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Sensomics - basic principles and practice 感官学-基本原则和实践
Pub Date : 2019-10-15 DOI: 10.18832/kp2019.65.166
Tomáš Vrzal, J. Olšovská
The article presents basic thoughts and principles of sensomics based on available ´omic´ related literature and own practical experiences of the authors. The sensomics, a member of a wider family of ´omics´ technologies applied to food (foodomics), aims to describe sensory properties of foodstuffs at a molecular level. During the last few years author’s research group has been focused on expansion of this field in brewing science. Strength of the sensomics is in high capability of uncovering hidden information regarding sensory properties and effects of different raw materials as well as technological procedures on sensory properties of foodstuffs and beverages. The article aims to introduce principles and steps of sensomics together with the explanation of their importance, rules and pitfalls. Finally, the practical aspects of sensomic study is presented through sensomic comparison of the influence of decoction and infusion mashing on wort composition.
本文根据现有的“组学”相关文献和作者自己的实践经验,提出了感知学的基本思想和原理。sensomics是应用于食品(食品组学)的更广泛的“组学”技术家族的一员,旨在描述食品在分子水平上的感官特性。在过去的几年里,作者的研究小组一直专注于在酿造科学中扩展这一领域。感官学的优势在于能够发现隐藏的感官特性信息和不同原料对食品和饮料感官特性的影响,以及工艺流程。本文旨在介绍语义学的原理和步骤,并解释其重要性、规则和缺陷。最后,通过对煎煮和浸煮对麦汁成分影响的感官比较,提出了感官研究的实践方面。
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引用次数: 9
The effect of low-temperature plasma discharge on mycotoxin content in barley and malt 低温等离子体放电对大麦和麦芽中霉菌毒素含量的影响
Pub Date : 2019-09-25 DOI: 10.18832/kp2019.65.158
Z. Havelka, S. Běláková, A. Bohatá, I. Hartman, Hana Kábelová, P. Kríž, K. Benešová, M. Dienstbier, P. Bartoš, P. Špatenka
This work deals with the possibility of elimination of common occuring mycotoxins deoxynivalenol (DON) and its plant metabolite deoxynivalenol-3-glucoside (D3G) from malting barley and malt by treatment of low-temperature plasma discharge. Barley seed was treated with a fungicide, entomopathogenic and mycoparasitic fungi and the low-temperature plasma discharge of Gliding Arc type, or their combination. Samples of harvested barley were treated with plasma discharge and analyzed for presence of mycotoxins. Only low DON and D3G concentration levels were detected (maximal values were 48.2 µg/kg and 30.2 µg/kg for DON and D3G, resp.), after plasma treatment mycotoxin content decreased onto the levels near limit of quantification. The results suggest that low-temperature Gliding Arc plasma discharge may contribute to reduction of the content of the mycotoxins in harvested barley grains.
本研究探讨了低温等离子体放电处理大麦和麦芽中常见真菌毒素脱氧雪腐镰梨醇(DON)及其植物代谢物脱氧雪腐镰梨醇-3-葡萄糖苷(D3G)的可能性。采用杀菌剂、昆虫病原真菌和支寄生虫真菌以及滑翔弧型低温等离子体放电或两者组合处理大麦种子。收获的大麦样品用血浆放电处理,并分析是否存在霉菌毒素。仅检测到较低的DON和D3G浓度水平(DON和D3G的最大值分别为48.2µg/kg和30.2µg/kg),血浆处理后霉菌毒素含量下降到接近定量限的水平。结果表明,低温滑行弧等离子体放电可能有助于降低收获大麦籽粒中真菌毒素的含量。
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引用次数: 2
Influence of barley variety and growing locality on the profile of flavonoid polyphenols in malt 大麦品种和产地对麦芽中类黄酮多酚含量的影响
Pub Date : 2019-09-15 DOI: 10.18832/kp2019.65.149
A. Mikyška, M. Dušek, P. Čejka
Some polyphenol substances are important for beer production as well as for the health benefits of beer consumption. Hop polyphenols have been relatively well researched in the last decade, less attention has been paid to malt. Our study was aimed to determine the influence of the barley variety and growing locality on the profile of proanthocyanidins (PAC), monomeric flavanols, flavonols and their glycosides in malt. Laboratory malts from three barley cultivars grown in seven countries of the European Union were analyzed by the developed LC-HR / MS method with sample preparation by the QuEChERS technique. Dimers of (epi)catechin (37.0%) and dimers (epi)catechin – (epi) gallocatechin (36.4%) dominate the PAC profile of malt, monomer content being low. Flavanols (epi)catechin and (epi)catechin-O-glucoside have been identified and quantified in malts. The flavonols were quercetin and myricetin, their glycosides were not detected. The results suggest varietal dependence of the PAC, monomeric flavanols and flavonols profile and the lesser impact of the growing locality.
一些多酚物质对啤酒生产和啤酒消费的健康益处都很重要。近十年来,啤酒花多酚类物质的研究相对较多,而对麦芽的研究较少。本研究旨在确定大麦品种和产地对麦芽中原花青素(PAC)、单体黄烷醇、黄烷醇及其苷类成分的影响。采用采用QuEChERS技术制备样品的LC-HR / MS方法对来自欧盟7个国家的3个大麦品种的实验麦芽进行了分析。(epi)儿茶素二聚体(37.0%)和(epi)儿茶素- (epi)没食子儿茶素二聚体(36.4%)占主导地位,单体含量较低。黄烷醇(epi)儿茶素和(epi)儿茶素- o -葡萄糖苷已在麦芽中鉴定和定量。黄酮醇为槲皮素和杨梅素,未检出其苷类。结果表明,PAC、单体黄烷醇和黄烷醇谱的品种依赖性和生长地点的影响较小。
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引用次数: 4
Microbial xylanases and their inhibition by specific proteins in cereals 谷物中微生物木聚糖酶及其被特定蛋白质抑制的研究
Pub Date : 2019-08-15 DOI: 10.18832/KP2019.65.127
D. Chmelová, Dominika Škulcová, M. Ondrejovič
Arabinoxylans (AXs) belong to the components of plant cells which are mainly degraded by microbial xylanases during colonization of grain by phytopathogens. For the defence, cereals contain proteinaceous xylanase inhibitors (XIs), namely xylanase inhibitor protein (XIP), Triticum aestivum xylanase inhibitor (TAXI) and thaumatin-like xylanase inhibitor (TLXI). Their presence in cereals in high levels can be a serious problem in different industrial applications. XIs regulate AX hydrolysis and participate in plant defence mechanisms. XIs have various specificity against microbial xylanases from the glycoside hydrolase (GH) families of GH10 and GH11. Therefore, this review brings new information about the function of XIs as defence responses to pathogen infection of plants and as a problem in plant material processing in different industrial applications.
阿拉伯木聚糖(Arabinoxylans, AXs)是植物细胞中主要由微生物木聚糖酶在植物病原体定植谷物过程中降解的成分。在防御方面,谷类含有蛋白型木聚糖酶抑制剂(XIs),即木聚糖酶抑制剂蛋白(XIP)、小麦木聚糖酶抑制剂(TAXI)和松素样木聚糖酶抑制剂(TLXI)。它们在谷物中含量过高,在不同的工业应用中可能是一个严重的问题。XIs调节AX水解并参与植物防御机制。XIs对来自GH10和GH11的糖苷水解酶(GH)家族的微生物木聚糖酶具有不同的特异性。因此,本文综述了XIs作为植物对病原菌感染的防御反应的功能以及在不同工业应用中植物材料加工中的问题。
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引用次数: 0
Free amino nitrogen in sweet wort made from barley varieties tested in the Czech Republic 在捷克共和国测试的大麦品种制成的甜麦芽汁中的游离氨基氮
Pub Date : 2019-08-15 DOI: 10.18832/KP2019.65.142
V. Psota
The effect of the variety on the free amino nitrogen (FAN) content in wort was studied in the set of 24 varieties grown at four different testing sites for a three-year period. FAN was determined by the EBC 4.10 method. The highest average FAN was exhibited by the variety KWS Amadora (193 mg/l). The content of FAN was mostly affected by the year (24%), followed by the site, (15%); the effect of the variety was the lowest (13%). Nearly 50% of the samples exhibited low FAN, slightly over 40% of the samples had medium FAN and 12% of the samples exhibited high FAN.
以24个品种为研究对象,在4个不同的试验点生长3年,研究了该品种对麦汁中游离氨基氮(FAN)含量的影响。采用EBC 4.10法测定FAN。品种KWS Amadora的平均FAN最高,为193 mg/l。FAN的含量受年份影响最大(24%),其次是场地(15%);品种的效果最低(13%)。近50%的样本显示低风扇,略高于40%的样本具有中等风扇,12%的样本显示高风扇。
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引用次数: 2
Selenium species in selenium-enriched malt 富硒麦芽中的硒种类
Pub Date : 2019-08-15 DOI: 10.18832/KP2019.65.134
D. Revenco, Marie Vomáčková, L. Jelínek, O. Mestek, R. Koplík
Selenium-enriched malts were prepared by an application of sodium selenite solution to steeped barley grain and following germination and kilning. The changes of selenium valency and the biotransformation of inorganic selenium were investigated by selenium speciation analysis in enzyme-hydrolysed malt samples using liquid chromatography-inductively coupled plasma mass spectrometry. Both inorganic compounds (selenate, selenite) and Se-containing amino acids (selenocystine, Se-methyl-selenocysteine, selenomethionine) were found in green and kilned barley malt.
采用亚硒酸钠溶液浸泡大麦籽粒,经萌发和焙烧制备富硒麦芽。采用液相色谱-电感耦合等离子体质谱法分析酶解麦芽样品中硒的形态,研究硒的价态变化和无机硒的生物转化。在青麦芽和熟麦芽中均发现了无机化合物(硒酸盐、亚硒酸盐)和含硒氨基酸(硒半胱氨酸、硒甲基-硒半胱氨酸、硒代蛋氨酸)。
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引用次数: 5
How does fermentation, filtration and stabilization of beer affect polyphenols with health benefits 啤酒的发酵、过滤和稳定如何影响对健康有益的多酚
Pub Date : 2019-07-01 DOI: 10.18832/KP2019.65.120
A. Mikyška, M. Dušek, Martin Slabý
Phenolic substances affect the quality of beer and some of them have the health benefit for the consumer. Their content in the final product is influenced by a number of raw material-technological factors. We carried out 200 L pilot brews of pale lager with the different maturation period and similar brews focused on the effect of filter material (depth filtration plates /DFP/ and polyester sulfone membrane /PES/) and beer stabilization with protein and polyphenol sorbents. Flavonoids were determined using liquid chromatography in conjunction with high resolution mass spectrometry (LC-HR/ MS) and sample preparation by QuEChERS method. Additionally, total polyphenols and anthocyanogens was measured. The major reduction of both total polyphenols and flavonoids occurred in the first 2–3 weeks of maturation, the longer maturation had no impact on the loss of polyphenols. Filtration of beer with DFP significantly reduced the amount of anthocyanogens, but had no effect on monomeric flavonoid polyphenols. This technique could improve colloidal stability while preserving flavonoids. Conversely, PES membrane filtering greatly reduced the prenylflavonoid content (by 85%), and reduced the amount of flavanols and flavonols at levels comparable to those of PVPP-based polyphenol sorbent (25–35%). Flavonoids in beer could be largely influenced not only by PVPP treatment, but also by membrane filters used for both cold sterilization and primary beer filtration. Protein sorbent stabilization did not affect the content of flavonoids in beer. Decreases in flavonoid glycosides during filtration/stabilization were always lower than those of free flavonoids.
酚类物质影响啤酒的质量,其中一些对消费者有健康益处。它们在最终产品中的含量受到许多原料工艺因素的影响。我们进行了200 L不同成熟期的淡啤酒中试啤酒和类似啤酒,重点研究了过滤材料(深度过滤板/DFP/和聚酯砜膜/PES/)和蛋白质和多酚吸附剂对啤酒稳定性的影响。采用液相色谱-高分辨质谱法(LC-HR/ MS)和QuEChERS制样法测定黄酮类化合物含量。此外,还测定了总多酚和花青素。总多酚和黄酮类化合物的减少主要发生在成熟的前2-3周,较长的成熟时间对多酚的损失没有影响。用DFP过滤啤酒可显著降低啤酒中花青素的含量,但对单体类黄酮多酚没有影响。该技术可以提高胶体稳定性,同时保留黄酮类化合物。相反,PES膜过滤大大降低了戊烯基类黄酮含量(85%),并减少了黄烷醇和黄酮醇的含量,其水平与pvpp基多酚吸附剂相当(25-35%)。PVPP处理对啤酒中黄酮类化合物的含量有很大的影响,冷杀菌和啤酒初过滤的膜过滤器也对啤酒中的黄酮类化合物含量有很大的影响。蛋白质吸附稳定对啤酒中黄酮类化合物的含量没有影响。在过滤/稳定过程中,黄酮类苷的减少量始终低于游离黄酮类的减少量。
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引用次数: 11
Barley varieties registered in the Czech Republic after harvest 2018 2018年收获后在捷克共和国注册的大麦品种
Pub Date : 2019-06-15 DOI: 10.18832/KP2019.65.97
V. Psota, O. Dvořáčková, Milan Nečas, M. Musilová
After harvest 2018 new spring barley malting varieties Cosmopolitan, Ismena, Klarinette, Laureate, LG Aurus, and Runner were registered in the Czech Republic. The spring barley varieties were rich in extract. The varieties Cosmopolitan, Laureate, and LG Aurus had more than 83 % of extract. The values of Kolbach index, diastatic power and apparent final attenuation were at the above average to optimal level in the studied varieties of spring barley. The level of cytolytic modification was at above average to optimal level with the exception of the variety Runner which exhibited higher β-glucan content in wort (192 mg/l). In addition, non-malting varieties of winter six-row varieties Azrah, Impala, Journey, SU Jule, and hybrid variety SU Hylona were registered.
2018年收获后,新的春季大麦麦芽品种Cosmopolitan、Ismena、Klarinette、Laureate、LG Aurus和Runner在捷克共和国注册。春大麦品种提取物丰富。世界性、劳瑞德和LG Aurus的提取物含量超过83%。春大麦品种的科尔巴赫指数、抗旱力和表观最终衰减值均在平均以上至最优水平。除Runner品种的麦汁β-葡聚糖含量较高(192 mg/l)外,其余品种的细胞溶解修饰水平均高于平均水平至最佳水平。此外,还登记了冬季六行品种阿兹拉、黑斑羚、旅程、苏居乐和杂交种苏Hylona等非麦芽品种。
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引用次数: 1
The relation between fatty acids’ chemical profile and chemotaxonomy of Czech barley (Hordeum vulgare) varieties, geographical origin and malting process 捷克大麦(Hordeum vulgare)品种、产地和酿造过程与脂肪酸化学特征的关系
Pub Date : 2019-06-15 DOI: 10.18832/KP2019.65.111
J. Olšovská, Tomáš Vrzal, K. Štěrba, P. Čejka, A. Mikyška
The dependence of FA profiles in barley and malt on variety and geographical origin using an optimized method was determined. FA profiles from 6 Czech varieties, each from 5 localities during two crop years were studied. The extraction of lipids and FA with hexane by fluidized bed extraction and a hydrolysis of lipids prior to derivatization of FA was used. The statistical processing of FA profiles led to a differentiation of samples; the importance of distinguishing parameters decreases in the order of crop year ˃ geographical origin ˃ variety. Nevertheless, the differentiation of tested varieties using FA profiles coresponds to a varietal pedigree. When the total lipid content was considered, a statistical difference between two varieties (Malz and Sunshine) and two tested localities was found. The profile of FA in barley grains is influenced by the land type and climate conditions. Due to a high level of barley varieties crossbreeding, the chemical profiling of FA is not a reliable tool for a varietal determination, however, it reflects the variety origin.
利用优化方法确定了大麦和麦芽中FA谱与品种和产地的关系。研究了来自捷克5个地区的6个品种在2个作物年的FA谱。用己烷流化床萃取法提取脂质和FA,并在FA衍生化前对脂质进行水解。FA谱的统计处理导致了样本的分化;区分参数的重要性按作物年、地理来源、品种顺序依次递减。尽管如此,利用FA谱对被试品种进行分化符合一个品种谱系。当考虑总脂质含量时,发现两个品种(Malz和Sunshine)和两个测试地点之间存在统计学差异。大麦籽粒FA分布受土地类型和气候条件的影响。由于大麦品种杂交水平较高,化学性状分析并不是确定品种的可靠工具,但它反映了品种的起源。
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引用次数: 0
Barley varieties registered in the Slovak Republic after harvest 2018 2018年收获后在斯洛伐克共和国注册的大麦品种
Pub Date : 2019-06-15 DOI: 10.18832/KP2019.65.106
V. Psota, M. Svorad, M. Musilová
After completing the state varietal tests, the spring barley malting varieties Chimbon, Laureate and LG Aurus, and were registered in the Slovak Republic. Extract content in dry matter in the studied varieties was high and moved in the scope of 83.1 – 83.5 %. The varieties had strong proteolytic modification (Kolbach index 49.5 – 50.7 %). Amylolytic and cytolytic modification was at the optimal level. Wort quality was optimal (apparent final attenuation 81.5 – 82.2 %). The studied varieties gave clear wort, with the exception of the variety Accordine with the weakly opalizing wort.
在完成国家品种测试后,春大麦麦芽品种Chimbon, Laureate和LG Aurus在斯洛伐克共和国注册。各品种干物质提取物含量较高,在83.1% ~ 83.5%之间。该品种具有较强的蛋白水解性(科尔巴赫指数为49.5 ~ 50.7%)。酶解淀粉和酶解细胞均处于最佳水平。麦汁质量最佳(表观最终衰减81.5% ~ 82.2%)。除弱乳化麦汁品种外,所研究的品种均为透明麦汁。
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引用次数: 0
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Kvasny prumysl
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