Pub Date : 2023-04-01DOI: 10.11002/kjfp.2023.30.2.235
Sun Lee, S. Jo, Ji An, Seongsoo Jeong, Dong-Shin Kim, S. Kim, Sukman Park, S. Yun, S. Han, H. Kim
Yellowball (Citrus hybrid cv. Yellowball ) is a new citrus hybrid between Haruka (C. tamurana × natsudaidai ) and Kiyomi (C. unshiu × sinensis) and is known to possess strong antioxidant activity. However, detailed information on the antioxidant components of its peel has not yet been reported. This study evaluated the antioxidant activity of the peel and identified the antioxidant components by fractionating a methanolic extract of Yellowball peels using liquid-liquid extraction with n-hexane, ethyl ether (ether), ethyl acetate (EA), butanol, and water. The phenolic contents and antioxidant activities of the n-hexane, ether, and EA fractions were higher than those of the other fractions, and these fractions were further separated by semi-preparative high-performance liquid chromatography (HPLC). Four antioxidant peaks, EA1, EA2, EA3, and He1, were isolated and analyzed using ultra-performance liquid chromatography–quadrupole-time- of-flight mass spectrometry (UPLC-Q-TOF MS). Sinapoyl glucoside and hesperidin were identified in EA2 and EA3, respectively, and a polymethoxylated flavone (PMF) complex (5-hydroxy-3,6,7,8,3’,4’-hexamethoxyflavone, natsudaidain, tetrameth- oxyflavone, and tangeretin) was identified in He1. A compound in EA1 with m/z 223.0246 [M-H] could not be identified and was named unknown2. The antioxidant activity of unknown2 (IC50=69.17 μg/mL) was similar to that of Trolox, which was noted as a major antioxidant in Yellowball peel. Further studies on the antioxidant capacity of Yellowball peel are required; however, these results provide a foundation for using Yellowball peel as an antioxidant.
{"title":"Chemical profile and antioxidant activity of peel of\u0000 Yellowball, a novel citrus variety","authors":"Sun Lee, S. Jo, Ji An, Seongsoo Jeong, Dong-Shin Kim, S. Kim, Sukman Park, S. Yun, S. Han, H. Kim","doi":"10.11002/kjfp.2023.30.2.235","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.2.235","url":null,"abstract":"\u0000 \u0000 Yellowball (Citrus hybrid cv.\u0000 Yellowball ) is a new citrus hybrid between\u0000 Haruka (C. tamurana ×\u0000 natsudaidai ) and Kiyomi (C.\u0000 unshiu × sinensis) and is known to possess\u0000 strong antioxidant activity. However, detailed information on the antioxidant\u0000 components of its peel has not yet been reported. This study evaluated the\u0000 antioxidant activity of the peel and identified the antioxidant components by\u0000 fractionating a methanolic extract of Yellowball peels using\u0000 liquid-liquid extraction with n-hexane, ethyl ether (ether), ethyl acetate (EA),\u0000 butanol, and water. The phenolic contents and antioxidant activities of the\u0000 n-hexane, ether, and EA fractions were higher than those of the other fractions,\u0000 and these fractions were further separated by semi-preparative high-performance\u0000 liquid chromatography (HPLC). Four antioxidant peaks, EA1, EA2, EA3, and He1,\u0000 were isolated and analyzed using ultra-performance liquid\u0000 chromatography–quadrupole-time- of-flight mass spectrometry\u0000 (UPLC-Q-TOF MS). Sinapoyl glucoside and hesperidin were identified in EA2 and\u0000 EA3, respectively, and a polymethoxylated flavone (PMF) complex\u0000 (5-hydroxy-3,6,7,8,3’,4’-hexamethoxyflavone, natsudaidain,\u0000 tetrameth- oxyflavone, and tangeretin) was identified in He1. A compound\u0000 in EA1 with m/z 223.0246 [M-H] could not be identified and was named unknown2.\u0000 The antioxidant activity of unknown2 (IC50=69.17\u0000 μg/mL) was similar to that of Trolox, which was\u0000 noted as a major antioxidant in Yellowball peel. Further\u0000 studies on the antioxidant capacity of Yellowball peel are\u0000 required; however, these results provide a foundation for using\u0000 Yellowball peel as an antioxidant.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43292918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}