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Antioxidant Activity of Dewandaru Leaf (Eugenia Uniflora L.) Ethanol Extract and Determination of Total Flavonoid and Phenolic Content 德旺达叶的抗氧化活性研究乙醇提取及总黄酮、酚类含量测定
Pub Date : 2022-10-31 DOI: 10.35799/jis.v22i2.43913
Khoirul Anwar, Faridha Maera Lokana, A. Budiarti
Dewandaru (Eugenia uniflora L.) is a plant that has antioxidant activities. The compounds contained phenolic compounds and flavonoids.  This study aimed to test the antioxidant activity of the ethanol extract of Dewandaru leaves (Eugenia uniflora L.) with ABTS method and determine the content of flavonoid and total phenolic compounds. Ethanol extract of Dewandaru leaves was obtained by maceration using 96% ethanol. The ethanol extract of Dewandaru leaves identified the active compounds by FTIR (Fourier Transform Infra Red) and phytochemical screening. Ethanol extract of Dewandaru leaves was tested for its antioxidant activity using the ABTS method to obtaine the IC50 value and determined total flavonoid and phenolic content using UV/Vis spectrophotometry to obtain the levels (mg/g sample). The identification results show that the ethanol extract of Dewandaru leaves is a flavonoid compound of the dihydroflavonol type which has a bound OH functional group, aliphatic CH, C = O, C = C aromatic, C - O and aliphatic CH, and there are hydroxy groups on the C-3, C atoms. -5, and C-7. The results of phytochemical screening showed that the ethanol extract of Dewandaru leaves contains saponins, tannins, phenolics, and flavonoids. The ethanol extract of Dewandaru leaves has antioxidant activity with an IC50 value of 33.92 ppm and trolox with an IC50 value of 19.24 ppm. Based on the IC50 value, both are categorized very strong antioxidant activity. The total phenolic and total flavonoid levels were 327.52 mgGAE/g and 35.65 mgQE/g.Keywords: Antioxidant; Eugenia uniflora L.; flavonoid; phenolicAktivitas Antioksidan Ekstrak Etanol Daun Dewandaru (Eugenia Uniflora L.) serta Penetapan Flavonoid dan Fenolik TotalABSTRAKDewandaru (Eugenia uniflora L.) termasuk salah satu tanaman yang mempunyai khasiat antioksidan. Senyawa yang terkandung didalamnya antara lain senyawa fenolik dan flavonoid. Penelitian ini bertujuan untuk melakukan uji aktivitas antioksidan ekstrak etanol daun dewandaru (Eugenia uniflora L.) menggunakan metode ABTS dan menetapkan kandungan senyawa flavonoid dan fenolik total. Ekstrak etanol daun dewandaru diperoleh dengan cara maserasi menggunakan etanol 96 %. Ekstrak etanol daun dewandaru diidentifikasi senyawa aktif dengan FTIR (Fourier Transform Infra Red) dan skrinning fitokimia. Ekstrak etanol daun dewandaru diuji aktivitas antioksidan menggunakan metode ABTS sehingga diperoleh nilai IC50. Selanjutnya ekstrak etanol daun dewandaru ditetapkan kandungan flavonoid dan fenolik totalnya secara spektrofometri UV/Vis hingga diperoleh kadarnya (mg/g sampel). Hasil identifikasi menunjukkan ekstrak etanol daun dewandaru mengandung senyawa golongan flavonoid jenis dihidroflavonol yang mempunyai gugus fungsi OH terikat, CH alifatik, C = O, C = C aromatik, C – O dan CH alifatik, serta terdapat gugus hidroksi pada atom C-3, C-5, dan C-7. Hasil skrining fitokimia  menunjukkan bahwa ekstrak etanol daun dewandaru mengandung senyawa saponin, tanin, fenolik, d
德万达鲁(Eugenia uniflora L.)是一种具有抗氧化活性的植物。这些化合物含有酚类化合物和类黄酮。本研究旨在用ABTS法测定德万达叶乙醇提取物的抗氧化活性,并测定其总黄酮和总酚类化合物的含量。用96%的乙醇浸渍法获得了德旺达鲁叶的乙醇提取物。用傅里叶变换红外光谱(FTIR)和植物化学筛选等方法对芦笋叶乙醇提取物的活性成分进行了鉴定。采用ABTS法测定德万大叶乙醇提取物的抗氧化活性,得到IC50值;采用紫外/可见分光光度法测定总黄酮和酚类含量,得到其含量(mg/g样品)。鉴定结果表明,德万达叶乙醇提取物为二氢黄酮醇型类黄酮化合物,具有结合OH官能团、脂肪族CH、C = O、C = C芳香、C- O和脂肪族CH,且C-3、C原子上有羟基。-5和-7。植物化学筛选结果表明,德万大叶乙醇提取物中含有皂苷、单宁、酚类物质和黄酮类物质。德旺达鲁叶乙醇提取物的抗氧化活性IC50值为33.92 ppm,三洛克斯的IC50值为19.24 ppm。根据IC50值,两者都具有很强的抗氧化活性。总酚和总黄酮水平分别为327.52 mgGAE/g和35.65 mgQE/g。关键词:抗氧化剂;uniflora .;类黄酮;【摘要】独角龙珠(Eugenia Uniflora L.)总黄酮(Flavonoid dan Fenolik)。仙牙牙阳terkandung didalamnya antara躺仙牙牙牙丹黄酮。黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物。Ekstrak乙醇,dewandaru, diperoleh, dengan, maserasi, menggunakan乙醇,96%。用傅里叶变换红外光谱(FTIR)和皮膜氧化法鉴别了一种新型的膜氧化法。estrak乙醇,dewandaru diuji活性抗氧化和孟古那坎方法ABTS - 2 - 2 - 1 - 2 - 1 - 2 - 1。黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物(mg/g样品)。研究鉴定了龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮、龙舌兰类黄酮。哈西尔skrning fitokia menunjukkan bahwa ekstrak乙醇,dewanaru mengandung senyawa皂苷,单宁,黄酮,丹黄酮。estrak乙醇,dewandaru, meiliki活性,抗氧化,dengan nilai IC50 sebesar 33,92 ppm和trolox dengan nilai IC50 sebesar 19,24 ppm。Berdasarkan nilai IC50 keduanya didiategorikan memoriliki aktivitas antioksidan yang sangat kuat。Sedangkan kadar fenolik总seta类黄酮总sebesar 327,52 mgGAE/g dan 35,65 mgQE/g。Kata kunci: antioksidan;uniflora .;fenolik;类黄酮
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引用次数: 1
Fortification of Cocogurt Made From Coconut (Cocos nucifera L.) Milk With Yellow Yam (Ipomea batatas L.) Puree 用椰子(Cocos nucifera L.)制成的椰子强化剂黄山药牛奶(ipoma batatas L.)泥
Pub Date : 2022-10-23 DOI: 10.35799/jis.v22i2.43912
Cenny Sulastri Br. Pandiangan, M. Sumual, L. Mandey
This study aims to fortify yogurt from coconut milk (cocogurt) with yellow sweet potato puree and its effect on pH, viscosity, total lactic acid bacteria, and identify sensory properties of preference for taste, aroma, color, and texture. The method used in this research is the manufacture of yogurt starter, coconut milk, and yellow yam puree, the test parameters include acidity test (pH value), viscosity, total lactic acid bacteria and organoleptic test. This study used a completely randomized design (CRD) with 6 additional treatments of yellow yam puree: P0(0%), P1(5%), P2(10%), P3(15%), P4(20%), P5(25%). The results showed that fortification of cocogurt with yellow yam puree produced cocogurt with lower acidity (pH) (3.38-4.71), viscosity (4.46-6.85 cP) and total lactic acid bacteria (2,3 x 105 - 6.5 x 105 cfu/ml) is increasing. This study concluded that the best cocogurt treatment was found in the fortification of 5% yellow yam puree with a pH value of 4.39, viscosity 4.46 cP, total lactic acid bacteria 2.3 x 105 cfu/ml and sensory properties of preference for taste, aroma, color, texture shows the score of the results of the scale (6), namely likes.Keywords: cocogurt; coconut milk; yellow yam puree Fortifikasi Cocogurt Santan Kelapa (Cocos nucifera L.) Dengan Puree Ubi Jalar Kuning (Ipomea batatas L.)ABSTRAKPenelitian ini bertujuan untuk melakukan fortifikasi yogurt dari santan kelapa (cocogurt) dengan puree ubi jalar kuning dan pengaruhnya terhadap pH, viskositas, total bakteri asam laktat, dan mengidentifikasi sifat sensoris tingkat kesukaan rasa, aroma, warna, dan tekstur. Metode yang digunakan dalam penelitian ini adalah pembuatan starter yogurt, santan kelapa, dan puree ubi jalar kuning. Parameter uji meliputi uji derajat keasaman (nilai pH), viskositas, total bakteri asam laktat serta uji organoleptik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan penambahan puree ubi jalar kuning : P0(0%), P1(5%), P2(10%), P3(15%), P4(20%), P5(25%). Hasil penelitian menunjukan bahwa fortifikasi cocogurt dengan puree ubi jalar kuning menghasilkan cocogurt dengan derajat keasaman (pH) yang semakin rendah (4,71-3,38), viskositas (4,46-6,85 cP) dan total bakteri asam laktat (2,3 x 105-6,5 x 105 cfu/ml) semakin meningkat. Penelitian ini menyimpulkan perlakuan terbaik cocogurt terdapat pada fortifikasi puree ubi jalar kuning 5% dengan nilai pH 4,39, viskositas 4,46 cP, total bakteri asam laktat 2,3 x 105 cfu/ml dan sifat sensoris tingkat kesukaan rasa, aroma, warna, tekstur menunjukan skor hasil skala (6) yaitu suka.Kata kunci: cocogurt; puree ubi jalar kuning; santan kelapa
本研究旨在用黄薯泥强化椰奶酸奶(椰浆),并研究其对酸碱度、粘度、总乳酸菌的影响,以及对口感、香气、颜色和质地的偏好。本研究采用的方法是制作酸奶发酵剂、椰浆和黄山药泥,测试参数包括酸度测试(pH值)、粘度、总乳酸菌和感官测试。本研究采用完全随机设计(CRD),在黄山药泥中添加P0(0%)、P1(5%)、P2(10%)、P3(15%)、P4(20%)、P5(25%) 6个处理。结果表明,黄山药泥强化椰果后,椰果的酸度(pH)为3.38 ~ 4.71,黏度(4.46 ~ 6.85 cP)降低,总乳酸菌数(2,3 × 105 ~ 6.5 × 105 cfu/ml)增加。本研究得出最佳的椰浆处理为强化5%黄山药泥,pH值为4.39,粘度为4.46 cP,乳酸菌总数为2.3 × 105 cfu/ml,感官性能对口感、香气、颜色、质地的偏好表现为评分结果(6),即喜欢。关键词:cocogurt;椰奶;黄山药果浆登干果脯(ipooma batatas L.)摘要/ abstract摘要:槟榔果浆的酸碱度、黏度、总黏度、黏度、香气、香气、黏度、黏度、黏度、黏度、黏度、黏度、黏度。Metode yang digunakan dalam penelitian ini adalah pembuatan酸奶,santan kelapa, danpuree ubi jalar kuning。参数uji meliputi uji derajat keasaman (nilai pH), viskositas,总bakteri asam lakat serta uji organoleptik。Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan penambahan puree ubi jalar kuning: P0(0%), P1(5%), P2(10%), P3(15%), P4(20%), P5(25%)。Hasil penelitian menunjukan bahwa fortifikasi cocogurdengan puree ubi jalar kuning menghasilkan cocogurdengan derajat keasaman (pH) yang semakin rendah (4,71-3,38), viskositas (4,46-6,85 cP)和total bakteri asam laktat (2,3 × 105-6,5 × 105 cfu/ml) semakin meningkat。Penelitian ini menmeny pulpulkan perlakan terbaik椰香terdapat pata fortifikasi puree ubi jalarkuning 5%, dengan nilai pH 4,39, viskositas 4,46 cP,总bakterasam lakat 2,3 × 105 cfu/ml,以及传感器tingkat kesukaan rasa,香气,warna, tekstur menunjukan skor hasil skala (6) yitu suka。Kata kunci:椰子;昆宁辣椒泥;santan kelapa
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引用次数: 0
The Use of SWAT Model to predict Erosion and Sediment in the catchment area of Lake Tondano 利用SWAT模型预测通达诺湖集水区的侵蚀和泥沙
Pub Date : 2022-10-21 DOI: 10.35799/jis.v22i2.43814
J. Rombang, J. Kalangi, Meity R. Rantung
The Use of SWAT Model to Predict Erosion and Sediment in the Catchment Area of Lake Tondano. The objective of this research is to assess the accuracy of SWAT model to predict erosion and sediment in the catchment area of lake Tondano. A comprehensive hydrological model of SWAT is one of several models available to predict erosion dan sediment load. The validation of this model would be tested and it would be acceptable if Nash-Sutcliffe model Efficiency (NSE) coefficient was equal or greater than 0.4. The results in this experiment showed that values of NSE coefficient between sediment load observed and predicted by SWAT were between 0.4 and 0.9. Therefore, the use of SWAT model to predict erosion dan sediment in Tondano lake catchment area is satisfying and very good.Keywords: Catchment area; erosion; sedimentation; SWAT; NSE Penggunaan Model SWAT untuk Prediksi Erosi dan Sedimen di Area Tangkapan Air Danau Tondano AbstrakPenggunaan Model SWAT untuk Prediksi Erosi dan Sedimen di Area Tangkapan Air Danau Tondano. Penelitian ini bertujuan menguji keakuratan model SWAT dalam menduga erosi dan sedimen di area tangkapan air danau Tondano. SWAT (Soil Water Assesment Tool) merupakan model hidrologi yang komprehensif karena mempertimbangkan kondisi biofisik, iklim dan manajemen area tangkapan air dalam prediksi erosi dan sedimen. Penggunaan model SWAT untuk pendugaan erosi dan sedimen di area tangkapan air danau Tondano dapat diterima apabila hasil validasi sedimen terukur dan sedimen pendugaan menunjukkan koefisien NSE (Nash-Sutcliffe model Efficiency) lebih besar atau sama dengan 0,4. Penelitian ini menunjukkan bahwa model SWAT bisa digunakan untuk prediksi erosi dan sedimentasi di area tangkapan air danau Tondano karena koefisien NSE yang diperoleh pada 4 sungai yang bermuara di danau Tondano berkisar antara 0,4 sampai 0,9. Dengan demikian, pendugaan erosi dan sedimen di area tangkapan air danau Tondano dengan model SWAT dapat memberikan hasil yang memuaskan sampai sangat baik.Kata kunci: Area tangkapan air; erosi; sedimentasi; SWAT; NSE
利用SWAT模型预测通达诺湖集水区的侵蚀和泥沙。本研究的目的是评估SWAT模型在通达诺湖集水区预测侵蚀和泥沙的准确性。SWAT综合水文模型是目前预测侵蚀泥沙负荷的几种模型之一。如果Nash-Sutcliffe模型效率(NSE)系数等于或大于0.4,则该模型的有效性有待检验,可以接受。本试验结果表明,观测到的输沙量与SWAT预测的NSE系数在0.4 ~ 0.9之间。因此,利用SWAT模型对桐达诺湖流域侵蚀丹沙进行预测是令人满意的,效果很好。关键词:集水区;侵蚀;沉积;斯瓦特;彭古南模型SWAT untuk Prediksi Erosi dan Sedimen di Area Tangkapan Air Danau Tondano摘要彭古南模型SWAT untuk Prediksi Erosi dan Sedimen di Area Tangkapan Air Danau Tondano。Penelitian ini bertujuan menguji keakuratan模型SWAT dalam menduga erosi dan sedimen di area tangkapan air danau Tondano。SWAT(土壤水分评价工具)merupakan模型水文,水文综合,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文,水文。pengunaan模型SWAT untuk pendugaan erosi dan sedimen di area tangkapan air danau Tondano dapat diterima apabila hasil validasi sedimen terukur dan sedimen pendugaan menunjukkan koefisien NSE (ash- sutcliffe模型效率)lebih besar atau sama [j] .武汉:武汉理工大学,2004。Penelitian ini menunjukkan bahwa模型SWAT bisa digunakan untuk prediksi侵蚀和沉积,asi区域,tangkapan空气danau Tondano karena koefisien NSE yang diperoleh pada 4 sungai yang bermuara di danau Tondano berkisar antara 0,4 sampai 0,9。邓干德米基安,彭杜加安,罗斯,丹,沉积,迪地区,唐加潘,空气,丹,东达诺,邓干模型,特警队成员,坎,哈西,杨,穆阿斯坎,sampai sangat baik。Kata kunci:唐噶班地区空气;erosi;sedimentasi;斯瓦特;分析了无
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引用次数: 0
Antibacterial Activity of Secondary Metabolits Isolated from Mangrove Leaves Sonneratia alba 红树海桑叶次生代谢产物的抗菌活性研究
Pub Date : 2022-09-29 DOI: 10.35799/jis.v22i2.42332
V. Dotulong, L. Damongilala, D. Wonggo, L. A. Montolalu
Mangroves Sonneratia alb is a plants that grows in tidal areas with considerable environmental pressure. This condition causes the plant to synthesize secondary metabolites that have certain bioactivity to protect it self from destruction. The purpose of this study was to isolate antibacterial secondary metabolites from the mangrove leaves of  S.alba. The method applied was to isolate secondary metabolites from the ethyl setate fraction using column chromatography and identified the results of isolation with thin layer chromatography until a pure isolate was obtained. Pure isolates were characterized in structure using HNMR, IR and LC-MS spectroscopy. The resulting isolate in the form of methanol-soluble oil had the molecular formula C24H38O4 with the name Di(2-ethylhexyl)phthalate. The antibacterial activity of this compound was the same as the antibacterial activity of amoxicillin as a positive control, namely the diameter of the inhibition zone was 7 mm at a sample concentration of 0,21%.Aktivitas Antibakteri Metabolit Sekunder yang Diisolasi dari Daun Mangrove Sonneratia albaABSTRAKMangrove termasuk Sonneratia alba adalah tumbuhan yang tumbuh pada daerah pasang surut dengan tekanan lingkungan yang cukup besar, keadaan ini menyebabkan tumbuhan mangrove menyintesis metabolit sekunder yang mempunyai bioaktivitas tertentu untuk melindungi diri dari perusakan. Tujuan dari penelitian ini adalah mengisolasi metabolit sekunder antibakteri  dari daun mangrove  S.alba.. Metode yang digunakan adalah mengisolasi metabolit sekunder pada fraksi etil asetat menggunakan kromatografi kolom dan identifikasi hasil isolasi dengan kromatografi lapis tipis hingga didapatkan isolat murni. Isolat murni dikarakterisasi strukturnya menggunakan spektroskopi HNMR, IR dan LC-MS. Isolat yang dihasilkan berupa minyak yang larut dalam metanol mempunyai rumus molekul C24H38O4 dengan nama Di(2-etilhexil)fthalat. Aktivitas antibakteri dari senyawa ini  sama dengan aktivitas antibakteri amoxicilin sebagai kontrol positif yaitu diameter zona hambat sebesar 7 mm pada konsentrasi sampel 0,21%.Kata kunci: Aktivitas antibakteri; metabolit sekunder; Sonneratia alba
红树海桑是一种生长在环境压力较大的潮汐地区的植物。这种情况导致植物合成具有一定生物活性的次生代谢物,以保护自己免受破坏。本研究的目的是从红树叶中分离出抗菌次生代谢物。采用柱层析法从乙酸乙酯馏分中分离次生代谢物,薄层析法对分离结果进行鉴定,直至获得纯分离物。利用HNMR、IR和LC-MS对分离物进行了结构表征。得到的分离物为甲醇可溶油,分子式为C24H38O4,命名为邻苯二甲酸二(2-乙基己基)酯。该化合物的抑菌活性与阳性对照阿莫西林的抑菌活性相同,即在样品浓度为0.21%时,抑菌带直径为7 mm。红树海桑抗虫菌代谢研究进展红树海桑抗虫菌代谢研究进展红树海桑抗虫菌代谢研究进展红树海桑抗虫菌代谢研究进展红树抗细菌拮抗作用下赤霉素的代谢研究。对蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂、蒙古赤眼蜂进行了研究。用核磁共振(HNMR)、红外光谱(IR)和液相色谱质谱(LC-MS)分析。分离阳地哈希酮类化合物、阳地哈希酮类化合物、阳地哈希酮类化合物、阳地哈希酮类化合物。抗细菌活性菌达西雅瓦尼、丹参和抗细菌活性菌阿莫西林sebagai对照阳性,yyitu直径带hambat sebesar 7 mm,含konsentrasi样品0.21%。Kata kunci:抗虫活性菌;metabolit sekunder;Sonneratia阿尔巴
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引用次数: 0
Phytochemical Screening, Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi (Schleichera oleosa (Lour.) Oken) Bark 克山比(Schleichera oleosa, Lour)乙醇和甲醇提取物的植物化学筛选及总酚与抗氧化活性的关系奥肯)树皮
Pub Date : 2022-09-16 DOI: 10.35799/jis.v22i2.40091
Nur Alim, T. Hasan, R. Rusman, Jasmiadi Jasmiadi, Z. Zulfitri
Phenolic content has a role in antioxidant activity. The higher the phenolic content of a test sample, the higher the antioxidant activity, which is indicated by a smaller IC50 value. Methanol and 70% ethanol are solvents that have been proven to be widely used to extract total plant phenolic compounds. This study aims to determine the phytochemical content and the relationship of total phenolic content with antioxidant activity of ethanol and methanol extract of Schleichera oleosa (Lour.) Oken) bark using UV-Vis spectrophotometry. Schleichera oleosa (Lour.) Oken was extracted by maceration using 70% ethanol and methanol as solvent. Qualitative analysis of phytochemical compounds using specific reagents, analysis of total phenolic content, and antioxidant activity test using the DPPH method using UV-Vis spectrophotometry at 764 nm and 515 nm, respectively. The results of the qualitative test showed that the ethanol and the methanol extract was positive for alkaloids, flavonoids, and phenolics. The results of the analysis of total phenolic content and antioxidant activity showed that the 70% ethanol extract had a total phenolic content of 7.6829 mg gallic acid equivalent/g with IC50 28.5240 ppm and the methanol extract had a total phenolic content of  9.2057 mg gallic acid equivalent/g with IC50 1.6191 ppm. From these results, it was concluded that the higher the total phenolic content, the higher the antioxidant activity.Keywords: Antioxidant; Schlechera oleosa (Lour.) Oken.; total phenolic Skrining Fitokimia dan Hubungan Kadar Fenolik Total dengan Aktivitas Antioksidan Ekstrak Etanol dan Metanol Kulit Batang Kesambi (Schleichera oleosa (Lour.) Oken)ABSTRAKKadar fenolik memiliki peran terhadap aktivitas antioksidan. Semakin tinggi kadar fenolik suatu sampel uji maka aktivitas antioksidannya juga semakin tinggi, yang ditunjukkan dengan nilai IC50 yang lebih kecil. Pelarut metanol dan etanol 70% merupakan pelarut yang telah terbukti banyak digunakan untuk menarik senyawa fenolik total tanaman.  Penelitian ini bertujuan untuk menentukan kandungan fitokimia, hubungan kadar fenolik total dengan aktivitas antioksidan ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) menggunakan spektrofotometri UV-Vis. Kulit batang kesambi (Schleichera oleosa (Lour.) Oken) diekstraksi dengan cara maserasi menggunakan pelarut etanol 70% dan pelarut metanol. Analisis kualitatif senyawa fitokimia menggunakan pereaksi spesifik, analisis kadar fenolik total dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri UV-Vis masing-masing pada panjang gelombang 764 nm dan 515 nm. Hasil uji kualitatif menunjukkan bahwa ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) positif mengandung alkaloid, flavonoid dan fenolik. Hasil analisis kadar fenolik total dan uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol 70% memiliki kadar fenolik total sebesar 7,6829  mgGAE/g ekstrak d
酚含量对抗氧化活性有影响。样品中酚类物质含量越高,抗氧化活性越高,其IC50值越小。甲醇和70%乙醇是已被证明广泛用于提取植物总酚类化合物的溶剂。本研究旨在测定石竹醇提物和甲醇提物的植物化学成分及其总酚含量与抗氧化活性的关系。用紫外-可见分光光度法测定树皮。Schleichera oleosa (Lour)以70%乙醇和甲醇为溶剂,浸渍法提取Oken。采用专用试剂对植物化学成分进行定性分析,采用DPPH法对总酚含量进行分析,采用紫外-可见分光光度法分别在764 nm和515 nm处进行抗氧化活性测试。定性分析结果表明,乙醇和甲醇提取物对生物碱、黄酮类化合物和酚类物质均呈阳性。总酚含量及抗氧化活性分析结果表明,70%乙醇提取物总酚含量为7.6829 mg没食子酸当量/g, IC50为28.5240 ppm;甲醇提取物总酚含量为9.2057 mg没食子酸当量/g, IC50为1.6191 ppm。综上所述,总酚含量越高,抗氧化活性越强。关键词:抗氧化剂;石蜡(石蜡)奥肯。总酚类物质:总酚类物质、总酚类物质、总酚类物质、总酚类物质、总酚类物质【摘要】kadar fenliklikiliki是一种具有抗抑郁活性的记忆活动。2 . Semakin tinggi kadar fenolik suatu sample uji maka aktivitas antioksidannya juga Semakin tinggi, yang ditunjukkan dengan nilai IC50 yang lebih kecil。Pelarut甲醇和乙醇70% merupakan Pelarut yang telah terbukti banyak digunakan untuk menarik senyawa fenolik总tanaman。(1)总密度、总密度、总密度、总密度、总密度、总密度、总密度、总密度、总密度、总密度。蒙古纳坎紫外-可见分光光度法。Kulit batang kesambi (Schleichera oleosa)奥肯)diekstraksi dengan卡拉maserasi menggunakan pelarut etanol 70%丹pelarut metanol。用紫外-可见分光光度法、紫外-可见分光光度法、紫外-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法、紫外光-可见分光光度法。【翻译】【翻译】【翻译】【翻译】阳性成分为孟脑洞生物碱、黄酮类丹酚。Hasil分析kadar fenolik总油脂油脂7,6829 mgGAE/g ekstrak dengan nilai IC50 sebesar 28,5240 ppm,而kasktrak甲醇memiliki kadar fenolik总油脂9,2057 mgGAE/g ekstrak dengan nilai IC50 sebesar 1,6191 ppm。达里哈尔·特朗普(darhal terterbut disimpulse)——他似乎在做一件好事,而不是在做一件好事——总让一件好事变成一件好事。Kata kunci: Antioksidan;fenolik总;石蜡(石蜡)奥肯
{"title":"Phytochemical Screening, Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi (Schleichera oleosa (Lour.) Oken) Bark","authors":"Nur Alim, T. Hasan, R. Rusman, Jasmiadi Jasmiadi, Z. Zulfitri","doi":"10.35799/jis.v22i2.40091","DOIUrl":"https://doi.org/10.35799/jis.v22i2.40091","url":null,"abstract":"Phenolic content has a role in antioxidant activity. The higher the phenolic content of a test sample, the higher the antioxidant activity, which is indicated by a smaller IC50 value. Methanol and 70% ethanol are solvents that have been proven to be widely used to extract total plant phenolic compounds. This study aims to determine the phytochemical content and the relationship of total phenolic content with antioxidant activity of ethanol and methanol extract of Schleichera oleosa (Lour.) Oken) bark using UV-Vis spectrophotometry. Schleichera oleosa (Lour.) Oken was extracted by maceration using 70% ethanol and methanol as solvent. Qualitative analysis of phytochemical compounds using specific reagents, analysis of total phenolic content, and antioxidant activity test using the DPPH method using UV-Vis spectrophotometry at 764 nm and 515 nm, respectively. The results of the qualitative test showed that the ethanol and the methanol extract was positive for alkaloids, flavonoids, and phenolics. The results of the analysis of total phenolic content and antioxidant activity showed that the 70% ethanol extract had a total phenolic content of 7.6829 mg gallic acid equivalent/g with IC50 28.5240 ppm and the methanol extract had a total phenolic content of  9.2057 mg gallic acid equivalent/g with IC50 1.6191 ppm. From these results, it was concluded that the higher the total phenolic content, the higher the antioxidant activity.Keywords: Antioxidant; Schlechera oleosa (Lour.) Oken.; total phenolic Skrining Fitokimia dan Hubungan Kadar Fenolik Total dengan Aktivitas Antioksidan Ekstrak Etanol dan Metanol Kulit Batang Kesambi (Schleichera oleosa (Lour.) Oken)ABSTRAKKadar fenolik memiliki peran terhadap aktivitas antioksidan. Semakin tinggi kadar fenolik suatu sampel uji maka aktivitas antioksidannya juga semakin tinggi, yang ditunjukkan dengan nilai IC50 yang lebih kecil. Pelarut metanol dan etanol 70% merupakan pelarut yang telah terbukti banyak digunakan untuk menarik senyawa fenolik total tanaman.  Penelitian ini bertujuan untuk menentukan kandungan fitokimia, hubungan kadar fenolik total dengan aktivitas antioksidan ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) menggunakan spektrofotometri UV-Vis. Kulit batang kesambi (Schleichera oleosa (Lour.) Oken) diekstraksi dengan cara maserasi menggunakan pelarut etanol 70% dan pelarut metanol. Analisis kualitatif senyawa fitokimia menggunakan pereaksi spesifik, analisis kadar fenolik total dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri UV-Vis masing-masing pada panjang gelombang 764 nm dan 515 nm. Hasil uji kualitatif menunjukkan bahwa ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) positif mengandung alkaloid, flavonoid dan fenolik. Hasil analisis kadar fenolik total dan uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol 70% memiliki kadar fenolik total sebesar 7,6829  mgGAE/g ekstrak d","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78583321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Aktivitas Antioksidan Ekstrak dan Fraksi dari Daun Kamboja Putih (Plumeria alba L.) terhadap 1,1-Difenilpikrilhidrazin (DPPH) (Antioxidant Activity of Extracts and Fractions of White Cambodian Leaves (Plumeria alba L.) against 1,1-Diphenylpicrylhydrazine (DPPH))
Pub Date : 2022-09-13 DOI: 10.35799/jis.v22i2.43434
Aloysius Barry Anggoro, Erlien Lusy Wijaya, N. Elisa
Radikal bebas merupakan senyawa yang berperan dalam peningkatan resiko penyakit degeneratif yang mendominasi angka kematian di Indonesia. Antioksidan sebagai penangkal radikal bebas, salah satunya terdapat dalam daun kamboja. Daun kamboja putih dipilih karena relatif aman, ketersediaan yang melimpah, dan memiliki potensi sebagai antioksidan yang cukup menjanjikan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dari ekstrak, partisi dan fraksi dari daun kamboja putih. Metode ekstraksi yang dipakai adalah remaserasi menggunakan pelarut metanol, dilanjutkan dengan partisi menggunakan pelarut n-heksana, etil asetat dan air. Partisi dengan IC50 paling rendah atau memiliki aktivitas antioksidan tertinggi selanjutnya difraksinasi dengan kromatografi kolom. Penentuan aktivitas antioksidan dengan metode DPPH dilakukan pada panjang gelombang 516,3 nm. Hasil penelitian menunjukkan bahwa partisi etil asetat memiliki IC50 terendah sebesar 94,43 ppm dibandingkan dengan partisi lainnya, sedangkan fraksi 2 memiliki daya antioksidan tertinggi diikuti fraksi 3 dan fraksi 1 dengan IC50 berturut-turut sebesar 54,11 ppm; 77,79 ppm dan 84,07 ppm.Kata kunci: Antioksidan; kamboja putih; metode DPPH; profil kromatografiABSTRACTFree radicals are compounds that play a role in increasing the risk of degenerative diseases that dominate mortality rates in Indonesia. Antioxidants as an antidote to free radicals, one of which is found in frangipani leaves. White frangipani leaves were chosen because they are relatively safe, abundantly available, and have promising potential as antioxidants. This study aims to determine the antioxidant effect of extracts, partitions, fractions from white frangipani leaves, and the thin layer chromatographic profile of the partition with the highest antioxidant activity. The extraction method being used was maceration using methanol followed by partioning the crude extract using n-hexane, ethyl acetate and water solvents. The partition with the lowest IC50 or having the highest antioxidant activity is then fractionated using column chromatography. The determination of antioxidant activity using DPPH method was performed at 516.3 nm. The results showed that  the ethyl acetate partition had the lowest IC50 among other partitions (94.43 ppm), whereas fraction 2 had the highest antioxidant effect which was followed by fraction 3 and fraction 1 with IC50 respectively, 54.11 ppm; 77.79 ppm and 84.07 ppm.Keywords: Antioxidant; chromatography profil; DPPH method; white frangipani
自由基是一种导致印度尼西亚死亡率上升的退行性疾病风险的化合物。抗氧化剂作为一种自由式抗毒素,可以在柬埔寨树叶中找到。柬埔寨的白叶之所以被选择,是因为它们相对安全、丰富,而且具有足够的抗氧化剂潜力。本研究旨在确定柬埔寨白叶的抗氧化活性、活性和成分。提取方法是利用甲醇溶剂,然后用n-heksana溶剂、醋酸乙和水进行分区处理。IC50的parti分最低或有抗氧化剂活性较高,然后由色谱谱表分析。DPPH方法的抗氧化活性测定发生在516.3 nm波长。研究结果表明,乙基乙基分区比其他分区低94.43 ppm,而2的抗氧化剂浓度最高,3的抗氧化剂和1的ppm共54.11 ppm;77.79 ppm和84.07 ppm。关键词:抗氧化剂;柬埔寨白色;DPPH方法;宗谱分析是一种犯罪行为,它增加了印尼潜在死亡率的风险。美国Antioxidants解药到自由的激进分子,一个无关紧要的是发现在乳香树叶。怀特乳香树叶是被选中的人,因为他们是relatively安全,abundantly "可以,和有promising antioxidants潜在的美国。这些研究表明,我们可以确定目前使用的提取方法是用n-hexane、ethyl aceyl和water solvents跟进的甲烷。与西方大多数IC50或有最先进的抗氧化剂反应,然后用合成色素图形分解。使用DPPH方法的反毒行动的确定在516.3 nm获胜。最近的结果表明,ethyl aceyl partition有较低的IC50和其他部分(94.43 ppm),其中2有最严重的抗氧化剂影响,其后果是由IC50表现为,54.11 ppm;77.79 ppm和84.07 ppm。安装:Antioxidant;chromatography简介;DPPH方法;怀特乳香
{"title":"Aktivitas Antioksidan Ekstrak dan Fraksi dari Daun Kamboja Putih (Plumeria alba L.) terhadap 1,1-Difenilpikrilhidrazin (DPPH) (Antioxidant Activity of Extracts and Fractions of White Cambodian Leaves (Plumeria alba L.) against 1,1-Diphenylpicrylhydrazine (DPPH))","authors":"Aloysius Barry Anggoro, Erlien Lusy Wijaya, N. Elisa","doi":"10.35799/jis.v22i2.43434","DOIUrl":"https://doi.org/10.35799/jis.v22i2.43434","url":null,"abstract":"Radikal bebas merupakan senyawa yang berperan dalam peningkatan resiko penyakit degeneratif yang mendominasi angka kematian di Indonesia. Antioksidan sebagai penangkal radikal bebas, salah satunya terdapat dalam daun kamboja. Daun kamboja putih dipilih karena relatif aman, ketersediaan yang melimpah, dan memiliki potensi sebagai antioksidan yang cukup menjanjikan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dari ekstrak, partisi dan fraksi dari daun kamboja putih. Metode ekstraksi yang dipakai adalah remaserasi menggunakan pelarut metanol, dilanjutkan dengan partisi menggunakan pelarut n-heksana, etil asetat dan air. Partisi dengan IC50 paling rendah atau memiliki aktivitas antioksidan tertinggi selanjutnya difraksinasi dengan kromatografi kolom. Penentuan aktivitas antioksidan dengan metode DPPH dilakukan pada panjang gelombang 516,3 nm. Hasil penelitian menunjukkan bahwa partisi etil asetat memiliki IC50 terendah sebesar 94,43 ppm dibandingkan dengan partisi lainnya, sedangkan fraksi 2 memiliki daya antioksidan tertinggi diikuti fraksi 3 dan fraksi 1 dengan IC50 berturut-turut sebesar 54,11 ppm; 77,79 ppm dan 84,07 ppm.Kata kunci: Antioksidan; kamboja putih; metode DPPH; profil kromatografiABSTRACTFree radicals are compounds that play a role in increasing the risk of degenerative diseases that dominate mortality rates in Indonesia. Antioxidants as an antidote to free radicals, one of which is found in frangipani leaves. White frangipani leaves were chosen because they are relatively safe, abundantly available, and have promising potential as antioxidants. This study aims to determine the antioxidant effect of extracts, partitions, fractions from white frangipani leaves, and the thin layer chromatographic profile of the partition with the highest antioxidant activity. The extraction method being used was maceration using methanol followed by partioning the crude extract using n-hexane, ethyl acetate and water solvents. The partition with the lowest IC50 or having the highest antioxidant activity is then fractionated using column chromatography. The determination of antioxidant activity using DPPH method was performed at 516.3 nm. The results showed that  the ethyl acetate partition had the lowest IC50 among other partitions (94.43 ppm), whereas fraction 2 had the highest antioxidant effect which was followed by fraction 3 and fraction 1 with IC50 respectively, 54.11 ppm; 77.79 ppm and 84.07 ppm.Keywords: Antioxidant; chromatography profil; DPPH method; white frangipani","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89968209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inventarisasi Tanaman Puring (Codiaeum variegatum L.) di Pekarangan Rumah Desa Makaaruyen, Kecamatan Modoinding, Kabupaten Minahasa Selatan, Provinsi Sulawesi Utara (Inventory of Croton Plant (Codiaeum variegatum L.) in the Backyard of Makaaruyen Village, Modoinding District, South Minahasa Regency,
Pub Date : 2022-09-02 DOI: 10.35799/jis.v22i2.41364
Christia Novindy Bororing, S. Mambu, S. Nio
Tanaman puring (Codiaeum variegatum L.) dikenal sebagai tanaman hias karena bentuk dan warna daun yang beragam. Penelitian ini bertujuan untuk menginventarisasi jenis-jenis puring yang dibudidayakan di pekarangan rumah Desa Makaaruyen, Kecamatan Modoinding, Kabupaten Minahasa Selatan. Pengambilan data dilakukan dengan metode survei langsung dengan menjelajah setiap pekarangan yang mempunyai tanaman puring, mengambil dokumentasi berupa foto, mengamati morfologi tanaman serta mencatat jumlah tanaman untuk setiap jenis tanaman puring yang ditemukan. Bedasarkan hasil penelitian terdapat 25 jenis puring yang terdapat di 13 titik lokasi penelitian, yaitu tokek merah, bor merah, caldium norma, sayap nuri, lele, red gems, gelatik, mentimun, bor emas, patricia, lele kirmizi, walet merah, apel malang, dasi merah, kecapi, lele redline, walet, merah, cabai, kamboja, spageti three color, batang merah, serit, kelabang, dan jet amerika. Puring gelatik paling banyak dibudidayakan dibandingkan dengan jenis puring yang lain.Kata kunci: Desa Makaaruyen; gelatik; Puring; Minahasa SelatanABSTRACTCroton (Codiaeum variegatum L.) is known as an ornamental plant because of its various leaf shapes and colors. This study aimed to inventory the types of croton that are cultivated in the home yard of Makaaruyen Village, Modoinding District, South Minahasa Regency. Data collection was conducted by direct survey method by exploring every yard with croton plants, collecting photo documentation, observing plant morphology, and recording the number of each croton plants. Based on the results of the study, there were 25 types of puring found in 13 research locations, namely tokek merah, bor merah, caldium norma, sayap nuri, lele, red gems, gelatik, mentimun, bor emas, patricia, lele kirmizi, walet merah, apel malang, dasi merah, kecapi, lele redline, walet, merah, cabai, kamboja, spageti three color, batang merah, serit, kelabang, dan jet amerika. Gelatik was the most widely cultivated compared to other types of croton.Keywords: Croton; gelatik; Makaaruyen Village; South Minahasa
凤梨科植物(学名Codiaeum,新品种)因其叶子的形状和颜色不同而为人所知。本研究旨在盘点croton栽培的品种Makaaruyen村,街道Modoinding家的院子里,南米纳哈萨县。直接与探索数据检索方法进行调查的每一个院子有了植物croton拍纪录片的照片,观察植物形态学和记录发现的每一种植物croton植物数量。肯定的研究成果有25种见于13点的croton壁虎的研究地点,即红、红钻头caldium规范翅膀的鹦鹉,乐乐,红色gems山雀、黄瓜、金钻帕特里夏,乐乐朱红,可怜的燕子,红苹果、红领带,竖琴,乐乐redline雨燕、红辣椒、柬埔寨三个颜色,红色的树干,serit意面、蜈蚣和美国飞机。croton山雀与其他类型的croton相比种植最多。关键词:Makaaruyen村;山雀;croton;如SelatanABSTRACTCroton (Codiaeum头L .)是美国知名的装饰植物,因为它的不同叶的形状变成和颜色。这一研究表明,在南米纳哈沙摄政区马卡鲁因村的家中发现了被占领的克罗顿村的特征。收藏was conducted由直接测量方法对数据探索每码与croton植物,muensterberger photo documentation, observing普兰特morphology当家》和录音,每croton植物。改编自《results of the study,那里是25 types of research locations croton发现在13日,namely红色,红色的钻头,壁虎caldium翅膀的鹦鹉,乐乐,红色gems规范山雀、黄瓜、金钻帕特里夏,乐乐朱红,可怜的燕子,红苹果、红领带,竖琴,乐乐redline雨燕、红辣椒、柬埔寨三个颜色,红色的树干,serit意面、蜈蚣和美国飞机。山雀是《头号widely cultivated compared to另types of croton。安装:Croton;山雀;Makaaruyen村;南米纳哈萨
{"title":"Inventarisasi Tanaman Puring (Codiaeum variegatum L.) di Pekarangan Rumah Desa Makaaruyen, Kecamatan Modoinding, Kabupaten Minahasa Selatan, Provinsi Sulawesi Utara (Inventory of Croton Plant (Codiaeum variegatum L.) in the Backyard of Makaaruyen Village, Modoinding District, South Minahasa Regency,","authors":"Christia Novindy Bororing, S. Mambu, S. Nio","doi":"10.35799/jis.v22i2.41364","DOIUrl":"https://doi.org/10.35799/jis.v22i2.41364","url":null,"abstract":"Tanaman puring (Codiaeum variegatum L.) dikenal sebagai tanaman hias karena bentuk dan warna daun yang beragam. Penelitian ini bertujuan untuk menginventarisasi jenis-jenis puring yang dibudidayakan di pekarangan rumah Desa Makaaruyen, Kecamatan Modoinding, Kabupaten Minahasa Selatan. Pengambilan data dilakukan dengan metode survei langsung dengan menjelajah setiap pekarangan yang mempunyai tanaman puring, mengambil dokumentasi berupa foto, mengamati morfologi tanaman serta mencatat jumlah tanaman untuk setiap jenis tanaman puring yang ditemukan. Bedasarkan hasil penelitian terdapat 25 jenis puring yang terdapat di 13 titik lokasi penelitian, yaitu tokek merah, bor merah, caldium norma, sayap nuri, lele, red gems, gelatik, mentimun, bor emas, patricia, lele kirmizi, walet merah, apel malang, dasi merah, kecapi, lele redline, walet, merah, cabai, kamboja, spageti three color, batang merah, serit, kelabang, dan jet amerika. Puring gelatik paling banyak dibudidayakan dibandingkan dengan jenis puring yang lain.Kata kunci: Desa Makaaruyen; gelatik; Puring; Minahasa SelatanABSTRACTCroton (Codiaeum variegatum L.) is known as an ornamental plant because of its various leaf shapes and colors. This study aimed to inventory the types of croton that are cultivated in the home yard of Makaaruyen Village, Modoinding District, South Minahasa Regency. Data collection was conducted by direct survey method by exploring every yard with croton plants, collecting photo documentation, observing plant morphology, and recording the number of each croton plants. Based on the results of the study, there were 25 types of puring found in 13 research locations, namely tokek merah, bor merah, caldium norma, sayap nuri, lele, red gems, gelatik, mentimun, bor emas, patricia, lele kirmizi, walet merah, apel malang, dasi merah, kecapi, lele redline, walet, merah, cabai, kamboja, spageti three color, batang merah, serit, kelabang, dan jet amerika. Gelatik was the most widely cultivated compared to other types of croton.Keywords: Croton; gelatik; Makaaruyen Village; South Minahasa","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90839700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Penerapan Metode SARIMA dalam Model Intervensi Fungsi Step untuk Memprediksi Jumlah Pegunjung Objek Wisata Londa (Application of the SARIMA Method in the Step Function Intervention to Predict the Number of Visitors at Londa Tourism Object)
Pub Date : 2022-08-28 DOI: 10.35799/jis.v22i2.40961
Geovani Christie, Djoni Hatidja, Rinancy Tumilaar
Tujuan dari penelitian ini yaitu menentukan model intervensi pada data jumlah pengunjung di objek wisata Londa dan memprediksi jumlah pengunjung objek wisata Londa pada bulan Desember 2021 sampai Juni 2022 menggunakan model Seasonal Autoregresive Integrated Moving Average (SARIMA). Data yang digunakan adalah data jumlah pengunjung bulanan pada periode bulan Januari 2018 sampai bulan November 2021, yang diperoleh dari pihak pengelolah objek wisata Londa.  Pola data jumlah pengunjung pada objek wisata Londa mengalami fluktuasi yang signifikan, yaitu pada bulan April 2020 (T=28). Model intervensi yang digunakan dalam penelitian ini yaitu fungsi step karena pengaruh yang terjadi berlangsung dalam kurun waktu cukup lama yaitu pada bulan April 2020 sampai November 2021 (T=28 sampai T=47).  Model terbaik yang diperoleh dari hasil analisis data yaitu SARIMA (1,1,0)(1,1,0)[6] dengan orde intervensi b=0, s=5, dan r=2, dengan nilai MAPE dan MAE masing-masing sebesar 4,38% dan 0,397.  Hasil prediksi dari bulan Desember 2021 sampai Juni 2022 secara berturut-turut yaitu 2550, 756, 347, 515, 1585, 1287 dan 2247 orang.  Jumlah pengunjung akan meningkat pada waktu musim liburan yaitu Bulan Desember dan Juni.Kata kunci: Model intervensi; fungsi step; objek wisata Londa,ABSTRACTThe object of this study are to determine the intervention model of the number of visitors at the Londa tourism object and predict the number of visitors at the Londa tourism object for December 2021 to June 2020 using Seasonal Autoregresive Integrated Moving Average (SARIMA) models. The data used is monthly data for the period from January 2018 to November 2021, which was obtained from the manager of the Londa tourist object. The data pattem on the number of visitors at the Londa tourism object experienced significant fluctuations on April 2020 (T=28). The suspected intervention model is a step function because the effect of the intervention that occurred took place over a long period of time, from April 2020 to November 2021 (T=28 to T=47). The best model obtained from the results of data analysis is SARIMA (1,1,0)(1,1,0)[6] with an intervention order of b=0, s=5, and r=2, with MAPE and MAE values are 4,38% and 0,397.  Forecasting results from December 2021 to June 2022 respectively, are 2550, 756, 347, 515, 1585, 1287 and  2247 people.  The number of visitors will increase during the holiday season, i.e. December and June.Keywords: Intervention model; Londa tourism object; step function
本研究的目的是确定Londa旅游项目中游客数量数据的干预模型,并预测2021年12月至2022年6月的Londa旅游对象数据的干预模式,并使用sepa sepa集成动动型(SARIMA)。所使用的数据是隆达旅游对象公司在2018年1月至2021年11月期间每月出访人数的数据。朗达旅游景点的数据模式在2020年4月(T=28)经历了显著的波动。本研究中使用的干预模式是步函数,因为影响在2020年4月至2021年11月(T=28 - T=47)的一段时间内发生。来自数据分析结果的最佳模型是SARIMA(1.0)(1.1.0)[6],干预顺序b=0、s=5、r=2, MAPE和MAE分别为4.38%和0,397。预测结果分别是2550年、756年、347年、515年、1585年、1287年和2247年。在十二月和六月的假日期间,游客的数量将会增加。关键词:干预模式;step功能;2020年12月21日,我们使用区域可分割的移动平均分配(SARIMA)模型,我们研究的对象、对象和对象的对象的影响影响因素。过去使用的数据从2018年1月到2021年11月,来自龙田tourist对象的经理。2020年4月隆达访客访客数号上的数据集中了大量的重要液体。受委托的干预模式是一个步骤,因为从2020年4月到2021年11月期间发生的影响影响到时间的影响。分析数据结果结果的最佳模型是SARIMA(1.1 0)(1.1 0)[6],具有b项干预令=0、s=5、r=2、MAPE和MAE values为4.38%和0,397。从2021年12月到2022年6月,预测结果为2550、756、347、515、1585、1287和2247人。信众的电话号码将在假日12月6日和6月增加。关键词:模式干预;朗达旅游对象;step功能
{"title":"Penerapan Metode SARIMA dalam Model Intervensi Fungsi Step untuk Memprediksi Jumlah Pegunjung Objek Wisata Londa (Application of the SARIMA Method in the Step Function Intervention to Predict the Number of Visitors at Londa Tourism Object)","authors":"Geovani Christie, Djoni Hatidja, Rinancy Tumilaar","doi":"10.35799/jis.v22i2.40961","DOIUrl":"https://doi.org/10.35799/jis.v22i2.40961","url":null,"abstract":"Tujuan dari penelitian ini yaitu menentukan model intervensi pada data jumlah pengunjung di objek wisata Londa dan memprediksi jumlah pengunjung objek wisata Londa pada bulan Desember 2021 sampai Juni 2022 menggunakan model Seasonal Autoregresive Integrated Moving Average (SARIMA). Data yang digunakan adalah data jumlah pengunjung bulanan pada periode bulan Januari 2018 sampai bulan November 2021, yang diperoleh dari pihak pengelolah objek wisata Londa.  Pola data jumlah pengunjung pada objek wisata Londa mengalami fluktuasi yang signifikan, yaitu pada bulan April 2020 (T=28). Model intervensi yang digunakan dalam penelitian ini yaitu fungsi step karena pengaruh yang terjadi berlangsung dalam kurun waktu cukup lama yaitu pada bulan April 2020 sampai November 2021 (T=28 sampai T=47).  Model terbaik yang diperoleh dari hasil analisis data yaitu SARIMA (1,1,0)(1,1,0)[6] dengan orde intervensi b=0, s=5, dan r=2, dengan nilai MAPE dan MAE masing-masing sebesar 4,38% dan 0,397.  Hasil prediksi dari bulan Desember 2021 sampai Juni 2022 secara berturut-turut yaitu 2550, 756, 347, 515, 1585, 1287 dan 2247 orang.  Jumlah pengunjung akan meningkat pada waktu musim liburan yaitu Bulan Desember dan Juni.Kata kunci: Model intervensi; fungsi step; objek wisata Londa,ABSTRACTThe object of this study are to determine the intervention model of the number of visitors at the Londa tourism object and predict the number of visitors at the Londa tourism object for December 2021 to June 2020 using Seasonal Autoregresive Integrated Moving Average (SARIMA) models. The data used is monthly data for the period from January 2018 to November 2021, which was obtained from the manager of the Londa tourist object. The data pattem on the number of visitors at the Londa tourism object experienced significant fluctuations on April 2020 (T=28). The suspected intervention model is a step function because the effect of the intervention that occurred took place over a long period of time, from April 2020 to November 2021 (T=28 to T=47). The best model obtained from the results of data analysis is SARIMA (1,1,0)(1,1,0)[6] with an intervention order of b=0, s=5, and r=2, with MAPE and MAE values are 4,38% and 0,397.  Forecasting results from December 2021 to June 2022 respectively, are 2550, 756, 347, 515, 1585, 1287 and  2247 people.  The number of visitors will increase during the holiday season, i.e. December and June.Keywords: Intervention model; Londa tourism object; step function","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78396837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pengaruh Konsentrasi Probiotik Terhadap Kepadatan dan Komposisi Infusoria yang Ditumbuhkan pada Substrat Daun Pisang (Musa paradisiaca) 益生菌浓度对香蕉叶下(摩西范例)产生的炎症聚合和炎症成分的影响
Pub Date : 2022-07-24 DOI: 10.35799/jis.v22i2.39785
Laras Puput Insanni, S. Sucahyo, D. Cahyaningrum
Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi probiotik terhadap kepadatan dan komposisi infusoria yang ditumbuhkan pada subtrat daun pisang (Musa paradisiaca). Penelitian ini merupakan penelitian eksperimental Rancangan Acak Lengkap (RAL) satu faktor dengan lima taraf perlakuan yaitu konsentrasi probiotik 0 ml/l, 0,5 ml/l, 1 ml/l, 2 ml/l,  dan 4 ml/l dengan pengulangan sebanyak 4 kali untuk setiap perlakuan.  Pengamatan dilakukan pada hari ke 2, 4, 6, 8, 10, 12, dan 14 untuk memperoleh data berupa kepadatan infusoria, keanekeragaman spesies infusoria beserta jumlah individu per spesies. Data tersebut digunakan untuk mengetahui kepadatan, indeks keanekaragaman (H’), indeks dominansi, serta komposisi spesies infusoria pada setiap perlakuan. Hasil penelitian menunjukkan bahwa konsentrasi probiotik berpengaruh signifikan terhadap kepadatan insufosia (sig 0,010) berdasarkan analisis One Way ANOVA. Indeks keanekaragaman infusoria termasuk dalam kategori rendah pada semua perlakuan. Sejalan dengan hasil tersebut, indeks dominansi pada semua perlakuan berada dalam kategori tinggi. Hasil penelitian menunjukkan bahwa Paramecium sp mendominasi komposisi infusoria pada semua konsentrasi probiotik. Konsentrasi probiotik sebesar 0,5 ml/l,  1 ml/l memicu pertumbuhan Chlorococcum dan digantikan populasi Trichocerca tenuior pada konsentrasi 2 ml/l dan 4 ml/l. Kata kunci: dominansi, infusoria, keanekaragaman, probiotik Probiotic Concentrations Effect on Density and Compotition of Infusoria Grown on Banana Leaf Substrate ABSTRACTThis study aims to determine the effect of probiotic concentration on the density and composition of infusoria grown on banana leaf substrate (Musa paradisiaca). This research is an experimental study in a one-factor Completely Randomized Design (CRD) with five levels of treatment, namely the probiotic concentration 0 ml/l,  0,5 ml/l, 1 ml/l,  2 ml/l and 4 ml/l with 4 repetitions for each treatment. Observations were made on days 2, 4, 6, 8, 10, 12, and 14 to obtain data in the form of infusoria density, infusoria species diversity and the number of individuals per species. These data were used to determine the density, diversity index (H'), dominance index, and the composition of infusoria species in each treatment. The results showed that the concentration of probiotics had a significant effect on the density of insuffocia (sig 0.010) based on One Way ANOVA analysis. The infusoria diversity index was included in the low category in all treatments. In line with these results, the dominance index in all treatments was in the high category. The results showed that Paramecium sp dominated the composition of the infusoria at all concentrations. The probiotic concentration was 0,5ml/l; 1ml/l; stimulated the growth of Chlorococcum and was replaced by Trichocerca tenuior populations at concentrations of 2 ml/l and 4 ml/l.Keywords: dominance, infusoria, diversity, probiotic
这项研究的目的是确定益生菌浓度和炎症组成对香蕉叶下(摩西范例)的影响。这项研究是一项完整的随机设计实验研究(RAL),五种治疗方法是0.5 ml/l、0.5 ml/l、1 ml/l、2 ml/l和4 ml/l,每次治疗重复4次。观察工作进行到第2天、4天、6天、8天、10天、12天和14天,以获取inforia密度、inforia物种多样性和单个物种数量的数据。这些数据用于确定密度、多样性指数、支配率以及每种行为中次热性物种的组成。研究表明,益生菌浓度在一种方法中对胰岛素密度(sig 0.010)有显著影响。红血球多样性指数属于所有行为的次要类别。按照这些结果,所有治疗的优待指数都属于高类别。研究表明,sp Paramecium在所有益生菌浓度中主导了炎症成分。益生菌浓度为0.5毫升/l, 1ml /l促进氯球菌生长,在2毫升/l和4毫升/l的浓度中取代三冠目目动物种群。关键字:支配,infusoria,多样性,关于生殖的益生结果的结果这一研究是一种实验,在五层的试验中进行研究,每层的结果为0.0ml /l, 0.5毫升/l, 1 ml/l, 2 ml/l和4 ml/l重复使用4毫升/l。观察结果是在2天、4天、6天、8天、10日、12日和14日公布在红外线、红外线物种和个别个体的编号上的数据。这些数据被用来确定密度、多样性指数、自治指数和每个领域的炎症因子。结果表明,probiotics的集中影响在于绝对性分析(sig 010)。各种各样的血液指数都包括在低剂量的治疗中。在这些结果的支持下,所有诉讼的合法性都受到了考验。被告在所有的中心都控制着infuia的composition。probiotic浓度是0.5ml /l;100毫升/ l;磷酸葡萄球菌的生长被2毫升/l和4毫升的低降解。支配,耻辱,多样性,新生
{"title":"Pengaruh Konsentrasi Probiotik Terhadap Kepadatan dan Komposisi Infusoria yang Ditumbuhkan pada Substrat Daun Pisang (Musa paradisiaca)","authors":"Laras Puput Insanni, S. Sucahyo, D. Cahyaningrum","doi":"10.35799/jis.v22i2.39785","DOIUrl":"https://doi.org/10.35799/jis.v22i2.39785","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi probiotik terhadap kepadatan dan komposisi infusoria yang ditumbuhkan pada subtrat daun pisang (Musa paradisiaca). Penelitian ini merupakan penelitian eksperimental Rancangan Acak Lengkap (RAL) satu faktor dengan lima taraf perlakuan yaitu konsentrasi probiotik 0 ml/l, 0,5 ml/l, 1 ml/l, 2 ml/l,  dan 4 ml/l dengan pengulangan sebanyak 4 kali untuk setiap perlakuan.  Pengamatan dilakukan pada hari ke 2, 4, 6, 8, 10, 12, dan 14 untuk memperoleh data berupa kepadatan infusoria, keanekeragaman spesies infusoria beserta jumlah individu per spesies. Data tersebut digunakan untuk mengetahui kepadatan, indeks keanekaragaman (H’), indeks dominansi, serta komposisi spesies infusoria pada setiap perlakuan. Hasil penelitian menunjukkan bahwa konsentrasi probiotik berpengaruh signifikan terhadap kepadatan insufosia (sig 0,010) berdasarkan analisis One Way ANOVA. Indeks keanekaragaman infusoria termasuk dalam kategori rendah pada semua perlakuan. Sejalan dengan hasil tersebut, indeks dominansi pada semua perlakuan berada dalam kategori tinggi. Hasil penelitian menunjukkan bahwa Paramecium sp mendominasi komposisi infusoria pada semua konsentrasi probiotik. Konsentrasi probiotik sebesar 0,5 ml/l,  1 ml/l memicu pertumbuhan Chlorococcum dan digantikan populasi Trichocerca tenuior pada konsentrasi 2 ml/l dan 4 ml/l. Kata kunci: dominansi, infusoria, keanekaragaman, probiotik Probiotic Concentrations Effect on Density and Compotition of Infusoria Grown on Banana Leaf Substrate ABSTRACTThis study aims to determine the effect of probiotic concentration on the density and composition of infusoria grown on banana leaf substrate (Musa paradisiaca). This research is an experimental study in a one-factor Completely Randomized Design (CRD) with five levels of treatment, namely the probiotic concentration 0 ml/l,  0,5 ml/l, 1 ml/l,  2 ml/l and 4 ml/l with 4 repetitions for each treatment. Observations were made on days 2, 4, 6, 8, 10, 12, and 14 to obtain data in the form of infusoria density, infusoria species diversity and the number of individuals per species. These data were used to determine the density, diversity index (H'), dominance index, and the composition of infusoria species in each treatment. The results showed that the concentration of probiotics had a significant effect on the density of insuffocia (sig 0.010) based on One Way ANOVA analysis. The infusoria diversity index was included in the low category in all treatments. In line with these results, the dominance index in all treatments was in the high category. The results showed that Paramecium sp dominated the composition of the infusoria at all concentrations. The probiotic concentration was 0,5ml/l; 1ml/l; stimulated the growth of Chlorococcum and was replaced by Trichocerca tenuior populations at concentrations of 2 ml/l and 4 ml/l.Keywords: dominance, infusoria, diversity, probiotic","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91376866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Skrining Fitokimia dan Uji Antibakteri Masker Peel-Off Ekstrak Etanol Daun Kalu Burung (Barleria prionitis L.)
Pub Date : 2022-05-01 DOI: 10.35799/jis.v22i1.38954
Meriam Gratia Gabriel Wowor, Josua Tampara, Edy Suryanto, L. Momuat
Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder yang terdapat pada ekstrak etanol daun kalu burung, membuat masker peel-off berbahan aktif ekstrak etanol daun kalu burung dan menguji aktivitas antibakteri dari ekstrak etanol dan sediaan masker peel-off. Metode yang digunakan adalah preparasi sampel, ekstraksi, uji senyawa fitokimia, pembuatan masker peel-off, uji sifat fisik dan kimia masker meliputi uji organoleptik, stabilitas fisik, lama mengering dan pH, serta uji antibakteri. Hasil penelitian menunjukkan bahwa serbuk daun kalu burung memiliki kadar air sebesar 6.13% dan rendemen ekstrak etanol sebesar 10.34%. Ekstrak etanol daun kalu burung mengandung senyawa metabolit sekunder flavonoid, saponin, tanin, steroid dan alkaloid pada uji Dragendorff. Sediaan masker peel-off memiliki karakteristik fisik dan kimia yang aman digunakan pada kulit. Masker peel-off memiliki kisaran pH antara 5-6 dan lama mengering dengan kisaran antara 23-29 menit. Zona hambat pertumbuhan bakteri untuk ekstrak etanol daun kalu burung sebesar 12.33 mm dan masker peel-off mengandung daun kalu burung sebesar 9.67 mm. Penelitian ini menyimpulkan bahwa daun kalu burung mengandung metabolit sekunder yang memiliki aktivitas antibakteri baik dalam ekstrak etanolnya maupun dalam sediaan masker peel-off ekstrak dan mempunyai sifat fisik dan kimia yang aman.Kata kunci: antibakteri; fitokimia; kalu burung; masker peel-off Phytochemical Screening and Antibacterial Test of Peel-Off Mask with Ethanol Extracts of Kalu Burung Leaves (Barleria prionitis L.).ABSTRACTThis research aims to determine the content of secondary metabolites contained in the ethanol extract of Kalu Burung leaves, to make peel-off masks from ethanol extract of Kalu Burung leaves and to test the antibacterial activity of ethanol extracts and peel-off masks. The methods used were sample preparation, extraction, testing of phytochemical compounds, making peel-off masks, testing the physical and chemical properties of masks such as organoleptic tests, physical stability, drying time and pH, as well as antibacterial tests. The results showed that the Kalu Burung leaf powder had a moisture content of 6.13% and an ethanol extract yield of 10.34%. The ethanol extract of Kalu Burung leaves contains secondary metabolites of flavonoids, saponins, tannins, steroids and alkaloids in the Dragendorff test. The peel-off mask has physical and chemical characteristics which are safe to use. Peel-off masks have a pH range of 5-6 and drying time of 23-29 minutes. The inhibition zone of bacterial growth for ethanol extract was 12.33 mm and the peel-off mask containing Kalu Burung leaves was 9.67 mm. In conclusion, Kalu Burung leaves contain secondary metabolites that have antibacterial activity both in the ethanol extract and in the peel-off extract mask with safe physical and chemical properties.Keywords: antibacterial; kalu burung leaves; peel-off mask; phytochemistry
这项研究的目的是确定鸟的叶子乙醇提取物中存在的次生代谢物质,使鸟的叶子乙醇提取物活性,并测试从乙醇提取物和peal -off面罩提取物的抗菌活性。使用的方法包括对样本进行筛选、提取、化合物测试、对面膜的制造、对面膜的物理性质和化学性质进行检测,包括有机检测、物理稳定性、长期干燥和pH测试以及抗菌检测。研究表明,真菌叶粉的含水率为6.13%,乙醇浸渍为10.34%。这种鸟的乙醇提取物中含有一种由类黄酮、髓磷脂、单宁、类固醇和生物碱组成的副代谢化合物。peel-off面料具有在皮肤上安全使用的物理和化学特征。peel-off面膜的pH值在5-6到23-29分钟之间。该区域抑制了鸟类以12.33毫米(0.6英寸)为原料提取乙醇的细菌生长,而切毛面膜中含有9.67毫米(2.67毫米)的叶鞘。这项研究得出结论,木质素的叶子含有次生代谢物,在其乙醇提取物和果皮面罩中都有抗菌活性,具有安全的生理和化学性质。关键词:抗菌;植物化学;如果鸟;面膜脱落的植物化学筛子和抑制抑制抑制面膜的果肉乙醇。根据这项研究,这项研究旨在确定在乙醇中包裹的二代谢产物的含量,从乙醇extract中去除颗粒,测试乙醇萃取和果皮的抗菌活性酶。过去的治疗方法是样本准备,提取,实验植物化学化合物,脱去马甲,测试草药和化学性能,测试微生物测试、物理稳定、干燥时间和pH值,如抗菌测试。结果表明,叶波德特有6.13%的水分和10.34%的乙醇排泄物。在Dragendorff测试中,乙醇extract of The ethanol extract of flavonoids, saponins, tannins, steroids and alkaloids in Dragendorff test。面膜上的果皮具有生理和化学特征,可以使用。果皮披萨的pH值为5-6,干燥时间为23-29分钟。对乙醇出口的细菌生长抑制是12.33毫米,而脱皮面罩接触表明鸟类为9.67毫米。在conclusion中,如果这只鸟的新陈代谢完全不同,它会在乙醇出洋点和喙面与安全的物理和化学性能进行代谢。安装:antibacterial;卡鲁鸟离开;peel-off面具;phytochemistry
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引用次数: 1
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JURNAL ILMIAH SAINS
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