Aim. To study the effectiveness of treatment of sialolithiasis by sialolithotripsy. Methods. Between 2015 and 2018, extracorporeal shockwave lithotripsy of salivary stones of 39 patients was performed in the Nasreddin Tusi Memorial Clinic. The stone was located in the submandibular gland and its duct for 33 patients and the parotid salivary glands and its duct for 6 patients. The average age of patients was 50±3.38 (between 22 and 77). Results. Stone fragmentation as a result of sialolithotripsy was achieved in 36 (92.7%) of 39 examined patients (p=0.031). In 3 patients the stone was completely fragmented, but due to the narrow lumen of the main duct, it did not move away. In 3 patients with calculus size more than 2 cm, the stone was not fragmented. According to our results, the necessary effect was achieved in 85% of cases and was unachieved in only 15% of cases. Conclusion. Given the complexity of the surgical treatment in patients with salivary stone and potential postoperative complications, especially with the stone localization within the parotid gland, we concluded that extracorporeal shockwave lithotripsy is a promising alternative treatment for patients with salivary stone.
{"title":"The treatment of sialolithiasis by sialolithotripsy","authors":"S. Aliyev, Алиев Саид Зохраб оглы","doi":"10.17816/kmj2020-586","DOIUrl":"https://doi.org/10.17816/kmj2020-586","url":null,"abstract":"Aim. To study the effectiveness of treatment of sialolithiasis by sialolithotripsy. Methods. Between 2015 and 2018, extracorporeal shockwave lithotripsy of salivary stones of 39 patients was performed in the Nasreddin Tusi Memorial Clinic. The stone was located in the submandibular gland and its duct for 33 patients and the parotid salivary glands and its duct for 6 patients. The average age of patients was 50±3.38 (between 22 and 77). Results. Stone fragmentation as a result of sialolithotripsy was achieved in 36 (92.7%) of 39 examined patients (p=0.031). In 3 patients the stone was completely fragmented, but due to the narrow lumen of the main duct, it did not move away. In 3 patients with calculus size more than 2 cm, the stone was not fragmented. According to our results, the necessary effect was achieved in 85% of cases and was unachieved in only 15% of cases. Conclusion. Given the complexity of the surgical treatment in patients with salivary stone and potential postoperative complications, especially with the stone localization within the parotid gland, we concluded that extracorporeal shockwave lithotripsy is a promising alternative treatment for patients with salivary stone.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73330990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Vaneeva, Ванеева Елена Викторовна, V. Rosin, Росин Виталий Анатольевич, D. Dyakonov, Дьяконов Дмитрий Андреевич, S. Samarina, Самарина Светлана Валерьевна
Aim. To assess the relationship between isolated and combined expression of pSTAT3, pACT1 in tumor cells with the survival of patients with diffuse large B-cell lymphoma (DLBCL). Methods. The study included 100 patients with the first diagnosed diffuse large B-cell lymphoma, observed in the institute's clinic between 2010 and 2018 who received standard first-line R-CHOP (rituximab + cyclophosphamide, doxorubicin, vincristine and prednisone) chemotherapy. The relative number of expressing pSTAT3 and pAKT1 tumor cells was determined by using immunohistochemical and morphometric methods. The optimal cut-off level of expression on tumor cells estimated by using receiver operating characteristic (ROC) curve analysis for pSTAT3 was 68% and for pACT1 — 70%. Given these values, all patients with DLBCL were divided into groups with a high and low degree of expression of the biomarkers. As a result, 53 patients were enrolled in the pSTAT3 high expression group (≥68% tumor cells) and 47 patients to the pSTAT3 low expression group (<68% tumor cells). Spearman’s correlation coefficient was used to examine relationships. Overall survival and event-free survival were estimated by Kaplan–Meier curves. The log-rank test was used for groups comparison. Results. The five-year overall survival rate in the pSTAT3 high expression group was 55% versus 87% in the low expression group, p=0.015. A significant difference was found in the assessment of event-free survival: 43% for the group of pSTAT3 high expression, 66% for the group of low expression, p=0.011. A statistically significant value of a high level of pACT1 expression was revealed for 5-year overall and event-free survival (p <0.001 and p=0.003). Overall survival rate was 81% for the pACT1 low expression group and 43% for the high expression group while event-free survival rate was 64 and 41%, respectively. Also, patients with рАКТ1+/рSTAT3+ (high level) co-expression had extremely low rates of overall and event-free survival rates compared with the рАКТ1–/рSTAT3– (low level) group (p=0.001; p <0.001). Conclusion. The pSTAT3 and pAKT1 biomarkers can be used as additional prognosis criteria for diffuse large B-cell lymphoma.
{"title":"Association of expression of pSTAT3, pAKT1 with the survival of patients with diffuse large B-cell lymphoma","authors":"E. Vaneeva, Ванеева Елена Викторовна, V. Rosin, Росин Виталий Анатольевич, D. Dyakonov, Дьяконов Дмитрий Андреевич, S. Samarina, Самарина Светлана Валерьевна","doi":"10.17816/kmj2020-501","DOIUrl":"https://doi.org/10.17816/kmj2020-501","url":null,"abstract":"Aim. To assess the relationship between isolated and combined expression of pSTAT3, pACT1 in tumor cells with the survival of patients with diffuse large B-cell lymphoma (DLBCL). Methods. The study included 100 patients with the first diagnosed diffuse large B-cell lymphoma, observed in the institute's clinic between 2010 and 2018 who received standard first-line R-CHOP (rituximab + cyclophosphamide, doxorubicin, vincristine and prednisone) chemotherapy. The relative number of expressing pSTAT3 and pAKT1 tumor cells was determined by using immunohistochemical and morphometric methods. The optimal cut-off level of expression on tumor cells estimated by using receiver operating characteristic (ROC) curve analysis for pSTAT3 was 68% and for pACT1 — 70%. Given these values, all patients with DLBCL were divided into groups with a high and low degree of expression of the biomarkers. As a result, 53 patients were enrolled in the pSTAT3 high expression group (≥68% tumor cells) and 47 patients to the pSTAT3 low expression group (<68% tumor cells). Spearman’s correlation coefficient was used to examine relationships. Overall survival and event-free survival were estimated by Kaplan–Meier curves. The log-rank test was used for groups comparison. Results. The five-year overall survival rate in the pSTAT3 high expression group was 55% versus 87% in the low expression group, p=0.015. A significant difference was found in the assessment of event-free survival: 43% for the group of pSTAT3 high expression, 66% for the group of low expression, p=0.011. A statistically significant value of a high level of pACT1 expression was revealed for 5-year overall and event-free survival (p <0.001 and p=0.003). Overall survival rate was 81% for the pACT1 low expression group and 43% for the high expression group while event-free survival rate was 64 and 41%, respectively. Also, patients with рАКТ1+/рSTAT3+ (high level) co-expression had extremely low rates of overall and event-free survival rates compared with the рАКТ1–/рSTAT3– (low level) group (p=0.001; p <0.001). Conclusion. The pSTAT3 and pAKT1 biomarkers can be used as additional prognosis criteria for diffuse large B-cell lymphoma.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90097851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. A. Knni, O. Radchenko, A. R. Urazmanov, L. A. Balabanova
Aim. To analyze the current state of the sanatoriums-health resorts system of the Republic of Tatarstan and identify the demand by the population of sanatoriums-health resorts services for recreation and health promotion at the regional level. Methods. To analyze the functioning of the sanatoriums-health resorts of the republic, we used data from the official website of the Federal State Statistics Service (2002–2018). We calculated the long-term mean values, standard deviation, growth rate, linear regression, the approximation coefficient in comparing the Republic of Tatarstan, Volga Federal District and the Russian Federation. The population proportion, which used the sanatoriums-health resorts services, bed provision per 10,000 population and bed turnover were calculated to assess the provision of the population with sanatorium-resort services. The study of the demand for sanatorium services by the population was carried out using a sociological survey. Results. In the comparative analysis, it was found that the Republic of Tatarstan is the region with a developed, relatively stable sanatoriums-health resorts system. The increase in the number of beds in sanatoriums (from 23.86 beds per 10,000 population in 2002 to 24.94 in 2018) and bed turnover rate (from 12.84 in 2002 to 16.59 in 2018) during the studied period indicated the continuity between curative and restorative-rehabilitation medicine. An increase in the proportion (from 3.07% in 2002 to 4.15% in 2018) with an overall increase in the number of stayed people (1.39 times) indicated the interest of the population in rehabilitation treatment and recreation in regional sanatoriums-health resorts. As a result of the survey, a “portrait” of the modern consumer of sanatoriums-health resorts was drawn up, and priorities in sanatorium choosing were determined. Conclusion. The Republic of Tatarstan belongs to the regions with a developed system of sanatoriums-health resorts and significant potential to its development, which is confirmed by the presence of natural healing factors, improved provision of sanatoriums-health resorts care and increasing demand of the population for services for rehabilitation treatment and recreation in regional sanatoriums.
{"title":"Current state and development of the sanatoriums-health resorts potential of the Republic of Tatarstan","authors":"Y. A. Knni, O. Radchenko, A. R. Urazmanov, L. A. Balabanova","doi":"10.17816/kmj2020-561","DOIUrl":"https://doi.org/10.17816/kmj2020-561","url":null,"abstract":"Aim. To analyze the current state of the sanatoriums-health resorts system of the Republic of Tatarstan and identify the demand by the population of sanatoriums-health resorts services for recreation and health promotion at the regional level. Methods. To analyze the functioning of the sanatoriums-health resorts of the republic, we used data from the official website of the Federal State Statistics Service (2002–2018). We calculated the long-term mean values, standard deviation, growth rate, linear regression, the approximation coefficient in comparing the Republic of Tatarstan, Volga Federal District and the Russian Federation. The population proportion, which used the sanatoriums-health resorts services, bed provision per 10,000 population and bed turnover were calculated to assess the provision of the population with sanatorium-resort services. The study of the demand for sanatorium services by the population was carried out using a sociological survey. Results. In the comparative analysis, it was found that the Republic of Tatarstan is the region with a developed, relatively stable sanatoriums-health resorts system. The increase in the number of beds in sanatoriums (from 23.86 beds per 10,000 population in 2002 to 24.94 in 2018) and bed turnover rate (from 12.84 in 2002 to 16.59 in 2018) during the studied period indicated the continuity between curative and restorative-rehabilitation medicine. An increase in the proportion (from 3.07% in 2002 to 4.15% in 2018) with an overall increase in the number of stayed people (1.39 times) indicated the interest of the population in rehabilitation treatment and recreation in regional sanatoriums-health resorts. As a result of the survey, a “portrait” of the modern consumer of sanatoriums-health resorts was drawn up, and priorities in sanatorium choosing were determined. Conclusion. The Republic of Tatarstan belongs to the regions with a developed system of sanatoriums-health resorts and significant potential to its development, which is confirmed by the presence of natural healing factors, improved provision of sanatoriums-health resorts care and increasing demand of the population for services for rehabilitation treatment and recreation in regional sanatoriums.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73817551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Isaeva, Исаева Гузель Шавхатовна, M. V. Stremoukhova, Стремоухова Мария Валерьевна, I. Bazikov, Базиков Игорь Александрович, I. V. Kartashev, Карташев Игорь Владимирович, A. Kartashev, Карташев Андрей Владимирович
The article was prepared for the 130th anniversary of the Head of the Department of Microbiology of Kazan State Medical University, Professor Rudolf Robertovich Geltzer. The milestones of R.R. Geltzer’s biography, and highlights the historical aspects of the formation of the Kazan school of microbiologists, which he represented are shown. The paper was prepared using primary sources — scientific articles and monographs published in the period of his activity, memories of colleagues from the Stavropol State Medical University, archival materials of the Department of Microbiology named after V.M. Aristovsky of Kazan State Medical University, as well as the National Archives of the Republic of Tatarstan. The main scientific achievements of R.R. Geltzer and his scientific contribution to the development of microbiology as one of the founders of the teaching of spirochetosis are described. The description of experimental studies on the development of culture medium for the isolation of Treponema pallidum, the search methods for diagnosing syphilis and other aspects of the scientific activity of R.R. Geltzer and his students is presented.
这篇文章是为喀山国立医科大学微生物系主任鲁道夫·罗伯特托维奇·格尔策教授建校130周年而写的。R.R.盖尔策传记的里程碑，并突出了喀山微生物学家学校形成的历史方面，他所代表的显示。这篇论文的主要资料来源是:他在活动期间发表的科学论文和专著、斯塔夫罗波尔国立医科大学同事的回忆、喀山国立医科大学以V.M.阿里斯托夫斯基命名的微生物系的档案资料以及鞑靼斯坦共和国国家档案馆。介绍了R.R. Geltzer的主要科学成就和他作为螺旋体病教学的创始人之一对微生物学发展的科学贡献。介绍了dr . r . Geltzer和他的学生在开发梅毒螺旋体分离培养基、寻找诊断梅毒的方法等方面的科学活动的实验研究。
{"title":"The pride of the Kazan school of microbiology is Professor Rudolf Robertovich Geltzer and his students (on the 130th anniversary)","authors":"G. Isaeva, Исаева Гузель Шавхатовна, M. V. Stremoukhova, Стремоухова Мария Валерьевна, I. Bazikov, Базиков Игорь Александрович, I. V. Kartashev, Карташев Игорь Владимирович, A. Kartashev, Карташев Андрей Владимирович","doi":"10.17816/kmj2020-463","DOIUrl":"https://doi.org/10.17816/kmj2020-463","url":null,"abstract":"The article was prepared for the 130th anniversary of the Head of the Department of Microbiology of Kazan State Medical University, Professor Rudolf Robertovich Geltzer. The milestones of R.R. Geltzer’s biography, and highlights the historical aspects of the formation of the Kazan school of microbiologists, which he represented are shown. The paper was prepared using primary sources — scientific articles and monographs published in the period of his activity, memories of colleagues from the Stavropol State Medical University, archival materials of the Department of Microbiology named after V.M. Aristovsky of Kazan State Medical University, as well as the National Archives of the Republic of Tatarstan. The main scientific achievements of R.R. Geltzer and his scientific contribution to the development of microbiology as one of the founders of the teaching of spirochetosis are described. The description of experimental studies on the development of culture medium for the isolation of Treponema pallidum, the search methods for diagnosing syphilis and other aspects of the scientific activity of R.R. Geltzer and his students is presented.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75617670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Юлия Валерьевна Скибо, Алия Ринатовна Фатхуллина, Булат Рафисович Ибрагимов, Сергей Николаевич Абрамов, Резида Ринатовна Исмагилова, Эльнара Маулетовна Биктагирова, Изабелла Александровна Андрианова, Аделя Наилевна Максудова, Зинаида Ивановна Абрамова, Y. V. Skibo, A. R. Fathullina, B. R. Ibragimov, S. N. Abramov, R. Ismagilova, E. M. Biktagirova, I. Andrianova, A. N. Maksudova, Z. Abramova
Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus. Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann–Whitney test. Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found. Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.
{"title":"Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus","authors":"Юлия Валерьевна Скибо, Алия Ринатовна Фатхуллина, Булат Рафисович Ибрагимов, Сергей Николаевич Абрамов, Резида Ринатовна Исмагилова, Эльнара Маулетовна Биктагирова, Изабелла Александровна Андрианова, Аделя Наилевна Максудова, Зинаида Ивановна Абрамова, Y. V. Skibo, A. R. Fathullina, B. R. Ibragimov, S. N. Abramov, R. Ismagilova, E. M. Biktagirova, I. Andrianova, A. N. Maksudova, Z. Abramova","doi":"10.17816/kmj2020-347","DOIUrl":"https://doi.org/10.17816/kmj2020-347","url":null,"abstract":"Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus. Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann–Whitney test. Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found. Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85578549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. M. Vahitov, Вахитов Хаким Муратович, A. Makhmutova, Махмутова Алина Гусмановна, L. M. Ziyatdinova, Зиятдинова Лилия Масгутовна, M. S. Pospelov, Поспелов Михаил Сергеевич
This article describes up-to-date information about aetiology and pathogenesis of course of herpetic eczema. The authors highlighted new approaches to diagnosis and therapy of Kaposi varicelliform eruption in children of early age. It was described the results of own observations of the child in the first year of life with Kaposi varicelliform eruption and experience of application-sorption therapy in the treatment of this disease. The high efficiency of application-sorption therapy in skin lesions caused by combination of infection and allergy in children was noted. The clinical case examined allowed to assess the full complexity of differential diagnostic approaches in verification of eczema herpeticum. For a wide range of paediatricians and surgeons, algorithms for diagnosis and therapeutic tactics were described in the case of Kaposi varicelliform eruption.
{"title":"Eczema herpeticum in a child in the first year of life: a clinical case report","authors":"H. M. Vahitov, Вахитов Хаким Муратович, A. Makhmutova, Махмутова Алина Гусмановна, L. M. Ziyatdinova, Зиятдинова Лилия Масгутовна, M. S. Pospelov, Поспелов Михаил Сергеевич","doi":"10.17816/kmj2020-426","DOIUrl":"https://doi.org/10.17816/kmj2020-426","url":null,"abstract":"This article describes up-to-date information about aetiology and pathogenesis of course of herpetic eczema. The authors highlighted new approaches to diagnosis and therapy of Kaposi varicelliform eruption in children of early age. It was described the results of own observations of the child in the first year of life with Kaposi varicelliform eruption and experience of application-sorption therapy in the treatment of this disease. The high efficiency of application-sorption therapy in skin lesions caused by combination of infection and allergy in children was noted. The clinical case examined allowed to assess the full complexity of differential diagnostic approaches in verification of eczema herpeticum. For a wide range of paediatricians and surgeons, algorithms for diagnosis and therapeutic tactics were described in the case of Kaposi varicelliform eruption.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88653177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Bayazitova, Баязитова Лира Табрисовна, O. Tupkina, Тюпкина Ольга Феликсовна, T. A. Chazova, Чазова Татьяна Александровна, N. S. Konyshev, Конышев Никита Сергеевич, K. N. Syuzev, Сюзев Кирилл Николаевич, G. Isaeva, Исаева Гузель Шавхатовна
Aim. To study the nature of microbiota and estimating the susceptibility to antibiotics and bacteriophages of conditionally pathogenic microflora of the nasopharynx in children-pneumococcal carriers with recurrent respiratory infections. Methods. Researching microflora was conducted in 182 pneumococcal carriers receiving help in Kazan Scientific and Research Institute of Epidemiology and Microbiology. Microbial identification, testing of susceptibility to antibiotics and bacteriophages was carried out following the regulatory documentation. Bacterial isolates were confirmed by mass spectrometry. The phage titer was determined by the method of agar layers according to Grazia. Results. Nasopharyngeal S. pneumoniae species was presented by Staphylococcus spp., Moraxella spp., Haemophilus spp., Corynebacterium spp., Klebsiella spp and Candida spp. The antimicrobial resistance profiles of Streptococcus pneumoniae: resistant to oxacillin was detected in 20.7% of strains, to erythromycin in 45.9%, to clindamycin in 20%, to trimethoprim-sulfamethoxazole in 18.4%. 19.6% of isolates were multidrug-resistant (MDR, resistant to 3 or more antimicrobial agents). Phage susceptibility test of S. pneumoniaе showed that 97.2% of isolates were resistant to streptococcal bacteriophage, 75% to pyobacteriophage. All antibiotic-resistant strains remained susceptible to Streptococcus phages. The phage titer of Klebsiella in agreement with Grazia method of Kl. pneumoniae ranged from 9×10–6 to 5×10–5 PFU/mL. The ranking results of activities of antistaphylococcal antibiotics (effectiveness descending): fusidic acid > mupirocin > chloramphenicol > cyprofloxacin erythromycin. Conclusion. Nasopharyngeal microbiota of pneumococci carriers children is represented by a variable polymicrobial association; nasopharyngeal strains are effectively lysed by bacteriophages; mono- and polyvalent bacteriophages can be used as an alternative to antibacterial treatment in Streptococcus pneumoniae carriers children with recurrent respiratory infections.
{"title":"Phage sensitivity profiles of a nasopharyngeal opportunistic pathogen in Streptococcus pneumoniae carrier children with recurrent respiratory infections","authors":"L. Bayazitova, Баязитова Лира Табрисовна, O. Tupkina, Тюпкина Ольга Феликсовна, T. A. Chazova, Чазова Татьяна Александровна, N. S. Konyshev, Конышев Никита Сергеевич, K. N. Syuzev, Сюзев Кирилл Николаевич, G. Isaeva, Исаева Гузель Шавхатовна","doi":"10.17816/kmj2020-330","DOIUrl":"https://doi.org/10.17816/kmj2020-330","url":null,"abstract":"Aim. To study the nature of microbiota and estimating the susceptibility to antibiotics and bacteriophages of conditionally pathogenic microflora of the nasopharynx in children-pneumococcal carriers with recurrent respiratory infections. Methods. Researching microflora was conducted in 182 pneumococcal carriers receiving help in Kazan Scientific and Research Institute of Epidemiology and Microbiology. Microbial identification, testing of susceptibility to antibiotics and bacteriophages was carried out following the regulatory documentation. Bacterial isolates were confirmed by mass spectrometry. The phage titer was determined by the method of agar layers according to Grazia. Results. Nasopharyngeal S. pneumoniae species was presented by Staphylococcus spp., Moraxella spp., Haemophilus spp., Corynebacterium spp., Klebsiella spp and Candida spp. The antimicrobial resistance profiles of Streptococcus pneumoniae: resistant to oxacillin was detected in 20.7% of strains, to erythromycin in 45.9%, to clindamycin in 20%, to trimethoprim-sulfamethoxazole in 18.4%. 19.6% of isolates were multidrug-resistant (MDR, resistant to 3 or more antimicrobial agents). Phage susceptibility test of S. pneumoniaе showed that 97.2% of isolates were resistant to streptococcal bacteriophage, 75% to pyobacteriophage. All antibiotic-resistant strains remained susceptible to Streptococcus phages. The phage titer of Klebsiella in agreement with Grazia method of Kl. pneumoniae ranged from 9×10–6 to 5×10–5 PFU/mL. The ranking results of activities of antistaphylococcal antibiotics (effectiveness descending): fusidic acid > mupirocin > chloramphenicol > cyprofloxacin erythromycin. Conclusion. Nasopharyngeal microbiota of pneumococci carriers children is represented by a variable polymicrobial association; nasopharyngeal strains are effectively lysed by bacteriophages; mono- and polyvalent bacteriophages can be used as an alternative to antibacterial treatment in Streptococcus pneumoniae carriers children with recurrent respiratory infections.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73448112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. B. Tashtanbekova, E. A. Chuenkova, A. Evstratov, L. Ziganshina
Aim. To study the use of drugs for pain management for cesarean section and their cost on time and the first day after surgery. Methods. A retrospective analysis of 117 anesthesia cards and childbirth histories of women after a cesarean section was performed. We analyzed drug therapy aimed at reducing pain during 1 day after cesarean section and performed a comparative analysis of the cost of drugs used in spinal and epidural anesthesia. Results. Regional methods of anesthesia, epidural and spinal, were used in 95% of all cases. Spinal anesthesia was performed in 77 women, epidural — in 34 women. The frequency of prescribing opioid analgesics was higher with spinal anesthesia compared with epidural: trimeperidine (intramuscular) was used in 62 (83%) of 77 patients for spinal anesthesia and 1 (3%) of 34 for epidural anesthesia (p <0.05). There were no differences in the use of ketoprofen in the postoperative period with epidural and spinal anesthesia. The total cost of medicines used to control pain during and on the 1st day after surgery, with epidural anesthesia, was almost 10 times higher than that of spinal anesthesia: 938 and 98 rubles, respectively. Conclusion. To control pain during cesarean section, in addition to local anesthetics, trimeperidine was used more often with spinal than epidural anesthesia; in the postoperative period, ketoprofen and trimeperidine were used with the equal frequency with greater use of ropivacaine with epidural anesthesia through a stored catheter; this has caused a higher cost of pain management during and in the first day after cesarean section with epidural anesthesia.
{"title":"Use and costs of pain management in cesarian section","authors":"C. B. Tashtanbekova, E. A. Chuenkova, A. Evstratov, L. Ziganshina","doi":"10.17816/kmj2020-418","DOIUrl":"https://doi.org/10.17816/kmj2020-418","url":null,"abstract":"Aim. To study the use of drugs for pain management for cesarean section and their cost on time and the first day after surgery. Methods. A retrospective analysis of 117 anesthesia cards and childbirth histories of women after a cesarean section was performed. We analyzed drug therapy aimed at reducing pain during 1 day after cesarean section and performed a comparative analysis of the cost of drugs used in spinal and epidural anesthesia. Results. Regional methods of anesthesia, epidural and spinal, were used in 95% of all cases. Spinal anesthesia was performed in 77 women, epidural — in 34 women. The frequency of prescribing opioid analgesics was higher with spinal anesthesia compared with epidural: trimeperidine (intramuscular) was used in 62 (83%) of 77 patients for spinal anesthesia and 1 (3%) of 34 for epidural anesthesia (p <0.05). There were no differences in the use of ketoprofen in the postoperative period with epidural and spinal anesthesia. The total cost of medicines used to control pain during and on the 1st day after surgery, with epidural anesthesia, was almost 10 times higher than that of spinal anesthesia: 938 and 98 rubles, respectively. Conclusion. To control pain during cesarean section, in addition to local anesthetics, trimeperidine was used more often with spinal than epidural anesthesia; in the postoperative period, ketoprofen and trimeperidine were used with the equal frequency with greater use of ropivacaine with epidural anesthesia through a stored catheter; this has caused a higher cost of pain management during and in the first day after cesarean section with epidural anesthesia.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91286520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. A. Atakishizade, Атакишизаде Садраддин Абдулла оглы
Aim. To study of the antibiotic resistance of S. aureus strains isolated from nosocomial infections (pneumonia, surgical site infections and sepsis) in a multidisciplinary surgical clinic. Methods. Microbiological testing of sputum in 41 patients with pneumonia, of samples (wound, abscess, drainage) obtained from 40 patients with surgical site infections (SSI) and of blood from 46 patients with signs of sepsis was performed. The obtaining cultures were identified by conventional methods (including morphological, cultural, biochemical features, etc.). Disc diffusion method was used to detect methicillin-resistant S. aureus (MRSA) strains. Inducible clindamycin resistance (ICR) of S. aureus strains was determined by double disk approximation test (D-test). Results. Methicillin-resistant S. aureus was found in 14.3% (2 of 14) of the patients with surgical site infections, in the sputum 27.3% (3 of 11) of the patients with pneumonia, and in the blood 50.0% (7 of 14) of the patients with sepsis (p >0.05). The rate of inducible clindamycin resistance of isolated S. aureus strains in patients with surgical site infections (2 of 14 cases, 14.3%) and with pneumonia (2 of 11 cases, 18.2%) did not statistically significant difference with the rate of methicillin resistance (p >0.05). However the rate was significantly lower septic infections — 7.1% and 50.0% respectively (p=0.0328). Conclusion. Among S. aureus strains isolated from nosocomial infections, the rate of methicillin-resistant S. aureus had not depended on the type of nosocomial infection; the rate of inducible clindamycin resistance in septic infections was lower than resistance to methicillin.
{"title":"Peculiarities of antibiotic resistance of Staphylococcus aureus strains isolated from nosocomial infections","authors":"S. A. Atakishizade, Атакишизаде Садраддин Абдулла оглы","doi":"10.17816/kmj2020-325","DOIUrl":"https://doi.org/10.17816/kmj2020-325","url":null,"abstract":"Aim. To study of the antibiotic resistance of S. aureus strains isolated from nosocomial infections (pneumonia, surgical site infections and sepsis) in a multidisciplinary surgical clinic. Methods. Microbiological testing of sputum in 41 patients with pneumonia, of samples (wound, abscess, drainage) obtained from 40 patients with surgical site infections (SSI) and of blood from 46 patients with signs of sepsis was performed. The obtaining cultures were identified by conventional methods (including morphological, cultural, biochemical features, etc.). Disc diffusion method was used to detect methicillin-resistant S. aureus (MRSA) strains. Inducible clindamycin resistance (ICR) of S. aureus strains was determined by double disk approximation test (D-test). Results. Methicillin-resistant S. aureus was found in 14.3% (2 of 14) of the patients with surgical site infections, in the sputum 27.3% (3 of 11) of the patients with pneumonia, and in the blood 50.0% (7 of 14) of the patients with sepsis (p >0.05). The rate of inducible clindamycin resistance of isolated S. aureus strains in patients with surgical site infections (2 of 14 cases, 14.3%) and with pneumonia (2 of 11 cases, 18.2%) did not statistically significant difference with the rate of methicillin resistance (p >0.05). However the rate was significantly lower septic infections — 7.1% and 50.0% respectively (p=0.0328). Conclusion. Among S. aureus strains isolated from nosocomial infections, the rate of methicillin-resistant S. aureus had not depended on the type of nosocomial infection; the rate of inducible clindamycin resistance in septic infections was lower than resistance to methicillin.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83420633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Abramov, R. Goloshchapov-Aksenov, D. Kicha, O. Rukodayny
Aim. To develop an algorithm for primary specialized cardiovascular care with a priority of endovascular strategy. Methods. The study was conducted in 2018–2019 based on the Central Clinical Hospital “Russian Railways-Medicine” and 14 polyclinics in the regions of the Russian Federation. The subject of the study is cardiovascular surgeons (n=2), possessing the skills of endovascular care. The object of the study was patients (n=1018) attended regional polyclinics of the Russian Federation. Patients were divided into two groups: group A consisting of 673 patients with clinically significant atherosclerosis of the coronary, brachiocephalic and peripheral arteries and abdominal aortic aneurysm; group B consisting of 345 patients with chronic lower limb ischemia that does not require surgical treatment. The average age of patients in group A was 69±6.1 years, in group B — 63±7.2 years. There were 467 men in group A (69.4%), and 339 in group B (98.3%). An organizational and technological algorithm was developed to improve the primary specialized cardiovascular care. The results were assessed by the presence of outcomes (heart attack, stroke, bleeding, death), the availability of endovascular care and patient survival follow up 12 and 24 months. A content analysis of scientific publications on the issue under study has been performed. Results. An organizational and technological algorithm of primary specialized cardiovascular care has been developed, including the activities of the cardiovascular surgeon, who has the skills of endovascular care and a nurse in an outpatient clinic. The implementation of the algorithm ensured continuity, 100% availability, safety and quality of cardiovascular care using endovascular technology. Outcomes are not registered in both groups. Both patient groups showed 100% one and two-year survival. Conclusion. The developed algorithm of primary specialized cardiovascular care has provided high quality healthcare.
{"title":"Organizational and technological algorithm of primary specialized health care at cardiovascular diseases","authors":"A. Abramov, R. Goloshchapov-Aksenov, D. Kicha, O. Rukodayny","doi":"10.17816/kmj2020-394","DOIUrl":"https://doi.org/10.17816/kmj2020-394","url":null,"abstract":"Aim. To develop an algorithm for primary specialized cardiovascular care with a priority of endovascular strategy. Methods. The study was conducted in 2018–2019 based on the Central Clinical Hospital “Russian Railways-Medicine” and 14 polyclinics in the regions of the Russian Federation. The subject of the study is cardiovascular surgeons (n=2), possessing the skills of endovascular care. The object of the study was patients (n=1018) attended regional polyclinics of the Russian Federation. Patients were divided into two groups: group A consisting of 673 patients with clinically significant atherosclerosis of the coronary, brachiocephalic and peripheral arteries and abdominal aortic aneurysm; group B consisting of 345 patients with chronic lower limb ischemia that does not require surgical treatment. The average age of patients in group A was 69±6.1 years, in group B — 63±7.2 years. There were 467 men in group A (69.4%), and 339 in group B (98.3%). An organizational and technological algorithm was developed to improve the primary specialized cardiovascular care. The results were assessed by the presence of outcomes (heart attack, stroke, bleeding, death), the availability of endovascular care and patient survival follow up 12 and 24 months. A content analysis of scientific publications on the issue under study has been performed. Results. An organizational and technological algorithm of primary specialized cardiovascular care has been developed, including the activities of the cardiovascular surgeon, who has the skills of endovascular care and a nurse in an outpatient clinic. The implementation of the algorithm ensured continuity, 100% availability, safety and quality of cardiovascular care using endovascular technology. Outcomes are not registered in both groups. Both patient groups showed 100% one and two-year survival. Conclusion. The developed algorithm of primary specialized cardiovascular care has provided high quality healthcare.","PeriodicalId":17798,"journal":{"name":"Kazanskiy meditsinskiy zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77199627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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