Pub Date : 2024-07-09DOI: 10.15406/ijmboa.2024.07.00175
Ponizovskiy Mr
There were compared the roles of the different methods of Cancer treatments, especially concentrating on advantage of new method of cancer treatment over some negative results appearances of modern methods cancer chemotherapy with large dosage cytotoxic drugs. Thus the current discoveries of influences on the interactions between proteins like CTLA-4, PD-1 and CD-28, CD-80 (B-7-1), CD-86 (B-7-2) on processes for regulation of T cells immune activities that induces to make comparisons to the positive conditions for Cancer immunotherapy versus negative condition for cancer immunotherapy. The immune processes in new method of cancer treatment through Prolonged medical Starvation 42 - 45 days via supporting by extracts of plants with giving very small quantity of cytotoxic substances demand the favourable conditions for concomitant Cancer immunotherapy versus negative conditions of Cancer chemotherapy being created with large dosage cytotoxic drugs. Also there were estimated comparisons of the following methods of Cancer therapies: Surgery therapy, local X-ray therapy, local therapy with radioactive ions. Besides there were shown the influences immune defensive mechanisms on regulatory processes of maintenance stability of Internal Energy in able-bodied organism causing Stationary State of an organism in norm as well as suppression of the influences immune defensive mechanisms on regulatory processes violating maintenance stability Internal Energy of an organism that causes Quasistationary pathologic State of cancer disease metabolic processes. Therefore the favourable condition of mechanism newer method of cancer treatment via “Prolonged medical Starvation (during 42-45 days)” via supporting by herbal extracts induces cancer depression, and using very small dosage weak cytotoxic substances cause destruction cancer cells forming normal condition for operation of immune T cells and macrophages for autophagy promoting best treatment of cancer diseases.
会上比较了不同癌症治疗方法的作用，特别是新的癌症治疗方法对使用大剂量细胞毒性药物的现代癌症化疗方法所产生的一些负面结果的优势。因此，目前发现的 CTLA-4、PD-1 和 CD-28、CD-80 (B-7-1)、CD-86 (B-7-2) 等蛋白质之间的相互作用对 T 细胞免疫活动调节过程的影响，促使人们将癌症免疫疗法的积极条件与癌症免疫疗法的消极条件进行比较。通过长期药物饥饿 42-45 天的癌症治疗新方法中的免疫过程，在植物提取物的支持下，给予极少量的细胞毒性物质，这就要求同时进行癌症免疫治疗的有利条件与使用大剂量细胞毒性药物进行癌症化疗的不利条件进行比较。此外，还对以下癌症治疗方法进行了估计比较：手术疗法、局部 X 射线疗法、放射性离子局部疗法。此外，研究还显示了免疫防御机制对维持健全机体内能稳定的调节过程的影响，从而导致机体处于正常的静止状态，以及抑制免疫防御机制对违反维持机体内能稳定的调节过程的影响，从而导致癌症疾病代谢过程的类静止病理状态。因此，通过中草药提取物支持的 "长期药物饥饿（42-45 天）"癌症治疗新方法的机制的有利条件诱导癌症抑制，并使用非常小剂量的弱细胞毒性物质破坏癌细胞，形成免疫 T 细胞和巨噬细胞自噬运行的正常条件，促进癌症疾病的最佳治疗。
{"title":"The comparison mechanisms of cancer immunotherapy in new method cancer treatment with some modern methods of cancer therapies","authors":"Ponizovskiy Mr","doi":"10.15406/ijmboa.2024.07.00175","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00175","url":null,"abstract":"There were compared the roles of the different methods of Cancer treatments, especially concentrating on advantage of new method of cancer treatment over some negative results appearances of modern methods cancer chemotherapy with large dosage cytotoxic drugs. Thus the current discoveries of influences on the interactions between proteins like CTLA-4, PD-1 and CD-28, CD-80 (B-7-1), CD-86 (B-7-2) on processes for regulation of T cells immune activities that induces to make comparisons to the positive conditions for Cancer immunotherapy versus negative condition for cancer immunotherapy. The immune processes in new method of cancer treatment through Prolonged medical Starvation 42 - 45 days via supporting by extracts of plants with giving very small quantity of cytotoxic substances demand the favourable conditions for concomitant Cancer immunotherapy versus negative conditions of Cancer chemotherapy being created with large dosage cytotoxic drugs. Also there were estimated comparisons of the following methods of Cancer therapies: Surgery therapy, local X-ray therapy, local therapy with radioactive ions. Besides there were shown the influences immune defensive mechanisms on regulatory processes of maintenance stability of Internal Energy in able-bodied organism causing Stationary State of an organism in norm as well as suppression of the influences immune defensive mechanisms on regulatory processes violating maintenance stability Internal Energy of an organism that causes Quasistationary pathologic State of cancer disease metabolic processes. Therefore the favourable condition of mechanism newer method of cancer treatment via “Prolonged medical Starvation (during 42-45 days)” via supporting by herbal extracts induces cancer depression, and using very small dosage weak cytotoxic substances cause destruction cancer cells forming normal condition for operation of immune T cells and macrophages for autophagy promoting best treatment of cancer diseases.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141835915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neurodegenerative diseases pose significant challenges to public health worldwide. MicroRNAs (miRNAs) have emerged as critical regulators of gene expression and are implicated in the pathogenesis of various neurodegenerative disorders. This review comprehensively explores the current understanding of how miRNAs influence gene networks involved in Alzheimer disease, Parkinson disease and amyotrophic lateral sclerosis. We discuss the mechanisms by which miRNAs modulate disease associated genes, their potential as diagnostic biomarkers, and therapeutic targets in neurodegeneration.
{"title":"The Role of MicroRNAs in regulating genes related to neurodegenerative diseases","authors":"Joao Vitor Carvalho Piasentim, Samya Nogueira Asseiss","doi":"10.15406/ijmboa.2024.07.00174","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00174","url":null,"abstract":"Neurodegenerative diseases pose significant challenges to public health worldwide. MicroRNAs (miRNAs) have emerged as critical regulators of gene expression and are implicated in the pathogenesis of various neurodegenerative disorders. This review comprehensively explores the current understanding of how miRNAs influence gene networks involved in Alzheimer disease, Parkinson disease and amyotrophic lateral sclerosis. We discuss the mechanisms by which miRNAs modulate disease associated genes, their potential as diagnostic biomarkers, and therapeutic targets in neurodegeneration.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141695170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-27DOI: 10.15406/ijmboa.2024.07.00162
Philippe Barreto de Almeida, Jéssica Regina Sales Felisberto, Cristiane Flora Villarreal, Renato Crespo Pereira, Davyson de Lima Moreira, Ygor Jessé Ramos
{"title":"Why are there so few studies on plant diversity chemical types? - how ecotypes and chemotypes can unlock insights into environmental and community health","authors":"Philippe Barreto de Almeida, Jéssica Regina Sales Felisberto, Cristiane Flora Villarreal, Renato Crespo Pereira, Davyson de Lima Moreira, Ygor Jessé Ramos","doi":"10.15406/ijmboa.2024.07.00162","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00162","url":null,"abstract":"","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140426741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-13DOI: 10.15406/ijmboa.2024.07.00159
Wendy Yaneth Villastrigo López, Christian Javier Cabello Alvarado, M. D. Dávila Medina, A. C. Castañeda Facio, A. S. Galindo
Lavandula officinalis is a Mediterranean plant to which antimicrobial and antioxidant properties are attributed. The objective of the research was to evaluate the chemical composition, antioxidant and antibacterial activity of dried leaves of Lavandula officinalis. For the extraction of bioactive compounds, ultrasound was used, which is an environmentally friendly technique, where a yield percentage of 15.51% was obtained. The chemical composition was evaluated by high-performance liquid chromatography coupled to mass (HPLC-MS), where it was demonstrated that it was possible to extract compounds such as rosmarinic acid, medioresinol, which are the main compounds to which the properties of this plant are attributed. The antioxidant evaluation was carried out by DPPH, ABTS, and FRAP, where inhibition percentages of 60.99 %, 79.86 %, and 0.07 mg Trolox/mL, respectively, were found. The antibacterial activity against Staphylococcus aureus and Escherichia coli was evaluated by the agar diffusion method, finding inhibition halos of 10 mm and 9 mm, respectively. This demonstrates that the bioactive compounds contained in Lavandula officinalis are an alternative to the synthetic compounds currently used in the cosmetic, food, and pharmaceutical industries due to the properties they possess.
{"title":"Evaluation of antibacterial and antioxidant properties of Lavandula officinalis extracts obtained by ultrasound","authors":"Wendy Yaneth Villastrigo López, Christian Javier Cabello Alvarado, M. D. Dávila Medina, A. C. Castañeda Facio, A. S. Galindo","doi":"10.15406/ijmboa.2024.07.00159","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00159","url":null,"abstract":"Lavandula officinalis is a Mediterranean plant to which antimicrobial and antioxidant properties are attributed. The objective of the research was to evaluate the chemical composition, antioxidant and antibacterial activity of dried leaves of Lavandula officinalis. For the extraction of bioactive compounds, ultrasound was used, which is an environmentally friendly technique, where a yield percentage of 15.51% was obtained. The chemical composition was evaluated by high-performance liquid chromatography coupled to mass (HPLC-MS), where it was demonstrated that it was possible to extract compounds such as rosmarinic acid, medioresinol, which are the main compounds to which the properties of this plant are attributed. The antioxidant evaluation was carried out by DPPH, ABTS, and FRAP, where inhibition percentages of 60.99 %, 79.86 %, and 0.07 mg Trolox/mL, respectively, were found. The antibacterial activity against Staphylococcus aureus and Escherichia coli was evaluated by the agar diffusion method, finding inhibition halos of 10 mm and 9 mm, respectively. This demonstrates that the bioactive compounds contained in Lavandula officinalis are an alternative to the synthetic compounds currently used in the cosmetic, food, and pharmaceutical industries due to the properties they possess.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140457181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-10DOI: 10.15406/ijmboa.2024.07.00157
Sagadevan P, Jenifer Christina, Raghunath M, Nagarajan M, Premalatha S, Preeethi R, Janarthanan P
ceA on the biochemical analysis of this study, which is similar to a battery of biochemical tests including methyl red, Voges-Proskauer, indole and citrate, the organisms were determined to be Klebsiella. In the present study, the highest resistance rate was observed for inoflavuxin (50%) and ceftazidimine (80%), which is similar to the highest resistance rate for cefotaxime (85%) and ceftazidime (68%), with the MIC ranging from 32 to 256 mg/l. The Pseudomonas and Klebsiella plasmid isolation in the present study was found to have a molecular weight of 2.6 kb and 2.7 kb, which is similar to most isolates harboring multiple plasmids ranging in size from about 2 kb to over 150 kb included. Seventy-nine isolates contained plasmids larger than 50 kb, and some contained multiple large plasmids. In the present study, the molecular weight of Pseudomonas and Klebsiella was found to be 356 base pairs and 359 base pairs, respectively, which is similar to that of the 85 clinical isolates of Klebsiella pneumonia screened by PCR amplification of the antiseptic resistance genes qacE1 and ceA.
{"title":"Molecular characterisation of pathogens isolated from egg samples","authors":"Sagadevan P, Jenifer Christina, Raghunath M, Nagarajan M, Premalatha S, Preeethi R, Janarthanan P","doi":"10.15406/ijmboa.2024.07.00157","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00157","url":null,"abstract":"ceA on the biochemical analysis of this study, which is similar to a battery of biochemical tests including methyl red, Voges-Proskauer, indole and citrate, the organisms were determined to be Klebsiella. In the present study, the highest resistance rate was observed for inoflavuxin (50%) and ceftazidimine (80%), which is similar to the highest resistance rate for cefotaxime (85%) and ceftazidime (68%), with the MIC ranging from 32 to 256 mg/l. The Pseudomonas and Klebsiella plasmid isolation in the present study was found to have a molecular weight of 2.6 kb and 2.7 kb, which is similar to most isolates harboring multiple plasmids ranging in size from about 2 kb to over 150 kb included. Seventy-nine isolates contained plasmids larger than 50 kb, and some contained multiple large plasmids. In the present study, the molecular weight of Pseudomonas and Klebsiella was found to be 356 base pairs and 359 base pairs, respectively, which is similar to that of the 85 clinical isolates of Klebsiella pneumonia screened by PCR amplification of the antiseptic resistance genes qacE1 and ceA.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15406/ijmboa.2023.06.00149
Elijah Nya, Atim Peter, O. Udofia
Lycopersicum esculentum Mill often referred to as Tomato is among important vegetable fruits widely cultivated the world over. The fruits are made up of soft endocarp which make them susceptible to spoilage by fungi mold. The fungi produce mycotoxins that are injurious and detrimental to human health. This study is aimed at profiling fungi mold associated with Lycopersicum esculentum rot /spoilage and identify the putative fungi responsible for its spoilage in Nigeria, particularly Akwa Ibom State metropolis. Tomato fruits heavily infested with fungi mold were collected and marshed using a sterile electric blender. Serial dilution of sample in normal saline were made and grown on prepared plates of Potato Dextrose Agar (PDA) to obtain pure culture. High quality genomic DNA was isolated from the samples; it was quantified and checked for purity. The DNA samples were subjected to PCR amplification. The amplicons were gel eluted, sequenced and checked for homology by using Basic local alignment search tool - BLAST. Identification was obtained from the top similarility outcomes of the samples. Four fungal isolates identified were Aspergilus spp., Fusarium spp., Mucor spp. and Penicillium citrinium. The economic importance of fungi molds are not in doubt as some of them are important precursor of diseases as they produces mycotoxins which is capable of causing diseases in both humans and animals, while other sare important in the field of medicine, in industrial production of antibiotics especially Penicillin.
{"title":"Profiling of fungi associated with Lycopersicum esculentum mill. spoilage in Nigeria using internal transcribed spacer (its) sequence of rDNA method","authors":"Elijah Nya, Atim Peter, O. Udofia","doi":"10.15406/ijmboa.2023.06.00149","DOIUrl":"https://doi.org/10.15406/ijmboa.2023.06.00149","url":null,"abstract":"Lycopersicum esculentum Mill often referred to as Tomato is among important vegetable fruits widely cultivated the world over. The fruits are made up of soft endocarp which make them susceptible to spoilage by fungi mold. The fungi produce mycotoxins that are injurious and detrimental to human health. This study is aimed at profiling fungi mold associated with Lycopersicum esculentum rot /spoilage and identify the putative fungi responsible for its spoilage in Nigeria, particularly Akwa Ibom State metropolis. Tomato fruits heavily infested with fungi mold were collected and marshed using a sterile electric blender. Serial dilution of sample in normal saline were made and grown on prepared plates of Potato Dextrose Agar (PDA) to obtain pure culture. High quality genomic DNA was isolated from the samples; it was quantified and checked for purity. The DNA samples were subjected to PCR amplification. The amplicons were gel eluted, sequenced and checked for homology by using Basic local alignment search tool - BLAST. Identification was obtained from the top similarility outcomes of the samples. Four fungal isolates identified were Aspergilus spp., Fusarium spp., Mucor spp. and Penicillium citrinium. The economic importance of fungi molds are not in doubt as some of them are important precursor of diseases as they produces mycotoxins which is capable of causing diseases in both humans and animals, while other sare important in the field of medicine, in industrial production of antibiotics especially Penicillin.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123384241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-14DOI: 10.15406/ijmboa.2023.06.00147
Marlin Touma
Long noncoding RNAs (lncRNAs) have emerged as potent regulators of cardiac development and can drive transcriptome programming, affecting all aspects of gene regulation. The functional properties of lncRNAs are notoriously diverse and have been mechanistically challenging. Thus, focused an efforts for lncRNA annotation and functional interrogation in these contexts is highly required. Herein, we discuss current approaches for identifying putative regulatory lncRNAs for the mechanistic investigation of their function in cardiovascular development and congenital heart defects (CHDs).
{"title":"Regulatory long noncoding RNAs in cardiovascular development and congenital heart defects","authors":"Marlin Touma","doi":"10.15406/ijmboa.2023.06.00147","DOIUrl":"https://doi.org/10.15406/ijmboa.2023.06.00147","url":null,"abstract":"Long noncoding RNAs (lncRNAs) have emerged as potent regulators of cardiac development and can drive transcriptome programming, affecting all aspects of gene regulation. The functional properties of lncRNAs are notoriously diverse and have been mechanistically challenging. Thus, focused an efforts for lncRNA annotation and functional interrogation in these contexts is highly required. Herein, we discuss current approaches for identifying putative regulatory lncRNAs for the mechanistic investigation of their function in cardiovascular development and congenital heart defects (CHDs).","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130687537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-18DOI: 10.15406/ijmboa.2023.06.00145
Grace Oluwatosin Ajayi
This study was carried out to identify, organize and characterize the Patas monkey (Erythrocebus patas) genome. Minimally invasive technique was used to obtain the blood samples for analysis. The blood samples were taken with the assistance of a professional veterinary doctor. They were transferred immediately to blood tubes which contain 95% ethanol for preservation and then stored in refrigerator before laboratory analysis. Genetic analyses revealed that the mitogenome was 16,678 base pair (bp) in length, with an overall base composition of 31.8% A, 25.8% T, 29.7% C, and 12.7% G. The A+T content (57.6%) was greater than the G+C content (42.4%). Phylogenetic analysis was based on the COI sequences of 9 individuals from different locations. The newly sequenced individuals are from Benin Republic, South West, Nigeria and North Central, Nigeria, West Africa; while other sequences were downloaded from the National Center for Biotechnology Information (NCBI database). The nucleotide diversity shows both the frequency and differences between haplotypes, hence, it is a more suitable parameter to estimate the genetic diversity in populations than haplotype diversity. The Kimura-2-parameter (K2P) was used to estimate the genetic distances among the aligned sequences. The study indicates significant genetic differentiation among all of the geographical locations which indicates high genetic distances. The findings revealed a relatively low frequency polymorphisms and absence of variants of alleles among the population of patas. This exemplified a stable demographic history with a stable population size. Hence, the study provides a useful database for analyzing the phylogenetic relationship of Erythrocebus patas. However, further genetic study is recommended for improved conservation of primates in Nigeria.
{"title":"Genetic characterization of patas monkey (Erythrocebus patas) in new-bussa, niger state, Nigeria","authors":"Grace Oluwatosin Ajayi","doi":"10.15406/ijmboa.2023.06.00145","DOIUrl":"https://doi.org/10.15406/ijmboa.2023.06.00145","url":null,"abstract":"This study was carried out to identify, organize and characterize the Patas monkey (Erythrocebus patas) genome. Minimally invasive technique was used to obtain the blood samples for analysis. The blood samples were taken with the assistance of a professional veterinary doctor. They were transferred immediately to blood tubes which contain 95% ethanol for preservation and then stored in refrigerator before laboratory analysis. Genetic analyses revealed that the mitogenome was 16,678 base pair (bp) in length, with an overall base composition of 31.8% A, 25.8% T, 29.7% C, and 12.7% G. The A+T content (57.6%) was greater than the G+C content (42.4%). Phylogenetic analysis was based on the COI sequences of 9 individuals from different locations. The newly sequenced individuals are from Benin Republic, South West, Nigeria and North Central, Nigeria, West Africa; while other sequences were downloaded from the National Center for Biotechnology Information (NCBI database). The nucleotide diversity shows both the frequency and differences between haplotypes, hence, it is a more suitable parameter to estimate the genetic diversity in populations than haplotype diversity. The Kimura-2-parameter (K2P) was used to estimate the genetic distances among the aligned sequences. The study indicates significant genetic differentiation among all of the geographical locations which indicates high genetic distances. The findings revealed a relatively low frequency polymorphisms and absence of variants of alleles among the population of patas. This exemplified a stable demographic history with a stable population size. Hence, the study provides a useful database for analyzing the phylogenetic relationship of Erythrocebus patas. However, further genetic study is recommended for improved conservation of primates in Nigeria.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126036103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-31DOI: 10.15406/ijmboa.2020.05.00126
Okon B, Ibom La, Dauda A, Ebegbulem Vn
Three hundred freshly laid eggs collected from 12 weeks old brown Japanese quails on deep litters fed 18% CP and 2700 kcal ME/kg ration and water ad libitum. External egg quality traits measured included egg (weight and width) and yolk (weight, height and width). Data collected were used to estimate descriptive statistics, phenotypic correlations and to predict egg and yolk weights. Results of phenotypic correlation of external egg traits investigated expressed mostly non-significant (p>0.05) both positive and negative low values with the exceptions of two higher, negative significant (p<0.01) correlations observed between egg weight and egg length (r= – 0.721) and egg weight and shell thickness (r= – 0.616). Similarly, results of phenotypic correlations of internal egg traits expressed mostly non-significant (p>0.05) both positive and negative low values with the exceptions of very high negative correlation coefficient (r= – 0.802) between yolk weight and albumen width and moderate negative correlation coefficient (r= – 0.561) between albumen height and albumen width. The only moderate positive significant correlation (r=0.519) obtained in this study was between yolk weight and albumen height. The regression estimates of parameters and coefficient of determination for simple (one trait) and multiple (two traits and for predicting egg and yolk weight sh6wed very low non-significant associations/interrelationship. But with three to five traits fitted into the multiple linear regression equations, highly significant (p<0.001) associations/interrelationships were obtained for egg and yolk weights. Egg and yolk weights can be predicted using external and internal egg traits measurements with two external traits (egg length and egg width) and two internal traits (albumen height and albumen width) best predicted egg and yolk heights respectively.
{"title":"Egg quality traits, phenotypic correlations, egg and yolk weights prediction using external and internal egg quality traits of Japanese quails reared in Calabar, Nigeria","authors":"Okon B, Ibom La, Dauda A, Ebegbulem Vn","doi":"10.15406/ijmboa.2020.05.00126","DOIUrl":"https://doi.org/10.15406/ijmboa.2020.05.00126","url":null,"abstract":"Three hundred freshly laid eggs collected from 12 weeks old brown Japanese quails on deep litters fed 18% CP and 2700 kcal ME/kg ration and water ad libitum. External egg quality traits measured included egg (weight and width) and yolk (weight, height and width). Data collected were used to estimate descriptive statistics, phenotypic correlations and to predict egg and yolk weights. Results of phenotypic correlation of external egg traits investigated expressed mostly non-significant (p>0.05) both positive and negative low values with the exceptions of two higher, negative significant (p<0.01) correlations observed between egg weight and egg length (r= – 0.721) and egg weight and shell thickness (r= – 0.616). Similarly, results of phenotypic correlations of internal egg traits expressed mostly non-significant (p>0.05) both positive and negative low values with the exceptions of very high negative correlation coefficient (r= – 0.802) between yolk weight and albumen width and moderate negative correlation coefficient (r= – 0.561) between albumen height and albumen width. The only moderate positive significant correlation (r=0.519) obtained in this study was between yolk weight and albumen height. The regression estimates of parameters and coefficient of determination for simple (one trait) and multiple (two traits and for predicting egg and yolk weight sh6wed very low non-significant associations/interrelationship. But with three to five traits fitted into the multiple linear regression equations, highly significant (p<0.001) associations/interrelationships were obtained for egg and yolk weights. Egg and yolk weights can be predicted using external and internal egg traits measurements with two external traits (egg length and egg width) and two internal traits (albumen height and albumen width) best predicted egg and yolk heights respectively.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141219897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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