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Development and validation of ultra-fast quantitative real-time PCR method to differentiate between Oncorhynchus keta and Oncorhynchus mykiss 超快速实时荧光定量PCR方法的建立与验证
Q4 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.11002/kjfp.2023.30.3.383
Min-Ji Park, Han-Cheol Lee, Ji-Young Yang, Jung-Beom Kim
The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss DNA, respectively. The sensitivity of O. keta and O. mykiss primers was 1 ng/μL. Quantitative testing showed that the results met the ‘Guidelines on Standard Procedures for Preparing Analysis Method such as Food’ proposed by the Ministry of Food and Drug Safety. The qPCR method developed and validated in this study for identifying O. keta and O. mykiss has advantages such as speed and field applicability. Therefore, this method is expected to help control forgery and alteration of raw materials in the seafood industry.
建立了一种超快速实时定量聚合酶链反应(qPCR)方法,用于区分形态相似的Oncorhynchus keta和Oncorhynchus mykiss。针对mtDNA的COI基因设计了种特异性引物。分别用O. keta和O. mykiss的DNA选择性扩增了O. keta和O. mykiss的种特异性引物。O. keta和O. mykiss引物的敏感性均为1 ng/μL。定量检测结果符合食品药品安全部提出的《食品等分析方法制备标准程序指南》。本研究开发并验证的qPCR方法鉴定O. keta和O. mykiss具有速度快、现场适用性强等优点。因此,该方法有望帮助控制海鲜行业原材料的伪造和变造。
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引用次数: 0
Application of Bayesian network for farmed eel safety inspection in the production stage 贝叶斯网络在养殖鳗鱼生产阶段安全检测中的应用
Q4 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.11002/kjfp.2023.30.3.459
S. Cho
The Bayesian network (BN) model was applied to analyze the characteristic variables that affect compliance with safety inspections of farmed eel during the production stage, using the data from 30,063 cases of eel aquafarm safety inspection in the Integrated Food Safety Information Network (IFSIN) from 2012 to 2021. The dataset for establishing the BN model included 77 non-conforming cases. Relevant HACCP data, geographic information about the aquafarms, and environmental data were collected and mapped to the IFSIN data to derive explanatory variables for nonconformity. Aquafarm HACCP certification, detection history of harmful substances during the last 5 y, history of nonconformity during the last 5 y, and the suitability of the aquatic environment as determined by the levels of total coliform bacteria and total organic carbon were selected as the explanatory variables. The highest achievable eel aquafarm noncompliance rate by manipulating the derived explanatory variables was 24.5%, which was 94 times higher than the overall farmed eel noncompliance rate reported in IFSIN between 2017 and 2021. The established BN model was validated using the IFSIN eel aquafarm inspection results conducted between January and August 2022. The noncompliance rate in the validation set was 0.22% (15 nonconformances out of 6,785 cases). The precision of BN model prediction was 0.1579, which was 71.4 times higher than the non-compliance rate of the validation set.
利用2012 - 2021年食品安全综合信息网络(IFSIN)中30063例鳗鱼养殖场安全检查数据,应用贝叶斯网络(BN)模型分析了影响养殖鳗鱼生产阶段安全检查合规性的特征变量。建立BN模型的数据集包含77个不符合案例。收集了相关的HACCP数据、养殖场的地理信息和环境数据,并将其映射到IFSIN数据中,以得出不符合的解释变量。选择水产养殖场HACCP认证、最近5y有害物质检测史、最近5y不合格史以及总大肠菌群和总有机碳水平决定的水生环境适宜性作为解释变量。通过操纵衍生解释变量可实现的最高鳗鱼养殖场不合规率为24.5%,是IFSIN在2017年至2021年期间报告的整体养殖鳗鱼不合规率的94倍。利用2022年1月至8月期间进行的IFSIN鳗鱼养殖场检查结果验证了所建立的BN模型。验证集中的不合格率为0.22%(6785例中有15例不合格品)。BN模型预测精度为0.1579,是验证集不符合率的71.4倍。
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引用次数: 0
Quantification of triterpenes in Centella asiatica cultivated in a smart farm, and their effect on keratinocyte activation 智能农场培养积雪草中三萜的定量及其对角质细胞活化的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.11002/kjfp.2023.30.3.483
Jin Hong Park, S. Jo, Da Hee Lee, Youngmin Park, H. Chang, T. Kang, K. Lee
This study aimed to compare the bioactive compounds in Centella asiatica (C. asiatica) cultivated in a smart farm and a field and their effects on human keratinocyte cells. C. asiatica was collected in Jeju-do, Korea, and cultured in a smart farm and a field. The main bioactive compounds in the two differentially cultured C. asiatica were identified, and their activation in keratinocytes were assessed. Amplification and sequencing of the internal transcribed spacer (ITS) DNA in the nucleus and psbA-H DNA in the chloroplast were performed for species analysis. A comparison of DNA of plants reported in the NCBI GenBank was performed. The ITS DNA and psbA-H DNA sequences of C. asiatica cultivated in a smart farm and a field were consistent with No. MH768338.1 and No. JQ425422.1, respectively. Analysis of the triterpenes was performed using high performance liquid chromatography (HPLC) and as a result, C. asiatica cultured in a smart farm had more triterpenes than those cultured in a field. The effects of C. asiatica grown in a smart farm on cell proliferation and scratch recovery in HaCaT cells were greater than those grown in a field. These results suggest that C. asiatica cultivated in a smart farm can be effectively utilized as a health functional food.
本研究旨在比较智能农场和田间栽培的积雪草(c.a asiatica)的生物活性成分及其对人角质形成细胞的影响。在韩国济州岛采集并在智能农场和田地中进行养殖。鉴定了两种不同培养的亚洲木参的主要生物活性成分,并对其在角化细胞中的活性进行了评价。对细胞核内转录间隔区(ITS) DNA和叶绿体内psbA-H DNA进行扩增和测序,用于物种分析。对NCBI GenBank中报道的植物DNA进行了比较。在智能农场和田间栽培的亚洲木曲ITS DNA和psbA-H DNA序列与No. 1一致。MH768338.1和编号:分别JQ425422.1。采用高效液相色谱法(HPLC)对三萜进行了分析,结果表明,智能养殖场培养的亚洲曲中三萜含量高于田间培养的亚洲曲。智能农场培养的亚洲金曲对HaCaT细胞增殖和划痕恢复的影响大于田间培养的。这些结果表明,在智能农场培育的亚洲木臭可以有效地作为一种保健功能食品加以利用。
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引用次数: 0
Physicochemical characteristics of domestic garlic by varieties and their quality changes during aging of black garlic 国产品种大蒜理化特性及黑蒜陈化过程中品质变化
Q4 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.11002/kjfp.2023.30.3.446
Seung-hee Yoo, G. Cha, Dong Gyu Kim, M. Kang
Abstract In this study, the quality characteristics of the representative domestic garlic varieties in Korea (Namdo, Daeseo, Hongsan, and Uiseong garlic) were compared and their changes according to the aging process of black garlic were investigated. Each garlic sample was aged for 15 days according to the manufacturing process of black garlic. The total phenolic compound contents were 85.2-109.7 mg GAE/100 g in raw garlic samples, which increased with the aging period, and Hongsan garlic showed the highest content in both raw and aged black garlic. The content of alliin was 848.3-1,087.5 mg/100 g in raw garlic samples, and it decreased significantly on the 5th day (7.3-20.6 mg/100 g) in aged garlic. The contents of GSAC (γ-glutamyl-S-allyl-cysteine) and GS1PC (γ-glutamyl-S-1-propenyl-l-cysteine) in raw garlic samples were 202.1-541.0 mg/100 g and 311.1-474.3 mg/100 g, respectively, but gradually decreased during the aging process. On the other hand, the SAC (S-allyl-cysteine) and S1PC (S-1-propenyl-l-cysteine) contents of raw garlic were very low in all four varieties, but they were highest on the 5th day of aging and tended to decrease thereafter. On the 5th day of aging, the SAC content of Namdo garlic (208.0 mg/100 g) was highest (p<0.05), and S1PC content was significantly higher in Uiseong garlic (66.2 mg/100 g) than the others.
摘要本研究比较了韩国国内代表性大蒜品种(南岛蒜、大田蒜、洪山蒜和义城蒜)的品质特征,并考察了它们在黑蒜老化过程中的变化。每个大蒜样品按照黑蒜的制作工艺陈化15天。生蒜总酚类化合物含量为85.2 ~ 109.7 mg GAE/100 g,随陈化时间的延长而增加,其中红山蒜在生蒜和陈化黑蒜中含量最高。生蒜中大蒜素含量为848.3 ~ 1087.5 mg/100 g,熟蒜第5天大蒜素含量显著下降(7.3 ~ 20.6 mg/100 g)。生蒜样品中γ-谷氨酰基- s -丙烯基-l-半胱氨酸GSAC含量为202.1 ~ 541.0 mg/100 g, γ-谷氨酰基- s -1-丙烯基-l-半胱氨酸GS1PC含量为311.1 ~ 474.3 mg/100 g,随着老化过程逐渐降低。4个品种生蒜的s -烯丙基半胱氨酸(SAC)和s -1-丙烯基-l-半胱氨酸(S1PC)含量均很低,但在第5 d达到最高,之后呈下降趋势。在老化第5 d,南道大蒜SAC含量最高(208.0 mg/100 g) (p<0.05),义城大蒜S1PC含量显著高于其他品种(66.2 mg/100 g)。
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引用次数: 0
Chemical profiles and biological activities of essential oil of Citrus hystrix DC. peels 红曲霉精油的化学成分和生物活性。果皮
Q4 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.11002/kjfp.2023.30.3.395
Do Minh Long, L. Quoc, Trần Thị Hồng Nhung, Vuong Bao Thy, Nguyen Le Quynh Nhu
Essential oil (EO) was extracted from the peel of Citrus hystrix DC. originating from Tinh Bien, An Giang province (Vietnam), using steam distillation. The study aimed to determine some physicochemical properties of Citrus hystrix peel EO (ChpEO), including the acid value (AV), saponification value (SV), ester value (EV), density, specific gravity, and freezing point. The chemical composition was also analyzed by gas chromatography-mass spectrometry (GC-MS). Compounds like β-pinene (30.19%), D-limonene (22.15%), and sabinene (21.37%), with antioxidant and antibacterial properties, had a relatively high content. The EO was also capable of inhibiting the growth of both Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Salmonella typhimurium (ATCC 13311), and Bacillus cereus (ATCC 11778) specifically.
从红橘皮中提取精油。原产于越南安江省的丁边,使用蒸汽蒸馏。本研究旨在测定红橘皮EO(ChpEO)的一些物理化学性质,包括酸值(AV)、皂化值(SV)、酯值(EV)、密度、比重和冰点。化学成分也通过气相色谱-质谱(GC-MS)进行分析。β-蒎烯(30.19%)、D-柠檬烯(22.15%)和月桂烯(21.37%)等具有抗氧化和抗菌性能的化合物含量相对较高。EO还能够特异性抑制革兰氏阳性菌和革兰氏阴性菌的生长,包括铜绿假单胞菌(ATCC 27853)、金黄色葡萄球菌(ATCC 25923)、鼠伤寒沙门氏菌(ATCC13311)和蜡样芽孢杆菌(ATCC 11778)。
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引用次数: 0
In vitro safety and efficacy of probiotics mixture on carbohydrate digestion inhibition 益生菌混合物抑制碳水化合物消化的体外安全性和有效性
Q4 Agricultural and Biological Sciences Pub Date : 2023-06-01 DOI: 10.11002/kjfp.2023.30.3.538
Eunsol Seo, Jang-Bin Woo, M. Seo, Jeongmin Woo
This study aimed to assess the in vitro safety of a probiotics mixture (Lactobacillus acidophilus PBS066, Lactiplantibacillus plantarum PBS067, and Limosilactobacillus reuteri PBS072), along with its inhibitory effect on carbohydrate digestion. All three strains met the antibiotic resistance profile of the European Food Safety Authority (EFSA) guidelines. None of the strains exhibited hemolytic activity or cytotoxicity against Caco-2 cells. Strains PBS067 and PBS072 inhibited α-amylase activity, whereas all three strains suppressed α-glucosidase activity, indicating that the mixture might limit carbohydrate digestion in the gastrointestinal tract. These findings support the safety of this probiotics mixture and its potential to modulate carbohydrate metabolism in the gut.
本研究旨在评估益生菌混合物(嗜酸乳杆菌PBS066、植物乳杆菌PBS067和路氏利莫西乳杆菌PBS072)的体外安全性及其对碳水化合物消化的抑制作用。这三种菌株都符合欧洲食品安全局(EFSA)指南中的抗生素耐药性。这些菌株均未表现出对Caco-2细胞的溶血活性或细胞毒性。菌株PBS067和PBS072抑制α-淀粉酶活性,而三个菌株都抑制α-葡萄糖苷酶活性,表明混合物可能限制碳水化合物在胃肠道中的消化。这些发现支持了这种益生菌混合物的安全性及其调节肠道碳水化合物代谢的潜力。
{"title":"In vitro safety and efficacy of probiotics mixture\u0000 on carbohydrate digestion inhibition","authors":"Eunsol Seo, Jang-Bin Woo, M. Seo, Jeongmin Woo","doi":"10.11002/kjfp.2023.30.3.538","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.3.538","url":null,"abstract":"\u0000 \u0000 This study aimed to assess the in vitro safety of a probiotics\u0000 mixture (Lactobacillus acidophilus PBS066,\u0000 Lactiplantibacillus plantarum PBS067, and\u0000 Limosilactobacillus reuteri PBS072), along with its\u0000 inhibitory effect on carbohydrate digestion. All three strains met the\u0000 antibiotic resistance profile of the European Food Safety Authority (EFSA)\u0000 guidelines. None of the strains exhibited hemolytic activity or cytotoxicity\u0000 against Caco-2 cells. Strains PBS067 and PBS072 inhibited\u0000 α-amylase activity, whereas all three strains\u0000 suppressed α-glucosidase activity, indicating that the\u0000 mixture might limit carbohydrate digestion in the gastrointestinal tract. These\u0000 findings support the safety of this probiotics mixture and its potential to\u0000 modulate carbohydrate metabolism in the gut.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45942701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physicochemical properties of kombucha with fruit peels during fermentation 康普茶发酵过程中果皮理化性质的研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2023.30.2.321
Tae Yeon Lee, Y. Yi
The study investigated the pH, acidity, soluble solids, total sugar, polyphenol, flavonoid, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and color of kombucha with a variety of added fruit peels during the fermentation process. Pear, grape, plum, orange, apple, and golden kiwi peels were added during fermentation. The pH showed a decrease, while an increase in acidity was observed. An increase in soluble solids, which was higher in most experimental groups than the control group, was also observed. A decrease in total sugar was observed over time. However, an increase was observed in reducing sugar. On Day 0, higher total sugar and reducing sugar were detected in the peel addition group compared with the control group. The antioxidant capacity of polyphenol, flavonoid, anthocyanins, and DPPH radicals scavenging increased with fermentation and was higher in all addition groups, except for pear, compared with the control group. Except for grapes and plums containing high levels of anthocyanins, an increase in the L-value was observed over time, and an increase in the a-value of grapes and plums was also observed (p<0.05). The possible utilization of inedible fruit peel in kombucha was shown. Applying inedible fruit peels to kombucha is proposed to increase antioxidant content and modulate color and pH.
研究了添加不同果皮的康普茶在发酵过程中的pH、酸度、可溶性固形物、总糖、多酚、类黄酮、花青素含量、2,2-二苯基-1-苦基肼(DPPH)自由基清除能力和色泽。发酵过程中加入梨、葡萄、李子、桔子、苹果和金黄色猕猴桃皮。pH值降低,同时观察到酸度增加。还观察到可溶性固形物的增加,在大多数实验组中高于对照组。观察到总糖随着时间的推移而减少。然而,观察到还原糖增加。在第0天,与对照组相比,添加果皮组中检测到更高的总糖和还原糖。多酚、类黄酮、花青素和DPPH自由基的抗氧化能力随发酵而增加,除梨外,所有添加组的抗氧化能力均高于对照组。除了含有高水平花青素的葡萄和李子外,随着时间的推移,观察到L值增加,葡萄和李子的a值也增加(p<0.05)。建议将不可食用的果皮应用于康普茶中,以提高抗氧化剂含量,调节颜色和pH值。
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引用次数: 0
Application of bio-preservation to enhance food safety: A review 生物保鲜在提高食品安全中的应用综述
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2023.30.2.179
Nethma Samadhi Ranathunga, K. Wijayasekara, E. Abeyrathne
Consumers and industry experts frequently have negative perceptions of most chemical preservatives. Although most people concede that they cannot resolve global food waste issues without preservatives, they prefer products without chemical preservatives. Numerous emerging technologies is now surpassing conventional methods for mitigating microbial food deterioration in response to consumer demand and fundamental health and safety considerations, including biological antimicrobial systems such as using food-grade microorganisms and their metabolites primarily originating from microorganisms, plants, and animals. Microbial compounds, including bacteriocins, bacteriophages, and anti-fungal agents, plant extracts such as flavonoids and essential oils; and animal-originated compounds, such as lysozyme, chitosan, and lactoferrin, are considered some of the major bio-preservatives. These natural compounds can be used alone or with other preservatives to improve food safety. Hence, the use of microbes or their metabolic byproducts to extend the shelf life of foods while maintaining safety standards is known as bio-preservation. To manufacture and consume foods in a safe condition, this review primarily aims to broaden knowledge amongst industry professionals and consumers regarding bio-preservation techniques, bio-preservatives, their classifications, and distinctive mechanisms to enhance food safety.
消费者和行业专家经常对大多数化学防腐剂持负面看法。尽管大多数人承认,如果没有防腐剂,他们无法解决全球食品浪费问题,但他们更喜欢没有化学防腐剂的产品。许多新兴技术现在正在超越传统方法来缓解微生物食品变质,以满足消费者需求和基本的健康和安全考虑,包括生物抗菌系统,如使用主要来源于微生物、植物和动物的食品级微生物及其代谢产物。微生物化合物,包括细菌素、噬菌体和抗真菌剂,植物提取物,如类黄酮和精油;动物源性化合物,如溶菌酶、壳聚糖和乳铁蛋白,被认为是一些主要的生物防腐剂。这些天然化合物可以单独使用,也可以与其他防腐剂一起使用,以提高食品安全性。因此,利用微生物或其代谢副产物延长食品的保质期,同时保持安全标准,被称为生物保存。为了在安全的条件下生产和消费食品,本综述主要旨在拓宽行业专业人员和消费者对生物保存技术、生物防腐剂、其分类以及提高食品安全的独特机制的了解。
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引用次数: 0
Effects of chlorine dioxide gas fumigation on the storage quality of astringent persimmon (Diospyros kaki T.) Cheongdobansi 二氧化氯气体熏蒸对涩柿子贮藏品质的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2023.30.2.190
Jiyoon Kim, Jung Soo Kim, Minhyun Kim, Ji Hye Kim, In-Young Kim, I. Nam, Jong-Kuk Kim, K. Moon
Because of their short harvest season, large quantities of persimmons must often be processed within a limited time. Therefore, new methods to extend their storage life are required. This study examined the effects of chlorine dioxide (ClO2) gas fumigation for various treatment periods on the storage quality of astringent persimmons Cheongdobansi under low-temperature conditions. The conditions consisted of continuous treatment with ClO2, treatment for 2 weeks with ClO2, and no treatment, all of which are stored at low temperatures. Control samples (storage 0 days) without any treatment were prepared and all experiments were conducted for 10 weeks at two-week intervals. The ClO2 gas treatment maintained the moisture content, color value, hardness, soluble tannin content, and sensory characteristics. However, ClO2 gas treatment did not affect the soluble solids, pH, and total sugar content. In particular, continuous treatment with ClO2 maintained the storage quality after 6-8 weeks of storage, particularly the hardness and weakness (sensory evaluation). The results suggest the potential of continuous treatment with ClO2 as a highly effective method for maintaining the freshness of Cheongdobansi.
由于收获季节短,大量的柿子通常必须在有限的时间内加工。因此,需要新的方法来延长它们的储存寿命。研究了低温条件下不同时期二氧化氯(ClO2)气体熏蒸对清道板思涩味柿子贮藏品质的影响。实验条件包括连续用ClO2处理、用ClO2处理2周和不处理,均低温保存。制备不加任何处理的对照样品(保存0天),每隔两周进行10周的试验。ClO2气体处理保持了水分含量、色值、硬度、可溶性单宁含量和感官特性。然而,ClO2气体处理对可溶性固形物、pH和总糖含量没有影响。特别是在6-8周后,持续用ClO2处理能保持贮藏质量,尤其是硬度和软度(感官评价)。结果表明,ClO2连续处理是一种保持清道板寺新鲜度的有效方法。
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引用次数: 0
Quality characteristics of in vitro luwak coffee produced using enzyme and microbial complexes 利用酶和微生物复合物制备的离体luwak咖啡的品质特性
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2023.30.2.287
Hye-Mi Kang, Shin-Yeong Oh, Hye-Min Kang, Joong-Ho Kwon, Yong-Jin Jeong
In vitro luwak coffee was produced using enzyme-microbial complexes. The coffee quality of non-fermented coffee beans (NFC) and fermented coffee beans (FC) was compared. The total free amino acid content was higher in FC than in NFC. The levels of glutamic acid and γ-amino-n-butyric acid in NFC were higher than those in FC; however, the contents of essential amino acids, such as lysine, leucine, and valine, in FC were higher than in NFC. During fermentation, the sucrose content decreased, whereas the fructose and glucose contents increased (p<0.001). The chromaticity of the coffee extract showed higher lightness (L), redness (a), and yellowness (b) values in FC than those in NFC. The caffeine content was significantly lower in FC (696.94±0.04 μg/mL) compared to that in NFC (1,130.22±1.55 μg/mL) (p<0.001). Conversely, the polyphenol and chlorogenic acid contents were significantly higher ​​in NFC than in FC (p<0.001). Electronic nose analysis indicated considerable differences between the volatile aromatic components in NFC and FC. Sensory scores were significantly higher for FC than those for NFC. Therefore, the fermentation of coffee beans using enzyme-microbial complexes altered the chemical components, which promoted the Maillard reaction during the coffee bean roasting process. These results suggest the possibility of producing in vitro luwak coffee with better flavor and lower caffeine content.
利用酶-微生物复合物在体外制备luwak咖啡。比较了未发酵咖啡豆(NFC)和发酵咖啡豆(FC)的咖啡品质。游离氨基酸总含量FC高于NFC。NFC中谷氨酸和γ-氨基正丁酸含量高于FC;然而,FC中必需氨基酸如赖氨酸、亮氨酸和缬氨酸的含量高于NFC。发酵过程中,蔗糖含量降低,果糖和葡萄糖含量升高(p<0.001)。与NFC相比,FC中咖啡提取物的亮度(L)、红度(a)和黄度(b)值更高。FC中咖啡因含量(696.94±0.04 μg/mL)显著低于NFC(1130.22±1.55 μg/mL) (p<0.001)。相反,NFC的多酚和绿原酸含量显著高于FC (p<0.001)。电子鼻分析表明,NFC和FC的挥发性芳香成分存在较大差异。FC组的感觉评分明显高于NFC组。因此,利用酶-微生物复合物对咖啡豆进行发酵,改变了咖啡豆的化学成分,促进了咖啡豆烘焙过程中的美拉德反应。这些结果表明,有可能生产出风味更好、咖啡因含量更低的体外luwak咖啡。
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引用次数: 0
期刊
Korean Journal of Food Preservation
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