Reza Melvina Aulia, M. A. Heryanto, Erna Rachmawati, Eddy Renaldi
Tea is one of the leading commodities in Indonesia and is mostly produced in West Java, where Afdeling Sinumbra is one of the plantations that produce Orthodox black tea under the auspices of PT Perkebunan Nusantara VIII but has the smallest production contribution. This indicates that there are problems regarding production, and a risk management analysis is needed. The research was carried out from the plantation and processing levels using the House of Risk method consisting of stages 1 and 2. Stage 1 aims to identify risk events and the causes or sources of these risks. While stage 2 aims to formulate appropriate handling strategies to minimize the impact caused by risk sources. This study shows that crop failure is the biggest risk event at the plantation level stemming from delays in handling pests and diseases. The handling strategy that can be done is to increase discipline. Meanwhile, at the processing level, there is a risk that production and grading are not achieved, which stems from the implementation of SOPs that are not appropriate. The handling strategy that can be done is to warm up the engine, repair the engine, and train.
{"title":"Identifikasi dan Pengendalian Risiko Produksi Teh Hitam Orthodoks Pada PT Perkebunan Nusantara VIII","authors":"Reza Melvina Aulia, M. A. Heryanto, Erna Rachmawati, Eddy Renaldi","doi":"10.30997/jah.v8i2.6352","DOIUrl":"https://doi.org/10.30997/jah.v8i2.6352","url":null,"abstract":"Tea is one of the leading commodities in Indonesia and is mostly produced in West Java, where Afdeling Sinumbra is one of the plantations that produce Orthodox black tea under the auspices of PT Perkebunan Nusantara VIII but has the smallest production contribution. This indicates that there are problems regarding production, and a risk management analysis is needed. The research was carried out from the plantation and processing levels using the House of Risk method consisting of stages 1 and 2. Stage 1 aims to identify risk events and the causes or sources of these risks. While stage 2 aims to formulate appropriate handling strategies to minimize the impact caused by risk sources. This study shows that crop failure is the biggest risk event at the plantation level stemming from delays in handling pests and diseases. The handling strategy that can be done is to increase discipline. Meanwhile, at the processing level, there is a risk that production and grading are not achieved, which stems from the implementation of SOPs that are not appropriate. The handling strategy that can be done is to warm up the engine, repair the engine, and train.","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"31 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114045813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kemasan memiliki peran dalam meningkatkan minat konsumen terhadap pembelian produk. Desain kemasan yang sesuai dengan keinginan konsumen dapat diperoleh dari rancangan kemasan berdasarkan emosi dan perasaan konsumen. Hal ini dapat pula meningkatkan daya saing produk di pasaran. Penelitian ini dilakukan untuk mengidentifikasi kebutuhan konsumen dan menentukan konsep desain visual produk handsanitizer dengan pendekatan Kansei engineering. Metode yang digunakan pada penelitian ini yaitu Term Frequency Inverse Document Frequency (TF-IDF) dan Principal Component Analysis (PCA). Hasil pengidentifikasian kebutuhan konsumen terhadap produk handsanitizer diperoleh 25 kata kansei yang mewakili produk dan ekstraksi kata Kansei pada kemasan handsanitizer menggunakan metode PCA menunjukkan dua konsep desain yaitu Eye catching dan Praktis.
{"title":"Pengembangan Konsep Desain Kemasan Produk Handsanitizer dengan Pendekatan Kansei Engineering","authors":"Delfitriani Delfitriani, Diki, Fina Uzwatania","doi":"10.30997/jah.v8i1.4916","DOIUrl":"https://doi.org/10.30997/jah.v8i1.4916","url":null,"abstract":"Kemasan memiliki peran dalam meningkatkan minat konsumen terhadap pembelian produk. Desain kemasan yang sesuai dengan keinginan konsumen dapat diperoleh dari rancangan kemasan berdasarkan emosi dan perasaan konsumen. Hal ini dapat pula meningkatkan daya saing produk di pasaran. Penelitian ini dilakukan untuk mengidentifikasi kebutuhan konsumen dan menentukan konsep desain visual produk handsanitizer dengan pendekatan Kansei engineering. Metode yang digunakan pada penelitian ini yaitu Term Frequency Inverse Document Frequency (TF-IDF) dan Principal Component Analysis (PCA). Hasil pengidentifikasian kebutuhan konsumen terhadap produk handsanitizer diperoleh 25 kata kansei yang mewakili produk dan ekstraksi kata Kansei pada kemasan handsanitizer menggunakan metode PCA menunjukkan dua konsep desain yaitu Eye catching dan Praktis.","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129723001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRAK Kakao di Kabupaten Kolaka hanya dijual dalam bentuk biji kakao kering dan belum ada yang mampu mengolah menjadi produk lain. Adapun tujuan dari kegiatan ini adalah yaitu (1) meningkatkan pengetahuan cara mengolah biji kakao menjadi bubuk coklat pada kelompok tani kakao (2) meningkatkan keterampilan sekaligus menumbuhkan jiwa wirausaha kelompok tani kakao. Metode kegiatan meliputi observasi lokasi, diikuti dengan penyampaian materi/teori dengan metode ceramah dan diskusi tentang cara pengolahan biji kakao menjadi bubuk coklat tahap selanjutnya dilakukan pelatihan dan praktek langsung yang diakhiri dengan evaluasi. Hasil kegiatan menunjukkan bahwa setelah mengikuti kegiatan ini terjadi peningkatan pemahaman dan pengetahun cara pengolahan biji kakao menjadi bubuk coklat sebesar 90 persen dan pemahaman serta minat kewirausahaan kelompok tani kakao meningkat menjadi 60 persen. Kata kunci : Kakao, inovasi ,penyuluhan, bubuk coklat
{"title":"Tingkat Pemahaman Petani Kakao Terhadap Inovasi Bubuk Coklat","authors":"Mironov Sp","doi":"10.30997/JAH.V6I2.2655","DOIUrl":"https://doi.org/10.30997/JAH.V6I2.2655","url":null,"abstract":"ABSTRAK Kakao di Kabupaten Kolaka hanya dijual dalam bentuk biji kakao kering dan belum ada yang mampu mengolah menjadi produk lain. Adapun tujuan dari kegiatan ini adalah yaitu (1) meningkatkan pengetahuan cara mengolah biji kakao menjadi bubuk coklat pada kelompok tani kakao (2) meningkatkan keterampilan sekaligus menumbuhkan jiwa wirausaha kelompok tani kakao. Metode kegiatan meliputi observasi lokasi, diikuti dengan penyampaian materi/teori dengan metode ceramah dan diskusi tentang cara pengolahan biji kakao menjadi bubuk coklat tahap selanjutnya dilakukan pelatihan dan praktek langsung yang diakhiri dengan evaluasi. Hasil kegiatan menunjukkan bahwa setelah mengikuti kegiatan ini terjadi peningkatan pemahaman dan pengetahun cara pengolahan biji kakao menjadi bubuk coklat sebesar 90 persen dan pemahaman serta minat kewirausahaan kelompok tani kakao meningkat menjadi 60 persen. Kata kunci : Kakao, inovasi ,penyuluhan, bubuk coklat ","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114909493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microencapsulation is a technique that can improve the viability of probiotics and protect the cells from adverse environment by entrapping the cell inside a bead matrix. Probiotic strains Lactobacillus acidophilus was microencapsulated by an emulsion method using sodium alginate, palm oil, and Tween 80. The aim of this research were to produce microencapsulated L. acidophilus by emulsion method and evaluate the stability of microencapsulated probiotic during storage in pineapple fruit butter. Microencapsulation could protect probiotic during storage (significantly different (p=0,05)) than without microencapsulation. During two weeks storage, free cells decreased dramatically (2,5 log CFU/g) than encapsulated cells (1,2 log CFU/g). Storaging microencasulated probiotic in refrigerator temperature (4°C) caused microencapsulated probiotic had longer shelf life (22 days) than room temperature (9 days). pH of pineapple butter containing microencapsulated probiotic cells were found as more stable during storage than were the free cells.
{"title":"Aplikasi Mikroenkapsulasi Lactobacillus acidophilus dalam Butter Buah Nanas (Ananas comosus (L). Merr.)","authors":"D. Lestari, Marvina Marvina, Rianita Pramitasari","doi":"10.30997/jah.v6i2.2788","DOIUrl":"https://doi.org/10.30997/jah.v6i2.2788","url":null,"abstract":"Microencapsulation is a technique that can improve the viability of probiotics and protect the cells from adverse environment by entrapping the cell inside a bead matrix. Probiotic strains Lactobacillus acidophilus was microencapsulated by an emulsion method using sodium alginate, palm oil, and Tween 80. The aim of this research were to produce microencapsulated L. acidophilus by emulsion method and evaluate the stability of microencapsulated probiotic during storage in pineapple fruit butter. Microencapsulation could protect probiotic during storage (significantly different (p=0,05)) than without microencapsulation. During two weeks storage, free cells decreased dramatically (2,5 log CFU/g) than encapsulated cells (1,2 log CFU/g). Storaging microencasulated probiotic in refrigerator temperature (4°C) caused microencapsulated probiotic had longer shelf life (22 days) than room temperature (9 days). pH of pineapple butter containing microencapsulated probiotic cells were found as more stable during storage than were the free cells.","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125054155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The potential for developing coconut commodity plantations in Tiworo Islands, West Muna Regency, Southeast Sulawesi has not been utilized. This study aims to (a) identify the form and type and copra business capacity; and (b) find out the potential of a coconut business located in Tiworo District, West Muna Regency, Southeast Sulawesi. Determination of the location of research carried out deliberately (purposive). The method used is a questionnaire and interview. The analysis used is descriptive-analytical and Location Quotient (LQ) analysis. The results showed that the form of coconut business in the District of Tiworo Kepulauan Muna Barat Regency is classified as a micro business with assets of 50 million each with a total income in the copra business having a garden of Rp. 36,733,125. Then the total coconut business income without processing Rp. 6,493,522 and the total copra business income without having a garden is Rp. 43. 226,647. Potential identification based on the Location Quotient (LQ) calculation shows that the average value of the calculation of a coconut business by owning a plantation and a coconut business without processing of 1.1 is included in the base category, meaning that these two coconut businesses are superior coconut businesses. while the coconut business without owning a garden of 0.8 is classified in the Non-base category or has not yet become a leading business so it needs to be developed further so that this business can become a superior business and be able to compete with other businesses that already exist in the district of Tiworo Islands, West Muna Regency Southeast Sulawesi.Keywords: Potential, Coconut Business, analytical descriptive, Location Quotient, West Muna
在西穆纳摄政岛、苏拉威西岛东南部的蒂沃罗群岛开发椰子商品种植园的潜力尚未得到利用。本研究旨在(a)确定形式、类型和经营能力;(b)了解苏拉威西东南部西穆纳县蒂沃罗区椰子业务的潜力。有意(有目的)确定研究地点。使用的方法是问卷调查和访谈。所使用的分析是描述性分析和位置商(LQ)分析。结果表明，Tiworo Kepulauan Muna Barat Regency地区的椰子业务形式被归类为微型企业，每个资产为5000万，椰子业务的总收入为36,733,125卢比。那么，不加工椰子的总营业收入为Rp. 6,493,522，不拥有花园的椰子总营业收入为Rp. 43。226647年。基于位置商(Location Quotient, LQ)计算的潜在识别表明，拥有种植园的椰子企业和未经加工的椰子企业计算的平均值为1.1被纳入基本类别，这意味着这两家椰子企业是优质椰子企业。而没有拥有花园的椰子业务则为0.8分，属于非基地类，或者尚未成为主导业务，因此需要进一步发展，使该业务能够成为一项优势业务，并能够与苏拉威西岛东南部西穆纳县蒂沃罗群岛地区已经存在的其他业务竞争。关键词:潜力，椰子生意，分析描述，区位商，西部穆纳
{"title":"Identification of Coconut Business Potential with Location Quotient (LQ) in West Muna Regency, Southeast Sulawesi","authors":"Dhian Herdhiansyah","doi":"10.30997/jah.v6i2.2653","DOIUrl":"https://doi.org/10.30997/jah.v6i2.2653","url":null,"abstract":"The potential for developing coconut commodity plantations in Tiworo Islands, West Muna Regency, Southeast Sulawesi has not been utilized. This study aims to (a) identify the form and type and copra business capacity; and (b) find out the potential of a coconut business located in Tiworo District, West Muna Regency, Southeast Sulawesi. Determination of the location of research carried out deliberately (purposive). The method used is a questionnaire and interview. The analysis used is descriptive-analytical and Location Quotient (LQ) analysis. The results showed that the form of coconut business in the District of Tiworo Kepulauan Muna Barat Regency is classified as a micro business with assets of 50 million each with a total income in the copra business having a garden of Rp. 36,733,125. Then the total coconut business income without processing Rp. 6,493,522 and the total copra business income without having a garden is Rp. 43. 226,647. Potential identification based on the Location Quotient (LQ) calculation shows that the average value of the calculation of a coconut business by owning a plantation and a coconut business without processing of 1.1 is included in the base category, meaning that these two coconut businesses are superior coconut businesses. while the coconut business without owning a garden of 0.8 is classified in the Non-base category or has not yet become a leading business so it needs to be developed further so that this business can become a superior business and be able to compete with other businesses that already exist in the district of Tiworo Islands, West Muna Regency Southeast Sulawesi.Keywords: Potential, Coconut Business, analytical descriptive, Location Quotient, West Muna","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127004117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Penelitian ini bertujuan untuk mengembangkan produk minuman sereal instan dari sorgum dan tepung tempe serta untuk mengetahui dan mempelajari tentang karakteristik fisikokimia dan sensori produk minuman sereal instan dari sorgum dan tepung tempe. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan satu faktor dan tiga taraf perlakuan. Faktor perlakuannya adalah perbandingan sorgum dan tepung tempe dengan tiga taraf perlakuan , yaitu (25%:10%), (20%:10%), (15%:15%). Analisis yang dilakukan meliputi uji fisik (daya serap air, waktu rehidrasi, dan viskositas), uji kimia (kadar air, abu, protein, lemak, serat kasar, dan karbohidrat), uji sensori (hedonik). Produk terpilih akan dihitung nilai energi. Berdasarkan hasil data penelitian minuman sereal instan terpilih yaitu perlakuan perbandingan sorgum dan tepung tempe (20%:10%) mengandung daya serap air 269,03 %, waktu rehidrasi 269,50 seconds, viskositas 6,09 cp , kadar air 4,60 %, kadar abu 3,28 %, kadar protein14,81 %, kadar lemak 14,80 %, kadar serat kasar 2,09 %, karbohidrat 62,85 % , total kalori 444 Kkal.
{"title":"KARAKTERISTIK FISIKOKIMIA DAN SENSORI MINUMAN SEREAL INSTAN DARI SORGUM (Sorgum bicolor) DAN TEPUNG TEMPE","authors":"Noli Novidahlia, Intan Kusumaningrum, Aisyah Intan Pamela","doi":"10.30997/JAH.V6I2.3248","DOIUrl":"https://doi.org/10.30997/JAH.V6I2.3248","url":null,"abstract":"Penelitian ini bertujuan untuk mengembangkan produk minuman sereal instan dari sorgum dan tepung tempe serta untuk mengetahui dan mempelajari tentang karakteristik fisikokimia dan sensori produk minuman sereal instan dari sorgum dan tepung tempe. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan satu faktor dan tiga taraf perlakuan. Faktor perlakuannya adalah perbandingan sorgum dan tepung tempe dengan tiga taraf perlakuan , yaitu (25%:10%), (20%:10%), (15%:15%). Analisis yang dilakukan meliputi uji fisik (daya serap air, waktu rehidrasi, dan viskositas), uji kimia (kadar air, abu, protein, lemak, serat kasar, dan karbohidrat), uji sensori (hedonik). Produk terpilih akan dihitung nilai energi. Berdasarkan hasil data penelitian minuman sereal instan terpilih yaitu perlakuan perbandingan sorgum dan tepung tempe (20%:10%) mengandung daya serap air 269,03 %, waktu rehidrasi 269,50 seconds, viskositas 6,09 cp , kadar air 4,60 %, kadar abu 3,28 %, kadar protein14,81 %, kadar lemak 14,80 %, kadar serat kasar 2,09 %, karbohidrat 62,85 % , total kalori 444 Kkal.","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129574949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Di Indonesia, umumnya stainless steel (SS) digunakan sebagai bahan produk peralatan masak diperbagai media minuman atau makanan. Namun investigasi pelepasan logam besi, nikel, mangan dan krom sebagai unsur pemadu SS 200 dan SS 430 ke dalam larutan belum atau sedikit dibahas. Investigasi pelepasan logam-logam tersebut menggunakan metode celup, dimana larutan asam sitrat, larutan garam NaCl dan kombinasinya digunakan sebagai larutan simulasi makanan/minuman. Larutan kontrol juga digunakan berupa air keran buatan. Nilai pelepasan logam diukur dengan menggunakan Atomic Absorption Spectroscopy. Peningkatan pelepasan logam-logam terjadi dalam larutan asam sitrat dibandingkan larutan kontrol, dimana peran larutan NaCl atau campuran larutan NaCl dan asam sitrat juga meningkatkan pelepasan logam. Perlakuan pasivasi awal logam SS juga menurunkan pelepasan logam-logam, dimana indikasi lapisan oksida menahan laju pelepasan logam. Semua nilai pelepasan logam memenuhi kriteria panduan keamanan makanan konsorsium eropa (Council of europe), kecuali krom. Ini mengindikasikan ketidasempurnaan lapisan oksida dalam melapisi permukaan logam, dimana lapisan tersebut mengandung banyak unsur krom.
{"title":"Pelepasan Logam Peralatan Masak Stainless Steel dalam Larutan Simulasi Asam dan Garam","authors":"Gadang Priyotomo","doi":"10.30997/JAH.V6I2.2680","DOIUrl":"https://doi.org/10.30997/JAH.V6I2.2680","url":null,"abstract":"Di Indonesia, umumnya stainless steel (SS) digunakan sebagai bahan produk peralatan masak diperbagai media minuman atau makanan. Namun investigasi pelepasan logam besi, nikel, mangan dan krom sebagai unsur pemadu SS 200 dan SS 430 ke dalam larutan belum atau sedikit dibahas. Investigasi pelepasan logam-logam tersebut menggunakan metode celup, dimana larutan asam sitrat, larutan garam NaCl dan kombinasinya digunakan sebagai larutan simulasi makanan/minuman. Larutan kontrol juga digunakan berupa air keran buatan. Nilai pelepasan logam diukur dengan menggunakan Atomic Absorption Spectroscopy. Peningkatan pelepasan logam-logam terjadi dalam larutan asam sitrat dibandingkan larutan kontrol, dimana peran larutan NaCl atau campuran larutan NaCl dan asam sitrat juga meningkatkan pelepasan logam. Perlakuan pasivasi awal logam SS juga menurunkan pelepasan logam-logam, dimana indikasi lapisan oksida menahan laju pelepasan logam. Semua nilai pelepasan logam memenuhi kriteria panduan keamanan makanan konsorsium eropa (Council of europe), kecuali krom. Ini mengindikasikan ketidasempurnaan lapisan oksida dalam melapisi permukaan logam, dimana lapisan tersebut mengandung banyak unsur krom.","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"192 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123427496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Goat milk is widely consumed as a functional food. Milk’s derived bioactive peptides are the specific peptides which are produced by the degradation of milk protein by proteases enzyme and posseses a different positive properties such as antihypertensive, antioxidant, antibacterial, antiiflamatory and many more. The purpose of this research is to explore the antibacterial activity of fractionated bioactive peptides derived from the hydrolysis of goat milk casein and identify the peptide profiles using SDS-PAGE electrophoresis. Casein was isolated from fresh goat milk and then hydrolyzed by crude bromelain enzyme with an activity of 0.420 U/mL for 0, 1, 2, and 3 minutes at a temperature of 50oC. Profile analysis from bromelain hydrolyzates showed some protein bands with molecular weights from about 6.63 kDa to 7.32 kDa on SDS PAGE gel. The fractionation process was carried out on selected bromelain hydrolyzates using a 30 kDa and 10 kDa membrane Cut-Off. The antibacterial test results showed that the hydrolysates from B0 and B1 peptides showed inhibitory activity on Staphylococcus aureus. The fractionation process increased the antibacterial activity of hydrolysates from peptides B0 and B1. Small molecular weight peptides have better inhibitory activity.
{"title":"Peptida Bioaktif Kasein Susu Kambing sebagai Agen Antibakteri terhadap Staphylococcus aureus","authors":"D. Lestari, Eddyson Giordan","doi":"10.30997/jah.v6i1.2025","DOIUrl":"https://doi.org/10.30997/jah.v6i1.2025","url":null,"abstract":"Goat milk is widely consumed as a functional food. Milk’s derived bioactive peptides are the specific peptides which are produced by the degradation of milk protein by proteases enzyme and posseses a different positive properties such as antihypertensive, antioxidant, antibacterial, antiiflamatory and many more. The purpose of this research is to explore the antibacterial activity of fractionated bioactive peptides derived from the hydrolysis of goat milk casein and identify the peptide profiles using SDS-PAGE electrophoresis. Casein was isolated from fresh goat milk and then hydrolyzed by crude bromelain enzyme with an activity of 0.420 U/mL for 0, 1, 2, and 3 minutes at a temperature of 50oC. Profile analysis from bromelain hydrolyzates showed some protein bands with molecular weights from about 6.63 kDa to 7.32 kDa on SDS PAGE gel. The fractionation process was carried out on selected bromelain hydrolyzates using a 30 kDa and 10 kDa membrane Cut-Off. The antibacterial test results showed that the hydrolysates from B0 and B1 peptides showed inhibitory activity on Staphylococcus aureus. The fractionation process increased the antibacterial activity of hydrolysates from peptides B0 and B1. Small molecular weight peptides have better inhibitory activity.","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129578539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aimed to determine the effect of rebon shirmp (mysis relicta) protein hydrolisate fortification to the sago noodles and to observe the characteristics of the sago noodles produced. The research was carried on in February – April 2019 in the Laboratory of Fish Processing Technology, Integrated Laboratories, and the Fisheries and Marine Chemistry Laboratories at the Universitas Riau. The method used was the experimental method, designedas a non-factorial complete randomized design. The treatment conducted was addingof rebon shrimp protein hydrolysate at 4 level concentrations (0%, 5%, 10% dan 15%). The variables assessed were the quality of organoleptic (appearance, texture, odor, flavor) and the proximate composition. The results showed that the proteinhydrolysate of rebon shrimp at concentration of 15% was the best treatment and in accordance to the quality standards of dried noodles (SNI 01-2974-1996), indicated bythe highest organoleptic qualityof the dried noodles produced, including: the appearance that was brown, less attractive, whole, less neat; the texture that was dry and compact; the aroma that was quite fragrant, spesific rebon shrimp; and the taste that was quite tasty and shrimp flavored. The proximate composition ofthe best product was presented by the content of moisture, ash, fat, protein, and carbohydrate at 7.55%,1.02%, 0.17%, 16.76%, and 74.49%, respectively.Keywords: Hydrolysate protein, rebon shrimp, sago noodles
{"title":"Studi fortifikasi hidrolisat protein udang rebon (mysis relicta) pada mie sagu","authors":"Trie Omitha Purba, S. Suparmi, D. Dahlia","doi":"10.30997/jah.v6i1.1819","DOIUrl":"https://doi.org/10.30997/jah.v6i1.1819","url":null,"abstract":"The study aimed to determine the effect of rebon shirmp (mysis relicta) protein hydrolisate fortification to the sago noodles and to observe the characteristics of the sago noodles produced. The research was carried on in February – April 2019 in the Laboratory of Fish Processing Technology, Integrated Laboratories, and the Fisheries and Marine Chemistry Laboratories at the Universitas Riau. The method used was the experimental method, designedas a non-factorial complete randomized design. The treatment conducted was addingof rebon shrimp protein hydrolysate at 4 level concentrations (0%, 5%, 10% dan 15%). The variables assessed were the quality of organoleptic (appearance, texture, odor, flavor) and the proximate composition. The results showed that the proteinhydrolysate of rebon shrimp at concentration of 15% was the best treatment and in accordance to the quality standards of dried noodles (SNI 01-2974-1996), indicated bythe highest organoleptic qualityof the dried noodles produced, including: the appearance that was brown, less attractive, whole, less neat; the texture that was dry and compact; the aroma that was quite fragrant, spesific rebon shrimp; and the taste that was quite tasty and shrimp flavored. The proximate composition ofthe best product was presented by the content of moisture, ash, fat, protein, and carbohydrate at 7.55%,1.02%, 0.17%, 16.76%, and 74.49%, respectively.Keywords: Hydrolysate protein, rebon shrimp, sago noodles","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"231 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129514619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosy Hutami, Muhammad Fakih Kurniawan, Henna Khoerunnisa
Sumedang tofu is one of favorite foods for Indonesian society. But many sellers or producers are not aware to the food safety of sumedang tofu. The aims of this study were to analyze the microbial, formalin, and lead (Pb) contents in ready-to-eat sumedang tofu which were sold in traffic jams area in Cicurug, Ciawi, and Cisarua. The analysis were carried out by Total Plate Count (TPC) testing for microbial analysis, potassium permanganate reaction (KMnO4) testing for formaldehyde analysis, and atomic absorption spectrophotometry (AAS) method for lead content analysis in the samples. The results obtained for the microbial analysis were sumedang tofu that were sold in the traffic jam areas of Cicurug, Ciawi, and Cisarua contained contaminant above the treshold (1.4 x 105 colonies / gram to 2.2 x 105 colonies / gram of microbes). All of the samples of sumedang tofu were positive containing formaldehyde. Otherwise, there were no lead (Pb) content in all samples regarding to AAS analysis. This study concluded that the ready-to-eat sumedang tofu those were sold in traffic jam area in the Cicurug, Ciawi, and Cisarua were not suitable for consumption because it contained exceed microbial contamination and formalin which are harmful for human health.Keywords : formalin, microbes, sumedang tofu, lead, traffic jam
{"title":"Analisis Kandungan Mikroba, Formalin, dan Timbal (Pb) pada Tahu Sumedang yang Dijual Di Daerah Macet Cicurug, Ciawi, dan Cisarua Jawa Barat","authors":"Rosy Hutami, Muhammad Fakih Kurniawan, Henna Khoerunnisa","doi":"10.30997/jah.v6i1.2385","DOIUrl":"https://doi.org/10.30997/jah.v6i1.2385","url":null,"abstract":"Sumedang tofu is one of favorite foods for Indonesian society. But many sellers or producers are not aware to the food safety of sumedang tofu. The aims of this study were to analyze the microbial, formalin, and lead (Pb) contents in ready-to-eat sumedang tofu which were sold in traffic jams area in Cicurug, Ciawi, and Cisarua. The analysis were carried out by Total Plate Count (TPC) testing for microbial analysis, potassium permanganate reaction (KMnO4) testing for formaldehyde analysis, and atomic absorption spectrophotometry (AAS) method for lead content analysis in the samples. The results obtained for the microbial analysis were sumedang tofu that were sold in the traffic jam areas of Cicurug, Ciawi, and Cisarua contained contaminant above the treshold (1.4 x 105 colonies / gram to 2.2 x 105 colonies / gram of microbes). All of the samples of sumedang tofu were positive containing formaldehyde. Otherwise, there were no lead (Pb) content in all samples regarding to AAS analysis. This study concluded that the ready-to-eat sumedang tofu those were sold in traffic jam area in the Cicurug, Ciawi, and Cisarua were not suitable for consumption because it contained exceed microbial contamination and formalin which are harmful for human health.Keywords : formalin, microbes, sumedang tofu, lead, traffic jam","PeriodicalId":178436,"journal":{"name":"JURNAL AGROINDUSTRI HALAL","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128205294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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