Pub Date : 2022-11-15DOI: 10.51316/jst.162.etsd.2022.32.5.5
ZnO's ability to decompose organic substances is receiving much attention in multiple applications. ZnO has a broad spectrum of light absorption natural or synthetic, for a wide range of wavelengths, and is an important material with excellent properties, environmentally friendly, nontoxic, inexpensive, and high redox potential features with simple manufacturing methods. In this study, zinc oxide nanoparticles were synthesized by precipitation method with the participation of precursors (CH3COO)2Zn.2H2O and KOH. The surface morphology, chemical properties, and chemical composition of the ZnO catalyst were characterized by X-ray diffraction (XRD), Scanning Electron Microscopy, and Energy-Dispersive X-ray Spectroscopy (SEM-EDS). The photocatalytic activity of ZnO was evaluated with the color change of methylene blue (MB), methylene orange (MO), and diluted coffee solutions. The results show that ZnO has a high photocatalytic activity with a decomposition ability of over 90% for MO and MB pigments, and over 40% for a coffee solution when irradiated under ultraviolet light. This study's cotton fabric impregnated with ZnO showed good self-cleaning effects against organic pigments, which are MB, MO, and coffee. The results were demonstrated by the color changes over a 19-h period of time.
{"title":"Study to Evaluate Photocatalytic Decomposition of Several Organic Compounds and Self-Cleaning Ability of Fabrics Containing ZnO","authors":"","doi":"10.51316/jst.162.etsd.2022.32.5.5","DOIUrl":"https://doi.org/10.51316/jst.162.etsd.2022.32.5.5","url":null,"abstract":"ZnO's ability to decompose organic substances is receiving much attention in multiple applications. ZnO has a broad spectrum of light absorption natural or synthetic, for a wide range of wavelengths, and is an important material with excellent properties, environmentally friendly, nontoxic, inexpensive, and high redox potential features with simple manufacturing methods. In this study, zinc oxide nanoparticles were synthesized by precipitation method with the participation of precursors (CH3COO)2Zn.2H2O and KOH. The surface morphology, chemical properties, and chemical composition of the ZnO catalyst were characterized by X-ray diffraction (XRD), Scanning Electron Microscopy, and Energy-Dispersive X-ray Spectroscopy (SEM-EDS). The photocatalytic activity of ZnO was evaluated with the color change of methylene blue (MB), methylene orange (MO), and diluted coffee solutions. The results show that ZnO has a high photocatalytic activity with a decomposition ability of over 90% for MO and MB pigments, and over 40% for a coffee solution when irradiated under ultraviolet light. This study's cotton fabric impregnated with ZnO showed good self-cleaning effects against organic pigments, which are MB, MO, and coffee. The results were demonstrated by the color changes over a 19-h period of time.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79319241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-15DOI: 10.51316/jst.162.etsd.2022.32.5.8
When researching, designing and manufacturing a model of Co-combustion and gasification of biomass furnace with the goal of achieving the amount of air supplied for the combustion process, here the first stage is combustion/burning process, so there is excess oxygen, which means excess air > 1.The next stage is gasification, i.e. anaerobic combustion, so the oxygen content of the combustion process is anoxic, which means that the excess air is < 1. It is the combination of such simultaneous combustion and gasification that the energy conversion process will achieve higher thermal efficiency. In this report, we present a summary of experimental research results on the "model" of the furnace and simultaneous gasification, the results of evaluating the quality of the gas generated from the combustion process, the achieved flame temperature inside the furnace, the heat capacity of the furnace and the selection of the air flow provided for the combustion process. With the type of furnace and gasifier at the same time in this research, it can be used for drying agricultural products of household scale, or group of households, or used for medium scale at agricultural product processing establishments/enterprises and suitable for rural and/or mountainous conditions in Vietnam.
{"title":"Experimental Survey of Co-Combustion and Gasification of Biomass Furnace","authors":"","doi":"10.51316/jst.162.etsd.2022.32.5.8","DOIUrl":"https://doi.org/10.51316/jst.162.etsd.2022.32.5.8","url":null,"abstract":"When researching, designing and manufacturing a model of Co-combustion and gasification of biomass furnace with the goal of achieving the amount of air supplied for the combustion process, here the first stage is combustion/burning process, so there is excess oxygen, which means excess air > 1.The next stage is gasification, i.e. anaerobic combustion, so the oxygen content of the combustion process is anoxic, which means that the excess air is < 1. It is the combination of such simultaneous combustion and gasification that the energy conversion process will achieve higher thermal efficiency. In this report, we present a summary of experimental research results on the \"model\" of the furnace and simultaneous gasification, the results of evaluating the quality of the gas generated from the combustion process, the achieved flame temperature inside the furnace, the heat capacity of the furnace and the selection of the air flow provided for the combustion process. With the type of furnace and gasifier at the same time in this research, it can be used for drying agricultural products of household scale, or group of households, or used for medium scale at agricultural product processing establishments/enterprises and suitable for rural and/or mountainous conditions in Vietnam.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77237658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.10
This paper presents the most common methods of sheath bonding of transmission cables and the calculation of parameters, including rated voltage and energy absorption, of sheath voltage limiters for a mixed overhead-underground 220 kV transmission lines. The dependence of sheath voltage limiters parameters on the sheath types, system parameters such as the short-circuit capacity, the cable length, lightning current amplitudes, grounding resistance and cable installation are calculated in details. In this research, several methods in selecting sheath bonding types as well as sheath voltage limiters for a given set of conditions in mixed overhead-cable 220 kV transmission lines are proposed. The cross bonding permits to choose SVLs with the lowest rating voltage. However, the grounding resistance value of the tower at the junction between overhead lines and cables must be maintained at or below 3 Ω. The surrounding environment of cables changes, the required parameters of SVL to be selected must be recalculated to take the cable installation into account.
{"title":"Selection of Sheath Voltage Limiter for Mixed Overhead-Underground Cable in 220 kV Transmission Lines","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.10","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.10","url":null,"abstract":"This paper presents the most common methods of sheath bonding of transmission cables and the calculation of parameters, including rated voltage and energy absorption, of sheath voltage limiters for a mixed overhead-underground 220 kV transmission lines. The dependence of sheath voltage limiters parameters on the sheath types, system parameters such as the short-circuit capacity, the cable length, lightning current amplitudes, grounding resistance and cable installation are calculated in details. In this research, several methods in selecting sheath bonding types as well as sheath voltage limiters for a given set of conditions in mixed overhead-cable 220 kV transmission lines are proposed. The cross bonding permits to choose SVLs with the lowest rating voltage. However, the grounding resistance value of the tower at the junction between overhead lines and cables must be maintained at or below 3 Ω. The surrounding environment of cables changes, the required parameters of SVL to be selected must be recalculated to take the cable installation into account.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83984609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.5
One of the most interesting topics relevant to Portland cement that has attracted many researchers’ interest is finding the solution for enhancing the strength of Portland cement, especially strength in early age. Using additives for enhancing early strength of Portland cement is regarded as a good solution. In this study, a mixture of trisopropanolamine (TIPA) and triethanolamine (TEA) was added in cement in the range of 0.020-0.035% to investigate the effect of this mixture on the properties of Portland cement. The results showed that the fineness of cement with the combined additive (TIPA + TEA) was higher than that of an individual one (TEA) and that of ordinary cement. With a suitable amount of additive, the consistency increased, the setting time decreased, the strength of cement enhanced when compared with ordinary cement. Compressive strength of cement prepared with a combined additive (0.01% TIPA + 0.02% TEA) at 1 day of age was the highest (with an increase of 24.21%).
硅酸盐水泥最有趣的课题之一是如何提高硅酸盐水泥的强度，特别是早期强度，引起了许多研究者的兴趣。采用添加剂提高硅酸盐水泥的早期强度是一种较好的解决方案。本研究将三异丙醇胺(TIPA)和三乙醇胺(TEA)在0.020-0.035%范围内加入水泥中，研究该混合物对硅酸盐水泥性能的影响。结果表明，TIPA + TEA复合添加剂的水泥细度高于单一添加剂(TEA)和普通水泥;与普通水泥相比，掺入适量添加剂后，水泥的稠度提高，凝结时间缩短，强度增强。掺加0.01% TIPA + 0.02% TEA的水泥1日龄抗压强度最高，提高了24.21%。
{"title":"Effects of Triisopropanolamine and Triethanolamine on Some Properties of Portland Cement","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.5","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.5","url":null,"abstract":"One of the most interesting topics relevant to Portland cement that has attracted many researchers’ interest is finding the solution for enhancing the strength of Portland cement, especially strength in early age. Using additives for enhancing early strength of Portland cement is regarded as a good solution. In this study, a mixture of trisopropanolamine (TIPA) and triethanolamine (TEA) was added in cement in the range of 0.020-0.035% to investigate the effect of this mixture on the properties of Portland cement. The results showed that the fineness of cement with the combined additive (TIPA + TEA) was higher than that of an individual one (TEA) and that of ordinary cement. With a suitable amount of additive, the consistency increased, the setting time decreased, the strength of cement enhanced when compared with ordinary cement. Compressive strength of cement prepared with a combined additive (0.01% TIPA + 0.02% TEA) at 1 day of age was the highest (with an increase of 24.21%).","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73073919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.4
Cellulose, one of the renewable and biodegradable polymers, has been extensively studied as a raw material for a newly and fully bio-based hydrogel. The synthesis of bio-hydrogel is based on the dissolution of extracted cellulose from rice straw in tetrabutylphosphonium hydroxide (TBPH) solvent followed by the gelation using epichlorohydrin (ECH) crosslinkers. The success of the extraction of cellulose from rice straw was evaluated by characteristic peaks of cellulose in Fourier Transform Infrared Spectroscopy (FTIR) spectrum. The hydrogel formation mechanism was investigated in this article, as well as the evaluation of swelling properties under different temperature and pH conditions. As hydrogel exhibited thermal and pH sensitive behavior, the highest swelling capacity was found at pH 7.0 and 60 ºC. The characterization of hydrogel was examined by Fourier Transform Infrared Spectroscopy (FTIR) and thermal analysis (TGA), indicating that the rice straw derived cellulose hydrogel was cellulose type II, similar to others hydrogels. The morphology of extracted cellulose and hydrogel were investigated by Scanning Electron Microscope (SEM). The hydrogel exhibited porousity structure with very large pore size that surrounded by cellulose/ECH layers. The purity of the hydrogel was determined through the amount of water immersed in the hydrogel for one day by Liquid Nuclear Magnetic Resonance (NMR) for the residual TBPH determination.
{"title":"Influence of Temperature and pH Conditions on the Swelling Properties of Rice Straw Derived Cellulose Hydrogel","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.4","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.4","url":null,"abstract":"Cellulose, one of the renewable and biodegradable polymers, has been extensively studied as a raw material for a newly and fully bio-based hydrogel. The synthesis of bio-hydrogel is based on the dissolution of extracted cellulose from rice straw in tetrabutylphosphonium hydroxide (TBPH) solvent followed by the gelation using epichlorohydrin (ECH) crosslinkers. The success of the extraction of cellulose from rice straw was evaluated by characteristic peaks of cellulose in Fourier Transform Infrared Spectroscopy (FTIR) spectrum. The hydrogel formation mechanism was investigated in this article, as well as the evaluation of swelling properties under different temperature and pH conditions. As hydrogel exhibited thermal and pH sensitive behavior, the highest swelling capacity was found at pH 7.0 and 60 ºC. The characterization of hydrogel was examined by Fourier Transform Infrared Spectroscopy (FTIR) and thermal analysis (TGA), indicating that the rice straw derived cellulose hydrogel was cellulose type II, similar to others hydrogels. The morphology of extracted cellulose and hydrogel were investigated by Scanning Electron Microscope (SEM). The hydrogel exhibited porousity structure with very large pore size that surrounded by cellulose/ECH layers. The purity of the hydrogel was determined through the amount of water immersed in the hydrogel for one day by Liquid Nuclear Magnetic Resonance (NMR) for the residual TBPH determination.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84888649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.8
A polymer composite material consists of two different phases with very different mechanical properties. Thus, there is a shrinkage when a decrease in temperature appears. This paper focuses on the matrix shrinkage of a unidirectional polymer matrix composite under a temperature drop. A Rayleigh-Ritz method is used to rapidly determine the matrix displacement (matrix shrinkage) field of virgin samples (initial state, without thermo oxidation). Additionally, numerical simulations are also carried out. A comparison of maximum matrix shrinkages is carried out among the experiment measurement, the Rayleigh-Ritz method, and the numerical simulation method. The numerical results of the matrix displacement are compared to the experiment and the Rayleigh-Ritz method. There is a good correlation between the results obtained by the two methods. Then, an assessment of the reliability of numerical simulations is given. The numerical simulations are then used to analyze the evolution of stress along the different paths on the sample to predict the damage behavior.
{"title":"Study of the Matrix Shrinkage on a Polymer Matrix Composite under a Drop of Temperature","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.8","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.8","url":null,"abstract":"A polymer composite material consists of two different phases with very different mechanical properties. Thus, there is a shrinkage when a decrease in temperature appears. This paper focuses on the matrix shrinkage of a unidirectional polymer matrix composite under a temperature drop. A Rayleigh-Ritz method is used to rapidly determine the matrix displacement (matrix shrinkage) field of virgin samples (initial state, without thermo oxidation). Additionally, numerical simulations are also carried out. A comparison of maximum matrix shrinkages is carried out among the experiment measurement, the Rayleigh-Ritz method, and the numerical simulation method. The numerical results of the matrix displacement are compared to the experiment and the Rayleigh-Ritz method. There is a good correlation between the results obtained by the two methods. Then, an assessment of the reliability of numerical simulations is given. The numerical simulations are then used to analyze the evolution of stress along the different paths on the sample to predict the damage behavior.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89098170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.1
Biological superhydrophobic surfaces are relatively common in nature. Inspired by the superhydrophobic feature, plentiful materials with hydrophobic surfaces have been fabricated for various purposes such as anti-corrosion, anti-icing, self-cleaning, friction reduction, and oil-water separation. In the present work, we report a scalable, simple, and rapid method to fabricate superhydrophobic film on copper substrates via a simple route including a chemical etching step in sodium persulfate and sodium hydroxide solution and followed by a modification step in stearic acid solution. The effect of the etchants concentration as well as etching time was investigated exhaustively. From the obtained results it was found that when a copper plate was etched in an etching solution with low etchant concentration, the contact angle of copper was greatly influenced by etching time. In contrast, the etching time hardly affected the contact angle of the copper plate at the high concentration of the etchants. The superhydrophobic feature of the copper surface (with a contact angle larger than 150o) was only achieved in certain fabrication conditions. The superhydrophobic copper substrates demonstrated good anti-corrosion properties. The corrosion current density of superhydrophobic copper substrates was 85 times smaller than that of pristine ones when manufactured under optimal conditions. In addition, the as-prepared superhydrophobic copper mesh was used to successfully separate toluene from its aqueous mixture. The superhydrophobic copper has great potential for applications in oil-water separation and anti-corrosion in aqueous environments.
{"title":"Fabrication of Superhydrophobic Copper via Chemical Etching and Modification","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.1","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.1","url":null,"abstract":"Biological superhydrophobic surfaces are relatively common in nature. Inspired by the superhydrophobic feature, plentiful materials with hydrophobic surfaces have been fabricated for various purposes such as anti-corrosion, anti-icing, self-cleaning, friction reduction, and oil-water separation. In the present work, we report a scalable, simple, and rapid method to fabricate superhydrophobic film on copper substrates via a simple route including a chemical etching step in sodium persulfate and sodium hydroxide solution and followed by a modification step in stearic acid solution. The effect of the etchants concentration as well as etching time was investigated exhaustively. From the obtained results it was found that when a copper plate was etched in an etching solution with low etchant concentration, the contact angle of copper was greatly influenced by etching time. In contrast, the etching time hardly affected the contact angle of the copper plate at the high concentration of the etchants. The superhydrophobic feature of the copper surface (with a contact angle larger than 150o) was only achieved in certain fabrication conditions. The superhydrophobic copper substrates demonstrated good anti-corrosion properties. The corrosion current density of superhydrophobic copper substrates was 85 times smaller than that of pristine ones when manufactured under optimal conditions. In addition, the as-prepared superhydrophobic copper mesh was used to successfully separate toluene from its aqueous mixture. The superhydrophobic copper has great potential for applications in oil-water separation and anti-corrosion in aqueous environments.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83446449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.7
Dengue is a dangerous infectious disease affecting more than half of the global population living in areas at risk of dengue. This disease is caused by infection with dengue virus (DENV) through the bite of infected female mosquitos. Detection of the dengue virus infection could be performed by virus isolation, molecular biology methods, or by immunology methods. Non-structural protein 1 (NS1) has been considered as a diagnostic marker for detection of dengue virus infection. In this study, the full length NS1 region from DENV serotype 3 was cloned in vectors to generate the recombinant constructs of pCR::DENV-3 ns1 and pET::DENV-3 ns1. The rNS1 protein was expressed successfully in E. coli BL21(DE3) and confirmed by Western blot. Optimal conditions for expression of rNS1 were established. The highest level of protein expression was achieved at induction conditions of 0.05 mM IPTG inducer, 2% ethanol, and 37 oC for 4 hours. The rNS1 protein was successfully purified by immobilized metal affinity chromatography. Obtained pure rNS1 could be used for further studies in the development of vaccine and diagnostic tools.
登革热是一种危险的传染病，影响着生活在登革热危险地区的全球一半以上人口。该病由受感染雌蚊叮咬感染登革病毒(DENV)引起。登革热病毒感染的检测可通过病毒分离、分子生物学方法或免疫学方法进行。非结构蛋白1 (NS1)已被认为是检测登革病毒感染的诊断标志物。本研究将DENV血清型3的NS1全长区克隆到载体上，生成pCR::DENV-3 NS1和pET::DENV-3 NS1重组结构。rNS1蛋白在大肠杆菌BL21(DE3)中成功表达，并经Western blot证实。确定了rNS1的最佳表达条件。在0.05 mM IPTG诱导剂、2%乙醇、37℃培养4小时的诱导条件下，蛋白表达量达到最高。采用固定化金属亲和层析法成功纯化了rNS1蛋白。获得的纯rNS1可用于疫苗开发和诊断工具的进一步研究。
{"title":"Cloning and Expression of a Recombinant NS1 Antigen from a Dengue 3 Serotype Viral Isolate in Escherichia Coli","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.7","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.7","url":null,"abstract":"Dengue is a dangerous infectious disease affecting more than half of the global population living in areas at risk of dengue. This disease is caused by infection with dengue virus (DENV) through the bite of infected female mosquitos. Detection of the dengue virus infection could be performed by virus isolation, molecular biology methods, or by immunology methods. Non-structural protein 1 (NS1) has been considered as a diagnostic marker for detection of dengue virus infection. In this study, the full length NS1 region from DENV serotype 3 was cloned in vectors to generate the recombinant constructs of pCR::DENV-3 ns1 and pET::DENV-3 ns1. The rNS1 protein was expressed successfully in E. coli BL21(DE3) and confirmed by Western blot. Optimal conditions for expression of rNS1 were established. The highest level of protein expression was achieved at induction conditions of 0.05 mM IPTG inducer, 2% ethanol, and 37 oC for 4 hours. The rNS1 protein was successfully purified by immobilized metal affinity chromatography. Obtained pure rNS1 could be used for further studies in the development of vaccine and diagnostic tools.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88658794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.9
Surface enhanced raman scattering is interested for a variety of applications, especially in determining the presence of substances at very low concentrations, at the level of ppm, that is obtained by amplifying the raman scattering signal of adsorbent particles on metal surfaces or nanostructures. In this paper, we report on surface enhanced raman scattering substrates based on metal-film-coated silica nanoparticle monolayer. The silica nanoparticles having the diameter of 196 nm are assembled into close-packaged monolayer on silicon substrate by spin coating technique. The gap among the silica nanoparticles is tuned by HF vapor etching. The investigations on reflectance characteristic and raman spectra show that close- and non-close-packaged monolayers on silicon substrate covered by a thin gold layer can be used as surface enhanced raman scattering substrates.
{"title":"Metal-Film-Coated Silica Nanoparticle Monolayers for Application in Surface Enhanced Raman Scattering","authors":"","doi":"10.51316/jst.161.etsd.2022.32.4.9","DOIUrl":"https://doi.org/10.51316/jst.161.etsd.2022.32.4.9","url":null,"abstract":"Surface enhanced raman scattering is interested for a variety of applications, especially in determining the presence of substances at very low concentrations, at the level of ppm, that is obtained by amplifying the raman scattering signal of adsorbent particles on metal surfaces or nanostructures. In this paper, we report on surface enhanced raman scattering substrates based on metal-film-coated silica nanoparticle monolayer. The silica nanoparticles having the diameter of 196 nm are assembled into close-packaged monolayer on silicon substrate by spin coating technique. The gap among the silica nanoparticles is tuned by HF vapor etching. The investigations on reflectance characteristic and raman spectra show that close- and non-close-packaged monolayers on silicon substrate covered by a thin gold layer can be used as surface enhanced raman scattering substrates.","PeriodicalId":17641,"journal":{"name":"JST: Engineering and Technology for Sustainable Development","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82277456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-15DOI: 10.51316/jst.161.etsd.2022.32.4.3
This study analyzes the literature review of the techniques for estimating Height Equivalent Theoretical Plate (HETP) values for various packing structure models, ranging from theoretical to semi-empirical to shortcut methods. Packing structures of various meshes and shapes were studied on the purification of benzaldehyde from the mixture of benzaldehyde and cinnamaldehyde. The packing structures M-50, M-80, O-80, and S-80 were estimated using Fenske’s approach as 0.052, 0.053, 0.045, and 0.056, respectively. Experiment data and simulation result obtained by commercial software were utilized to validate the packing's HETP value. A pilot of the vacuum batch distillation system was fabricated including a column filled with packing structures. The results indicated that the HETP value between experiment and simulation is within 0.6 percent deviation. On the pilot scale, the results of the benzaldehyde purifying were validated in a batch distillation system with the O-80 packing structure. This system will be developed for the larger scale, and it will practical.
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