{"title":"黒毛和種牛枝肉表面の綿球法及びスポンジ法による衛生指標菌数の比較","authors":"Mitsushi KOBAYASHI, Kouji FURUUCHI, Jin ONODERA, Fumiaki KOIKE, Yoshihiro TSUJI, Mayumi SHIMAMURA, Hajime TOYOFUKU","doi":"10.12935/jvma.76.e270","DOIUrl":"https://doi.org/10.12935/jvma.76.e270","url":null,"abstract":"牛枝肉表面の綿球法及びスポンジ法による衛生指標菌数を比較した．2021年3月から一年間，検体数各100件，綿球法は枝肉のもも，ばら（上部），ばら（下部），かたばら（各100cm2）の4カ所（計400cm2），スポンジ法は綿球法の隣接部位を計400cm2拭き取った．検体の綿球法による一般生菌数及び腸内細菌科菌群数の平均±標準偏差（log cfu/cm2）は各々2.04±0.66，0.47±0.28，スポンジ法は各々3.15±0.40及び0.77±0.62であり，スポンジ法の一般生菌数及び腸内細菌科菌群数は綿球法のそれらと比べて高かった（P＜0.001）．スポンジ法では検知できる警戒レベルの一般生菌数及び腸内細菌科菌群数を綿球法では検出されないことが多いため，今後はスポンジ法によるサンプリングを行うべきであると考えられた．","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135843478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Orthovoltage Radiation Therapy of Oral Malignant Melanomas in 17 Dogs","authors":"M. Tagawa, Genya Shimbo, M. Tomihari, K. Miyahara","doi":"10.12935/jvma.76.e55","DOIUrl":"https://doi.org/10.12935/jvma.76.e55","url":null,"abstract":"","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87230121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"黒毛和種育成雌牛でみられた多発性の皮膚粘液腫の1例","authors":"Riko ITO, Hiroshi KANNO, Keinosuke SANADA, Kazuhiro OTA","doi":"10.12935/jvma.76.e313","DOIUrl":"https://doi.org/10.12935/jvma.76.e313","url":null,"abstract":"11カ月齢の黒毛和種雌牛において胸垂に直径6 cmの有茎状の腫瘤が発生し，腫瘤の切除を行った．病理組織学的及び免疫組織学的所見に基づき粘液腫と診断した．術後約2カ月で切除部位に腫瘍が再発し，その後1カ月間に右肩部，左右頸部及び下顎に0.5～1 cmの小型の同腫瘍が多発したため，計5カ所の切除を行った．臨床経過，病理組織学的所見から，多発性粘液腫と診断した．術後10カ月時点では切除部位での再発は認められず，新たな腫瘍も発生していない．牛において粘液腫はまれな腫瘍であり，外科的治療に関する報告は少なく，これまで多発した症例の報告はない．皮膚粘液腫が多発した要因について，本症例では精査できていないが，人においては遺伝子異常に起因する疾患があるため，今後も腫瘍の発生について経過観察が必要と考えられる．","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135611104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kohtaro Miyazawa, Y. Matsuura, Keigo Ikeda, Y. Iwamaru
{"title":"Nanopore Sequencing as a Rapid Tool for Discrimination between Epidemic and Vaccine Strains of Classical Swine Fever Viruses in Japan","authors":"Kohtaro Miyazawa, Y. Matsuura, Keigo Ikeda, Y. Iwamaru","doi":"10.12935/jvma.75.e83","DOIUrl":"https://doi.org/10.12935/jvma.75.e83","url":null,"abstract":"","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73900315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Testicular Vascular Hamartoma in a Calf","authors":"Susumu Tosa, M. Yamada","doi":"10.12935/jvma.75.e9","DOIUrl":"https://doi.org/10.12935/jvma.75.e9","url":null,"abstract":"","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75603182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fumiko Suzuta, Yoshimi Terayama, Ryou Urakawa, Asuka Kumagai, K. Kimura
A 4-day-old Japanese Black calf presented with fever as well as respiratory and neurological symptoms and died at the age of 17 days. Necropsy revealed multiple white nodules in the peritoneum and liver, erosions and ulcers in the forestomach and abomasum, and cerebrospinal fluid opacity. Microbiological examination was used to isolate Candida albicans from multiple organs, including the brain. The causative agent of the lesions was confirmed to be C. albicans using immunohistochemistry. Infections caused by Aspergillus spp. and Mucorales were also observed in the stomach, liver, and peritoneum. Furthermore, electron microscopy and DNA sequence analysis revealed multiple intranuclear inclusion bodies, identified as those produced by bovine adenovirus (BAdV) type 4, in various organs. This is the first report of C. albicans -induced encephalomyelitis along with BAdV type 4 infection in cattle. The etiology of death in this calf might be attributed to co-infections due to BAdV type 4, C. albicans , and multiple pathogenic species causing deep-seated mycosis.
{"title":"Co-infection of Deep-seated Mycosis caused by Candida albicans, Aspergillus spp., and Mucorales with Bovine Adenovirus Type 4 Infection in a Japanese Black Calf","authors":"Fumiko Suzuta, Yoshimi Terayama, Ryou Urakawa, Asuka Kumagai, K. Kimura","doi":"10.12935/jvma.75.e18","DOIUrl":"https://doi.org/10.12935/jvma.75.e18","url":null,"abstract":"A 4-day-old Japanese Black calf presented with fever as well as respiratory and neurological symptoms and died at the age of 17 days. Necropsy revealed multiple white nodules in the peritoneum and liver, erosions and ulcers in the forestomach and abomasum, and cerebrospinal fluid opacity. Microbiological examination was used to isolate Candida albicans from multiple organs, including the brain. The causative agent of the lesions was confirmed to be C. albicans using immunohistochemistry. Infections caused by Aspergillus spp. and Mucorales were also observed in the stomach, liver, and peritoneum. Furthermore, electron microscopy and DNA sequence analysis revealed multiple intranuclear inclusion bodies, identified as those produced by bovine adenovirus (BAdV) type 4, in various organs. This is the first report of C. albicans -induced encephalomyelitis along with BAdV type 4 infection in cattle. The etiology of death in this calf might be attributed to co-infections due to BAdV type 4, C. albicans , and multiple pathogenic species causing deep-seated mycosis.","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86484283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitsushi Kobayashi, Kouji Furuuchi, Jin Onodera, F. Koike, Yoshiriho Tsuji, M. Nagase, Y. Morita, H. Toyofuku
We compared total aerobic plate counts (APC) and Enterobacteriaceae counts on Japanese black cattle ( wagyu ) carcasses obtained by a destructive method and a sponge swabbing method. A total of 60 samples were collected by each method in both winter (January – March 2020) and summer (July – September 2020). Each sample obtained by the destructive method was 20 cm 2 (about 10 g) of carcass surface from four different parts (rump, top flank, bottom flank, and brisket), each of which were 5 cm 2 . The samples obtained by the sponge swabbing method were 400 cm 2 , swabbed at the surface of the same four parts, and were 100 cm 2 each. The mean ± standard deviation of the APC and Enterobacteriaceae counts obtained by the destructive method were 1.50 ± 0.79 log cfu/cm 2 and 0.44 ± 0.21 log cfu/cm 2 , respectively, while those obtained by the sponge swabbing method were 1.00 ± 0.48 log cfu/cm 2 and 0.29 ± 0.12 log cfu/cm 2 . APC obtained by the destructive sampling method were significantly higher than those obtained by the sponge swabbing method ( P ＜ 0.01). There were no sea-sonal differences in APC and Enterobacteriaceae counts between the two sampling methods. The linear regres-sion equation of APC obtained by the destructive and sponge swabbing methods was y ＝ 0.25 x ＋ 0.62, and the coefficient of determination (R 2 ) was 0.17. The correlation of APC between the destructive method and the sponge swabbing method seemed to be low.
采用破坏法和海绵拭子法对日本黑牛(和牛)尸体进行了好氧平板总计数(APC)和肠杆菌科计数的比较。在冬季(2020年1 - 3月)和夏季(2020年7 - 9月)，每种方法共采集60份样本。采用破坏法从胴体4个不同部位(臀部、上腹、下腹和胸脯)取5 cm 2的样品，每个样品20 cm 2(约10 g)。海绵拭子法获得的样品为400 cm 2，在相同的四个部分表面拭子，各为100 cm 2。破坏法APC和Enterobacteriaceae计数的平均值±标准差分别为1.50±0.79 log cfu/cm 2和0.44±0.21 log cfu/cm 2，海绵拭法APC和Enterobacteriaceae计数的平均值±标准差分别为1.00±0.48 log cfu/cm 2和0.29±0.12 log cfu/cm 2。破坏性取样法测得的APC显著高于海绵拭法(P < 0.01)。两种采样方法间APC和enterobacteraceae计数无海区差异。破坏法和海绵法测定APC的线性回归方程为y = 0.25 x + 0.62，决定系数(r2)为0.17。破坏法与海绵拭法APC的相关性较低。
{"title":"Comparison of Indicator Organism Counts on Beef Carcass Obtained by Destructive and Sponge Swabbing Methods","authors":"Mitsushi Kobayashi, Kouji Furuuchi, Jin Onodera, F. Koike, Yoshiriho Tsuji, M. Nagase, Y. Morita, H. Toyofuku","doi":"10.12935/jvma.75.e24","DOIUrl":"https://doi.org/10.12935/jvma.75.e24","url":null,"abstract":"We compared total aerobic plate counts (APC) and Enterobacteriaceae counts on Japanese black cattle ( wagyu ) carcasses obtained by a destructive method and a sponge swabbing method. A total of 60 samples were collected by each method in both winter (January – March 2020) and summer (July – September 2020). Each sample obtained by the destructive method was 20 cm 2 (about 10 g) of carcass surface from four different parts (rump, top flank, bottom flank, and brisket), each of which were 5 cm 2 . The samples obtained by the sponge swabbing method were 400 cm 2 , swabbed at the surface of the same four parts, and were 100 cm 2 each. The mean ± standard deviation of the APC and Enterobacteriaceae counts obtained by the destructive method were 1.50 ± 0.79 log cfu/cm 2 and 0.44 ± 0.21 log cfu/cm 2 , respectively, while those obtained by the sponge swabbing method were 1.00 ± 0.48 log cfu/cm 2 and 0.29 ± 0.12 log cfu/cm 2 . APC obtained by the destructive sampling method were significantly higher than those obtained by the sponge swabbing method ( P ＜ 0.01). There were no sea-sonal differences in APC and Enterobacteriaceae counts between the two sampling methods. The linear regres-sion equation of APC obtained by the destructive and sponge swabbing methods was y ＝ 0.25 x ＋ 0.62, and the coefficient of determination (R 2 ) was 0.17. The correlation of APC between the destructive method and the sponge swabbing method seemed to be low.","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"49 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89605445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yasuaki Inoue, H. Yaguchi, Miyuko Hiramatsu, Y. Wada
{"title":"Purulent Meningitis Due to Extraintestinal Pathogenic Escherichia coli with Omphalitis in a Neonatal Japanese Black Calf","authors":"Yasuaki Inoue, H. Yaguchi, Miyuko Hiramatsu, Y. Wada","doi":"10.12935/jvma.75.e51","DOIUrl":"https://doi.org/10.12935/jvma.75.e51","url":null,"abstract":"","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"196 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74494750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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