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mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks. 鸡甲基化相关酶mRNA表达与热调节持续时间的关系。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0210029
Yoshimitsu Ouchi, Vishwajit S Chowdhury, Takashi Bungo

DNA methylation regulates gene expression by modifying the nucleosome structure of DNA, without altering the gene sequence. It has been reported that DNA methylation reactions are catalyzed by several enzymes. In chickens, thermal conditioning treatment affects the central DNA methylation levels. The purpose of this study was to clarify the changes in DNA methylation and demethylation factors during thermal conditioning in the hypothalamus of 3-day-old chicks. Male chicks (3-days old) were exposed to 40±0.5°C as a thermal conditioning treatment for 1, 2, 6, 9, or 12 h. The control chicks were kept in a thermoneutral zone (30±0.2°C). After thermal conditioning, the mRNA levels of DNA methyltransferase (DNMT)-1, -3a, -3b, and ten-eleven translocation (TET)-1, -2, and -3 in the hypothalamus were measured by q-PCR. The mRNA levels of DNMT-3a and TET-1 were increased by thermal conditioning. Moreover, the expression level of TET-1 increased with the loading time of the thermal conditioning. The gene expressions of DNMT-1, DNMT-3b, TET-2, and TET-3 were not affected by thermal conditioning. Since DNMT-3a is a catalyst for de-novo DNA methylation and TET-1 catalyzes the oxidation of methylated cytosine, it is suggested that the thermal conditioning increased the activation of DNA methylation and demethylation factors, which occur in the hypothalamus of neonatal chicks.

DNA甲基化通过改变DNA的核小体结构来调节基因表达,而不改变基因序列。据报道,DNA甲基化反应是由几种酶催化的。在鸡中,热调节处理影响中心DNA甲基化水平。本研究旨在阐明3日龄雏鸡下丘脑热调节过程中DNA甲基化和去甲基化因子的变化。雄性雏鸡(3日龄)分别在40±0.5°C的温度下进行1、2、6、9和12小时的热调节处理。对照雏鸡在30±0.2°C的温度中性区。热调节后,采用q-PCR法检测下丘脑DNA甲基转移酶(DNMT)-1、-3a、-3b和TET -1、-2、-3 mRNA水平。热调节使DNMT-3a和TET-1 mRNA水平升高。TET-1的表达量随热调节加载时间的延长而增加。DNMT-1、DNMT-3b、TET-2和TET-3基因表达不受热调节的影响。由于DNMT-3a是脱氧DNA甲基化的催化剂,而TET-1可以催化甲基化胞嘧啶的氧化,因此,热调节增加了新生雏鸡下丘脑中DNA甲基化和去甲基化因子的激活。
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引用次数: 0
Evaluation of Commercial 6-Phytases on Growth Performance, Bone Mineral Content, and Feed Digestibility of Broiler Chicks. 商品6-植酸酶对肉仔鸡生长性能、骨矿物质含量和饲料消化率的影响
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0210010
Maki Shimeno, Yasushi Hashimoto, Aya Yanagawa, Mai Yamamoto, Teruhiro Yorozuya, Lode Nollet, Chisato Yonemochi

We evaluated the effects of 6-phytases on the growth performance of broilers (UK Chunky) under the recommended supplier-application dosage of each phytase. A nutritionally sufficient standard diet was administered as the base diets in the positive control feed. The diet in the negative control feed was designed by reducing total phosphorous, non-phytate phosphorus, and calcium by 0.1% to evaluate the effect of the nutrient restriction on broilers. Four 6-phytases were added to negative control feeds at the level of the recommended dosage of each product to compare the effect of phytases on broiler technical performance, tibia ash, and feed digestibility. Nine hundred one-day-old broiler chicks (males and females) were distributed in a completely randomized design composed of six treatments and three replicates of 50 chicks each. Chicks were fed ad libitum for 49 days. Body weight gain and feed intake were recorded on days 21 and 49, tibia ash was measured on day 21, and apparent ileal digestibility of dry matter, crude protein, and total phosphorus were analyzed on day 49. Birds reared with test feeds supplemented with phytase showed higher body weight gain and feed intake compared to those of the negative control birds. No significant differences in traits were observed among different phytase treatments. Similarly, the percentage of tibia ash increased when phytase was supplemented, resulting in higher bone levels compared to that of the positive control. The apparent ileal digestibility of crude protein and total phosphorus was enhanced by supplementing negative control diets with phytases.

研究了6-植酸酶在推荐供试剂量下对肉仔鸡生长性能的影响。阳性对照饲料以营养充足的标准日粮作为基础日粮。负对照饲粮通过降低0.1%的总磷、非植酸磷和钙来评价营养限制对肉仔鸡的影响。在阴性对照饲料中按推荐用量水平添加4种6-植酸酶,比较植酸酶对肉鸡技术性能、胫骨灰分和饲料消化率的影响。试验选用1日龄肉鸡900只(公母),采用完全随机设计,分为6个处理,3个重复,每个重复50只鸡。雏鸡自由饲喂49 d。第21天和第49天分别记录体增重和采食量,第21天测定胫骨灰分,第49天测定干物质、粗蛋白质和总磷的表观回肠消化率。试验饲料中添加植酸酶的雏鸟的增重和采食量均高于阴性对照。不同植酸酶处理间各性状无显著差异。同样,当补充植酸酶时,胫骨灰分的百分比增加,导致骨骼水平高于阳性对照。阴性对照饲粮中添加植酸酶可提高粗蛋白质和总磷的表观回肠消化率。
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引用次数: 0
Effects of Coated Trace Minerals and the Fat Source on Growth Performance, Antioxidant Status, and Meat Quality in Broiler Chickens. 微量元素涂层和脂肪源对肉鸡生长性能、抗氧化状态和肉质的影响
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0200108
Dafei Yin, Tiejin Tong, Amy F Moss, Ruiyang Zhang, Yinggu Kuang, Yong Zhang, Fangfang Li, Yujing Zhu

Inorganic trace minerals may exacerbate lipid peroxidation, thereby impacting lipid metabolism. This study aimed to compare the effects of inorganic and coated trace minerals in diets with different fat sources, on the performance, slaughter characteristics, and antioxidant status of broiler chickens. A total of 576 21-day-old Abor Acres broiler birds were randomly divided into four dietary treatment groups in a 2 (non-coated and coated trace minerals)×2 (soybean oil and lard) factorial design. Each treatment was replicated 12 times (12 birds per replicate). The results showed that coated minerals significantly improved the average daily gain (ADG) in weight and the feed conversion ratio (P<0.01), increased serum iron, zinc, selenium, and thyroxine contents, increased the activities of glutathione peroxidase, superoxide dismutase, total antioxidant capacity, and lipoprotein lipase (P<0.05), and decreased the serum and muscle malondialdehyde (MDA) contents (P<0.01). The use of soybean oil as the fat source resulted in a high ADG in weight, a low F/G ratio, reduced serum MDA content, and drip loss of breast and leg muscles (P<0.05). In conclusion, the supplementation of coated trace minerals improved growth performance, antioxidant status, trace mineral retention within serum, and lipid metabolism. Additionally, soybean oil also improved the growth performance, antioxidant performance, and meat quality of broilers. The combination of coated trace minerals and soybean oil generated the best growth performance, antioxidant status, and meat quality characteristics.

无机微量元素可能会加剧脂质过氧化,从而影响脂质代谢。本研究旨在比较不同脂肪来源日粮中无机微量矿物质和涂层微量矿物质对肉鸡生产性能、屠宰特征和抗氧化状态的影响。按照 2(无涂层和涂层微量元素)×2(大豆油和猪油)的因子设计,将 576 只 21 日龄的 Abor Acres 肉鸡随机分为四个日粮处理组。每个处理重复 12 次(每个重复 12 只鸡)。结果表明,添加了微量矿物质的日增重(ADG)和饲料转化率(PPPP
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引用次数: 0
Bitter Taste Perception in Chickens. 鸡的苦味感知。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0210017
Fuminori Kawabata, Shoji Tabata

Many behavioral studies and histological analyses of the sense of taste have been conducted in chickens, as it plays an important role in the ingestion of feed. In recent years, various taste receptors have been analyzed, and the functions of fatty acids, umami, and bitter taste receptors in chickens have become clear. In this review, the bitter taste sense in chickens, which is the taste quality by which animals reject poisons, is discussed among a variety of taste qualities. Chickens have taste buds in the palate, the base of the oral cavity, and the root of the tongue. Bitter taste receptors, taste receptor type 2 members 1, 2, and 7 (T2R1, T2R2, and T2R7) are expressed in these tissues. According to functional analyses of bitter taste receptors and behavioral studies, T2R1 and T2R7 are thought to be especially involved in the rejection of bitter compounds in chickens. Furthermore, the antagonists of these two functional bitter taste receptors were also identified, and it is expected that such antagonists will be useful in improving the taste quality of feed materials and poultry drugs that have a bitter taste. Bitter taste receptors are also expressed in extra-oral tissues, and it has been suggested that gastrointestinal bitter taste receptors may be involved in the secretion of gastrointestinal hormones and pathogen defense mechanisms. Thus, bitter taste receptors in chickens are suspected to play major roles in taste sensing and other physiological systems.

鸡的味觉在饲料的摄取中起着重要的作用,因此对鸡的味觉进行了许多行为学研究和组织学分析。近年来,人们对各种味觉感受器进行了分析,明确了鸡体内脂肪酸、鲜味和苦味感受器的功能。本文就鸡的苦味这一动物拒斥毒物的味觉品质,在众多的味觉品质中进行了讨论。鸡的味蕾长在上颚、口腔底部和舌根。苦味受体,味觉受体2型成员1、2和7 (T2R1、T2R2和T2R7)在这些组织中表达。根据苦味受体的功能分析和行为研究,T2R1和T2R7被认为特别参与鸡对苦味化合物的排斥反应。此外,我们还鉴定了这两种功能性苦味受体的拮抗剂,预计这两种拮抗剂将有助于改善具有苦味的饲料材料和家禽药物的口感质量。口腔外组织中也有苦味受体的表达,研究表明,胃肠道苦味受体可能参与了胃肠道激素的分泌和病原体的防御机制。因此,鸡的苦味感受器被怀疑在味觉感知和其他生理系统中发挥重要作用。
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引用次数: 3
Characteristics of Essential Oils of Apiaceae Family: Their Chemical Compositions, in vitro Properties and Effects on Broiler Production. 芹科植物精油的特性:它们的化学成分、体外特性和对肉鸡生产的影响
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0210042
Usman Ali, Saima Naveed, Shafqat Nawaz Qaisrani, Athar Mahmud, Zafar Hayat, Muhammad Abdullah, Motoi Kikusato, Masaaki Toyomizu

There has been an upsurge of interest in the phytobiotics coincident with the onset of the potential ban on the use of antibiotic growth promoters (AGPs) in the broiler industry and because many kinds of nutraceuticals play an important role in improving growth performance, feed efficiency, and gut health of broilers. In the previous years, significant biological activities of essential oils (EOs) belonging to phytobiotics were observed, including anti-bacterial, antifungal, antiviral, and antioxidant properties. We found new perspectives on the roles of EOs, particularly extracts from the Apiaceae family, which is one of the largest plant families, in potential replacement of AGPs, and on the chemical composition involved in regulating microorganism activity and oxidative damage. Furthermore, the positive effects of EOs on broiler production and the possible mechanisms inducing the involvement of gut health and growth performance have been studied.

随着肉鸡行业可能禁止使用抗生素生长促进剂(AGPs),人们对植物生物制剂的兴趣急剧上升,因为许多营养保健品在改善肉鸡的生长性能、饲料效率和肠道健康方面发挥着重要作用。前些年,人们观察到属于植物生物制剂的精油(EOs)具有显著的生物活性,包括抗菌、抗真菌、抗病毒和抗氧化特性。我们发现了 EO(特别是从最大的植物家族之一繖形花科植物中提取的精华)在潜在替代 AGPs 方面的作用,以及参与调节微生物活性和氧化损伤的化学成分的新视角。此外,还研究了环氧乙烷对肉鸡生产的积极影响,以及肠道健康和生长性能的可能诱导机制。
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引用次数: 0
Effects of Supplementation with Dried Neem Leaf Extract on Lipid Peroxidation and Antioxidant Enzyme mRNA Expression in the Pectoralis Major Muscle of Broiler Chickens. 添加干印度楝叶提取物对肉仔鸡胸大肌脂质过氧化和抗氧化酶mRNA表达的影响。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2022-01-25 DOI: 10.2141/jpsa.0200120
Kiriko Nakamura, Mitsuki Shishido, Saki Shimamoto, Goichiro Ogawa, Nikhil Khandelwal, Kenji Tatsugawa, Yoshikazu Fujita, Akira Ohtsuka, Daichi Ijiri

This study was conducted to evaluate the effects of dietary supplementation of dried neem (Azadirachta indica) leaf extract (DNE) on lipid peroxidation and the expression of genes encoding mRNAs in antioxidant enzymes in the pectoralis major muscle of chickens. A total of 24 male broiler chickens (ROSS308) were divided into three groups (n=8) at 21 days of age. The control group of chickens was fed a basal diet, and the remaining two groups of chickens were fed a basal diet supplemented with DNE at a concentration of 0.5% or 2.0% until 35 days of age. Growth performance (body weight, weight gain, feed intake, and feed conversion ratio) and tissue weights did not differ among the three groups. The 2.0% DNE-supplemented diet decreased the muscle malondialdehyde content, a marker of lipid peroxidation, and drip loss compared to the control chickens. In addition, the expression of genes encoding mRNAs of antioxidant enzymes (i.e., Cu/Zn-superoxide dismutase, Mn-superoxide dismutase, glutathione peroxidase 7, and catalase) were higher in the pectoralis major muscle of chickens fed the 2.0% DNE-supplemented diet than in the control chickens. Therefore, DNE supplementation increased the expression of genes encoding mRNAs in antioxidant enzymes and reduced lipid peroxidation and drip loss in the pectoralis major muscle of broiler chickens.

本试验旨在研究饲粮中添加印楝叶提取物(DNE)对鸡胸大肌脂质过氧化和抗氧化酶mrna编码基因表达的影响。选取24只21日龄的ROSS308肉鸡雄性,随机分为3组(n=8)。对照组饲喂基础饲粮,其余2组分别饲喂在基础饲粮中添加0.5%或2.0% DNE的试验饲粮,直至35日龄。生长性能(体重、增重、采食量和饲料系数)和组织质量在三组间无显著差异。与对照鸡相比,添加2.0% dne的饲粮降低了肌肉丙二醛含量(脂质过氧化指标)和滴漏损失。此外,饲粮中添加2.0% dne的鸡胸大肌中抗氧化酶(Cu/ zn -超氧化物歧化酶、mn -超氧化物歧化酶、谷胱甘肽过氧化物酶7和过氧化氢酶)mrna编码基因的表达量高于对照组。因此,添加DNE增加了肉仔鸡胸大肌抗氧化酶mrna编码基因的表达,减少了胸大肌脂质过氧化和滴水损失。
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引用次数: 3
Probiotic Bacillus subtilis C-3102 Improves Eggshell Quality after Forced Molting in Aged Laying Hens. 益生菌枯草芽孢杆菌 C-3102 改善老龄蛋鸡强制蜕皮后的蛋壳质量
IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2021-10-25 DOI: 10.2141/jpsa.0200081
Toki Nishiyama, Koichi Nakagawa, Tomokazu Imabayashi, Shun Iwatani, Naoyuki Yamamoto, Nobumichi Tsushima

This study was carried out to evaluate the effects of probiotic Bacillus subtilis C-3102 feed additive on quality characteristics including strength, thickness, and weight of eggshells of Boris Brown laying hens. The control group (n=64) was fed a basal diet comprised of maize and feed rice, whereas the experimental group (n=64) was fed a basal diet supplemented with B. subtilis C-3102 (3×105 CFU/g) starting at 49 weeks of age. From 67 to 69 weeks, all hens were induced to molt using an anorexic program; then, the birds in both groups returned to their respective diets (from 69 to 82 weeks). Eggshell strength, measured six times with 60 eggs selected before the molting treatment, was significantly greater in the C-3102 group than in the control group at 51, 59, 63, and 66 weeks (3.45, 3.44, 3.28, and 3.13 kg/cm2; P<0.05, 0.05, 0.01, and 0.01, respectively). Moreover, eggshell strength-measured three times after the molting treatment-was significantly greater in the C-3102 group than in the control group at 73 and 77 weeks (3.79 and 3.65 kg/cm2; P<0.01 and 0.01, respectively). Eggshell thickness was also significantly greater in the C-3102 group than in the control group at 73 and 77 weeks (0.400 and 0.390 mm; P<0.01 and 0.01, respectively). Fecal samples collected from eight hens of each group at 70 weeks of age after forced molting, showed a significantly higher proportion of Lactobacillus spp. in the C-3102 group (8.94 log CFU/g) (P<0.05) than in the control group (8.63 log CFU/g). Clostridium spp. abundance was significantly lower in the C-3102 group (2.92 log CFU/g) than in the control group (4.3 log CFU/g). These results suggest that C-3102 supplementation improves eggshell quality in aged laying hens, particularly after forced molting.

本研究旨在评估益生菌枯草芽孢杆菌 C-3102 饲料添加剂对波里斯褐蛋鸡蛋壳强度、厚度和重量等质量特性的影响。对照组(64 只)饲喂由玉米和饲料米组成的基础日粮,而实验组(64 只)从 49 周龄开始饲喂添加了枯草芽孢杆菌 C-3102 (3×105 CFU/g)的基础日粮。从 67 周到 69 周,所有母鸡都通过厌食程序诱导蜕皮;然后,两组的母鸡都回到各自的日粮中(从 69 周到 82 周)。在蜕皮处理前,用 60 枚鸡蛋测量了六次蛋壳强度,在 51、59、63 和 66 周时,C-3102 组的蛋壳强度显著高于对照组(3.45、3.44、3.28 和 3.13 公斤/枚)。C-3102组的梭状芽孢杆菌丰度(2.92 log CFU/g)明显低于对照组(4.3 log CFU/g)。这些结果表明,补充 C-3102 可改善老龄蛋鸡的蛋壳质量,尤其是在强制换羽后。
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引用次数: 0
KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media. kw -1比DMEM和199培养基更适合于鸡成纤维细胞培养。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2021-10-25 DOI: 10.2141/jpsa.0200085
Masafumi Katayama, Manabu Onuma, Tomokazu Fukuda

Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.

培养的细胞对家禽科学家来说是一个有用的资源,因为这些细胞使科学家能够在体外评估对传染病等条件的生物反应,同时模拟鸟类的全身反应。然而,禽类细胞培养需要一种优化的基础培养基,目前这种基础培养基的选择相对较少(培养基199和kw -1)。这意味着禽细胞培养的最佳基础培养基仍有发展的空间。在这里,我们比较了kw -1培养基、Dulbecco's modified Eagle培养基(DMEM)和199培养基在鸡成纤维细胞培养过程中的作用,并确定kw -1培养基仍然是这些试验的最佳培养基。我们的研究结果表明,与DMEM和medium 199相比,在kw -1培养基中培养的成纤维细胞的DNA损伤减少,并且与DMEM和medium 199相比,这些细胞在kw -1培养基中也表现出更好的生长动力学。据我们所知,这是第一个描述禽类细胞培养基比较分析的研究,这将为家禽科学家提供有用的信息。
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引用次数: 1
Screening of MicroRNAs with Potential Systemic Effects Released from Goose Fatty Liver. 鹅脂肪肝释放具有潜在全身效应的microrna的筛选。
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2021-10-25 DOI: 10.2141/jpsa.0200097
Xue Fan, Ya Xing, Long Liu, Chao Zhao, Zhenzhen Chen, Mawahib K Khogali, Minmeng Zhao, Xuming Hu, Hengmi Cui, Tuoyu Geng, Daoqing Gong

Communication between tissues and organs plays an important role in the maintenance of normal physiological functions as well as the occurrence and development of diseases. Communication molecules act as a bridge for interactions between tissues and organs, playing not only a local role in the tissues and organs where they are secreted but also in exerting systemic effects on the whole body via circulation. In this study, blood microRNA-omics analysis of overfed vs. normally fed (control) Landes geese revealed that the content of each of the 21 microRNAs (miRNAs) in the blood of overfed geese was significantly higher than that in the blood of control geese. These miRNAs may have systematic effects in the development of goose fatty liver as well as being candidate markers for the diagnosis of goose fatty liver. We determined the expression of miR-143, miR-455-5p, miR-222a-5p, miR-184, miR-1662, and miR-129-5p using quantitative PCR in goose fatty liver vs. that in normal liver. The expression of these miRNAs, except miR-129-5p, in goose fatty liver was also significantly higher than that in normal liver (P<0.05), suggesting that these blood miRNAs are released from goose fatty liver. In addition, we found that expression of IGFBP5, the predicted target gene of miR-143, was significantly decreased in goose fatty liver vs. the normal liver (P<0.05), indicating that miR-143 may exert both local and systematic effects by inhibiting the expression of IGFBP5, thus promoting the development of goose fatty liver. In conclusion, we identified several miRNAs, including those we validated (i.e., miR-143, miR-455-5p, miR-222a-5p, miR-184, miR-1662, and miR-129-5p) that may serve as candidate markers in the diagnosis of goose fatty liver as well as local and global regulators contributing to the development of goose fatty liver.

组织器官之间的通讯在维持正常的生理功能和疾病的发生发展中起着重要作用。通讯分子是组织和器官之间相互作用的桥梁,不仅在其分泌的组织和器官中发挥局部作用,而且通过循环对整个身体产生全身作用。本研究通过对过量饲养和正常饲养(对照)朗德鹅的血液microrna组学分析发现,过量饲养的朗德鹅血液中21种microrna (miRNAs)的含量均显著高于对照组。这些mirna可能在鹅脂肪肝的发展过程中具有系统性作用,也可能是鹅脂肪肝诊断的候选标记物。我们使用定量PCR检测了miR-143、miR-455-5p、miR-222a-5p、miR-184、miR-1662和miR-129-5p在鹅脂肪肝中的表达与在正常肝脏中的表达。除miR-129-5p外,这些mirna在鹅脂肪肝中的表达量也显著高于正常肝脏(miR-143的预测靶基因PIGFBP5在鹅脂肪肝中较正常肝脏显著降低)(PmiR-143可能通过抑制IGFBP5的表达而发挥局部和全身作用,从而促进鹅脂肪肝的发展。总之,我们确定了几个mirna,包括我们验证的mirna(即miR-143, miR-455-5p, miR-222a-5p, miR-184, miR-1662和miR-129-5p),它们可以作为鹅脂肪肝诊断的候选标记物,以及促进鹅脂肪肝发展的本地和全球调节因子。
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引用次数: 1
Population Structure and Genetic Diversity of Seven Chinese Indigenous Chicken Populations in Guizhou Province. 贵州省7个中国地方鸡种群结构与遗传多样性
IF 1.5 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2021-10-25 DOI: 10.2141/jpsa.0200060
Yifan Liu, Ming Zhang, Yunjie Tu, Jianmin Zou, Keyin Luo, Gaige Ji, Yanju Shan, Xiaojun Ju, Jingting Shu

To investigate the population structure and genetic diversity of indigenous chicken breeds in Guizhou, a total of 150 individual samples were collected from 12 breeds, including seven local chicken breeds in Guizhou Province, three Chinese native breeds found in other provinces, and two commercial breeds. The genotype datasets were obtained using a 50K single nucleotide polymorphism array method, and then a series of population analyses were performed. The obtained population parameters and linkage disequilibrium decay indicated a higher degree of genetic diversity in Guizhou chickens than in commercial breeds. Two Guizhou local breeds, Wumeng black-bone and Weining, were clustered with a breed from a neighboring province, Xinwen black-bone, which exhibited similar ancestral composition patterns. A newly found breed, Wumeng crested, had high genetic diversity and displayed genetic differences from other Guizhou breeds. These findings provide insight into the establishment of efficient conservation and utilization programs for Guizhou chicken breeds.

为调查贵州省地方鸡品种的种群结构和遗传多样性,共采集了12个品种的150个个体样本,其中包括7个贵州省地方鸡品种、3个外省中国地方鸡品种和2个商品鸡品种。采用50K单核苷酸多态性阵列法获得基因型数据集,并进行群体分析。所得群体参数和连锁不平衡衰减表明贵州鸡的遗传多样性程度高于商品品种。贵州乌蒙黑骨和威宁两个地方品种与邻省新文黑骨品种聚类,表现出相似的祖先组成模式。新发现的乌蒙冠毛品种具有较高的遗传多样性,与贵州其他品种存在遗传差异。研究结果可为贵州鸡种高效保护利用方案的制定提供参考。
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引用次数: 5
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Journal of Poultry Science
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