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Thermophysical properties of 1D materials: Transient characterization down to atomic level 一维材料的热物理性质:原子水平的瞬态表征
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0098
Amin Karamati, Shen Xu, Huan Lin, Mahya Rahbar, Xinwei Wang
The thermophysical properties of 1D micro/nanoscale materials could differ significantly from those of their bulk counterparts due to intensive energy carrier scattering by structures. This work provides an in-depth review of cutting-edge techniques employed for transient characterization of thermophysical properties at the micro/nanoscale scale. In terms of transient excitation, step Joule heating, step laser heating, pulsed laser heating, and frequency domain amplitude-modulated laser heating are covered. For thermal probing, electrical and Raman scattering-based physical principles are used. These techniques enable the measurement of thermal conductivity, thermal diffusivity, and specific heat from the sub-mm level down to the atomic level (single-atom thickness). This review emphasizes the advantages of these techniques over steady state techniques and their physics, challenges, and potential applications, highlighting their significance in unraveling the intricate thermal transport phenomena to the atomic level of 1D materials.
由于结构对载流子的强烈散射,一维微纳米材料的热物理性质可能与块状材料有很大的不同。这项工作深入回顾了用于微/纳米尺度热物理性质瞬态表征的前沿技术。在瞬态激励方面,介绍了步进焦耳加热、步进激光加热、脉冲激光加热和频域调幅激光加热。对于热探测,使用基于电和拉曼散射的物理原理。这些技术可以测量从亚毫米级到原子级(单原子厚度)的热导率、热扩散率和比热。这篇综述强调了这些技术相对于稳态技术的优势,以及它们的物理、挑战和潜在的应用,强调了它们在将复杂的热输运现象揭示到一维材料的原子水平上的重要性。
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引用次数: 0
Modulating miRNA binding sites within circRNA for enhanced translation efficiency 调节circRNA内的miRNA结合位点以提高翻译效率
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0048
Kewei Zhang, Ge Shan, Liang Chen
Circular RNAs (circRNAs) are covalently closed circular RNAs, and some of them preserve translation potency. However, modulation of circRNA translation efficiency and its applications need to be explored. In this study, RNAs containing the translation initiation element CVB3 IRES and the coding sequence of luciferase protein were transcribed and circularized in vitro by T7 RNA polymerase and an optimized permutated intron‒exon (PIE) splicing strategy. The circularized RNAs were then transfected and translated into active luciferase in the cultured cells. Insertion of miRNA binding sites at the flanking region of the luciferase coding sequence significantly reduced the translation efficiency of the circRNAs. Mutations of the miRNA binding sites in the firefly luciferase coding sequence led to increased translation efficiency of synthetic circRNAs in cells. We also proved that mutations of the binding sites of specific miRNAs also enhanced the translation efficiency of synthetic circRNAs. Further in vivo experiments via bioluminescence imaging showed that synonymous mutation of the miRNA binding sites promoted synthetic circRNA translation in nude mice. This study demonstrates that the modulation of miRNA binding sites affects the translation efficiency of synthetic circRNAs in vitro and in vivo, which could be used as versatile tools for future applications in clinical imaging.
环状rna (circRNAs)是共价封闭的环状rna,其中一些保留翻译效力。然而,circRNA翻译效率的调控及其应用还有待探索。本研究利用T7 RNA聚合酶和优化的排列内含子-外显子(PIE)剪接策略,对含有翻译起始元件CVB3 IRES和荧光素酶蛋白编码序列的RNA进行体外转录和环化。然后转染环状rna并在培养细胞中翻译成活性荧光素酶。在荧光素酶编码序列的侧翼区域插入miRNA结合位点显著降低了circrna的翻译效率。萤火虫荧光素酶编码序列中miRNA结合位点的突变导致细胞中合成环状rna的翻译效率提高。我们还证明了特定mirna结合位点的突变也提高了合成环状rna的翻译效率。通过生物发光成像的进一步体内实验表明,miRNA结合位点的同义突变促进了裸鼠合成circRNA的翻译。本研究表明,miRNA结合位点的调节影响了体外和体内合成circRNAs的翻译效率,这可以作为未来临床成像应用的多功能工具。
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引用次数: 0
An abnormal multidrug-resistant and hypervirulent <i>Klebsiella pneumoniae</i> clinical isolate without <i>rmpA</i> or <i>rmpA2</i> 异常多重耐药和高毒肺炎克雷伯菌&lt;/i&gt;临床分离不含&lt;i&gt; rma &lt;或& lt; i&gt; rmpA2&lt; / i&gt;
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0085
Zhien He, Liwen Cao, Yuanyuan Dai, Huaiwei Lu, Baolin Sun, Yujie Li
Klebsiella pneumoniae is a notorious opportunistic pathogen, especially hypervirulent K. pneumoniae (hvKp). Fortunately, most classical hvKp strains are antibiotic-susceptible. However, in recent years, reports of multidrug-resistant hvKp (MDR-hvKp) have increased dramatically, threatening the health and safety of people worldwide. Here, we report the discovery of MDR-hvKp without rmpA and rmpA2 in a 92-year-old patient with chronic obstructive pulmonary disease. The patient died on the eighth day of hospitalization. Phenotyping experiments and whole-genome sequencing of K. pneumoniae isolate 21072329 isolated from the patient’s sputum were performed. Moreover, 21072329 belongs to ST11-KL47 MDR-hvKp, which was highly lethal to Galleria mellonella. Meanwhile, 21072329 had a strong viscosity, and it was difficult to completely centrifuge it; 21072329 carried ESBL genes (blaCTX-M-65, blaSHV-158, and blaTEM-1) and a carbapenemase gene (blaKPC-2), and it was resistant to carbapenem antibiotics and third- and fourth-generation cephalosporins. Although 21072329 had the characteristics of hvKp, rmpA and rmpA2 could not be found in its genome; it also only carried a siderophore of yersiniabactin. This may indicate that other hypervirulence factors promote the formation of hvKp. MDR-hvKp has already brought an enormous burden to global medical care, and those carrying unknown hypervirulence factors are new threats, so urgent prevention and control with research are urgently needed.
& lt; i>克雷伯氏菌pneumoniae< / i>是一种臭名昭著的机会致病菌,毒性特别强<& lt; i> pneumoniae< / i>(hvKp)。幸运的是,大多数经典hvKp菌株对抗生素敏感。然而,近年来,关于耐多药hvKp (MDR-hvKp)的报告急剧增加,威胁着全世界人民的健康和安全。在这里,我们报告发现了耐多药hvkp,没有<i>rmpA</i>和& lt; i> rmpA2< / i>一位92岁的慢性阻塞性肺病患者。患者在住院第8天死亡。K</i>表型实验及全基因组测序。& lt; i> pneumoniae< / i>从患者痰液中分离出21072329。此外,21072329属于ST11-KL47 MDR-hvKp,该菌株对mellonella</i>具有高致死率。同时,21072329黏度较强,难以完全离心;21072329携带ESBL基因(<i>bla</i><sub>CTX-M-65</sub> < bla</i> SHV-158</ i><sub>TEM-1</sub>)和碳青霉烯酶基因(<i>bla</i>< KPC-2</sub>),对碳青霉烯类抗生素和第三代、第四代头孢菌素耐药。虽然21072329具有hvKp的特性,但<i>rmpA</i>和& lt; i> rmpA2< / i>在其基因组中找不到;它也只携带了一个铁载体的yersinabactin。这可能表明其他高毒力因子促进了hvKp的形成。耐多药- hvkp已经给全球医疗保健带来了巨大负担,而那些携带未知高毒力因子的人是新的威胁,因此迫切需要进行紧急预防和控制研究。
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引用次数: 0
Predicting vapor-liquid equilibria of CO<sub>2</sub>+HFC binary mixtures by the PR EOS combined with a group contribution model 用PR - EOS结合群贡献模型预测CO&lt;sub&gt;2&lt;/sub&gt;+HFC二元混合物的汽液平衡
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0001
Zirui Wu, Lingfeng Shi, Rui Sun, Hua Tian, Xuan Wang, Gequn Shu
CO2+HFC binary mixtures have good performance and environmental friendliness and are considered good alternative working fluids in cooling and power cycle systems. The vapor-liquid phase equilibrium properties are key to the calculation of the enthalpy and entropy of mixtures, which is critical for the analysis of cooling and power cycle systems. To accurately predict the vapor-liquid equilibrium of CO2 and HFC (R23, R32, R41, R125, R134a, R143a, R152a, R161, and R227ea) binary mixtures, a group contribution model based on the excess free energy (GE) mixing rules (PR+MHV1+UNIFAC and PR+LCVM+UNIFAC) is established in this paper. The interaction parameters between groups such as -CO2, -Alkane, -CHF, and -CHF3 are obtained by the vapor-liquid phase equilibrium experiment of CO2 and HFC refrigerants, and these group parameters are critical for predicting their vapor-liquid phase equilibrium properties (the pressures and vapor phase molar fractions). The AARDp value calculated by the PR+LCVM+UNIFAC model is 5.53%, the value of AADy1 is 0.0132, and the AARDp and AADy1 values of the PR+MHV1+UNIFAC model are 7.40% and 0.0229, respectively. However, for the CO2+R32 system, the PR+MHV+UNIFAC prediction model can reproduce the experimental data with lower deviations, and the values of AARDp and AADy1 are 1.53% and 0.0045, respectively. In summary, for CO2+HFC binary mixtures, the PR+LCVM+UNIFAC group contribution model can reproduce the experimental data with lower deviations, but for individual CO2 binary mixtures (such as CO2+R32), the PR+MHV1+UNIFAC model also has unique advantages. According to the prediction results of the group contribution model, the PR+LCVM+UNIFAC model has significantly improved the calculation progress compared with the PR+MHV1+UNIFAC model used in the previous system.
CO<sub>2 /sub>+HFC二元混合物具有良好的性能和环境友好性,被认为是冷却和动力循环系统中良好的替代工作流体。气液相平衡性质是计算混合物焓和熵的关键,对冷却和动力循环系统的分析至关重要。准确预测CO<sub>2</sub>和HFC (R23、R32、R41、R125、R134a、R143a、R152a、R161、R227ea)二元混合物,建立了基于过量自由能(<i>G</i><sup>E</sup>)混合规则(PR+MHV1+UNIFAC和PR+LCVM+UNIFAC)的群体贡献模型。-CO<sub>2</sub>、-烷烃、-CHF和-CHF<sub>3</sub>由CO<sub>2</sub>和HFC制冷剂,这些基团参数对于预测它们的气液相平衡性质(压力和气相摩尔分数)至关重要。AARD< i>术中;/ i>PR+LCVM+UNIFAC模型计算的值为5.53%,AAD<i>y</i><sub>1</sub>为0.0132,AARD<i>p</i>和AAD< i> y< / i> & lt; sub> 1 & lt; / sub>PR+MHV1+UNIFAC模型的值分别为7.40%和0.0229。而对于CO<sub>2</sub>+R32系统,PR+MHV+UNIFAC预测模型能够以较小的偏差再现实验数据,且AARD<i>p</i>和AAD< i> y< / i> & lt; sub> 1 & lt; / sub>分别为1.53%和0.0045。综上所述,对于CO<sub>2& gt; /sub>+HFC二元混合物,PR+LCVM+UNIFAC群体贡献模型可以以较低的偏差重现实验数据,但对于单个CO<sub>2& gt; /sub>二元混合物(如CO<sub>2</sub>+R32), PR+MHV1+UNIFAC模型也具有独特的优势。从群体贡献模型的预测结果来看,PR+LCVM+UNIFAC模型与之前系统中使用的PR+MHV1+UNIFAC模型相比,计算进度明显提高。
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引用次数: 0
Design of novel double-layer wrapped ammonium polyphosphate and its application in aging-resistant and flame retardant crosslinked polyethylene composites 新型双层包覆聚磷酸铵的设计及其在耐老化阻燃交联聚乙烯复合材料中的应用
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0090
Pengfei Jia, Pengfei Sun, Fuhao Yu, Lei Song, Yuan Hu, Bibo Wang
In this study, double-layer wrapped ammonium polyphosphate (APP) is designed to enhance the mechanical properties, resistance and flame retardancy of crosslinked polyethylene (XLPE) composites. APP was wrapped with silica and then grafted with hindered phenol antioxidant 3-(3,5-di-tert-butyl-4 hydroxyphenyl) (AO) to prepare double-layer wrapped flame retardants (MCAPP). Due to the excellent compatibility between the MCAPP and XLPE matrix, the tensile strength and elongation at break of XLPE/MCAPP/CFA (XLPE-4) were improved. Moreover, the retention rate of elongation at break for the XLPE-4 composite reached 61.1%, significantly higher than that of XLPE-1 (2.6%) at 135 °C after aging for 14 d. This demonstrates that MCAPP could improve the aging resistance of XLPE cable composites. Compared with XLPE-1, the maximum smoke density and the peak heat release rate were reduced by 54.9% and 89.7%, respectively. Thus, the double-layer wrapping antioxidant strategy provides an excellent approach to obtain high-performance XLPE composites.
为了提高交联聚乙烯(XLPE)复合材料的力学性能、耐磨性和阻燃性,设计了双层包覆聚磷酸铵(APP)。用二氧化硅包覆APP,接枝受阻酚抗氧化剂3-(3,5-二叔丁基-4羟基苯基)(AO),制备双层包覆阻燃剂(MCAPP)。由于MCAPP与XLPE基体的良好相容性,XLPE/MCAPP/CFA (XLPE-4)的抗拉强度和断裂伸长率得到了提高。在135℃时效14 d后,XLPE-4复合材料的断裂伸长率达到61.1%,显著高于XLPE-1的2.6%,表明MCAPP可以提高XLPE电缆复合材料的耐老化性能。与XLPE-1相比,最大烟密度和峰值放热率分别降低了54.9%和89.7%。因此,双层包覆抗氧化策略为获得高性能XLPE复合材料提供了一种极好的方法。
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引用次数: 0
Cryopreservation of oocytes: history, achievements and future 卵母细胞的低温保存:历史、成就与未来
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0072
Shiyu Zhao, Gang Zhao
There have been increasing requirements for women’s fertility preservation due to oncological and nononcological reasons in recent years, and meeting these demands will be a hot topic in the coming years. Oocyte cryopreservation is a workable option for preserving women’s fertility, and great advances have already been made and much progress has been made in mammalian gene banking and human oocyte banks. In this paper, we systematically introduce the history of oocyte cryopreservation and vitrification technology and highlight the vitrification carrier. Furthermore, we summarize the fundamentals of oocyte vitrification and discuss the effects of vitrification on oocyte quality. Strategies to improve the effect of oocyte cryopreservation are also proposed. At the end of this review, we conclude oocyte cryopreservation and outline future perspectives.
近年来,由于肿瘤和非肿瘤的原因,对女性生育能力保存的要求越来越高,满足这些需求将是未来几年的一个热门话题。卵母细胞冷冻保存是保存妇女生育能力的可行选择,在哺乳动物基因库和人类卵母细胞库方面已经取得了很大进展。本文系统地介绍了卵母细胞冷冻和玻璃化技术的发展历史,重点介绍了玻璃化载体。此外,我们还概述了卵母细胞玻璃化的基本原理,并讨论了玻璃化对卵母细胞质量的影响。提出了提高卵母细胞低温保存效果的对策。最后,对卵母细胞低温保存技术进行了总结,并对其发展前景进行了展望。
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引用次数: 0
Effects of residual stress caused by abrasion on the flexoelectric response of BaTiO<sub>3</sub> ceramics 磨损产生的残余应力对BaTiO&lt;sub&gt;3&lt;/sub&gt;陶瓷
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0015
Xu Yang, Dongxia Tian, Xiaoyan Zhang, Ruzhong Zuo, Baojin Chu
The spontaneously polarized surface layer, which originates from stress relaxation, has been proposed for the unexpectedly large flexoelectric response measured in ferroelectric ceramics. However, the source of the stress that led to the polarized surface layer is still not completely known. In this work, the effect of surface stress on the microstructure, dielectric properties and flexoelectric response of BaTiO3 ceramics abraded by abrasive papers of various grit sizes was systematically studied. Compared with the as-prepared sample, the flexoelectric coefficients of abraded BaTiO3 ceramics decreased from ~600 μC/m to less than 200 μC/m. The flexoelectric coefficients of all the samples, however, recovered to ~500 μC/m following heat treatment at 200 °C and a subsequent slow cooling process. The results indicate that abrasion can introduce stress on the surface layers and affect the flexoelectric response of ferroelectric ceramics to some extent, but the stress is not the main reason for the formation of polarized surface layers.
自发极化表面层,它起源于应力松弛,已经提出了铁电陶瓷中测量到的意想不到的大挠曲电响应。然而,导致极化表面层的应力来源仍然不完全清楚。本文研究了表面应力对BaTiO<sub>3</sub>系统地研究了不同粒度的磨料纸对陶瓷的磨损。与制备样品相比,磨砂后的BaTiO<sub>3</sub>陶瓷从~600 μC/m降至小于200 μC/m。经过200℃热处理和缓慢冷却后,所有样品的挠曲电系数都恢复到~500 μC/m。结果表明,磨损会在铁电陶瓷的表层引入应力,并在一定程度上影响铁电陶瓷的挠曲电响应,但应力并不是导致表面极化层形成的主要原因。
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引用次数: 0
Attention: The impact of media attention on market reaction to corporate violations 关注度:媒体关注度对市场对企业违规行为反应的影响
Q4 Engineering Pub Date : 2023-01-01 DOI: 10.52396/justc-2023-0037
Chenxi Wang, Lei Zhou
Reducing market volatility and achieving high-quality development are important tasks for the Chinese capital market at the present stage. Based on the asset pricing role of media, this study used the event study to empirically examine the impact, as well as the heterogeneity from type and emotional tendency, of media attention on the market reaction to corporate violations from the perspective of limited attention. The results showed that the media’s prior attention to the listed company has a significantly negative impact on the market reaction after the company’s violation. The attention of network media and policy-oriented media has a significantly negative correlation with the market reaction after the company’s violation, while market-oriented media has no significant impact. Compared with neutral media attention, negative and positive media attention trigger more severe negative market reaction after company violations. Furthermore, the negative impact of media attention on the market reaction after corporate violations is mainly manifested in non-state-owned enterprises. The results demonstrate the important role of media attention in asset pricing and have important practical significance for better playing the role of the media, protecting the rights and interests of investors and achieving high-quality development of the capital market.
减少市场波动,实现高质量发展,是现阶段中国资本市场的重要任务。本研究基于媒体的资产定价作用,采用事件研究的方法,从有限关注的角度实证检验媒体关注对企业违规行为的市场反应的影响,以及媒体关注在类型和情绪倾向上的异质性。结果表明,媒体对上市公司的事前关注对公司违规后的市场反应有显著的负向影响。网络媒体和政策性媒体的关注度与公司违规后的市场反应呈显著负相关,而市场化媒体的关注度对公司违规后的市场反应无显著影响。与中性媒体关注相比,负面和正面媒体关注在公司违规后引发的市场负面反应更为严重。此外,媒体关注度对企业违规后市场反应的负面影响主要表现在非国有企业。研究结果证明了媒体关注度在资产定价中的重要作用,对于更好地发挥媒体作用,保护投资者权益,实现资本市场高质量发展具有重要的现实意义。
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引用次数: 0
Sound speed imaging of small animal organs by ultrasound computed tomography 小动物器官超声计算机断层成像的声速成像
Q4 Engineering Pub Date : 2022-01-01 DOI: 10.52396/justc-2021-0113
Zhiming Hu, Mingchun Yang, Xiang Zhu, Chao Tian
Sound speed is an important acoustic parameter for tissue characterization. Herein we developed an ultrasound computed tomography (USCT) system for ex vivo sound speed imaging and evaluation of small animal organs. The proposed USCT system employs a 256-element ring array transducer and allows simultaneous signal transmission and reception for all channels. The method does not require complicated sample preparation procedures and can yield accurate measurement results. Experimental results show that sound speeds of excised rat brain, heart, liver, spleen, and kidney measured by the method are close to published data. This work demonstrates a new method for sound speed imaging and holds potential for in vivo applications.
声速是组织表征的重要声学参数。在此,我们开发了一种超声计算机断层扫描(USCT)系统,用于小动物器官的离体声速成像和评估。所提出的USCT系统采用256单元环形阵列换能器,允许所有通道同时发送和接收信号。该方法不需要复杂的样品制备程序,可以产生准确的测量结果。实验结果表明,该方法测量的大鼠脑、心、肝、脾、肾的声速与已发表的数据接近。这项工作展示了一种新的声速成像方法,并具有在体内应用的潜力。
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引用次数: 1
Radiation hardness characterization of low gain avalanche detector prototypes for the high granularity timing detector 高粒度定时探测器低增益雪崩探测器样机的辐射硬度特性
Q4 Engineering Pub Date : 2022-01-01 DOI: 10.52396/justc-2021-0204
Xiao Yang, Kuo-Yan Ma, Xiangxuan Zheng, Yanwen Liu
The high granularity timing detector (HGTD) is a crucial component of the ATLAS phase II upgrade to cope with the extremely high pile-up (the average number of interactions per bunch crossing can be as high as 200). With the precise timing information (σt~30 ps) of the tracks, the track-to-vertex association can be performed in the “4-D” space. The Low Gain Avalanche Detector (LGAD) technology is chosen for the sensors, which can provide the required timing resolution and good signal-to-noise ratio. Hamamatsu Photonics K.K. (HPK) has produced the LGAD with thicknesses of 35 μm and 50 μm. The University of Science and Technology of China(USTC) has also developed and produced 50 μm LGADs prototypes with the Institute of Microelectronics (IME) of Chinese Academy of Sciences. To evaluate the irradiation hardness, the sensors are irradiated with the neutron at the JSI reactor facility and tested at USTC. The irradiation effects on both the gain layer and the bulk are characterized by I-V and C-V measurements at room temperature (20 ℃) or −30 ℃. The breakdown voltages and depletion voltages are extracted and presented as a function of the fluences. The final fitting of the acceptor removal model yielded the c-factor of 3.06×10−16 cm−2, 3.89×10−16 cm−2 and 4.12×10−16 cm−2 for the HPK-1.2, HPK-3.2 and USTC-1.1-W8, respectively, showing that the HPK-1.2 sensors have the most irradiation resistant gain layer. A novel analysis method is used to further exploit the data to get the relationship between the c-factor and initial doping density.
高粒度定时探测器(HGTD)是ATLAS II阶段升级的关键组件,用于应对极高的堆积(每个束交叉的平均相互作用数可能高达200)。利用轨迹的精确时序信息(σt~30 ps),可以在“四维”空间中实现轨迹与顶点的关联。传感器采用低增益雪崩检测器(LGAD)技术,可以提供所需的时序分辨率和良好的信噪比。Hamamatsu Photonics K.K. (HPK)生产了厚度为35 μm和50 μm的LGAD。中国科学技术大学(USTC)还与中国科学院微电子研究所(IME)合作开发并生产了50 μm lgad原型。为了评估辐照硬度,传感器在JSI反应堆设施上进行了中子辐照,并在中国科技大学进行了测试。通过室温(20℃)和- 30℃下的I-V和C-V测量来表征辐照对增益层和本体的影响。击穿电压和耗尽电压被提取出来并作为影响的函数表示出来。HPK-1.2、HPK-3.2和USTC-1.1-W8的受体去除模型最终拟合得到的c因子分别为3.06×10−16 cm−2、3.89×10−16 cm−2和4.12×10−16 cm−2,表明HPK-1.2传感器具有最耐辐照的增益层。采用一种新的分析方法对数据进行进一步挖掘,得到了c因子与初始掺杂密度之间的关系。
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引用次数: 0
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