Pub Date : 2023-10-01DOI: 10.56530/lcgc.na.ub5690a5
Tony Taylor
The author discusses the benefits and challenges of using hydrogen in the laboratory, specifically regarding gas chromatography and GC–MS procedures.
作者讨论了在实验室中使用氢的好处和挑战，特别是关于气相色谱和GC-MS程序。
{"title":"Health and Safety Concerns of Hydrogen Use with Gas Chromatography","authors":"Tony Taylor","doi":"10.56530/lcgc.na.ub5690a5","DOIUrl":"https://doi.org/10.56530/lcgc.na.ub5690a5","url":null,"abstract":"The author discusses the benefits and challenges of using hydrogen in the laboratory, specifically regarding gas chromatography and GC–MS procedures.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.56530/lcgc.na.un1878g5
Leon Doneski, David Roos, Michael Dong
Good Laboratory Practice (GLP) is a regulatory requirement to help ensure the quality, reliability, and integrity of pharmacological and toxicological studies during drug development. This article overviews GLP regulations and related public standards, focusing on practices relevant to analytical and bioanalytical chemists.
{"title":"Good Laboratory Practice (GLP): An Overview for the Analytical Chemist","authors":"Leon Doneski, David Roos, Michael Dong","doi":"10.56530/lcgc.na.un1878g5","DOIUrl":"https://doi.org/10.56530/lcgc.na.un1878g5","url":null,"abstract":"Good Laboratory Practice (GLP) is a regulatory requirement to help ensure the quality, reliability, and integrity of pharmacological and toxicological studies during drug development. This article overviews GLP regulations and related public standards, focusing on practices relevant to analytical and bioanalytical chemists.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.56530/lcgc.na.cy7988u5
Dwight R. Stoll
As chromatography technologies change, our approaches to using them in ways that value reliability, as well as the ways we should approach troubleshooting and fixing problems, should also change. In this installment, I continue the discussion started last month about how some specific troubleshooting topics have evolved over time, this time focusing on mobile phase degassing. Understanding how approaches to degassing have evolved is helpful for troubleshooting problems with pumps and detection, designing reliability into new methods, and when considering updates to legacy methods that have been in use for decades.
{"title":"The Evolution of LC Troubleshooting: Degassing","authors":"Dwight R. Stoll","doi":"10.56530/lcgc.na.cy7988u5","DOIUrl":"https://doi.org/10.56530/lcgc.na.cy7988u5","url":null,"abstract":"As chromatography technologies change, our approaches to using them in ways that value reliability, as well as the ways we should approach troubleshooting and fixing problems, should also change. In this installment, I continue the discussion started last month about how some specific troubleshooting topics have evolved over time, this time focusing on mobile phase degassing. Understanding how approaches to degassing have evolved is helpful for troubleshooting problems with pumps and detection, designing reliability into new methods, and when considering updates to legacy methods that have been in use for decades.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.56530/lcgc.na.tu5672b5
Douglas E. Raynie
This installment of “Sample Prep Perspectives” reports the results obtained from a new survey on sample preparation techniques. This is our first survey since 2016 and its findings are compared with the results of previous surveys dating from 1991. Results involve undercover trends in the field over the last generation and their impact. The survey investigated trends in areas such as which technologies are currently being used, sample loads, sample sizes, automation, the use of solid-phase extraction (SPE) devices (cartridges, disks, plates, tips), SPE chemistries, selection criteria, and problems encountered. Respondents were also asked about sample preparation technologies on the horizon.
{"title":"Trends in Sample Preparation, Part I: Current State of the Field","authors":"Douglas E. Raynie","doi":"10.56530/lcgc.na.tu5672b5","DOIUrl":"https://doi.org/10.56530/lcgc.na.tu5672b5","url":null,"abstract":"This installment of “Sample Prep Perspectives” reports the results obtained from a new survey on sample preparation techniques. This is our first survey since 2016 and its findings are compared with the results of previous surveys dating from 1991. Results involve undercover trends in the field over the last generation and their impact. The survey investigated trends in areas such as which technologies are currently being used, sample loads, sample sizes, automation, the use of solid-phase extraction (SPE) devices (cartridges, disks, plates, tips), SPE chemistries, selection criteria, and problems encountered. Respondents were also asked about sample preparation technologies on the horizon.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.56530/lcgc.na.fz8280l1
Mark Lies
Complex multispecific antibodies overcome the limitations of standard monoclonal antibodies (mAbs), and have complex three-dimensional structures, greater heterogeneity, and often novel disulfide linkages and unusual post-translational modifications. As a result, the comprehensive characterization essential to the development of cost-effective manufacturing processes and safe and efficacious cancer treatments is much more challenging using traditional analytical instruments and techniques. Fortunately, there are new solutions available that provide fast results, often with greater sensitivity and resolution, even for these novel, complex molecules. The application of various new methods and instruments for the analysis of multispecifics is discussed as a means of highlighting their ability to facilitate the development of these important new modalities.
{"title":"Overcoming Complex Analytical Challenges Posed by Targeted Multispecific Antibody Cancer Therapeutics","authors":"Mark Lies","doi":"10.56530/lcgc.na.fz8280l1","DOIUrl":"https://doi.org/10.56530/lcgc.na.fz8280l1","url":null,"abstract":"Complex multispecific antibodies overcome the limitations of standard monoclonal antibodies (mAbs), and have complex three-dimensional structures, greater heterogeneity, and often novel disulfide linkages and unusual post-translational modifications. As a result, the comprehensive characterization essential to the development of cost-effective manufacturing processes and safe and efficacious cancer treatments is much more challenging using traditional analytical instruments and techniques. Fortunately, there are new solutions available that provide fast results, often with greater sensitivity and resolution, even for these novel, complex molecules. The application of various new methods and instruments for the analysis of multispecifics is discussed as a means of highlighting their ability to facilitate the development of these important new modalities.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.zy5775b7
Nicholas H. Snow
In the past two decades, comprehensive two-dimensional gas chromatography (GC×GC) has progressed from an interesting concept to the forefront of thinking and research in gas chromatography. Especially when combined with mass spectrometry detection, GC×GC provides high resolution, high sensitivity, and massive chromatographic data sets, which is useful in diverse fields such as petroleum analysis, metabolomics, food, flavor, fragrance, and environmental analysis. In this installment, recent developments in GC×GC that make it more amenable for routine use are discussed. These include advances in instrumentation, especially modulation, column sets, data analysis, and the range and types of samples amenable to GC×GC. We will ponder the question – are you ready to make the switch to GC×GC?
{"title":"Are You Ready to Switch to Comprehensive Two-Dimensional Gas Chromatography?","authors":"Nicholas H. Snow","doi":"10.56530/lcgc.na.zy5775b7","DOIUrl":"https://doi.org/10.56530/lcgc.na.zy5775b7","url":null,"abstract":"In the past two decades, comprehensive two-dimensional gas chromatography (GC×GC) has progressed from an interesting concept to the forefront of thinking and research in gas chromatography. Especially when combined with mass spectrometry detection, GC×GC provides high resolution, high sensitivity, and massive chromatographic data sets, which is useful in diverse fields such as petroleum analysis, metabolomics, food, flavor, fragrance, and environmental analysis. In this installment, recent developments in GC×GC that make it more amenable for routine use are discussed. These include advances in instrumentation, especially modulation, column sets, data analysis, and the range and types of samples amenable to GC×GC. We will ponder the question – are you ready to make the switch to GC×GC?","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.vn3175v1
Steve Suh, David Kennedy, Reika Takahara, Manabu Takayanagi, Hiroshi Hayashida, Zhonghua Shen
The authors evaluated a sampling and analytical system to measure differing modes of atmospheric per- and polyfluorinated substances (PFAS) transmission. The sampling was conducted with the FM4 sampling module, which features particulate collection followed by polyurethane foam sorption followed by activated carbon disc adsorption, a configuration designed to capture PFAS-laden particulate matter (one-micron to ten-microns), as well as aerosol, volatile, and ionic PFAS species in a single sampling event. Individual sampling media fractions were subsequently analyzed by gas chromatography coupled with tandem quadrupole mass spectrometry (GC–MS/MS) and liquid chromatography coupled with tandem quadrupole mass spectrometry (LC–MS/MS) for 33 ionic and 20 neutral PFAS species. A multi-day field sampling event conducted in an outdoor urban environment demonstrated that the system was able to capture and differentiate a number of airborne PFAS species. The study showed that simultaneous, multi-phase sample collection coupled with GC–MS/MS and LC–MS/MS analysis can be a useful approach to further elucidate the mode and manner of atmospheric PFAS transmission.
{"title":"A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS Transmission","authors":"Steve Suh, David Kennedy, Reika Takahara, Manabu Takayanagi, Hiroshi Hayashida, Zhonghua Shen","doi":"10.56530/lcgc.na.vn3175v1","DOIUrl":"https://doi.org/10.56530/lcgc.na.vn3175v1","url":null,"abstract":"The authors evaluated a sampling and analytical system to measure differing modes of atmospheric per- and polyfluorinated substances (PFAS) transmission. The sampling was conducted with the FM4 sampling module, which features particulate collection followed by polyurethane foam sorption followed by activated carbon disc adsorption, a configuration designed to capture PFAS-laden particulate matter (one-micron to ten-microns), as well as aerosol, volatile, and ionic PFAS species in a single sampling event. Individual sampling media fractions were subsequently analyzed by gas chromatography coupled with tandem quadrupole mass spectrometry (GC–MS/MS) and liquid chromatography coupled with tandem quadrupole mass spectrometry (LC–MS/MS) for 33 ionic and 20 neutral PFAS species. A multi-day field sampling event conducted in an outdoor urban environment demonstrated that the system was able to capture and differentiate a number of airborne PFAS species. The study showed that simultaneous, multi-phase sample collection coupled with GC–MS/MS and LC–MS/MS analysis can be a useful approach to further elucidate the mode and manner of atmospheric PFAS transmission.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135588500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.hv2690i5
Xiaomeng Liang, Jan H. Christensen, Thomas D. Bucheli, Nikoline Juul Nielsen
Phytotoxins, secondary metabolites synthesized by plants, are now recognized as a new category of environmental micropollutants. Only a limited number of phytotoxins have been detected and reported in terrestrial and aquatic environments, partially due to analytical challenges. In this article, a robust, reliable, and efficient approach, namely Source Supported Suspect Screening (4S), is introduced for high-throughput analysis of phytotoxins from their plant origin to the downstream environmental compartments. The approach was established on an ultrahigh-performance liquid chromatography–electrospray ionization–quadrupole‑time‑of‑flight-mass spectrometry (UHPLC–ESI–QTOF-MS) analytical platform where optimal sample preparation and chromatographic conditions were investigated and a new suspect screening protocol was suggested.
{"title":"Suspect Screening of Lupin-Produced Phytotoxins in Environmental Samples","authors":"Xiaomeng Liang, Jan H. Christensen, Thomas D. Bucheli, Nikoline Juul Nielsen","doi":"10.56530/lcgc.na.hv2690i5","DOIUrl":"https://doi.org/10.56530/lcgc.na.hv2690i5","url":null,"abstract":"Phytotoxins, secondary metabolites synthesized by plants, are now recognized as a new category of environmental micropollutants. Only a limited number of phytotoxins have been detected and reported in terrestrial and aquatic environments, partially due to analytical challenges. In this article, a robust, reliable, and efficient approach, namely Source Supported Suspect Screening (4S), is introduced for high-throughput analysis of phytotoxins from their plant origin to the downstream environmental compartments. The approach was established on an ultrahigh-performance liquid chromatography–electrospray ionization–quadrupole‑time‑of‑flight-mass spectrometry (UHPLC–ESI–QTOF-MS) analytical platform where optimal sample preparation and chromatographic conditions were investigated and a new suspect screening protocol was suggested.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.ld1067s1
Ron A. H. Peters
The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.
讨论了用液相色谱法分析共聚物的最新进展和面临的挑战。
{"title":"Functional Copolymers Analysis Using Liquid Chromatography (LC)—Sort It Out","authors":"Ron A. H. Peters","doi":"10.56530/lcgc.na.ld1067s1","DOIUrl":"https://doi.org/10.56530/lcgc.na.ld1067s1","url":null,"abstract":"The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.56530/lcgc.na.hq8476g7
David S. Bell
The 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023), chaired by Michael Lämmerhofer and Oliver Schmitz, was held from June 18 to 22 in Duesseldorf, Germany. This installment of “Column Watch” presents many of the highlighted topics and trends observed at this exceptionally well-run symposium.
{"title":"Highlights from the 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023)","authors":"David S. Bell","doi":"10.56530/lcgc.na.hq8476g7","DOIUrl":"https://doi.org/10.56530/lcgc.na.hq8476g7","url":null,"abstract":"The 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023), chaired by Michael Lämmerhofer and Oliver Schmitz, was held from June 18 to 22 in Duesseldorf, Germany. This installment of “Column Watch” presents many of the highlighted topics and trends observed at this exceptionally well-run symposium.","PeriodicalId":17992,"journal":{"name":"Lc Gc North America","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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