Journal of periodontal research最新文献_第9页

Progression from healthy periodontium to gingivitis and periodontitis: Insights from bioinformatics-driven proteomics – A systematic review with meta-analysis 从健康牙周到牙龈炎和牙周炎的进展：生物信息学驱动的蛋白质组学的启示--系统回顾与荟萃分析。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-14 DOI: 10.1111/jre.13313

Paras Ahmad, Andrea Escalante-Herrera, Lina M. Marin, Walter L. Siqueira

Aim

The current study aimed to: (1) systematically review the published literature regarding the proteomics analyses of saliva and gingival crevicular fluid (GCF) in healthy humans and gingivitis and/or periodontitis patients; and (2) to identify the differentially expressed proteins (DEPs) based on the systematic review, and comprehensively conduct meta-analyses and bioinformatics analyses.

Methods

An online search of Web of Science, Scopus, and PubMed was performed without any restriction on the year and language of publication. After the identification of the DEPs reported by the included human primary studies, gene ontology (GO), the Kyoto encyclopedia of genes and genomes pathway (KEGG), protein–protein interaction (PPI), and meta-analyses were conducted. The risk of bias among the included studies was evaluated using the modified Newcastle–Ottawa quality assessment scale.

Results

The review identified significant differences in protein expression between healthy individuals and those with gingivitis and periodontitis. In GCF, 247 proteins were upregulated and 128 downregulated in periodontal diseases. Saliva analysis revealed 79 upregulated and 70 downregulated proteins. There were distinct protein profiles between gingivitis and periodontitis, with 159 and 31 unique upregulated proteins in GCF, respectively. Meta-analyses confirmed significant upregulation of various proteins in periodontitis, including ALB and MMP9, while CSTB and GSTP1 were downregulated. AMY1A and SERPINA1 were upregulated in periodontitis saliva. HBD was upregulated in gingivitis GCF, while DEFA3 was downregulated. PPI analysis revealed complex networks of interactions among DEPs. GO and KEGG pathway analyses provided insights into biological processes and pathways associated with periodontal diseases.

Conclusion

The ongoing MS-based proteomics studies emphasize the need for a highly sensitive and specific diagnostic tool for periodontal diseases. Clinician acceptance of the eventual diagnostic method relies on its ability to provide superior or complementary information to current clinical assessment procedures. Future research should prioritize the multiplex measurement of multiple biomarkers simultaneously to enhance diagnostic accuracy and large study cohorts are necessary to ensure the validity and reliability of research findings.

目的：本研究旨在(1）系统回顾已发表的有关健康人和牙龈炎及/或牙周炎患者唾液和牙龈缝隙液（GCF）蛋白质组学分析的文献；（2）在系统回顾的基础上识别差异表达蛋白质（DEPs），并全面进行荟萃分析和生物信息学分析：方法：对 Web of Science、Scopus 和 PubMed 进行在线检索，不限制发表年份和语言。在确定了纳入的人类主要研究报告中的 DEPs 后，进行了基因本体（GO）、京都基因和基因组途径百科全书（KEGG）、蛋白质-蛋白质相互作用（PPI）和荟萃分析。采用修改后的纽卡斯尔-渥太华质量评估量表对纳入研究的偏倚风险进行了评估：研究发现，健康人与牙龈炎和牙周炎患者的蛋白质表达存在明显差异。在 GCF 中，有 247 种蛋白质上调，128 种蛋白质下调。唾液分析显示有 79 种蛋白质上调，70 种蛋白质下调。牙龈炎和牙周炎的蛋白质特征截然不同，牙龈纤维中分别有159种和31种独特的上调蛋白质。Meta 分析证实，牙周炎患者的多种蛋白质（包括 ALB 和 MMP9）显著上调，而 CSTB 和 GSTP1 则下调。AMY1A和SERPINA1在牙周炎唾液中上调。HBD 在牙龈炎 GCF 中上调，而 DEFA3 则下调。PPI分析揭示了DEPs之间复杂的相互作用网络。GO和KEGG通路分析深入揭示了与牙周疾病相关的生物过程和通路：正在进行的基于 MS 的蛋白质组学研究强调，需要一种高灵敏度和特异性的牙周疾病诊断工具。临床医生对最终诊断方法的接受程度取决于它能否提供优于或补充当前临床评估程序的信息。未来的研究应优先考虑同时对多种生物标记物进行多重测量，以提高诊断的准确性，同时有必要进行大规模的队列研究，以确保研究结果的有效性和可靠性。

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引用次数: 0

Association between asthma and periodontitis: A case–control analysis of risk factors, related medications, and allergic responses 哮喘与牙周炎之间的关系：对风险因素、相关药物和过敏反应的病例对照分析。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-10 DOI: 10.1111/jre.13311

Muhammad H. A. Saleh, Ann M. Decker, Khushboo Kalani, Khoa Hoang, Obada Mandil, Parth Gathalia, Bidisha Ray, Njira Lugogo, Hom-Lay Wang

Aims

This study aimed to investigate the association between asthma, related allergies and medication use, and the presence and severity of periodontitis among individuals at the University of Michigan School of Dentistry.

Methods

Employing a case–control design, the study analyzed data from 892 patients, half with asthma and half without asthma. Data collection included demographics, asthma history, medication use, allergies, and periodontal examination outcomes, including probing pocket depth (PPD), mobility, furcation involvement, and radiographic bone loss (RBL). Logistic regression models assessed the relationship between asthma and periodontitis, adjusting for confounders.

Results

Asthmatic patients exhibited significantly lower odds of periodontitis (OR = 0.10, p < .001) and were less likely to present with advanced stages (OR = 0.23, p < .001) and grades of the disease (OR = 0.31, p < .001) compared to non-asthmatic patients. The study also found a higher proportion of females in the asthmatic group (67% vs. 51.8%, p < .001). Smoking was identified as a significant factor associated with periodontitis in patients with asthma, with former smokers at more than double the odds (OR = 2.28, p = .035) and current smokers at a slightly lower yet significant odds (OR = 1.87, p = .050). Additionally, asthmatic patients on adrenergic inhalers had an increased likelihood of developing periodontitis (OR = 1.76, p = .045). Allergies to codeine and latex were associated with higher odds of periodontitis, with ORs of 3.41 and 6.09, respectively.

Conclusions

Asthma was found to be associated with lower odds of periodontitis. However, this association appears to be modified by smoking habits and the use of certain asthma medications, which are related to an increased likelihood of periodontitis among asthmatic patients.

目的：本研究旨在调查密歇根大学牙科学院的哮喘、相关过敏症和药物使用与牙周炎的存在和严重程度之间的关系：研究采用病例对照设计，分析了 892 名患者的数据，其中一半患有哮喘，一半没有哮喘。收集的数据包括人口统计学特征、哮喘病史、用药情况、过敏史和牙周检查结果，包括探诊袋深度（PPD）、活动度、毛囊受累情况和放射骨质流失（RBL）。逻辑回归模型评估了哮喘与牙周炎之间的关系，并对混杂因素进行了调整：结果：哮喘病人患牙周炎的几率明显较低（OR = 0.10，p 结论：哮喘与牙周炎的关系与哮喘病人的哮喘程度有关：哮喘与牙周炎发生几率较低有关。然而，吸烟习惯和使用某些哮喘药物似乎会改变这种关联，因为这与哮喘患者患牙周炎的可能性增加有关。

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引用次数: 0

Recombinant human fibroblast growth factor and autogenous bone for periodontal regeneration: Alone or in combination? A randomized clinical trial 重组人成纤维细胞生长因子和自体骨用于牙周再生：单独使用还是联合使用？随机临床试验。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-09 DOI: 10.1111/jre.13310

Kosuke Kojima, Yohei Kamata, Tomoko Shimizu, Satsuki Sato, Sota Suzuki, Yuya Takanashi, Sawako Hojo, Takeshi Yoshino, Shinya Fuchida, Toshiyuki Tamura, Masato Minabe, Toshiro Kodama, Takaomi Kessoku, Shunsuke Oyamada

Aim

To compare the outcomes of therapy using recombinant human fibroblast growth factor (rhFGF)-2 combined with autologous bone grafting (ABG) therapy with those of rhFGF-2 alone and ABG alone in the treatment of periodontal intraosseous defects.

Methods

Periodontal intraosseous defects were randomized to receive rhFGF-2 therapy + ABG, rhFGF-2 therapy alone, or ABG alone. Periodontal examination and periapical radiography were performed preoperatively and at 3, 6, and 12 months postoperatively.

Results

At the 12 months follow-up, all three groups showed significant improvement in the clinical attachment level (CAL): 5.6 ± 1.6, 5.8 ± 1.7, and 5.2 ± 1.6 mm in the rhFGF-2 + ABG, rhFGF-2 alone, and ABG alone groups, respectively, with no significant inter-group differences (p < .05). rhFGF-2 therapy (alone or in combination) resulted in greater bone defect filling (BDF) (2.3 ± 1.2 mm and 2.6 ± 1.9 mm, respectively) than ABG therapy alone (1.2 ± 1.2 mm). Gingival recession was lesser in the ABG alone (1.2 ± 1.1 mm) and rhFGF-2 + ABG groups (1.4 ± 0.8 mm) than in the rhFGF-2 alone group (2.2 ± 1.2 mm).

Conclusion

The results of this study showed that at 12 months postoperatively, all treatments resulted in statistically significant clinical improvements compared to the baseline. From these results, it can be concluded that rhFGF-2 promotes hard tissue regeneration in intraosseous defects.

目的：比较使用重组人成纤维细胞生长因子（rhFGF）-2联合自体骨移植（ABG）疗法与单独使用rhFGF-2疗法和单独使用ABG疗法治疗牙周骨内缺损的效果：方法：将牙周骨内缺损患者随机分为接受 rhFGF-2 + ABG 治疗、单独接受 rhFGF-2 治疗或单独接受 ABG 治疗。术前、术后 3 个月、6 个月和 12 个月分别进行牙周检查和根尖周放射摄影：随访 12 个月时，三组患者的临床附着水平（CAL）均有显著改善：rhFGF-2 + ABG 组、单纯 rhFGF-2 组和单纯 ABG 组的临床附着水平（CAL）分别为 5.6 ± 1.6、5.8 ± 1.7 和 5.2 ± 1.6 mm，组间差异不显著（p 结论：该研究结果表明，术后 12 个月时，rhFGF-2 + ABG 组、单纯 rhFGF-2 组和单纯 ABG 组的临床附着水平（CAL）均有显著改善：本研究结果表明，术后 12 个月时，所有治疗方法都能使临床症状较基线有明显的统计学改善。由此可以得出结论，rhFGF-2 能促进骨内缺损的硬组织再生。

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引用次数: 0

Low-energy red light-emitting diode irradiation enhances osteogenic differentiation of periodontal ligament stem cells by regulating miR-146a-5p 低能量红色发光二极管照射通过调节 miR-146a-5p 增强牙周韧带干细胞的成骨分化。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-06 DOI: 10.1111/jre.13276

Yajiao Ren, Shifen Wang, Hao Li, Jiaxin Li, Xiaorong Lan, Yao Wang

Aims

The study aimed to investigate the role of miR-146a-5p in osteogenesis of hPDLSCs irradiated with low-energy red LEDs.

Methods

After irradiation with 5 J/cm² red LED, miR-146a-5p expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and osteogenic markers expression was determined by RT-qPCR and Western blotting. Alkaline phosphatase (ALP) activity was assessed by ALP staining, and mineralization was assessed by Alizarin Red staining, respectively. Lentiviral vectors were designed to regulate miR-146a-5p expression. Dual-luciferase reporter assay was performed to confirm the targeted relationship between miR-146a-5p and MAPK1. Short hairpin RNA (shRNA) was used to regulate MAPK1 expression.

Results

RT-qPCR and western blotting revealed that 5 J/cm² irradiation elevated the levels of the osteogenic markers osterix (OSX) and bone sialoprotein (BSP) in hPDLSCs. miR-146a-5p is downregulated in hPDLSCs under the low-energy red LED light irradiation. miR-146a-5p underexpression markedly promoted the osteogenic potential of hPDLSCs. miR-146a-5p targeted MAPK1. 5 J/cm² red LED irradiation rescued the inhibitory effects of upregulated miR-146a-5p on osteogenic differentiation, and the positive influence of red LED irradiation could be reversed by downregulated MAPK1.

Conclusion

These findings confirm that miR-146a-5p is involved in the effect of LED irradiation on the osteogenic differentiation of hPDLSCs by targeting MAPK1. Red LED irradiation may be a potential clinical adjunct therapy for periodontal regeneration.

目的：本研究旨在探讨miR-146a-5p在低能量红光LED照射hPDLSCs成骨过程中的作用：方法：用 5 J/cm2 红色 LED 照射 hPDLSCs 后，通过实时定量聚合酶链反应（RT-qPCR）检测 miR-146a-5p 的表达，并通过 RT-qPCR 和 Western 印迹检测成骨标志物的表达。碱性磷酸酶（ALP）活性通过ALP染色评估，矿化度通过茜素红染色评估。设计了慢病毒载体来调控 miR-146a-5p 的表达。双荧光素酶报告实验证实了 miR-146a-5p 与 MAPK1 之间的靶向关系。用短发夹RNA（shRNA）调控MAPK1的表达：miR-146a-5p靶向MAPK1。5焦耳/平方厘米的红色LED照射可挽救上调的miR-146a-5p对成骨分化的抑制作用，红色LED照射的积极影响可被下调的MAPK1逆转：这些研究结果证实，miR-146a-5p通过靶向MAPK1参与了LED照射对hPDLSCs成骨分化的影响。红光LED照射可能是一种潜在的牙周再生临床辅助疗法。

{"title":"Low-energy red light-emitting diode irradiation enhances osteogenic differentiation of periodontal ligament stem cells by regulating miR-146a-5p","authors":"Yajiao Ren, Shifen Wang, Hao Li, Jiaxin Li, Xiaorong Lan, Yao Wang","doi":"10.1111/jre.13276","DOIUrl":"10.1111/jre.13276","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>The study aimed to investigate the role of miR-146a-5p in osteogenesis of hPDLSCs irradiated with low-energy red LEDs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>After irradiation with 5 J/cm<sup>2</sup> red LED, miR-146a-5p expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and osteogenic markers expression was determined by RT-qPCR and Western blotting. Alkaline phosphatase (ALP) activity was assessed by ALP staining, and mineralization was assessed by Alizarin Red staining, respectively. Lentiviral vectors were designed to regulate miR-146a-5p expression. Dual-luciferase reporter assay was performed to confirm the targeted relationship between miR-146a-5p and MAPK1. Short hairpin RNA (shRNA) was used to regulate MAPK1 expression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>RT-qPCR and western blotting revealed that 5 J/cm<sup>2</sup> irradiation elevated the levels of the osteogenic markers osterix (OSX) and bone sialoprotein (BSP) in hPDLSCs. miR-146a-5p is downregulated in hPDLSCs under the low-energy red LED light irradiation. miR-146a-5p underexpression markedly promoted the osteogenic potential of hPDLSCs. miR-146a-5p targeted MAPK1. 5 J/cm<sup>2</sup> red LED irradiation rescued the inhibitory effects of upregulated miR-146a-5p on osteogenic differentiation, and the positive influence of red LED irradiation could be reversed by downregulated MAPK1.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings confirm that miR-146a-5p is involved in the effect of LED irradiation on the osteogenic differentiation of hPDLSCs by targeting MAPK1. Red LED irradiation may be a potential clinical adjunct therapy for periodontal regeneration.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"59 5","pages":"1031-1041"},"PeriodicalIF":3.4,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soft tissue elasticity at teeth and implant sites. A novel outcome measure of the soft tissue phenotype 牙齿和种植部位的软组织弹性。衡量软组织表型的新成果。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-05 DOI: 10.1111/jre.13296

Lorenzo Tavelli, Shayan Barootchi

<div> <section> <h3> Aim</h3> <p>To assess ultrasonographic tissue elasticity at teeth and implant sites and its variation after peri-implant soft tissue augmentation with a connective tissue graft (CTG).</p> </section> <section> <h3> Methods</h3> <p>Twenty-eight patients, each contributing with one clinically healthy dental implant exhibiting a soft tissue dehiscence (PSTD), were included. Implant sites were augmented with CTG and monitored over 12 months. Ultrasonographic strain elastography, expressed as strain ratios (SR<sub>1</sub>, SR<sub>2</sub>, and SR<sub>3</sub>, respectively) was assessed at baseline, 6-, and 12-month, and compared with the corresponding contralateral homologous natural tooth. SR<sub>1</sub> assessed the strain/elasticity of the midfacial coronal portion of the soft tissue in comparison to the natural tooth crown/implant-supported crown, SR<sub>2</sub> evaluated the strain of the midfacial coronal soft tissue in relation to the one of the alveolar mucosa, while SR<sub>3</sub> depicted the strain of the midfacial soft tissue in relation to the interproximal soft tissue on the transverse ultrasound scan.</p> </section> <section> <h3> Results</h3> <p>SR<sub>1</sub> in natural dentition and at implant sites was 0.20 ± 0.08 and 0.30 ± 0.14, respectively (<i>p</i> = .002), indicating that the coronal portion of the soft tissue around teeth is generally more elastic than its counterpart around dental implants. Soft tissue augmentation with CTG promoted an increased stiffness of the midfacial coronal portion of the soft tissue over 12 months (<i>p</i> < .001 for SR<sub>1</sub>, SR<sub>2</sub>, and SR<sub>3</sub>). Strain ratios at the 12-month time points were significantly higher than the values observed at 6 months (<i>p</i> < .001). Regression analysis demonstrated that strain elastography ratios in natural dentition were significantly associated with keratinized gingiva width, and gingival thickness. At implant sites, SR<sub>1</sub> was significantly associated with keratinized mucosa width and mucosal thickness (<i>p</i> < .001 for both correlations), SR<sub>2</sub> was significantly associated with keratinized mucosa width (<i>p</i> = .013), and SR3 was significantly associated with the surgical technique performed in combination with CTG (<i>p</i> = .022).</p> </section> <section> <h3> Conclusion</h3> <p>Ultrasound strain elastography captures and quantifies tissue elasticity and its changes after soft tissue augmentation. A different baseline tissue elasticity was observed between teeth and dental implants in th

目的：评估使用结缔组织移植（CTG）增强种植体周围软组织后，牙齿和种植体部位的超声波组织弹性及其变化：共纳入 28 名患者，每名患者都有一颗临床健康的牙种植体出现软组织开裂 (PSTD)。使用 CTG 增加种植部位，并对其进行为期 12 个月的监测。分别在基线、6 个月和 12 个月时对超声应变弹性成像（以应变比（SR1、SR2 和 SR3）表示）进行评估，并与相应的对侧同源天然牙进行比较。SR1 评估的是与天然牙冠/种植体支持的牙冠相比，面中部冠状部分软组织的应变/弹性；SR2 评估的是与牙槽粘膜相比，面中部冠状部分软组织的应变；而 SR3 描述的是在横向超声扫描中，面中部软组织与近端间软组织相比的应变：天然牙和种植体部位的 SR1 分别为 0.20 ± 0.08 和 0.30 ± 0.14（p = .002），表明牙齿周围软组织的冠状部分通常比种植体周围的软组织更具弹性。使用 CTG 进行软组织增量可在 12 个月内提高软组织中面部冠状部分的硬度（p 1、SR2 和 SR3）。12 个月时间点的应变比明显高于 6 个月时的观察值（p 1 与角化粘膜宽度和粘膜厚度明显相关（p 2 与角化粘膜宽度明显相关（p = .013），SR3 与结合 CTG 进行的手术技术明显相关（p = .022））：结论：超声应变弹性成像可捕捉和量化组织弹性及其在软组织增量术后的变化。在软组织的最冠状面，观察到牙齿和牙种植体的基线组织弹性不同。影响组织弹性相关结果的主要因素是角化组织宽度和粘膜厚度。

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引用次数: 0

Bidirectional associations between periodontitis and inflammatory bowel disease: A systematic review of longitudinal studies with meta-analysis and trial sequential analysis 牙周炎与炎症性肠病之间的双向关联：通过荟萃分析和试验序列分析对纵向研究进行系统回顾。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-04 DOI: 10.1111/jre.13291

Qiuhao Wang, Shuze Chen, Jieyu Zhou, Lei Zhao

The bidirectional associations between periodontitis and inflammatory bowel disease (IBD) with temporal directionality remain inconclusive. This study aims to evaluate the bidirectional associations between periodontitis and IBD through a systematic review and meta-analysis. Five databases (PubMed, Embase, Web of Science, Scopus and Cochrane Library) were systematically searched from inception to 27 February 2024. Two independent reviewers performed a review of the retrieved studies. Longitudinal studies, including cohort and nested case–control studies, were considered eligible for the study design. The pooled risk ratio (RR) and hazard ratio (HR) derived from the meta-analysis were used to assess whether periodontitis (or IBD) was a risk factor for IBD (or periodontitis). Trial sequential analysis (TSA) was performed to evaluate the reliability of the results. Four studies (n = 10 270 912) on the risk of IBD in patients with periodontitis and two (n = 33 420) on the risk of periodontitis in patients with IBD were included. The result suggested that periodontitis did not increase the risk of IBD (pooled RR = 1.04, 95% confidence interval [CI]: 0.99–1.09; p = .164; I-squared statistic [I²] = 27%). For subtypes of IBD, periodontitis was associated with the occurrence of ulcerative colitis (UC) (pooled RR = 1.12, 95% CI: 1.04–1.21; p = .003; I² = 38%), but not with Crohn's disease (CD) (pooled RR = 0.98, 95% CI: 0.92–1.04; p = .475; I² = 0%). Specifically, the risk of UC was higher among men (pooled HR = 1.11, 95% CI: 1.01–1.22; p = .025; I² = 0%) and smokers (pooled HR = 1.23, 95% CI: 1.07–1.42; p = .004; I² = 0%) with periodontitis than their counterparts without periodontitis. Patients with IBD may have a higher risk of developing periodontitis (pooled HR = 1.37, 95% CI: 1.26–1.49; p < .001; I² = 18%); however, whether IBD subtypes increased the occurrence of periodontitis remained uncertain. The TSA results confirmed the reliability of the primary findings. Based on limited longitudinal evidence, patients with periodontitis do not exhibit an increased risk of developing IBD overall, but they are at increased risk of UC (not CD). On the contrary, patients with IBD have a higher risk of developing periodontitis over time. More high-quality longitudinal studies are needed to determine the effect of specific subtypes of IBD on periodontitis.

牙周炎与炎症性肠病（IBD）之间的双向关系在时间方向上仍无定论。本研究旨在通过系统综述和荟萃分析评估牙周炎与 IBD 之间的双向关联。研究人员对五个数据库（PubMed、Embase、Web of Science、Scopus 和 Cochrane Library）进行了系统检索，检索时间从开始到 2024 年 2 月 27 日。两名独立审查员对检索到的研究进行了审查。纵向研究（包括队列研究和嵌套病例对照研究）被认为符合研究设计的要求。荟萃分析得出的风险比（RR）和危险比（HR）用于评估牙周炎（或 IBD）是否是 IBD（或牙周炎）的风险因素。为评估结果的可靠性，进行了试验序列分析（TSA）。其中包括四项关于牙周炎患者罹患 IBD 风险的研究（n = 10 270 912）和两项关于 IBD 患者罹患牙周炎风险的研究（n = 33 420）。结果表明，牙周炎不会增加 IBD 的风险（汇总 RR = 1.04，95% 置信区间 [CI]：0.99-1.09；P=0.05）：0.99-1.09; p = .164; I-squared statistic [I2] = 27%）。就IBD亚型而言，牙周炎与溃疡性结肠炎（UC）的发生有关（汇总RR = 1.12，95% CI：1.04-1.21；p = .003；I2 = 38%），但与克罗恩病（CD）无关（汇总RR = 0.98，95% CI：0.92-1.04；p = .475；I2 = 0%）。具体而言，患有牙周炎的男性（汇总 HR = 1.11，95% CI：1.01-1.22；p = .025；I2 = 0%）和吸烟者（汇总 HR = 1.23，95% CI：1.07-1.42；p = .004；I2 = 0%）比没有牙周炎的男性和吸烟者患 UC 的风险更高。IBD 患者患牙周炎的风险可能更高（汇总 HR = 1.37，95% CI：1.26-1.49；P 2 = 18%）；但是，IBD 亚型是否会增加牙周炎的发生率仍不确定。TSA结果证实了主要研究结果的可靠性。根据有限的纵向证据，牙周炎患者总体上患 IBD 的风险并没有增加，但他们患 UC（非 CD）的风险增加了。相反，随着时间的推移，IBD 患者患牙周炎的风险更高。需要更多高质量的纵向研究来确定特定亚型的 IBD 对牙周炎的影响。

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引用次数: 0

METTL3 promotes the osteogenic differentiation of human periodontal ligament cells by increasing YAP activity via IGF2BP1 and YTHDF1-mediated m6A modification METTL3 通过 IGF2BP1 和 YTHDF1 介导的 m6A 修饰提高 YAP 活性，从而促进人类牙周韧带细胞的成骨分化。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-04 DOI: 10.1111/jre.13297

Xuefei Sun, Xiujiao Meng, Yu Piao, Shaojie Dong, Qianqian Dong

Aims

N6-Methyladenosine (m⁶A) has been confirmed to play a dynamic role in osteoporosis and bone metabolism. However, whether m⁶A is involved in the osteogenic differentiation of human periodontal ligament cells (hPDLCs) remains unclear. The present study aimed to verify the role of methyltransferase-like 3 (METTL3)-mediated m⁶A modification in the osteogenic differentiation of hPDLCs.

Methods

The METTL3, Runx2, Osx, and YAP mRNA expression was determined by qPCR. METTL3, RUNX2, OSX, YTHDF1, YAP, IGF2BP1, and eIF3a protein expression was measured by Western blotting and immunofluorescence assays. The levels of m⁶A modification were evaluated by methylated RNA immunoprecipitation (MeRIP) and dot blot analyses. MeRIP-seq and RNA-seq were used to screen potential candidate genes. Nucleic acid and protein interactions were detected by immunoprecipitation. Alizarin red staining was used to evaluate the osteogenic differentiation of hPDLCs. Gene transcription and promoter activities were assessed by luciferase reporter assays (n ≥ 3).

Results

The expression of METTL3 and m⁶A modifications increased synchronously with the osteogenic differentiation of hPDLCs (p = .0016). YAP was a candidate gene identified by MeRIP-seq and RNA-seq, and its mRNA and protein expression levels were simultaneously increased. METTL3 increased the m⁶A methylated IGF2BP1-mediated stability of YAP mRNA (p = .0037), which in turn promoted osteogenic differentiation (p = .0147). Furthermore, METTL3 increased the translation efficiency of YAP by recruiting YTHDF1 and eIF3a to the translation initiation complex (p = .0154), thereby promoting the osteogenic differentiation of hPDLCs (p = .0012).

Conclusion

Our study revealed that METTL3-initiated m⁶A mRNA methylation promotes osteogenic differentiation of hPDLCs by increasing IGF2BP1-mediated YAP mRNA stability and recruiting YTHDF1 and eIF3a to the translation initiation complex to increase YAP mRNA translation. Our findings reveal the mechanism of METTL3-mediated m⁶A modification during hPDLC osteogenesis, providing a potential therapeutic target for periodontitis and alveolar bone defects.

目的：N6-甲基腺苷（m6A）已被证实在骨质疏松症和骨代谢中发挥动态作用。然而，m6A是否参与人牙周韧带细胞（hPDLCs）的成骨分化仍不清楚。本研究旨在验证甲基转移酶样3（METTL3）介导的m6A修饰在hPDLCs成骨分化中的作用：方法：通过qPCR检测METTL3、Runx2、Osx和YAP mRNA的表达。METTL3、RUNX2、OSX、YTHDF1、YAP、IGF2BP1和eIF3a蛋白的表达通过Western印迹和免疫荧光测定。甲基化 RNA 免疫沉淀（MeRIP）和点印迹分析评估了 m6A 修饰的水平。MeRIP-seq 和 RNA-seq 被用来筛选潜在的候选基因。通过免疫沉淀检测核酸和蛋白质的相互作用。茜素红染色用于评估hPDLCs的成骨分化。基因转录和启动子活性通过荧光素酶报告实验进行评估（n ≥ 3）：结果：METTL3和m6A修饰的表达与hPDLCs的成骨分化同步增加（p = .0016）。YAP是MeRIP-seq和RNA-seq鉴定出的候选基因，其mRNA和蛋白表达水平同时升高。METTL3 增加了 YAP mRNA 的 m6A 甲基化 IGF2BP1 介导的稳定性（p = .0037），这反过来又促进了成骨分化（p = .0147）。此外，METTL3通过招募YTHDF1和eIF3a进入翻译起始复合物，提高了YAP的翻译效率（p = .0154），从而促进了hPDLCs的成骨分化（p = .0012）：我们的研究发现，METTL3引发的m6A mRNA甲基化通过增加IGF2BP1介导的YAP mRNA稳定性，并招募YTHDF1和eIF3a进入翻译起始复合物以增加YAP mRNA翻译，从而促进hPDLCs的成骨分化。我们的研究结果揭示了 METTL3 在 hPDLC 成骨过程中介导 m6A 修饰的机制，为牙周炎和牙槽骨缺损提供了一个潜在的治疗靶点。

{"title":"METTL3 promotes the osteogenic differentiation of human periodontal ligament cells by increasing YAP activity via IGF2BP1 and YTHDF1-mediated m6A modification","authors":"Xuefei Sun, Xiujiao Meng, Yu Piao, Shaojie Dong, Qianqian Dong","doi":"10.1111/jre.13297","DOIUrl":"10.1111/jre.13297","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>N6-Methyladenosine (m<sup>6</sup>A) has been confirmed to play a dynamic role in osteoporosis and bone metabolism. However, whether m<sup>6</sup>A is involved in the osteogenic differentiation of human periodontal ligament cells (hPDLCs) remains unclear. The present study aimed to verify the role of methyltransferase-like 3 (METTL3)-mediated m<sup>6</sup>A modification in the osteogenic differentiation of hPDLCs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The METTL3, Runx2, Osx, and YAP mRNA expression was determined by qPCR. METTL3, RUNX2, OSX, YTHDF1, YAP, IGF2BP1, and eIF3a protein expression was measured by Western blotting and immunofluorescence assays. The levels of m<sup>6</sup>A modification were evaluated by methylated RNA immunoprecipitation (MeRIP) and dot blot analyses. MeRIP-seq and RNA-seq were used to screen potential candidate genes. Nucleic acid and protein interactions were detected by immunoprecipitation. Alizarin red staining was used to evaluate the osteogenic differentiation of hPDLCs. Gene transcription and promoter activities were assessed by luciferase reporter assays (<i>n</i> ≥ 3).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The expression of METTL3 and m<sup>6</sup>A modifications increased synchronously with the osteogenic differentiation of hPDLCs (<i>p</i> = .0016). YAP was a candidate gene identified by MeRIP-seq and RNA-seq, and its mRNA and protein expression levels were simultaneously increased. METTL3 increased the m<sup>6</sup>A methylated IGF2BP1-mediated stability of YAP mRNA (<i>p</i> = .0037), which in turn promoted osteogenic differentiation (<i>p</i> = .0147). Furthermore, METTL3 increased the translation efficiency of YAP by recruiting YTHDF1 and eIF3a to the translation initiation complex (<i>p</i> = .0154), thereby promoting the osteogenic differentiation of hPDLCs (<i>p</i> = .0012).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study revealed that METTL3-initiated m<sup>6</sup>A mRNA methylation promotes osteogenic differentiation of hPDLCs by increasing IGF2BP1-mediated YAP mRNA stability and recruiting YTHDF1 and eIF3a to the translation initiation complex to increase YAP mRNA translation. Our findings reveal the mechanism of METTL3-mediated m<sup>6</sup>A modification during hPDLC osteogenesis, providing a potential therapeutic target for periodontitis and alveolar bone defects.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"59 5","pages":"1017-1030"},"PeriodicalIF":3.4,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cementum and enamel surface mimicry influences soft tissue cell behavior 牙本质和牙釉质表面模拟影响软组织细胞的行为。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-06-03 DOI: 10.1111/jre.13295

Benjamin Bellon, Benjamin Pippenger, Alexandra Stähli, Martin Degen, Ludovica Parisi

Aims

To test whether titanium surface roughness disparity might be used to specifically guide the behavior of gingiva fibroblasts and keratinocytes, thereby improving the quality of soft tissue (ST) integration around abutments.

Methods

Titanium discs resembling the roughness of enamel (M) or cementum (MA) were created with normal or increased hydrophilicity and used as substrates for human fibroblasts and keratinocytes. Adhesion and proliferation assays were performed to assess cell-type specific responses upon encountering the different surfaces. Additionally, immunofluorescence and qPCR analyses were performed to study more in depth the behavior of fibroblasts and keratinocytes on MA and M surfaces, respectively.

Results

While enamel-like M surfaces supported adhesion, growth and a normal differentiation potential of keratinocytes, cementum-emulating MA surfaces specifically impaired the growth of keratinocytes. Vice versa, MA surfaces sustained regular adhesion and proliferation of fibroblasts. Yet, a more intimate adhesion between fibroblasts and titanium was achieved by an increased hydrophilicity of MA surfaces, which was associated with an increased expression of elastin.

Conclusion

The optimal titanium implant abutment might be achieved by a bimodal roughness design, mimicking the roughness of enamel (M) and cementum with increased hydrophilicity (hMA), respectively. These surfaces can selectively elicit cell responses favoring proper ST barrier by impairing epithelial downgrowth and promoting firm adhesion of fibroblasts.

目的：测试钛表面粗糙度差异是否可用于特别引导牙龈成纤维细胞和角质形成细胞的行为，从而改善基台周围软组织（ST）整合的质量：方法：制作了与牙釉质（M）或牙胶结（MA）粗糙度相似的钛盘，亲水性正常或增加，用作人成纤维细胞和角质细胞的基底。进行了粘附和增殖试验，以评估细胞类型在遇到不同表面时的特异性反应。此外，还进行了免疫荧光和 qPCR 分析，以更深入地研究成纤维细胞和角质细胞分别在 MA 和 M 表面上的行为：结果：类珐琅质的 M 表面支持角质形成细胞的粘附、生长和正常分化潜能，而骨水泥质的 MA 表面则特别影响角质形成细胞的生长。反之亦然，MA 表面能维持成纤维细胞的正常粘附和增殖。然而，成纤维细胞与钛之间更紧密的粘附是通过增加 MA 表面的亲水性实现的，这与弹性蛋白表达的增加有关：结论：最佳的钛种植基台可通过双峰粗糙度设计来实现，即分别模仿釉质（M）和亲水性增加的骨水泥（hMA）的粗糙度。这些表面可以有选择性地引起细胞反应，通过影响上皮下生和促进成纤维细胞的牢固粘附来形成适当的 ST 屏障。

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引用次数: 0

Differences in maternal subgingival microbiome between preterm and term births: The MOHEPI study 早产儿和足月儿母体龈下微生物群的差异：MOHEPI 研究。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-05-29 DOI: 10.1111/jre.13292

Jung Soo Park, Eunha Kim, Sun Jae Kwon, Ju Sun Heo, Ki Hoon Ahn

Aim

Periodontitis is a potential risk factor for preterm birth (PTB) in women; however, the causal relationship or the exact mechanism remain unknown. This study aimed to compare the oral microbiome features of mothers with full-term birth (FTB) with those who had preterm delivery.

Methods

This study prospectively enrolled 60 women (30 mothers with PTB and 30 mothers with FTB), and subgingival plaque samples were collected and analysed by metagenomic 16S rDNA sequencing. Clinical measurements, including periodontal probing depth, clinical attachment level, modified gingival index (mGI) and plaque index, were performed to determine the periodontal state of the participants. Medical and obstetric data were collected as well.

Results

Among the periodontal measurements, mGI score, reflecting the level of gingival inflammation, exhibited a statistically significant association with PTB (adjusted odds ratio 2.705, 95% confidence interval 1.074–6.811, p = .035). When subgroup analysis was conducted based on mean mGI scores (mGI ≥ 2, high inflammation [HI] versus mGI < 2, low inflammation [LI]), microbiome analysis revealed clear distinctions in microbial compositions between PTB and FTB mothers in both the HI and LI groups. Especially in the HI group, alpha diversity exhibited a decreasing trend in PTB mothers compared to FTB mothers. Beta diversity also revealed significant differences between the two groups. In Linear Discriminant Analysis Effect Size analysis, certain anaerobic taxa, including the genera Spirochaetes, Treponema and Porphyromonas, were relatively abundant in the FTB/HI group, whereas the PTB/HI group showed a high abundance of the order Actinomycetales. Network analysis showed that the FTB/HI had relatively stronger connectivity in microbial composition than the PTB/HI group. Dysbiosis ratio of plaque microbiome, in terms of periodontitis, was significantly lower in PTB/HI group compared to FTB/HI group.

Conclusion

The compositions of maternal subgingival microbiomes differed between PTB and FTB mothers in both the high and low levels of gingival inflammation groups. In the presence of high level of gingival inflammation, dysbiosis in plaque microbiome, in terms of periodontitis, was decreased in PTB mothers compared to FTB mothers.

目的：牙周炎是女性早产（PTB）的潜在风险因素，但其因果关系或确切机制仍不清楚。本研究旨在比较足月产（FTB）母亲和早产母亲的口腔微生物组特征：本研究前瞻性地纳入了 60 名产妇（30 名早产产妇和 30 名足月产产妇），收集了龈下菌斑样本，并通过元基因组 16S rDNA 测序进行了分析。临床测量包括牙周探诊深度、临床附着水平、改良牙龈指数（mGI）和牙菌斑指数，以确定参与者的牙周状况。此外，还收集了医疗和产科数据：在牙周测量中，反映牙龈炎症程度的 mGI 评分与 PTB 有显著的统计学关联（调整后的几率比 2.705，95% 置信区间 1.074-6.811，p = .035）。如果根据 mGI 平均得分进行亚组分析（mGI ≥ 2，高炎症 [HI] 与 mGI 结论相比，高炎症 [HI] 与 mGI 结论相比，高炎症 [HI] 与 mGI 结论相比，高炎症 [HI] 与 mGI 结论相比），结果表明，母体牙龈下微生物的组成与 PTB 有明显的相关性：在高低牙龈炎症水平组中，PTB 和 FTB 母亲的龈下微生物组的组成存在差异。在牙龈炎症水平较高的情况下，就牙周炎而言，PTB 母亲牙菌斑微生物组中的菌群失调比 FTB 母亲少。

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引用次数: 0

Differential expression of FSTL1 and its correlation with the pathological process of periodontitis FSTL1 的差异表达及其与牙周炎病理过程的相关性。

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research

Pub Date : 2024-05-28 DOI: 10.1111/jre.13275

Wenxin Jiang, Weijun Yu, Shucheng Hu, Yuanjie Shi, Lu Lin, Ruhan Yang, Jiaqi Tang, Yuting Gu, Yuhua Gong, Min Jin, Eryi Lu

Aims

This study aimed to elucidate the alterations in Follistatin-like protein 1 (FSTL1) and its association with the pathological process of periodontitis.

Methods

This study included 48 patients with periodontitis and 42 healthy controls. The expression level of FSTL1 in the gingiva was determined by RT-qPCR, validated using the dataset GSE16134, and subsequently examined by western blotting. Bioinformatics analysis revealed a single-cell distribution of FSTL1, characteristic of angiogenesis and immune cell infiltration. The expression and distribution of FSTL1, vascular endothelial marker protein CD31 and myeloperoxidase (MPO), the indicator of neutrophil activity, were determined by immunohistochemistry (IHC). A series of correlation analyses was performed to determine the associations between FSTL1 and clinical parameters, including probing depth (PD) and clinical attachment loss (CAL), and their potential role in angiogenesis (CD31) and neutrophil infiltration (MPO).

Results

FSTL1 was significantly upregulated in the gingiva of patients with periodontitis compared to their healthy counterparts. In addition, FSTL1 was positively correlated with the clinical parameters PD (r = .5971, p = .0005) and CAL (r = .6078, p = .0004). Bioinformatic analysis and IHC indicated that high FSTL1 expression was significantly correlated with angiogenesis and neutrophil infiltration in periodontitis. Moreover, receiver operating characteristic (ROC) analysis demonstrated that FSTL1 could serve as an independent indicator for evaluating the severity of periodontitis (area under the curve [AUC] = 0.9011, p < .0001).

Conclusion

This study demonstrated FSTL1 upregulation in periodontitis and its potential contribution to the disease via angiogenesis and neutrophil infiltration.

目的：本研究旨在阐明类软骨素蛋白1（Follistatin-like protein 1，FSTL1）的变化及其与牙周炎病理过程的关系：研究对象包括 48 名牙周炎患者和 42 名健康对照者。通过 RT-qPCR 测定 FSTL1 在牙龈中的表达水平，并使用数据集 GSE16134 进行验证，随后用 Western 印迹法进行检测。生物信息学分析显示，FSTL1呈单细胞分布，这是血管生成和免疫细胞浸润的特征。免疫组织化学（IHC）测定了 FSTL1、血管内皮标志蛋白 CD31 和中性粒细胞活性指标髓过氧化物酶（MPO）的表达和分布。进行了一系列相关分析，以确定 FSTL1 与临床参数（包括探查深度（PD）和临床附着丧失（CAL））之间的关联，以及它们在血管生成（CD31）和中性粒细胞浸润（MPO）中的潜在作用：结果：与健康患者相比，牙周炎患者牙龈中的FSTL1明显上调。此外，FSTL1 与临床参数 PD（r = .5971，p = .0005）和 CAL（r = .6078，p = .0004）呈正相关。生物信息分析和 IHC 显示，FSTL1 的高表达与牙周炎的血管生成和中性粒细胞浸润显著相关。此外，接收器操作特征（ROC）分析表明，FSTL1 可作为评估牙周炎严重程度的独立指标（曲线下面积 [AUC] = 0.9011，p 结论：FSTL1 的高表达与牙周炎的血管生成和中性粒细胞浸润密切相关：本研究证实了 FSTL1 在牙周炎中的上调及其通过血管生成和中性粒细胞浸润对疾病的潜在贡献。

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引用次数: 0