the involvement of JAKs/STATs, PKCs, and Src in the process. This work advocates that IL-1β is a major and strong inducer of iNOS expression in 3T3-L1 preadipocytes.
{"title":"Interleukin 1β Up-Regulates mRNA Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes: Role of JAKs/STATs, PKCs, and Src","authors":"Yu-Kyoung Park, Sai-zhen Wang, Byeong-Churl Jang","doi":"10.46308/KMJ.2021.00108","DOIUrl":"https://doi.org/10.46308/KMJ.2021.00108","url":null,"abstract":"Recent evidence suggests obesity as a low or systemic chronic inflammation. (Pre) adipocytes in the adipose tissue (AT) express and secrete a variety of cytokines and adipokines. Inducible nitric oxide synthase (iNOS) is an inflammatory enzyme involved in the production of NO. Until now, the inducer(s) of iNOS expression in (pre)adipocytes remains unclear. In this study, we investigated the effects of proinflammatory cytokines [interleukin-1β (IL-1β), IL-10, IL-12, interferon-γ (IFN-γ)], adipokines [retinol-binding protein 4 (RBP4), adiponectin, leptin, and resistin], and lipopolysaccharide (LPS), a bacterial cell wall component, on the expression of iNOS in 3T3-L1 preadipocytes. Notably, treatment with IL-1β at 20 ng/mL for 4 h markedly increased iNOS mRNA expression in 3T3-L1 preadipocytes, but that with IL10 (10 ng/mL), IL-12 (5 ng/mL), IFN-γ (10 ng/mL), RBP4 (5 ug/mL), adiponectin (100 ng/mL), leptin (100 ng/mL), and resistin (100 ng/mL), and LPS (1 ug/mL) for 4 h had little or no effect on it. Results of dose-response and time-course experiments confirmed the ability of IL-1β at 20 ng/mL for 4 h to maximally induce iNOS mRNA expression in 3T3-L1 preadipocytes. Importantly, pharmacological inhibition studies demonstrated that treatment with AG490 [an inhibitor of Janus-activated kinases (JAKs) and signal transducer and activator of transcription proteins (STATs)], GO6976 (an inhibitor of PKCs), or PP1 (an Src kinase inhibitor) suppressed IL-1βinduced iNOS mRNA expression in 3T3-L1 preadipocytes, pointing out the involvement of JAKs/STATs, PKCs, and Src in the process. This work advocates that IL-1β is a major and strong inducer of iNOS expression in 3T3-L1 preadipocytes.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131779023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiseob Kim, Hyo-jin Lee, N. Ha, In-Joong Kim, K. Park
Lower back pain in pregnant women is common and 30% of them require bed rest as a result [1]. Meanwhile, sciatica associated intractable pain occurs in approximately 1:10,000 pregnant women [1]. The occurrence of lower back pain and its severity are affected by hormone changes, weight gain, shift in center of gravity leading to disc bulging or herniation and relaxin levels, which contribute to the sliding mobility of the sacroiliac joints, widening of the pubic symphysis, and laxity of the posterior longitudinal ligament [2]. Conservative therapy, such as stretching, acupuncture, and core training, should be tried as first line interventions and analgesics, such as nonsteroidal anti-inflammatory drugs, may be recommended following the risk classification guidelines for drug use in pregnancy. In non-pregnant patients, disc length is a predictor for surgical treatment or nerve block success [3]. However, there are no definitive guidelines for nerve block and drug combinations in pregnant women with sciatica or lower back pain. This case report aimed to investigate the efficacy of epidural catheter insertion and intermittent local analgesic administration in a pregnant woman, presenting with severe, acute lower back pain and sciatica.
{"title":"Treatment of Low Back Pain and Sciatica by Local Anesthetic Injection through Epidural Catheter in Pregnant Woman","authors":"Jiseob Kim, Hyo-jin Lee, N. Ha, In-Joong Kim, K. Park","doi":"10.46308/KMJ.2021.00059","DOIUrl":"https://doi.org/10.46308/KMJ.2021.00059","url":null,"abstract":"Lower back pain in pregnant women is common and 30% of them require bed rest as a result [1]. Meanwhile, sciatica associated intractable pain occurs in approximately 1:10,000 pregnant women [1]. The occurrence of lower back pain and its severity are affected by hormone changes, weight gain, shift in center of gravity leading to disc bulging or herniation and relaxin levels, which contribute to the sliding mobility of the sacroiliac joints, widening of the pubic symphysis, and laxity of the posterior longitudinal ligament [2]. Conservative therapy, such as stretching, acupuncture, and core training, should be tried as first line interventions and analgesics, such as nonsteroidal anti-inflammatory drugs, may be recommended following the risk classification guidelines for drug use in pregnancy. In non-pregnant patients, disc length is a predictor for surgical treatment or nerve block success [3]. However, there are no definitive guidelines for nerve block and drug combinations in pregnant women with sciatica or lower back pain. This case report aimed to investigate the efficacy of epidural catheter insertion and intermittent local analgesic administration in a pregnant woman, presenting with severe, acute lower back pain and sciatica.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116256584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hong, Ji Yeon Lee, Miri Hyun, Jae Seok Park, Jae Hyuck Lee, Y. Suh, H. Kim, D. Kim
About 30~50% coronavirus disease 2019 (COVID-19) hospital inpatients is report-ed to have mild clinical presentation
据报道，约30~50%的新冠肺炎住院患者临床表现较轻
{"title":"Clinical Characteristics of Mild Coronavirus Disease 2019 Inpatients","authors":"S. Hong, Ji Yeon Lee, Miri Hyun, Jae Seok Park, Jae Hyuck Lee, Y. Suh, H. Kim, D. Kim","doi":"10.46308/kmj.2020.00192","DOIUrl":"https://doi.org/10.46308/kmj.2020.00192","url":null,"abstract":"About 30~50% coronavirus disease 2019 (COVID-19) hospital inpatients is report-ed to have mild clinical presentation","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124169478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In 2019, a novel betacoronavirus, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), was detected in the Chinese city of Wuhan. SARS-CoV-2 caused a severe form of pneumonias which is closely related to SARS-CoV-1. Detection of viral RNA by real-time reverse transcription polymerase reaction (real-time RT-PCR) is crucial for the management of the patients and prevention of the SARS-CoV-2. Until now real-time RT-PCR methods have been used as a successful diagnostic tool in this epidemic. Furthrmore, more rapid and reliable diagnostic tool might be needed for the next epidemic.
{"title":"Understandings and Prospects of Laboratory Diagnosis of SARS-CoV-2","authors":"Jae-Seok Kim","doi":"10.46308/kmj.2020.00199","DOIUrl":"https://doi.org/10.46308/kmj.2020.00199","url":null,"abstract":"In 2019, a novel betacoronavirus, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), was detected in the Chinese city of Wuhan. SARS-CoV-2 caused a severe form of pneumonias which is closely related to SARS-CoV-1. Detection of viral RNA by real-time reverse transcription polymerase reaction (real-time RT-PCR) is crucial for the management of the patients and prevention of the SARS-CoV-2. Until now real-time RT-PCR methods have been used as a successful diagnostic tool in this epidemic. Furthrmore, more rapid and reliable diagnostic tool might be needed for the next epidemic.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129986237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was conducted to study the job interest and job value of medical freshmen. The job interest test and job value test by the Korea Employment Information Service of the Ministry of Employment and Labor were conducted on 149 medical freshmen enrolled in a medical school at 2019 and 2020. Among six types of job interest, investigative type was most suitable for the medical freshmen, followed by realistic type, enterprising type, social type. Among 13 types of job value, job stability, body and mind space, recognition, and financial compensation were higher values than the others in order. We expect that this result will be helpful to establish the career identity of medical students, and to construct the system of curriculum or counseling programs for career developing of them.
{"title":"Analysis of Job Interest and Job Value in the Freshmen of a Medical School","authors":"Hye‐Jin Park, W. Park","doi":"10.46308/kmj.2020.00185","DOIUrl":"https://doi.org/10.46308/kmj.2020.00185","url":null,"abstract":"This study was conducted to study the job interest and job value of medical freshmen. The job interest test and job value test by the Korea Employment Information Service of the Ministry of Employment and Labor were conducted on 149 medical freshmen enrolled in a medical school at 2019 and 2020. Among six types of job interest, investigative type was most suitable for the medical freshmen, followed by realistic type, enterprising type, social type. Among 13 types of job value, job stability, body and mind space, recognition, and financial compensation were higher values than the others in order. We expect that this result will be helpful to establish the career identity of medical students, and to construct the system of curriculum or counseling programs for career developing of them.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"278 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124471909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Moon Sik Jung, Hyunho Lee, Hochang Kim, S. Park, Jimi Oh
Masseter muscle rigidity (MMR), known as the ‘jaw of steel’, sometimes makes laryngoscope unusable due to the stiffness of the jaw. MMR during general anesthesia was previously considered as an early episode of malignant hyperthermia (MH). Generally, MMR occurs in the pediatric patients after induction with succinylcholine and volatile agents. However, MMR following the administration of non-depolarizing muscle relaxants is uncommon, although described in some reports. We report a case of 62-year-old male patient which developed MMR following intravenous administration of rocuronium during induction of anesthesia. Our patient was not found to be vulnerable to MH. Although fiberoptic nasotracheal intubation had been successfully performed in our case, an unanticipated difficult airway due to MMR can be experienced at any time. Therefore, we should be aware that, although rare, MMR can occur after using non-depolarizing muscle relaxant. Additionally, in case of any incident of MMR, anesthesiologists need to recognize the management of a difficult airway and to maintain adequate oxygenation in a variety of methods according to a difficult airway algorithm.
{"title":"Rocuronium Induced Masseter Muscle Rigidity during Intubation in Patients with Dental Surgery","authors":"Moon Sik Jung, Hyunho Lee, Hochang Kim, S. Park, Jimi Oh","doi":"10.46308/kmj.2020.00143","DOIUrl":"https://doi.org/10.46308/kmj.2020.00143","url":null,"abstract":"Masseter muscle rigidity (MMR), known as the ‘jaw of steel’, sometimes makes laryngoscope unusable due to the stiffness of the jaw. MMR during general anesthesia was previously considered as an early episode of malignant hyperthermia (MH). Generally, MMR occurs in the pediatric patients after induction with succinylcholine and volatile agents. However, MMR following the administration of non-depolarizing muscle relaxants is uncommon, although described in some reports. We report a case of 62-year-old male patient which developed MMR following intravenous administration of rocuronium during induction of anesthesia. Our patient was not found to be vulnerable to MH. Although fiberoptic nasotracheal intubation had been successfully performed in our case, an unanticipated difficult airway due to MMR can be experienced at any time. Therefore, we should be aware that, although rare, MMR can occur after using non-depolarizing muscle relaxant. Additionally, in case of any incident of MMR, anesthesiologists need to recognize the management of a difficult airway and to maintain adequate oxygenation in a variety of methods according to a difficult airway algorithm.","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121343483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Computed Tomography in Evaluation of Femoral Anteversion: Transverse Section Versus Axial Oblique Section","authors":"Jong-Hyuk Jeon, S. Lee, K. Song, K. Bae","doi":"10.46308/kmj.2020.00045","DOIUrl":"https://doi.org/10.46308/kmj.2020.00045","url":null,"abstract":"Original","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133491833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
http://www.e-kmj.org Ruxolitinib for Treating Steroid-Refractory Acute Graft-Versus-Host Disease in an Infant with Malignant Osteopetrosis Who Received Double-Unit Umbilical Cord Blood Transplantation Jie Hee Jue, Ye Jee Shim, Sung-Ae Kim, Hye Ra Jung Department of Pediatrics, Keimyung University School of Medicine, Daegu, Korea Department of Dermatology, Keimyung University School of Medicine, Daegu, Korea Department of Pathology, Keimyung University School of Medicine, Daegu, Korea
http://www.e-kmj.org Ruxolitinib治疗接受双单位脐带血移植的恶性骨质硬化婴儿的类固醇难治性急性移植物抗宿主病Jie Hee Jue, Ye Jee Shim, Sung-Ae Kim, Hye Ra Jung大邱启明大学医学院儿科学，韩国大邱启明大学医学院皮肤病学，韩国大邱启明大学医学院病理学，韩国大邱
{"title":"Ruxolitinib for Treating Steroid-Refractory Acute Graft-Versus-Host Disease in an Infant with Malignant Osteopetrosis Who Received Double-Unit Umbilical Cord Blood Transplantation","authors":"Jie Hee Jue, Y. Shim, Sung-Ae Kim, H. Jung","doi":"10.46308/kmj.2021.00115","DOIUrl":"https://doi.org/10.46308/kmj.2021.00115","url":null,"abstract":"http://www.e-kmj.org Ruxolitinib for Treating Steroid-Refractory Acute Graft-Versus-Host Disease in an Infant with Malignant Osteopetrosis Who Received Double-Unit Umbilical Cord Blood Transplantation Jie Hee Jue, Ye Jee Shim, Sung-Ae Kim, Hye Ra Jung Department of Pediatrics, Keimyung University School of Medicine, Daegu, Korea Department of Dermatology, Keimyung University School of Medicine, Daegu, Korea Department of Pathology, Keimyung University School of Medicine, Daegu, Korea","PeriodicalId":166951,"journal":{"name":"Keimyung Medical Journal","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116573522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：