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D-グルコサミンからD-グルコサミン酸への微生物変換 从D-葡萄糖胺到D-葡萄糖胺酸的微生物转换
泰之 滝口, 椎名 亜弥子, 達明 山口
Strain A-10, which oxidizes D-glucosamine (D-GlcN) to D-glucosaminic acid (D-GlcNA), was isolated from a chitincontaining soil in Chiba, Japan. The strain was identified as Acinetobacter sp. by the 16S rRNA homology and some physiological tests. D-GlcN in the liquid medium disappeared within 48h, and D-GlcNA was accumulated in the medium. It is remarkable that the maximum yield of GlcNA reached 92 mol% based on D-GlcN. The FT-IR specrum of the isolated D-GlcNA was in fair agreement with the commercial D-GlcNA.
从日本千叶含几丁质土壤中分离到一株能将d -氨基葡萄糖(D-GlcN)氧化为d -氨基葡萄糖酸(D-GlcNA)的菌株a -10。经16S rRNA同源性和一些生理试验鉴定该菌株为不动杆菌。液体培养基中的D-GlcN在48h内消失,D-GlcNA在培养基中积累。值得注意的是,在D-GlcN的基础上,glcn的产率最高可达92 mol%。分离得到的D-GlcNA的FT-IR光谱与市售的D-GlcNA基本一致。
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引用次数: 6
Use of mannooligosaccharides from coffee mannan by intestinal bacteria 利用肠道细菌从咖啡甘露聚糖中提取甘露寡糖
I. Asano, Yasuyuki Nakamura, H. Hoshino, Keiji Aoki, Shigeyoshi Fujii, Naoto Imura, H. Iino
コーヒー抽出粕の熱加水分解抽出物を脱塩,脱色して精製したマンノオリゴ糖混合物から,活性炭クロマトグラフィーによりβ-1, 4-D-マンノビオース, β-1, 4-D-マンノトリオース, β-1, 4-D-マンノテトラオース, β-1, 4-D-マンノペンタオースを単離精製した.これらのマンノオリゴ糖について,ヒト腸内フローラ構成菌種による資化性を調べた.重合度にかかわらずマンノオリゴ糖は, Bifidobacterium adolescentisとLactobacillus acidophilus, Lactobacillus gasseriに利用された.一方腸内有害菌にはほとんど利用されなかった.その結果,マンノオリゴ糖にはヒト腸内フローラの改善作用をもっことが期待できると考えられた.
将咖啡提取糟的热水解萃取物脱盐、脱色精制而成的甘露低聚糖混合物,通过活性炭色度法制成β- 1,4 -D-甘露聚糖,β- 1,4 -D-甘露三聚糖,β-1,分离纯化了4-D- man的四聚糖,β- 1,4 -D- man的戊聚糖。对于这些甘露的低聚糖,研究了人类肠道菌群构成菌种的资化性。无论重聚度如何,曼诺低聚糖分别为Bifidobacterium adolescentis和Lactobacillus acidophilus;被Lactobacillus gasseri所利用。另一方面,肠内有害菌几乎没有利用。结果认为,曼诺低聚糖具有改善人类肠内菌群的作用。
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引用次数: 34
ガスクロマトグラフ-燃焼-同位体比質量分析計によるホウレンソウ中のシュウ酸の炭素同位体組成分析法 通过气相色谱-燃烧-同位素比质谱仪分析菠菜中草酸的碳同位素组成
F. Tanaka, Kim Tae-Hwan, T. Yoneyama
An analytical method to measure 13C abundance in 13C enriched oxalate using gas chromatograph/combustion/isotope ratio mass spectormeter (GC/C/IRMS) was established. The 13C atom% values of oxalate measured by GC/C/IRMS were nearly equal to those measured by automated nitrogen and carbon analyzer-mass spectrometer (ANCA-MS) giving a high degree of correlation (r2=0.9999) and good reproducibility (CV=0.224%). This method will be applicable in the study of oxalate metabolism in spinach.
建立了气相色谱/燃烧/同位素比质谱仪(GC/C/IRMS)测定富13C草酸盐中13C丰度的分析方法。GC/C/IRMS测定的草酸13C原子%值与全自动氮碳分析仪-质谱联用仪(ANCA-MS)测定值基本相等,相关性高(r2=0.9999),重现性好(CV=0.224%)。该方法可用于菠菜草酸代谢的研究。
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引用次数: 1
Characteristics of a Simple Assay Method for .ALPHA.-Amylase Using Microplates. α - α简易测定方法的特点。-淀粉酶使用微孔板。
Toshiya Satoyama, Yoshie Nakai, Y. Hirata, K. Tanno, T. Hara, Y. Fujio
The characteristics of a novel method of α-amylase activity determination were investigated. The principles of this method were based on the light absorbance change through a gelatinized starch-agar gel fixed in a well of a microplate caused by α-amylase action. α-Amylases from different origins were successfully measured. The results of α-amylase activity in enzyme formulations and rice koji extractions measured by this method were in agreement with the values obtained by the conventional method. Since the microplate method is only able to measure α-amylase activity selectively, this method is considered to be extremely practical for quick, multisample treatment.
研究了α-淀粉酶活性测定新方法的特点。该方法的原理是基于α-淀粉酶作用引起的α-淀粉酶在微孔板中固定淀粉-琼脂凝胶的光吸光度变化。成功地测定了不同来源的α-淀粉酶。该方法测定的酶制剂和米曲提取物α-淀粉酶活性与常规方法测定的结果基本一致。由于微孔板法只能选择性地测量α-淀粉酶活性,因此该方法被认为是非常实用的快速多样品处理方法。
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引用次数: 2
CONTROL OF CELL MORPHOGENESIS AND THE CELL CYCLE IN FISSION YEAST 裂变酵母细胞形态发生和细胞周期的控制
David J. Wiley, F. Verde
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引用次数: 0
A Simple Assay Method for .ALPHA.-Amylase Using Microplates. 一种测定α的简便方法。-淀粉酶使用微孔板。
Toshiya Satoyama, T. Hara, M. Murata, Y. Fujio
A novel method for measuring α-amylase-activity was developed in this study. By adding an authentic α-amylase solution to a mixture of agar and gelatinized rice starch, which was fixed in a well of a microplate, the gel turbidity measured at 655 nm was decreased by the α-amylase action on the gelatinized starch. The intensity of the α-amylase-activity was in proportion logarithmically to the turbidity decrease detected by a microplate-reader. From reasonable values of standard deviation and coefficient of variation (3.7_??_8.9%) of the obtained data, this method makes it possible to measure α-amylase-activity conveniently.
本研究提出了一种新的α-淀粉酶活性测定方法。将α-淀粉酶原液加入琼脂和糯米淀粉的混合物中,固定在微孔板中,α-淀粉酶作用于糯米淀粉,降低了655 nm处的凝胶浊度。α-淀粉酶活性的强度与微孔板阅读器检测到的浊度降低成对数比例。所得数据的标准差和变异系数(3.7 ̄8.9%)合理,为α-淀粉酶活性的测定提供了方便。
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引用次数: 5
Regulation of gene expression by vitamin D 维生素D对基因表达的调控
T. Yoshizawa, K. Sekine, S. Kato
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引用次数: 3
Anti-inflammatory effect of oolong tea polyphenols 乌龙茶多酚的抗炎作用
K. Nakazato, T. Takeo
The anti-inflammatory activity of aqueous oolong tea extract was tested. The formation of edema caused in rats paws by injection of carrageenin was effectively depressed by soaking the rat paws in hot oolong tea extract. It gives 60% inhibition at the dose of 75 ppm of oolong tea extract. Oolong tea extract showed higher anti-inflammatory activity than those of green and black tea extracts. The active components were found to be catechins and oolong tea tannin (oxidative products of catechin in oolong tea) fractionated from aqueous oolong tea extract. By oral administration of oolong tea extract, carrageenin-induced inflammation was also inhibited, but the anti-inflammatory avtivity by oral administration was lower than by the soaking treatment. The inflammatory activity of percutaneous absorbed oolong tea polyphenols was assumed to be due, at least partly, to the inhibition of prostaglandin biosynthesis in rat tissue injected with carrageenin.
研究了乌龙茶水提物的抗炎活性。用热乌龙茶浸膏浸泡大鼠足部,可有效抑制角叉菜胶致大鼠足部水肿的形成。当乌龙茶提取物浓度为75ppm时,抑制率为60%。乌龙茶提取物的抗炎活性高于绿茶和红茶提取物。从乌龙茶水提取物中分离出有效成分为儿茶素和乌龙茶单宁(乌龙茶中儿茶素的氧化产物)。乌龙茶提取物对角叉菜胶诱导的炎症也有抑制作用,但其抗炎活性低于浸泡处理。经皮吸收乌龙茶多酚的炎症活性被认为是由于,至少部分原因是,在大鼠组织中注射了卡拉胶抑制前列腺素的生物合成。
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引用次数: 8
Effects of Dietary Sake Cake on Cholesterol Metabolism in Rats 清饼对大鼠胆固醇代谢的影响
Yuko Ashida, Yoshiyuki Saito, Akitsugu Kawato, K. Suginami, S. Imayasu
Sake cake prepared by the new sake brewing method using liquefied rice is far richer in protein than the one from the former method. Its nutritive value and hypocholesterolemic effect were examined. Growing rats were fed for 3 and 4 weeks on 33.3% sake cake diets with and without 0.5% cholesterol (0.15% sodium cholate). There was no significant difference in growth between the sake cake and casein groups. The serum and liver cholesterol levels in the sake cake group were significantly lower than those in the casein group. Sake cake feeding increased fecal excretion of bile salts, cholesterol, and lipid more as compared with casein feeding. A similar or greater effect was observed for the group given the pepsin undigested fraction of sake cake. (Received June 5, 1996) Key words; sake cake; rice protein; cholesterol; protein nutrition; hypocholesterolemic effect
用液化大米酿造的清酒饼,其蛋白质含量远高于原方法酿造的清酒饼。考察其营养价值和降胆固醇作用。饲养3周和4周,分别饲喂33.3%清饼和不添加0.5%胆固醇(0.15%胆酸钠)的日粮。清酒饼组和酪蛋白组之间的生长无显著差异。清酒饼组的血清和肝脏胆固醇水平显著低于酪蛋白组。与饲喂酪蛋白相比,饲喂清酒饼增加了粪便中胆盐、胆固醇和脂类的排泄。对于服用清酒蛋糕中未消化部分的胃蛋白酶的一组,观察到类似或更大的效果。(收于1996年6月5日)关键词;为了蛋糕;大米蛋白;胆固醇;蛋白质营养;hypocholesterolemic效应
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引用次数: 11
Size effects on microorganisms destroyed by shock waves 被冲击波破坏的微生物的大小效应
K. Fujiwara, M. Asakawa, H. Matsuo, T. Hiroe, S. Hori
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Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry
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