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Effect of iron oxid nanoparticle on the growth and physiology of inoculated alfalfa (Medicago sativa L.) with Rhizobium meliloti 纳米氧化铁对接种梅利洛根瘤菌苜蓿生长和生理的影响
Pub Date : 2020-03-20 DOI: 10.52547/jct.11.1.25
M. Askary, SM Talebi, M. Shafieigavari
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引用次数: 1
Antioxidant effect of carob seedextract (Ceratoniasiliqua L) on quality parameters Farahani ram sperm after freeze-thawing 角豆籽提取物(Ceratoniasiliqua L)对法拉哈尼公羊精子冻融后品质参数的抗氧化作用
Pub Date : 2020-03-20 DOI: 10.52547/jct.11.1.1
M. Asgari, M. Khodaei Motlagh, M. Kazemi- Bonchenari, V. Vahedi
{"title":"Antioxidant effect of carob seedextract (Ceratoniasiliqua L) on quality parameters Farahani ram sperm after freeze-thawing","authors":"M. Asgari, M. Khodaei Motlagh, M. Kazemi- Bonchenari, V. Vahedi","doi":"10.52547/jct.11.1.1","DOIUrl":"https://doi.org/10.52547/jct.11.1.1","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75520704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles 氧化锌纳米颗粒对乳腺癌细胞株(MCF-7)谷氨酸过氧化物酶和谷氨酸还原酶基因表达的影响
Pub Date : 2020-03-20 DOI: 10.52547/jct.11.1.44
M. Afshari, SA Sadat Shandiz, Smm HAmdi
{"title":"Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles","authors":"M. Afshari, SA Sadat Shandiz, Smm HAmdi","doi":"10.52547/jct.11.1.44","DOIUrl":"https://doi.org/10.52547/jct.11.1.44","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74677960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-veneris and apricot kernel extracts effects on the proliferation and apoptosis of breast cancer cells 水飞蓟、水飞蓟、紫锥菊、毛茛和杏仁提取物对乳腺癌细胞增殖和凋亡影响的比较
Pub Date : 2020-03-20 DOI: 10.52547/jct.11.1.73
L. Soltani, M. Darbemamieh
Aim: The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines ( Achillea wilhelmsii , Silybum marianum seed, Echinacea purpurea , Adiantum capillus-veneris and apricot kernel) against breast cancer cells (Mcf-7). Material and method: For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator.Different concentrations of herbal extracts (12.5, 25, 50 and 100 μ g/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange - ethidium bromide staining, respectively.Data was analyzed by SPSS software at the significant level of 5%. Results: Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (P<0.05) compared to other concentrations of the same extracts.Also, among the high concentrations (100μg/ml), the highest cell cytotoxicity effects were related to the extracts of Echinacea purpurea and Adiantum capillus-veneris (P<0.05). Conclusion: The results of this study indicated that the addition of Echinacea purpurea and Adiantum capillus-veneris extractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations.
目的:比较不同中草药(水仙、水飞蓟、紫锥菊、金针菇、杏仁)水醇提取物对乳腺癌细胞Mcf-7的增殖和凋亡作用。材料和方法:将植物干燥研磨,然后在70%乙醇中浸泡72小时,用旋转蒸发器提取其提取物。将12.5、25、50、100 μ g/ml不同浓度的草药提取物分别添加到癌细胞培养液中,24h后分别采用MTT法和吖啶橙-溴化乙啶染色法观察其细胞毒性和凋亡作用。数据采用SPSS软件分析,显著性水平为5%。结果:与其他浓度的提取物相比,在培养基中添加最高浓度的提取物对细胞增殖和凋亡的抑制作用最显著(P<0.05)。在高剂量浓度(100μg/ml)中,紫锥菊和毛茛提取物的细胞毒性作用最高(P<0.05)。结论:与其他植物提取物和浓度相比,紫锥菊和毛茛提取物高浓度对乳腺癌细胞的抗增殖和凋亡作用最显著。
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引用次数: 0
The effect of cadmium toxicity on health and risk index, coexistence and activity of some coriander antioxidant enzymes inoculated with Mycorrhiza fungi 镉中毒对接种菌根真菌后香菜几种抗氧化酶活性及健康风险指标的影响
Pub Date : 2020-03-20 DOI: 10.52547/jct.11.1.55
F. Mohammadifard, M. Moghaddam
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引用次数: 0
The Protective effect of Crocin on Ovary Mast cells, Blood Vessels and Ovary, Serum Biochemical changes following Busulfan-induced Oxidative Stress in Mice. 藏红花素对小鼠卵巢肥大细胞、血管和卵巢的保护作用及布苏丹诱导氧化应激后血清生化变化。
Pub Date : 2020-03-20 DOI: 10.52547/jct.11.1.13
H. Hassanzadeh Khanmiri, R. Shahrooz, S. Hassanzadeh, G. Najafi
Aim: The purpose of this research was aimed to evaluate the protective effects of crocin, as an antioxidant agent on Mast cells, blood vessels and biochemical changes of ovary and serum in Busulfan-induced oxidative stress in Mice. Material and Methods: Thirty mature 6-8 weeks aged female NMRI mice in the weight of 22-25 g were randomly divided into 6 groups, and treated for 21 days. The control group only received solvent of Busulfan (BSF) (0.1 ml) intraperitoneally, and BSF group received only Busulfan (10 mg kg -1 , IP/single dose). The experimental groups no. 1, 2, 3 received BSF (10 mg kg -1 /single dose) with crocin (100, 200, 400 mg kg -1 /day, IP) and positive group only received crocin (400 mg kg -1 , IP/day). At the end of treatment period, animals were euthanized and left ovary were studied for Mast cells, ovary blood vessels, and Serum, and right ovary for biochemical evaluations. Data was subjected to one‒way analysis of variance (ANOVA) and Tukey to determine if significant difference (P≤0.01) existed among the observed results using SPSS. Results: Busulfan significantly (P≤0.01) increased mast cells and MDA, while decreased ovary blood vessels and SOD rate, significantly (P=0.000) in comparison to control group. However, crocin in all the used doses, especially in the dose of 200 mg kg -1 , significantly decreased the adverse effects of Busulfan. Conclusion: The results indicated that crocin can protect ovaries against Busulfan induced damages, and it can be considered as a suitable drug for reducing the toxic effects of Busulfan in chemotherapy.
目的:探讨藏红花素作为抗氧化剂对布苏凡氧化应激小鼠肥大细胞、血管及卵巢和血清生化变化的保护作用。材料与方法:选取6-8周龄、体重22-25 g的成熟雌性NMRI小鼠30只,随机分为6组,每组治疗21 d。对照组仅给予布磺胺溶剂(BSF) (0.1 ml)腹腔注射,BSF组仅给予布磺胺(10 mg kg -1, IP/单次)。实验组无;1、2、3组分别给予BSF (10 mg kg -1 /单次剂量)和藏红花素(100、200、400 mg kg -1 /天,IP),阳性组仅给予藏红花素(400 mg kg -1, IP/天)。治疗期结束后,对大鼠左卵巢进行肥大细胞、卵巢血管和血清检测,对右卵巢进行生化评价。数据采用单因素方差分析(ANOVA)和双因素分析(Tukey),采用SPSS统计软件判断观察结果之间是否存在显著差异(P≤0.01)。结果:与对照组相比,布苏凡显著(P≤0.01)增加了肥大细胞和MDA,降低了卵巢血管和SOD的比例,差异有统计学意义(P=0.000)。然而,在所有使用剂量下,特别是在200 mg kg -1剂量下,藏红花素显著降低了布苏凡的不良反应。结论:藏红花素对布苏凡致卵巢损伤具有保护作用,可作为减轻布苏凡化疗毒副作用的合适药物。
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引用次数: 0
The Effect Of Immersion Time Variation in Polyvynyl Piprolidone Against Characteristics Of Scaffold Biocomposit Of Bacterial-Hydrocysiatatic Cellulose as Candidate 聚乙烯醇浸泡时间变化对细菌-水解纤维素候选支架生物复合材料特性的影响
Pub Date : 2019-02-23 DOI: 10.20473/JSCRTE.V2I2.11893
L. Indrio
Bone defects due to trauma, tumors, congenital abnormalities, degeneration and other diseases are still major problems in the field of orthopedics and traumatology. Based on data in Asia, Indonesia is the country with the highest number of fracture sufferers, there are as many as 300-400 cases of bone surgery per month in hospitals. Dr. Soetomo Surabaya (Gunawarman et al, 2010). Repair of damaged bones can be overcome with material that can accelerate the process of bone healing (bone healing). This research was conducted to synthesize hydroxyaparite bacterial cellulose scaffold as a candidate for bone healing. Bacterial cellulose as a matrix was synthesized by culturing Acetobacter xylnum, while hydroxyapatite as filler was synthesized by immersion into a solution of CaCl2 and Na2HPO4, the scaffold formation process using freeze dried method. Composite formation was varied by immersion in Polyvynil pirrolidone (PVP) for 0, 1, 2, 3, 4 days. Furthermore, samples were characterized using FTIR-Spectroscopy showing the presence of carbonates containing apatite crystals in all five samples.
创伤、肿瘤、先天性畸形、退行性变等疾病引起的骨缺损仍然是骨科和创伤学领域的主要问题。根据亚洲的数据,印度尼西亚是骨折患者人数最多的国家,医院每月有多达300-400例骨手术。Soetomo Surabaya博士(Gunawarman et al, 2010)。受损骨骼的修复可以用加速骨愈合过程的材料来克服。本研究旨在合成羟基磷灰石细菌纤维素支架作为骨愈合的候选材料。以细菌纤维素为基质,通过培养木醋杆菌合成,以羟基磷灰石为填料,在CaCl2和Na2HPO4溶液中浸泡合成,支架的形成过程采用冻干法。在聚乙烯吡咯烷酮(PVP)中浸泡0、1、2、3、4天后,复合物质的形成发生了变化。此外,使用红外光谱对样品进行了表征,显示在所有五个样品中都存在含有磷灰石晶体的碳酸盐。
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引用次数: 0
Potential Hollowfiber Polyurethane-Collagen of Chitosan Coatings As a Nerve Graft for the Therapy of Peripheral Nerve Injuries in Limb Paralysis 中空纤维聚氨酯-壳聚糖胶原膜神经移植治疗肢体瘫痪周围神经损伤的潜力
Pub Date : 2019-02-23 DOI: 10.20473/JSCRTE.V2I2.11892
H. Maulida
Peripheral nerve injury with a gap of 5–30 mm can cause permanent paralysis because it causes an axon to break up. The distance between axons of more than 1-2 cm requires a graft in the form of a nerve connector to fix it. Synthesis of chitosan coated polyurethane-collagen hollowfiber has been carried out as an accelerator for healing peripheral nerve injury. The results of Fourier Transform Infra Red (FTIR) analysis showed a cross link between chitosan and glutaraldehyde seen in the shift of wave numbers from 1080-1100 cm-1 to 1002 cm-1. The degradation test results showed that the sample experienced a decrease in mass after being immersed in Simulated Body Fluid (SBF) for 7 days. Polyurethane can be degraded in the body after 30 days. This is in accordance with the mechanism of the nerve which regenerates 1 mm per day or 1 inch per month. Scanning Electron Microscope (SEM) analysis showed that the diameter of the hollowfiber was 2.021-2.032 mm which corresponds to the peripheral nerve diameter of 1.5-3 mm and the pore size of the wall is 31.33-39.65 μm. The results of this study are expected to provide the theoretical basis for the use of chitosan polyurethane-collagen coating composites as nerve grafts for the treatment of peripheral nerve injuries that have biocompatible properties, can regenerate and are easily degraded and provide alternative solutions for nerve graft needs that are more economical and easier to manufacture so widely produced in Indonesia.
5 - 30mm间隙的周围神经损伤可引起永久性瘫痪,因为它会导致轴突断裂。轴突之间的距离超过1-2厘米,需要以神经连接的形式进行移植来固定。壳聚糖包被聚氨酯-胶原空心纤维的合成作为周围神经损伤愈合的促进剂。傅里叶变换红外(FTIR)分析结果表明,壳聚糖与戊二醛之间存在交联,波数从1080 ~ 1100 cm-1向1002 cm-1转变。降解试验结果表明,样品在模拟体液(SBF)中浸泡7天后,质量下降。30天后,聚氨酯可以在体内降解。这与神经每天再生1毫米或每月再生1英寸的机制是一致的。扫描电镜(SEM)分析显示,空心纤维直径为2.021 ~ 2.032 mm,对应周围神经直径1.5 ~ 3 mm,管壁孔径为31.33 ~ 39.65 μm。本研究结果有望为壳聚糖聚氨酯-胶原涂层复合材料作为神经移植物治疗周围神经损伤提供理论依据,该材料具有生物相容性、可再生、易降解等特点,并为印尼广泛生产的更经济、更容易制造的神经移植物需求提供替代解决方案。
{"title":"Potential Hollowfiber Polyurethane-Collagen of Chitosan Coatings As a Nerve Graft for the Therapy of Peripheral Nerve Injuries in Limb Paralysis","authors":"H. Maulida","doi":"10.20473/JSCRTE.V2I2.11892","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11892","url":null,"abstract":"Peripheral nerve injury with a gap of 5–30 mm can cause permanent paralysis because it causes an axon to break up. The distance between axons of more than 1-2 cm requires a graft in the form of a nerve connector to fix it. Synthesis of chitosan coated polyurethane-collagen hollowfiber has been carried out as an accelerator for healing peripheral nerve injury. The results of Fourier Transform Infra Red (FTIR) analysis showed a cross link between chitosan and glutaraldehyde seen in the shift of wave numbers from 1080-1100 cm-1 to 1002 cm-1. The degradation test results showed that the sample experienced a decrease in mass after being immersed in Simulated Body Fluid (SBF) for 7 days. Polyurethane can be degraded in the body after 30 days. This is in accordance with the mechanism of the nerve which regenerates 1 mm per day or 1 inch per month. Scanning Electron Microscope (SEM) analysis showed that the diameter of the hollowfiber was 2.021-2.032 mm which corresponds to the peripheral nerve diameter of 1.5-3 mm and the pore size of the wall is 31.33-39.65 μm. The results of this study are expected to provide the theoretical basis for the use of chitosan polyurethane-collagen coating composites as nerve grafts for the treatment of peripheral nerve injuries that have biocompatible properties, can regenerate and are easily degraded and provide alternative solutions for nerve graft needs that are more economical and easier to manufacture so widely produced in Indonesia.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86214597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cellullar Plasticity and Dedifferentiation: A Link Between Cancer Stem Cells, Hypoxia, Cell Injury, and Inflammation 细胞可塑性和去分化:癌症干细胞、缺氧、细胞损伤和炎症之间的联系
Pub Date : 2019-02-23 DOI: 10.20473/JSCRTE.V2I2.11655
A. Wijaya
Cellular plasticity is the concept of bidirectional dynamics change cells differentiation degree which involved in the regeneration, repair and tissue turnover along the organism livespan. Cellular plasticity and dedifferentiation process are well documented in the discovery of iPCSs by introducing several transcriptional factors known as Yamanaka factor to terminally differentiated somatic cells and reverted into pluripotent state as the ESCs. iPSCs are able to exhibit ESCs differentiation potential which could produce ectodermic, mesodermic, and endodermic cell lineage. In tumour biology, the tumour plasticity also have a similar regulation and play an imporant role for maintaining tumour integrity and survival, particularly in maintaining CSCs population. Various study of cellular plasticity regulation has shown that various factors are involved, in example hypoxia, cell injury, and inflammation. Cells respond to hypoxia, cell injury, and inflammation by chemoattractant which attract repair cells to homing towards injured sites. The homing mechanism of stem cells involved EMT to facilitates migration of stem cells towards injured sites, thus leading to tissue regeneration. On the other hand, cancer metastasis also showed a connection with EMT process. EMT which showed a change in cell properties are linked to dedifferentiation and hypoxia response. Hypoxia condition has been known to preserve and both normal stem cells and CSCs stemness. HIF which protected from degradation in hypoxia condition interact with DNA by binding to HRE. HRE activation trigger transcription of numerous signalling protein which involved in stemness, cell proliferation and survival. Therefore it is concluded that cell injury, hypoxia, and inflammation could programmed cells to undergo dedifferentiation process and involved in EMT regulations. CSCs which resides insides heterogeneous tumour cells population are though to be dynamicly regulate itself in the quietscent and active state through dedifferentiation like the normal stem cells. Understanding how CSCs regulates its active an quietscent state dynamics could provide an important information for novel CSCs targeted therapy development. 
细胞可塑性是细胞分化程度的双向动态变化的概念,它涉及生物体生命周期内的再生、修复和组织更新。在ipcs的发现过程中,细胞的可塑性和去分化过程被很好地记录下来,通过将几种转录因子(称为Yamanaka因子)引入到终末分化的体细胞中,并恢复到多能状态,即ESCs。iPSCs能够表现出内皮干细胞分化的潜力,可以产生外胚层、中胚层和内胚层细胞谱系。在肿瘤生物学中,肿瘤的可塑性也有类似的调控作用,对维持肿瘤的完整性和生存,特别是对维持CSCs的数量起着重要作用。对细胞可塑性调节的各种研究表明,多种因素参与其中,例如缺氧、细胞损伤和炎症。细胞对缺氧、细胞损伤和炎症的反应是通过化学引诱剂来吸引修复细胞回到受伤部位。干细胞的归巢机制涉及EMT促进干细胞向损伤部位迁移,从而导致组织再生。另一方面,肿瘤转移也与EMT过程有关。EMT显示细胞特性的改变与去分化和缺氧反应有关。缺氧条件可以保存正常干细胞和CSCs的干细胞性。HIF通过与HRE结合与DNA相互作用,在缺氧条件下防止降解。HRE的激活可触发多种信号蛋白的转录,这些信号蛋白参与细胞的生长、增殖和存活。因此,我们认为细胞损伤、缺氧和炎症可程序化细胞进行去分化过程,并参与EMT调控。存在于异质肿瘤细胞群中的CSCs与正常干细胞一样,通过去分化在安静和活跃状态下进行动态调节。了解CSCs如何调节其活跃和安静状态的动态,可以为新型CSCs靶向治疗的开发提供重要信息。
{"title":"Cellullar Plasticity and Dedifferentiation: A Link Between Cancer Stem Cells, Hypoxia, Cell Injury, and Inflammation","authors":"A. Wijaya","doi":"10.20473/JSCRTE.V2I2.11655","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11655","url":null,"abstract":"Cellular plasticity is the concept of bidirectional dynamics change cells differentiation degree which involved in the regeneration, repair and tissue turnover along the organism livespan. Cellular plasticity and dedifferentiation process are well documented in the discovery of iPCSs by introducing several transcriptional factors known as Yamanaka factor to terminally differentiated somatic cells and reverted into pluripotent state as the ESCs. iPSCs are able to exhibit ESCs differentiation potential which could produce ectodermic, mesodermic, and endodermic cell lineage. In tumour biology, the tumour plasticity also have a similar regulation and play an imporant role for maintaining tumour integrity and survival, particularly in maintaining CSCs population. Various study of cellular plasticity regulation has shown that various factors are involved, in example hypoxia, cell injury, and inflammation. Cells respond to hypoxia, cell injury, and inflammation by chemoattractant which attract repair cells to homing towards injured sites. The homing mechanism of stem cells involved EMT to facilitates migration of stem cells towards injured sites, thus leading to tissue regeneration. On the other hand, cancer metastasis also showed a connection with EMT process. EMT which showed a change in cell properties are linked to dedifferentiation and hypoxia response. Hypoxia condition has been known to preserve and both normal stem cells and CSCs stemness. HIF which protected from degradation in hypoxia condition interact with DNA by binding to HRE. HRE activation trigger transcription of numerous signalling protein which involved in stemness, cell proliferation and survival. Therefore it is concluded that cell injury, hypoxia, and inflammation could programmed cells to undergo dedifferentiation process and involved in EMT regulations. CSCs which resides insides heterogeneous tumour cells population are though to be dynamicly regulate itself in the quietscent and active state through dedifferentiation like the normal stem cells. Understanding how CSCs regulates its active an quietscent state dynamics could provide an important information for novel CSCs targeted therapy development. ","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86037327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stem Cell from Human Exfoliated Deciduous Teeth (SHED) versus Human Umbilical Cord Blood Mononuclear Cells (cbMNC) Transplantation in Neural Damage Reduction in Rat Model of Cerebral Ischemia 人脱落乳牙干细胞(SHED)与人脐血单个核细胞(cbMNC)移植对脑缺血大鼠神经损伤的影响
Pub Date : 2019-02-23 DOI: 10.20473/JSCRTE.V2I2.11896
Y. Ramli
Ischemic stroke is one of major cause of mortality and disability in Indonesia. Stem Cells are considered as a promising therapy for ischemic stroke. In this study, we compared therapeutic potency of Stem cell from human exfoliated deciduous teeth (SHED) and Human umbilical cord blood mononuclear cell (cbMNC) using rat models of ischemic stroke. Following middle cerebral artery occlusion (MCAO), twenty male wistar rats were divided into four groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as the control (stroke) group, rats undergone permanent MCAO and SHED transplantation (n=5) and rats undergone permanent MCAO and cbMNC transplantation (n=5) as the treatment group. SHED transplantation was performed at the acute phase after MCAO by intravenous injection. Histopathological evaluation of the neuron death ratio with hematoxylin and eosin staining confirmed that there was no significant differences at comparative study of neuron death ratio in rats transplanted with SHED and rats transplanted with cbMNC (p=0,81). SHED and cbMNC transplantation at acute stroke showed reduction in the neuron death ratio in the brain of rat models with ischemic stroke, and may provide an opportunity for neuroprotection and neural regeneration after ischemic stroke.
缺血性中风是印度尼西亚死亡和残疾的主要原因之一。干细胞被认为是一种很有前途的治疗缺血性中风的方法。在本研究中,我们比较了人脱落乳牙干细胞(SHED)和人脐带血单个核细胞(cbMNC)在缺血性脑卒中大鼠模型中的治疗作用。将20只雄性wistar大鼠分为正常大鼠(n=5)、脑中动脉闭塞(MCAO)大鼠(n=5)为对照(卒中)组、永久性MCAO + SHED移植大鼠(n=5)、永久性MCAO + cbMNC移植大鼠(n=5)为治疗组。在MCAO急性期静脉注射进行SHED移植。苏木精染色和伊红染色对神经元死亡比率进行组织病理学评价,证实SHED移植大鼠与cbMNC移植大鼠的神经元死亡比率比较研究无显著差异(p=0,81)。急性脑卒中移植SHED和cbMNC可降低缺血性脑卒中模型大鼠脑神经元死亡率,可能为缺血性脑卒中后的神经保护和神经再生提供机会。
{"title":"Stem Cell from Human Exfoliated Deciduous Teeth (SHED) versus Human Umbilical Cord Blood Mononuclear Cells (cbMNC) Transplantation in Neural Damage Reduction in Rat Model of Cerebral Ischemia","authors":"Y. Ramli","doi":"10.20473/JSCRTE.V2I2.11896","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11896","url":null,"abstract":"Ischemic stroke is one of major cause of mortality and disability in Indonesia. Stem Cells are considered as a promising therapy for ischemic stroke. In this study, we compared therapeutic potency of Stem cell from human exfoliated deciduous teeth (SHED) and Human umbilical cord blood mononuclear cell (cbMNC) using rat models of ischemic stroke. Following middle cerebral artery occlusion (MCAO), twenty male wistar rats were divided into four groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as the control (stroke) group, rats undergone permanent MCAO and SHED transplantation (n=5) and rats undergone permanent MCAO and cbMNC transplantation (n=5) as the treatment group. SHED transplantation was performed at the acute phase after MCAO by intravenous injection. Histopathological evaluation of the neuron death ratio with hematoxylin and eosin staining confirmed that there was no significant differences at comparative study of neuron death ratio in rats transplanted with SHED and rats transplanted with cbMNC (p=0,81). SHED and cbMNC transplantation at acute stroke showed reduction in the neuron death ratio in the brain of rat models with ischemic stroke, and may provide an opportunity for neuroprotection and neural regeneration after ischemic stroke.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89799161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of Stem Cell Research and Tissue Engineering
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