Journal of Reproductive Immunology最新文献

英文中文

NEUTROPHIL HETEROGENEITY AND FUNCTION IN EARLY PLACENTAL DEVELOPMENT: REGULATORS OF TROPHOBLAST INVASION?

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-02-01 DOI: 10.1016/j.jri.2024.104354

Z.N. Juvan , O. Kindler , T. Kloimboeck , AL. Hoebler , S. Vondra , A. Lackner , D. Feyaerts , C. Wadsack , B. Gaudilliere , J. Pollheimer , J. Kargl

引用次数: 0

DYSREGULATED IMMUNE CHECKPOINT INHIBITOR EXPRESSION IS EVIDENT IN WOMEN WITH CHRONIC HISTIOCYTIC INTERVILLOSITIS BEFORE THEY CONCEIVE

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-02-01 DOI: 10.1016/j.jri.2024.104338

E.F. Cornish * , M.A. Ivarsson , T.C.R. McDonnell , N.K. Björkström , D.J. Williams

引用次数: 0

The NLRP3 activation-related signature predicts the diagnosis and indicates immune characteristics in endometriosis

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-31 DOI: 10.1016/j.jri.2025.104443

Weihua Nong , Huimei Wei , Sheng Dou , Liqiao He , Luping Lin , Donglin Lu , Bixiao Wei , Shun Zhang , Peng Huo , Mingyou Dong

Endometriosis (EMS) is a prevalent gynecological disease that leads to chronic pelvic pain and infertility in women of reproductive age. However, the underlying pathogenic genes and effective treatment for EMS remain unclear. Therefore, this study aims to identify key genes influencing the diagnosis and treatment of EMS. The GSE7307 dataset, comprising 18 EMS and 23 control samples, was obtained from the GEO database. Fourteen differential genes related to NOD-like receptor protein 3 (NLRP3) activation and EMS were extracted from endometrial samples in GSE7307 through differential analysis. GO and KEGG analyses revealed that these genes were primarily involved in the production and regulation of the cytokine IL-1β and the NOD-like receptor signaling pathway. Random Forest (RF) and support vector machine recursive feature elimination algorithms were employed to select four diagnostic markers related to NLRP3 activation (NLRP3, IL-1β, LY96, and PDIA3) for constructing the EMS diagnostic model. These markers were validated using western blotting and tested in GSE7305 and GSE23339 datasets. The AUC values demonstrated the model's robust diagnostic performance. Additionally, the infiltration of immune cells in the samples and the correlation between different immune factors and diagnostic markers were explored. These results suggest that the four diagnostic markers may also play a crucial role in EMS immunity. Finally, the DrugBank database indicated that niclosamide could be effective for NLRP3-targeted therapy. In conclusion, we identified four key diagnostic genes for EMS, and niclosamide emerged as a potential drug for NLRP3-targeted therapy in EMS.

{"title":"The NLRP3 activation-related signature predicts the diagnosis and indicates immune characteristics in endometriosis","authors":"Weihua Nong , Huimei Wei , Sheng Dou , Liqiao He , Luping Lin , Donglin Lu , Bixiao Wei , Shun Zhang , Peng Huo , Mingyou Dong","doi":"10.1016/j.jri.2025.104443","DOIUrl":"10.1016/j.jri.2025.104443","url":null,"abstract":"<div><div>Endometriosis (EMS) is a prevalent gynecological disease that leads to chronic pelvic pain and infertility in women of reproductive age. However, the underlying pathogenic genes and effective treatment for EMS remain unclear. Therefore, this study aims to identify key genes influencing the diagnosis and treatment of EMS. The GSE7307 dataset, comprising 18 EMS and 23 control samples, was obtained from the GEO database. Fourteen differential genes related to NOD-like receptor protein 3 (NLRP3) activation and EMS were extracted from endometrial samples in GSE7307 through differential analysis. GO and KEGG analyses revealed that these genes were primarily involved in the production and regulation of the cytokine IL-1β and the NOD-like receptor signaling pathway. Random Forest (RF) and support vector machine recursive feature elimination algorithms were employed to select four diagnostic markers related to NLRP3 activation (NLRP3, IL-1β, LY96, and PDIA3) for constructing the EMS diagnostic model. These markers were validated using western blotting and tested in GSE7305 and GSE23339 datasets. The AUC values demonstrated the model's robust diagnostic performance. Additionally, the infiltration of immune cells in the samples and the correlation between different immune factors and diagnostic markers were explored. These results suggest that the four diagnostic markers may also play a crucial role in EMS immunity. Finally, the DrugBank database indicated that niclosamide could be effective for NLRP3-targeted therapy. In conclusion, we identified four key diagnostic genes for EMS, and niclosamide emerged as a potential drug for NLRP3-targeted therapy in EMS.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104443"},"PeriodicalIF":2.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of CXCL12/CXCR4 pair in migration of thymocytes from lactating mice

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-29 DOI: 10.1016/j.jri.2025.104444

Návylla Candeia de Medeiros , Elvan Nascimento dos Santos Filho , Davi Alexandre Silva Ayres , Bruno Henrique Mello Ayres Cancio , Salete Smaniotto , Maria Danielma dos Santos Reis , Marvin Paulo Lins

During the postpartum and lactation period, the involuted thymus, affected by pregnancy hormones, initiates its morphophysiological recovery with the positive regulation of the hormone prolactin (PRL), present at high levels during lactation. PRL has pleiotropic effects on thymic epithelial cells, thymocytes, as well as on elements of the extracellular matrix. In this study, we evaluated the production of the chemokine CXCL12 by the thymic microenvironment and its receptor, CXCR4, by thymocytes from lactating female mice. To achieve this objective, we used C57BL/6 female mice that were nulliparous or on the 15th day of lactation. After euthanasia, their thymi were collected, weighed, and processed for histological analyses and PRL quantitation. In other experiments, the thymus was mechanically disrupted to obtain fresh thymocytes, which were subsequently subjected to in vitro assays and flow cytometry analyses. Also, the plasma serum of the females was collected to measure PRL levels. It was observed that there was lower cellularity and thymic weight on the 15th day of lactation, along with histological alterations that this organ normally exhibits during this period. It was found that the thymic supernatant presented higher levels of PRL than the animals' serum. Additionally, the thymic medulla exhibited higher quantities of CXCL12, and thymocytes displayed elevated levels of CXCR4, making them more adept at migrating in response to this chemotactic stimulus. Remarkably, this is the first report, to the best of our knowledge, indicating the action of the CXCL12/CXCR4 pair in the thymus during the physiological lactation period.

{"title":"Involvement of CXCL12/CXCR4 pair in migration of thymocytes from lactating mice","authors":"Návylla Candeia de Medeiros , Elvan Nascimento dos Santos Filho , Davi Alexandre Silva Ayres , Bruno Henrique Mello Ayres Cancio , Salete Smaniotto , Maria Danielma dos Santos Reis , Marvin Paulo Lins","doi":"10.1016/j.jri.2025.104444","DOIUrl":"10.1016/j.jri.2025.104444","url":null,"abstract":"<div><div>During the postpartum and lactation period, the involuted thymus, affected by pregnancy hormones, initiates its morphophysiological recovery with the positive regulation of the hormone prolactin (PRL), present at high levels during lactation. PRL has pleiotropic effects on thymic epithelial cells, thymocytes, as well as on elements of the extracellular matrix. In this study, we evaluated the production of the chemokine CXCL12 by the thymic microenvironment and its receptor, CXCR4, by thymocytes from lactating female mice. To achieve this objective, we used C57BL/6 female mice that were nulliparous or on the 15th day of lactation. After euthanasia, their thymi were collected, weighed, and processed for histological analyses and PRL quantitation. In other experiments, the thymus was mechanically disrupted to obtain fresh thymocytes, which were subsequently subjected to <em>in vitro</em> assays and flow cytometry analyses. Also, the plasma serum of the females was collected to measure PRL levels. It was observed that there was lower cellularity and thymic weight on the 15th day of lactation, along with histological alterations that this organ normally exhibits during this period. It was found that the thymic supernatant presented higher levels of PRL than the animals' serum. Additionally, the thymic medulla exhibited higher quantities of CXCL12, and thymocytes displayed elevated levels of CXCR4, making them more adept at migrating in response to this chemotactic stimulus. Remarkably, this is the first report, to the best of our knowledge, indicating the action of the CXCL12/CXCR4 pair in the thymus during the physiological lactation period.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104444"},"PeriodicalIF":2.9,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does COVID-19 infection or COVID-19 mRNA vaccination induce antiphospholipid antibodies in women with recurrent pregnancy loss?

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-28 DOI: 10.1016/j.jri.2025.104442

Toshiyuki Takeshita, Naomi Nishimiya, Yachika Hihara

Antiphospholipid syndrome (APS) is a well-established cause of recurrent pregnancy loss (RPL). If coronavirus disease 2019 (COVID-19) infection or vaccination induces antiphospholipid antibody (aPL) production, it may lead to miscarriage in subsequent pregnancies. We investigated the association between COVID-19 infection and vaccination history with aPL positivity in women with RPL. This study included 424 women with RPL. We found no difference in the positivity rate for aPL according to the presence or absence of a history of COVID-19 infection. The positivity rate was significantly higher in patients infected during the omicron period (27.9 %, 43/154) than in those infected during the delta period (8.7 %, 2/23) of the COVID-19 pandemic (P = 0.0351). Of the 416 patients with a detailed vaccination history, 365 (87.7 %) had received at least one vaccination. The aPL positivity rate did not significantly differ according to the history of vaccination or number of vaccinations. Our results suggest that mild COVID-19 infection and vaccination are unlikely to stimulate aPL production and, therefore, are unlikely to increase miscarriage due to APS.

{"title":"Does COVID-19 infection or COVID-19 mRNA vaccination induce antiphospholipid antibodies in women with recurrent pregnancy loss?","authors":"Toshiyuki Takeshita, Naomi Nishimiya, Yachika Hihara","doi":"10.1016/j.jri.2025.104442","DOIUrl":"10.1016/j.jri.2025.104442","url":null,"abstract":"<div><div>Antiphospholipid syndrome (APS) is a well-established cause of recurrent pregnancy loss (RPL). If coronavirus disease 2019 (COVID-19) infection or vaccination induces antiphospholipid antibody (aPL) production, it may lead to miscarriage in subsequent pregnancies. We investigated the association between COVID-19 infection and vaccination history with aPL positivity in women with RPL. This study included 424 women with RPL. We found no difference in the positivity rate for aPL according to the presence or absence of a history of COVID-19 infection. The positivity rate was significantly higher in patients infected during the omicron period (27.9 %, 43/154) than in those infected during the delta period (8.7 %, 2/23) of the COVID-19 pandemic (<em>P</em> = 0.0351). Of the 416 patients with a detailed vaccination history, 365 (87.7 %) had received at least one vaccination. The aPL positivity rate did not significantly differ according to the history of vaccination or number of vaccinations. Our results suggest that mild COVID-19 infection and vaccination are unlikely to stimulate aPL production and, therefore, are unlikely to increase miscarriage due to APS.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104442"},"PeriodicalIF":2.9,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human embryo implantation: The complex interplay between endometrial receptivity and the microbiome

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-22 DOI: 10.1016/j.jri.2025.104440

Shiyang Peng , Amu Qingsan Meri , Min Zhou , Yuan Yu , Dongmei Tian , Shaomi Zhu

The endometrial and vaginal microbiota have co-evolved with the reproductive tract and play a key role in both health and disease. However, the difference between endometrial and vaginal microbiota, as well as their association with reproductive outcomes in women undergoing frozen embryo transfer, remains unclear. 120 women who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) and whole embryo freezing were enrolled. The vaginal and uterine microbiome were sequenced during the first frozen thawed embryo transfer. Based on whether or not they were pregnant after embryo transfer, women were assigned into two groups, and the microbiome of their reproductive tract was compared. The V3-V4 regions of the 16S rRNA gene were examined in the samples using the Next Generation Sequencing method. In the vagina, the non-pregnant group had higher bacterial species richness and diversity, with significantly lower Lactobacillus levels (91.66 % & 74.50 %) and higher Gardnerella levels (3.92 % & 12.12 %) than pregnant group (P < 0.05). In the uterine cavity, the diversity of uterine microbiome between pregnant group and non-pregnant group showed no significant differences. However, dramatic decrease in Lactobacillus (37.27 % & 33.45 %) and Pseudomonas (9.80 % & 4.08 %) were observed in the non-pregnant group (P < 0.05). There may be a correlation between the composition of female reproductive tract microbiome and the reproductive outcomes of patients with frozen-thawed embryo transfer. Lactobacillus-dominated microbiome in reproductive tract is more likely to be associated with higher clinical and ongoing pregnancy rate.

{"title":"Human embryo implantation: The complex interplay between endometrial receptivity and the microbiome","authors":"Shiyang Peng , Amu Qingsan Meri , Min Zhou , Yuan Yu , Dongmei Tian , Shaomi Zhu","doi":"10.1016/j.jri.2025.104440","DOIUrl":"10.1016/j.jri.2025.104440","url":null,"abstract":"<div><div>The endometrial and vaginal microbiota have co-evolved with the reproductive tract and play a key role in both health and disease. However, the difference between endometrial and vaginal microbiota, as well as their association with reproductive outcomes in women undergoing frozen embryo transfer, remains unclear. 120 women who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) and whole embryo freezing were enrolled. The vaginal and uterine microbiome were sequenced during the first frozen thawed embryo transfer. Based on whether or not they were pregnant after embryo transfer, women were assigned into two groups, and the microbiome of their reproductive tract was compared. The V3-V4 regions of the 16S rRNA gene were examined in the samples using the Next Generation Sequencing method. In the vagina, the non-pregnant group had higher bacterial species richness and diversity, with significantly lower <em>Lactobacillus</em> levels (91.66 % & 74.50 %) and higher <em>Gardnerella</em> levels (3.92 % & 12.12 %) than pregnant group (P < 0.05). In the uterine cavity, the diversity of uterine microbiome between pregnant group and non-pregnant group showed no significant differences. However, dramatic decrease in <em>Lactobacillus</em> (37.27 % & 33.45 %) and <em>Pseudomonas</em> (9.80 % & 4.08 %) were observed in the non-pregnant group (P < 0.05). There may be a correlation between the composition of female reproductive tract microbiome and the reproductive outcomes of patients with frozen-thawed embryo transfer. Lactobacillus-dominated microbiome in reproductive tract is more likely to be associated with higher clinical and ongoing pregnancy rate.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104440"},"PeriodicalIF":2.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cystatin C 3 (CST3) drives pathological progression in recurrent spontaneous abortion

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-21 DOI: 10.1016/j.jri.2025.104441

Meng Li , Hongfei Xie , Xuan Du

This study aimed to investigate the role of Cystatin C (CST3) in trophoblast cell (TBC) function and its contribution in the development of recurrent spontaneous abortion (RSA). We established an inbred RSA model by crossing CBA/J and DBA/2 mice. We investigated and compared expression levels of CST3 and pathological changes in decidual tissues from these RSA and normal pregnant mice. We next isolated TBCs from RSA mice and transfected them with CST3 overexpression or silencing vectors to assess alterations in cell proliferation, invasion, apoptosis, autophagy, oxidative stress and inflammatory stress responses. Results showed that CST3 expression was significantly elevated in RSA mice compared to normal pregnant mice, accompanied by edema, degeneration of decidual cells, and structural disorganization. CST3 overexpression in TBCs led to a significant reduction in cloning and invasion abilities, increased apoptosis, shortened G0-G1 phase and enhanced autophagy. Conversely, silencing CST3 reversed these cellular activities, promoting TBC activity and reducing apoptosis. Additionally, CST3 overexpression intensified inflammatory and oxidative stress in TBCs, whereas silencing CST3 alleviated these stress responses, further supporting its role in RSA progression. In conclusion, CST3 is upregulated in RSA and contributes to its progression by inhibiting TBC activity, while accelerating apoptosis and autophagy. These findings suggest that CST3 silencing may offer a novel therapeutic strategy to improve pregnancy outcomes in RSA by restoring TBC function and reducing apoptosis and stress responses.

{"title":"Cystatin C 3 (CST3) drives pathological progression in recurrent spontaneous abortion","authors":"Meng Li , Hongfei Xie , Xuan Du","doi":"10.1016/j.jri.2025.104441","DOIUrl":"10.1016/j.jri.2025.104441","url":null,"abstract":"<div><div>This study aimed to investigate the role of Cystatin C (CST3) in trophoblast cell (TBC) function and its contribution in the development of recurrent spontaneous abortion (RSA). We established an inbred RSA model by crossing CBA/J and DBA/2 mice. We investigated and compared expression levels of CST3 and pathological changes in decidual tissues from these RSA and normal pregnant mice. We next isolated TBCs from RSA mice and transfected them with CST3 overexpression or silencing vectors to assess alterations in cell proliferation, invasion, apoptosis, autophagy, oxidative stress and inflammatory stress responses. Results showed that CST3 expression was significantly elevated in RSA mice compared to normal pregnant mice, accompanied by edema, degeneration of decidual cells, and structural disorganization. CST3 overexpression in TBCs led to a significant reduction in cloning and invasion abilities, increased apoptosis, shortened G0-G1 phase and enhanced autophagy. Conversely, silencing CST3 reversed these cellular activities, promoting TBC activity and reducing apoptosis. Additionally, CST3 overexpression intensified inflammatory and oxidative stress in TBCs, whereas silencing CST3 alleviated these stress responses, further supporting its role in RSA progression. In conclusion, CST3 is upregulated in RSA and contributes to its progression by inhibiting TBC activity, while accelerating apoptosis and autophagy. These findings suggest that CST3 silencing may offer a novel therapeutic strategy to improve pregnancy outcomes in RSA by restoring TBC function and reducing apoptosis and stress responses.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104441"},"PeriodicalIF":2.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143263188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis and identification of potential biomarkers for dysfunctional uterine bleeding

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-16 DOI: 10.1016/j.jri.2025.104427

N. Zhang , Y. Liang , Y.Q. Meng , Y.C. Li , X. Lu , L. Li , T. Ye

Clinical evidence increasingly suggests that traditional treatments for dysfunctional uterine bleeding (DUB) have limited success. In this study, blood samples from 10 DUB patients and 10 healthy controls were collected for transcriptome sequencing. Then, the differentially expressed genes (DEGs) were screened and crossed with the DUB-related module genes to obtain the target genes. These target genes were analyzed for functional enrichment. Further, the biomarkers were screened by protein-protein interaction (PPI) analysis and analyzed by the gene set enrichment analysis (GSEA) and ingenuity pathway analysis (IPA). To explore the pathogenesis of DUB, immune microenvironment analyses were also performed. Potential drugs targeting these biomarkers were predicted for clinical treatment. The expression of these biomarkers was validated using quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that, a total of 754 target genes were found to be related to cell proliferation and senescence. Five biomarkers—CENPE, KIF11, PIK3R1, SMC3, and SMC4—were identified, all of which were down-regulated in the DUB group, and most of these findings were confirmed by qRT-PCR. Notably, CENPE expression showed a negative association with activated NK cells and a positive association with resting NK cells. In addition, 44 potential drugs were predicted for DUB treatment. In conclusion, five DUB biomarkers were identified, enhancing understanding of gene regulation in DUB and providing a theoretical foundation for clinical diagnosis and treatment.

{"title":"Analysis and identification of potential biomarkers for dysfunctional uterine bleeding","authors":"N. Zhang , Y. Liang , Y.Q. Meng , Y.C. Li , X. Lu , L. Li , T. Ye","doi":"10.1016/j.jri.2025.104427","DOIUrl":"10.1016/j.jri.2025.104427","url":null,"abstract":"<div><div>Clinical evidence increasingly suggests that traditional treatments for dysfunctional uterine bleeding (DUB) have limited success. In this study, blood samples from 10 DUB patients and 10 healthy controls were collected for transcriptome sequencing. Then, the differentially expressed genes (DEGs) were screened and crossed with the DUB-related module genes to obtain the target genes. These target genes were analyzed for functional enrichment. Further, the biomarkers were screened by protein-protein interaction (PPI) analysis and analyzed by the gene set enrichment analysis (GSEA) and ingenuity pathway analysis (IPA). To explore the pathogenesis of DUB, immune microenvironment analyses were also performed. Potential drugs targeting these biomarkers were predicted for clinical treatment. The expression of these biomarkers was validated using quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that, a total of 754 target genes were found to be related to cell proliferation and senescence. Five biomarkers—CENPE, KIF11, PIK3R1, SMC3, and SMC4—were identified, all of which were down-regulated in the DUB group, and most of these findings were confirmed by qRT-PCR. Notably, <em>CENPE</em> expression showed a negative association with activated NK cells and a positive association with resting NK cells. In addition, 44 potential drugs were predicted for DUB treatment. In conclusion, five DUB biomarkers were identified, enhancing understanding of gene regulation in DUB and providing a theoretical foundation for clinical diagnosis and treatment.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104427"},"PeriodicalIF":2.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Earlier pregnancies in nulliparous women with current father and lower risks for preeclampsia and low-birth weight newborns

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-16 DOI: 10.1016/j.jri.2025.104431

Veera Hurme , Reetta Honkanen , Katri Backman , Anne M. Karvonen , Pirkka Kirjavainen , Leea Keski-Nisula

New paternity has been related to placenta-associated complications in pregnancy. We evaluated whether a lack of earlier pregnancies or deliveries with a current father are associated with the pregnancy, prenatal, and early neonatal outcomes after controlling for the most common maternal confounders in prospective birth cohort study. An online questionnaire was used to survey 4459 pregnant women from the Kuopio Birth Cohort in their third trimester. The topics included their history of paternity in current and earlier pregnancies. Data were combined with prenatal, perinatal, and early neonatal information. A multivariable logistic regression analysis was performed to compare the possible associations between selected pregnancy and early neonatal outcomes with respect to paternal change. Pregnant women with changed partners had higher rates of smoking during pregnancy and hypertension before pregnancy. In the adjusted analysis, primigravidas and nulliparous multigravidas with different father had the highest risks for preeclampsia (adjusted odds ratios (aORs) 4.46 and 2.69, respectively), low birth weight (aORs 3.15 and 2.25), and smallness for gestational age (aORs 2.23 and 2.16) compared to the parous controls. Nulliparous women who had earlier pregnancies with the current father had less preeclampsia or gestational hypertension, as well as low birth weight (aOR 0.42, 95 % confidence interval (CI) 0.21–0.82 and aOR 0.26, 95 % CI 0.08–0.87, respectively) compared to other nulliparas. Among parous women, most of the pregnancy, obstetric, and early neonatal outcomes were similar in the adjusted analysis, regardless of new changes in paternity.

{"title":"Earlier pregnancies in nulliparous women with current father and lower risks for preeclampsia and low-birth weight newborns","authors":"Veera Hurme , Reetta Honkanen , Katri Backman , Anne M. Karvonen , Pirkka Kirjavainen , Leea Keski-Nisula","doi":"10.1016/j.jri.2025.104431","DOIUrl":"10.1016/j.jri.2025.104431","url":null,"abstract":"<div><div>New paternity has been related to placenta-associated complications in pregnancy. We evaluated whether a lack of earlier pregnancies or deliveries with a current father are associated with the pregnancy, prenatal, and early neonatal outcomes after controlling for the most common maternal confounders in prospective birth cohort study. An online questionnaire was used to survey 4459 pregnant women from the Kuopio Birth Cohort in their third trimester. The topics included their history of paternity in current and earlier pregnancies. Data were combined with prenatal, perinatal, and early neonatal information. A multivariable logistic regression analysis was performed to compare the possible associations between selected pregnancy and early neonatal outcomes with respect to paternal change. Pregnant women with changed partners had higher rates of smoking during pregnancy and hypertension before pregnancy. In the adjusted analysis, primigravidas and nulliparous multigravidas with different father had the highest risks for preeclampsia (adjusted odds ratios (aORs) 4.46 and 2.69, respectively), low birth weight (aORs 3.15 and 2.25), and smallness for gestational age (aORs 2.23 and 2.16) compared to the parous controls. Nulliparous women who had earlier pregnancies with the current father had less preeclampsia or gestational hypertension, as well as low birth weight (aOR 0.42, 95 % confidence interval (CI) 0.21–0.82 and aOR 0.26, 95 % CI 0.08–0.87, respectively) compared to other nulliparas. Among parous women, most of the pregnancy, obstetric, and early neonatal outcomes were similar in the adjusted analysis, regardless of new changes in paternity.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"168 ","pages":"Article 104431"},"PeriodicalIF":2.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of anti-phosphatidylserine/prothrombin antibodies with adverse in vitro fertilization outcomes

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2025-01-16 DOI: 10.1016/j.jri.2025.104429

Dongyan Liu , Yanshi Wang , Yu Zhang , Haoyu Wang , Wenjuan Tang , Xiaoyu Duan , Ru Wang , Meihong Hu , Fangting Lu , Joanne Kwak-Kim , Li Wu

Anti-phosphatidylserine/prothrombin antibodies (aPS/PT) are classified as non-criteria antiphospholipid antibodies (aPL), and are strongly associated with thrombosis and pregnancy complications linked to antiphospholipid syndrome (APS). This study aimed to investigate whether aPS/PT positivity is associated with adverse outcomes in vitro fertilization (IVF). The study included infertile women who tested positive aPS/PT and underwent IVF cycles, as well as infertile controls with pure tubal etiology. We compared the impact of aPS/PT on baseline and clinical characteristics, immune-related indicators, IVF laboratory metrics, and pregnancy outcomes. Women with aPS/PT exhibited lower numbers of retrieved oocytes, embryos, and both perfect and available embryos, as well as reduced rates of blastocyst formation. Furthermore, an imbalanced Th17/Treg ratio and significantly elevated serum IL-17A levels were observed in women with aPS/PT compared to controls. In conclusion, the presence of aPS/PT is associated with adverse IVF and pregnancy outcomes. Early screening for aPS/PT and appropriate consultation for couples undergoing IVF-ET should be considered. Additionally, specific immune and inflammatory mechanisms related to aPS/PT warrant further investigation.

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