Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.gg1687n3
LCGC spoke to Leon Barron from Imperial College London, UK, about his work developing a fully miniaturized workflow incorporating a three-dimensional-printed passive sampler device (3D-PSD) to monitor contaminants of emerging concern (CECs) in river water.
{"title":"Monitoring Contaminants of Emerging Concern (CECs) in the Environment","authors":"","doi":"10.56530/lcgc.na.gg1687n3","DOIUrl":"https://doi.org/10.56530/lcgc.na.gg1687n3","url":null,"abstract":"LCGC spoke to Leon Barron from Imperial College London, UK, about his work developing a fully miniaturized workflow incorporating a three-dimensional-printed passive sampler device (3D-PSD) to monitor contaminants of emerging concern (CECs) in river water.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.zi2474s4
Joseph J. Pesek, Maria T. Matyska, Tanya Hiltz, Gary Takeoka
An analytical HPLC method for the identification and quantification of ergothioneine (EGT) has been developed using a silica hydride-based column. A simplified sample preparation method was developed. Several types of commercially available mushrooms were analyzed for quantification of ergothioneine using a Cogent Diamond Hydride column (100 x 4.6 mm) at a flow rate of 1 mL/min, with a deionized water:acetonitrile:0.1% formic acid mobile phase using an aqueous normal phase (ANP) gradient. EGT was detected at 254 nm and was identified by matching retention times of the verified standard. The standard ergothioneine content ranged from 0.01 mg to 0.1 mg/mL with excellent linearity correlation (R2 = 0.99999). EGT recoveries were within 96.5–100.3%. The developed HPLC method was simple, accurate, reproducible, sensitive and rugged.
{"title":"Validation of an Aqueous Normal Phase Chromatography Method for the Analysis of Ergothioneine in Commercial Mushrooms","authors":"Joseph J. Pesek, Maria T. Matyska, Tanya Hiltz, Gary Takeoka","doi":"10.56530/lcgc.na.zi2474s4","DOIUrl":"https://doi.org/10.56530/lcgc.na.zi2474s4","url":null,"abstract":"An analytical HPLC method for the identification and quantification of ergothioneine (EGT) has been developed using a silica hydride-based column. A simplified sample preparation method was developed. Several types of commercially available mushrooms were analyzed for quantification of ergothioneine using a Cogent Diamond Hydride column (100 x 4.6 mm) at a flow rate of 1 mL/min, with a deionized water:acetonitrile:0.1% formic acid mobile phase using an aqueous normal phase (ANP) gradient. EGT was detected at 254 nm and was identified by matching retention times of the verified standard. The standard ergothioneine content ranged from 0.01 mg to 0.1 mg/mL with excellent linearity correlation (R2 = 0.99999). EGT recoveries were within 96.5–100.3%. The developed HPLC method was simple, accurate, reproducible, sensitive and rugged.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.ao6286i9
Dwight R. Stoll
As chromatography technologies change, our approaches to using them in ways that value reliability, as well as the ways we should approach troubleshooting and fixing problems, should also change. In this installment, we survey several protective and troubleshooting strategies, and discuss which approaches have changed over time, and which ones have not. Understanding how the use of these approaches has evolved is helpful both for designing reliability into new methods and when considering updates to legacy methods that have been in use for decades.
{"title":"The Evolution of LC Troubleshooting: Protective Devices and Column Repair","authors":"Dwight R. Stoll","doi":"10.56530/lcgc.na.ao6286i9","DOIUrl":"https://doi.org/10.56530/lcgc.na.ao6286i9","url":null,"abstract":"As chromatography technologies change, our approaches to using them in ways that value reliability, as well as the ways we should approach troubleshooting and fixing problems, should also change. In this installment, we survey several protective and troubleshooting strategies, and discuss which approaches have changed over time, and which ones have not. Understanding how the use of these approaches has evolved is helpful both for designing reliability into new methods and when considering updates to legacy methods that have been in use for decades.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135388745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.ij7373a1
Jonathan Edelman
Chromatography is an essential analytical tool used in many industries, including pharmaceuticals, foodstuffs, and chemistry. It is the most employed technique for separating, identifying, and quantifying components of a mixture. While there are different types of chromatography, the success of all chromatography systems largely depends on the accuracy and precision of their instruments. For the length of my career, nearly every year ushers in giant steps in the systemic improvement of commercial systems’ accuracy, precision, and overall efficiencies. These “leaps” have frequently been attributable to the physical changes in our systems, such as materials, miniaturization, and chemistries. Today, we turn our attention to software, an essential component of chromatography instruments. This article discusses software’s dynamic and growing role in chromatography, its benefits, and the belief that it will profoundly change our jobs and industry.
{"title":"How Software Is Defining the New Era of Laboratory Science","authors":"Jonathan Edelman","doi":"10.56530/lcgc.na.ij7373a1","DOIUrl":"https://doi.org/10.56530/lcgc.na.ij7373a1","url":null,"abstract":"Chromatography is an essential analytical tool used in many industries, including pharmaceuticals, foodstuffs, and chemistry. It is the most employed technique for separating, identifying, and quantifying components of a mixture. While there are different types of chromatography, the success of all chromatography systems largely depends on the accuracy and precision of their instruments. For the length of my career, nearly every year ushers in giant steps in the systemic improvement of commercial systems’ accuracy, precision, and overall efficiencies. These “leaps” have frequently been attributable to the physical changes in our systems, such as materials, miniaturization, and chemistries. Today, we turn our attention to software, an essential component of chromatography instruments. This article discusses software’s dynamic and growing role in chromatography, its benefits, and the belief that it will profoundly change our jobs and industry.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.nr4267c9
Carrie McDonough, Wesley Scott
Per- and polyfluoroalkyl substances (PFASs) are highly persistent anthropogenic compounds that are widespread in the environment. There are thousands of PFASs, yet few neat standards exist for unequivocal identification, quantification, or toxicity assays. The bioanalytical study of complex commercial PFAS mixtures is an innovative route to better understand novel PFAS exposure and toxicity. Here, we highlight efforts using high-resolution mass spectrometry (HRMS) and exposure-relevant mixtures to prioritize PFASs based on their potential to accumulate in living organisms.
{"title":"Tackling PFAS Complexity with HRMS and Bioanalytical Techniques","authors":"Carrie McDonough, Wesley Scott","doi":"10.56530/lcgc.na.nr4267c9","DOIUrl":"https://doi.org/10.56530/lcgc.na.nr4267c9","url":null,"abstract":"Per- and polyfluoroalkyl substances (PFASs) are highly persistent anthropogenic compounds that are widespread in the environment. There are thousands of PFASs, yet few neat standards exist for unequivocal identification, quantification, or toxicity assays. The bioanalytical study of complex commercial PFAS mixtures is an innovative route to better understand novel PFAS exposure and toxicity. Here, we highlight efforts using high-resolution mass spectrometry (HRMS) and exposure-relevant mixtures to prioritize PFASs based on their potential to accumulate in living organisms.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134995597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.fu6271f8
Chris Shevlin, Rahmat Ullah
Despite best efforts in 2004 to ban their use, persistent organic pollutants (POPs) remain prevalent across the globe, including in soil. To protect human health, agricultural and environmental soil require careful investigation, but preparing samples for gas chromatography–mass spectrometry (GC–MS) analysis is time-consuming, and mostly done manually. Accelerated solvent extraction (ASE) has been the preferred preparation method for the past few decades, and while we have seen advancements, the method remains manual. Now, new technology offers parallel sample processing, combined extraction and evaporation, and automation—leading to faster analysis, reduced risk of error, and freed-up time for personnel.
{"title":"New Technologies That Accelerate Persistent Organic Pollutant (POP) Sample Preparation","authors":"Chris Shevlin, Rahmat Ullah","doi":"10.56530/lcgc.na.fu6271f8","DOIUrl":"https://doi.org/10.56530/lcgc.na.fu6271f8","url":null,"abstract":"Despite best efforts in 2004 to ban their use, persistent organic pollutants (POPs) remain prevalent across the globe, including in soil. To protect human health, agricultural and environmental soil require careful investigation, but preparing samples for gas chromatography–mass spectrometry (GC–MS) analysis is time-consuming, and mostly done manually. Accelerated solvent extraction (ASE) has been the preferred preparation method for the past few decades, and while we have seen advancements, the method remains manual. Now, new technology offers parallel sample processing, combined extraction and evaporation, and automation—leading to faster analysis, reduced risk of error, and freed-up time for personnel.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134994585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.av7785a5
Brian Ng, Christine M. Fisher, Susan Genualdi, Wendy Young, Elsie Peprah, Ann M. Knolhoff, Lowri deJager
Accurate quantification of per- and polyfluoroalkyl substances (PFAS) in food is necessary to understand potential dietary exposure. While beneficial for most applications, quantification of PFAS using low-resolution triple quadrupole instruments can be complicated by the presence of co-eluted interferences, which can result in false positives or inaccurate PFAS concentrations. This article discusses the use of high-resolution mass spectrometry (HRMS) to distinguish between matrix interferences and PFAS compounds in a variety of food matrices, and incorporation of these interferences into routine triple quadrupole methods for monitoring.
{"title":"Per- and Polyfluoroalkyl Substances (PFAS) or Interference? Using High-Resolution Mass Spectrometry as an Investigative Tool in Food Analysis","authors":"Brian Ng, Christine M. Fisher, Susan Genualdi, Wendy Young, Elsie Peprah, Ann M. Knolhoff, Lowri deJager","doi":"10.56530/lcgc.na.av7785a5","DOIUrl":"https://doi.org/10.56530/lcgc.na.av7785a5","url":null,"abstract":"Accurate quantification of per- and polyfluoroalkyl substances (PFAS) in food is necessary to understand potential dietary exposure. While beneficial for most applications, quantification of PFAS using low-resolution triple quadrupole instruments can be complicated by the presence of co-eluted interferences, which can result in false positives or inaccurate PFAS concentrations. This article discusses the use of high-resolution mass spectrometry (HRMS) to distinguish between matrix interferences and PFAS compounds in a variety of food matrices, and incorporation of these interferences into routine triple quadrupole methods for monitoring.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134995607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.da4881y5
Simon Hird, Warren Potts, Ken Rosnack
Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals with unique properties. They are used in a wide range of different industries because they repel water and oil, and they are heat-resistant. However, PFAS contamination has become a widespread global concern because of their ubiquitous occurrence, persistence in the environment and living organisms, and potential health risks. Governments around the world are continuously introducing and updating regulatory limits for PFAS in drinking water, other types of water, biota, and, most recently, food. Analyzing such a wide range of sample types for PFAS requires high sensitivity and selectivity, typically provided by methods based on advanced liquid chromatography–tandem quadrupole mass spectrometry (LC–MS/MS) instrumentation. However, one critical aspect to sampling and analyzing PFAS accurately is to avoid PFAS contamination during these processes. This paper emphasizes the significance of robust and reliable PFAS analysis to support efforts to mitigate the global PFAS crisis. Collaboration, research, and advanced analytical solutions are essential to safeguard food, water, and the environment, thusly reducing the impact of PFAS on public health and the environment.
{"title":"Analytical Techniques in Exploring the Persistent Global PFAS Problem","authors":"Simon Hird, Warren Potts, Ken Rosnack","doi":"10.56530/lcgc.na.da4881y5","DOIUrl":"https://doi.org/10.56530/lcgc.na.da4881y5","url":null,"abstract":"Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals with unique properties. They are used in a wide range of different industries because they repel water and oil, and they are heat-resistant. However, PFAS contamination has become a widespread global concern because of their ubiquitous occurrence, persistence in the environment and living organisms, and potential health risks. Governments around the world are continuously introducing and updating regulatory limits for PFAS in drinking water, other types of water, biota, and, most recently, food. Analyzing such a wide range of sample types for PFAS requires high sensitivity and selectivity, typically provided by methods based on advanced liquid chromatography–tandem quadrupole mass spectrometry (LC–MS/MS) instrumentation. However, one critical aspect to sampling and analyzing PFAS accurately is to avoid PFAS contamination during these processes. This paper emphasizes the significance of robust and reliable PFAS analysis to support efforts to mitigate the global PFAS crisis. Collaboration, research, and advanced analytical solutions are essential to safeguard food, water, and the environment, thusly reducing the impact of PFAS on public health and the environment.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134994968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.56530/lcgc.na.ym2073i4
Caroline McGregor, Justin Pennington
Per the U.S. National Academies of Science (NAS) 2019 report “A Research Agenda for Transforming Separation Science,” the number of America’s academic positions in separation science has decreased considerably in recent years, with a number of universities choosing to stop hiring faculty in this area. Specifically, since 1987 (the year of the previous NAS report on this field), the number of faculty members readily identifiable as “separations researchers” has dropped by 40% in the top chemistry departments, and by 30% in the top chemical engineering departments. At the same time, national government funding organizations (such as the National Science Foundation [NSF]) have significantly reduced funding for separation science. All this occurs despite the continuing demand for separation scientists and engineers in the largest economic sectors of the United States. LCGC North America spoke to Caroline McGregor and Justin Pennington of Merck Research Laboratories regarding the effects of the aforementioned occurrences on their firm’s hiring practices, and what employers in general are looking for in candidates they are considering for open separation scientist positions.
根据美国国家科学院(NAS) 2019年的报告“转化分离科学的研究议程”，近年来，美国分离科学的学术职位数量大幅减少，一些大学选择停止招聘该领域的教师。具体来说，自1987年(美国国家科学院就这一领域发表上一份报告的年份)以来，顶级化学系的“分离研究人员”数量下降了40%，顶级化学工程系下降了30%。与此同时，国家政府资助机构(如美国国家科学基金会[NSF])已经大大减少了对分离科学的资助。尽管美国最大的经济部门对分离科学家和工程师的需求持续不断，但所有这些都发生了。LCGC North America采访了默克研究实验室的Caroline McGregor和Justin Pennington，讨论了上述事件对公司招聘行为的影响，以及雇主在考虑开放式分离科学家职位时通常会在候选人身上寻找什么。
{"title":"What Are Employers Looking For When Hiring Separation Scientists?","authors":"Caroline McGregor, Justin Pennington","doi":"10.56530/lcgc.na.ym2073i4","DOIUrl":"https://doi.org/10.56530/lcgc.na.ym2073i4","url":null,"abstract":"Per the U.S. National Academies of Science (NAS) 2019 report “A Research Agenda for Transforming Separation Science,” the number of America’s academic positions in separation science has decreased considerably in recent years, with a number of universities choosing to stop hiring faculty in this area. Specifically, since 1987 (the year of the previous NAS report on this field), the number of faculty members readily identifiable as “separations researchers” has dropped by 40% in the top chemistry departments, and by 30% in the top chemical engineering departments. At the same time, national government funding organizations (such as the National Science Foundation [NSF]) have significantly reduced funding for separation science. All this occurs despite the continuing demand for separation scientists and engineers in the largest economic sectors of the United States. LCGC North America spoke to Caroline McGregor and Justin Pennington of Merck Research Laboratories regarding the effects of the aforementioned occurrences on their firm’s hiring practices, and what employers in general are looking for in candidates they are considering for open separation scientist positions.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136300227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.56530/lcgc.na.jq6284c9
Will Wetzel
The fallout from the Covid-19 global pandemic remains in progress in 2023. Because of the current ongoing global recession, we anticipated that many workers would be concerned about their current jobs and how to adjust to rising inflation. In this year’s LCGC Salary Survey, we sought to explore these topics. In addition to compiling information about the average salary trends and reported on how these trends compare to previous years, we focused this edition of our questionnaire on the current economy, asking respondents to offer their opinions on whether a recession is taking place where they reside, and what impact it is having on them mentally and financially. The results from our survey indicate that anxiety about the current state of the economy was on respondents’ minds in 2023.
{"title":"The 2023 LCGC Salary Survey: How Workers Are Navigating A Tough Economic Landscape","authors":"Will Wetzel","doi":"10.56530/lcgc.na.jq6284c9","DOIUrl":"https://doi.org/10.56530/lcgc.na.jq6284c9","url":null,"abstract":"The fallout from the Covid-19 global pandemic remains in progress in 2023. Because of the current ongoing global recession, we anticipated that many workers would be concerned about their current jobs and how to adjust to rising inflation. In this year’s LCGC Salary Survey, we sought to explore these topics. In addition to compiling information about the average salary trends and reported on how these trends compare to previous years, we focused this edition of our questionnaire on the current economy, asking respondents to offer their opinions on whether a recession is taking place where they reside, and what impact it is having on them mentally and financially. The results from our survey indicate that anxiety about the current state of the economy was on respondents’ minds in 2023.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135053244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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