Pub Date : 2015-12-15Epub Date: 2015-11-11DOI: 10.4049/jimmunol.1402713
Sankar Baruah, Kathy Keck, Michelle Vrenios, Marshall R Pope, Merideth Pearl, Kevin Doerschug, Julia Klesney-Tait
Triggering receptor expressed on myeloid cells-1 (TREM-1) is critical for inflammatory signal amplification. Humans have two forms of TREM-1: a membrane receptor, associated with the adaptor DAP12, and a soluble receptor detected at times of infection. The membrane receptor isoform acts synergistically with the TLR pathway to promote cytokine secretion and neutrophil migration, whereas the soluble receptor functions as a counterregulatory molecule. In multiple models of sepsis, exogenous administration of soluble forms of TREM-1 attenuates inflammation and markedly improves survival. Despite intense interest in soluble TREM-1, both as a clinical predictor of survival and as a therapeutic tool, the origin of native soluble TREM-1 remains controversial. Using human neutrophils, we identified a 15-kDa TREM-1 isoform in primary (azurophilic) and secondary (specific) granules. Mass spectrometric analysis, ELISA, and immunoblot confirm that the 15-kDa protein is a novel splice variant form of TREM-1 (TREM-1sv). Neutrophil stimulation with Pseudomonas aeruginosa, LPS, or PAM(3)Cys4 resulted in degranulation and release of TREM-1sv. The addition of exogenous TREM-1sv inhibited TREM-1 receptor-mediated proinflammatory cytokine production. Thus, these data reveal that TREM-1 isoforms simultaneously activate and inhibit inflammation via the canonical membrane TREM-1 molecule and this newly discovered granular isoform, TREM-1sv.
{"title":"Identification of a Novel Splice Variant Isoform of TREM-1 in Human Neutrophil Granules.","authors":"Sankar Baruah, Kathy Keck, Michelle Vrenios, Marshall R Pope, Merideth Pearl, Kevin Doerschug, Julia Klesney-Tait","doi":"10.4049/jimmunol.1402713","DOIUrl":"10.4049/jimmunol.1402713","url":null,"abstract":"<p><p>Triggering receptor expressed on myeloid cells-1 (TREM-1) is critical for inflammatory signal amplification. Humans have two forms of TREM-1: a membrane receptor, associated with the adaptor DAP12, and a soluble receptor detected at times of infection. The membrane receptor isoform acts synergistically with the TLR pathway to promote cytokine secretion and neutrophil migration, whereas the soluble receptor functions as a counterregulatory molecule. In multiple models of sepsis, exogenous administration of soluble forms of TREM-1 attenuates inflammation and markedly improves survival. Despite intense interest in soluble TREM-1, both as a clinical predictor of survival and as a therapeutic tool, the origin of native soluble TREM-1 remains controversial. Using human neutrophils, we identified a 15-kDa TREM-1 isoform in primary (azurophilic) and secondary (specific) granules. Mass spectrometric analysis, ELISA, and immunoblot confirm that the 15-kDa protein is a novel splice variant form of TREM-1 (TREM-1sv). Neutrophil stimulation with Pseudomonas aeruginosa, LPS, or PAM(3)Cys4 resulted in degranulation and release of TREM-1sv. The addition of exogenous TREM-1sv inhibited TREM-1 receptor-mediated proinflammatory cytokine production. Thus, these data reveal that TREM-1 isoforms simultaneously activate and inhibit inflammation via the canonical membrane TREM-1 molecule and this newly discovered granular isoform, TREM-1sv.</p>","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"19 1","pages":"5725-31"},"PeriodicalIF":0.0,"publicationDate":"2015-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88054388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultra High Efficiency Protein Separations with Submicrometer Silica Using Slip Flow.","authors":"Benjamin J Rogers, Bingchuan Wei, Mary J Wirth","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"30 10","pages":"890-897"},"PeriodicalIF":0.9,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176710/pdf/nihms426491.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32706953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of a formal method transfer is to ensure that the receiving laboratory is well trained, qualified to run the method in question, and gets the same results witing experimental error as the iniating laboratory. The development and validation of robust methods and strict adherence to well documented standart operating procedures is the best way to ensure the ultimate sucess of the methode. This installment of ''Validation Viewponit'' examines the analytical method transfer process, including protocol, documentation, and some possible chromatographic pitfalls to avoid.
{"title":"Analytical Method Transfer","authors":"M. Swartz, I. Krull","doi":"10.1201/B12039-9","DOIUrl":"https://doi.org/10.1201/B12039-9","url":null,"abstract":"The objective of a formal method transfer is to ensure that the receiving laboratory is well trained, qualified to run the method in question, and gets the same results witing experimental error as the iniating laboratory. The development and validation of robust methods and strict adherence to well documented standart operating procedures is the best way to ensure the ultimate sucess of the methode. This installment of ''Validation Viewponit'' examines the analytical method transfer process, including protocol, documentation, and some possible chromatographic pitfalls to avoid.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"24 1","pages":"179-188"},"PeriodicalIF":0.9,"publicationDate":"2012-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1201/B12039-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65975173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-11-03DOI: 10.1002/9780470530191.CH3
R. Montes, T. Riveros, Froseen Dahdouh, G. Hanrahan, F. Gomez
A synopsis of the authors' work detailing the use of chemometric response surface methodology (RSM) in two capillary electrophoresis (CE) studies is described. They demonstrate the usefulness of employing RSM to specific CE applications, thereby proving the utility of chemometrics design in experimental optimization.
{"title":"Chemometrical Experimental Design‐Based Optimization Studies in Capillary Electrophoresis Applications","authors":"R. Montes, T. Riveros, Froseen Dahdouh, G. Hanrahan, F. Gomez","doi":"10.1002/9780470530191.CH3","DOIUrl":"https://doi.org/10.1002/9780470530191.CH3","url":null,"abstract":"A synopsis of the authors' work detailing the use of chemometric response surface methodology (RSM) in two capillary electrophoresis (CE) studies is described. They demonstrate the usefulness of employing RSM to specific CE applications, thereby proving the utility of chemometrics design in experimental optimization.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"125 6","pages":"75-91"},"PeriodicalIF":0.9,"publicationDate":"2009-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50658097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-06-01DOI: 10.1142/9781860949449_0005
L. Ettre
On the occasion of the centenary of the invention of chromatography, this month's Milestones in Chromatography column chronicles the life and activities of its inventor, his struggle for acceptance, and, finally, the triumph of his technique.
{"title":"M.S. Tswett and the invention of chromatography","authors":"L. Ettre","doi":"10.1142/9781860949449_0005","DOIUrl":"https://doi.org/10.1142/9781860949449_0005","url":null,"abstract":"On the occasion of the centenary of the invention of chromatography, this month's Milestones in Chromatography column chronicles the life and activities of its inventor, his struggle for acceptance, and, finally, the triumph of his technique.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"21 1","pages":"458-467"},"PeriodicalIF":0.9,"publicationDate":"2008-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/9781860949449_0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64008118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-06-01DOI: 10.1142/9781860949449_0029
L. Ettre
This month's installment of Milestones in Chromatography deals with the involvement of James L. Waters in two major chromatography developments: the introduction of instrumentation for gel-permeation chromatography and the establishment of Waters Associates as the Liquid Chromatography People.
本月的《色谱学里程碑》将介绍James L. Waters在色谱学两大发展中的作用:凝胶渗透色谱仪的引入以及作为液相色谱人的Waters Associates的成立。
{"title":"Jim waters : The development of GPC and the first commercial HPLC instruments","authors":"L. Ettre","doi":"10.1142/9781860949449_0029","DOIUrl":"https://doi.org/10.1142/9781860949449_0029","url":null,"abstract":"This month's installment of Milestones in Chromatography deals with the involvement of James L. Waters in two major chromatography developments: the introduction of instrumentation for gel-permeation chromatography and the establishment of Waters Associates as the Liquid Chromatography People.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"23 1","pages":"101-106"},"PeriodicalIF":0.9,"publicationDate":"2008-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64008755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2006-01-01DOI: 10.1142/9781860949449_0019
L. Ettre
This installment of “Millestones in Chromatography”discusses the events leading to the development of the amino acid analyzer near the end of the 1950s at The Rockefeller Institute of Medical Research by Moore, Stein, and Spackman.
{"title":"The development of the amino acid analyzer","authors":"L. Ettre","doi":"10.1142/9781860949449_0019","DOIUrl":"https://doi.org/10.1142/9781860949449_0019","url":null,"abstract":"This installment of “Millestones in Chromatography”discusses the events leading to the development of the amino acid analyzer near the end of the 1950s at The Rockefeller Institute of Medical Research by Moore, Stein, and Spackman.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"37 1","pages":"390-400"},"PeriodicalIF":0.9,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64008245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Over the course of a drug product's life (as much as 15-20 years or more) many significant advances in technology will occur. Implementing new analytical techniques (for example, capillary electrophoresis), or advances in existing technology (for example, sub-2-μm liquid chromatography [LC]) can significantly impact business practices (return on investment) and product quality. However, in a regulated laboratory, implementing new technology can present many challenges.
{"title":"Implementing New Technology in a Regulated Environment","authors":"M. Swartz, I. Krull","doi":"10.1201/B12039-10","DOIUrl":"https://doi.org/10.1201/B12039-10","url":null,"abstract":"Over the course of a drug product's life (as much as 15-20 years or more) many significant advances in technology will occur. Implementing new analytical techniques (for example, capillary electrophoresis), or advances in existing technology (for example, sub-2-μm liquid chromatography [LC]) can significantly impact business practices (return on investment) and product quality. However, in a regulated laboratory, implementing new technology can present many challenges.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"24 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1201/B12039-10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65975166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2005-01-01DOI: 10.1021/cen-v083n023.obc
Fang Xia, Jie Y. Cavanaugh, D. Diehl
SunFire C 18 analytical and OBD preparative columns are designed to provide maximum loadability in simple mobile phase conditions, accurate scalability, and high peak capacity.
SunFire C 18分析和OBD制备柱设计用于在简单的流动相条件下提供最大的负载性，准确的可扩展性和高峰值容量。
{"title":"Bridging the performance gap from analytical to preparative chromatography : Efficient target isolation from a complex mixture","authors":"Fang Xia, Jie Y. Cavanaugh, D. Diehl","doi":"10.1021/cen-v083n023.obc","DOIUrl":"https://doi.org/10.1021/cen-v083n023.obc","url":null,"abstract":"SunFire C 18 analytical and OBD preparative columns are designed to provide maximum loadability in simple mobile phase conditions, accurate scalability, and high peak capacity.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"1 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1021/cen-v083n023.obc","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57657555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1142/9781860949449_0021
L. Ettre
A special type of instrument that permitted the volume of collected fractions to be read directly was very popular in the early days of gas chromatography. This Milestones in Chromatography column deals with the development and applications of this long-forgotten unit.
{"title":"The Janák-type gas chromatographs of the 1950s","authors":"L. Ettre","doi":"10.1142/9781860949449_0021","DOIUrl":"https://doi.org/10.1142/9781860949449_0021","url":null,"abstract":"A special type of instrument that permitted the volume of collected fractions to be read directly was very popular in the early days of gas chromatography. This Milestones in Chromatography column deals with the development and applications of this long-forgotten unit.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":"20 1","pages":"866-874"},"PeriodicalIF":0.9,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64008478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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