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System for the evaluation of malting quality of wheat varieties 小麦品种麦芽品质评价体系
Pub Date : 2020-01-22 DOI: 10.18832/kp2020.66.232
V. Psota, M. Musilová
A new system for the calculation of malting quality of wheat varieties was designed. The malting parameters affected by a variety more than by a year or a site were used in the system. These parameters include wort viscosity, soluble nitrogen content, free amino nitrogen content, diastatic power, final attenuation of wort, and extract of malt. The system was used to assess the malting quality in 38 wheat varieties. The point value of each parameter was calculated using the regression equations, and the total value of the malting quality of wheat was determined by the modified “superiority measure” method. The results of the assessment of the wheat malting quality showed considerable differences between the varieties. Several varieties exhibited a more pronounced tendency to accumulate nitrogenous substances in grain. Some varieties, even with a slightly higher content of nitrogenous substances, provided above average malt quality. The set included the varieties that exhibited the optimal content of nitrogenous substances in grain, yet they did not give malt of good quality. Spring wheat varieties Alondra, Anabel, and Seance achieved significantly above average malting quality. Dagmar, Evina, Sultan, Rumor, Sailor, and Brokat exhibited slightly above average malting quality. The proposed system for monitoring malting quality of wheat varieties can help find the varieties suitable for the production of quality wheat malt.
设计了一种新的小麦品种麦芽品质计算系统。采用受品种影响大于年份或地点的麦芽酿造参数。这些参数包括麦汁粘度、可溶性氮含量、游离氨基氮含量、消散力、麦汁最终衰减和麦芽提取物。应用该系统对38个小麦品种的麦芽品质进行了评价。利用回归方程计算各参数的点值,采用改进的“优势测度法”确定小麦麦芽品质的总值。小麦麦芽品质评价结果显示,品种间存在较大差异。有几个品种表现出更明显的籽粒氮素积累趋势。有些品种,甚至含氮物质含量略高,提供高于平均水平的麦芽品质。这一组包括籽粒含氮物质含量最佳但品质不佳的品种。春小麦品种Alondra、Anabel和Seance的麦芽质量明显高于平均水平。Dagmar, Evina, Sultan, Rumor, Sailor和Brokat的麦芽质量略高于平均水平。所建立的小麦品种麦芽品质监测系统,有助于找到适合生产优质小麦的品种。
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引用次数: 6
Yeast need not be used only in beer production. I. The carotenoid astaxanthin produced by yeast. 酵母不需要只用于啤酒生产。由酵母产生的类胡萝卜素虾青素
Pub Date : 2020-01-22 DOI: 10.18832/kp2020.66.208
T. Řezanka, A. Palyzová
Carotenoids are produced by photosynthesizing organisms, i.e. plants and algae, as well as some bacteria, protists and fungi. Their role lies mainly in the function of complementary photosynthetic pigments and antioxidants. Carotenoids are distinguished according to their structure into carotenoids of the hydrocarbon type, e.g. α-, β- and γ-carotene and oxygen derivatives – xanthophylls, among which astaxanthin, which excels in its antioxidant and nutraceutical properties, is the most important. It is used as a dietary supplement both in human nutrition and in the aquaculture and poultry industry, as a nutritional supplement in salmonid and hen farming. Many works have focused on the search for natural sources of astaxanthin as an alternative to synthetic astaxanthin. In nature, very few astaxanthin producing organisms are known in non-minor amounts, with green alga Haematococcus pluvialis and the yeast Xanthophyllomyces dendrorhous (anamorph Phaffia rhodozyma) being the most promising microorganisms for the biotechnological production of this xanthophyll. In this review we focus on the biosynthesis and carotenogenesis including the regulation mechanisms, generation of hyperproducing mutants by random mutagenesis and, last but not least, also metabolic engineering (genetic techniques). Attention is also paid to the optimization of fermentation conditions.
类胡萝卜素是由光合作用生物,即植物和藻类,以及一些细菌、原生生物和真菌产生的。它们的作用主要在于互补光合色素和抗氧化剂的功能。类胡萝卜素根据其结构可分为烃类类胡萝卜素,如α-、β-、γ-胡萝卜素和氧衍生物-叶黄素,其中以虾青素最为重要,具有抗氧化和营养保健的特性。它被用作人类营养的膳食补充剂,在水产养殖业和家禽业,作为鲑鱼和母鸡养殖的营养补充剂。许多工作都集中在寻找虾青素的天然来源,作为合成虾青素的替代品。在自然界中,已知的产生虾青素的生物很少,其中绿藻雨生红球菌(Haematococcus pluvialis)和酵母菌(anamorph Phaffia rhodozyma)是最有希望利用生物技术生产这种叶黄素的微生物。本文综述了胡萝卜素的生物合成和生成,包括调控机制、随机诱变高产突变体的产生以及代谢工程(基因技术)。发酵条件的优化也引起了人们的注意。
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引用次数: 0
A comparison of sensory evaluation and an electronic nose assay in the assessment of aroma in dry hopped beers 感官评价与电子鼻法在干啤酒花香气评价中的比较
Pub Date : 2020-01-20 DOI: 10.18832/kp2020.66.224
J. Štefániková, V. Nagyová, Matej Hynšt, Dominika Kudláková, J. Árvay, Štefan Dráb
The aim of this work was to compare sensory evaluation and electronic nose technology used to assess aromas in dry hopped beers. An electronic nose based on gas chromatography was used for the first time. Hops varieties Amarillo, Cascade, Chinook, Kazbek and Mandarina Bavaria were used for the production of dry hopped beers and the Sladek variety was used for the control sample. The basic characteristics of the beers were determined, and the sensory evaluation performed by selected assessors was compared to the sensory assay using an electronic nose. Assessment of the aroma profile of dry hopped beers shows that the basic flavours of these beers, such as worty, yeasty and hoppy, were suppressed. Compared to the control sample, a significant grapefruit flavour was noted by the evaluators in Kazbek and Chinook beer samples. The most prominent determinant, compared to the control sample, was in general the citrus aroma. Based on the results of the principal component analysis, it can be concluded that there were statistically significant differences between the individual dry hopped beers and between them and the control sample with the exception of beers dry hopped with hops of the Amarillo and Cascade variety, which was also confirmed by the results of sensory evaluation (approximately the same scoring of the monitored descriptors).
这项工作的目的是比较感官评价和电子鼻技术用于评估干啤酒花的香味。首次使用了基于气相色谱的电子鼻。用阿马里洛、喀斯喀特、奇努克、卡兹别克和曼达里纳巴伐利亚啤酒花品种生产干啤酒花啤酒,用斯莱德克品种作为对照样品。确定了啤酒的基本特征,并将选定的评估人员进行的感官评估与使用电子鼻的感官分析进行比较。对干啤酒花啤酒的香气特征的评估表明,这些啤酒的基本风味,如麦芽味、酵母味和啤酒花味,被抑制了。与对照样品相比,卡兹别克和奇努克啤酒样品中有明显的葡萄柚味。与对照样品相比,最显著的决定因素通常是柑橘的香气。根据主成分分析的结果,可以得出结论,除了用阿马里洛和Cascade品种的啤酒花干跳的啤酒外,个体干跳啤酒与对照样品之间存在统计学显著差异,这也得到了感官评价结果的证实(监测描述符的得分大致相同)。
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引用次数: 3
Application of widely used fungicides does not necessarily affect grain yield, and incidence of Fusarium spp. and mycotoxins DON, HT-2 and T-2 in spring barley in northern climates 在北方气候条件下,广泛使用的杀菌剂的施用并不一定影响籽粒产量和镰刀菌及其毒素DON、HT-2和T-2在春大麦中的发病率
Pub Date : 2020-01-17 DOI: 10.18832/kp2020.66.215
E. Karron, E. Runno-Paurson, H. Lõiveke, Bulat Islamov, M. Kütt, T. Talve, E. Lauringson, Hanna Hõrak, L. Edesi, Ü. Niinemets
Fungicides are widely used to reduce Fusarium infections and grain contamination by mycotoxins and increase the yield in cereals, but the efficacy of fungicide treatments in varying climates has not been systematically explored. Field experiments with Estonian spring barley (Hordeum vulgare L.) cv. ‘Maali’ were carried out in three successive years 2012–2014 with strongly varying weather conditions to study the effects of three fungicides, Folicur (active ingredient tebuconazole), Falcon Forte (prothioconazole, tebuconazole, spiroxamine) and Archer Top (fenpropidin, propiconazole), on the yield, incidence of Fusarium spp. and on the contamination of grain with mycotoxins DON, HT-2 and T-2. The fungicides were sprayed once a year at spring barley flowering time. The weather conditions during the three years of study were extremely different. The content of cycotoxin DON, HT2 and T2 was low. The spraying with fungicides had not a clear effect on the barley yield and 1 000 kernel weight, and the study year was primarily the main factor that affected barley yield (p<0.05) and 1 000 kernel weight (p<0.05). The impact of year together with fungicide treatment had a significant effect on the incidence of Fusarium spp. (p<0.05) and on the incidence of mycotoxin DON in barley kernels (p<0.001), but did not have a clear effect on the incidence of mycotoxins HT2 and T2.
杀菌剂被广泛用于减少镰刀菌感染和真菌毒素对谷物的污染,提高谷物产量,但在不同气候条件下,杀菌剂处理的效果尚未得到系统的探讨。爱沙尼亚春大麦(Hordeum vulgare L.)田间试验。“Maali”试验于2012-2014年连续三年在气候条件强烈变化的情况下进行,研究了三种杀菌剂Folicur(有效成分tebuconazole)、Falcon Forte(原硫康唑、tebuconazole、spiroxamine)和Archer Top(苯丙定、丙环唑)对赤霉病的产量、发病率以及真菌毒素DON、HT-2和T-2对粮食污染的影响。在春大麦开花期每年喷洒一次杀菌剂。在这三年的研究中,天气条件非常不同。环毒素DON、HT2、T2含量较低。喷施杀菌剂对大麦产量和千粒重影响不明显,研究年份是影响大麦产量和千粒重的主要因素(p<0.05)。年份和杀菌剂处理对大麦籽粒镰刀菌的发病率有显著影响(p<0.05),对真菌毒素DON的发病率有显著影响(p<0.001),但对真菌毒素HT2和T2的发病率无明显影响。
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引用次数: 6
Phthalates in beverages - A review 饮料中的邻苯二甲酸盐综述
Pub Date : 2020-01-03 DOI: 10.18832/kp2019.66.264
Tomáš Horák, J. Olšovská
Phthalates as esters of phthalic acid and aliphatic alcohol are environmental contaminants which are commonly observed due to their widespread use as plasticizers. Their chemical and physical properties allow them to migrate into the environment and also into foods and beverages easily. The objective of this paper is a summarized overview of phthalate properties, their impact on human health, their content in beverages, especially in beer, including the ways of contamination, and last but not least also legislative regulations.
邻苯二甲酸酯作为邻苯二甲酸酯和脂肪醇是环境污染物,由于它们广泛用作增塑剂而被普遍观察到。它们的化学和物理特性使它们很容易迁移到环境中,也很容易进入食品和饮料中。本文的目的是概述邻苯二甲酸酯的性质,其对人体健康的影响,其在饮料,特别是啤酒中的含量,包括污染的方式,最后但并非最不重要的立法法规。
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引用次数: 1
Proteomic Analysis of Wort with Focus on Potential Differentiation of Malting Barley Cultivars 麦汁蛋白质组学分析——以麦芽品种分化潜力为重点
Pub Date : 2019-12-16 DOI: 10.18832/kp2019.65.186
M. Scigelová, M. Dušek, Vladimíra Jandovská, J. Olšovská
We analyzed the profile of malt proteins in boiled wort preparations using liquid chromatography coupled to high resolution mass spectrometry. Proteins detected in 9 different malt barley cultivars were identified. We probed proteins for post-translational modifications, namely glycation. Differential analysis of the wort proteomes provided insight into the effect of malting barley cultivar as well as location of cultivation. The results suggest that proteome analysis of wort samples could allow for identification/classification of malting barley cultivars used in wort production.
采用液相色谱-高分辨质谱联用技术对煮沸麦汁制剂中的麦芽蛋白进行了分析。对9个不同麦芽大麦品种的蛋白质进行了鉴定。我们研究了蛋白质的翻译后修饰,即糖基化。麦芽汁蛋白质组的差异分析有助于了解麦芽品种的影响以及栽培地点。结果表明,对麦芽汁样品进行蛋白质组学分析可以对用于生产麦芽汁的麦芽品种进行鉴定和分类。
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引用次数: 0
Seed germination promoting chemical compounds and their potential use in the malting industry 促进种子萌发的化合物及其在麦芽工业中的潜在应用
Pub Date : 2019-12-11 DOI: 10.18832/kp2020.66.201
Hana Dufková
Malting of barley grains is a procedure known for centuries, however, the technology of malt production is constantly being studied and optimized. The optimizations comprise different conditions of barley steeping, as well as using different additives to improve seed germination. Among the most famous group of seed germination regulators, gibberellins, a broad spectrum of diverse chemical substances also have stimulatory effects on seed germination. This review summarizes what is known about multiple barley seed germination promoting compounds, as well as other chemical/hormonal seed germination regulators described in other plant species. The goal is to provide an impulse for testing new stimulatory substances with potential applications in malting process optimization.
大麦谷物的麦芽酿造是一种已知了几个世纪的工艺,然而,麦芽生产的技术正在不断地被研究和优化。优化包括不同的大麦浸泡条件,以及使用不同的添加剂来提高种子发芽。在最著名的一类种子萌发调节剂赤霉素中,多种多样的化学物质也对种子萌发有刺激作用。本文综述了大麦种子萌发促进化合物的研究进展,以及其他植物种子萌发调节因子的化学/激素研究进展。目的是为测试具有潜在应用于麦芽工艺优化的新刺激物质提供动力。
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引用次数: 0
How wort boiling process affects flavonoid polyphenols in beer 麦汁煮沸过程对啤酒中类黄酮多酚的影响
Pub Date : 2019-12-10 DOI: 10.18832/kp2019.65.192
A. Mikyška, M. Dušek
Beer and brewing raw materials are a source of number of polyphenol substances with potential or proven beneficial biological effects. In pilot brews of Czech pale lager (200 l) the behavior of hop-derived polyphenol substances during wort boiling process in various hopping regimes was studied. Prenylflavonoids, flavonoids and their O-glycosides were determined by liquid chromatography coupled to high resolution mass spectrometry (LC-HR / MS) with sample preparation by the QuEChERS method. The concentration of flavanols (catechin, epicatechin) in hopped wort and beer is dominated by hop raw material, when hopping with aromatic hops rich in polyphenols, 2/3 of the amount in beer is affected by hops. In contrast, the concentration of their mono-O-glucosides in beer does not depend on hopping. Flavonols quercetin, kaempferol and multifidol are found in wort and beer mainly in glycosidic form. The source of all these substances in beer is exclusively hops. Conversely, malt is the only source of myricetin and myricetin-O-glucoside in beer. Main part of hop-derived flavonoids is released during wort boiling into wort within 15-30 minutes. Splitting the hop dose or low pressure boiling technology had no significant effect on health-promoting polyphenols. Beer hopped with hops rich in flavonoids contains a relatively high amount of health-promoting polyphenols, while beer hopped with hop extract is very poor in these antioxidants.
啤酒和酿造原料是许多具有潜在或已证实的有益生物效应的多酚物质的来源。在捷克淡啤酒(200 l)中试啤酒中,研究了不同跳跃条件下啤酒花衍生多酚物质在麦汁沸腾过程中的行为。采用液相色谱-高分辨质谱联用技术(LC-HR / MS)测定丙烯基类黄酮、类黄酮及其o -糖苷,样品制备采用QuEChERS法。酒花麦芽汁和啤酒中黄烷醇(儿茶素、表儿茶素)的含量主要受啤酒花原料的影响,当用富含多酚的芳香啤酒花酿造啤酒时,啤酒中2/3的量受啤酒花的影响。相反,啤酒中它们的单- o-糖苷的浓度不依赖于跳跃。黄酮醇、槲皮素、山奈酚和多元醇主要以糖苷形式存在于麦汁和啤酒中。啤酒中所有这些物质都来自啤酒花。相反,麦芽是啤酒中杨梅素和杨梅素- o -葡萄糖苷的唯一来源。啤酒花类黄酮的主要成分是在15-30分钟的麦汁煮沸过程中释放出来的。拆分啤酒花剂量或低压煮沸工艺对促进健康的多酚含量无显著影响。用富含黄酮类化合物的啤酒花酿造的啤酒含有相对较高的促进健康的多酚,而用啤酒花提取物酿造的啤酒中这些抗氧化剂含量非常低。
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引用次数: 6
Varietal specificity of polyphenols, free phenolics and antioxidant potential in hops 啤酒花中多酚、游离酚和抗氧化潜能的品种特异性
Pub Date : 2019-11-29 DOI: 10.18832/kp2019.65.178
A. Mikyška, M. Jurkovă
Many studies have confirmed hops (Humulus lupulus L.) as the source of a number of physiologically active polyphenols, important for both brewing and potentially pharmaceutical purposes. The three-year screening of polyphenols and 23 free phenolics profile (HPLC/coulometric detection) showed a significant association of polyphenols and free phenolics with DPPH antioxidant potential and the genetically determined specificity of free phenolics composition in hops. Varieties of American origin have a different profile compared to European varieties. For the first time, the free phenolics hydroxycoumarins were detected in hops. The DPPH strongly correlated with total polyphenols and flavanoids (n = 95, r = 0.92, 0.93 respectively) and correlated negatively with alpha acids (n = 95, r = -0.73). The polyphenols and the DPPH decrease with increasing alpha acids both among varieties and within one variety. These findings can serve as a useful tool for hop selection in brewing or pharmaceutical production.
许多研究已经证实啤酒花(Humulus lupulus L.)是许多生理活性多酚的来源,对酿造和潜在的药物用途都很重要。为期三年的多酚和23种游离酚谱筛选(HPLC/库伦法检测)表明,多酚和游离酚与DPPH抗氧化能力和遗传决定的啤酒花中游离酚成分的特异性显著相关。与欧洲品种相比,美洲品种具有不同的特征。首次在啤酒花中检测到游离酚类羟基香豆素。DPPH与总多酚和黄酮呈显著正相关(n = 95, r = 0.92, 0.93),与α酸呈显著负相关(n = 95, r = -0.73)。多酚和DPPH在品种间和同一品种内均随α -酸的增加而降低。这些发现可以作为酿造或制药生产中啤酒花选择的有用工具。
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引用次数: 6
A novel method of furan determination in beer using gas chromatography with a flame ionization detector 建立了气相色谱火焰电离检测器测定啤酒中呋喃的新方法
Pub Date : 2019-11-14 DOI: 10.18832/kp2019.65.174
J. Olšovská, Tomáš Horák, K. Štěrba
Furan is considered to have a potential carcinogenic effect on human health. Its presence was found in a number of foods including beer. Thus, it is necessary to have a fast and routine method for its determination. This study describes a procedure meeting these requirements using the head-space technique associated with gas chromatography. A capillary column with the length of 60 m, inner diameter of 0.32 mm and the film thickness of 0.25 μm was used for the determination of furan. A flame-ionization detector (FID) was used for the detection. The characteristics of the procedure are presented.
呋喃被认为对人类健康有潜在的致癌作用。它存在于包括啤酒在内的许多食物中。因此,有必要建立一种快速、常规的测定方法。本研究描述了一种使用与气相色谱相关的顶空技术满足这些要求的程序。采用毛细管柱测定呋喃,柱长60 m,内径0.32 mm,膜厚0.25 μm。采用火焰电离检测器(FID)进行检测。介绍了该方法的特点。
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引用次数: 0
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Kvasny prumysl
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