Pub Date : 2024-07-01DOI: 10.31002/jtoi.v17i1.1150
R. Ririn, Wicaksaning Dyah Pamungki, Fitri Mustiko Ningsih
Avocado leaf ethanol extract as a traditional medicine has been proven to have various pharmacological activities, but the safety of its use is not yet known. This study aims to determine the range of LD50 values, toxic symptoms, changes in body weight, and SGPT SGOT levels of Balb/c mice. The research was carried out experimentally using a fixed dose toxicity test method with a randomized matched pre and post-test control group design. EEDA is made by 70% ethanol maceration. The acute toxicity test consists of 2 stages: preliminary and main tests. Preliminary tests were carried out with a dose of extract 300 mg/kg BW once administered. After 24 hours of observation, there were no toxic symptoms, the test dose was increased to 2000 mg/kg BW. The main test was carried out using 10 test animals which were divided into 2 groups, each given a dose of extract 2000 mg/kg BW once and CMC Na 1% (negative control). Observation of toxic symptoms and death was carried out for 14 days. The LD50 value is determined from the results of toxic symptoms and death of test animals. Toxic symptoms were analyzed descriptively, and changes in body weight and SGOT SGPT levels were analyzed statistically (95% confidence level). The research results show that avocado leaf ethanol extract has an LD50 value range of >2000 mg/kg BW. The test dose of 2000 mg/kg BW did not cause toxic symptoms and death. SGOT and SGPT levels were still within normal limits, however, changes in SGPT levels were significantly different from controls. Thus, an extract dose of 2000 mg/kg BW has an acute toxic effect on the liver of mice.
{"title":"Uji Toksisitas Akut Ekstrak Etanol Daun Alpukat (Persea americana Mill.) dan Pengaruhnya Terhadap Hepar Mencit BALB/c","authors":"R. Ririn, Wicaksaning Dyah Pamungki, Fitri Mustiko Ningsih","doi":"10.31002/jtoi.v17i1.1150","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.1150","url":null,"abstract":"Avocado leaf ethanol extract as a traditional medicine has been proven to have various pharmacological activities, but the safety of its use is not yet known. This study aims to determine the range of LD50 values, toxic symptoms, changes in body weight, and SGPT SGOT levels of Balb/c mice. The research was carried out experimentally using a fixed dose toxicity test method with a randomized matched pre and post-test control group design. EEDA is made by 70% ethanol maceration. The acute toxicity test consists of 2 stages: preliminary and main tests. Preliminary tests were carried out with a dose of extract 300 mg/kg BW once administered. After 24 hours of observation, there were no toxic symptoms, the test dose was increased to 2000 mg/kg BW. The main test was carried out using 10 test animals which were divided into 2 groups, each given a dose of extract 2000 mg/kg BW once and CMC Na 1% (negative control). Observation of toxic symptoms and death was carried out for 14 days. The LD50 value is determined from the results of toxic symptoms and death of test animals. Toxic symptoms were analyzed descriptively, and changes in body weight and SGOT SGPT levels were analyzed statistically (95% confidence level). The research results show that avocado leaf ethanol extract has an LD50 value range of >2000 mg/kg BW. The test dose of 2000 mg/kg BW did not cause toxic symptoms and death. SGOT and SGPT levels were still within normal limits, however, changes in SGPT levels were significantly different from controls. Thus, an extract dose of 2000 mg/kg BW has an acute toxic effect on the liver of mice.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141691307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.31002/jtoi.v17i1.1159
Indriana Noor Istiqomah, Mashuri, Achlish Abdillah, Laili Nur Azizah
Recent studies have focused on exploring natural remedies to accelerate wound healing. One such herbal plant is the Ganjan plant (Artemisia vulgaris), which contains essential oils, coumarins, flavonoids, triterpenoids, and phenolic acids, which make it a good candidate for natural remedies. This study analyzed the wound-healing potential of the Ganjan plant using a true experimental research design on 16 male Sprague Dawley rats. The rats were divided into four groups and subjected to different treatments, including a negative control group without treatment (K1), a 10% Ganjan herbal extract ointment group (K2), a 30% Ganjan herbal extract ointment group (K3), and a positive control group with 10% betadine ointment (K4). The ointments were applied once daily for 21 days or until the wounds healed, and wound area measurements were taken every three days. The results showed that the 10% and 30% Ganjan ointments effectively reduced the wound area, and there was no difference in wound contraction between 10% and 30% Ganjan ointment and 10% Betadine. The study concludes that Ganjan plant extract ointment is an effective natural remedy for wound healing and that the best results are seen in the 30% Ganjan extract ointment group.
{"title":"Potensi Tanaman Ganjan (Artemisia vulgaris) dalam Penyembuhan Luka Sayat pada Tikus (Sprague Dawley)","authors":"Indriana Noor Istiqomah, Mashuri, Achlish Abdillah, Laili Nur Azizah","doi":"10.31002/jtoi.v17i1.1159","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.1159","url":null,"abstract":"Recent studies have focused on exploring natural remedies to accelerate wound healing. One such herbal plant is the Ganjan plant (Artemisia vulgaris), which contains essential oils, coumarins, flavonoids, triterpenoids, and phenolic acids, which make it a good candidate for natural remedies. This study analyzed the wound-healing potential of the Ganjan plant using a true experimental research design on 16 male Sprague Dawley rats. The rats were divided into four groups and subjected to different treatments, including a negative control group without treatment (K1), a 10% Ganjan herbal extract ointment group (K2), a 30% Ganjan herbal extract ointment group (K3), and a positive control group with 10% betadine ointment (K4). The ointments were applied once daily for 21 days or until the wounds healed, and wound area measurements were taken every three days. The results showed that the 10% and 30% Ganjan ointments effectively reduced the wound area, and there was no difference in wound contraction between 10% and 30% Ganjan ointment and 10% Betadine. The study concludes that Ganjan plant extract ointment is an effective natural remedy for wound healing and that the best results are seen in the 30% Ganjan extract ointment group.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141714259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.31002/jtoi.v17i1.1155
Farida Yuliani, Fadila Rona Nureida, Fazat Fairuzia, Eva Hana Rosidah, Agung Wahyu Nugroho, Afifatul Amiroh
In 2020, malaria cases worldwide increased from 219 million cases to 241 million cases with 627 thousand deaths. This creates problems in terms of the availability of malaria drugs. In addition, resistance to Plasmodium sp. against the commonly used malaria drug chloroquine is another problem. Therefore, in the treatment of malaria, combination-based treatment with artemisinin is highly recommended. However, there are obstacles in the production of artemisinin because its content in plants is relatively low. Therefore, this research aims to increase artemisinin content using endophytic bacteria. The method used is a factorial design with 2 factors, namely Accessions (Green & Purple) and endophytic bacteria concentration (0%, 1%, and 2%). Endophytic bacteria isolated from artemisinin plants were first cultured in a nutrient-rich medium to increase their population. Then, they were inoculated by spraying them twice a week for two months with each accession in each treatment combination. The results showed that inoculation with endophytic bacteria had the best concentration in increasing artemisinin content, namely 1% in green and purple Artemisia accessions. Artemisinin inoculation of 1% was able to increase artemisinin by 27.85% compared to control plants in green Artemisia and purple Artemisia accessions with an increase of 32.89% compared to control plants. The best concentration of endophytic bacteria in increasing artemisinin content is 1%.
{"title":"Increasing Artemisinin Content on Artemisia Plants Through Endophytic Bacteria Inoculation as An Effort to Support the Availability of Malaria Drugs","authors":"Farida Yuliani, Fadila Rona Nureida, Fazat Fairuzia, Eva Hana Rosidah, Agung Wahyu Nugroho, Afifatul Amiroh","doi":"10.31002/jtoi.v17i1.1155","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.1155","url":null,"abstract":"In 2020, malaria cases worldwide increased from 219 million cases to 241 million cases with 627 thousand deaths. This creates problems in terms of the availability of malaria drugs. In addition, resistance to Plasmodium sp. against the commonly used malaria drug chloroquine is another problem. Therefore, in the treatment of malaria, combination-based treatment with artemisinin is highly recommended. However, there are obstacles in the production of artemisinin because its content in plants is relatively low. Therefore, this research aims to increase artemisinin content using endophytic bacteria. The method used is a factorial design with 2 factors, namely Accessions (Green & Purple) and endophytic bacteria concentration (0%, 1%, and 2%). Endophytic bacteria isolated from artemisinin plants were first cultured in a nutrient-rich medium to increase their population. Then, they were inoculated by spraying them twice a week for two months with each accession in each treatment combination. The results showed that inoculation with endophytic bacteria had the best concentration in increasing artemisinin content, namely 1% in green and purple Artemisia accessions. Artemisinin inoculation of 1% was able to increase artemisinin by 27.85% compared to control plants in green Artemisia and purple Artemisia accessions with an increase of 32.89% compared to control plants. The best concentration of endophytic bacteria in increasing artemisinin content is 1%.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141697610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.31002/jtoi.v17i1.1192
Mery Budiarti, Nur Rahmawati Wijaya, W. Jokopriyambodo
Moringa oleifera Lam. is in high demand as a raw material for pharmaceutical constituents and dietary supplements, particularly for producing stunting supplements. However, recommendations regarding M. oleifera quality standards have not supported this claim as scientific evidence of its safety and effectiveness. This research intends to expand knowledge regarding the quality standards of M. oleifera leaves, particularly their chemical properties. Height-variant samples of M. oleifera were collected from six locations in the Central Java, Indonesia. A hierarchical cluster analysis (HCA) was performed to group the most each parameter's values. The parameters included water-soluble extract, ethanol-soluble extract, total ash content, acid-insoluble ash, total flavonoid content was quantified as quercetin, and thin-layer chromatography (TLC) fingerprint. The suggestion of specific value for each parameter as a future reference for M. oleifera, including water- and ethanol-soluble extract content of at least 41% and 19%, and a total- and acid-insoluble ash content of a maximum of 11% and 4%, respectively. The minimal quercetin contains as the chemical marker compound of M. oleifera was a minimum of 1.285%. The chromatogram profile of TLC suggested 7 (254 nm) and 8 (366 nm) spots.
{"title":"Chemical Characterization of Moringa oleifera Lam. from Six Growth Locations in Central of Java: An Initiation of Standardization `","authors":"Mery Budiarti, Nur Rahmawati Wijaya, W. Jokopriyambodo","doi":"10.31002/jtoi.v17i1.1192","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.1192","url":null,"abstract":"Moringa oleifera Lam. is in high demand as a raw material for pharmaceutical constituents and dietary supplements, particularly for producing stunting supplements. However, recommendations regarding M. oleifera quality standards have not supported this claim as scientific evidence of its safety and effectiveness. This research intends to expand knowledge regarding the quality standards of M. oleifera leaves, particularly their chemical properties. Height-variant samples of M. oleifera were collected from six locations in the Central Java, Indonesia. A hierarchical cluster analysis (HCA) was performed to group the most each parameter's values. The parameters included water-soluble extract, ethanol-soluble extract, total ash content, acid-insoluble ash, total flavonoid content was quantified as quercetin, and thin-layer chromatography (TLC) fingerprint. The suggestion of specific value for each parameter as a future reference for M. oleifera, including water- and ethanol-soluble extract content of at least 41% and 19%, and a total- and acid-insoluble ash content of a maximum of 11% and 4%, respectively. The minimal quercetin contains as the chemical marker compound of M. oleifera was a minimum of 1.285%. The chromatogram profile of TLC suggested 7 (254 nm) and 8 (366 nm) spots.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141710324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ajeng Puspo Aji, Rochany Septianingsih, Septiana Indratmoko, Asep Nurrahman Y, Lisa Indriana
Erectile dysfunction (ED) refers to medical issues concerning men's sexual health. Purwaceng, pasak bumi, and epimedium plants contain aphrodisiac compounds that can boost stamina, libido (sexual desire), and male fertility. The formula for microemulsions is 80: PEG 400: oil shavings in a ratio of 73.6711: 12.5705: 13.7584, with icariin, purwaceng, and pasak bumi added. The microemulsion system was created because it contained a large number of active ingredients. The stability of a microemulsion is further influenced by surfactants (tween 80) and cosurfactants (PEG 400) as emulsifiers, as well as the oil used as a solvent for the active component (bottled oil). The goal of this study was to create a microemulsion that would mix icariin, pasak bumi, and purwaceng substances to improve solubility and synergize pharmacological effects. The formulation is optimized using Simplex Lattice Design (SLD) to produce the optimal formula. The microemulsion formulations of icariin, pasak bumi extract, and purwaceng extract produce optimal microemulsions and enter into good microemulsion susceptibility. They can also increase the aphrodisiac effectiveness when compared to the administration of the single active substance icariin, pasak bumi extract, and purwaceng extract. As a result, this product exhibits microemulsion capabilities, allowing it to minimize dose while increasing effect.
勃起功能障碍（ED）是指与男性性健康有关的医学问题。Purwaceng、pasak bumi 和 epimedium 植物含有壮阳化合物，可增强体力、性欲（性欲）和男性生育能力。微乳剂的配方为 80：PEG 400：油屑，比例为 73.6711：12.5705：13.7584，其中添加了冰片甙、紫花地丁和附子。微乳液体系的产生是因为它含有大量的活性成分。作为乳化剂的表面活性剂（吐温 80）和助表面活性剂（PEG 400）以及作为活性成分溶剂的油（瓶装油）都会进一步影响微乳液的稳定性。本研究的目的是制作一种微乳液，将冰片苷、巴沙克布米和蒲公英物质混合在一起，以提高溶解度和协同药理作用。该配方采用简单网格设计（SLD）进行优化，以获得最佳配方。冰片苷、巴沙棘提取物和马钱子提取物的微乳剂配方能产生最佳微乳剂，并具有良好的微乳剂易感性。与服用单一活性物质冰片苷、巴沙枯木提取物和马钱子提取物相比，它们还能提高壮阳效果。因此，本产品具有微乳化能力，可在增加效果的同时减少剂量。
{"title":"Peningkatan Aktivitas Afrodisiak Mikroemulsi Icariin, Ekstrak Purwaceng dan Ekstrak Pasak Bumi","authors":"Ajeng Puspo Aji, Rochany Septianingsih, Septiana Indratmoko, Asep Nurrahman Y, Lisa Indriana","doi":"10.31002/jtoi.v17i1.984","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.984","url":null,"abstract":"Erectile dysfunction (ED) refers to medical issues concerning men's sexual health. Purwaceng, pasak bumi, and epimedium plants contain aphrodisiac compounds that can boost stamina, libido (sexual desire), and male fertility. The formula for microemulsions is 80: PEG 400: oil shavings in a ratio of 73.6711: 12.5705: 13.7584, with icariin, purwaceng, and pasak bumi added. The microemulsion system was created because it contained a large number of active ingredients. The stability of a microemulsion is further influenced by surfactants (tween 80) and cosurfactants (PEG 400) as emulsifiers, as well as the oil used as a solvent for the active component (bottled oil). The goal of this study was to create a microemulsion that would mix icariin, pasak bumi, and purwaceng substances to improve solubility and synergize pharmacological effects. The formulation is optimized using Simplex Lattice Design (SLD) to produce the optimal formula. The microemulsion formulations of icariin, pasak bumi extract, and purwaceng extract produce optimal microemulsions and enter into good microemulsion susceptibility. They can also increase the aphrodisiac effectiveness when compared to the administration of the single active substance icariin, pasak bumi extract, and purwaceng extract. As a result, this product exhibits microemulsion capabilities, allowing it to minimize dose while increasing effect.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141707528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.31002/jtoi.v17i1.1156
Aryo Tedjo
Acetogenin derived from soursop (Annona muricata) leaves are known to have antidiabetic and anticancer activities. Nevertheless, there has been no study related to the compounds found in A. muricata leaves, such as acetogenin, as SGLT2 inhibitors. This research aims to investigate the activity of acetogenin compounds as SGLT2 inhibitors while maintaining low selectivity against SGLT1 using molecular docking methods using Molegro Virtual Docker (MVD). Based on the Rerank score, five acetogenin compounds, namely muricin H, annonacin A, annopentocin B, murihexocin C, and corossolone, are predicted to be SGLT2 inhibitors with better selectivity compared to empagliflozin. Among these five compounds, muricin H and corossolone exhibit the most similarity in interaction with amino acid residues in the SGLT2 A-chain compared to empagliflozin. In silico ADMET analysis results indicate that both compounds have absorption, distribution, and metabolism capabilities, similar to empagliflozin. However, it should be noted that both compounds are more toxic, with muricin H predicted to have hepatotoxic properties.
{"title":"In Silico Study of Acetogenin Compounds from Soursop (Annona muricata) Leaves as Sodium-Glucose Cotransporter-2 (SGLT2) Inhibitors","authors":"Aryo Tedjo","doi":"10.31002/jtoi.v17i1.1156","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.1156","url":null,"abstract":"Acetogenin derived from soursop (Annona muricata) leaves are known to have antidiabetic and anticancer activities. Nevertheless, there has been no study related to the compounds found in A. muricata leaves, such as acetogenin, as SGLT2 inhibitors. This research aims to investigate the activity of acetogenin compounds as SGLT2 inhibitors while maintaining low selectivity against SGLT1 using molecular docking methods using Molegro Virtual Docker (MVD). Based on the Rerank score, five acetogenin compounds, namely muricin H, annonacin A, annopentocin B, murihexocin C, and corossolone, are predicted to be SGLT2 inhibitors with better selectivity compared to empagliflozin. Among these five compounds, muricin H and corossolone exhibit the most similarity in interaction with amino acid residues in the SGLT2 A-chain compared to empagliflozin. In silico ADMET analysis results indicate that both compounds have absorption, distribution, and metabolism capabilities, similar to empagliflozin. However, it should be noted that both compounds are more toxic, with muricin H predicted to have hepatotoxic properties.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141697368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.31002/jtoi.v17i1.1186
Dewi Andini, Kunti Mulangsri, Sevitia Rosella, Isna Azmut Baroroh
Leaves and stem bark of orange jasmine have bioactive compounds such as phenol and flavonoids, which these compounds could be extracted by organic solvent and have antifungal activity against Malassezia furfur which cause excema, dandruff and tinea versicolor in humans. Fractionation of the extract would obtain a specific compound based on the polarity of the solvent. This research aims to know the active fraction of leaves and stem bark of orange jasmine which had antifungal activity against Malassezia furfur. The simplicia powder of leaves and stem bark orange jasmine was macerated with 96% ethanol solvent and then fractionated as gradually with n-hexane, ethyl acetate, and water solvent. The antifungal activity of ethanol extract and fractions was tested against Malassezia furfur with 80; 90; 100% concentration by disk diffusion method at 37˚C for 48 hours of incubation. The data of antifungal activity was analyzed by two-way ANOVA. The result of this research showed antifungal activity from active fraction as water fraction of leaves and stem bark of orange jasmine against Malassezia furfur. The diameter of inhibition zones for the active fraction of leaves and stem bark of orange jasmine such as 8.6 – 14 mm and 12.3 – 15.8 mm respectively.
{"title":"Antifungal Activity the Active Fraction of Orange Jasmine (Murraya paniculata (Linn) Jack) Leaves and Stem Bark Against Malassezia furfur","authors":"Dewi Andini, Kunti Mulangsri, Sevitia Rosella, Isna Azmut Baroroh","doi":"10.31002/jtoi.v17i1.1186","DOIUrl":"https://doi.org/10.31002/jtoi.v17i1.1186","url":null,"abstract":"Leaves and stem bark of orange jasmine have bioactive compounds such as phenol and flavonoids, which these compounds could be extracted by organic solvent and have antifungal activity against Malassezia furfur which cause excema, dandruff and tinea versicolor in humans. Fractionation of the extract would obtain a specific compound based on the polarity of the solvent. This research aims to know the active fraction of leaves and stem bark of orange jasmine which had antifungal activity against Malassezia furfur. The simplicia powder of leaves and stem bark orange jasmine was macerated with 96% ethanol solvent and then fractionated as gradually with n-hexane, ethyl acetate, and water solvent. The antifungal activity of ethanol extract and fractions was tested against Malassezia furfur with 80; 90; 100% concentration by disk diffusion method at 37˚C for 48 hours of incubation. The data of antifungal activity was analyzed by two-way ANOVA. The result of this research showed antifungal activity from active fraction as water fraction of leaves and stem bark of orange jasmine against Malassezia furfur. The diameter of inhibition zones for the active fraction of leaves and stem bark of orange jasmine such as 8.6 – 14 mm and 12.3 – 15.8 mm respectively.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141715202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.22435/jtoi.v15i2.6436
Nur Rahmawati Wijaya, Devi Safrina, Prambayu Brenda Herera, Mery Budiarti
Mentha × piperita L., also known as peppermint, is a plant with various uses, including medicine, cosmetics, and food. Numerous industries have a high need for peppermint products, but Indonesia is currently unable to meet this demand and should continue to import peppermint. One effort can be made to improve cultivation procedures, and tissue culture becomes one alternative. This research uses shoots as explants with Murashige & Skoog's basic media and growth regulators BAP and NAA. The research was conducted in two phases: six-week initial planting and seven-week subculture. The treatment of BAP 4 mg/L + NAA 0.5 mg/L provides better performance for the number of leaves, and BAP 3 mg/L produced the best response regarding the number of shoots. Furthermore, BAP 1 mg/L + NAA 1 mg/L produced the best response to shoot height and number of leaves, and BAP 3 mg/L + NAA 0.5 mg/L generated the best response to root length. Based on the research, BAP 3 mg/L is the optimal treatment.
{"title":"EFFECT OF NAA AND BAP APPLICATION ON THE GROWTH RESPONSES OF Mentha × piperita L.","authors":"Nur Rahmawati Wijaya, Devi Safrina, Prambayu Brenda Herera, Mery Budiarti","doi":"10.22435/jtoi.v15i2.6436","DOIUrl":"https://doi.org/10.22435/jtoi.v15i2.6436","url":null,"abstract":"Mentha × piperita L., also known as peppermint, is a plant with various uses, including medicine, cosmetics, and food. Numerous industries have a high need for peppermint products, but Indonesia is currently unable to meet this demand and should continue to import peppermint. One effort can be made to improve cultivation procedures, and tissue culture becomes one alternative. This research uses shoots as explants with Murashige & Skoog's basic media and growth regulators BAP and NAA. The research was conducted in two phases: six-week initial planting and seven-week subculture. The treatment of BAP 4 mg/L + NAA 0.5 mg/L provides better performance for the number of leaves, and BAP 3 mg/L produced the best response regarding the number of shoots. Furthermore, BAP 1 mg/L + NAA 1 mg/L produced the best response to shoot height and number of leaves, and BAP 3 mg/L + NAA 0.5 mg/L generated the best response to root length. Based on the research, BAP 3 mg/L is the optimal treatment.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88711964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.22435/jtoi.v15i2.5655
Mahendra Wahyu Dewangga, D. Irianto
Atlet dituntut untuk memiliki performa yang baik agar memperoleh prestasi disetiap kompetisi. Latihan fisik teratur memberikan manfaat pada peningkatan performa atlet. Namun, latihan fisik berat dan berlebihan dapat meningkatkan radikal bebas di dalam tubuh. Hal ini dapat menyebabkan stress oksidatif yang berdampak pada penurunan performa atlet. Ubi jalar ungu (Ipomoea batatas var Antin 3) adalah asupan makanan yang memiliki kandungan antosianin yang tinggi dan memiliki potensi untuk menurunkan stress oksidatif para atlet. Tujuan penelitian ini untuk mengetahui potensi ubi jalar ungu (Ipomoea batatas var Antin 3) sebagai asupan antioksidan untuk atlet. Medote penelitian yang digunakan adalah experimental post-test only control group design menggunakan hewan coba berupa tikus galur wistar. Penelitian dilakukan pada bulan Juli 2021 di Integrated Biomedic Laboratory Fakultas Kedokteran Universitas Islam Sultan Agung. Sampel berjumlah 20 tikus yang dibagi menjadi 4 kelompok. Kelompok 1 merupakan kelompok control. Kelompok 2, tikus diberikan latihan fisik berat. Kelompok 3 tikus diberikan latihan fisik berat dan asupan ubi jalar ungu 1,6mg/hari. Kelompok 4, tikus diberikan ubi jalar ungu 3,6mg/hari. Pemberian latihan fisik berupa latihan berenang selama 15-20 menit. Penelitian ini berjalan selama 14 hari. Pada hari ke 15 dilakukan pengambilan serum darah lalu dilakukan pengecekan Malondialdehyde (MDA) dan Superoxide Dismutase (SOD). Hasil analisa one way anova dan post hoc LSD dari pengukuran kadar MDA dan SOD, kelompok 4 memiliki hasil yang yang paling efektif (p<0,05) untuk mencegah penurunan MDA dan peningkatan SOD apabila dibandingkan dengan kelompok 3. Hasil dari kelompok 4 juga yang paling mendekati dengan kelompok 1 (p>0,05). Dapat disimpulkan bahwa ubi jalar ungu (Ipomoea batatas var Antin 3) dapat menurunkan kadar stress oksidatif pasca latihan fisik berlebihan. Ubi jalar ungu (Ipomoea batatas var Antin 3) memiliki potensi sebagai asupan antioksidan untuk atlet.
运动员必须有良好的表现，才能在比赛中取得好成绩。经常锻炼对运动员的表现有好处。然而，剧烈和过度的体育锻炼会增加身体的自由基。这可能会导致氧化压力，导致运动员表现不佳。紫山药(学名Ipomoea batatas var Antin 3)是一种含有高淀粉素含量的食物，有可能减轻运动员的氧化压力。这项研究的目的是确定作为运动员的抗氧化剂摄入的Ipomoea batatas var Antin 3的潜力。Medote的研究是术后唯一的控制小组设计，使用的是鼠形树。该研究于2021年7月在伊斯兰大苏丹大学医学院整合生物医学实验室进行。样本中有20只老鼠被分成4组。第一组是控制组。第二组，老鼠接受严格的体能训练。第三组老鼠每天要进行大量的体能训练和紫甘薯摄入量为1.6毫克/日。第四组，鼠每天注射36mg紫甘薯。体育锻炼是15到20分钟的游泳锻炼。这项研究进行了14天。第15天进行了血清提取，并对马宏迪海德(MDA)和过氧化氢衍生物(SOD)进行了检查。通过MDA和SOD的测量，第四组的测试结果是最有效的(p0.05)。我们可以得出结论，紫红薯(Ipomoea batatas var Antin 3)在过度的体育锻炼后可以降低氧化压力。紫甘薯(Ipomoea batatas var Antin 3)有潜力成为运动员的抗氧化剂摄入量。
{"title":"POTENSI UBI JALAR UNGU (Ipomoea batatas var Antin 3) SEBAGAI ASUPAN ANTIOKSIDAN UNTUK ATLET","authors":"Mahendra Wahyu Dewangga, D. Irianto","doi":"10.22435/jtoi.v15i2.5655","DOIUrl":"https://doi.org/10.22435/jtoi.v15i2.5655","url":null,"abstract":"Atlet dituntut untuk memiliki performa yang baik agar memperoleh prestasi disetiap kompetisi. Latihan fisik teratur memberikan manfaat pada peningkatan performa atlet. Namun, latihan fisik berat dan berlebihan dapat meningkatkan radikal bebas di dalam tubuh. Hal ini dapat menyebabkan stress oksidatif yang berdampak pada penurunan performa atlet. Ubi jalar ungu (Ipomoea batatas var Antin 3) adalah asupan makanan yang memiliki kandungan antosianin yang tinggi dan memiliki potensi untuk menurunkan stress oksidatif para atlet. Tujuan penelitian ini untuk mengetahui potensi ubi jalar ungu (Ipomoea batatas var Antin 3) sebagai asupan antioksidan untuk atlet. Medote penelitian yang digunakan adalah experimental post-test only control group design menggunakan hewan coba berupa tikus galur wistar. Penelitian dilakukan pada bulan Juli 2021 di Integrated Biomedic Laboratory Fakultas Kedokteran Universitas Islam Sultan Agung. Sampel berjumlah 20 tikus yang dibagi menjadi 4 kelompok. Kelompok 1 merupakan kelompok control. Kelompok 2, tikus diberikan latihan fisik berat. Kelompok 3 tikus diberikan latihan fisik berat dan asupan ubi jalar ungu 1,6mg/hari. Kelompok 4, tikus diberikan ubi jalar ungu 3,6mg/hari. Pemberian latihan fisik berupa latihan berenang selama 15-20 menit. Penelitian ini berjalan selama 14 hari. Pada hari ke 15 dilakukan pengambilan serum darah lalu dilakukan pengecekan Malondialdehyde (MDA) dan Superoxide Dismutase (SOD). Hasil analisa one way anova dan post hoc LSD dari pengukuran kadar MDA dan SOD, kelompok 4 memiliki hasil yang yang paling efektif (p<0,05) untuk mencegah penurunan MDA dan peningkatan SOD apabila dibandingkan dengan kelompok 3. Hasil dari kelompok 4 juga yang paling mendekati dengan kelompok 1 (p>0,05). Dapat disimpulkan bahwa ubi jalar ungu (Ipomoea batatas var Antin 3) dapat menurunkan kadar stress oksidatif pasca latihan fisik berlebihan. Ubi jalar ungu (Ipomoea batatas var Antin 3) memiliki potensi sebagai asupan antioksidan untuk atlet.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86702593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.22435/jtoi.v15i2.6210
M. Adi, Devi Safrina, S. Wahyono
Indonesia is among the countries producing medicinal plants (TO) for domestic and foreign demand. Nevertheless, Indonesia keeps importing a variety of TO kinds. One reason for this is farmer's lack of interest in cultivating medicinal plants, which is interesting to observe considering that, in terms of market share, ecology, and geography, all of them support the development of medicinal plant cultivation. This cross-sectional study with observations and interviews aims to get primary data to identify the factors causing the lacking interest of farmers in cultivating medicinal plants. In October 2018, we conducted our data collection. Statistics Indonesia (BPS) provided secondary data on the production and trade of medicinal plants from 2009 to 2020. We did descriptive data analysis. The results showed several factors caused farmers to be less interested in cultivating medicinal plants. Expense fluctuations, the length of the planting period, technology, and access to marketing, until the government's attention has not been optimized are the contributing factors. It should make efforts to increase the interest of farmers in cultivating medicinal plants. There are ways to encourage people to grow medicinal plants as their primary source of income, including stable prices, market accessibility, efficient cultivation, and post-harvest technology.
{"title":"DETERMINANTS OF THE LACK OF INTEREST IN CULTIVATING MEDICINAL PLANTS IN WONOGIRI, CENTRAL JAVA","authors":"M. Adi, Devi Safrina, S. Wahyono","doi":"10.22435/jtoi.v15i2.6210","DOIUrl":"https://doi.org/10.22435/jtoi.v15i2.6210","url":null,"abstract":"Indonesia is among the countries producing medicinal plants (TO) for domestic and foreign demand. Nevertheless, Indonesia keeps importing a variety of TO kinds. One reason for this is farmer's lack of interest in cultivating medicinal plants, which is interesting to observe considering that, in terms of market share, ecology, and geography, all of them support the development of medicinal plant cultivation. This cross-sectional study with observations and interviews aims to get primary data to identify the factors causing the lacking interest of farmers in cultivating medicinal plants. In October 2018, we conducted our data collection. Statistics Indonesia (BPS) provided secondary data on the production and trade of medicinal plants from 2009 to 2020. We did descriptive data analysis. The results showed several factors caused farmers to be less interested in cultivating medicinal plants. Expense fluctuations, the length of the planting period, technology, and access to marketing, until the government's attention has not been optimized are the contributing factors. It should make efforts to increase the interest of farmers in cultivating medicinal plants. There are ways to encourage people to grow medicinal plants as their primary source of income, including stable prices, market accessibility, efficient cultivation, and post-harvest technology.","PeriodicalId":17794,"journal":{"name":"Jurnal Tumbuhan Obat Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86165206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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