{"title":"Effect of iron oxid nanoparticle on the growth and physiology of inoculated alfalfa (Medicago sativa L.) with Rhizobium meliloti","authors":"M. Askary, SM Talebi, M. Shafieigavari","doi":"10.52547/jct.11.1.25","DOIUrl":"https://doi.org/10.52547/jct.11.1.25","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85936492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Asgari, M. Khodaei Motlagh, M. Kazemi- Bonchenari, V. Vahedi
{"title":"Antioxidant effect of carob seedextract (Ceratoniasiliqua L) on quality parameters Farahani ram sperm after freeze-thawing","authors":"M. Asgari, M. Khodaei Motlagh, M. Kazemi- Bonchenari, V. Vahedi","doi":"10.52547/jct.11.1.1","DOIUrl":"https://doi.org/10.52547/jct.11.1.1","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75520704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles","authors":"M. Afshari, SA Sadat Shandiz, Smm HAmdi","doi":"10.52547/jct.11.1.44","DOIUrl":"https://doi.org/10.52547/jct.11.1.44","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74677960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines ( Achillea wilhelmsii , Silybum marianum seed, Echinacea purpurea , Adiantum capillus-veneris and apricot kernel) against breast cancer cells (Mcf-7). Material and method: For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator.Different concentrations of herbal extracts (12.5, 25, 50 and 100 μ g/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange - ethidium bromide staining, respectively.Data was analyzed by SPSS software at the significant level of 5%. Results: Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (P<0.05) compared to other concentrations of the same extracts.Also, among the high concentrations (100μg/ml), the highest cell cytotoxicity effects were related to the extracts of Echinacea purpurea and Adiantum capillus-veneris (P<0.05). Conclusion: The results of this study indicated that the addition of Echinacea purpurea and Adiantum capillus-veneris extractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations.
{"title":"Comparison of Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-veneris and apricot kernel extracts effects on the proliferation and apoptosis of breast cancer cells","authors":"L. Soltani, M. Darbemamieh","doi":"10.52547/jct.11.1.73","DOIUrl":"https://doi.org/10.52547/jct.11.1.73","url":null,"abstract":"Aim: The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines ( Achillea wilhelmsii , Silybum marianum seed, Echinacea purpurea , Adiantum capillus-veneris and apricot kernel) against breast cancer cells (Mcf-7). Material and method: For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator.Different concentrations of herbal extracts (12.5, 25, 50 and 100 μ g/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange - ethidium bromide staining, respectively.Data was analyzed by SPSS software at the significant level of 5%. Results: Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (P<0.05) compared to other concentrations of the same extracts.Also, among the high concentrations (100μg/ml), the highest cell cytotoxicity effects were related to the extracts of Echinacea purpurea and Adiantum capillus-veneris (P<0.05). Conclusion: The results of this study indicated that the addition of Echinacea purpurea and Adiantum capillus-veneris extractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85518489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of cadmium toxicity on health and risk index, coexistence and activity of some coriander antioxidant enzymes inoculated with Mycorrhiza fungi","authors":"F. Mohammadifard, M. Moghaddam","doi":"10.52547/jct.11.1.55","DOIUrl":"https://doi.org/10.52547/jct.11.1.55","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73514007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Hassanzadeh Khanmiri, R. Shahrooz, S. Hassanzadeh, G. Najafi
Aim: The purpose of this research was aimed to evaluate the protective effects of crocin, as an antioxidant agent on Mast cells, blood vessels and biochemical changes of ovary and serum in Busulfan-induced oxidative stress in Mice. Material and Methods: Thirty mature 6-8 weeks aged female NMRI mice in the weight of 22-25 g were randomly divided into 6 groups, and treated for 21 days. The control group only received solvent of Busulfan (BSF) (0.1 ml) intraperitoneally, and BSF group received only Busulfan (10 mg kg -1 , IP/single dose). The experimental groups no. 1, 2, 3 received BSF (10 mg kg -1 /single dose) with crocin (100, 200, 400 mg kg -1 /day, IP) and positive group only received crocin (400 mg kg -1 , IP/day). At the end of treatment period, animals were euthanized and left ovary were studied for Mast cells, ovary blood vessels, and Serum, and right ovary for biochemical evaluations. Data was subjected to one‒way analysis of variance (ANOVA) and Tukey to determine if significant difference (P≤0.01) existed among the observed results using SPSS. Results: Busulfan significantly (P≤0.01) increased mast cells and MDA, while decreased ovary blood vessels and SOD rate, significantly (P=0.000) in comparison to control group. However, crocin in all the used doses, especially in the dose of 200 mg kg -1 , significantly decreased the adverse effects of Busulfan. Conclusion: The results indicated that crocin can protect ovaries against Busulfan induced damages, and it can be considered as a suitable drug for reducing the toxic effects of Busulfan in chemotherapy.
目的:探讨藏红花素作为抗氧化剂对布苏凡氧化应激小鼠肥大细胞、血管及卵巢和血清生化变化的保护作用。材料与方法:选取6-8周龄、体重22-25 g的成熟雌性NMRI小鼠30只，随机分为6组，每组治疗21 d。对照组仅给予布磺胺溶剂(BSF) (0.1 ml)腹腔注射，BSF组仅给予布磺胺(10 mg kg -1, IP/单次)。实验组无;1、2、3组分别给予BSF (10 mg kg -1 /单次剂量)和藏红花素(100、200、400 mg kg -1 /天，IP)，阳性组仅给予藏红花素(400 mg kg -1, IP/天)。治疗期结束后，对大鼠左卵巢进行肥大细胞、卵巢血管和血清检测，对右卵巢进行生化评价。数据采用单因素方差分析(ANOVA)和双因素分析(Tukey)，采用SPSS统计软件判断观察结果之间是否存在显著差异(P≤0.01)。结果:与对照组相比，布苏凡显著(P≤0.01)增加了肥大细胞和MDA，降低了卵巢血管和SOD的比例，差异有统计学意义(P=0.000)。然而，在所有使用剂量下，特别是在200 mg kg -1剂量下，藏红花素显著降低了布苏凡的不良反应。结论:藏红花素对布苏凡致卵巢损伤具有保护作用，可作为减轻布苏凡化疗毒副作用的合适药物。
{"title":"The Protective effect of Crocin on Ovary Mast cells, Blood Vessels and Ovary, Serum Biochemical changes following Busulfan-induced Oxidative Stress in Mice.","authors":"H. Hassanzadeh Khanmiri, R. Shahrooz, S. Hassanzadeh, G. Najafi","doi":"10.52547/jct.11.1.13","DOIUrl":"https://doi.org/10.52547/jct.11.1.13","url":null,"abstract":"Aim: The purpose of this research was aimed to evaluate the protective effects of crocin, as an antioxidant agent on Mast cells, blood vessels and biochemical changes of ovary and serum in Busulfan-induced oxidative stress in Mice. Material and Methods: Thirty mature 6-8 weeks aged female NMRI mice in the weight of 22-25 g were randomly divided into 6 groups, and treated for 21 days. The control group only received solvent of Busulfan (BSF) (0.1 ml) intraperitoneally, and BSF group received only Busulfan (10 mg kg -1 , IP/single dose). The experimental groups no. 1, 2, 3 received BSF (10 mg kg -1 /single dose) with crocin (100, 200, 400 mg kg -1 /day, IP) and positive group only received crocin (400 mg kg -1 , IP/day). At the end of treatment period, animals were euthanized and left ovary were studied for Mast cells, ovary blood vessels, and Serum, and right ovary for biochemical evaluations. Data was subjected to one‒way analysis of variance (ANOVA) and Tukey to determine if significant difference (P≤0.01) existed among the observed results using SPSS. Results: Busulfan significantly (P≤0.01) increased mast cells and MDA, while decreased ovary blood vessels and SOD rate, significantly (P=0.000) in comparison to control group. However, crocin in all the used doses, especially in the dose of 200 mg kg -1 , significantly decreased the adverse effects of Busulfan. Conclusion: The results indicated that crocin can protect ovaries against Busulfan induced damages, and it can be considered as a suitable drug for reducing the toxic effects of Busulfan in chemotherapy.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75821162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-23DOI: 10.20473/JSCRTE.V2I2.11893
L. Indrio
Bone defects due to trauma, tumors, congenital abnormalities, degeneration and other diseases are still major problems in the field of orthopedics and traumatology. Based on data in Asia, Indonesia is the country with the highest number of fracture sufferers, there are as many as 300-400 cases of bone surgery per month in hospitals. Dr. Soetomo Surabaya (Gunawarman et al, 2010). Repair of damaged bones can be overcome with material that can accelerate the process of bone healing (bone healing). This research was conducted to synthesize hydroxyaparite bacterial cellulose scaffold as a candidate for bone healing. Bacterial cellulose as a matrix was synthesized by culturing Acetobacter xylnum, while hydroxyapatite as filler was synthesized by immersion into a solution of CaCl2 and Na2HPO4, the scaffold formation process using freeze dried method. Composite formation was varied by immersion in Polyvynil pirrolidone (PVP) for 0, 1, 2, 3, 4 days. Furthermore, samples were characterized using FTIR-Spectroscopy showing the presence of carbonates containing apatite crystals in all five samples.
创伤、肿瘤、先天性畸形、退行性变等疾病引起的骨缺损仍然是骨科和创伤学领域的主要问题。根据亚洲的数据，印度尼西亚是骨折患者人数最多的国家，医院每月有多达300-400例骨手术。Soetomo Surabaya博士(Gunawarman et al, 2010)。受损骨骼的修复可以用加速骨愈合过程的材料来克服。本研究旨在合成羟基磷灰石细菌纤维素支架作为骨愈合的候选材料。以细菌纤维素为基质，通过培养木醋杆菌合成，以羟基磷灰石为填料，在CaCl2和Na2HPO4溶液中浸泡合成，支架的形成过程采用冻干法。在聚乙烯吡咯烷酮(PVP)中浸泡0、1、2、3、4天后，复合物质的形成发生了变化。此外，使用红外光谱对样品进行了表征，显示在所有五个样品中都存在含有磷灰石晶体的碳酸盐。
{"title":"The Effect Of Immersion Time Variation in Polyvynyl Piprolidone Against Characteristics Of Scaffold Biocomposit Of Bacterial-Hydrocysiatatic Cellulose as Candidate","authors":"L. Indrio","doi":"10.20473/JSCRTE.V2I2.11893","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11893","url":null,"abstract":"Bone defects due to trauma, tumors, congenital abnormalities, degeneration and other diseases are still major problems in the field of orthopedics and traumatology. Based on data in Asia, Indonesia is the country with the highest number of fracture sufferers, there are as many as 300-400 cases of bone surgery per month in hospitals. Dr. Soetomo Surabaya (Gunawarman et al, 2010). Repair of damaged bones can be overcome with material that can accelerate the process of bone healing (bone healing). This research was conducted to synthesize hydroxyaparite bacterial cellulose scaffold as a candidate for bone healing. Bacterial cellulose as a matrix was synthesized by culturing Acetobacter xylnum, while hydroxyapatite as filler was synthesized by immersion into a solution of CaCl2 and Na2HPO4, the scaffold formation process using freeze dried method. Composite formation was varied by immersion in Polyvynil pirrolidone (PVP) for 0, 1, 2, 3, 4 days. Furthermore, samples were characterized using FTIR-Spectroscopy showing the presence of carbonates containing apatite crystals in all five samples.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79315267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-23DOI: 10.20473/JSCRTE.V2I2.11892
H. Maulida
Peripheral nerve injury with a gap of 5–30 mm can cause permanent paralysis because it causes an axon to break up. The distance between axons of more than 1-2 cm requires a graft in the form of a nerve connector to fix it. Synthesis of chitosan coated polyurethane-collagen hollowfiber has been carried out as an accelerator for healing peripheral nerve injury. The results of Fourier Transform Infra Red (FTIR) analysis showed a cross link between chitosan and glutaraldehyde seen in the shift of wave numbers from 1080-1100 cm-1 to 1002 cm-1. The degradation test results showed that the sample experienced a decrease in mass after being immersed in Simulated Body Fluid (SBF) for 7 days. Polyurethane can be degraded in the body after 30 days. This is in accordance with the mechanism of the nerve which regenerates 1 mm per day or 1 inch per month. Scanning Electron Microscope (SEM) analysis showed that the diameter of the hollowfiber was 2.021-2.032 mm which corresponds to the peripheral nerve diameter of 1.5-3 mm and the pore size of the wall is 31.33-39.65 μm. The results of this study are expected to provide the theoretical basis for the use of chitosan polyurethane-collagen coating composites as nerve grafts for the treatment of peripheral nerve injuries that have biocompatible properties, can regenerate and are easily degraded and provide alternative solutions for nerve graft needs that are more economical and easier to manufacture so widely produced in Indonesia.
{"title":"Potential Hollowfiber Polyurethane-Collagen of Chitosan Coatings As a Nerve Graft for the Therapy of Peripheral Nerve Injuries in Limb Paralysis","authors":"H. Maulida","doi":"10.20473/JSCRTE.V2I2.11892","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11892","url":null,"abstract":"Peripheral nerve injury with a gap of 5–30 mm can cause permanent paralysis because it causes an axon to break up. The distance between axons of more than 1-2 cm requires a graft in the form of a nerve connector to fix it. Synthesis of chitosan coated polyurethane-collagen hollowfiber has been carried out as an accelerator for healing peripheral nerve injury. The results of Fourier Transform Infra Red (FTIR) analysis showed a cross link between chitosan and glutaraldehyde seen in the shift of wave numbers from 1080-1100 cm-1 to 1002 cm-1. The degradation test results showed that the sample experienced a decrease in mass after being immersed in Simulated Body Fluid (SBF) for 7 days. Polyurethane can be degraded in the body after 30 days. This is in accordance with the mechanism of the nerve which regenerates 1 mm per day or 1 inch per month. Scanning Electron Microscope (SEM) analysis showed that the diameter of the hollowfiber was 2.021-2.032 mm which corresponds to the peripheral nerve diameter of 1.5-3 mm and the pore size of the wall is 31.33-39.65 μm. The results of this study are expected to provide the theoretical basis for the use of chitosan polyurethane-collagen coating composites as nerve grafts for the treatment of peripheral nerve injuries that have biocompatible properties, can regenerate and are easily degraded and provide alternative solutions for nerve graft needs that are more economical and easier to manufacture so widely produced in Indonesia.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86214597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-23DOI: 10.20473/JSCRTE.V2I2.11655
A. Wijaya
Cellular plasticity is the concept of bidirectional dynamics change cells differentiation degree which involved in the regeneration, repair and tissue turnover along the organism livespan. Cellular plasticity and dedifferentiation process are well documented in the discovery of iPCSs by introducing several transcriptional factors known as Yamanaka factor to terminally differentiated somatic cells and reverted into pluripotent state as the ESCs. iPSCs are able to exhibit ESCs differentiation potential which could produce ectodermic, mesodermic, and endodermic cell lineage. In tumour biology, the tumour plasticity also have a similar regulation and play an imporant role for maintaining tumour integrity and survival, particularly in maintaining CSCs population. Various study of cellular plasticity regulation has shown that various factors are involved, in example hypoxia, cell injury, and inflammation. Cells respond to hypoxia, cell injury, and inflammation by chemoattractant which attract repair cells to homing towards injured sites. The homing mechanism of stem cells involved EMT to facilitates migration of stem cells towards injured sites, thus leading to tissue regeneration. On the other hand, cancer metastasis also showed a connection with EMT process. EMT which showed a change in cell properties are linked to dedifferentiation and hypoxia response. Hypoxia condition has been known to preserve and both normal stem cells and CSCs stemness. HIF which protected from degradation in hypoxia condition interact with DNA by binding to HRE. HRE activation trigger transcription of numerous signalling protein which involved in stemness, cell proliferation and survival. Therefore it is concluded that cell injury, hypoxia, and inflammation could programmed cells to undergo dedifferentiation process and involved in EMT regulations. CSCs which resides insides heterogeneous tumour cells population are though to be dynamicly regulate itself in the quietscent and active state through dedifferentiation like the normal stem cells. Understanding how CSCs regulates its active an quietscent state dynamics could provide an important information for novel CSCs targeted therapy development.
{"title":"Cellullar Plasticity and Dedifferentiation: A Link Between Cancer Stem Cells, Hypoxia, Cell Injury, and Inflammation","authors":"A. Wijaya","doi":"10.20473/JSCRTE.V2I2.11655","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11655","url":null,"abstract":"Cellular plasticity is the concept of bidirectional dynamics change cells differentiation degree which involved in the regeneration, repair and tissue turnover along the organism livespan. Cellular plasticity and dedifferentiation process are well documented in the discovery of iPCSs by introducing several transcriptional factors known as Yamanaka factor to terminally differentiated somatic cells and reverted into pluripotent state as the ESCs. iPSCs are able to exhibit ESCs differentiation potential which could produce ectodermic, mesodermic, and endodermic cell lineage. In tumour biology, the tumour plasticity also have a similar regulation and play an imporant role for maintaining tumour integrity and survival, particularly in maintaining CSCs population. Various study of cellular plasticity regulation has shown that various factors are involved, in example hypoxia, cell injury, and inflammation. Cells respond to hypoxia, cell injury, and inflammation by chemoattractant which attract repair cells to homing towards injured sites. The homing mechanism of stem cells involved EMT to facilitates migration of stem cells towards injured sites, thus leading to tissue regeneration. On the other hand, cancer metastasis also showed a connection with EMT process. EMT which showed a change in cell properties are linked to dedifferentiation and hypoxia response. Hypoxia condition has been known to preserve and both normal stem cells and CSCs stemness. HIF which protected from degradation in hypoxia condition interact with DNA by binding to HRE. HRE activation trigger transcription of numerous signalling protein which involved in stemness, cell proliferation and survival. Therefore it is concluded that cell injury, hypoxia, and inflammation could programmed cells to undergo dedifferentiation process and involved in EMT regulations. CSCs which resides insides heterogeneous tumour cells population are though to be dynamicly regulate itself in the quietscent and active state through dedifferentiation like the normal stem cells. Understanding how CSCs regulates its active an quietscent state dynamics could provide an important information for novel CSCs targeted therapy development. ","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86037327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-23DOI: 10.20473/JSCRTE.V2I2.11896
Y. Ramli
Ischemic stroke is one of major cause of mortality and disability in Indonesia. Stem Cells are considered as a promising therapy for ischemic stroke. In this study, we compared therapeutic potency of Stem cell from human exfoliated deciduous teeth (SHED) and Human umbilical cord blood mononuclear cell (cbMNC) using rat models of ischemic stroke. Following middle cerebral artery occlusion (MCAO), twenty male wistar rats were divided into four groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as the control (stroke) group, rats undergone permanent MCAO and SHED transplantation (n=5) and rats undergone permanent MCAO and cbMNC transplantation (n=5) as the treatment group. SHED transplantation was performed at the acute phase after MCAO by intravenous injection. Histopathological evaluation of the neuron death ratio with hematoxylin and eosin staining confirmed that there was no significant differences at comparative study of neuron death ratio in rats transplanted with SHED and rats transplanted with cbMNC (p=0,81). SHED and cbMNC transplantation at acute stroke showed reduction in the neuron death ratio in the brain of rat models with ischemic stroke, and may provide an opportunity for neuroprotection and neural regeneration after ischemic stroke.
{"title":"Stem Cell from Human Exfoliated Deciduous Teeth (SHED) versus Human Umbilical Cord Blood Mononuclear Cells (cbMNC) Transplantation in Neural Damage Reduction in Rat Model of Cerebral Ischemia","authors":"Y. Ramli","doi":"10.20473/JSCRTE.V2I2.11896","DOIUrl":"https://doi.org/10.20473/JSCRTE.V2I2.11896","url":null,"abstract":"Ischemic stroke is one of major cause of mortality and disability in Indonesia. Stem Cells are considered as a promising therapy for ischemic stroke. In this study, we compared therapeutic potency of Stem cell from human exfoliated deciduous teeth (SHED) and Human umbilical cord blood mononuclear cell (cbMNC) using rat models of ischemic stroke. Following middle cerebral artery occlusion (MCAO), twenty male wistar rats were divided into four groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as the control (stroke) group, rats undergone permanent MCAO and SHED transplantation (n=5) and rats undergone permanent MCAO and cbMNC transplantation (n=5) as the treatment group. SHED transplantation was performed at the acute phase after MCAO by intravenous injection. Histopathological evaluation of the neuron death ratio with hematoxylin and eosin staining confirmed that there was no significant differences at comparative study of neuron death ratio in rats transplanted with SHED and rats transplanted with cbMNC (p=0,81). SHED and cbMNC transplantation at acute stroke showed reduction in the neuron death ratio in the brain of rat models with ischemic stroke, and may provide an opportunity for neuroprotection and neural regeneration after ischemic stroke.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89799161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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