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Assessment of Antimicrobial and Antioxidant Activities of Four Ethnomedicinal Plants Used by Magars in Nawalpur District, Nepal 尼泊尔纳瓦尔布尔地区马格尔人使用的四种民族药用植物的抗菌和抗氧化活性评价
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i2.57030
Chandra Mohini Nemkul, G. B. Bajracharya, Ila Shrestha
Ethnobotanical survey in the Magar villages in Nawalpur district, Gandaki province, Nepal revealed that root of Phanera vahlii (Wight & Arn.) Benth, bark of Rhododendron arboreum Sm. and flower of Woodfordia fruticosa (L.) Kurz, have been in use for diarrhea and dysentery. Root of Thalictrum foliolosum DC. has been used for the treatment of rheumatic pain. The study aimed to evaluate antimicrobial and antioxidant effects as well as total phenol content in Phanera vahlii, Rhododendron arboreum, Woodfordia fruticosa and Thalictrum foliolosum. Total phenol content (TPC) was estimated using Folin-Ciocalteu method. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical and hydrogen peroxide scavenging assays were used to evaluate the antioxidant capacity. Antibacterial effect was established by the Agar well diffusion assay. The minimum TPC value of 65.78±3.44 mg GAE/g dry extract was estimated in 70% methanolic extract of Thalictrum foliolosum and maximum TPC value of 258.40±6.26 mg GAE/g dry extract was estimated in 70% methanolic extract of Woodfordia fruticosa. IC50 value range 21.59±0.26 µg/ml in Rhododendron arboreum to 1124.79±3.69 µg/ml in Thalictrum foliolosum was calculated in DPPH free radical scavenging assay. Scavenging of DPPH free radical range from 12.40% to 94% at 100 µg/mL concentration of 70% methanolic extracts were estimated and scavenging of hydrogen peroxide range from 36% to 73%. The maximum zone of inhibition (ZOI) against Escherichia coli at loading dose of 5 mg of the extract observed was 18±0.73 mm by 70% methanolic extract of Woodfordia fruticosa and its minimum inhibition concentration (MIC) was <1.56 µg /ml.The extracts efficiently inhibited the growth of Escherichia coli verifying the rural knowledge. At the same time, the extracts displayed efficient antioxidant activity.
在尼泊尔甘达基省纳瓦尔布尔地区的Magar村进行的民族植物学调查显示,Phanera vahlii的根(wright & Arn.)下层,杜鹃花树皮。和花的木福特果(L.)库尔茨,已被用于腹泻和痢疾。圆锥花序的根。已被用于治疗风湿性疼痛。本研究旨在评价白翅、杜鹃花、木本草和垂叶草的抗菌和抗氧化作用及总酚含量。用Folin-Ciocalteu法测定总酚含量(TPC)。采用2,2 -二苯基-1-吡啶酰肼(DPPH)自由基和过氧化氢清除实验来评价其抗氧化能力。通过琼脂孔扩散实验确定其抑菌效果。70%甲醇提取物的TPC值最小为65.78±3.44 mg GAE/g, 70%甲醇提取物的TPC值最大为258.40±6.26 mg GAE/g。DPPH自由基清除实验中,杜鹃花的IC50值为21.59±0.26µg/ml ~小叶樱的IC50值为1124.79±3.69µg/ml。在100µg/mL浓度下,70%甲醇提取物对DPPH自由基的清除率为12.40% ~ 94%,过氧化氢的清除率为36% ~ 73%。70%甲醇提取物对大肠杆菌的最大抑制区(ZOI)为18±0.73 mm,最小抑制浓度(MIC) <1.56µg /ml。提取物有效抑制大肠杆菌的生长,验证了农村知识。同时,提取物具有较强的抗氧化活性。
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引用次数: 0
Traditionally Important Herbaceous Medicinal Plants of Majathal Wildlife Sanctuary, District Solan, Himachal Pradesh, North Western Himalaya, India 传统重要草本药用植物,印度喜马偕尔邦索兰区,喜马拉雅西北部
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i2.57035
K. Kumari, R. Verma
The Himalayas is one of the mega diversity hotspots of the world, which contribute a great to the biodiversity wealth of the world. Among the biogeographic provinces of India, the Indian Himalayan Region (IHR) is well known for its diversity of ethnomedicinal plants. Majathal Wildlife Sanctuary is situated in District Solan, Himachal Pradesh, North Western Himalaya, India. In the present study, social surveys were conducted through the application of semi-structured questionnaire. All 39 villages inside and near the boundary (up to 2 km) were selected for the survey in the sanctuary. All the houses were surveyed and people of different ages and sexes were asked and their responses were recorded. A reconnaissance survey was conducted for the floristic diversity in the area and plant samples were collected for identification and herbarium preparation. In the sanctuary, there were 51 species of herbaceous medicinal plants identified which were used by local people. The results showed that the knowledge regarding medicinal plants was very less in the new generation as compared to the old age and middle age people. This study will help to know more medicinal plants from the villages and procure knowledge for future studies. This documented information will be preserved and utilized for mankind and make local communities economically stable and healthy.
喜马拉雅山脉是世界上最大的生物多样性热点地区之一,为世界生物多样性财富做出了巨大贡献。在印度的生物地理省份中,印度喜马拉雅地区(IHR)以其民族药用植物的多样性而闻名。Majathal野生动物保护区位于印度喜马拉雅西北部喜马偕尔邦的索兰区。本研究采用半结构式问卷进行社会调查。在保护区内和边界附近(2公里以内)的39个村庄都被选中进行调查。调查了所有的房子,询问了不同年龄和性别的人,并记录了他们的回答。对该地区的植物区系进行了调查,并采集了植物标本进行鉴定和标本室制作。在保护区内,已鉴定出51种当地居民使用的草本药用植物。结果表明,与中老年人群相比,新生代对药用植物的认识较少。这项研究将有助于了解更多来自村庄的药用植物,为未来的研究积累知识。这些记录在案的信息将为人类保存和利用,并使当地社区经济稳定和健康。
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引用次数: 0
Anatomical Study of Shorea robusta Gaertn. 竹笋的解剖学研究。
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i01.56599
P. Chalise, Y. R. Paneru, L. Joshi
Shorea robusta, commonly known as Sal, is one of the multipurpose timber trees in Nepal. This study aims to carry out detailed anatomical investigation of the wood and leaf anatomical traits of Sal. The wood and leaf samples of Sal were collected from Rupandehi district, Nepal. Wood of Sal was found to be diffuse-porous and the vessels were mostly solitary or paired and sometimes in a short radial multiple. Non-septate, vasicentric fibres forming solid tracts connected the vessels and rays; vasicentric parenchyma with lozenge aliform to aliform confluent arrangement; moderately broad, multiseriate rays and the presence of prismatic crystals in rays were the characteristic feature of the wood. Leaves were hypostomatic and the stomata were cyclocytic with four or more subsidiary cells surrounding the guard cells. Stellate, glandular trichomes were present in upper epidermis, while unicellular, simple, glandular, tufted trichomes were present in the lower epidermis. The increase in global trade has resulted in over-exploitation of forest resources, and hence in the present context, the identification and traceability of wood is highly crucial. The outcomes of this study are supposed to help in the identification of wood of Sal through their anatomical study.
Shorea robusta,俗称Sal,是尼泊尔的一种多用途木材树。本研究旨在对萨尔木的木材和叶片解剖特征进行详细的解剖研究。萨尔树的木材和叶子样本采集于尼泊尔鲁潘德希地区。木材呈弥漫性多孔,血管多为单生或成对,有时呈短的径向多重。无间隔的血管中心纤维形成连接血管和射线的实束;vasicentry薄壁组织呈菱形翼形到翼形合流排列;适度宽,多系列的射线和射线中棱柱状晶体的存在是木材的特征。叶片低气孔,气孔呈环状,保卫细胞周围有四个或更多的附属细胞。星状、腺状毛存在于上表皮,单细胞、单细胞、腺状、簇状毛存在于下表皮。全球贸易的增加造成了对森林资源的过度开发,因此在目前情况下,木材的鉴定和可追溯性是非常关键的。本研究的结果应该有助于通过对其解剖研究来识别萨尔木。
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引用次数: 0
Formulation of Herbal Tea from Nepalese Medicinal Plants: Phenolic Assay, Proximate Composition and In-vivo Toxicity Profiling of Medicinal Plants with Nutritive Benefits 尼泊尔药用植物草药茶的配方:具有营养价值的药用植物的酚含量测定、近似成分和体内毒性分析
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i01.56603
A. Mandal, Anisha Pandey, Prasamsha Pant, Seema Sapkota, P. Yadav, D. P. Bhandari
Herbal tea, also known as tisane, is a beverage made from the infusion or decoction of plant material in hot water. True tea comes from the Camellia sinensis plant, while tisane (herbal tea) comes from a water-based infusion of herbs, spices, flowers, leaves etc. This study aimed to formulate and determine the nutraceutical value (proximate analysis), phytochemical value (total phenolic content) and in-vivo toxicity of the different medicinal plants used to prepare three different types of herbal tea formulations. Natural Product Research Laboratory (NPRL)-1 [Asparagus officinalis L., Phyllanthus emblica L., Mentha piperita L., Elettaria cardamomum (L.) Maton and Camellia sinensis (L.) Kuntze], NPRL-2 [Ocimum  tenuiflorum L., Bergenia ciliate (Haw.) Sternb., Elettaria cardamom (L.) maton and Camellia sinensis (L.) Kuntze] and NPRL-3 [Salvia rosmarinus Spenn., Cymbopogon citratus (DC.) Stapf, Senegalia catechu (L.f.) P.J.H.Hurter & Mabb.  Elettaria cardamomum (L.) Maton and Withania somnifera (L.) Dunal] herbal tea formulations were prepared from these selected medicinal plants. They were respectively tested for their properties. All the plants included were highly nutritional and none were found toxic. The results suggested that herbal tea made up of these potent plants’ parts can be a good choice for health-promoting benefits. These formulations could further be studied for their other beneficial activities.
草药茶,也被称为tisane,是一种由植物材料在热水中浸泡或煎煮而成的饮料。真正的茶来自茶树,而草药茶(草药茶)来自草药、香料、花、叶子等的水基浸渍。本研究旨在配制并测定三种不同类型凉茶制剂所用不同药用植物的营养价值(近似分析)、植物化学价值(总酚含量)和体内毒性。天然产物研究实验室(NPRL)-1[芦笋,甘油三酯,薄荷,豆蔻]山茶花(L.)Kuntze, NPRL-2[紫菀,紫菀(Haw.)]。Sternb。、小豆蔻(L.)、山茶(L.)和NPRL-3[鼠尾草迷迭香]。, Cymbopogon citratus (DC.)Stapf,塞内加尔儿茶(l.f.)p.j.h.赫特&马布公司。豆蔻Elettaria cardamomum马顿草(L.)草药茶配方是从这些精选的药用植物中制备的。分别测试了它们的性能。所有的植物都是高营养的,没有发现有毒。结果表明,由这些强效植物成分组成的凉茶是促进健康的好选择。可以进一步研究这些配方的其他有益活性。
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引用次数: 0
Cladistic Analysis of Morpho-anatomical Data of Subtribe Coelogyninae of Nepal Himalaya 尼泊尔-喜马拉雅地区腔母亚族形态解剖资料的分支分析
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i2.56981
D. Bajracharya, Gita Dongol, S. Rajbhandary
A cladistic analysis of subtribe Coelogyninae based on 10 morphological and 19 anatomical characters of 20 species of Coleogynae, Pholidota, Panisea and Pleione from Nepal was done. Most parsimonious tree were traced using computer assisted software NONA, Winclada (123 trees with CI = 35 and RI = 57). The analysis confirms the monophyletic origin of the subtribe Coelogyninae and species assorted into two different clades. Clade I is separated by round to oval mesophyll cell shape, such as in the species of Otochilus, Panisea and Pleione. Whereas the clade II is separated by stomatal index value 10-15. The results of cladistic analysis suggest that the subtribe Coelogyninae is monophyletic.
摘要根据尼泊尔共20个科属(Coleogynae, Pholidota, Panisea, Pleione)的10个形态学特征和19个解剖学特征,对尼泊尔共20个科属(Coleogynae)亚族进行了分支分析。大多数节俭树使用计算机辅助软件NONA, Winclada进行追踪(123棵树,CI = 35, RI = 57)。这一分析证实了尾女亚族的单系起源,以及分属两个不同支系的物种。分支I以圆形到椭圆形的叶肉细胞形状分开,例如在Otochilus, Panisea和Pleione种中。II枝的气孔指数值为10 ~ 15。支系分析结果表明,该亚部落是单系的。
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引用次数: 0
Growth Response of Pinus wallichiana to Changing Climate in Temperate Regions of Central Nepal 尼泊尔中部温带地区瓦利恰纳松生长对气候变化的响应
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i01.56595
Tulasi Shiwakoti, N. Thapa, Saroj Basnet, A. Tiwari
Climate influences both the structure and function of the forest ecosystem. A dendrochronological study was carried out in temperate forest at Patney Bhanjyang Community Forest of Kavrepalanchok district in Central Nepal to verify and record the impact of climate on radial growth by using the tree cores of Pinus wallichiana. A total of 60 tree cores were collected by using increment borer and annual ring widths were analyzed by using the standard dendrochronological technique. We produced a 74-year-old tree ring width chronology of P. wallichiana from the region and examined the critical climatic factor for radial growth and the temporal trend of Basal Area Increment (BAI). Our results showed that the tree radial growth was not influenced by maximum temperature. However, it was positively correlated with the minimum temperature of the previous year September (0.30) indicating that cool previous September is favorable for radial growth. Similarly, the radial growth of Pine showed a positive correlation with the total rainfall of January (0.33) and March (0.33), showing that enough moisture in the very early growing season contributes positively to radial growth. We found a decline in BAI after 2000 AD until 2005, although BAI was relatively stable despite the decreasing trend of rainfall. We did not find the impact of climatic factor for the decline in BAI. However long-term study of different climatic, ecological and anthropogenic influences are necessary to know more about the growth–climate relationship of P. wallichiana in temperate forests of Nepal.
气候对森林生态系统的结构和功能都有影响。在尼泊尔中部kavrepalanchuk地区的Patney Bhanjyang社区森林进行了一项树木年代学研究,利用wallichiana松的树芯验证和记录了气候对径向生长的影响。采用增量钻孔法采集了60棵树芯,采用标准树木年轮技术对年轮宽度进行了分析。利用74年的年轮宽度年代学,分析了影响柽柳径向生长的关键气候因子和基础面积增量(BAI)的时间变化趋势。结果表明,最高温度对树的径向生长没有影响。但与往年9月最低气温呈显著正相关(0.30),表明往年9月气温较低有利于径向生长。松树的径向生长与1月(0.33)和3月(0.33)的总降雨量呈正相关,说明生长期早期充足的水分对径向生长有积极的促进作用。从2000年到2005年,BAI呈下降趋势,尽管降水呈下降趋势,但BAI相对稳定。我们没有发现气候因子对BAI下降的影响。然而,要进一步了解尼泊尔温带森林中白杨的生长与气候关系,还需要对不同的气候、生态和人为影响进行长期研究。
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引用次数: 0
Ethnobotany of Medicinal Plants Used by Kathariya (Tharu) Community in Kailali District, Nepal 尼泊尔Kailali地区Kathariya (Tharu)社区药用植物的民族植物学研究
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i2.57038
Chetana Khanal, Sudhir Neupane, Madhu Shudan Thapa Magar
This study aims to document the traditional knowledge on medicinal use of plants by Kathariya (Tharu) community in Kailali district. Kathariya is one of the sub-group of the Tharu community whose culture and language is slightly different from other sub-groups. Traditional healers Guruwa of Kathariya community from five study sites viz. Sisaiya, Tappa, Udasipur, Pawera and Joshipur of Kailali were identified as key informants. Exploratory research design was adopted and interview method was used to collect the primary information through Semi-structured open-ended questionnaire. Altogether 70 species of plants used for medicinal purposes were documented. Sixteen species were found useful in cuts, wounds and swelling which is one of ten health issues categorized in the present study. Thirteen and Twelve species were recorded to be used in abdominal disorders and skin diseases respectively. The community usually mixes powder in measurement of two and half grains of each Barley and Black Pepper during preparations of medicine for oral route of consumption. Based on habit, highest number of plant species belongs to herbs (30 species). Among five study sites, highest number of species was recorded in Udasipur with 35 species. Regarding the parts of plant used, roots and leaves were found to be used often  equally, which were represented by 24 species each. Achyranthes aspera, Euphorbia hirta, Azadirachta indica and Clerodendrom infortunatum were the most frequently used species. This study generated preliminary but crucial information which may open the avenue for utilization of biological resources. Further research leading to bio-prospecting is needed to utilize these resources.
本研究旨在记录Kailali地区Kathariya (Tharu)社区关于植物药用的传统知识。Kathariya是Tharu社区的一个分支,其文化和语言与其他分支略有不同。来自五个研究地点(Sisaiya, Tappa, Udasipur, Pawera和Kailali的Joshipur)的Kathariya社区的传统治疗师Guruwa被确定为关键信息提供者。采用探索性研究设计,访谈法通过半结构化开放式问卷收集主要信息。共有70种药用植物被记录在案。16种被发现对切割、伤口和肿胀有用,这是本研究中分类的十大健康问题之一。记录了13种和12种分别用于腹部疾病和皮肤疾病。在配制口服药物时,社区通常将每种大麦和黑胡椒的2.5粒粉末混合在一起。从习性上看,植物种类最多的是草本植物(30种)。在5个研究点中,乌达西浦尔的物种数量最多,有35种。在植物的使用部位上,根和叶的使用往往相同,分别有24种。牛膝草、大戟、印楝和芒萝是最常用的植物。这项研究产生了初步但重要的信息,可能为生物资源的利用开辟道路。利用这些资源需要进一步的生物勘探研究。
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引用次数: 0
Insights from Local Community on Changing Availability of Non-Timber Forest Products under Climate Change in Panchadeval Binayak Municipality, Achham District, West Nepal 气候变化背景下当地社区对尼泊尔西部阿查姆地区Panchadeval Binayak市非木材林产品供应变化的见解
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i2.57048
Sabina Shahi, H. Paudel, Shiba Raj Ghimire
Local people in the hilly area depend upon different varieties of non-timber forest products (NTFPs) such as foods, medicines, spices, etc. for income generation, except timber. This paper highlights the perception of local people over current situation including status of availability of NTFPs under changing climate. Data were collected by using questionnaire survey and key informant interview (KII) in 9 wards of Panchadeval Binayak Municipality during April, 2022. Altogether 225 interviews were taken with the oldest family members of each household. Total 89 species of NTFPs were noted. Majority of species were used for medicinal purpose (53.92%), followed by edible (30.33%) whereas 15.73% species were used for fodder, fuelwood and other purposes. Climate change and availability of NTFPs were analyzed according to people’s perception. The study showed that about 96% of respondents agreed to increase in temperature, 84.83% believed in decrease of rainfall, whereas13.33% agreed the rainfall to be unpredictable. Due to those climatic changes, the availability of NTFPs is predicted to decrease and the dependency of people on NTFPs is in critical condition in this municipality. Therefore, it is urged to develop some climate change coping strategies at the local and national level which will assure the NTFPs availability for the local people.
山区的当地人依靠不同种类的非木材林产品(NTFPs),如食品、药品、香料等来创收,但木材除外。本文强调了气候变化下当地居民对现状的看法,包括ntfp的可用性状况。于2022年4月对比纳亚克市9个病区采用问卷调查和关键知情者访谈法收集数据。与每个家庭最年长的家庭成员共进行了225次访谈。共发现nntfp 89种。药用品种最多(53.92%),食用品种次之(30.33%),饲料、薪材和其他用途占15.73%。根据人们的认知,分析了气候变化和非森林保护区的可得性。研究表明,约96%的受访者认为气温会升高,84.83%的受访者认为降雨量会减少,而13.33%的受访者认为降雨量不可预测。由于这些气候变化,预计可获得的非森林资源将减少,该市人民对非森林资源的依赖处于危急状态。因此,敦促在地方和国家一级制定一些应对气候变化的战略,以确保当地人民获得国家森林保护区。
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引用次数: 0
Toxicity Test of Some Selected Wild Mushrooms of Nepal 尼泊尔几种野生蘑菇的毒性试验
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i01.56602
Madhu Ghimire, H. Aryal, P. B. Lakhey, Rajeshwor Ranjitkar, Yagya Raj Bhatta
People have been using wild mushrooms in their diet and also as sources of income, but most of them do not have the proper knowledge to distinguish edible mushrooms from poisonous ones. The main objective of this research is to determine and document toxicity of some selected wild mushrooms of Nepal. Samples were collected based on their use practices by the local communities, representing different ecological belts. The tests were carried out following the protocol described in ‘Test Guideline no. 425: Acute Oral Toxicity: Up-and-Down Procedure’. Mice (Mus musculus) were administered with aqueous solution of each sample in a single dose by gavage using a stomach tube on the basis of their body weight and were observed for the signs of toxicity. The tested samples of Boletellus emodensis, Caloboletus calopus, Daedalea quercina, Lactifluus volemus, Lyophyllum decastes, Macrolepiota albuminosa, Phellodon niger, Phylloporus bellus, Russula delica, R. emetica, R. senecis, Strobilomyces strobilaceus, Termitomyces eurrhizus, T. le-testui, T. mammiformis, T. robustus, T. striatus f. brunneus, T. straitus f. pileatus, Trametes vernicipes and Trichaptum biforme revealed LD50 value > 2000 mg.kg-1 body weight indicating that they may be harmful if swallowed . However, in case of Hapalopilus rutilans, LD50 value of 1212 mg.kg-1 body weight was observed confirming that it is harmful when swallowed.
人们一直在饮食中使用野生蘑菇,也将其作为收入来源,但大多数人没有适当的知识来区分食用蘑菇和有毒的蘑菇。本研究的主要目的是确定和记录尼泊尔一些选定的野生蘑菇的毒性。代表不同生态带的当地社区根据其使用实践收集样本。试验按照“试验指南no. 5”中描述的方案进行。425:急性口服毒性:上下程序。小鼠(小家鼠)按体重用胃管灌胃给药,每次给药一次,观察毒性体征。检测样品中大黄带孢霉、大黄带孢霉、大黄带孢霉、白垩带孢霉、黑黄柏、bellloporus、Russula delica、吐乳霉、senecus、Strobilomyces strobilces、eurrhizomyces、le-testui、乳状霉、robustus、brunneus纹状霉、pileatus纹状霉、tremetes vernicipes和Trichaptum biformme的LD50值均> 2000 mg。体重1公斤,说明吞食后可能有害。然而,对于芦丁,LD50值为1212mg。经观察,体重为Kg-1,证实吞食有害。
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引用次数: 0
Grass Flora along Altitudinal Gradient of the Phulchoki Hill, Central Nepal 尼泊尔中部Phulchoki山沿海拔梯度的草区系
Pub Date : 2022-12-31 DOI: 10.3126/bdpr.v20i01.56579
Anjana Kharbuja, S. Rajbhandary
Phulchoki hill on the southern part of Kathmandu Valley (1550-2750 m) presents a unique opportunity to study the diversity of grasses along the altitudinal gradient. During the present study, 73 species of grasses belonging to 5 sub families, 16 tribes and 48 genera were recorded, out of which 28 species are new to this area. Among the recorded species 89% of the species were terrestrial, 7% lithophytes and remaining 4% aquatic. The lowest elevation (1550-1950 m) has highest diversity with 49 species whereas the topmost band (2351-2750 m) has least diversity with only 19 species. The total species richness of grass has decreasing trend along the altitudinal gradient of species, with r2 =0.97 and p=0.04, which indicates significant relation.
位于加德满都谷地南部(海拔1550-2750米)的Phulchoki山为研究沿海拔梯度的草类多样性提供了一个独特的机会。本研究共记录到禾本科植物73种,隶属于5亚科16族48属,其中28种为本地区新发现。在记录的物种中,89%为陆生物种,7%为岩生物种,其余4%为水生物种。最低海拔(1550 ~ 1950 m)的多样性最高,有49种,而最高海拔(2351 ~ 2750 m)的多样性最低,只有19种。禾草总物种丰富度沿物种高度梯度呈下降趋势,r2 =0.97, p=0.04,相关性显著。
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引用次数: 0
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Journal of Plant Resources and Environment
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