A new system for the calculation of malting quality of wheat varieties was designed. The malting parameters affected by a variety more than by a year or a site were used in the system. These parameters include wort viscosity, soluble nitrogen content, free amino nitrogen content, diastatic power, final attenuation of wort, and extract of malt. The system was used to assess the malting quality in 38 wheat varieties. The point value of each parameter was calculated using the regression equations, and the total value of the malting quality of wheat was determined by the modified “superiority measure” method. The results of the assessment of the wheat malting quality showed considerable differences between the varieties. Several varieties exhibited a more pronounced tendency to accumulate nitrogenous substances in grain. Some varieties, even with a slightly higher content of nitrogenous substances, provided above average malt quality. The set included the varieties that exhibited the optimal content of nitrogenous substances in grain, yet they did not give malt of good quality. Spring wheat varieties Alondra, Anabel, and Seance achieved significantly above average malting quality. Dagmar, Evina, Sultan, Rumor, Sailor, and Brokat exhibited slightly above average malting quality. The proposed system for monitoring malting quality of wheat varieties can help find the varieties suitable for the production of quality wheat malt.
{"title":"System for the evaluation of malting quality of wheat varieties","authors":"V. Psota, M. Musilová","doi":"10.18832/kp2020.66.232","DOIUrl":"https://doi.org/10.18832/kp2020.66.232","url":null,"abstract":"A new system for the calculation of malting quality of wheat varieties was designed. The malting parameters affected by a variety more than by a year or a site were used in the system. These parameters include wort viscosity, soluble nitrogen content, free amino nitrogen content, diastatic power, final attenuation of wort, and extract of malt. The system was used to assess the malting quality in 38 wheat varieties. The point value of each parameter was calculated using the regression equations, and the total value of the malting quality of wheat was determined by the modified “superiority measure” method. The results of the assessment of the wheat malting quality showed considerable differences between the varieties. Several varieties exhibited a more pronounced tendency to accumulate nitrogenous substances in grain. Some varieties, even with a slightly higher content of nitrogenous substances, provided above average malt quality. The set included the varieties that exhibited the optimal content of nitrogenous substances in grain, yet they did not give malt of good quality. Spring wheat varieties Alondra, Anabel, and Seance achieved significantly above average malting quality. Dagmar, Evina, Sultan, Rumor, Sailor, and Brokat exhibited slightly above average malting quality. The proposed system for monitoring malting quality of wheat varieties can help find the varieties suitable for the production of quality wheat malt.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"107 1","pages":"232–238-232–238"},"PeriodicalIF":0.0,"publicationDate":"2020-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74916722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carotenoids are produced by photosynthesizing organisms, i.e. plants and algae, as well as some bacteria, protists and fungi. Their role lies mainly in the function of complementary photosynthetic pigments and antioxidants. Carotenoids are distinguished according to their structure into carotenoids of the hydrocarbon type, e.g. α-, β- and γ-carotene and oxygen derivatives – xanthophylls, among which astaxanthin, which excels in its antioxidant and nutraceutical properties, is the most important. It is used as a dietary supplement both in human nutrition and in the aquaculture and poultry industry, as a nutritional supplement in salmonid and hen farming. Many works have focused on the search for natural sources of astaxanthin as an alternative to synthetic astaxanthin. In nature, very few astaxanthin producing organisms are known in non-minor amounts, with green alga Haematococcus pluvialis and the yeast Xanthophyllomyces dendrorhous (anamorph Phaffia rhodozyma) being the most promising microorganisms for the biotechnological production of this xanthophyll. In this review we focus on the biosynthesis and carotenogenesis including the regulation mechanisms, generation of hyperproducing mutants by random mutagenesis and, last but not least, also metabolic engineering (genetic techniques). Attention is also paid to the optimization of fermentation conditions.
{"title":"Yeast need not be used only in beer production. I. The carotenoid astaxanthin produced by yeast.","authors":"T. Řezanka, A. Palyzová","doi":"10.18832/kp2020.66.208","DOIUrl":"https://doi.org/10.18832/kp2020.66.208","url":null,"abstract":"Carotenoids are produced by photosynthesizing organisms, i.e. plants and algae, as well as some bacteria, protists and fungi. Their role lies mainly in the function of complementary photosynthetic pigments and antioxidants. Carotenoids are distinguished according to their structure into carotenoids of the hydrocarbon type, e.g. α-, β- and γ-carotene and oxygen derivatives – xanthophylls, among which astaxanthin, which excels in its antioxidant and nutraceutical properties, is the most important. It is used as a dietary supplement both in human nutrition and in the aquaculture and poultry industry, as a nutritional supplement in salmonid and hen farming. Many works have focused on the search for natural sources of astaxanthin as an alternative to synthetic astaxanthin. In nature, very few astaxanthin producing organisms are known in non-minor amounts, with green alga Haematococcus pluvialis and the yeast Xanthophyllomyces dendrorhous (anamorph Phaffia rhodozyma) being the most promising microorganisms for the biotechnological production of this xanthophyll. In this review we focus on the biosynthesis and carotenogenesis including the regulation mechanisms, generation of hyperproducing mutants by random mutagenesis and, last but not least, also metabolic engineering (genetic techniques). Attention is also paid to the optimization of fermentation conditions.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88828916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Štefániková, V. Nagyová, Matej Hynšt, Dominika Kudláková, J. Árvay, Štefan Dráb
The aim of this work was to compare sensory evaluation and electronic nose technology used to assess aromas in dry hopped beers. An electronic nose based on gas chromatography was used for the first time. Hops varieties Amarillo, Cascade, Chinook, Kazbek and Mandarina Bavaria were used for the production of dry hopped beers and the Sladek variety was used for the control sample. The basic characteristics of the beers were determined, and the sensory evaluation performed by selected assessors was compared to the sensory assay using an electronic nose. Assessment of the aroma profile of dry hopped beers shows that the basic flavours of these beers, such as worty, yeasty and hoppy, were suppressed. Compared to the control sample, a significant grapefruit flavour was noted by the evaluators in Kazbek and Chinook beer samples. The most prominent determinant, compared to the control sample, was in general the citrus aroma. Based on the results of the principal component analysis, it can be concluded that there were statistically significant differences between the individual dry hopped beers and between them and the control sample with the exception of beers dry hopped with hops of the Amarillo and Cascade variety, which was also confirmed by the results of sensory evaluation (approximately the same scoring of the monitored descriptors).
{"title":"A comparison of sensory evaluation and an electronic nose assay in the assessment of aroma in dry hopped beers","authors":"J. Štefániková, V. Nagyová, Matej Hynšt, Dominika Kudláková, J. Árvay, Štefan Dráb","doi":"10.18832/kp2020.66.224","DOIUrl":"https://doi.org/10.18832/kp2020.66.224","url":null,"abstract":"The aim of this work was to compare sensory evaluation and electronic nose technology used to assess aromas in dry hopped beers. An electronic nose based on gas chromatography was used for the first time. Hops varieties Amarillo, Cascade, Chinook, Kazbek and Mandarina Bavaria were used for the production of dry hopped beers and the Sladek variety was used for the control sample. The basic characteristics of the beers were determined, and the sensory evaluation performed by selected assessors was compared to the sensory assay using an electronic nose. Assessment of the aroma profile of dry hopped beers shows that the basic flavours of these beers, such as worty, yeasty and hoppy, were suppressed. Compared to the control sample, a significant grapefruit flavour was noted by the evaluators in Kazbek and Chinook beer samples. The most prominent determinant, compared to the control sample, was in general the citrus aroma. Based on the results of the principal component analysis, it can be concluded that there were statistically significant differences between the individual dry hopped beers and between them and the control sample with the exception of beers dry hopped with hops of the Amarillo and Cascade variety, which was also confirmed by the results of sensory evaluation (approximately the same scoring of the monitored descriptors).","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"1 1","pages":"224–231-224–231"},"PeriodicalIF":0.0,"publicationDate":"2020-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76148900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Karron, E. Runno-Paurson, H. Lõiveke, Bulat Islamov, M. Kütt, T. Talve, E. Lauringson, Hanna Hõrak, L. Edesi, Ü. Niinemets
Fungicides are widely used to reduce Fusarium infections and grain contamination by mycotoxins and increase the yield in cereals, but the efficacy of fungicide treatments in varying climates has not been systematically explored. Field experiments with Estonian spring barley (Hordeum vulgare L.) cv. ‘Maali’ were carried out in three successive years 2012–2014 with strongly varying weather conditions to study the effects of three fungicides, Folicur (active ingredient tebuconazole), Falcon Forte (prothioconazole, tebuconazole, spiroxamine) and Archer Top (fenpropidin, propiconazole), on the yield, incidence of Fusarium spp. and on the contamination of grain with mycotoxins DON, HT-2 and T-2. The fungicides were sprayed once a year at spring barley flowering time. The weather conditions during the three years of study were extremely different. The content of cycotoxin DON, HT2 and T2 was low. The spraying with fungicides had not a clear effect on the barley yield and 1 000 kernel weight, and the study year was primarily the main factor that affected barley yield (p<0.05) and 1 000 kernel weight (p<0.05). The impact of year together with fungicide treatment had a significant effect on the incidence of Fusarium spp. (p<0.05) and on the incidence of mycotoxin DON in barley kernels (p<0.001), but did not have a clear effect on the incidence of mycotoxins HT2 and T2.
{"title":"Application of widely used fungicides does not necessarily affect grain yield, and incidence of Fusarium spp. and mycotoxins DON, HT-2 and T-2 in spring barley in northern climates","authors":"E. Karron, E. Runno-Paurson, H. Lõiveke, Bulat Islamov, M. Kütt, T. Talve, E. Lauringson, Hanna Hõrak, L. Edesi, Ü. Niinemets","doi":"10.18832/kp2020.66.215","DOIUrl":"https://doi.org/10.18832/kp2020.66.215","url":null,"abstract":"Fungicides are widely used to reduce Fusarium infections and grain contamination by mycotoxins and increase the yield in cereals, but the efficacy of fungicide treatments in varying climates has not been systematically explored. Field experiments with Estonian spring barley (Hordeum vulgare L.) cv. ‘Maali’ were carried out in three successive years 2012–2014 with strongly varying weather conditions to study the effects of three fungicides, Folicur (active ingredient tebuconazole), Falcon Forte (prothioconazole, tebuconazole, spiroxamine) and Archer Top (fenpropidin, propiconazole), on the yield, incidence of Fusarium spp. and on the contamination of grain with mycotoxins DON, HT-2 and T-2. The fungicides were sprayed once a year at spring barley flowering time. The weather conditions during the three years of study were extremely different. The content of cycotoxin DON, HT2 and T2 was low. The spraying with fungicides had not a clear effect on the barley yield and 1 000 kernel weight, and the study year was primarily the main factor that affected barley yield (p<0.05) and 1 000 kernel weight (p<0.05). The impact of year together with fungicide treatment had a significant effect on the incidence of Fusarium spp. (p<0.05) and on the incidence of mycotoxin DON in barley kernels (p<0.001), but did not have a clear effect on the incidence of mycotoxins HT2 and T2.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"58 1","pages":"215–223-215–223"},"PeriodicalIF":0.0,"publicationDate":"2020-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84162734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phthalates as esters of phthalic acid and aliphatic alcohol are environmental contaminants which are commonly observed due to their widespread use as plasticizers. Their chemical and physical properties allow them to migrate into the environment and also into foods and beverages easily. The objective of this paper is a summarized overview of phthalate properties, their impact on human health, their content in beverages, especially in beer, including the ways of contamination, and last but not least also legislative regulations.
{"title":"Phthalates in beverages - A review","authors":"Tomáš Horák, J. Olšovská","doi":"10.18832/kp2019.66.264","DOIUrl":"https://doi.org/10.18832/kp2019.66.264","url":null,"abstract":"Phthalates as esters of phthalic acid and aliphatic alcohol are environmental contaminants which are commonly observed due to their widespread use as plasticizers. Their chemical and physical properties allow them to migrate into the environment and also into foods and beverages easily. The objective of this paper is a summarized overview of phthalate properties, their impact on human health, their content in beverages, especially in beer, including the ways of contamination, and last but not least also legislative regulations.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73149114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Scigelová, M. Dušek, Vladimíra Jandovská, J. Olšovská
We analyzed the profile of malt proteins in boiled wort preparations using liquid chromatography coupled to high resolution mass spectrometry. Proteins detected in 9 different malt barley cultivars were identified. We probed proteins for post-translational modifications, namely glycation. Differential analysis of the wort proteomes provided insight into the effect of malting barley cultivar as well as location of cultivation. The results suggest that proteome analysis of wort samples could allow for identification/classification of malting barley cultivars used in wort production.
{"title":"Proteomic Analysis of Wort with Focus on Potential Differentiation of Malting Barley Cultivars","authors":"M. Scigelová, M. Dušek, Vladimíra Jandovská, J. Olšovská","doi":"10.18832/kp2019.65.186","DOIUrl":"https://doi.org/10.18832/kp2019.65.186","url":null,"abstract":"We analyzed the profile of malt proteins in boiled wort preparations using liquid chromatography coupled to high resolution mass spectrometry. Proteins detected in 9 different malt barley cultivars were identified. We probed proteins for post-translational modifications, namely glycation. Differential analysis of the wort proteomes provided insight into the effect of malting barley cultivar as well as location of cultivation. The results suggest that proteome analysis of wort samples could allow for identification/classification of malting barley cultivars used in wort production.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"29 1","pages":"186–191-186–191"},"PeriodicalIF":0.0,"publicationDate":"2019-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73886552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Malting of barley grains is a procedure known for centuries, however, the technology of malt production is constantly being studied and optimized. The optimizations comprise different conditions of barley steeping, as well as using different additives to improve seed germination. Among the most famous group of seed germination regulators, gibberellins, a broad spectrum of diverse chemical substances also have stimulatory effects on seed germination. This review summarizes what is known about multiple barley seed germination promoting compounds, as well as other chemical/hormonal seed germination regulators described in other plant species. The goal is to provide an impulse for testing new stimulatory substances with potential applications in malting process optimization.
{"title":"Seed germination promoting chemical compounds and their potential use in the malting industry","authors":"Hana Dufková","doi":"10.18832/kp2020.66.201","DOIUrl":"https://doi.org/10.18832/kp2020.66.201","url":null,"abstract":"Malting of barley grains is a procedure known for centuries, however, the technology of malt production is constantly being studied and optimized. The optimizations comprise different conditions of barley steeping, as well as using different additives to improve seed germination. Among the most famous group of seed germination regulators, gibberellins, a broad spectrum of diverse chemical substances also have stimulatory effects on seed germination. This review summarizes what is known about multiple barley seed germination promoting compounds, as well as other chemical/hormonal seed germination regulators described in other plant species. The goal is to provide an impulse for testing new stimulatory substances with potential applications in malting process optimization.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"52 1","pages":"201–207-201–207"},"PeriodicalIF":0.0,"publicationDate":"2019-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91160877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beer and brewing raw materials are a source of number of polyphenol substances with potential or proven beneficial biological effects. In pilot brews of Czech pale lager (200 l) the behavior of hop-derived polyphenol substances during wort boiling process in various hopping regimes was studied. Prenylflavonoids, flavonoids and their O-glycosides were determined by liquid chromatography coupled to high resolution mass spectrometry (LC-HR / MS) with sample preparation by the QuEChERS method. The concentration of flavanols (catechin, epicatechin) in hopped wort and beer is dominated by hop raw material, when hopping with aromatic hops rich in polyphenols, 2/3 of the amount in beer is affected by hops. In contrast, the concentration of their mono-O-glucosides in beer does not depend on hopping. Flavonols quercetin, kaempferol and multifidol are found in wort and beer mainly in glycosidic form. The source of all these substances in beer is exclusively hops. Conversely, malt is the only source of myricetin and myricetin-O-glucoside in beer. Main part of hop-derived flavonoids is released during wort boiling into wort within 15-30 minutes. Splitting the hop dose or low pressure boiling technology had no significant effect on health-promoting polyphenols. Beer hopped with hops rich in flavonoids contains a relatively high amount of health-promoting polyphenols, while beer hopped with hop extract is very poor in these antioxidants.
啤酒和酿造原料是许多具有潜在或已证实的有益生物效应的多酚物质的来源。在捷克淡啤酒(200 l)中试啤酒中,研究了不同跳跃条件下啤酒花衍生多酚物质在麦汁沸腾过程中的行为。采用液相色谱-高分辨质谱联用技术(LC-HR / MS)测定丙烯基类黄酮、类黄酮及其o -糖苷,样品制备采用QuEChERS法。酒花麦芽汁和啤酒中黄烷醇(儿茶素、表儿茶素)的含量主要受啤酒花原料的影响,当用富含多酚的芳香啤酒花酿造啤酒时,啤酒中2/3的量受啤酒花的影响。相反,啤酒中它们的单- o-糖苷的浓度不依赖于跳跃。黄酮醇、槲皮素、山奈酚和多元醇主要以糖苷形式存在于麦汁和啤酒中。啤酒中所有这些物质都来自啤酒花。相反,麦芽是啤酒中杨梅素和杨梅素- o -葡萄糖苷的唯一来源。啤酒花类黄酮的主要成分是在15-30分钟的麦汁煮沸过程中释放出来的。拆分啤酒花剂量或低压煮沸工艺对促进健康的多酚含量无显著影响。用富含黄酮类化合物的啤酒花酿造的啤酒含有相对较高的促进健康的多酚,而用啤酒花提取物酿造的啤酒中这些抗氧化剂含量非常低。
{"title":"How wort boiling process affects flavonoid polyphenols in beer","authors":"A. Mikyška, M. Dušek","doi":"10.18832/kp2019.65.192","DOIUrl":"https://doi.org/10.18832/kp2019.65.192","url":null,"abstract":"Beer and brewing raw materials are a source of number of polyphenol substances with potential or proven beneficial biological effects. In pilot brews of Czech pale lager (200 l) the behavior of hop-derived polyphenol substances during wort boiling process in various hopping regimes was studied. Prenylflavonoids, flavonoids and their O-glycosides were determined by liquid chromatography coupled to high resolution mass spectrometry (LC-HR / MS) with sample preparation by the QuEChERS method. The concentration of flavanols (catechin, epicatechin) in hopped wort and beer is dominated by hop raw material, when hopping with aromatic hops rich in polyphenols, 2/3 of the amount in beer is affected by hops. In contrast, the concentration of their mono-O-glucosides in beer does not depend on hopping. Flavonols quercetin, kaempferol and multifidol are found in wort and beer mainly in glycosidic form. The source of all these substances in beer is exclusively hops. Conversely, malt is the only source of myricetin and myricetin-O-glucoside in beer. Main part of hop-derived flavonoids is released during wort boiling into wort within 15-30 minutes. Splitting the hop dose or low pressure boiling technology had no significant effect on health-promoting polyphenols. Beer hopped with hops rich in flavonoids contains a relatively high amount of health-promoting polyphenols, while beer hopped with hop extract is very poor in these antioxidants.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"6 1","pages":"192–200-192–200"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76982378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Many studies have confirmed hops (Humulus lupulus L.) as the source of a number of physiologically active polyphenols, important for both brewing and potentially pharmaceutical purposes. The three-year screening of polyphenols and 23 free phenolics profile (HPLC/coulometric detection) showed a significant association of polyphenols and free phenolics with DPPH antioxidant potential and the genetically determined specificity of free phenolics composition in hops. Varieties of American origin have a different profile compared to European varieties. For the first time, the free phenolics hydroxycoumarins were detected in hops. The DPPH strongly correlated with total polyphenols and flavanoids (n = 95, r = 0.92, 0.93 respectively) and correlated negatively with alpha acids (n = 95, r = -0.73). The polyphenols and the DPPH decrease with increasing alpha acids both among varieties and within one variety. These findings can serve as a useful tool for hop selection in brewing or pharmaceutical production.
许多研究已经证实啤酒花(Humulus lupulus L.)是许多生理活性多酚的来源,对酿造和潜在的药物用途都很重要。为期三年的多酚和23种游离酚谱筛选(HPLC/库伦法检测)表明,多酚和游离酚与DPPH抗氧化能力和遗传决定的啤酒花中游离酚成分的特异性显著相关。与欧洲品种相比,美洲品种具有不同的特征。首次在啤酒花中检测到游离酚类羟基香豆素。DPPH与总多酚和黄酮呈显著正相关(n = 95, r = 0.92, 0.93),与α酸呈显著负相关(n = 95, r = -0.73)。多酚和DPPH在品种间和同一品种内均随α -酸的增加而降低。这些发现可以作为酿造或制药生产中啤酒花选择的有用工具。
{"title":"Varietal specificity of polyphenols, free phenolics and antioxidant potential in hops","authors":"A. Mikyška, M. Jurkovă","doi":"10.18832/kp2019.65.178","DOIUrl":"https://doi.org/10.18832/kp2019.65.178","url":null,"abstract":"Many studies have confirmed hops (Humulus lupulus L.) as the source of a number of physiologically active polyphenols, important for both brewing and potentially pharmaceutical purposes. The three-year screening of polyphenols and 23 free phenolics profile (HPLC/coulometric detection) showed a significant association of polyphenols and free phenolics with DPPH antioxidant potential and the genetically determined specificity of free phenolics composition in hops. Varieties of American origin have a different profile compared to European varieties. For the first time, the free phenolics hydroxycoumarins were detected in hops. The DPPH strongly correlated with total polyphenols and flavanoids (n = 95, r = 0.92, 0.93 respectively) and correlated negatively with alpha acids (n = 95, r = -0.73). The polyphenols and the DPPH decrease with increasing alpha acids both among varieties and within one variety. These findings can serve as a useful tool for hop selection in brewing or pharmaceutical production.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"224 1","pages":"178–185-178–185"},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89164510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Furan is considered to have a potential carcinogenic effect on human health. Its presence was found in a number of foods including beer. Thus, it is necessary to have a fast and routine method for its determination. This study describes a procedure meeting these requirements using the head-space technique associated with gas chromatography. A capillary column with the length of 60 m, inner diameter of 0.32 mm and the film thickness of 0.25 μm was used for the determination of furan. A flame-ionization detector (FID) was used for the detection. The characteristics of the procedure are presented.
{"title":"A novel method of furan determination in beer using gas chromatography with a flame ionization detector","authors":"J. Olšovská, Tomáš Horák, K. Štěrba","doi":"10.18832/kp2019.65.174","DOIUrl":"https://doi.org/10.18832/kp2019.65.174","url":null,"abstract":"Furan is considered to have a potential carcinogenic effect on human health. Its presence was found in a number of foods including beer. Thus, it is necessary to have a fast and routine method for its determination. This study describes a procedure meeting these requirements using the head-space technique associated with gas chromatography. A capillary column with the length of 60 m, inner diameter of 0.32 mm and the film thickness of 0.25 μm was used for the determination of furan. A flame-ionization detector (FID) was used for the detection. The characteristics of the procedure are presented.","PeriodicalId":17834,"journal":{"name":"Kvasny prumysl","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81031378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}