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Newly Recorded Panus lecomtei (Fr.) Corner (Basidiomycota: Panaceae) for Indonesia 印度尼西亚新记录Panus lecomtei (Fr.) Corner(担子菌科:Panaceae)
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1507
Panus lecomtei (Polyporales) is reported for the first time from Indonesia at the IPB University Campus Forest (IPBUCF). This species has similar macroscopic features to P. strigellus and P. neostrigosus. This study aimed to confirm the taxonomic position of P. lecomtei found at IPBUCF based on morphological and molecular evidence. The specimens were described by their macroscopic and microscopic characteristics. A phylogenetic approach was used with the internal transcribed spacer 1/2 sequence data. The combination of morphological and molecular analyses confirmed the taxonomic position of our specimens as P. lecomtei. The recorded species was distinguished by gregarious basidiomata, an infundibuliform resembling a funnel, violet during the immature stage to orangish brown in the mature pileus and ellipsoid basidiospore stages. Our specimens were distinguished from P. strigellus by having smaller basidiospores. It was difficult to separate P. lecomtei BO24428 from P. neostrigosus based on morphological characters. However, the BLAST results showed that specimen BO24428 had 99.56% similarity with P. lecomtei. In addition, our phylogenetic tree nested P. lecomtei BO24428 in the P. lecomtei clade. Morphological illustrations and a phylogenetic tree of P. lecomtei are provided. We suggest combining the morphological and molecular evidence in future studies of P. lecomtei from Indonesia
在IPB大学校园森林(IPBUCF)首次报道了印度尼西亚的Panus lecomtei (Polyporales)。本种具有相似的宏观特征,以假单胞菌和新假单胞菌。本研究旨在根据形态学和分子生物学的证据,确定在IPBUCF发现的lecomtei的分类地位。通过宏观和微观特征对试样进行了描述。系统发育方法用于内部转录间隔物1/2序列数据。形态分析和分子分析的结合证实了我们的标本在分类上的地位是lecomtei。所记录的物种以群居的担子孢子为特征,呈漏斗状,在未成熟阶段呈紫色,在成熟的毛状和椭球状担子孢子阶段呈橙黄色。我们的标本是由具有较小的担子孢子,以区别于P. strigellus。根据形态特征,很难将P. lecomtei BO24428与P. neostrigosus区分开来。BLAST结果显示,BO24428标本与P. lecomtei的相似性为99.56%。此外,我们的系统发育树将P. lecomtei BO24428嵌套在P. lecomtei分支中。本文提供了水蛭的形态图和系统发育树。我们建议在今后的研究中结合形态学和分子学的证据
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引用次数: 0
Effect of Calorie Restriction on the Expression of Sirtuin1 as an Antiaging Biomarker 热量限制对抗衰老生物标志物Sirtuin1表达的影响
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1529
Calorie restriction (CR) is the most effective method for delaying aging and preventing the onset of age-related diseases. Sirtuins constitute a family of nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylases. Their activity can be regulated by NAD+/NADH levels, which are influenced by nutrient intake, a variable acted upon by CR. This review elaborates on the link between CR and sirtuin1 (SIRT1). It retrieved articles from several sources, such as ClinicalKey, PubMed, and ScienceDirect. It discusses the up-to-date knowledge of how SIRT1 acts as a nutrient sensor and regulator of molecular mechanisms. These mechanisms include the control of the cell cycle, enhancing mitochondrial quality control, activating fatty acid oxidation, and stimulating anti-inflammatory effects. Disruptions in the aforementioned mechanisms are the basis of aging. CR increases the expression of SIRT1, which enhances the biogenesis and dynamics of mitochondria, resulting in an antiaging effect. In CR, SIRT1 is activated and stimulates different pathways, especially those related to mitochondrial activity and effectiveness, leading to an antiaging effect in collaboration with other antiaging biomarkers
热量限制(CR)是延缓衰老和预防与年龄有关的疾病发病的最有效方法。Sirtuins构成了一个烟酰胺腺嘌呤二核苷酸(NAD)依赖性组蛋白去乙酰化酶家族。它们的活性可受NAD+/NADH水平的调控,而NAD+/NADH水平受营养摄入的影响,而营养摄入又受CR的影响。本文详细阐述了CR与SIRT1之间的联系。它从几个来源检索文章,如ClinicalKey、PubMed和ScienceDirect。它讨论了SIRT1如何作为营养传感器和分子机制调节器的最新知识。这些机制包括控制细胞周期、加强线粒体质量控制、激活脂肪酸氧化和刺激抗炎作用。上述机制的破坏是衰老的基础。CR增加SIRT1的表达,从而增强线粒体的生物发生和动力学,从而达到抗衰老的作用。在CR中,SIRT1被激活并刺激不同的通路,特别是与线粒体活性和有效性相关的通路,从而与其他抗衰老生物标志物协同发挥抗衰老作用
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引用次数: 0
Antibacterial Testing on Silver/Zinc Oxide Nanoparticles/Organoclay Reinforced Chitosan Biocomposites 纳米银/氧化锌/有机粘土增强壳聚糖生物复合材料的抗菌性能研究
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.2114
Herein, bionanocomposites of chitosan (CS)/silver nanoparticle/organoclay/zinc oxide nanoparticle (CS/Ag/OC/ZnO) were prepared for antibacterial food packaging. This study examines the time variation in the AgNP synthesis method by comparing local (74˗85% deacetylated) and commercial chitosan (75%˗85% deacetylated) as a reducing and capping agent and seeks to reconstruct the optimum ratio formulations of AgNPs and ZnONPs in bionanocomposites for food packaging. The results reveal that the synthesis of AgNPs was successfully carried out using a local chitosan solution as a reducing and capping agent. The CS/Ag/OC/ZnO films exhibit structural, mechanical, and optical properties suitable for food packaging and antibacterial activity on Staphylococcus aureus and Escherichia coli. The relative inhibition zone increased with increasing numbers of AgNPs and ZnONPs. The inhibition zone values indicated that E. coli and S. aureus bacteria were sensitive to the film, namely 12.5 ± 1.5 mm, and 16 ± 0.0 mm, respectively.
制备了壳聚糖(CS)/纳米银颗粒/有机粘土/纳米氧化锌颗粒(CS/Ag/OC/ZnO)的生物纳米复合材料,用于抗菌食品包装。本研究通过比较本地(74 - 85%去乙酰化)壳聚糖和商业(75% - 85%去乙酰化)壳聚糖作为还原剂和旋盖剂,考察AgNP合成方法的时间变化,并试图重建用于食品包装的生物纳米复合材料中AgNP和ZnONPs的最佳配比配方。结果表明,用局部壳聚糖溶液作为还原封盖剂,成功地合成了AgNPs。CS/Ag/OC/ZnO薄膜具有适合食品包装的结构、力学和光学性能,并具有对金黄色葡萄球菌和大肠杆菌的抑菌活性。相对抑制区随着AgNPs和ZnONPs数量的增加而增大。抑菌带值表明,大肠杆菌和金黄色葡萄球菌对膜敏感,分别为12.5±1.5 mm和16±0.0 mm。
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引用次数: 0
Synthesis, Characterization, Antibacterial Activity, and Potential Water Filter Application of Copper Oxide/Zeolite Composite 氧化铜/沸石复合材料的合成、表征、抗菌活性及潜在的水过滤应用
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1555
Detection of E. coli bacteria in water samples can indicate the presence of other bacterial contamination in feces. Bacterial contamination can be treated with antibiotics, but excessive use of antibiotics leads to the emergence of multiresistant bacteria. Therefore, alternative antibacterial agents must be explored. Copper ion/zeolite composite has been widely studied as an antibacterial agent. However, the released copper ions accumulate in water and are toxic to humans; this can be prevented by converting copper ions into copper oxides (CuO and Cu2O). In this study, copper oxide/zeolite composite is synthesized and characterized using the heating method, followed by evaluation of its effect, antibacterial activity, and potential application as a water filter. The results showed that heat treatment and combination of zeolite with copper did not change the composition of zeolite, rather impurities were reduced from natural zeolites. Antibacterial activity test against E. coli showed that the copper oxide/zeolite composite possessed good antibacterial activity and exhibited better potential as a water filter compared with copper/zeolite. The copper ion released from the synthesized materials was under 2 mg/L, indicating that it is safe for use provided the minimum contact between the sample and water for less than 20 min or 50–60 min.
在水样中检测出大肠杆菌可以表明粪便中存在其他细菌污染。细菌污染可以用抗生素治疗,但过度使用抗生素会导致多重耐药细菌的出现。因此,必须探索替代抗菌剂。铜离子/沸石复合材料作为一种抗菌剂得到了广泛的研究。然而,释放出来的铜离子在水中积聚,对人体有毒;这可以通过将铜离子转化为铜氧化物(CuO和Cu2O)来防止。本研究采用加热法合成了氧化铜/沸石复合材料,并对其进行了表征,对其效果、抗菌活性以及作为水过滤器的潜在应用进行了评价。结果表明:热处理及沸石与铜的结合没有改变沸石的组成,反而减少了天然沸石中的杂质。对大肠杆菌的抑菌活性试验表明,氧化铜/沸石复合材料具有良好的抑菌活性,与铜/沸石相比,具有更好的水过滤潜力。从合成材料中释放出的铜离子低于2 mg/L,表明只要样品与水的最小接触时间不超过20分钟或50-60分钟,就可以安全使用。
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引用次数: 0
In-silico Approach for Predicting the Inhibitory Effect of Home Remedies on Severe Acute Respiratory Syndrome Coronavirus-2 家用偏方对严重急性呼吸综合征冠状病毒-2抑制效果的计算机预测方法
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1609
Severe Acute Respiratory Syndrome Coronavirus-2 is an infectious disease caused by coronavirus and spreads through infected droplets inhalation. For the past 3 years, COVID-19 has become a global threat. Vaccinations are currently available and have FDA approval. During the recent pandemic, people adopted a psychological belief that home remedies (turmeric, ginger, garlic, and coriander) can prevent COVID-19. This research aimed to predict the covid-19 inhibitory activities by home remedies through an in-silico approach. The 24 bioactive constituents of four home remedies plants were downloaded from PubChem and subjected to molecular docking with the three important proteins responsible for COVID-19 pathogenesis. The absorption, distribution, metabolism, and excretion (ADME) properties and toxicity of the constituents were also predicted with ADMETlab and ProTox-II software. Docking was performed with AutoDock Vina 1.5.7, and molecular interaction was visualized by Discovery studio visualizer 2021. In terms of binding energy, the active constituent present in turmeric powder (curcumin) showed the best binding interaction of ΔG = −6.8, 8.5, 8.7 kcal/mol with three different proteins Mpro (6LU7), S protein (6VXX), and ACE2 receptor protein (7P19), respectively. All the active constituents of turmeric powder have no toxicity and have suitable ADME properties. Along with curcumin and its derivatives, quercetin as an active constituent of garlic showed the best Covid-19 inhibitory effect with no toxicity. In conclusion, home remedies can prevent Covid-19 infection. In vitro animal study is required to validate these data
冠状病毒-2是一种由冠状病毒引起的传染病,通过吸入受感染的飞沫传播。过去三年来,COVID-19已成为全球威胁。疫苗目前是可用的,并已获得FDA的批准。在最近的大流行期间,人们在心理上相信家庭疗法(姜黄、生姜、大蒜和香菜)可以预防COVID-19。本研究旨在通过计算机方法预测家庭偏方对covid-19的抑制活性。从PubChem中下载4种家药植物的24种生物活性成分,并与3种重要的COVID-19致病蛋白进行分子对接。利用ADMETlab和ProTox-II软件预测各成分的吸收、分布、代谢和排泄(ADME)特性和毒性。对接工具为AutoDock Vina 1.5.7,分子相互作用可视化工具为Discovery studio visualizer 2021。结合能方面,姜黄粉中的活性成分姜黄素与3种不同蛋白Mpro (6LU7)、S蛋白(6VXX)和ACE2受体蛋白(7P19)的结合能分别为ΔG =−6.8、8.5、8.7 kcal/mol。姜黄粉的所有活性成分均无毒,具有合适的ADME性能。大蒜活性成分槲皮素与姜黄素及其衍生物对新冠病毒的抑制效果最好,且无毒性。总之,家庭疗法可以预防Covid-19感染。需要体外动物研究来验证这些数据
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引用次数: 1
Fabrication and Characterization of Nylon 6 Fiber via Wet Spinning Method for Application as a Reinforcing Material for a Direct Dental Bridge 用湿纺丝法制备尼龙6纤维用于直接牙桥的增强材料及其性能
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1397
Nylon is a biocompatible thermoplastic polymer that is well known for its excellent fracture resistance, making it suitable for fabricating fiber-reinforced composite (FRC)-based dental bridges. FRC is widely used in dentistry. This study aimed to investigate the structural and morphological characteristics of the nylon 6 fiber prepared using five different weights of nylon 6 dissolved in formic acid. The nylon 6 fiber was successfully fabricated via a simple wet spinning method using water as a coagulant. The fiber was then characterized using Fourier transform infrared (FTIR) spectroscopy, optical microscopy, and scanning electron microscopy. FTIR spectroscopy confirmed the presence of nylon 6 characteristics in the fiber in the form of N–H and C=O groups at a specific wave number. The differences in the diameter and morphological shape of the fiber were attributed to the nylon 6 different concentrations. Furthermore, the nylon 6 fiber can be used to produce cost-effective products and realize suitable characteristics for use as an alternative to traditional materials for fabricating direct dental bridges.
尼龙是一种生物相容性热塑性聚合物,以其优异的抗断裂性而闻名,适用于制造纤维增强复合材料(FRC)基牙桥。FRC广泛应用于牙科。研究了五种不同重量的尼龙6在甲酸溶液中溶解制备的尼龙6纤维的结构形态特征。以水为混凝剂,采用简单的湿纺法成功制备了尼龙6纤维。然后使用傅里叶变换红外光谱(FTIR),光学显微镜和扫描电子显微镜对纤维进行了表征。FTIR光谱证实尼龙6在特定波数下以N-H和C=O基团的形式存在于纤维中。尼龙6浓度的不同导致了纤维直径和形态的差异。此外,尼龙6纤维可用于生产具有成本效益的产品,并实现作为传统材料的替代品用于制造直接牙桥的合适特性。
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引用次数: 0
Isolation of Lignin from Sugarcane Bagasse as an Adsorbent for Chromium Ion 蔗渣中木质素作为铬离子吸附剂的分离研究
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1446
The waste generated in metal coating and leather tanning industries contribute to water pollution owing to the use of Cr metal in production processes. The utilization of lignin from natural base materials in the form of bagasse can reduce unwanted waste from production processes and adsorb Cr ion waste. In this study, lignin was successfully isolated from bagasse waste and then carbonized and applied in Cr metal absorption. Lignin and lignin carbon as an absorbent were characterized using Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The ability of bagasse lignin and carbon lignin to absorb Cr ion waste was evaluated by monitoring mass fluctuation, contact time, and pH. The optimal conditions for adsorption were determined as follows: 0.015 g, 90 min, and pH 6. The adsorption isotherm followed the Freundlich model, and the adsorption kinetics followed the pseudo-first-order model. Furthermore, adsorption thermodynamics showed that the reaction proceeded spontaneously, and the disorder degree increased in the adsorption system
由于在生产过程中使用金属铬,金属涂层和皮革制革工业产生的废物造成了水污染。利用甘蔗渣形式的天然基础材料中的木质素可以减少生产过程中产生的不需要的废物,并吸附铬废物。本研究成功地从甘蔗渣中分离出木质素,并进行炭化处理,将木质素用于金属铬的吸附。利用傅里叶变换红外光谱(FTIR)和扫描电镜(SEM)对木质素和木质素碳作为吸附剂进行了表征。通过监测质量波动、接触时间和pH来评价蔗渣木质素和碳木质素对废铬离子的吸附能力,确定最佳吸附条件为:0.015 g, 90 min, pH 6。吸附等温线符合Freundlich模型,吸附动力学符合准一级模型。吸附热力学结果表明,反应自发进行,吸附体系的无序度增大
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引用次数: 0
Pro-Apoptosis Activity of Pogostemon cablin Benth. Against Nasopharyngeal Carcinoma through the BCL-2 Inhibition Signaling Pathway: A Computational Investigation 广藿香促细胞凋亡活性研究。通过BCL-2抑制信号通路抗鼻咽癌的计算研究
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1484
Resistance to chemotherapy and radiotherapy frequently emerges in the later stage of nasopharyngeal carcinoma (NPC) tumorigenesis. The decreased response of NPC to radiotherapy and chemotherapy occurs owing to the inhibition of cancer cell apoptosis by the B-cell lymphoma-2 (BCL-2) protein. Thus, inhibiting BCL-2 protein may become a powerful approach to eliminate NPC through apoptosis regulation. Meanwhile, Pogostemon cablin is reported to exhibit anticancer properties, but there are limited studies on its use for NPC treatment. The objective of this study is to investigate the potential bioactive compounds in P. cablin as anti-apoptosis BCL-2 protein inhibitors using in-silico approach. Natural compounds from P. cablin were retrieved from the KNApSAcK database and screened for inhibitory effects on BCL-2 protein via molecular docking coupled with molecular dynamics. It was found that apigenin, rhamnetin, and apigenin 7-(6″-p coumarylglucoside) showed potential inhibitory properties against BCL-2 protein based on binding affinity and interaction chemistry. The highest binding affinity was recorded for apigenin 7-(6″-p coumarylglucoside) at −9.9 kcal/mol, followed by rhamnetin and apigenin at −7.2 kcal/mol. These compounds are also bound to the inhibitory sites of BCL-2 and venetoclax, mainly by hydrophobic bonds and Van der Waals interactions. Nevertheless, molecular dynamics simulations revealed that apigenin 7-(6″-p-coumarylglucoside) had unstable conformation and binding to BCL-2. In summary, this study demonstrated that P. cablin has excellent potency as an alternative or complementary therapy against radiotherapy and chemotherapy resistance of NPC, mainly through rhamnetin and apigenin.
在鼻咽癌发生的晚期,经常出现化疗和放疗的耐药。由于b细胞淋巴瘤-2 (BCL-2)蛋白抑制癌细胞凋亡,鼻咽癌对放疗和化疗的反应降低。因此,抑制BCL-2蛋白可能成为通过细胞凋亡调控消除鼻咽癌的有效途径。同时,据报道广藿香具有抗癌特性,但其用于鼻咽癌治疗的研究有限。本研究的目的是利用计算机模拟方法,研究紫菜中潜在的生物活性化合物作为抗凋亡BCL-2蛋白抑制剂。从KNApSAcK数据库中检索cablin的天然化合物,通过分子对接结合分子动力学方法筛选其对BCL-2蛋白的抑制作用。结果表明,芹菜素、鼠李素和芹菜素7-(6″-p coumarylglucoside)对BCL-2蛋白具有潜在的抑制作用。芹菜素7-(6″-p香豆素糖苷)的结合亲和力最高,为- 9.9 kcal/mol,其次是鼠李素和芹菜素,为- 7.2 kcal/mol。这些化合物主要通过疏水键和范德华相互作用与BCL-2和venetoclax的抑制位点结合。然而,分子动力学模拟表明,芹菜素7-(6″-p-coumarylglucoside)具有不稳定的构象,并与BCL-2结合。综上所述,本研究表明,P. cablin主要通过鼠李糖素和芹菜素作为鼻咽癌放疗和化疗耐药的替代或补充疗法具有良好的疗效。
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引用次数: 0
Synthesis of Copper (II) Oxide Nanoparticles using Vitis vinifera L. Leaf Ex-tract and its Application as a Catalyst in Doebner Reaction 葡萄叶提取物合成氧化铜纳米颗粒及其在Doebner反应中的应用
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1442
Plant extracts can be used to synthesize nanoparticle because it is efficient, economical, and friendly to the environment. Copper oxide nanoparticles (CuO NPs) were synthesized using an aqueous fraction of Vitis vinifera L leaf extract and Cu(NO3)2 solution as a precursor. This fraction contains secondary metabolites as a reduction agent and stabilizing agents to formed CuO NPs. CuO NPs can be used as a catalyst in a chemical reaction, one of which is the Doebner reaction, which is a simple and efficient reaction of aniline, aromatic aldehydes, and pyruvic acid under reflux conditions to produce derivatives quinoline-4-carboxylic acid. CuO NPs were effectively used in the Doebner reaction and yielded a product is 79.84%. The CuO NPs characterized by Fourier Transform Infra-Red (FTIR), and X-ray Diffraction (XRD) which showed CuO NPs were formed with a size of 24.39 nm. The product synthesized was characterized by TLC (Thin Layer Chromatography), FTIR, UV-Vis Spectrophotometry, and Liquid Chromatography Mass Spectrometry (LC-MS) and confirmed 2-phenylquinoline-4-carboxylic acid was formed.
利用植物提取物制备纳米颗粒具有高效、经济、环保等优点。以葡萄叶提取物水溶液为原料,以Cu(NO3)2溶液为前驱体,合成了氧化铜纳米颗粒。该部分含有次级代谢物,作为还原剂和稳定剂形成CuO NPs。CuO NPs可以用作化学反应的催化剂,其中之一是Doebner反应,这是苯胺、芳香醛和丙酮酸在回流条件下生成衍生物喹啉-4-羧酸的简单而高效的反应。CuO NPs在Doebner反应中得到有效利用,产物收率为79.84%。傅里叶变换红外光谱(FTIR)和x射线衍射(XRD)表征表明,形成的CuO NPs尺寸为24.39 nm。通过薄层色谱(TLC)、红外光谱(FTIR)、紫外可见分光光度(UV-Vis)、液相色谱质谱(LC-MS)等手段对合成产物进行表征,证实产物为2-苯基喹啉-4-羧酸。
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引用次数: 0
Chicken Immune Responses to Vaccination by the Avian Influenza Subtype H5N1 against Avian Influenza 鸡对H5N1亚型禽流感疫苗的免疫反应
Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.7454/mss.v27i3.1367
Avian Influenza (AI) is one of the strategic animal diseases still considered a priority for control by the Indonesian government. This study aimed to examine the immune response of chickens to various types of H5N1 subtype AI vaccines in Indonesia and to determine the correlation of factors influencing the post-vaccination AI antibody response. Serum samples were tested using the hemagglutination inhibition (HI) test with the standard AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) and strain A/Chicken/Semarang/04141225-07/2014 (Clade 2.3.2). Antibody titer was calculated using the Geometric Mean Titer (GMT). The correlation analyses were performed to assess the correlation of antibody titer against each of the following factors: age of chicken at the time of sampling, the interval between sampling time and the previous vaccination, and the number of vaccinations performed. The results showed that the average antibody titer value against the AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) was highest in samples from the South Sumatra Province, namely, Palembang City, which was 26.42 HIU. The results showed a significant correlation (p-value <0,05) between antibody titer and the interval between sampling time and the previous vaccination, the number of vaccination performed and age of chicken at the time of sampling. Therefore, results showed that the immunity developed from vaccination using the AI vaccine seed subtype H5N1 could induce immunity with a protective value of ≥16.
禽流感是印度尼西亚政府仍然认为需要优先控制的战略动物疾病之一。本研究旨在检测印度尼西亚鸡对不同类型H5N1亚型禽流感疫苗的免疫反应,并确定影响疫苗接种后禽流感抗体反应的因素的相关性。血清样品采用血凝抑制(HI)试验,检测H5N1标准AI抗原亚型A/Chicken/Barru/BBVM/41-13/2013(进化枝2.1.3)和A/Chicken/Semarang/04141225-07/2014(进化枝2.3.2)。使用几何平均滴度(GMT)计算抗体滴度。进行相关分析以评估抗体滴度与以下各因素的相关性:采样时鸡的年龄、采样时间与前一次接种疫苗的间隔以及接种疫苗的次数。结果显示,来自南苏门答腊省巨港市的样本对禽流感抗原亚型H5N1 A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3)的平均抗体效价最高,为26.42 HIU。结果表明,抗体滴度与采样时间与前一次接种疫苗的间隔时间、接种疫苗的次数和采样时鸡龄呈显著相关(p值< 0.05)。由此可见,接种H5N1禽流感疫苗种子亚型后产生的免疫可诱导免疫,保护值≥16。
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引用次数: 0
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Makara Journal of Science
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