Pub Date : 2018-01-01DOI: 10.5656/KSAE.2018.05.0.016
Lee Changjun, Kim, Dongsoo, Chung YoungHack, Lee Sangmyeong, Lee SuckJun, Lee DongWoon
{"title":"Monitoring of black pine bast scale, Matsucoccus thunbergianae (Homoptera: Margarodidae) using yellow sticky trap.","authors":"Lee Changjun, Kim, Dongsoo, Chung YoungHack, Lee Sangmyeong, Lee SuckJun, Lee DongWoon","doi":"10.5656/KSAE.2018.05.0.016","DOIUrl":"https://doi.org/10.5656/KSAE.2018.05.0.016","url":null,"abstract":"","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"91 1","pages":"143-149"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76393661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5656/KSAE.2018.11.0.054
서보윤, Park chang gu, J. Jung, J. Cho, Gwan‐Seok Lee, 김광호
{"title":"A Loop-mediated Isothermal Amplification Method for White-backed Planthopper-specific Detection","authors":"서보윤, Park chang gu, J. Jung, J. Cho, Gwan‐Seok Lee, 김광호","doi":"10.5656/KSAE.2018.11.0.054","DOIUrl":"https://doi.org/10.5656/KSAE.2018.11.0.054","url":null,"abstract":"","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"1 1","pages":"393-399"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90180496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5656/KSAE.2018.08.0.033
Jinbae Seung, S. H. Lee
{"title":"First Report of Genus Hylis Gozis, 1886 (Coleoptera: Eucnemidae) with a Species, Hylis harmandi (Fleutiaux, 1923) from Korea","authors":"Jinbae Seung, S. H. Lee","doi":"10.5656/KSAE.2018.08.0.033","DOIUrl":"https://doi.org/10.5656/KSAE.2018.08.0.033","url":null,"abstract":"","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"57 1","pages":"271-273"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79894562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5656/KSAE.2017.11.0.044
A. Kim, Young Ha Kim, Bo-Hey Choi, T. Nguyen, K. A. Yoon, S. Lee, H. Han, Y. Koh
Currently, there is no available tool that rapidly diagnoses pine wood nematode (PWN)-infected pine trees in the field. In this study, we synthesized and purified PWN Galectin, which might be an antigen specific to PWN, using the Baculovirus expression system. We used PWN Galectin as an antigen for generating 1,464 fusion hybridoma cell lines secreting monoclonal antibodies (Mabs). Among them, we selected 62 fusion hybridoma cell lines showing high reactivity to PWN Galectin. We further selected 12 fusion hybridoma cell lines showing high reactivity to the standard PWN-infected pine tree phosphate buffered saline (PBS) extract. Additionally, two fusion hybridoma cell lines showing no or extremely low reactivity were used as controls. The selected fusion hybridoma cell lines were subjected to limiting dilutions for selecting and establishing Mab-secreting cell lines showing higher reactivity to the standard PWN-infected pine tree extract than to the standard normal pine tree PBS extract. Moreover, the selected fusion hybridoma cell lines were further selected based on their higher reactivity to PWN protein extracts than to three non-pathogenic nematode protein extracts. The Mab-secreting cell lines established in this study could be used to develop rapid diagnostic tools that can be used in the field or in laboratories for detecting PWN-infected pine trees or PWN.
{"title":"Development of Monoclonal Antibodies Specific to Galectin of Pine Wood Nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle and Their Utilization for Detection of Pine Wood Nematodes","authors":"A. Kim, Young Ha Kim, Bo-Hey Choi, T. Nguyen, K. A. Yoon, S. Lee, H. Han, Y. Koh","doi":"10.5656/KSAE.2017.11.0.044","DOIUrl":"https://doi.org/10.5656/KSAE.2017.11.0.044","url":null,"abstract":"Currently, there is no available tool that rapidly diagnoses pine wood nematode (PWN)-infected pine trees in the field. In this study, we synthesized and purified PWN Galectin, which might be an antigen specific to PWN, using the Baculovirus expression system. We used PWN Galectin as an antigen for generating 1,464 fusion hybridoma cell lines secreting monoclonal antibodies (Mabs). Among them, we selected 62 fusion hybridoma cell lines showing high reactivity to PWN Galectin. We further selected 12 fusion hybridoma cell lines showing high reactivity to the standard PWN-infected pine tree phosphate buffered saline (PBS) extract. Additionally, two fusion hybridoma cell lines showing no or extremely low reactivity were used as controls. The selected fusion hybridoma cell lines were subjected to limiting dilutions for selecting and establishing Mab-secreting cell lines showing higher reactivity to the standard PWN-infected pine tree extract than to the standard normal pine tree PBS extract. Moreover, the selected fusion hybridoma cell lines were further selected based on their higher reactivity to PWN protein extracts than to three non-pathogenic nematode protein extracts. The Mab-secreting cell lines established in this study could be used to develop rapid diagnostic tools that can be used in the field or in laboratories for detecting PWN-infected pine trees or PWN.","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"13 1","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75606844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5656/KSAE.2018.01.1.027
Seulki Kim, J. Koo, Soowon Cho
Conwentzia is a newly recorded genus in Korea, based on C. pineticola. A brief description, photos of adult and male genitalia, and 16S rRNA and COI sequences of the species are provided.
{"title":"Genus Conwentzia (Neuroptera: Coniopterygidae: Coniopteryginae) New to Korea","authors":"Seulki Kim, J. Koo, Soowon Cho","doi":"10.5656/KSAE.2018.01.1.027","DOIUrl":"https://doi.org/10.5656/KSAE.2018.01.1.027","url":null,"abstract":"Conwentzia is a newly recorded genus in Korea, based on C. pineticola. A brief description, photos of adult and male genitalia, and 16S rRNA and COI sequences of the species are provided.","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"15 1","pages":"39-42"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73948462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5656/KSAE.2018.11.0.046
최용석, Hwa-Young Seo, In-Su Whang, 이대홍
We used the entomopathogenic nematode, Steinernema carpocapsae, for the effective biological control of Lycoriella mali during Agaricus bisporus cultivation. The concentrations of two S. carpocapsae products distributed in Korea were 6 × 10 and 2.5 × 10 m respectively. As a result of investigating the density of A. bisporus larvae in a rice straw medium and soil in an A. bisporus cultivation house, the mean numbers per 30 cm were 0.8 and 22.2, respectively, and A. bisporus larvae were detected mainly in the soil. The control effects after once spraying S. carpocapsae at 6 × 10 and 2.5 × 10 m was higher at the 14th day than at the 7th day. Therefore, we investigated the control effect after twice spraying of 6 × 10 and 2.5 × 10 m at the 14th day. The control effects after this spraying were 51.9% and 96.8%, respectively, and the control effect of spraying at 2.5 × 10 m was better than at 6 × 10 m. The control effect of diflubenzuron WP was lower than 50%.
{"title":"Biological Control of the Sciarid Fly, Lycoriella mali (Diptera: Sciaridae) Using Steinernema carpocapsae in a Button Mushroom Cultivation House","authors":"최용석, Hwa-Young Seo, In-Su Whang, 이대홍","doi":"10.5656/KSAE.2018.11.0.046","DOIUrl":"https://doi.org/10.5656/KSAE.2018.11.0.046","url":null,"abstract":"We used the entomopathogenic nematode, Steinernema carpocapsae, for the effective biological control of Lycoriella mali during Agaricus bisporus cultivation. The concentrations of two S. carpocapsae products distributed in Korea were 6 × 10 and 2.5 × 10 m respectively. As a result of investigating the density of A. bisporus larvae in a rice straw medium and soil in an A. bisporus cultivation house, the mean numbers per 30 cm were 0.8 and 22.2, respectively, and A. bisporus larvae were detected mainly in the soil. The control effects after once spraying S. carpocapsae at 6 × 10 and 2.5 × 10 m was higher at the 14th day than at the 7th day. Therefore, we investigated the control effect after twice spraying of 6 × 10 and 2.5 × 10 m at the 14th day. The control effects after this spraying were 51.9% and 96.8%, respectively, and the control effect of spraying at 2.5 × 10 m was better than at 6 × 10 m. The control effect of diflubenzuron WP was lower than 50%.","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"9 1","pages":"355-359"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80166021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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