Journal of Reproductive Immunology最新文献_第4页

Predicting ART outcomes: The role of ovarian RAS and VEGF in follicular fluid of dominant follicles 预测 ART 的结果：优势卵泡的卵泡液中卵巢 RAS 和 VEGF 的作用

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-11-15 DOI: 10.1016/j.jri.2024.104393

Pingyin Lee , Niwei Yan , Guoqing Fan , Xiaokun Hu , Qingyun Mai , Canquan Zhou , Yubin Li

As tissue and intracellular RAS have been reported in different organs and systems. there is local RAS in the ovary, called the ovarian renin–angiotensin system (OVRAS). In this study, we investigated the correlation between RAS (total renin, AngII, Ang1–7), vascular endothelial growth factor (VEGF) and E2 in dominant follicular fluid and ovarian reserve capacity and patient age. Moreover, we analyzed its predictive value for assisted reproductive technology (ART) related outcomes. We observed that the concentrations of VEGF in the follicular fluid of dominant follicles in the ≥ 38 year old group were markedly higher than those in the < 30 year old group (P < 0.05). Total renin and AngII levels were positively correlated with normal fertilization rate (P < 0.05). Ang1–7 levels were positively correlated with the number of mature oocytes, oocyte maturation rate and number of 2PN fertilized cells (P < 0.05). Expressions of VEGF were negatively correlated with number of 2PN fertilized cells, number of D3 embryos for blastocyst culture, number of blastocysts formed, number of available embryos and number of high-quality embryos (P < 0.05). Thus, the expressions of OVRAS (total renin, AngII, Ang1–7 and VEGF) in dominant follicular fluid are correlated with ART outcomes.

由于不同器官和系统的组织和细胞内RAS均有报道，卵巢中也存在局部RAS，即卵巢肾素-血管紧张素系统（OVRAS）。在这项研究中，我们调查了优势卵泡液中的 RAS（总肾素、AngII、Ang1-7）、血管内皮生长因子（VEGF）和 E2 与卵巢储备能力和患者年龄之间的相关性。此外，我们还分析了其对辅助生殖技术（ART）相关结果的预测价值。我们观察到，≥38 岁组优势卵泡的卵泡液中 VEGF 的浓度明显高于 < 30 岁组（P < 0.05）。总肾素和 AngII 水平与正常受精率呈正相关（P < 0.05）。Ang1-7水平与成熟卵母细胞数、卵母细胞成熟率和2PN受精细胞数呈正相关（P <0.05）。血管内皮生长因子的表达与 2PN 受精细胞数、用于囊胚培养的 D3 胚胎数、形成的囊胚数、可用胚胎数和优质胚胎数呈负相关（P < 0.05）。因此，优势卵泡液中 OVRAS（总肾素、AngII、Ang1-7 和血管内皮生长因子）的表达与 ART 结果相关。

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引用次数: 0

Analysis of the presence of natural killer cell subpopulations in preterm human milk: A first approach 分析早产儿母乳中存在的自然杀伤细胞亚群：第一种方法

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-11-15 DOI: 10.1016/j.jri.2024.104394

Mario Daniel Caba-Flores , María de la Soledad Lagunes-Castro , Aracely López-Monteon , Rubí Viveros-Contreras , Juan Gerardo Neme Kuri , David Huerta-Morales , Samantha Ponce Ramos , Edith Nava Bustos , Angel Ramos-Ligonio

Several immune cell populations are transferred to the newborn through breast milk, including natural killer (NK) cells, which are critical for innate defense and regulation of the immune response, especially in preterm infants. The aim of this study was to analyze the presence of NK cell subpopulations in different types of preterm breast milk. The study quantified the presence of NK cell subpopulations by flow cytometry using the relative expression of CD56 and CD16 markers in colostrum, transitional and mature milk samples from preterm mothers. Flow cytometry analysis revealed the presence of five NK cell subpopulations, but unlike those reported in peripheral blood, CD56^dimCD16⁺ and CD56^-CD16⁺ populations are predominantly present in preterm milk, only the CD56^brightCD16^dim population is increased in mature milk. Analysis of NK cell subpopulations in preterm milk revealed a pattern of NK cell presence in preterm breast milk with predominantly cytotoxic phenotypes in relation to CD16 marker expression.

多种免疫细胞群通过母乳转移到新生儿体内，其中包括自然杀伤（NK）细胞，它们对先天防御和免疫反应的调节至关重要，尤其是对早产儿而言。本研究旨在分析不同类型早产儿母乳中 NK 细胞亚群的存在情况。研究通过流式细胞术，利用早产儿母亲初乳、过渡乳和成熟乳样本中 CD56 和 CD16 标记的相对表达量，对 NK 细胞亚群的存在进行了量化。流式细胞术分析显示存在五种 NK 细胞亚群，但与外周血中的报道不同的是，CD56dimCD16+ 和 CD56-CD16+ 亚群主要存在于早产儿乳汁中，只有 CD56brightCD16dim 亚群在成熟乳汁中有所增加。对早产儿乳汁中 NK 细胞亚群的分析表明，早产儿乳汁中的 NK 细胞以细胞毒性表型为主，这与 CD16 标记的表达有关。

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引用次数: 0

CX3CL1 (Fractalkine): An important cytokine in physiological and pathological pregnancies CX3CL1（Fractalkine）：生理和病理妊娠中的一种重要细胞因子。

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-11-12 DOI: 10.1016/j.jri.2024.104392

Xianyang Hu , Xixi Huang , Tingxuan Yin , Jiajia Chen , Weijie Zhao , Min Yu , Lu Liu , Meirong Du

C-X3-C motif chemokine ligand 1 (CX3CL1), commonly known as Fractalkine, is an important chemokine with dual functions of chemotaxis and adhesion. It plays a pivotal role in a variety of physiological processes and pathological conditions, particularly in conjunction with its receptor, C-X3-C motif chemokine receptor 1 (CX3CR1). This review focuses on the expression and intricate regulatory mechanisms of CX3CL1 at the maternal-fetal interface, emphasizing its multifaceted role during pregnancy. CX3CL1 was detected in the trophoblast and decidua tissues, playing a crucial role in recruitment of immune cells, enhancing endometrial receptivity, and modulating trophoblast cell activities. Abnormal expression of CX3CL1 has been correlated with adverse pregnancy outcomes such as spontaneous abortion, gestational diabetes, preeclampsia, and preterm births. By elucidating the complex interplay of CX3CL1 at the maternal-fetal interface, this review aims to shed light on its potential roles in pregnancy-related complications.

C-X3-C motif趋化因子配体 1（CX3CL1），俗称 "小筋骨"，是一种重要的趋化因子，具有趋化和粘附的双重功能。它在多种生理过程和病理状态中发挥着关键作用，尤其是与其受体 C-X3-C motif 趋化因子受体 1（CX3CR1）共同发挥作用。本综述将重点讨论 CX3CL1 在母胎界面的表达和复杂的调控机制，强调其在孕期的多方面作用。CX3CL1在滋养层和蜕膜组织中被检测到，在免疫细胞的招募、增强子宫内膜的接受能力和调节滋养层细胞的活性方面发挥着至关重要的作用。CX3CL1 的异常表达与自然流产、妊娠糖尿病、子痫前期和早产等不良妊娠结局相关。通过阐明 CX3CL1 在母胎界面的复杂相互作用，本综述旨在阐明其在妊娠相关并发症中的潜在作用。

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引用次数: 0

Association between cellular immune and preeclampsia and preterm birth: A Mendelian randomization study 细胞免疫与先兆子痫和早产的关系：孟德尔随机研究

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-11-09 DOI: 10.1016/j.jri.2024.104391

Runfang Wang, Cuilian Liu, Xiaodan Liu, Li Liu, Yuange Xiao, Yan Huo

Emerging evidences have highlighted immune-inflammatory imbalances as a critical driver of the pathogenesis for preeclampsia (PE) and preterm birth (PB), but the impact of specific immune factors on the diseases is largely unknown. Our aim was to determine whether these immune cells are causally associated with the onset of PE or PB. Drawing on publicly available genetic data, we applied Mendelian randomization analysis to probe the causal link of 731 immune traits (7 panels: TBNK panel, Regulatory T cells panel, Maturation stages of T-cell panel, Dendritic cell panel, B-cell panel, Monocyte panel and Myeloid cell pane) with the risk of PE and PB. The inverse variance weighting method (IVW) acted as the primary analysis to estimate the validity of causality, and sensitivity analyses were conducted to assessment of heterogeneity and pleiotropy. After adjusting for P-values for FDR method, CD27 on CD24+ CD27+ B cell, CD80 on plasmacytoid Dendritic Cell, CD33+ HLA DR+ CD14dim Absolute Count, CD33+ HLA DR+ CD14- Absolute Count, CD33+ HLA DR+ Absolute Count were remarkably causally involved in increased risk of PE, while HLA DR on Dendritic Cell exerted a protective causation against PE (P_FDR < 0.05). Moreover, we determined that CD45 on CD33dim HLA DR- in myeloid cells decreased PB risk, whereas CD11b on Granulocytic Myeloid-Derived Suppressor Cells had the opposite effect on PB (F_FDR < 0.2). This study provided genetic evidence for causal relationships between immune cell traits and PE and PB, offering potential candidate immunophenotypes for future studies on the etiology of pregnancy complications.

越来越多的证据表明，免疫炎症失衡是子痫前期（PE）和早产（PB）发病机制的关键驱动因素，但特定免疫因素对这些疾病的影响在很大程度上还不为人所知。我们的目的是确定这些免疫细胞是否与子痫前期或早产的发病有因果关系。利用公开的遗传数据，我们采用孟德尔随机分析法探究了 731 个免疫特质（7 个面板：TBNK面板、调节性T细胞面板、T细胞成熟阶段面板、树突状细胞面板、B细胞面板、单核细胞面板和骨髓细胞面板）与 PE 和 PB 风险的因果关系。反方差加权法（IVW）是估计因果关系有效性的主要分析方法，并进行了敏感性分析以评估异质性和多义性。经FDR法调整P值后，CD24+ CD27+ B细胞上的CD27、浆细胞树突状细胞上的CD80、CD33+ HLA DR+ CD14dim绝对计数、CD33+ HLA DR+ CD14- 绝对计数、CD33+ HLA DR+ 绝对计数与PE风险增加有显著的因果关系，而树突状细胞上的HLA DR对PE有保护作用（PFDR < 0.05）。此外，我们还发现，髓系细胞中 CD33dim HLA DR- 上的 CD45 可降低 PE 风险，而粒细胞髓系衍生抑制细胞上的 CD11b 则对 PE 有相反的影响（FFDR < 0.2）。这项研究为免疫细胞特征与 PE 和 PB 之间的因果关系提供了遗传学证据，为今后研究妊娠并发症的病因提供了潜在的候选免疫表型。

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引用次数: 0

Estriol in formation of mononuclear cells tolerogenic features 雌三醇在形成单核细胞耐受性特征方面的作用。

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-11-08 DOI: 10.1016/j.jri.2024.104390

Irina Nekrasova, Sergei Shirshev

Estriol (E₃) is one of hormones whose synthesis is mainly associated with pregnancy. The hormone can also regulate immune cells functions. E₃ influence on monocyte indoleamine-2,3-dioxygenase (IDO1) activity and Treg and NK cells’ markers expression was investigated. LPS and IFN-γ stimulated IDO1 activity was augmented by Е₃ independently of the hormone doses. Both Е₃ concentrations increased CD4⁺Foxp3⁺ lymphocytes percentage. This hormonal effect was mediated by protein kinase A. CD16 expression upon NK cells was reduced as a result of Е₃ influence. The data indicated that Е₃ is one of the factors which maintained immune tolerance during pregnancy and protected fetus preservation.

雌三醇（E3）是一种激素，其合成主要与怀孕有关。这种激素还能调节免疫细胞的功能。本研究探讨了雌三醇对单核细胞吲哚胺-2,3-二氧化酶（IDO1）活性以及 Treg 和 NK 细胞标志物表达的影响。Е3能增强LPS和IFN-γ刺激的IDO1活性，而与激素剂量无关。两种浓度的Е3都增加了CD4+Foxp3+淋巴细胞的百分比。这种激素效应是由蛋白激酶 A 介导的。由于Е3 的影响，NK 细胞的 CD16 表达减少。这些数据表明，Е3是维持孕期免疫耐受和保护胎儿的因素之一。

引用次数: 0

Transcriptomics analysis of differential gene expression and immune and inflammatory response mechanisms in patients with typical and non-criteria obstetric antiphospholipid syndrome (OAPS and NC-OAPS) 典型和非标准产科抗磷脂综合征（OAPS 和 NC-OAPS）患者不同基因表达及免疫和炎症反应机制的转录组学分析。

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-11-07 DOI: 10.1016/j.jri.2024.104389

Xuan Qi , Peng Liu , Yingjie Zhou , Lingyan Lei , Guoyu Xue , Ronghua Wang , Junping Wang , Huifang Guo

In this study, we investigated the molecular differences between patients with typical obstetric antiphospholipid syndrome (OAPS) and patients with non-criteria obstetric antiphospholipid syndrome (NC-OAPS) patients through transcriptome sequencing of peripheral blood samples from ten OAPS patients and ten NC-OAPS patients. Differentially expressed genes (DEGs) were identified, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, protein-protein interaction (PPI) analysis, and competitive endogenous RNA (ceRNA) network construction to identify hub genes. Verification was performed via Quantitative Real-time PCR (qPCR) in OAPS (n=9) and NC-OAPS (n=12) samples. We identified 240 DEGs in two groups. GO and KEGG analyses reviewed upregulated in pathways related to the inflammatory response; immune response; antigen processing and presentation; Th1, Th2, and Th17 cell differentiation; and NK cell-mediated cytotoxicity in OAPS patients. PPI and ceRNA network analyses identified key genes, with significant upregulation of CXCR2, JAK2, and MPO found in the OAPS group, which correlated with severe inflammation, JAK-STAT pathway activation, and increased NET activity in neutrophils. Other genes such as CD4, IL2RB, and NKG7, are involved in T-cell and NK cell regulation. Our results indicate enhanced inflammatory and immune responses in OAPS patients, suggesting more severe immune activity than in NC-OAPS patients, providing a basis for precise diagnostic and therapeutic strategies.

在这项研究中，我们通过对10名产科抗磷脂综合征（OAPS）患者和10名非标准产科抗磷脂综合征（NC-OAPS）患者的外周血样本进行转录组测序，研究了典型产科抗磷脂综合征（OAPS）患者和非标准产科抗磷脂综合征（NC-OAPS）患者之间的分子差异。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析、蛋白-蛋白相互作用（PPI）分析以及竞争性内源性 RNA（ceRNA）网络构建，确定了差异表达基因（DEGs），进而确定了枢纽基因。通过定量实时 PCR（qPCR）对 OAPS（n=9）和 NC-OAPS（n=12）样本进行验证。我们在两组样本中发现了 240 个 DEGs。GO 和 KEGG 分析审查了 OAPS 患者中与炎症反应、免疫反应、抗原处理和呈递、Th1、Th2 和 Th17 细胞分化以及 NK 细胞介导的细胞毒性相关的通路的上调情况。PPI和ceRNA网络分析确定了关键基因，在OAPS组中发现CXCR2、JAK2和MPO显著上调，这与严重炎症、JAK-STAT通路激活和中性粒细胞NET活性增加有关。其他基因，如 CD4、IL2RB 和 NKG7，参与了 T 细胞和 NK 细胞的调节。我们的研究结果表明，OAPS 患者的炎症和免疫反应增强，表明其免疫活动比 NC-OAPS 患者更为严重，这为精确诊断和治疗策略提供了依据。

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引用次数: 0

Increased circulating levels of novel anti-inflammatory cytokines IL-27 and IL-38 are associated with immunoendrocrine dysregulation and altered redox stress in polycystic ovarian syndrome 新型抗炎细胞因子 IL-27 和 IL-38 循环水平的升高与多囊卵巢综合征的免疫内分泌失调和氧化还原压力改变有关

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-10-31 DOI: 10.1016/j.jri.2024.104388

Sugumar Shruthi , Veerasamy Nirmaladevi , Vivekanandhan Aravindhan

Background

Polycystic ovary syndrome (PCOS) is a common metabolic/ endocrine disorder seen predominantly in women in their reproductive age, which increases the risk of infertility, endometrial cancer and metabolic disorders. IL-27 and IL-38 are recently discovered, novel anti-inflammatory cytokines whose role in immune-endocrine dysfunction seen in PCOS is largely unknown.

Methods

In the present study, we quantified these two cytokines along with markers for meta-inflammation (TNF-α, IL-6, IL-1β, IL-1Ra, IL-10 and TGF-β) and hormonal dysregulation (insulin, leptin, adiponectin, FGF-21, testosterone and DHEA-S) in the serum of PCOS women (n=44), along with age matched controls (n=20), by ELISA. We quantified serum lipid peroxidation, protein peroxidation, and nitrite levels using spectrophotometry.

Results

PCOS women had significantly elevated levels of IL-27, IL-38 along with TNF-α, IL-6, IL-1Ra, IL-10, FGF-21 and adiponectin, and decreased levels of TGF-β, SDF-1 and leptin. While there is no significant difference with respect to redox markers, nitrite levels were significantly increased in PCOS cases.

Conclusion

The increased circulating levels of anti-inflammatory cytokines IL-27 and IL-38 under PCOS conditions warrant further investigation. To the best of our knowledge, this is the first report on IL-38 levels in PCOS.

背景多囊卵巢综合征（PCOS）是一种常见的代谢/内分泌紊乱疾病，主要见于育龄妇女，会增加不孕、子宫内膜癌和代谢紊乱的风险。IL-27和IL-38是最近发现的新型抗炎细胞因子，它们在多囊卵巢综合征免疫-内分泌功能失调中的作用尚不清楚。方法在本研究中，我们通过 ELISA 方法对多囊卵巢综合征妇女（44 人）和年龄匹配的对照组（20 人）血清中的这两种细胞因子以及元炎症标记物（TNF-α、IL-6、IL-1β、IL-1Ra、IL-10 和 TGF-β）和激素失调（胰岛素、瘦素、脂肪连素、FGF-21、睾酮和 DHEA-S）进行了量化。结果多囊卵巢综合征妇女的 IL-27、IL-38 以及 TNF-α、IL-6、IL-1Ra、IL-10、FGF-21 和脂肪连素水平显著升高，而 TGF-β、SDF-1 和瘦素水平下降。虽然在氧化还原标志物方面没有明显差异，但多囊卵巢综合征病例的亚硝酸盐水平明显升高。据我们所知，这是第一份关于多囊卵巢综合征中 IL-38 水平的报告。

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引用次数: 0

Oocyte donation pregnancies with high fetal-maternal immunogenetic dissimilarity show alterations in the maternal peripheral immunoregulatory response 胎儿与母体免疫遗传学高度相似的卵母细胞捐赠妊娠显示出母体外周免疫调节反应的改变

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-10-28 DOI: 10.1016/j.jri.2024.104387

K. van Bentem , L.J. Verleng , G.L. Lafeber , X. Tian , E. van Beelen , C. van der Keur , J.M. Kapsenberg , E.E.L.O. Lashley , M. Eikmans , M.L.P. van der Hoorn

Oocyte donation (OD) pregnancies result in increased fetal-maternal immunogenetic dissimilarity due to paternal and donor-derived genes. Higher fetal-maternal HLA mismatches are correlated with preeclampsia. Therefore, this study explored the maternal immune response, focusing on regulatory T cells (Tregs) during low versus high allogeneic pregnancies, and healthy versus preeclamptic OD pregnancies. Ten healthy and five preeclamptic OD pregnancies were included. Maternal peripheral blood was collected at different stages of pregnancy. Fetal-maternal HLA mismatches were determined, and immunophenotyping of peripheral blood mononuclear cells was conducted using a 22-colour spectral flow cytometry panel. Cytokines and hormones were detected in maternal plasma using ELISA and Luminex assays. The findings show similarities, but also distinct differences between low and high allogeneic healthy OD pregnancies. Early high allogeneic OD pregnancy showed reduction in Tregs, and CD8+ T cells, alongside lower percentage of effector/memory Tregs expressing PD-1 and Helios. Additionally, high allogeneic OD pregnancies showed increased IL-6 and progesterone in the first trimester. These variations suggest a different mode of immune regulation in early high allogeneic OD pregnancies, possibly to maintain healthy pregnancy. Further comparative analyses revealed reduced CD45RO+CTLA-4+ Tregs and increased latent TGF-β1 and -β2 levels in early preeclamptic compared to healthy OD pregnancy. Late-stage preeclamptic OD pregnancies exhibited higher frequencies of CD45RO+TIGIT+ Tregs and higher levels of TNFα, indicating both a regulatory and pro-inflammatory environment. Overall, this study sheds light on the course of various immunoregulatory key players in OD pregnancy, and expands knowledge on maternal tolerance in this particular type of pregnancy.

卵母细胞捐献（OD）妊娠会因父方和捐献者的基因而导致胎儿与母体的免疫遗传差异增加。胎儿与母体之间较高的 HLA 不匹配与子痫前期相关。因此，本研究探讨了母体的免疫反应，重点是低异体妊娠与高异体妊娠期间的调节性T细胞（Tregs），以及健康妊娠与子痫前期OD妊娠期间的调节性T细胞（Tregs）。研究对象包括 10 名健康孕妇和 5 名子痫前期 OD 孕妇。在妊娠的不同阶段采集母体外周血。采用 22 色光谱流式细胞仪对外周血单核细胞进行免疫分型。使用 ELISA 和 Luminex 检测法检测了母体血浆中的细胞因子和激素。研究结果表明，低异体健康外周血妊娠和高异体健康外周血妊娠既有相似之处，也有明显差异。早期高异体OD妊娠显示Tregs和CD8+ T细胞减少，同时表达PD-1和Helios的效应/记忆Tregs比例降低。此外，高异体OD妊娠在前三个月显示IL-6和孕酮增加。这些变化表明，早期高异体OD妊娠的免疫调节模式不同，可能是为了维持健康妊娠。进一步的比较分析表明，与健康外周妊娠相比，早期先兆子痫患者的 CD45RO+CTLA-4+ Tregs 减少，潜伏的 TGF-β1 和 -β2 水平升高。晚期子痫前期 OD 妊娠表现出更高频率的 CD45RO+TIGIT+ Tregs 和更高水平的 TNFα，表明存在调节性和促炎症环境。总之，这项研究揭示了OD妊娠中各种免疫调节关键因素的变化过程，并扩展了对这种特殊类型妊娠中母体耐受性的认识。

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引用次数: 0

The role and potential treatment of macrophages in patients with infertility and endometriosis 巨噬细胞在不孕症和子宫内膜异位症患者中的作用和潜在治疗方法。

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-10-19 DOI: 10.1016/j.jri.2024.104384

Linlin Song , Caihong Yang , Guiyi Ji , Rong Hu

Endometriosis is characterized as a macrophage-related ailment due to its strong link with immune dysfunction. Understanding the status of macrophage polarization in the context of endometriosis-related infertility is crucial for advancing diagnostic and therapeutic strategies. Our comprehensive review delves into the foundational understanding of macrophages and their profound influence on both endometriosis and infertility. Additionally, we illuminate the complex role of macrophages in infertility and endometriosis specifically. Finally, we focused on four critical dimensions: follicular fluid, the intraperitoneal environment, endometrial receptivity, and strategies for managing endometriosis. It is clear that throughout the progression of endometriosis, the diverse polarization states of macrophages play a pivotal role in the internal reproductive environment of infertile individuals grappling with this condition. Modulating macrophage polarization in the reproductive environment of endometriosis patients could address infertility challenges more effectively, offering a promising pathway for treating infertility associated with endometriosis.

子宫内膜异位症与免疫功能失调密切相关，因此被认为是一种与巨噬细胞有关的疾病。在子宫内膜异位症相关不孕症的背景下，了解巨噬细胞极化的状况对于推进诊断和治疗策略至关重要。我们的综合综述深入探讨了对巨噬细胞的基本认识及其对子宫内膜异位症和不孕症的深远影响。此外，我们还阐明了巨噬细胞在不孕症和子宫内膜异位症中的复杂作用。最后，我们重点讨论了四个关键方面：卵泡液、腹腔内环境、子宫内膜接受能力以及子宫内膜异位症的治疗策略。很明显，在子宫内膜异位症的整个发展过程中，巨噬细胞的不同极化状态在不孕患者的内部生殖环境中起着至关重要的作用。调节子宫内膜异位症患者生殖环境中巨噬细胞的极化状态可以更有效地解决不孕难题，为治疗与子宫内膜异位症相关的不孕症提供了一条前景广阔的途径。

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引用次数: 0

Reply to ‘‘The Association of Systemic Immune-Inflammation Index (SII), Systemic Immune-Response Index (SIRI), and Neutrophil-to-Lymphocyte Ratio (NLR) with Cesarean Scar Pregnancy (CSP)’’ 回复 "全身免疫炎症指数 (SII)、全身免疫反应指数 (SIRI) 和中性粒细胞与淋巴细胞比值 (NLR) 与剖宫产瘢痕妊娠 (CSP) 的关系"。

IF 2.9 3区医学 Q3 IMMUNOLOGY

Journal of Reproductive Immunology

Pub Date : 2024-10-18 DOI: 10.1016/j.jri.2024.104386

Refaettin Sahin, Atakan Tanacan, Dilek Sahin

We have read the correspondance written by Jin et al. with great interest. It is an honor for us to be followed by high quality academicians around the world. We want to thank the authors for their interest on our manuscript and in our opinion the manusript will be much more improved after our reply to the concerns underlined by Jin et al.All of the blood samples were collected before the administration of MTX in the present study. Cases with chronic diseases or infections that may affect the complete blood cell indices were excluded from the study. Thus, complete blood cell indices evaluated in the present study were not affected by the mentioned factors.We hope our reply will be sufficient to relieve the concerns of the readers. Thank you very much for your interest.

我们饶有兴趣地阅读了 Jin 等人的来信。我们很荣幸能受到世界各地高水平院士的关注。我们要感谢作者对我们稿件的关注，我们认为，在我们对 Jin 等人所强调的问题做出答复后，我们的稿件会有更大的改进。本研究排除了可能影响全血细胞指数的慢性疾病或感染病例。因此，本研究中评估的全血细胞指数不受上述因素的影响。非常感谢您的关注。

引用次数: 0