Pub Date : 2023-03-31DOI: 10.26740/jrba.v5n1.p8-15
S. M. Mahmood, Adnan Musa Mohammed
Earthworms are key to the earth's ecosystem, which helps the soil increase its fertility and repair its existing elements, as well as remove contaminants. This study investigated the accumulation of Zn in the earthworm Aporrectodea caliginosa after 15 days of exposure. The worms were grown in media with different concentrations of Zn between 750 and 1500 ppm. Each treatment consisted of three replicates, containing 30 worms. A control group without Zn was also used. Data were analyzed by using Duncan Multi–Range. The results revealed that A. caliginosa had a strong ability to accumulate Zn in its tissue compared to the control group. It was noticed that the increase of the heavy metal in the worm´s tissue is associated with the elevation of the metal in its media. The results show a significant loss of weight in the worm´s body and loss in the growth rate; they also shown a decrease in specific growth rate. Moreover, there was a significant decrease in the worm length, showing a high effect, especially after 15 days of breeding with all concentrations used in this study. The researchers recommend using earthworms to purify the soil from contaminants because earthworm has a great ability to get rid of all pollutants, whether metals or pesticides or parasites, especially in industrialized countries and agricultural lands.
{"title":"Effects of Zinc Accumulation on Earthworm Aporrectodea caliginosa (Haplotaxida: Lumbricidae)","authors":"S. M. Mahmood, Adnan Musa Mohammed","doi":"10.26740/jrba.v5n1.p8-15","DOIUrl":"https://doi.org/10.26740/jrba.v5n1.p8-15","url":null,"abstract":"Earthworms are key to the earth's ecosystem, which helps the soil increase its fertility and repair its existing elements, as well as remove contaminants. This study investigated the accumulation of Zn in the earthworm Aporrectodea caliginosa after 15 days of exposure. The worms were grown in media with different concentrations of Zn between 750 and 1500 ppm. Each treatment consisted of three replicates, containing 30 worms. A control group without Zn was also used. Data were analyzed by using Duncan Multi–Range. The results revealed that A. caliginosa had a strong ability to accumulate Zn in its tissue compared to the control group. It was noticed that the increase of the heavy metal in the worm´s tissue is associated with the elevation of the metal in its media. The results show a significant loss of weight in the worm´s body and loss in the growth rate; they also shown a decrease in specific growth rate. Moreover, there was a significant decrease in the worm length, showing a high effect, especially after 15 days of breeding with all concentrations used in this study. The researchers recommend using earthworms to purify the soil from contaminants because earthworm has a great ability to get rid of all pollutants, whether metals or pesticides or parasites, especially in industrialized countries and agricultural lands.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77467002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.26740/jrba.v5n1.p27-36
Reeno Al Hikmatus Sholekah, D. Listyorini
Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.
{"title":"Genetic Identification of Superbugs from River Streams in State University of Malang","authors":"Reeno Al Hikmatus Sholekah, D. Listyorini","doi":"10.26740/jrba.v5n1.p27-36","DOIUrl":"https://doi.org/10.26740/jrba.v5n1.p27-36","url":null,"abstract":"Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88983935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.26740/jrba.v4n2.p83-89
D. Winarni, Lita Lestari, A. Hayati, Nithasya Nabilla, F. Khaleyla
Acaudina rosettis is one of the commercial sea cucumber species that is widely consumed on the east coast of Surabaya, East Java, Indonesia. Although its abundance in nature has begun to show signs of decreasing, studies on this species are still very limited. This study aimed to examine indicators that can be used to determine the sex of A. rosettis individuals. Acaudina rosettis samples were collected from Madura strait in the mid of Hijri month for three months. The sea cucumber samples were then dissected. Body wall without viscera and gonads was weighed. The gonads were then characterized macroscopically and microscopically, prepared into histological slides with hematoxylin-eosin staining. The results showed that A. rosettis samples could be classified into three sex groups based on the type of gamete cells observed in the tubules; male (spermatogenic), female (oogenic), and undetermined sex (no gamete cells found). Gonads had three colors; yellow, pink, and orange and were in five stages of reproductive phase; gametogenesis (G), mature (M), spawning (S), and post-spawning (PS). Male samples had yellow and orange gonads, female samples were pink and orange, and all undetermined sex were orange. The G gonads were yellow and orange, M were yellow and pink, while S and PS were orange. Body wall and gonad weight at the same reproductive phase did not show any difference between males and females. Based on the results, gonad color can be used as an indicator of sex determination, but only in G and M reproductive phases.
{"title":"Sex Determination of Sea Cucumber Acaudina rosettis from Madura Straits, Indonesia","authors":"D. Winarni, Lita Lestari, A. Hayati, Nithasya Nabilla, F. Khaleyla","doi":"10.26740/jrba.v4n2.p83-89","DOIUrl":"https://doi.org/10.26740/jrba.v4n2.p83-89","url":null,"abstract":"Acaudina rosettis is one of the commercial sea cucumber species that is widely consumed on the east coast of Surabaya, East Java, Indonesia. Although its abundance in nature has begun to show signs of decreasing, studies on this species are still very limited. This study aimed to examine indicators that can be used to determine the sex of A. rosettis individuals. Acaudina rosettis samples were collected from Madura strait in the mid of Hijri month for three months. The sea cucumber samples were then dissected. Body wall without viscera and gonads was weighed. The gonads were then characterized macroscopically and microscopically, prepared into histological slides with hematoxylin-eosin staining. The results showed that A. rosettis samples could be classified into three sex groups based on the type of gamete cells observed in the tubules; male (spermatogenic), female (oogenic), and undetermined sex (no gamete cells found). Gonads had three colors; yellow, pink, and orange and were in five stages of reproductive phase; gametogenesis (G), mature (M), spawning (S), and post-spawning (PS). Male samples had yellow and orange gonads, female samples were pink and orange, and all undetermined sex were orange. The G gonads were yellow and orange, M were yellow and pink, while S and PS were orange. Body wall and gonad weight at the same reproductive phase did not show any difference between males and females. Based on the results, gonad color can be used as an indicator of sex determination, but only in G and M reproductive phases.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77340053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.26740/jrba.v4n2.p69-74
Risqi Aprilianingsih, Baiq Farhatul Wahidah, Muhammad Rifqi Hariri
Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species.
{"title":"DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region","authors":"Risqi Aprilianingsih, Baiq Farhatul Wahidah, Muhammad Rifqi Hariri","doi":"10.26740/jrba.v4n2.p69-74","DOIUrl":"https://doi.org/10.26740/jrba.v4n2.p69-74","url":null,"abstract":"Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78059996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.26740/jrba.v4n2.p90-95
N. Indah, Ashari Bagus Setiawan
Tarenna is a genus of relatives of Ixora which is found in forests. Tarenna is different from Ixora which is known as an ornamental plant. The purpose of this study was to find the genus Tarenna in Madura natural habitat to preserved or conserved before this genus became extinct. Observations were made on morphological characters. The morphological characteristics observed included: stature, stems, leaves, inflorescences, flowers, fruits, and seeds. Ixora has a corolla of various flowers while Tarenna only has a white corolla but smells good. Recent exploration and collection of Tarenna in Madura Island indicated that two species are a new record for Java and Madura Island, namely T. costata and T. fragrans. Existence of T. costata in this area not only as a new record for Madura Island, but also as a new record for Java. Tarenna fragrans was firstly reported in Madura Island as a new distributional record for this area. An updated of identification key, several descriptions, and documentations, as well as a distributional map are provided.
{"title":"An Additional Information of Tarenna (Rubiaceae) in Madura Island","authors":"N. Indah, Ashari Bagus Setiawan","doi":"10.26740/jrba.v4n2.p90-95","DOIUrl":"https://doi.org/10.26740/jrba.v4n2.p90-95","url":null,"abstract":"Tarenna is a genus of relatives of Ixora which is found in forests. Tarenna is different from Ixora which is known as an ornamental plant. The purpose of this study was to find the genus Tarenna in Madura natural habitat to preserved or conserved before this genus became extinct. Observations were made on morphological characters. The morphological characteristics observed included: stature, stems, leaves, inflorescences, flowers, fruits, and seeds. Ixora has a corolla of various flowers while Tarenna only has a white corolla but smells good. Recent exploration and collection of Tarenna in Madura Island indicated that two species are a new record for Java and Madura Island, namely T. costata and T. fragrans. Existence of T. costata in this area not only as a new record for Madura Island, but also as a new record for Java. Tarenna fragrans was firstly reported in Madura Island as a new distributional record for this area. An updated of identification key, several descriptions, and documentations, as well as a distributional map are provided.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89594368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.26740/jrba.v4n2.p75-82
Nadhifah Raniah, Henri Henri, Kurniawan Kurniawan
The mangrove ecosystem is one of the most productive ecosystems on earth. This ecosystem provides a lot of functions for the living organism inhabiting here, such as fishes, mollusks, echinoderms, crustaceans, ect. The mangrove crab is one of the common crustaceans that can be found in the mangrove. This Purtunidae crab is very popular due to their role in the food chain as well as for the economic income that has a high value in the market. However, overexploitation may decrease the crab population. This study aimed to investigate the abundance and occurrence of the mangrove crabs from Munjang mangrove, Bangka Belitung Island from August 2020 to June 2021 using baited traps with the line transect method at four zones. A total of four species of mangrove crabs, Scylla olivacea, S. paramamosain, S. serrata and S. tranquebarica, were found in this location and dominated by S. serrata. Shannon diversity index (H’) was a in low category (<1), evenness index range (J) from 0.35 to 0.89, and there was dominant species in this study. The bed sediment iswas dominated by the muddy clay substratum except in zone 4 with sandy. Overall, this location is a suitable habitat for the Scylla spp. And better management is urgently needed before these crabs are exploited by humans.
{"title":"On the abundance and occurrence of the mangrove crabs, Scylla spp. (Crustacea: Portunidae) from Munjang mangrove, Bangka Belitung Island","authors":"Nadhifah Raniah, Henri Henri, Kurniawan Kurniawan","doi":"10.26740/jrba.v4n2.p75-82","DOIUrl":"https://doi.org/10.26740/jrba.v4n2.p75-82","url":null,"abstract":"The mangrove ecosystem is one of the most productive ecosystems on earth. This ecosystem provides a lot of functions for the living organism inhabiting here, such as fishes, mollusks, echinoderms, crustaceans, ect. The mangrove crab is one of the common crustaceans that can be found in the mangrove. This Purtunidae crab is very popular due to their role in the food chain as well as for the economic income that has a high value in the market. However, overexploitation may decrease the crab population. This study aimed to investigate the abundance and occurrence of the mangrove crabs from Munjang mangrove, Bangka Belitung Island from August 2020 to June 2021 using baited traps with the line transect method at four zones. A total of four species of mangrove crabs, Scylla olivacea, S. paramamosain, S. serrata and S. tranquebarica, were found in this location and dominated by S. serrata. Shannon diversity index (H’) was a in low category (<1), evenness index range (J) from 0.35 to 0.89, and there was dominant species in this study. The bed sediment iswas dominated by the muddy clay substratum except in zone 4 with sandy. Overall, this location is a suitable habitat for the Scylla spp. And better management is urgently needed before these crabs are exploited by humans.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79732735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.26740/jrba.v4n2.p51-56
Herin Yoga Lesti, Miftahul Ilmi
Oleaginous fungi are one of the microorganisms that can accumulate a high number of biomasses quickly (within 96-130 hours) and are often used to produce lipids. The growth of fungi depends on the chemical composition of the environment in which it grows. The growth media of fungi must contain high carbohydrates as a source of nutrients and high nitrogen content. One of the carbon sources that fungi can use in the growth process is glucose. BR 2.2 isolate is an oleaginous fungus capable of accumulating 28.44% lipids from the total dry biomass with glucose as a carbon source in 50 mL of growth media. Therefore, this study was conducted to determine the effect of variations in the volume of media and incubation time on the production of biomass and lipid isolate BR 2.2. Biomass and lipid production were analyzed at media with additional water volumes of 10, 20, 30, 40, and 50 mL with 48, 96, and 144 hours of incubation times. The results showed that lipid accumulation and biomass production increased with the reduction of water content in the growth media and reached the highest number in the media volume of 20 mL with an incubation time of 144 hours, i.e., 0.87±0.04 g/L and 12.53±0.29 g/L. It can be concluded that biomass and fungal lipid increased along with incubation time and nutrient concentration.
{"title":"The Effect of Water Concentration on Growth Media on Lipid Production by Oleaginous Fungi Isolate BR 2.2","authors":"Herin Yoga Lesti, Miftahul Ilmi","doi":"10.26740/jrba.v4n2.p51-56","DOIUrl":"https://doi.org/10.26740/jrba.v4n2.p51-56","url":null,"abstract":"Oleaginous fungi are one of the microorganisms that can accumulate a high number of biomasses quickly (within 96-130 hours) and are often used to produce lipids. The growth of fungi depends on the chemical composition of the environment in which it grows. The growth media of fungi must contain high carbohydrates as a source of nutrients and high nitrogen content. One of the carbon sources that fungi can use in the growth process is glucose. BR 2.2 isolate is an oleaginous fungus capable of accumulating 28.44% lipids from the total dry biomass with glucose as a carbon source in 50 mL of growth media. Therefore, this study was conducted to determine the effect of variations in the volume of media and incubation time on the production of biomass and lipid isolate BR 2.2. Biomass and lipid production were analyzed at media with additional water volumes of 10, 20, 30, 40, and 50 mL with 48, 96, and 144 hours of incubation times. The results showed that lipid accumulation and biomass production increased with the reduction of water content in the growth media and reached the highest number in the media volume of 20 mL with an incubation time of 144 hours, i.e., 0.87±0.04 g/L and 12.53±0.29 g/L. It can be concluded that biomass and fungal lipid increased along with incubation time and nutrient concentration.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82574431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.26740/jrba.v4n2.p57-68
Afifah Nuraini, Wisanti Wisanti
Filicinae is a fern with the most species members. The Filicinae includes approximately 170 genera and 7000 species. Filicinae grows in wet, humid, and shady environmental conditions, like waterfall areas with an altitude of 1100-1300 meters above sea level (m a s l). The study aimed to determine the taxonomic diversity of the Filicinae in the Tretes Wonosalam Waterfall Tourism area. Taxonomic diversity is very important in tourism areas because it prevents species extinction due to the destruction of the habitat, they live in. This was descriptive research with exploration and observation techniques used purposive sampling methods based on altitude. The results of the study were stated in the form of an inventory list of species and families of each station. Data gained of species diversity were analyzed using taxonomic diversity with the formula (∆) and dominance diversity with the formula (∆*). Exploration results found 37 species from 12 families composed of 10 species from 7 families at station 1; 12 species from 6 families at station 2; 14 species from 7 families at station 3; and 22 species from 12 families at station 4. The highest taxonomic diversity (∆) was station 4 with 106.8 while the lowest for station 1 was 25.63. Dominance diversity (∆*) at all stations in this study was in the medium category. The highest family found in the study was Tectariaceae with six species.
凤尾蕨是蕨类植物中种类最多的一种。菲律宾科包括约170属和7000种。Filicinae生长在海拔1100-1300米(m as l)的瀑布区等潮湿、潮湿和阴凉的环境条件下。本研究旨在确定Tretes Wonosalam瀑布旅游区Filicinae的分类多样性。分类多样性在旅游区是非常重要的，因为它可以防止物种因栖息地的破坏而灭绝。这是一项描述性研究，使用基于海拔的有目的采样方法进行勘探和观察技术。研究结果以每个站点的物种和科的清单的形式陈述。物种多样性数据采用分类多样性公式(∆)和优势多样性公式(∆*)进行分析。探测结果发现12科37种，其中1站7科10种;站2 6科12种;3号站7科14种;4号站有12科22个物种。4号站的分类多样性(∆)最高，为106.8,1号站最低，为25.63。本研究各站点优势多样性(∆*)均为中等。在本研究中发现的最高科是六种的龙舌兰科。
{"title":"Filicinae Taxonomic Diversity in the Tourism Area of Tretes Waterfall Wonosalam Kabupaten Jombang","authors":"Afifah Nuraini, Wisanti Wisanti","doi":"10.26740/jrba.v4n2.p57-68","DOIUrl":"https://doi.org/10.26740/jrba.v4n2.p57-68","url":null,"abstract":"Filicinae is a fern with the most species members. The Filicinae includes approximately 170 genera and 7000 species. Filicinae grows in wet, humid, and shady environmental conditions, like waterfall areas with an altitude of 1100-1300 meters above sea level (m a s l). The study aimed to determine the taxonomic diversity of the Filicinae in the Tretes Wonosalam Waterfall Tourism area. Taxonomic diversity is very important in tourism areas because it prevents species extinction due to the destruction of the habitat, they live in. This was descriptive research with exploration and observation techniques used purposive sampling methods based on altitude. The results of the study were stated in the form of an inventory list of species and families of each station. Data gained of species diversity were analyzed using taxonomic diversity with the formula (∆) and dominance diversity with the formula (∆*). Exploration results found 37 species from 12 families composed of 10 species from 7 families at station 1; 12 species from 6 families at station 2; 14 species from 7 families at station 3; and 22 species from 12 families at station 4. The highest taxonomic diversity (∆) was station 4 with 106.8 while the lowest for station 1 was 25.63. Dominance diversity (∆*) at all stations in this study was in the medium category. The highest family found in the study was Tectariaceae with six species.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89854852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vilya Syafriana, S. Nuswantara, W. Mangunwardoyo, P. Lisdiyanti
In the previous study, Penicillium sp. ID10-T065 has been mutated using ultraviolet (UV), ethyl methyl sulfonate (EMS) and the combination of UV-EMS to increase Beta-glucosidase (bgl) activity. There were three mutants selected, UV13 (UV mutant), EM31 (EMS mutant), and UM23 (UV-EMS mutant). This study examined the mutations in the bgl gene encoding (bgl1) as well as sequence differences between mutants and wild-type of Penicillium sp. ID10-T065. The gene analysis was performed by PCR amplification and sequencing of the bgl1 gene. The results of bgl1 gene sequences (600 bp) from mutants were aligned with the wild-type, it was discovered that there were base alterations from position 2025 to 2050. Mutant UV13 showed the highest base alterations (7 bases) which occurred at position 2027 (T→C); 2036 (T→G); 2040 (T→G); 2047 (G→C); and 2048-2050 (TTG→GGA). Mutant EM31 showed alterations in five bases at positions 2034 (G→A), 2036 (T→G), 2037 (G→C), 2044 (G→C), and 2047 (G→T). Mutant UM23 showed two base alterations at position 2025 (T→A) and 2037 (G→C). UV irradiation and EMS mutation resulted in transition and transversion DNA, whereas the combination of UV-EMS mutation resulted in transversion mutations. Base alterations in UV13 and EM31 mutants, causing missense and silent mutation, while in UM23 mutant only silent mutations occur. The bgl1 gene analysis showed that mutation using UV light was more effective than using EMS or a combination of UV-EMS in Penicillium sp. ID10-T065.
{"title":"Beta-Glucosidase 1 (bgl1) Gene Analysis in Mutant and Wild-type of Penicillium sp. ID10-T065","authors":"Vilya Syafriana, S. Nuswantara, W. Mangunwardoyo, P. Lisdiyanti","doi":"10.26740/jrba.v4n1.p1-8","DOIUrl":"https://doi.org/10.26740/jrba.v4n1.p1-8","url":null,"abstract":"In the previous study, Penicillium sp. ID10-T065 has been mutated using ultraviolet (UV), ethyl methyl sulfonate (EMS) and the combination of UV-EMS to increase Beta-glucosidase (bgl) activity. There were three mutants selected, UV13 (UV mutant), EM31 (EMS mutant), and UM23 (UV-EMS mutant). This study examined the mutations in the bgl gene encoding (bgl1) as well as sequence differences between mutants and wild-type of Penicillium sp. ID10-T065. The gene analysis was performed by PCR amplification and sequencing of the bgl1 gene. The results of bgl1 gene sequences (600 bp) from mutants were aligned with the wild-type, it was discovered that there were base alterations from position 2025 to 2050. Mutant UV13 showed the highest base alterations (7 bases) which occurred at position 2027 (T→C); 2036 (T→G); 2040 (T→G); 2047 (G→C); and 2048-2050 (TTG→GGA). Mutant EM31 showed alterations in five bases at positions 2034 (G→A), 2036 (T→G), 2037 (G→C), 2044 (G→C), and 2047 (G→T). Mutant UM23 showed two base alterations at position 2025 (T→A) and 2037 (G→C). UV irradiation and EMS mutation resulted in transition and transversion DNA, whereas the combination of UV-EMS mutation resulted in transversion mutations. Base alterations in UV13 and EM31 mutants, causing missense and silent mutation, while in UM23 mutant only silent mutations occur. The bgl1 gene analysis showed that mutation using UV light was more effective than using EMS or a combination of UV-EMS in Penicillium sp. ID10-T065.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"105 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76134147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-31DOI: 10.26740/jrba.v4n1.p9-18
R. K. Asharo, A. Novitasari, S. Azizah, Rahadian Ajeng Saraswati, Fani Setyaningsih, Puput Apriliani, R. Priambodo, Pinta Omas Pasaribu, V. Rizkawati, Usman Usman
Bogor Botanical Gardens is a conservation area that assists the preservation of flora in Indonesia, including the Araceae. Araceae is often used by the public as medicine, food sources, and ornamental plants. Therefore, the Araceae is often used as an interesting research object and conservation efforts have commenced maintaining its sustainability. The purpose of this research was to determine the species of living Araceae and the potential possessed by each living Araceae species in the conservation area of the Bogor Botanical Gardens. This research was conducted on 8-9 June 2021 to determine the diversity and potential of the Araceae in Bogor Botanical Gardens. The method used in this research is the exploring method and data analysis using descriptive method. The result of this research revealed there were 60 species of Araceae consisting of 25 genera. The genera with the highest number of species are Philodendron. Two species of them are Araceae which has a habitat in the waters. A total of 33 species are terrestrial and 25 are epiphytic plants. Araceae have many potentials, such as food, aromatic, medicine, flavoring, animal feed, and ornamental plants. More than 50% of the Araceae species were used as ornamental plants.
{"title":"Araceae Floristic and Potential Study in Bogor Botanical Gardens, West Java, Indonesia","authors":"R. K. Asharo, A. Novitasari, S. Azizah, Rahadian Ajeng Saraswati, Fani Setyaningsih, Puput Apriliani, R. Priambodo, Pinta Omas Pasaribu, V. Rizkawati, Usman Usman","doi":"10.26740/jrba.v4n1.p9-18","DOIUrl":"https://doi.org/10.26740/jrba.v4n1.p9-18","url":null,"abstract":"Bogor Botanical Gardens is a conservation area that assists the preservation of flora in Indonesia, including the Araceae. Araceae is often used by the public as medicine, food sources, and ornamental plants. Therefore, the Araceae is often used as an interesting research object and conservation efforts have commenced maintaining its sustainability. The purpose of this research was to determine the species of living Araceae and the potential possessed by each living Araceae species in the conservation area of the Bogor Botanical Gardens. This research was conducted on 8-9 June 2021 to determine the diversity and potential of the Araceae in Bogor Botanical Gardens. The method used in this research is the exploring method and data analysis using descriptive method. The result of this research revealed there were 60 species of Araceae consisting of 25 genera. The genera with the highest number of species are Philodendron. Two species of them are Araceae which has a habitat in the waters. A total of 33 species are terrestrial and 25 are epiphytic plants. Araceae have many potentials, such as food, aromatic, medicine, flavoring, animal feed, and ornamental plants. More than 50% of the Araceae species were used as ornamental plants.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90632368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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