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Isolation, identification and antibiotic susceptibility of gram-negative anaerobic bacteria in oral cavity 口腔革兰氏阴性厌氧菌的分离鉴定及药敏分析
Pub Date : 1900-01-01 DOI: 10.2298/sgs2302087t
Z. Tambur, E. Aleksić, J. Milutinović, A. Malesevic, Dejana Subotic, V. Biočanin
Gram-negative bacteria include more than 20 genera. The most commonly isolated genera are: Bacteriodes spp., Porphiromonas spp., Fusobacterium spp. and Prevotella spp. The following genera were isolated somewhat less frequently: Tanerella spp., Leptotricha spp., Veilonella spp.,Wollinela spp., Selenomonas spp. and Treponema spp. Anaerobic bacteria have an anaerobic type of metabolism and therefore their incubation is significantly longer and more demanding than aerobic bacteria. The genera Prevotella spp., Porphyromonas spp. and Fusobacterium spp. are part of the resident flora of the oral cavity and in unfavorable conditions cause periodontal diseases, and sometimes dentogenic infections and systemic diseases, such as Alzheimer?s disease, cardiovascular diseases, metabolic diseases and inflammatory bowel diseases. The three most important steps for the successful diagnosis of anaerobic bacteria are: proper sampling with avoiding sample contamination, rapid transport of samples to the microbiological laboratory and proper handling of samples. A combination of beta-lactam with the addition of beta-lactamase inhibitors, metronidazole, clindamycin and moxifloxacin is used for treatment for infections caused by anaerobic Gram-negative bacteria. It is important to note that antibiotics should be used only with a clear indication and to choose the right antibiotic in the optimal dose. The aim of this review is to point out the role of Gram-negative anaerobic bacteria in periodontal diseases, and its isolation, identification and antibiotic susceptibility.
革兰氏阴性菌包括20多个属。最常见的分离属是:拟杆菌属、卟啉单胞菌属、梭杆菌属和普雷沃氏菌属。以下属的分离频率稍低:Tanerella spp.、Leptotricha spp.、Veilonella spp.、Wollinela spp.、Selenomonas spp.和密螺旋体spp.厌氧细菌具有厌氧代谢类型,因此它们的培养时间明显长于好氧细菌,要求更高。普氏菌属、卟啉单胞菌属和梭杆菌属是口腔常住菌群的一部分,在不利的条件下会引起牙周病,有时还会引起牙源性感染和全身性疾病,如阿尔茨海默病。S病、心血管疾病、代谢性疾病和炎症性肠病。成功诊断厌氧菌的三个最重要的步骤是:正确取样,避免样品污染,快速将样品运送到微生物实验室,正确处理样品。β -内酰胺与β -内酰胺酶抑制剂、甲硝唑、克林霉素和莫西沙星的组合用于治疗由厌氧革兰氏阴性菌引起的感染。重要的是要注意,抗生素只能在明确的适应症下使用,并选择正确的抗生素和最佳剂量。本文就革兰氏阴性厌氧菌在牙周病中的作用、分离鉴定及药敏研究进展作一综述。
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引用次数: 0
Dentin thickness of the mesio-buccal root wall of the lower first molar using cone beam computerized tomography (CBCT) 锥形束计算机断层扫描(CBCT)对下第一磨牙中颊根壁牙本质厚度的研究
Pub Date : 1900-01-01 DOI: 10.2298/sgs2302071d
Aleksandra Djeri, Irena Radman-Kuzmanović, Nikola Moravac, Milan Tejic, Renata Josipović, Adriana Arbutina, Saša Marin, N. Trtić, V. Veselinović
Introduction. The aim of this work was to check the average thickness of dentinal wall of the mesio-buccal root of the first lower molar using CBCT axial sections of intact teeth. Material and method. In this cross-sectional study, 100 CBCT images of mandibular first molars from the Promax 3Dent radiology center in Banja Luka, Bosnia and Herzegovina were evaluated. A Planmeca Promax3D Max apparatus (Planmeca, Helsinki, Finland) was used, with a field of view (FOV) of 8 ? 8 cm and a resolution of 0.1 mm. Analysis was performed using Romexis Viewer software version 3.1.1 (Planmeca, Helsinki, Finland). At an axial thickness of 0.1 mm, transverse sections with a distance of 1 mm were made below the furcation area (at a magnification ?10) at a distance of 1, 2, 3, 4, 5 mm from the furcation. The data were then analyzed by paired t-test. Results. The thickness of dentinal wall of the mesio-buccal root of the lower first molar was significantly smaller at 2 mm and 3 mm than when measured at 1, 4 and 5 mm (p < 0.05). There was no significant difference between measurements at 1, 4 and 5 mm below the furcation area (p = 0.001). Conclusion. Due to the small thickness of dentinal wall at 2-3 mm below the furcation in the root canal, caution is required when instrumenting mesio-buccal canal of the lower first molar and correct selection of the instrumentation technique in order to avoid transportation or stripping perforation that may lead to root fracture.
介绍。本研究的目的是利用完整牙的CBCT轴向切片检查第一下磨牙中颊根牙本质壁的平均厚度。材料和方法。在这项横断面研究中,对来自波斯尼亚和黑塞哥维那Banja Luka Promax 3Dent放射中心的下颌第一磨牙的100张CBCT图像进行了评估。使用Planmeca Promax3D Max设备(Planmeca,赫尔辛基,芬兰),视场(FOV)为8 ?8厘米,分辨率为0.1毫米。采用Romexis Viewer 3.1.1版软件(Planmeca, Helsinki, Finland)进行分析。在轴向厚度为0.1 mm时,在距离分叉1、2、3、4、5 mm处,在分叉区域下方(放大倍率为?10)做距离为1 mm的横切面。采用配对t检验对数据进行分析。结果。下第一磨牙中颊根牙管壁厚度在2、3 mm处明显小于1、4、5 mm处(p < 0.05)。在分叉区以下1,4,5 mm处的测量结果无显著差异(p = 0.001)。结论。由于根管分叉下2 ~ 3 mm的牙管壁厚度较小,在进行下第一磨牙中颊管内固定时要谨慎,正确选择固定技术,避免运输或剥离穿孔导致根管断裂。
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引用次数: 0
Cbct analysis of apical distance between second lower premolars, first and second lower molars and mandibular canal 第二下前磨牙、第一、第二下磨牙与下颌管尖距的Cbct分析
Pub Date : 1900-01-01 DOI: 10.2298/sgs2103122d
Aleksandra Djeri, Natasa Brestovac, S. Subotić, Irena Radman-Kuzmanović, Adriana Arbutina, Saša Marin
Introduction. Mandibular canal with the associated neurovascular bundle may be closely related to the apices of mandibular teeth. In order to avoid injuries and damages to the inferior alveolar nerve during invasive dental procedures, it is important to know its localization. The aim of this study was to determine the average vertical distances of the root apices of second premolars, first molars and second molars mesially and distally from the upper projection of the mandibular canal on the sagittal section of CBCT images, and determine if there were statistically significant differences between the age and gender groups. Material and methods. The research was conducted at the Faculty of Medicine of the University of Banja Luka, and the sample consisted of 146 CBCT images of patients. CBCT images were obtained using Planmeca ProMax 3D Mid instrument (Planmeca, Helsinki, Finland) and analyzed using Planmeca Romexis Viewer software. In the sagittal section, the vertical distance from the root apex to the upper projection of the mandibular canal was measured for each examined tooth. Results. The distal root of the first molar (4.88 mm) had the greatest average vertical distance of the root apex from the mandibular canal, and the distal root of the second molar had the smallest average vertical distance (2.76 mm). There was statistically significant difference between certain age groups in the values of individual roots, for the second molar mesially and distally (p < 0.05), while for the first molar mesially the value of p was at the limit of significance (p = 0.05). Conclusion. The results of this study showed that distal root of the mandibular second molar had the smallest vertical distance from mandibular canal, therefore an extra caution during a root canal treatment and careful planning of oral surgery in this region is recommended.
介绍。下颌管及其相关的神经血管束可能与下颌牙尖密切相关。为了避免在有创牙科手术中损伤和损害下牙槽神经,了解其定位是很重要的。本研究的目的是确定第二前磨牙、第一磨牙和第二磨牙的牙根尖在CBCT矢状面图像上近端和远端距离下颌管上突的平均垂直距离,并确定年龄和性别组之间是否存在统计学差异。材料和方法。这项研究是在巴尼亚卢卡大学医学院进行的,样本包括146张患者的CBCT图像。使用Planmeca ProMax 3D Mid仪器(Planmeca, Helsinki, Finland)获取CBCT图像,并使用Planmeca Romexis Viewer软件进行分析。矢状面测量每颗被检查牙齿从牙根尖到下颌管上突的垂直距离。结果。第一磨牙远端根尖距下颌管平均垂直距离最大(4.88 mm),第二磨牙远端根尖距下颌管平均垂直距离最小(2.76 mm)。第二磨牙近端和远端单根值在特定年龄组间差异有统计学意义(p < 0.05),而第一磨牙近端p值无统计学意义(p = 0.05)。结论。本研究结果显示,下颌第二磨牙远端根与下颌根管的垂直距离最小,因此在进行根管治疗时要格外小心,并建议仔细规划该区域的口腔手术。
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引用次数: 0
Infection prevention and control protocols in dentistry - Canadian guidelines 牙科感染预防和控制方案。加拿大指南
Pub Date : 1900-01-01 DOI: 10.2298/sgs2202072s
S. Stojičić, Vladimir Obrenović
Introduction. A risk of transmission of infectious diseases has always been an inherent part of dental practice. Easily communicable respiratory and blood borne diseases have prompted dental communities to establish, evaluate, update and monitor infection prevention and control (IPAC) protocols and strategies (guidelines). The aim of this paper was to present Canadian standard protocols of infection prevention and control in dental offices, and compare them with similar guidelines available to dentists in Serbia. Method. A detailed overview of the most current IPAC guidelines and protocols provided by the College of Dental Surgeons of British Columbia in Canada has been summarized. In addition, the effect of the most recent Covid-19 pandemic on the current infection prevention and control measures in dental offices and future perspectives has been reviewed. Conclusion. Implementing infection prevention and control guidelines is essential part of practicing dentistry. Regulatory authorities have responsibility to establish and provide dentist with the most current IPAC protocols while dentists need to adopt up-to-date procedures and appropriately and consistently use them in everyday practice.
介绍。传染病传播的风险一直是牙科实践的固有部分。容易传染的呼吸道和血液传播疾病促使牙科社区建立、评估、更新和监测感染预防和控制(IPAC)协议和战略(准则)。本文的目的是提出加拿大牙科诊所感染预防和控制的标准协议,并将其与塞尔维亚牙医可用的类似指南进行比较。方法。对加拿大不列颠哥伦比亚省牙科外科学院提供的最新IPAC指南和协议进行了详细概述。此外,还综述了最近一次Covid-19大流行对牙科诊所当前感染防控措施的影响以及未来的展望。结论。实施感染预防和控制指南是牙科执业的重要组成部分。监管机构有责任建立并向牙医提供最新的IPAC协议,而牙医需要采用最新的程序,并在日常实践中适当和一致地使用它们。
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引用次数: 0
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Serbian Dental Journal
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