Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36452
Marsitasari Tandiongan, R. Bara
Secondary metabolites produced by marine organisms commonly have unique chemical structures and very interesting pharmacological activities. Marine organisms that have the most chemical conent are produced by marine invertebrates, followed by marine plants. Groups that include marine invertebrates include: sponges, bryozoans, cnidarians and tunicates. Soft coral, which is one of the marine invertebrates, has biological activities, such as antifungal, cytotoxic, antineoplastic, antimicrobial, HIV inhibitor and anti-inflammatory. This study aims to determine the value of the soft coral extract Cespitularia sp. taken from Manado Bay by the Brine Shrimp Lethality Assay (BSLA) method. This toxicity test was carried out quantitatively by analyzing data based on the number of deaths caused by the test extract of Artemia sp. The results obtained based on data analysis namely the value of the soft coral extract Cespitularia sp. is 53.27 ppm, this means that the extract can be developed as an anticancer drug candidate due to below below to 1000 ppm. Soft coral Cespitularia sp. categorized as toxic based on the Meyer classification or moderately toxic based on the Tanamatayarat classification and very toxic based on the Clarkson classification.Keywords: Soft coral; Cespitularia sp.; Toxicity;
{"title":"TOKSISITAS EKSTRAK KARANG LUNAK Cespitularia sp. DARI TELUK MANADO PROVINSI SULAWESI UTARA","authors":"Marsitasari Tandiongan, R. Bara","doi":"10.35800/jplt.9.3.2021.36452","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36452","url":null,"abstract":"Secondary metabolites produced by marine organisms commonly have unique chemical structures and very interesting pharmacological activities. Marine organisms that have the most chemical conent are produced by marine invertebrates, followed by marine plants. Groups that include marine invertebrates include: sponges, bryozoans, cnidarians and tunicates. Soft coral, which is one of the marine invertebrates, has biological activities, such as antifungal, cytotoxic, antineoplastic, antimicrobial, HIV inhibitor and anti-inflammatory. This study aims to determine the value of the soft coral extract Cespitularia sp. taken from Manado Bay by the Brine Shrimp Lethality Assay (BSLA) method. This toxicity test was carried out quantitatively by analyzing data based on the number of deaths caused by the test extract of Artemia sp. The results obtained based on data analysis namely the value of the soft coral extract Cespitularia sp. is 53.27 ppm, this means that the extract can be developed as an anticancer drug candidate due to below below to 1000 ppm. Soft coral Cespitularia sp. categorized as toxic based on the Meyer classification or moderately toxic based on the Tanamatayarat classification and very toxic based on the Clarkson classification.Keywords: Soft coral; Cespitularia sp.; Toxicity;","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84450735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36679
Vesiy NM Tamburian, Deiske A. Sumilat, C. A. Sinjal
Bioactive compounds are toxic compounds produced by organisms for use in self-defense from a wide variety of predators residing in their environment. The aim of the study was to test antibacterial activity and prove the UV-preventing effectiveness of the AFBK 5c mushroom isolate ethyl acetate extract that is in conjunction with ascidia. This research was conducted for 1 month in two locations, namely for antibacterial testing conducted in the Laboratory of Molecular Biology and Marine Pharmaceuticals, FPIK and for anti-UV testing conducted at the Pharmaceutical Laboratory, FMIPA, UNSRAT. The results showed that; This type of fungus that has been extracted and used is a fungus with the isolate code AFBK 5c This mushroom is a fungus that is connected with sigilina sp type ascidia. The rough extract of ascidia symbiont mushrooms is obtained from the process of meseration using ethyl acetate, then antibacterial testing of S. aureus and E. coli test bacteria and showing results such as antibacterial activity with an average value in S. aureus 09.00 and E. coli 11.05 mm which shows that there is antibacterial activity of AFBK 5c fungus isolate against E. coli and S. aureus test bacteria so that it can be said that the symbiont fungus has the potential as an ingredient. manufacture of antibacterial drugs. Furthermore, anti-UV testing, and the results of testing anti-UV compounds using rough extracts of AFBK 5c fungus isolates can produce absorption in UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm) so that it has the potential to be used as an anti-UV material.Keywords: Bioactive Compound, Ascidia, Meceration, Antibacterial, Anti-UV
{"title":"UJI AKTIVITAS ANTIBAKTERI DAN ANTI-UV DARI EKSTRAK ETIL ASETAT ISOLAT JAMUR AFBK 5c YANG BERSIMBION DENGAN ASCIDIA Sigilina sp. DARI PERAIRAN PULAU BANGKA","authors":"Vesiy NM Tamburian, Deiske A. Sumilat, C. A. Sinjal","doi":"10.35800/jplt.9.3.2021.36679","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36679","url":null,"abstract":"Bioactive compounds are toxic compounds produced by organisms for use in self-defense from a wide variety of predators residing in their environment. The aim of the study was to test antibacterial activity and prove the UV-preventing effectiveness of the AFBK 5c mushroom isolate ethyl acetate extract that is in conjunction with ascidia. This research was conducted for 1 month in two locations, namely for antibacterial testing conducted in the Laboratory of Molecular Biology and Marine Pharmaceuticals, FPIK and for anti-UV testing conducted at the Pharmaceutical Laboratory, FMIPA, UNSRAT. The results showed that; This type of fungus that has been extracted and used is a fungus with the isolate code AFBK 5c This mushroom is a fungus that is connected with sigilina sp type ascidia. The rough extract of ascidia symbiont mushrooms is obtained from the process of meseration using ethyl acetate, then antibacterial testing of S. aureus and E. coli test bacteria and showing results such as antibacterial activity with an average value in S. aureus 09.00 and E. coli 11.05 mm which shows that there is antibacterial activity of AFBK 5c fungus isolate against E. coli and S. aureus test bacteria so that it can be said that the symbiont fungus has the potential as an ingredient. manufacture of antibacterial drugs. Furthermore, anti-UV testing, and the results of testing anti-UV compounds using rough extracts of AFBK 5c fungus isolates can produce absorption in UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm) so that it has the potential to be used as an anti-UV material.Keywords: Bioactive Compound, Ascidia, Meceration, Antibacterial, Anti-UV","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87896102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36091
Priskila S Kodoati, C. Paruntu
The aim of this study was to know the nematocysts composition of Montipora undata (Scleractinia) which was collected in the Malalayang coastal coral reef, Manado Bay. This research was conducted for three months, from October to December 2020. Observation of nematocysts was done under an Olympus CX41 microscope with a magnification of x100 objective lens connected to a computer equipped with the application of Optica view 7 at the Laboratory of Fish Health, Environment and Toxicology, Faculty of Fisheries and Marine Sciences, UNSRAT. The results showed that M. undata had the main nematocyst composition, namely microbasic b-mastigophore (MbM) of 60.0 %, small microbasic p-mastigophore type II (sMpM-II) 26.67 %, small holotrichous isorhiza (sHI) 6 ,67 %, large holotrichous isorhiza (lHI) 4.44 %, and large microbasic p-mastigophore type II (lMpM-II) 2.22 %. MbM is the dominant type of nematocyst for this coral species. Further studies are suggested to investigate the function of MbM and morphology of nematocysts of various types of corals in the genus Montipora.Keywords: Composition, Manado Bay, Montipora undata, nematocyst, Scleractinia
{"title":"NEMATOSIT KARANG Montipora undata (SCLERACTINIA) DARI PANTAI MALALAYANG TELUK MANADO","authors":"Priskila S Kodoati, C. Paruntu","doi":"10.35800/jplt.9.3.2021.36091","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36091","url":null,"abstract":"The aim of this study was to know the nematocysts composition of Montipora undata (Scleractinia) which was collected in the Malalayang coastal coral reef, Manado Bay. This research was conducted for three months, from October to December 2020. Observation of nematocysts was done under an Olympus CX41 microscope with a magnification of x100 objective lens connected to a computer equipped with the application of Optica view 7 at the Laboratory of Fish Health, Environment and Toxicology, Faculty of Fisheries and Marine Sciences, UNSRAT. The results showed that M. undata had the main nematocyst composition, namely microbasic b-mastigophore (MbM) of 60.0 %, small microbasic p-mastigophore type II (sMpM-II) 26.67 %, small holotrichous isorhiza (sHI) 6 ,67 %, large holotrichous isorhiza (lHI) 4.44 %, and large microbasic p-mastigophore type II (lMpM-II) 2.22 %. MbM is the dominant type of nematocyst for this coral species. Further studies are suggested to investigate the function of MbM and morphology of nematocysts of various types of corals in the genus Montipora.Keywords: Composition, Manado Bay, Montipora undata, nematocyst, Scleractinia","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84172262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36067
Ajrin Y Nusi, C. Paruntu, A. Rumengan, Farnis B. Boneka, N. Gustaf F. Mamangkey, L. T. X. Lalamentik
The objective of this present study is to know the composition of nematocyst types from the coral Scleractinia, Pocillopora sp. The coral colonies of Pocillopora sp. were collected at Tontayuo coastal coral reefs, Batudaa Pantai, Gorontalo. This research was conducted for 3 months, from September-December 2020. The method of testing the type and composition of nematocysts was carried out using an Olympus CX41 type microscope with x100 objective lens magnification connected to a computer ST24 monitor equipped with optical view 7 application (software). Photos of Pocillopora sp. both alive and skeleton form are documented in this journal. The results of the current study showed that Pocillopora sp. has the types and composition of MpM of 91.49%, while HI is 8.51%. MpM is the dominant nematocyst in Pocillopora sp. Future research is recommended to identify this coral at the species level and to study more deeply the various coral species in the genus Pocillopora based on the types and morphology of nematocysts that are useful in the taxonomy of Scleractinia corals.Keywords: Gorontalo, HI, MpM, nematocyst, Pocillopora sp., Scleractinian coral
{"title":"KOMPOSISI TIPE NEMATOSIT KARANG SCLERACTINIA, Pocillopora sp. DARI TERUMBU KARANG PANTAI TONTAYUO, BATUDAA PANTAI, GORONTALO","authors":"Ajrin Y Nusi, C. Paruntu, A. Rumengan, Farnis B. Boneka, N. Gustaf F. Mamangkey, L. T. X. Lalamentik","doi":"10.35800/jplt.9.3.2021.36067","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36067","url":null,"abstract":"The objective of this present study is to know the composition of nematocyst types from the coral Scleractinia, Pocillopora sp. The coral colonies of Pocillopora sp. were collected at Tontayuo coastal coral reefs, Batudaa Pantai, Gorontalo. This research was conducted for 3 months, from September-December 2020. The method of testing the type and composition of nematocysts was carried out using an Olympus CX41 type microscope with x100 objective lens magnification connected to a computer ST24 monitor equipped with optical view 7 application (software). Photos of Pocillopora sp. both alive and skeleton form are documented in this journal. The results of the current study showed that Pocillopora sp. has the types and composition of MpM of 91.49%, while HI is 8.51%. MpM is the dominant nematocyst in Pocillopora sp. Future research is recommended to identify this coral at the species level and to study more deeply the various coral species in the genus Pocillopora based on the types and morphology of nematocysts that are useful in the taxonomy of Scleractinia corals.Keywords: Gorontalo, HI, MpM, nematocyst, Pocillopora sp., Scleractinian coral","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90100174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36520
Yunita Baramuli, Nickson J Kawung, James J. H. Paulus, I. Rumengan, B. Wagey, Fransine B. Manginsela
The aim of this research is to test the potency of Sea cucumber crude extract Holothuria atra using the shrimp larvae Artemia salina Leach. The sample were take from waters of tateli at Minahasa District. Sample preparation and the potential test were done at biology moleculer and marien pharmacy laboratorium of Faculty of Fhiseries and Marine Science Sam Ratulangi University. The sample macerated once with ethanol for 24 hours. Extract of the sample were conentrated using rotary evaporator. Using the Brine Shrimp Lethality Test (BSLT) method with shrimp larvae Artemia salina, Leach. As the sample dry weight of the sample is 420 g after macerated with ethanol then decreased to 18 g after evaporated. Probit analysis of larva mortalityt data as the indicator of anti-cancer potential shown the value LC5030.01 mg/l. Bioactive compounds of sea cucumber H. arta has potency to be develop as anti-cancer material. Keywords: Anti cancer, Sea cucumber, Holothuria atra, Artemia salina leach
{"title":"Uji Potensi Ekstrak Kasar Teripang Laut Holothuria atra Untuk Anti Kanker Dengan Menggunakan Metode Brine Shirmp Lethality Test","authors":"Yunita Baramuli, Nickson J Kawung, James J. H. Paulus, I. Rumengan, B. Wagey, Fransine B. Manginsela","doi":"10.35800/jplt.9.3.2021.36520","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36520","url":null,"abstract":"The aim of this research is to test the potency of Sea cucumber crude extract Holothuria atra using the shrimp larvae Artemia salina Leach. The sample were take from waters of tateli at Minahasa District. Sample preparation and the potential test were done at biology moleculer and marien pharmacy laboratorium of Faculty of Fhiseries and Marine Science Sam Ratulangi University. The sample macerated once with ethanol for 24 hours. Extract of the sample were conentrated using rotary evaporator. Using the Brine Shrimp Lethality Test (BSLT) method with shrimp larvae Artemia salina, Leach. As the sample dry weight of the sample is 420 g after macerated with ethanol then decreased to 18 g after evaporated. Probit analysis of larva mortalityt data as the indicator of anti-cancer potential shown the value LC5030.01 mg/l. Bioactive compounds of sea cucumber H. arta has potency to be develop as anti-cancer material. Keywords: Anti cancer, Sea cucumber, Holothuria atra, Artemia salina leach ","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"16 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89981111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.38430
Kristoper A Lidongi, Chatrien Annita Luzianna Sinjal, Kurniati Kemer, R. Mangindaan, G. Gerung, Sammy Londong
Indonesia has abundant biological resources that produce many potential bioactive compounds that are useful for pharmaceuticals and other materials, one of which is antioxidants. Antioxidants are substances that can prevent or slow down cell damage caused by free radicals. This study aims to analyze how strong the antioxidant activity is in the body of asteroidea (Linckia laevigata) taken in Tongkaina waters, Bunaken District, Manado City. And this study found that the inhibition value of the ethanolic extract of Linckia laevigata had strong antioxidant activity where the extract increased and the concentration was 10 mg/L with an average value of 67,3 %, a concentration of 15 mg/L of 67,8 %, a concentration of 20 mg/L of 68,8 % and the highest concentration of 25 mg/L getting an average value of 69 %. The increace in the percentage of inhibition and the ethanolic extract of Linckia laevigata, showed that the concentration of the extract contributed greatly to the ability of the extract to reduce free radicals. In this study, the DPPH test was carried out by observing the decrease in absorbance wavelength of 517 nm using UV-Vis spectrophotometer,where the decrease in absorbance value as a result and a decrease in color intensity. While vitamin C as a comparison has a greater yield and sample extract of Linckia laevigata which proves that the antioxidant content and the sample has a fairly stable result.Keywords: Bioaktif compounds, Linckia laevigata, Antioxidant, Vitamin C
{"title":"UJI AKTIVITAS ANTIOKSIDAN PADA ASTEROIDEA (Linckia laevigata) DARI PERAIRAN TONGKAINA KECAMATAN BUNAKEN KOTA MANADO","authors":"Kristoper A Lidongi, Chatrien Annita Luzianna Sinjal, Kurniati Kemer, R. Mangindaan, G. Gerung, Sammy Londong","doi":"10.35800/jplt.9.3.2021.38430","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.38430","url":null,"abstract":"Indonesia has abundant biological resources that produce many potential bioactive compounds that are useful for pharmaceuticals and other materials, one of which is antioxidants. Antioxidants are substances that can prevent or slow down cell damage caused by free radicals. This study aims to analyze how strong the antioxidant activity is in the body of asteroidea (Linckia laevigata) taken in Tongkaina waters, Bunaken District, Manado City. And this study found that the inhibition value of the ethanolic extract of Linckia laevigata had strong antioxidant activity where the extract increased and the concentration was 10 mg/L with an average value of 67,3 %, a concentration of 15 mg/L of 67,8 %, a concentration of 20 mg/L of 68,8 % and the highest concentration of 25 mg/L getting an average value of 69 %. The increace in the percentage of inhibition and the ethanolic extract of Linckia laevigata, showed that the concentration of the extract contributed greatly to the ability of the extract to reduce free radicals. In this study, the DPPH test was carried out by observing the decrease in absorbance wavelength of 517 nm using UV-Vis spectrophotometer,where the decrease in absorbance value as a result and a decrease in color intensity. While vitamin C as a comparison has a greater yield and sample extract of Linckia laevigata which proves that the antioxidant content and the sample has a fairly stable result.Keywords: Bioaktif compounds, Linckia laevigata, Antioxidant, Vitamin C","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72650049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36713
Meilisa Mandolang, Janny D Kusen, Veibe Warouw, James J. H. Paulus, Unstain Nwj Rember
This study aims to determine the structure of the target fish community, including species composition, type of research and to analyze the ecological index in which the diversity index, uniformity index, dominance index, and community similarity index. Data collection was carried out in coral reef ecosystems in the traditional zones of Madolin, Alung Banua, Sachiko, Muka Kampung, and East Bunaken areas, all data collection areas were included in the Bunaken National Park area. Data was taken using the Underwater Visual Census method, also equipped with a documentation search for underwater photography and videography carried out at the Marine Biology laboratory, Faculty of Fisheries and Marine Sciences, UNSRAT. There are 3 families, 21 species and 130 individuals at the Madolin area, 5 families, 24 species, and 72 individuals at the Alung Banua area, 5 families, 23 species, and 77 individuals at the Sachiko area, 2 families, 11 species and 129 individuals at the Sachiko area. Muka Kampung, and 4 families, 18 species, and 160 individuals at the East Bunaken location. The calculated ecological index values obtained are H' 2.11 (Madolin), 2.90 (Alung Banua), 2.65 (Sachiko), 1.57 (Muka Kampung), 1.67 (East Bunaken) indicating the ecological condition of the ecosystem. coral reefs are still stable even though diversity is classified as moderate but inversely proportional to low dominance ranging from 0.07 to 0.33 on average. The results of the community similarity analysis using the Sorense index (<50%) showed that there was no similarity in reef fish communities between the 4 stations, namely (Madolin & Alung Banua), (Madolin & Muka Kampung), and (Muka Kampung & East Bunaken). Keywords : target fish community, diversity, dominance, community similarity
{"title":"STRUKTUR KOMUNITAS IKAN TARGET DI EKOSISTEM TERUMBU KARANG PADA ZONA TRADISIONAL PULAU BUNAKEN, TAMAN NASIONAL BNAKEN","authors":"Meilisa Mandolang, Janny D Kusen, Veibe Warouw, James J. H. Paulus, Unstain Nwj Rember","doi":"10.35800/jplt.9.3.2021.36713","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36713","url":null,"abstract":"This study aims to determine the structure of the target fish community, including species composition, type of research and to analyze the ecological index in which the diversity index, uniformity index, dominance index, and community similarity index. Data collection was carried out in coral reef ecosystems in the traditional zones of Madolin, Alung Banua, Sachiko, Muka Kampung, and East Bunaken areas, all data collection areas were included in the Bunaken National Park area. Data was taken using the Underwater Visual Census method, also equipped with a documentation search for underwater photography and videography carried out at the Marine Biology laboratory, Faculty of Fisheries and Marine Sciences, UNSRAT. There are 3 families, 21 species and 130 individuals at the Madolin area, 5 families, 24 species, and 72 individuals at the Alung Banua area, 5 families, 23 species, and 77 individuals at the Sachiko area, 2 families, 11 species and 129 individuals at the Sachiko area. Muka Kampung, and 4 families, 18 species, and 160 individuals at the East Bunaken location. The calculated ecological index values obtained are H' 2.11 (Madolin), 2.90 (Alung Banua), 2.65 (Sachiko), 1.57 (Muka Kampung), 1.67 (East Bunaken) indicating the ecological condition of the ecosystem. coral reefs are still stable even though diversity is classified as moderate but inversely proportional to low dominance ranging from 0.07 to 0.33 on average. The results of the community similarity analysis using the Sorense index (<50%) showed that there was no similarity in reef fish communities between the 4 stations, namely (Madolin & Alung Banua), (Madolin & Muka Kampung), and (Muka Kampung & East Bunaken). Keywords : target fish community, diversity, dominance, community similarity ","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85955106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36920
W. Pelle, Adnan Wantazen, S. Undap, Edwin L. A. Ngangi
The long-term goal of this research is the management of vaname shrimp culture that is certified "Good Fish Cultivation" (GFC) based on environmentally friendly and sustainable technology so that its products can be accepted in domestic and foreign markets. The specific target is the implementation of phytoremediation technology in shrimp aquaculture at the Marine Field Station owned by FPIK UNSRAT which will be developed into the North Sulawesi Marine Education Center (NSMEC) area. The long-term goals and specific targets above will support the leading areas of maritime and maritime affairs in the Unsrat Research Master Plan. Analysis of water quality parameters and shrimp growth response using controlled-scale phytoremediation technology was carried out to see the remediation ability of Eucheuma denticulatum seaweed. Research method using phytoremediation technology application on white shrimp cultivation in the form of demonstration plots. The average growth in the plots provided with seaweed ranged from 8.3 g to 8.4 g. While the control that was not stocked with seaweed, the average weight growth was 6.6 g. This figure shows quite different values for different treatments. Water quality parameters such as DO, pH showed better water quality conditions in the plots spread with seaweed. This shows that Eucheuma denticulatum seaweed is capable of being a phytoremediator for the environment where the vannamei shrimp (Litopenaeus vannamei) is present. Seaweed Eucheuma denticulatum has the ability to absorb waste such as the faces of white shrimp (Litopenaeus vannamei). Keywords: phytoremediation, seaweed, vaname shrimp, East Likupang.
{"title":"RUMPUT LAUT Eucheuma denticulatum SEBAGAI FITOREMEDIATOR PERTUMBUHAN UDANG VANAME (Litopenaeus vannamei)","authors":"W. Pelle, Adnan Wantazen, S. Undap, Edwin L. A. Ngangi","doi":"10.35800/jplt.9.3.2021.36920","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36920","url":null,"abstract":"The long-term goal of this research is the management of vaname shrimp culture that is certified \"Good Fish Cultivation\" (GFC) based on environmentally friendly and sustainable technology so that its products can be accepted in domestic and foreign markets. The specific target is the implementation of phytoremediation technology in shrimp aquaculture at the Marine Field Station owned by FPIK UNSRAT which will be developed into the North Sulawesi Marine Education Center (NSMEC) area. The long-term goals and specific targets above will support the leading areas of maritime and maritime affairs in the Unsrat Research Master Plan. Analysis of water quality parameters and shrimp growth response using controlled-scale phytoremediation technology was carried out to see the remediation ability of Eucheuma denticulatum seaweed. Research method using phytoremediation technology application on white shrimp cultivation in the form of demonstration plots. The average growth in the plots provided with seaweed ranged from 8.3 g to 8.4 g. While the control that was not stocked with seaweed, the average weight growth was 6.6 g. This figure shows quite different values for different treatments. Water quality parameters such as DO, pH showed better water quality conditions in the plots spread with seaweed. This shows that Eucheuma denticulatum seaweed is capable of being a phytoremediator for the environment where the vannamei shrimp (Litopenaeus vannamei) is present. Seaweed Eucheuma denticulatum has the ability to absorb waste such as the faces of white shrimp (Litopenaeus vannamei). Keywords: phytoremediation, seaweed, vaname shrimp, East Likupang.","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77221578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.36672
Sabrina I Hengkengbala, Rosita A J Lintang, Deiske A. Sumilat, R. Mangindaan, E. Ginting, Sipriana S. Tumembouw
One type of enzyme whose application is very widespread is protease enzymes. Microorganisms that have not been widely explored as a source of protease enzymes are bacteria that are in conjunction with Nudibranch. The study aims to identify the morphological characteristics of bacteria, then determine the hydrolysis ability of protease enzymes from bacteria symbiont Nudibranch. Identification of morphological characteristics is done macroscopic, and microscopic with gram staining. Proteolytic activity tests are performed qualitatively by measuring the proteolytic index of bacteria grown on Skim Milk Agar medium. The results of macroscopic identification of symbiotic bacteria with Nudibranch are dominated by irregular colony shapes, undulated edges, growth of the entire colony has a flat height, milky white colony color, buttery texture and opaque bacterial colonies. Based on microscopic identification, two gram-negative isolates are obtained, namely RL.S14, S1.1.1, and gram positive isolates, namely AK.S00, AK.S01, AK.S02, AK.S03, S.PP1, RL.S6, S2.1.5, S2.2.1, S3.3.1, S3.3.4, RL.S10. Nudibranch symbiont Bacterial has colony cells were dominated by the diplobacillus form. The results of the protease activity test of Nudibranch symbiont bacteria showed that 12 positive isolates had proteolytic activity with the highest proteolytic index produced by isolate AK.S00, which was 2.5 mm. Keywords: Nudibranch, symbiotic bacteria, characteristics, protease
{"title":"KARAKTERISTIK MORFOLOGI DAN AKTIVITAS ENZIM PROTEASE BAKTERI SIMBION NUDIBRANCH","authors":"Sabrina I Hengkengbala, Rosita A J Lintang, Deiske A. Sumilat, R. Mangindaan, E. Ginting, Sipriana S. Tumembouw","doi":"10.35800/jplt.9.3.2021.36672","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.36672","url":null,"abstract":"One type of enzyme whose application is very widespread is protease enzymes. Microorganisms that have not been widely explored as a source of protease enzymes are bacteria that are in conjunction with Nudibranch. The study aims to identify the morphological characteristics of bacteria, then determine the hydrolysis ability of protease enzymes from bacteria symbiont Nudibranch. Identification of morphological characteristics is done macroscopic, and microscopic with gram staining. Proteolytic activity tests are performed qualitatively by measuring the proteolytic index of bacteria grown on Skim Milk Agar medium. The results of macroscopic identification of symbiotic bacteria with Nudibranch are dominated by irregular colony shapes, undulated edges, growth of the entire colony has a flat height, milky white colony color, buttery texture and opaque bacterial colonies. Based on microscopic identification, two gram-negative isolates are obtained, namely RL.S14, S1.1.1, and gram positive isolates, namely AK.S00, AK.S01, AK.S02, AK.S03, S.PP1, RL.S6, S2.1.5, S2.2.1, S3.3.1, S3.3.4, RL.S10. Nudibranch symbiont Bacterial has colony cells were dominated by the diplobacillus form. The results of the protease activity test of Nudibranch symbiont bacteria showed that 12 positive isolates had proteolytic activity with the highest proteolytic index produced by isolate AK.S00, which was 2.5 mm. Keywords: Nudibranch, symbiotic bacteria, characteristics, protease","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88140406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-04DOI: 10.35800/jplt.9.3.2021.38609
Maykel R Munaimbala, Carolus P. Paruntu, Anton P Rumengan
The purpose of this study was to examine the structure of the mangrove community on the coast of Tatengesan, Posomaen District, Southeast Minahasa Regency, North Sulawesi. The data collection of the mangrove community structure used the line transect quadratic method measuring 10 m x 10 m 5 times. The research implementation period is 6 (six) months in 2021, starting from the preparation of proposals, data analysis, examination of research results, and the implementation of thesis examinations. Based on the results of the study, found 3 types of mangroves, namely: Rhizophora apiculata, Rhizophora mucronata, and Bruguiera gymnorrhiza. The value of the highest species relative density was found on transect 2 with a value of 88.33% by R. mucronata. For the value of the highest relative frequency of species on transects 1,2 and 3 by R. mucronata, namely 8.3 cm/m. The highest relative species cover was R. mucronata with a value of 76.99% on transect 2. The highest important value index was found in R. mucronata, which was 220.88 on transect 2. The highest diversity index was found on transect 3 with a value of 0.85. The highest evenness index was found on transect 1 with a value of 0.99. For further research, it is necessary to conduct an inventory of mangrove species using the roaming method to find out all the species in the research location.Keywords: Mangrove, Tatengesan, Community, Diversity and Evenness
本研究的目的是研究北苏拉威西米纳哈萨省东南部Posomaen区Tatengesan海岸的红树林群落结构。红树林群落结构数据采集采用10 m × 10 m 5次样线二次法。研究实施周期为2021年6个月，从提案的准备、数据分析、研究成果的检验、论文考试的实施开始。根据研究结果，发现了3种红树类型，分别是:尖根红树(Rhizophora apiculata)、粗根红树(Rhizophora mucronata)和裸树红树(Bruguiera gymnorrhiza)。样带2种相对密度最高，为88.33%;在样条1、2和3上，长绒柽柳的最高物种相对频率为8.3 cm/m。在样带2上相对盖度最高的是长绒田鼠，为76.99%。重要值指数最高的是麻麻，在样条2上为220.88。样带3的多样性指数最高，为0.85。样条1的均匀度指数最高，为0.99。为了进一步研究，有必要利用漫游法对红树林物种进行清查，以找出研究地点的所有物种。关键词:红树林，达腾格桑，群落，多样性和均匀度
{"title":"STRUKTUR KOMUNITAS MANGROVE DI PESISIR DESA TATENGESAN, KECAMATAN POSOMAEN, KABUPATEN MINAHASA TENGGARA","authors":"Maykel R Munaimbala, Carolus P. Paruntu, Anton P Rumengan","doi":"10.35800/jplt.9.3.2021.38609","DOIUrl":"https://doi.org/10.35800/jplt.9.3.2021.38609","url":null,"abstract":"The purpose of this study was to examine the structure of the mangrove community on the coast of Tatengesan, Posomaen District, Southeast Minahasa Regency, North Sulawesi. The data collection of the mangrove community structure used the line transect quadratic method measuring 10 m x 10 m 5 times. The research implementation period is 6 (six) months in 2021, starting from the preparation of proposals, data analysis, examination of research results, and the implementation of thesis examinations. Based on the results of the study, found 3 types of mangroves, namely: Rhizophora apiculata, Rhizophora mucronata, and Bruguiera gymnorrhiza. The value of the highest species relative density was found on transect 2 with a value of 88.33% by R. mucronata. For the value of the highest relative frequency of species on transects 1,2 and 3 by R. mucronata, namely 8.3 cm/m. The highest relative species cover was R. mucronata with a value of 76.99% on transect 2. The highest important value index was found in R. mucronata, which was 220.88 on transect 2. The highest diversity index was found on transect 3 with a value of 0.85. The highest evenness index was found on transect 1 with a value of 0.99. For further research, it is necessary to conduct an inventory of mangrove species using the roaming method to find out all the species in the research location.Keywords: Mangrove, Tatengesan, Community, Diversity and Evenness","PeriodicalId":17792,"journal":{"name":"JURNAL PESISIR DAN LAUT TROPIS","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80057609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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