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A Preliminary Discussion on the Safety of Mild Therapeutic Hypothermia in Target Vessels after Endovascular Intervention in Acute Large Vessel Occlusion Cerebral Infarction. 急性大血管闭塞性脑梗死血管内介入治疗后靶血管亚低温治疗的安全性初探。
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2023-01-01 Epub Date: 2023-08-28 DOI: 10.1159/000532030
Jiang Li, Shaonian Tang, Juanli Liu, Wenlin He, Jinjin Yan, Zhiyong Huang, Xuesong Li

Introduction: The aim of this study was to discuss the safety of rapid administration of 4°C hypothermic normal saline into the occluded vessels using an intra-arterial catheter to induce mild hypothermia following endovascular thrombectomy in patients with acute large vessel occlusion cerebral infarction.

Methods: We selected 78 patients with acute large vessel occlusion cerebral infarction who underwent endovascular thrombectomy in the Department of Neurology of our hospital from January 2020 to July 2022 and achieved TICI 2b recanalization.

Result: Twenty-five patients were administered 500 mL of 4°C hypothermic normal saline in the occluded vessels at a rate of 25 mL/min to induce mild hypothermia. Twenty pairs of subjects conformed to strict matching and were finally included in the statistical analysis. The two groups of patients differed significantly in white blood cell count and percentage of neutrophils (p < 0.05); however, there were no significant differences in D-dimer, procalcitonin, and BNP levels. The two groups of patients did not differ significantly with respect to the incidence of the following indicators: upper gastrointestinal bleeding; pulmonary infection; venous thrombosis; vasospasms; seizures; and chills (p > 0.05).

Conclusion: Mild therapeutic hypothermia in target vessels plus endovascular thrombectomy was shown to be safe in patients with acute large vessel occlusion cerebral infarction.

引言:本研究的目的是讨论在急性大血管闭塞性脑梗死患者血管内血栓切除术后,使用动脉内导管将4°C低温生理盐水快速注入闭塞血管以诱导亚低温的安全性。方法:选择2020年1月至2022年7月在我院神经内科接受血管内血栓切除术并实现TICI 2b再通的78例急性大血管闭塞性脑梗死患者。结果:25名患者在闭塞血管中以25mL/min的速率给予500mL 4°C低温生理盐水,以诱导亚低温。20对受试者符合严格匹配,最终纳入统计分析。两组患者的白细胞计数和中性粒细胞百分比显著不同(p<0.05);然而,D-二聚体、降钙素原和BNP水平没有显著差异。两组患者在以下指标的发生率方面没有显著差异:上消化道出血;肺部感染;静脉血栓形成;血管痉挛;癫痫发作;结论:靶血管亚低温加血管内血栓切除术治疗急性大血管闭塞性脑梗死是安全的。
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引用次数: 0
Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress. Cx43促进剪切应力诱导的内皮细胞间充质转化。
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2023-01-01 Epub Date: 2023-09-06 DOI: 10.1159/000533320
En Zhou, Jing Zhou, Changlong Bi, Zongqi Zhang

Objectives: This study aimed to determine the function of Cx43 in the endothelial-to-mesenchymal transition (EndMT) process of endothelial cells (ECs) and to explore the potential signaling pathways underlying these functions.

Methods: ECs were extracted from rat aorta. ECs were transfected with Cx43 cDNA and Cx43 siRNA and then exposed to 5 or 12 dyne/cm2. Immunofluorescence staining was used to detect the expression of SM22α, Cx43, and acetylated α-tubulin in ECs. Western blotting was used to detect the protein expression of α-SMA, CD31, Cx43, H1-calponin, Ift88, and p-smad3 in ECs.

Results: The expression of αSMA, SM22α, and Cx43 was significantly increased, and CD31 was markedly decreased in ECs treated with laminar shear stress at 5 dyn/cm2. The Cx43 cDNA transfection could induce the expression of SM22α or H1-calponin and attenuate CD31 expression in ECs. Also, Cx43 overexpression harms cilia formation in ECs exposed to 5 dyn/cm2, accompanied with the regulated Ift88 and smad signaling.

Conclusions: This study found that laminar shear stress at 5 dyn/cm2 would increase the expression of Cx43 to facilitate the EndMT process of ECs, associated with morphological changes in primary cilia and the decreased expression of Ift88 in ECs.

目的:本研究旨在确定Cx43在内皮细胞(EC)内皮-间充质转化(EndMT)过程中的功能,并探索这些功能背后的潜在信号通路。方法:从大鼠主动脉中提取内皮细胞。用Cx43 cDNA和Cx43 siRNA转染EC,然后暴露于5或12达因/cm2。免疫荧光染色法检测内皮细胞中SM22α、Cx43和乙酰化α-微管蛋白的表达。采用蛋白质印迹法检测α-SMA、CD31、Cx43、H1钙蛋白酶、Ift88和p-smad3在内皮细胞中的蛋白表达。Cx43cDNA转染可诱导内皮细胞中SM22α或H1钙蛋白酶的表达,并减弱CD31的表达。此外,Cx43过表达损害暴露于5dyn/cm2的EC的纤毛形成,并伴有调节的Ift88和smad信号传导。结论:本研究发现,5dyn/cm2的层流剪切应力会增加Cx43的表达,以促进内皮细胞的EndMT过程,这与原发纤毛的形态学变化和内皮细胞中Ift88的表达减少有关。
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引用次数: 0
Thanks to the Reviewers 感谢审稿人
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-12-21 DOI: 10.1159/000527829

J Vasc Res 2022;59:394
[J]中国生物医学工程学报,2010;39 (3):391
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引用次数: 0
Front & Back Matter 正面和背面
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-10-01 DOI: 10.1159/000527666
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引用次数: 0
Front & Back Matter 正面和背面事项
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-07-01 DOI: 10.1159/000526085
C. Garland, A. Heagerty
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引用次数: 0
Perivascular Adipose Tissue Compensation for Endothelial Dysfunction in the Superior Mesenteric Artery of Female SHRSP.Z-Leprfa/IzmDmcr Rats 血管周围脂肪组织对雌性SHRSP.Z-Leprf/IzmDmcr大鼠肠系膜上动脉内皮功能障碍的补偿
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-04-29 DOI: 10.1159/000524187
S. Kagota, Risa Futokoro, Kana Maruyama-Fumoto, J. McGuire, K. Shinozuka
Regulation of arterial tone by perivascular adipose tissue (PVAT) differs between sexes. In male SHRSP.Z-Leprfa/IzmDmcr rats (SHRSP.ZF), PVAT exerts a compensatory relaxation effect for the loss of endothelium-mediated vasorelaxation, which occurs during the early stages of metabolic syndrome. However, this effect deteriorates by 23 weeks of age. Here, therefore, we compared the effects of PVAT in female and male SHRSP.ZF. Acetylcholine-induced relaxation in superior mesenteric artery without PVAT did not differ between 23-week-old females and males. However, the presence of PVAT enhanced relaxation in 23-week-old females, but not in males. The mRNA levels of angiotensin II type 1 receptor (AT1R) in PVAT did not differ between sexes, but AT1R-associated protein (ATRAP) and apelin levels were higher in females than in males. We observed a positive relationship between differences in artery relaxation with and without PVAT and ATRAP or apelin mRNA levels. In 30-week-old females, PVAT-enhanced relaxation disappeared, and mRNA levels of AT1R increased, while apelin levels decreased compared to 23-week-old females. These results demonstrated that in SHRSP.ZF, PVAT compensation for endothelium dysfunction extended to older ages in females than in males. Apelin and AT1R/ATRAP expression in PVAT may be predictors of favorable effects.
血管周围脂肪组织(PVAT)对动脉张力的调节因性别而异。在雄性SHRSP中。Z-Leprfa/IzmDmcr大鼠(SHRSP.ZF),PVAT对发生在代谢综合征早期的内皮介导的血管舒张的损失具有补偿性舒张作用。然而,这种影响在23周大时会恶化。因此,在这里,我们比较了PVAT对雌性和雄性SHRSP的影响。ZF。23周龄的女性和男性在没有PVAT的情况下,乙酰胆碱诱导的肠系膜上动脉舒张没有差异。然而,PVAT的存在增强了23周龄女性的放松,但男性没有。PVAT中血管紧张素II 1型受体(AT1R)的mRNA水平在性别之间没有差异,但女性的AT1R相关蛋白(ATRAP)和apelin水平高于男性。我们观察到有和没有PVAT的动脉舒张差异与ATRAP或apelin mRNA水平之间存在正相关。与23周龄女性相比,30周龄女性PVAT增强的松弛消失,AT1R的mRNA水平增加,而apelin水平下降。这些结果表明,在SHRSP中。ZF、PVAT对内皮功能障碍的补偿在女性中延伸到比男性更大的年龄。Apelin和AT1R/ATRAP在PVAT中的表达可能是有利效果的预测因素。
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引用次数: 1
In vivo Evidence of Arterial Dynamic Properties Alteration in Atherosclerotic Rabbit 动脉粥样硬化兔动脉动态特性改变的体内证据
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-04-19 DOI: 10.1159/000523898
C. Vayssettes-Courchay, C. Ragonnet, M. Isabelle, M. Bourguignon, S. Chimenti
Objectives: Atherosclerosis severely damages the arterial wall. The aim of this study was to assess in vivo, for the first time, arterial dynamic properties, reactivity, and stiffness in atherosclerotic (ATH) rabbits. Methods: The rabbits were fed with 0.3% cholesterol diet. Femoral artery (FA) or abdominal aorta (AA) diameter was recorded by echotracking, together with blood pressure. Arterial reactivity after local administration of agents and stiffness were measured as diameter or pulsatile diameter changes. Results: FA dilation induced by acetylcholine was reduced in the function of diet duration (9–65 weeks). With mid-term diet duration (35–45 weeks), the dilation to nitroprusside was greatly reduced; the constriction to norepinephrine was reduced but not that to serotonin, thromboxane agonist, or angiotensin II. After 17- and 28-week diet AA and FA stiffness were increased while distensibility was reduced. Arterial stiffness measured by regional pulse wave velocity was unaltered. We observed that after 28-week diet, FA exhibited a stiffened wall at the plaque level and higher distensibility at the upstream site. Discussion/Conclusion: Arterial reactivity and compliance were greatly modified by atherosclerosis, at various degrees dependent on diet duration. ATH rabbit is therefore a suitable model for in vivo investigations of treatments targeting dynamic properties of arterial wall.
目的:动脉粥样硬化严重损害动脉壁。本研究的目的是首次在体内评估动脉粥样硬化(ATH)兔的动脉动力学特性、反应性和硬度。方法:家兔采用0.3%胆固醇饮食。通过回声追踪记录股动脉(FA)或腹主动脉(AA)的直径以及血压。局部给药后的动脉反应性和硬度测量为直径或脉动直径变化。结果:乙酰胆碱诱导的FA扩张在饮食持续时间(9-65周)的功能中减少。随着中期饮食持续时间(35-45周),对硝普钠的扩张作用大大减少;去甲肾上腺素的收缩减少,但血清素、血栓素激动剂或血管紧张素II的收缩没有减少。在17和28周的饮食后,AA和FA硬度增加,而膨胀性降低。通过区域脉搏波速度测量的动脉硬度没有变化。我们观察到,在28周的饮食后,FA在斑块水平上表现出变硬的壁,在上游部位表现出更高的膨胀性。讨论/结论:动脉粥样硬化极大地改变了动脉反应性和顺应性,在不同程度上取决于饮食持续时间。因此,ATH兔是针对动脉壁动态特性进行体内治疗研究的合适模型。
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引用次数: 0
Establishment of Zebrafish Models for Diabetes Mellitus and Its Microvascular Complications 斑马鱼糖尿病及其微血管并发症模型的建立
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-04-04 DOI: 10.1159/000522471
Changsheng Chen, Dong Liu
Diabetes mellitus (DM) is a chronic metabolic disease known to cause several microvascular complications, including diabetic retinopathy, diabetic nephropathy, and diabetic neuropathy. Hyperglycemia plays a key role in inducing diabetic microvascular complications. A cohort of diabetic animal models has been established to study diabetes-related vascular diseases. However, the zebrafish model offers unique advantages in this field. The tiny size and huge offspring numbers of zebrafish make it amenable to perform large-scale analysis or screening. The easily accessible strategies for gene manipulation with morpholino or CRISPR/Cas9 and chemical/drug treatment through microinjection or skin absorption allow establishing the zebrafish DM models by a variety of means. In addition, the transparency of zebrafish embryos makes it accessible to perform in vivo high-resolution imaging of the vascular system. In this review, we focus on the strategies to establish diabetic or hyperglycemic models with zebrafish and the achievements and disadvantages of using zebrafish as a model to study diabetic microvascular complications.
糖尿病(DM)是一种慢性代谢性疾病,已知可引起多种微血管并发症,包括糖尿病视网膜病变、糖尿病肾病和糖尿病神经病变。高血糖是诱发糖尿病微血管并发症的关键因素。为了研究糖尿病相关血管疾病,建立了糖尿病动物模型。然而,斑马鱼模型在这一领域具有独特的优势。斑马鱼的体积小,后代数量多,这使得它适合进行大规模的分析或筛选。使用morpholino或CRISPR/Cas9进行基因操作以及通过显微注射或皮肤吸收进行化学/药物治疗的容易获得的策略允许通过多种手段建立斑马鱼DM模型。此外,斑马鱼胚胎的透明性使其能够在体内进行血管系统的高分辨率成像。本文就利用斑马鱼建立糖尿病或高血糖模型的策略以及利用斑马鱼作为模型研究糖尿病微血管并发症的成就和不足作一综述。
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引用次数: 4
Front & Back Matter 正面和背面
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-03-01 DOI: 10.1159/000524211
C. Wit, A. Heagerty
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引用次数: 0
A Novel ex vivo Method for Investigating Vascularization of Transplanted Islets. 一种研究移植胰岛血管形成的体外新方法。
IF 1.7 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-01-01 DOI: 10.1159/000523925
Robert Dolan, Arinola O Lampejo, Jorge Santini-González, Nicholas A Hodges, Edward A Phelps, Walter L Murfee

Revascularization of transplanted pancreatic islets is critical for survival and treatment of type 1 diabetes. Questions concerning how islets influence local microvascular networks and how networks form connections with islets remain understudied and motivate the need for new models that mimic the complexity of real tissue. Recently, our laboratory established the rat mesentery culture model as a tool to investigate cell dynamics involved in microvascular growth. An advantage is the ability to observe blood vessels, lymphatics, and immune cells. The objective of this study was to establish the rat mesentery tissue culture model as a useful tool to investigate islet tissue integration. DiI-labeled islets were seeded onto adult rat mesentery tissues and cultured for up to 3 days. Live lectin labeling enabled time-lapse observation of vessel growth. During culture, DiI-positive islets remained intact. Radial lectin-positive capillary sprouts with DiI labeling were observed to form from islets and connect to host networks. Lectin-positive vessels from host networks were also seen growing toward islets. PECAM and NG2 labeling confirmed that vessels sprouting from islets contained endothelial cells and pericytes. Our results introduce the rat mesentery culture model as a platform for investigating dynamics associated with the initial revascularization of transplanted islets.

移植胰岛的血运重建对1型糖尿病患者的生存和治疗至关重要。关于胰岛如何影响局部微血管网络以及网络如何与胰岛形成连接的问题仍未得到充分研究,并激发了对模拟真实组织复杂性的新模型的需求。最近,我们实验室建立了大鼠肠系膜培养模型,作为研究微血管生长过程中细胞动力学的工具。一个优点是能够观察血管、淋巴管和免疫细胞。本研究的目的是建立大鼠肠系膜组织培养模型,作为研究胰岛组织整合的有用工具。将dii标记的胰岛植入成年大鼠肠系膜组织,培养3天。活凝集素标记使血管生长的延时观察成为可能。在培养过程中,dii阳性的胰岛保持完整。观察到带有DiI标记的径向凝集素阳性毛细血管芽从胰岛形成并连接到宿主网络。来自宿主网络的凝集素阳性血管也向胰岛生长。PECAM和NG2标记证实,从胰岛冒出的血管含有内皮细胞和周细胞。我们的研究结果引入了大鼠肠系膜培养模型,作为研究移植胰岛初始血运重建相关动力学的平台。
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引用次数: 0
期刊
Journal of Vascular Research
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