Journal of Pharmacy Research最新文献

{"title":"Study of phyto-chemical constituents of Ricinus communis Linn. under the influence of industrial effluent","authors":"Kavita Tyagi ,&nbsp;Sandhya Sharma ,&nbsp;Rajat Rashmi ,&nbsp;Sanjiv Kumar","doi":"10.1016/j.jopr.2013.08.005","DOIUrl":"10.1016/j.jopr.2013.08.005","url":null,"abstract":"<div><h3>Aims of the study</h3><p>To carried out the phyto-chemical constituents of <em>Ricinus communis</em> Linn. growing in a highly polluted and non-polluted sites.</p></div><div><h3>Method</h3><p>The industrial effluent was analysed by APHA method. The phyto-chemical constituents were analysed by Cromwell, 1955 &amp; Trease and Evans, 1983 were followed. TLC was analysed by WHO, 1998. Chlorophyll was estimated according to Arnon, 1949.</p></div><div><h3>Results</h3><p>The physico-chemical parameters of analysed effluent were found higher values as compared to standard values. Colour reaction tests showed the degree of changes in plants of polluted sites. The number of spots were decreased in the plant samples of polluted sites. Chlorophyll a, chlorophyll b and total chlorophyll were decreased in those leaves which were collected from polluted sites.</p></div><div><h3>Conclusion</h3><p>It may be concluded that the plants growing at non-polluted areas are not suitable for quality medicines, since, the study reveals quantitative alternations in the chemical constituents of plants growing in industrial areas and other parameters also found declining values in plants collected from polluted area.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 8","pages":"Pages 870-873"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.08.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85907049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of hepatoprotective activity of Swertia chirayita and Andrographis paniculata plant of North–East India against CCl4 induced hepatotoxic rats","authors":"Vinod Kumar Verma,&nbsp;Khomendra K. Sarwa,&nbsp;Atul Kumar,&nbsp;Md. Kamaruz Zaman","doi":"10.1016/j.jopr.2013.07.008","DOIUrl":"10.1016/j.jopr.2013.07.008","url":null,"abstract":"<div><h3>Background</h3><p>The aim of present study was to demonstrate and compare the hepatoprotective activity of ethanol extract of two well-known plants <em>Swertia chirayita</em> Buch-Ham and <em>Andrographis paniculata</em> (Burm.f.) Nees, in Swiss albino rats.</p></div><div><h3>Method</h3><p>The hepatotoxicity induced by single dose of CCl₄ dissolved in olive oil (1 ml/kg b.w.; p.o.) while vehicle control given food and water only. Vehicle as well as hepatotoxic rats were divided into groups (<em>n</em> = 6). Standard group treated with Silymarin (50 mg/kg b.w.; p.o.) daily for 16 days; and treated group received ethanol extract of plant <em>A. paniculata</em> and <em>S. chirayita</em> at the dose of 200 mg/kg b.w. p.o. daily for 16 days respectively.</p></div><div><h3>Results</h3><p>Ethanol extract of plant <em>S. chirayita</em> and <em>A. paniculata</em>, at a dose of 200 mg/kg body weight exhibited protective lowering effects of the serum enzyme levels SGPT, SGOT, GGTP and SALP to a significant extent. The pronounced activity observed in ethanol extract of <em>A. Paniculata</em> with dose of 200 mg/kg (b.w.) however decreases the elevated level of bilirubin, and lipid peroxidase (LPO). The decreased level of TP, GSH, SOD and CAT levels in CCl₄ induced hepatotoxic animal were significantly increase on treatment with ethanol extract of <em>A. Paniculata</em> and <em>S. chirayita</em> plant. The histopathological studies of liver were also supported hepatoprotective activity of <em>A. paniculata</em>.</p></div><div><h3>Conclusion</h3><p>Since results of biochemical studies conclude that the ethanol extract of <em>A. Paniculata</em> showed significant better hepatoprotective as compare to <em>S. chirayita</em>.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 7","pages":"Pages 647-653"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.07.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75307706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hibiscus tiliaceus: A possible immunomodulatory agent","authors":"Guduru Rajeswari ,&nbsp;Balugari Priyanka ,&nbsp;R.E. Amrutha ,&nbsp;Cuddapah Rajaram ,&nbsp;Rupesh S. Kanhere ,&nbsp;Sadhu Nelson Kumar","doi":"10.1016/j.jopr.2013.05.023","DOIUrl":"10.1016/j.jopr.2013.05.023","url":null,"abstract":"<div><h3>Objective</h3><p>The objective of the study is to evaluate the immunomodulatory effect of methanolic leaf extract of <em>Hibiscus tiliaceus</em> (MLHT) in pyrogallol induced immunosuppressed Wistar rats.</p></div><div><h3>Methods</h3><p>The methanolic extract of leaves of <em>H. tiliaceus</em> was administered orally at the dosage levels of 250 mg/kg/day and 500 mg/kg/day b.w in Wistar rats for 28 days. The assessment of immunomodulatory activity, humoral and cellular immunity was studied by hemagglutination antibody (HA) titer, delayed type hypersensitivity (DTH), Neutrophil adhesion test and carbon clearance test. In order to induce immunosuppression in rats pyrogallol (100 mg/kg/day, p.o.) is used and septilin syrup (1ml/100 g/day, p.o.) used as standard as it is immunostimulating agent. Hematological and biochemical were estimated by standard methods.</p></div><div><h3>Results</h3><p>Oral administration of MLHT showed a significant increase in the production of circulating antibody titer in response to sheep red blood cells (SRBCs). A significant (<em>P</em> &lt; 0.001) increase in both primary and secondary HA titer was observed when compared to control group, whereas in <em>H. tiliaceus</em> showed significant (<em>P</em> &lt; 0.001) increase in HA titer. MLHT significantly (<em>P</em> &lt; 0.001) potentiated the DTH reaction by facilitating the footpad thickness response to SRBCs in sensitized rats. Also MLHT evoked a significant (<em>P</em> &lt; 0.001) increase in percentage neutrophil adhesion to nylon fibers and phagocytic activity. It also enhanced the production of RBC, WBC and hemoglobin. It does not much affect the biochemical parameters.</p></div><div><h3>Conclusion</h3><p>An oral administration of the MLHT showed immunomodulatory effect in Wistar rats in a dose dependent manner. From the results obtained and reported phytochemical studies <em>H. tiliaceus</em> has a significant effect on both humoral and cellular immunity in experimental animals, this may be attributed to the polyphenols and flavonoid content of the plant extract.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 7","pages":"Pages 742-747"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.05.023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80324747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant potential of the flowers of Caesalpinia pulcherrima, Swartz in an in vitro system subjected to oxidative stress","authors":"S.T. Yamuna ,&nbsp;P.R. Padma","doi":"10.1016/j.jopr.2013.07.026","DOIUrl":"10.1016/j.jopr.2013.07.026","url":null,"abstract":"<div><h3>Background</h3><p>Oxidative stress leads to various pathological conditions including cancer. Antioxidant enzymes such as superoxide dismutase and catalase represent the cell defense mechanism for preventing oxidative damage. Recently many studies have focused on finding natural antioxidants, especially of plant origin for the treatment of oxidative stress associated diseases. The pharmacological and therapeutic properties of plants are attributed to the ability of antioxidants in them to scavenge free radicals.</p></div><div><h3>Objective</h3><p>In the present study, goat liver was selected as an <em>in vitro</em> model to determine the antioxidant effects of the three flowers (orange, pink and yellow) of <em>Caesalpinia pulcherrima</em> both in the presence and the absence of a standard oxidant (H₂O₂). The enzymic antioxidants (catalase, peroxidase, superoxide dismutase, glutathione reductase and glutathione S-transferase) and the non-enzymic antioxidants (vitamins A, C, E and reduced glutathione) were analysed.</p></div><div><h3>Results</h3><p>Treatment with hydrogen peroxide reduced the antioxidant levels in goat liver slices which were improved on co-treatment with the flower extracts, which proved the antioxidant efficacy of the flowers.</p></div><div><h3>Conclusion</h3><p>Our findings showed that the methanolic extract of the flowers of <em>C. pulcherrima</em> exhibits significant antioxidant activity against H₂O₂-induced oxidative stress in goat liver model.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 7","pages":"Pages 661-665"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.07.026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88154791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic effects of anti-Alzheimer's drug, Galantamine hydrobromide on cholinergic system of mice brain","authors":"Yellamma Kuna ,&nbsp;Nirmala Kumari Borra","doi":"10.1016/j.jopr.2013.06.010","DOIUrl":"10.1016/j.jopr.2013.06.010","url":null,"abstract":"<div><h3>Objectives</h3><p>The present study emphasizes the prolonged effects of an anti-Alzheimer's drug, Galantamine hydrobromide (GHB) on morphometric, behavioural and cholinergic system in mice in the absence of the disease, AD.</p></div><div><h3>Methods</h3><p>One month old male albino mice, <strong><em>Mus musculus</em></strong> (20 ± 2 g) were selected as experimental model and GHB as the test drug. The ED₅₀ dose (5 mg/kg body weight) was given to experimental mice once in a day up to 180 days continuously.</p></div><div><h3>Results</h3><p>Observations on the morphometric aspects such as weight, size and also changes in the behaviour pattern of both control and experimental mice were recorded with help of the Morris water maze technique. Various constituents of the cholinergic system viz. acetylcholine content and acetylcholinesterase level were estimated in different regions of brain such as Olfactory Lobe, Hippocampus, Cerebral Cortex, Cerebellum, Pons-medulla and Spinal cord on selected days during the entire treatment schedule lasting for 180 days through standard biochemical assay techniques. From the results, it was evident that GHB exerted severe perturbations in the cholinergic system in all regions of brain on chronic exposure, thus eventually leading to behavioural changes.</p></div><div><h3>Conclusions</h3><p>From this, it was concluded that GHB, even though exerted positive effects on all the above mentioned parameters which were of course short-lived and during later stages, GHB exerted ill effects. In view of this, particularly, children are cautioned not to consume indiscriminately any kind of memory enhancing drugs or any formulated health drinks containing these chemicals either directly or indirectly for improvement of their cognitive skills.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 7","pages":"Pages 714-719"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78408342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic content of Ruprechtia salicifolia leaf and its immunomodulatory, anti-inflammatory, anticancer and antibacterial activity","authors":"Eman G. Haggag,&nbsp;Mohamed I.S. Abdelhady,&nbsp;Amel M. Kamal","doi":"10.1016/j.jopr.2013.07.015","DOIUrl":"10.1016/j.jopr.2013.07.015","url":null,"abstract":"<div><h3>Objectives</h3><p>This work aimed to isolate phenolics from leaves of <em>Ruprechtia salicifolia</em> and evaluate its immunomodulatory, anti-inflammatory, anticancer and antibacterial activity.</p></div><div><h3>Methods</h3><p>80% MeOH leaf extract was subjected to chromatographic separation, structures of the isolated compounds were established by different chromatographic and spectral techniques UV, MS, ¹H and ¹³C NMR. Immunomodulatory was evaluated through RAW 264.7 macrophages proliferation by MTT assay. Anti-inflammatory was evaluated by inhibition of NO and TNF-α factor generation in LPS-stimulated cells through bicinchoninici acid assay and ELISA assay, respectively. Cytotoxicity estimated through Hep-G2, MCF-7 and HCT-116 cell lines measured by MTT assay. Antibacterial activity tested by agar diffusion method.</p></div><div><h3>Results</h3><p>Eleven known phenolic compounds were isolated for the first time from this species including five flavonoid glycosides <em>viz</em>; Rutin <strong>3</strong>, quercetin 3-<em>O</em>-arabinoglucoside <strong>4</strong>, apigenin 7-<em>O</em>-β-<span>d</span>-glucoside <strong>5</strong>, quercetin 3-<em>O</em>-<em>α-</em><span>l</span>-arabinofuranoside <strong>6</strong> and isoquercetin <strong>7</strong> along with four aglycones <em>viz</em>; kaemferide <strong>8</strong>, apigenin <strong>9</strong>, quercetin <strong>10</strong> and naringenin <strong>11</strong> and two phenolic acids; caffeic <strong>1</strong> and gallic <strong>2</strong>. Compounds <strong>5</strong> showed the most activity increasing macrophage proliferation implying immunomodulatory activity. 80% MeOH extract, <strong>4</strong>, <strong>5</strong> and <strong>11</strong> inhibited nitrite oxide by 68.19%, 52.95%, 20.33% and 15.22%, respectively and TNF-α generation by 70.82%, 29.88%, 13.13% and 6.14%, respectively in LPS-stimulated cells implying anti-inflammatory activity. 80% MeOH leaf extract and the tested compounds <strong>4</strong> and <strong>5</strong> were safe possessing no cytotoxic activity against hepatocellular carcinoma (Hep-G2), breast adenocarcinoma (MCF-7) and colon carcinoma (HCT-116), while Compound <strong>11</strong> had cytotoxicity against only HCT-116 cells (IC₅₀ = 27.67 μg/ml). Also 80% MeOH leaf extract showed antibacterial activity against both G +ve and G −ve bacteria, moreover it inhibits growth of <em>Klebsiella pneumonia</em> strain, which is resistant to Ciprofloxacin broad-spectrum antibiotic.</p></div><div><h3>Conclusions</h3><p><em>R. salicifolia</em> contain phenolics of immunomodulatory anti-inflammatory, cytotoxicity and antibacterial activity, giving <em>R. salicifolia</em> grate potential as a medicinal natural drug.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 7","pages":"Pages 696-703"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.07.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81634953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico drug docking and screening for the drug discovery of new tyrosinase inhibitors","authors":"Sapna S. Ingle,&nbsp;Chandrahas N. Khobragade","doi":"10.1016/j.jopr.2013.06.021","DOIUrl":"10.1016/j.jopr.2013.06.021","url":null,"abstract":"<div><h3>Aim</h3><p>To investigate potent tyrosinase inhibitor by drug docking analysis.</p></div><div><h3>Methods</h3><p>The study involved the protein structure of tyrosinase of <em>B. megaterium</em> to investigate the drugs designed by Chem office and drug docking was performed by AutoDock to investigate QSAR activity of the drug.</p></div><div><h3>Results</h3><p>Tyrosinase is an important enzyme linked with disorders like Parkinson's, melanogenesis, and hyper pigmentation, and studies on selection tyrosinase inhibitor and its implication in drug therapy is an urgent need. We have investigated five drugs which showcased tyrosinase inhibitor activity when tested by QSAR analysis in AutoDock. Docking study was done with the tyrosinase of <em>B. megaterium</em>, and results highlighted potent binding affinity of the drugs with binding energy in the range of −06.00 kcal/mol. In view, designed drugs show potential as tyrosinase inhibitor and may be used further for study.</p></div><div><h3>Conclusion</h3><p>All five drugs docked successfully with binding energy in the range of −06.00 kcal/mol suggested significant results.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 7","pages":"Pages 704-708"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81738482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel validated stability-indicating UPLC method for the determination of Metoclopramide and its degradation impurities in API and pharmaceutical dosage form","authors":"Prathyusha Sowjanya ,&nbsp;Palani Shanmugasundaram ,&nbsp;Petla Naidu ,&nbsp;Sanjeev Kumar Singamsetty","doi":"10.1016/j.jopr.2013.07.004","DOIUrl":"10.1016/j.jopr.2013.07.004","url":null,"abstract":"<div><h3>Aim</h3><p>To develop a stability-indicating reversed phase ultra performance liquid chromatographic (RP-UPLC) method for the determination of related substances in Metoclopramide bulk drugs and pharmaceutical dosage form.</p></div><div><h3>Method</h3><p>The chromatographic separation was achieved using a Waters X-terra RP18 (150 × 4.6 mm), 3.5 μm particle size column using the gradient program with mobile phase consisting of solvent A: 30 mM monobasic sodium phosphate and 2.3 mM of pentane-1-sulphonic acid sodium salt (pH 3.0 buffer) and solvent-B (Acetonitrile). A flow rate of 1.2 mL/min and UV detector at 273 nm was used. The runtime was 18 min within which Metoclopramide and its four impurities, ACETYLMETO, ACMA, CLEE and ACME were well separated.</p></div><div><h3>Results and discussion</h3><p>The drug was subjected to stress conditions such as oxidative, acid &amp; base hydrolysis, thermal and photolytic degradation. Metoclopramide was found to degrade significantly in photolytic, oxidative &amp; thermal stress conditions and stable in acid, base, hydrolytic &amp; humidity stress conditions. The major degradation impurities in oxidation and photolytic degradation were identified by LCMS. The degradation products were well resolved from the main peak and its impurities, thus proved the stability-indicating power of the method.</p></div><div><h3>Conclusion</h3><p>The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness. The calibration curves obtained for the four impurities were linear over the range 0.062–3.040 μg/mL.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 7","pages":"Pages 765-773"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.07.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81469690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of foodborne pathogen Listeria monocytogenes strain Pyde1 and Pyde2 using 16S rRNA gene sequencing","authors":"Acharya Nagarjun Pyde ,&nbsp;P. Nagaraja Rao ,&nbsp;Aditya Jain ,&nbsp;Divya Soni ,&nbsp;Shailesh Saket ,&nbsp;Sheaza Ahmed ,&nbsp;Sugunakar Vuree ,&nbsp;Anuraj Nayarisseri","doi":"10.1016/j.jopr.2013.07.009","DOIUrl":"10.1016/j.jopr.2013.07.009","url":null,"abstract":"<div><h3>Aim</h3><p><em>Listeria monocytogenes</em> acts as a pathogen for humans and animals, mainly causing, neonatal sepsis, abortions in pregnant females and severe infections such as septicemia and meningoencephalitis in susceptible hosts. Current study was aimed to identify novel strains of <em>L. monocytogenes</em> from retail chicken, beef meat and seafood samples.</p></div><div><h3>Methods</h3><p>In order to identify the strain, extraction and amplification of genomic DNA, 16S rRNA sequence analysis was carried out. Phylogenetic trees were constructed using dnapars and dnaml available in Phylip. The secondary structures of 16S rRNA gene sequence were predicted using UNAFOLD, a Linux based software.</p></div><div><h3>Results</h3><p>The results obtained were found to be a novel foodborne pathogens, which was further named <em>L. monocytogenes</em> strain <em>Pyde1</em> and <em>L. monocytogenes</em> strain <em>Pyde2</em>, after characterization the sequence of isolate was deposited in GenBank with accession numbers ‘KC852899’ and ‘KC852900’ respectively. The Gibb's free energy of the secondary structures of <em>L. monocytogenes</em> strain <em>Pyde1</em> and <em>Pyde2</em> were −275.60 and −282.20 kcal/mol seems to be more stable in the present investigation.</p></div><div><h3>Conclusion</h3><p>The described results of phylogenetic distinctiveness and phenotypic disparities indicate that strain 2b represents a novel strain of foodborne pathogens within <em>L. monocytogenes</em> species, for which the name <em>L. monocytogenes</em> strain <em>Pyde1</em> and <em>L. monocytogenes</em> strain <em>Pyde2</em> is proposed.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 7","pages":"Pages 736-741"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.07.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88070963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reverse vaccinology: An approach to search vaccine leads of Shigella sonnei","authors":"Yatharth Anand ,&nbsp;Sunil Pande ,&nbsp;Dilip Gore","doi":"10.1016/j.jopr.2013.07.030","DOIUrl":"10.1016/j.jopr.2013.07.030","url":null,"abstract":"<div><h3>Aim</h3><p>To develop vaccine leads by reverse vaccinology approach for <em>Shigella sonnei</em>.</p></div><div><h3>Materials and methods</h3><p>Study screened the coding proteins of <em>S. sonnei</em> as vaccine targets collected from NCBI website and filtered as cell surface proteins using programs such as SignalP 4.1, TMHMM, LipoP 1.0, PsortB and BLASTP.</p></div><div><h3>Observation and results</h3><p>In recent years due to rapid development in genome sequencing technology vaccine development program has achieved defined specificity and success. In our study, in total 63 cell surface proteins as vaccine leads were searched which are highly conserved among genus Shigella.</p></div><div><h3>Conclusion</h3><p>Study successfully used reverse vaccinology in search of vaccine leads in <em>S. sonnei</em>.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 7","pages":"Pages 576-581"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.07.030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82460488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}