Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i01.56577
Madhu Shudan Thapa Magar, Seerjana Maharjan, Januka Pathak, D. Kandel, Ganga Rijal
Sphagnum nepalense is a bryophyte endemic to Nepal. The objective of the present study is to analyze DNA barcoding markers useful for delineating the Sphagnum species. Here, a specimen of Sphagnum nepalense collected from the bank of Maipokhari lake, Ilam (2107 m asl) was used. Three chloroplast loci from the sample viz. rbcL, psbA-trnH and trnF-trnL, the latter two being intergenic spacers, were amplified and sequenced. Four accessions of plastome sequences of S. junghuhnianum, S. multifibrosum, S. palustre and S. subsecundum were retrieved from the National Center for Biotechnology Information (NCBI). Evolutionary analysis was performed following the Maximum Likelihood approach using MEGA X. The result showed that the evolutionary tree generated with single locus trnF-trnL and combined sequences of trnF-trnL and psbA-trnH was better compared to that generated with the sequence of other single locus and even the combined sequence of rbcL, psbA-trnH and trnF-trnL. The sequence data generated in this study for Sphagnum nepalense are novel to the scientific community.
{"title":"Some Barcoding DNA Sequence Analysis of Sphagnum nepalense H. Suzuki, a Bryophyte Species Endemic to East Nepal","authors":"Madhu Shudan Thapa Magar, Seerjana Maharjan, Januka Pathak, D. Kandel, Ganga Rijal","doi":"10.3126/bdpr.v20i01.56577","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56577","url":null,"abstract":"Sphagnum nepalense is a bryophyte endemic to Nepal. The objective of the present study is to analyze DNA barcoding markers useful for delineating the Sphagnum species. Here, a specimen of Sphagnum nepalense collected from the bank of Maipokhari lake, Ilam (2107 m asl) was used. Three chloroplast loci from the sample viz. rbcL, psbA-trnH and trnF-trnL, the latter two being intergenic spacers, were amplified and sequenced. Four accessions of plastome sequences of S. junghuhnianum, S. multifibrosum, S. palustre and S. subsecundum were retrieved from the National Center for Biotechnology Information (NCBI). Evolutionary analysis was performed following the Maximum Likelihood approach using MEGA X. The result showed that the evolutionary tree generated with single locus trnF-trnL and combined sequences of trnF-trnL and psbA-trnH was better compared to that generated with the sequence of other single locus and even the combined sequence of rbcL, psbA-trnH and trnF-trnL. The sequence data generated in this study for Sphagnum nepalense are novel to the scientific community.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72707589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i2.56773
S. Dhakal, N. Ghimire, S. Rai, S. Dhakal
Algae can be found in the sea, freshwater and wastewater all around the world. Although the majority is microscopic, some are large. In this study, a total of 44 samples, were collected in two seasons (pre-monsoon and post-monsoon) in the years 2021-2022 from Kingfisher Lake, located in the Bharatpur, Chitwan District. A total of 51 algal species belonging to six classes were recorded. Information on the distribution of algal flora of Kingfisher Lake was collected and the data revealed that the dominant members belong to the classes Bacillariophyceae and Zygnematophyceae, each having 15 species; followed by Chlorophyceae, Cyanophyceae and Euglenophyceae. Similarly, the physico-chemical parameters of water were measured by a digital portable multiparameter (HI98194) in the field. Water temperature was low during post-monsoon season. Electrical conductivity, Total Dissolved Solids (TDS), Oxidation Reduction Potential and Dissolved Oxygen were high in post-monsoon season while the pH of the lake water was alkaline and low pH in post-monsoon season. The number of algal species was found higher in pre-monsoon season than in post-monsoon season. The study showed that the lake is rich in algal species. Presence of the species like Closterium sp. and Oscillatoria sp. indicate that the lake is eutrophicated and polluted. Thus, further investigations were needed.
{"title":"Seasonal Variations of Algae in Relation to the Water Quality at Kingfisher Lake, Central Nepal","authors":"S. Dhakal, N. Ghimire, S. Rai, S. Dhakal","doi":"10.3126/bdpr.v20i2.56773","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.56773","url":null,"abstract":"Algae can be found in the sea, freshwater and wastewater all around the world. Although the majority is microscopic, some are large. In this study, a total of 44 samples, were collected in two seasons (pre-monsoon and post-monsoon) in the years 2021-2022 from Kingfisher Lake, located in the Bharatpur, Chitwan District. A total of 51 algal species belonging to six classes were recorded. Information on the distribution of algal flora of Kingfisher Lake was collected and the data revealed that the dominant members belong to the classes Bacillariophyceae and Zygnematophyceae, each having 15 species; followed by Chlorophyceae, Cyanophyceae and Euglenophyceae. Similarly, the physico-chemical parameters of water were measured by a digital portable multiparameter (HI98194) in the field. Water temperature was low during post-monsoon season. Electrical conductivity, Total Dissolved Solids (TDS), Oxidation Reduction Potential and Dissolved Oxygen were high in post-monsoon season while the pH of the lake water was alkaline and low pH in post-monsoon season. The number of algal species was found higher in pre-monsoon season than in post-monsoon season. The study showed that the lake is rich in algal species. Presence of the species like Closterium sp. and Oscillatoria sp. indicate that the lake is eutrophicated and polluted. Thus, further investigations were needed.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84381838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i2.56973
Babu Lal Tiruwa, Ashok Subedi, Rajkumar Gurung
The Annapurna Conservation Area (ACA) is the first and largest conservation area in Nepal and one of the richest protected areas of Nepal in terms of biodiversity. But the richness of the floristic diversity in ACA is not well documented. The present study was done through a review of the published and authentic works of literatures such as journals, books, reports to explore the diversity of vascular plants in the entire ACA. This study documented a total of 1,739 species of vascular plants belonging to 771 genera and 154 families, comprising 118 species of fern and fern allies, 16 species of Gymnosperms and 1,605 species of Angiosperms respectively. Asteraceae with 56 genera and 149 species was found to be the largest family, followed by Poaceae (61 genera, 91 species), Fabaceae (42 genera, 83 species), Rosaceae (23 genera, 82 species), Orchidaceae (43 genera, 81 species), Ranunculaceae (11 genera, 73 species) and Lamiceae (29 genera, 58 species) respectively. Similarly, Saxifraga was found to be the largest genera with 28 species followed by Primula (26 species), Potentilla (19 species), Pedicularis (18 species), Saussurea and Gentiana (17 species each) and Berberis (16 species) respectively. In terms of growth form (habits) of the species found in ACA, trees (11% species), shrubs (14% species), herbs (69% species) and climbers (6% species) respectively. Total 102 species of Angiosperms were found to be the Endemic to Nepal from ACA. The rich diversity of vascular plants reflects that ACA is the central point for wide range of compositions of eastern and western Himalayan floristic components.
{"title":"Floristic Diversity of Vascular Plants in Annapurna Conservation Area (ACA), Gandaki Province, Nepal","authors":"Babu Lal Tiruwa, Ashok Subedi, Rajkumar Gurung","doi":"10.3126/bdpr.v20i2.56973","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.56973","url":null,"abstract":"The Annapurna Conservation Area (ACA) is the first and largest conservation area in Nepal and one of the richest protected areas of Nepal in terms of biodiversity. But the richness of the floristic diversity in ACA is not well documented. The present study was done through a review of the published and authentic works of literatures such as journals, books, reports to explore the diversity of vascular plants in the entire ACA. This study documented a total of 1,739 species of vascular plants belonging to 771 genera and 154 families, comprising 118 species of fern and fern allies, 16 species of Gymnosperms and 1,605 species of Angiosperms respectively. Asteraceae with 56 genera and 149 species was found to be the largest family, followed by Poaceae (61 genera, 91 species), Fabaceae (42 genera, 83 species), Rosaceae (23 genera, 82 species), Orchidaceae (43 genera, 81 species), Ranunculaceae (11 genera, 73 species) and Lamiceae (29 genera, 58 species) respectively. Similarly, Saxifraga was found to be the largest genera with 28 species followed by Primula (26 species), Potentilla (19 species), Pedicularis (18 species), Saussurea and Gentiana (17 species each) and Berberis (16 species) respectively. In terms of growth form (habits) of the species found in ACA, trees (11% species), shrubs (14% species), herbs (69% species) and climbers (6% species) respectively. Total 102 species of Angiosperms were found to be the Endemic to Nepal from ACA. The rich diversity of vascular plants reflects that ACA is the central point for wide range of compositions of eastern and western Himalayan floristic components.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84690828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i01.56600
Chandrakala Thakur, Raghu Ram Parajuli, Prativa Budhathoki, S. Rajbhandary
This study explores the spore germination and formation of sporophyte of Tectaria coadunata (Wall. ex Hook. & Grev.) C.Chr from its spores. Tectaria coadunata is a perennial evergreen edible fern species belonging to the family Tectariaceae. It is locally known as kali niuro in Nepali language and it is a very common wild vegetable in Nepal. We carried out the successful propagation of Tectaria coadunata from spores using two propagating media: coco-peat and soil mixture (soil, sand and compost manure).The temperature recorded from the spore sown time to development of sporophyte phase was 18-27°C while the general florescent light regime for 14-16 per day was supplied. To keep the propagating media humid and moist it was regularly monitored and maintained. The research is now in acclimatize phase and success of this study will be shared soon. Moreover, the work is successful in laboratory under control condition of certain factors like light and moisture and it is under progress to exploit its capacity to get survived in natural field, and the findings could be used as an alternative method of raising the plant in semi-natural environment.
{"title":"Propagation of Tectaria coadunata (Wall. ex Hook. & Grev.) C.Chr by Spores","authors":"Chandrakala Thakur, Raghu Ram Parajuli, Prativa Budhathoki, S. Rajbhandary","doi":"10.3126/bdpr.v20i01.56600","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56600","url":null,"abstract":"This study explores the spore germination and formation of sporophyte of Tectaria coadunata (Wall. ex Hook. & Grev.) C.Chr from its spores. Tectaria coadunata is a perennial evergreen edible fern species belonging to the family Tectariaceae. It is locally known as kali niuro in Nepali language and it is a very common wild vegetable in Nepal. We carried out the successful propagation of Tectaria coadunata from spores using two propagating media: coco-peat and soil mixture (soil, sand and compost manure).The temperature recorded from the spore sown time to development of sporophyte phase was 18-27°C while the general florescent light regime for 14-16 per day was supplied. To keep the propagating media humid and moist it was regularly monitored and maintained. The research is now in acclimatize phase and success of this study will be shared soon. Moreover, the work is successful in laboratory under control condition of certain factors like light and moisture and it is under progress to exploit its capacity to get survived in natural field, and the findings could be used as an alternative method of raising the plant in semi-natural environment.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82943558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i01.56584
R. Khanal, S. Devkota, M. Devkota
In this study, we studied the pattern and relationship between tree species richness along an elevation gradient in the southern aspect of Modi River Basin, Annapurna Conservation Area (ACA), Central Nepal. Altogether, 30 quadrats were established at 15 elevation bands between 1000 m and 3800 m above the mean sea level. Thirty tree species belonging to 21 families were recorded; Fabaceae had the highest number of species (4 spp.) followed by Betulaceae (3 spp.) and Anacardiaceae (3 spp.). Canonical correspondence analysis (CCA) tool was applied which revealed that the species richness and distribution varied significantly along an elevation gradient. The bimodal pattern of species richness was observed. It is concluded that the higher the elevation level, the lesser the number of tree species, thus, the trees in study site eschewed higher elevation levels.
{"title":"Variation in Tree Species Richness along an Elevation Gradient in the Modi River Basin, Annapurna Conservation Area, Nepal","authors":"R. Khanal, S. Devkota, M. Devkota","doi":"10.3126/bdpr.v20i01.56584","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56584","url":null,"abstract":"In this study, we studied the pattern and relationship between tree species richness along an elevation gradient in the southern aspect of Modi River Basin, Annapurna Conservation Area (ACA), Central Nepal. Altogether, 30 quadrats were established at 15 elevation bands between 1000 m and 3800 m above the mean sea level. Thirty tree species belonging to 21 families were recorded; Fabaceae had the highest number of species (4 spp.) followed by Betulaceae (3 spp.) and Anacardiaceae (3 spp.). Canonical correspondence analysis (CCA) tool was applied which revealed that the species richness and distribution varied significantly along an elevation gradient. The bimodal pattern of species richness was observed. It is concluded that the higher the elevation level, the lesser the number of tree species, thus, the trees in study site eschewed higher elevation levels.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90540659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i2.57028
K. P. Sinkar, Piyush S. Bafna, R. Mutha
Out of different secondary metabolites, flavonoids attract the attention of researchers due to their pharmacological potential and health benefits. But solubility and bioavailability issues severely restrict their use. Development of nanoformulation of flavonoids is one of the solutions to overcome these issues. The purpose of this study was to develop and characterize chrysin (CHR) loaded nanoparticles (CHRNs) by nanoprecipitation technique with Eudragit® and polyvinyl alcohol (PVA) as carriers. Particle size, polydispersity index (PDI), zeta potential, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRPD) was used to characterize the prepared CHRNs. The present study shows that CHRNs can be fabricated by a nanoprecipitation technique using the optimum weight ratio of CHR: Eudragit: PVA (1: 5: 5). The particle size, PDI, and zeta potential were found to be 238.1 nm, 0.434 and -20.1 mV. According to FTIR, CHR developed intermolecular hydrogen bonds with polymers (carriers). SEM imaging confirmed roughly spherical type particles with size of 100–400 nm. The results from the XRPD of the CHRNs showed that the crystal of the drug might be converted to an amorphous state. The release of the drug from the CHRNs was 85.54% compared with the pure drug at 45.11%.
{"title":"Nanoprecipitation Based Preparation and Physicochemical Characterization of Flavonoid Nanoparticles","authors":"K. P. Sinkar, Piyush S. Bafna, R. Mutha","doi":"10.3126/bdpr.v20i2.57028","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57028","url":null,"abstract":"Out of different secondary metabolites, flavonoids attract the attention of researchers due to their pharmacological potential and health benefits. But solubility and bioavailability issues severely restrict their use. Development of nanoformulation of flavonoids is one of the solutions to overcome these issues. The purpose of this study was to develop and characterize chrysin (CHR) loaded nanoparticles (CHRNs) by nanoprecipitation technique with Eudragit® and polyvinyl alcohol (PVA) as carriers. Particle size, polydispersity index (PDI), zeta potential, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRPD) was used to characterize the prepared CHRNs. The present study shows that CHRNs can be fabricated by a nanoprecipitation technique using the optimum weight ratio of CHR: Eudragit: PVA (1: 5: 5). The particle size, PDI, and zeta potential were found to be 238.1 nm, 0.434 and -20.1 mV. According to FTIR, CHR developed intermolecular hydrogen bonds with polymers (carriers). SEM imaging confirmed roughly spherical type particles with size of 100–400 nm. The results from the XRPD of the CHRNs showed that the crystal of the drug might be converted to an amorphous state. The release of the drug from the CHRNs was 85.54% compared with the pure drug at 45.11%.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86141995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i2.57001
Madhu Shudan Thapa Magar, Seerjana Maharjan, Januka Pathak, Ganga Rijal
The genus Dendrobium, with sparse distribution in nature, is one of the largest genera of Orchidaceae. DNA barcoding could be the best option for rapid and accurate identification of the Dendrobium species. The objective of the present study is to delineate the Dendrobium species using DNA barcoding technology. Here, we used a specimen of Dendrobium sp. collected from Brindaban Botanical Garden, Makawanpur (540 m asl) as a test object. We amplified and sequenced three chloroplast loci, rbcL (Ribulose-1,5- bisphosphate carboxylase), matK (Maturase K) and psbA-trnH (intergenic spacer) from the specimen. We retrieved twelve accessions of plastome sequences from NCBI, representing six Dendrobium species (D. candidum, D. crepidatum, D. chrysanthum, D. denneanum, D. fimbriatum and D. moschatum) reported in Nepal. Similarly, one accession of plastome of Bulbophyllum epiphytum was also retrieved, to be used as an out-group. Respective aligned sequences of rbcL, matK and psbA-trnH were extracted from each accession. Evolutionary analysis was performed following the Maximum Likelihood approach using MEGA X. The result showed that the evolutionary tree generated with combined sequences of all three loci (rbcL, matK and psbA-trnH) was better compared to that generated with sequence of single locus. However, additional markers are required for higher accuracy.
{"title":"DNA Barcoding of Dendrobium moschatum (Banks) Sw. Specimen from Makawanpur, Central Nepal","authors":"Madhu Shudan Thapa Magar, Seerjana Maharjan, Januka Pathak, Ganga Rijal","doi":"10.3126/bdpr.v20i2.57001","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57001","url":null,"abstract":"The genus Dendrobium, with sparse distribution in nature, is one of the largest genera of Orchidaceae. DNA barcoding could be the best option for rapid and accurate identification of the Dendrobium species. The objective of the present study is to delineate the Dendrobium species using DNA barcoding technology. Here, we used a specimen of Dendrobium sp. collected from Brindaban Botanical Garden, Makawanpur (540 m asl) as a test object. We amplified and sequenced three chloroplast loci, rbcL (Ribulose-1,5- bisphosphate carboxylase), matK (Maturase K) and psbA-trnH (intergenic spacer) from the specimen. We retrieved twelve accessions of plastome sequences from NCBI, representing six Dendrobium species (D. candidum, D. crepidatum, D. chrysanthum, D. denneanum, D. fimbriatum and D. moschatum) reported in Nepal. Similarly, one accession of plastome of Bulbophyllum epiphytum was also retrieved, to be used as an out-group. Respective aligned sequences of rbcL, matK and psbA-trnH were extracted from each accession. Evolutionary analysis was performed following the Maximum Likelihood approach using MEGA X. The result showed that the evolutionary tree generated with combined sequences of all three loci (rbcL, matK and psbA-trnH) was better compared to that generated with sequence of single locus. However, additional markers are required for higher accuracy.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88736510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i2.57039
N. Joshi
Socio-culture plays a significant role in conservation of indigenous knowledge and ethnic food in Nepal. Ethnic food makes the basis of diversified food which contributes to improve the health, besides food and nutrition securities. Documentation of plant based traditional food is crucial to enhance intercultural and intergenerational relations. Most of the traditional foods are prepared to celebrate rituals and culture using plants and plant products. This study gives a general overview of plant based traditional foods and rituals of Newar ethnic group inhabiting in Kathmandu Valley, Central Nepal. Data were gathered through ethnobotanical inventory, through interviews, participatory observations at festivals and ceremonies to document plants type, types of traditional food, consumption of traditional food in rituals, in addition to the challenges involved for indigenous knowledge preservation. Altogether 54 plant species were used for preparation of 45 types of traditional food. These traditional foods are consumed during celebration of more than 25 rituals and cultures of Newar ethnic group. Wild plants such as Blumea lacera, Centella asiatica, Choerospondias axillaris, Urtica dioica, etc. in particular occurred close to the areas where Newar community live and are mostly used for traditional food preparation. Other important plants were supplied by cultivation (i.e., domesticated species) and through markets. The young generation showed little interest in the consumption of traditional food. There is a great challenge to conserve traditional food and the related culture. Documentation of traditional knowledge about ethnic foods and their consumption in rituals will contribute for sustainable conservation of traditional food and culture for future generations.
{"title":"Ethnobotanical Study of Traditional Food in Newar Community of Kathmandu Valley, Central Nepal","authors":"N. Joshi","doi":"10.3126/bdpr.v20i2.57039","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57039","url":null,"abstract":"Socio-culture plays a significant role in conservation of indigenous knowledge and ethnic food in Nepal. Ethnic food makes the basis of diversified food which contributes to improve the health, besides food and nutrition securities. Documentation of plant based traditional food is crucial to enhance intercultural and intergenerational relations. Most of the traditional foods are prepared to celebrate rituals and culture using plants and plant products. This study gives a general overview of plant based traditional foods and rituals of Newar ethnic group inhabiting in Kathmandu Valley, Central Nepal. Data were gathered through ethnobotanical inventory, through interviews, participatory observations at festivals and ceremonies to document plants type, types of traditional food, consumption of traditional food in rituals, in addition to the challenges involved for indigenous knowledge preservation. Altogether 54 plant species were used for preparation of 45 types of traditional food. These traditional foods are consumed during celebration of more than 25 rituals and cultures of Newar ethnic group. Wild plants such as Blumea lacera, Centella asiatica, Choerospondias axillaris, Urtica dioica, etc. in particular occurred close to the areas where Newar community live and are mostly used for traditional food preparation. Other important plants were supplied by cultivation (i.e., domesticated species) and through markets. The young generation showed little interest in the consumption of traditional food. There is a great challenge to conserve traditional food and the related culture. Documentation of traditional knowledge about ethnic foods and their consumption in rituals will contribute for sustainable conservation of traditional food and culture for future generations.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87677822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3126/bdpr.v20i2.56999
L. Joshi, P. Chalise
Pinus roxburghii Sarg. is a gymnosperm tree belonging to the family Pinaceae having extensive distribution in Bangladesh, Bhutan, China (Tibet), India, Nepal and Pakistan. In Nepal, it is found in subtropical region at an elevational range from 500 to 2700 m asl. The aim of the present study is to determine the ecological variation in wood characters and non-anatomical characters such as diameter at breast height (DBH), tree height and altitude. Nine wood block samples were collected from the outermost part of the trunk of three matured canopy trees at three different localities between 1100 and 1350 m above sea level from central Nepal. Correlation and regression analysis were carried out to study variation in wood anatomical characters. Multiple regression analysis was done using non-anatomical factors as independent variables and anatomical features as dependent variables. Quantitative wood characters were found to be affected by change in ecological factors but not on its qualitative wood characters. Tracheids length as well as tracheid’s pit significantly varies with altitude. A weak correlation was found between wood anatomical characters and non-anatomical parameters. Tracheids length exhibited negative correlation whereas tracheids pit exhibits positive correlation with altitude. Since tracheids are the main conducting tissues in xylem of pines, decreasing length and increasing pit size of tracheids is of ecological importance. This study will help in identifying how wood characters are adapted in response to change in ecological factors as well as help to predict ecological factor disturbances.
{"title":"Ecological Wood Anatomy of Pinus roxburghii in Central Nepal","authors":"L. Joshi, P. Chalise","doi":"10.3126/bdpr.v20i2.56999","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.56999","url":null,"abstract":"Pinus roxburghii Sarg. is a gymnosperm tree belonging to the family Pinaceae having extensive distribution in Bangladesh, Bhutan, China (Tibet), India, Nepal and Pakistan. In Nepal, it is found in subtropical region at an elevational range from 500 to 2700 m asl. The aim of the present study is to determine the ecological variation in wood characters and non-anatomical characters such as diameter at breast height (DBH), tree height and altitude. Nine wood block samples were collected from the outermost part of the trunk of three matured canopy trees at three different localities between 1100 and 1350 m above sea level from central Nepal. Correlation and regression analysis were carried out to study variation in wood anatomical characters. Multiple regression analysis was done using non-anatomical factors as independent variables and anatomical features as dependent variables. Quantitative wood characters were found to be affected by change in ecological factors but not on its qualitative wood characters. Tracheids length as well as tracheid’s pit significantly varies with altitude. A weak correlation was found between wood anatomical characters and non-anatomical parameters. Tracheids length exhibited negative correlation whereas tracheids pit exhibits positive correlation with altitude. Since tracheids are the main conducting tissues in xylem of pines, decreasing length and increasing pit size of tracheids is of ecological importance. This study will help in identifying how wood characters are adapted in response to change in ecological factors as well as help to predict ecological factor disturbances.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83944808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seed banks are the important means for the ex-situ conservation of plant genetic diversity. Though the seeds of domesticated varieties have been preserved for a long time, wild seed conservation is a noble concept in Nepal. Seed Bank of National Botanical Garden, Godawari, Lalitpur (NBG) is a major initiative for the conservation of wild plant seeds in Nepal. In the present study, a standardized seed banking procedures practiced at the Seed Bank of NBG, from seed collection to storage including germination test has been included. Currently, seeds of 84 wild species belonging to 77 genera of 47 families are preserved in the NBG. Alongside preserving wild genetic resources, NBG Seed Bank also provides materials for restoration, reintroduction and research that supports broader plant conservation goals, as well as contributes to education and public awareness about plant conservation.
{"title":"Wild Seed Conservation in National Botanical Garden, Lalitpur, Nepal","authors":"Tulasa Chaudhary, Manisha Nagarkoti, Sudhir Neupane, D. Lamichhane, Jeevan Pandey, Gaurav Parmar","doi":"10.3126/bdpr.v20i2.56992","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.56992","url":null,"abstract":"Seed banks are the important means for the ex-situ conservation of plant genetic diversity. Though the seeds of domesticated varieties have been preserved for a long time, wild seed conservation is a noble concept in Nepal. Seed Bank of National Botanical Garden, Godawari, Lalitpur (NBG) is a major initiative for the conservation of wild plant seeds in Nepal. In the present study, a standardized seed banking procedures practiced at the Seed Bank of NBG, from seed collection to storage including germination test has been included. Currently, seeds of 84 wild species belonging to 77 genera of 47 families are preserved in the NBG. Alongside preserving wild genetic resources, NBG Seed Bank also provides materials for restoration, reintroduction and research that supports broader plant conservation goals, as well as contributes to education and public awareness about plant conservation.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89842521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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