Pub Date : 2023-06-01DOI: 10.11002/kjfp.2023.30.3.383
Min-Ji Park, Han-Cheol Lee, Ji-Young Yang, Jung-Beom Kim
The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss DNA, respectively. The sensitivity of O. keta and O. mykiss primers was 1 ng/μL. Quantitative testing showed that the results met the ‘Guidelines on Standard Procedures for Preparing Analysis Method such as Food’ proposed by the Ministry of Food and Drug Safety. The qPCR method developed and validated in this study for identifying O. keta and O. mykiss has advantages such as speed and field applicability. Therefore, this method is expected to help control forgery and alteration of raw materials in the seafood industry.
{"title":"Development and validation of ultra-fast quantitative real-time PCR\u0000 method to differentiate between Oncorhynchus keta and\u0000 Oncorhynchus mykiss","authors":"Min-Ji Park, Han-Cheol Lee, Ji-Young Yang, Jung-Beom Kim","doi":"10.11002/kjfp.2023.30.3.383","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.3.383","url":null,"abstract":"\u0000 \u0000 The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was\u0000 developed and validated to differentiate the morphologically similar ones,\u0000 Oncorhynchus keta and Oncorhynchus mykiss.\u0000 Species-specific primers were designed for the COI genes of\u0000 mtDNA. The species-specific primers designed for O. keta and\u0000 O. mykiss were selectively amplified by O.\u0000 keta and O. mykiss DNA, respectively. The\u0000 sensitivity of O. keta and O. mykiss primers\u0000 was 1 ng/μL. Quantitative testing showed that the\u0000 results met the ‘Guidelines on Standard Procedures for Preparing Analysis\u0000 Method such as Food’ proposed by the Ministry of Food and Drug Safety.\u0000 The qPCR method developed and validated in this study for identifying O.\u0000 keta and O. mykiss has advantages such as speed\u0000 and field applicability. Therefore, this method is expected to help control\u0000 forgery and alteration of raw materials in the seafood industry.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46481476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}