首页 > 最新文献

Macromolecular bioscience最新文献

英文 中文
Biochemical Signal-Induced Supramolecular Hydrogelation for Structured Free-Standing Soft Material Formation. 生化信号诱导超分子水凝胶化,形成结构化的独立软材料。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-15 DOI: 10.1002/mabi.202400419
Dineshkumar Bharathidasan, Akshay Sunil Salvi, Suryasarathi Bose, Chandan Maity

Cells coordinate their activity and regulate biological processes in response to chemical signals. Mimicking natural processes, control over the formation of artificial supramolecular materials is of high interest for their application in biology and medicine. Supramolecular material that can form in response to chemical signals is important for the development of autonomously responsive materials. Herein, a supramolecular hydrogel system is reported enabling in situ generation of hydrogelators in response to a specific chemical signal. Using self-immolative chemistry, spatial control over the formation of supramolecular hydrogel material and structured free-standing hydrogel objects via providing H2O2 locally is demonstrated. In addition, a hybrid system is developed enabling in situ generation of the H2O2 by the action of an enzyme and glucose, providing an extra handle for the development of an intelligent soft material. This generic design should enable the use of various (chemical)stimuli that can be obtained via coupling different stimuli and various chemical and/or biological markers and appears a versatile approach for the design of smart artificial soft materials that can find application in theranostic purposes.

细胞根据化学信号协调其活动并调节生物过程。模仿自然过程,控制人造超分子材料的形成,对其在生物和医学领域的应用具有极大的兴趣。能根据化学信号形成的超分子材料对于开发自主响应材料非常重要。本文报告了一种超分子水凝胶系统,该系统可根据特定化学信号在原位生成水凝胶。利用自惰性化学,通过在局部提供 H2O2,展示了对超分子水凝胶材料和结构化独立水凝胶物体形成的空间控制。此外,还开发了一种混合系统,通过酶和葡萄糖的作用在原位生成 H2O2,为开发智能软材料提供了额外的把手。这种通用设计可以使用各种(化学)刺激,这些刺激可以通过将不同的刺激和各种化学和/或生物标记耦合在一起而获得。
{"title":"Biochemical Signal-Induced Supramolecular Hydrogelation for Structured Free-Standing Soft Material Formation.","authors":"Dineshkumar Bharathidasan, Akshay Sunil Salvi, Suryasarathi Bose, Chandan Maity","doi":"10.1002/mabi.202400419","DOIUrl":"https://doi.org/10.1002/mabi.202400419","url":null,"abstract":"<p><p>Cells coordinate their activity and regulate biological processes in response to chemical signals. Mimicking natural processes, control over the formation of artificial supramolecular materials is of high interest for their application in biology and medicine. Supramolecular material that can form in response to chemical signals is important for the development of autonomously responsive materials. Herein, a supramolecular hydrogel system is reported enabling in situ generation of hydrogelators in response to a specific chemical signal. Using self-immolative chemistry, spatial control over the formation of supramolecular hydrogel material and structured free-standing hydrogel objects via providing H<sub>2</sub>O<sub>2</sub> locally is demonstrated. In addition, a hybrid system is developed enabling in situ generation of the H<sub>2</sub>O<sub>2</sub> by the action of an enzyme and glucose, providing an extra handle for the development of an intelligent soft material. This generic design should enable the use of various (chemical)stimuli that can be obtained via coupling different stimuli and various chemical and/or biological markers and appears a versatile approach for the design of smart artificial soft materials that can find application in theranostic purposes.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400419"},"PeriodicalIF":4.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanically Stable and Biocompatible Polymer Brush Coated Dental Materials with Lubricious and Antifouling Properties 具有润滑和防污特性的机械稳定且生物兼容的聚合物刷涂牙科材料
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-15 DOI: 10.1002/mabi.202470026
Rong Mu, Ling Yang, Xinyue Wang, Binrui Yang, Jia Li, Aijun Wang, Guorui Zhang, Chufeng Sun, Yang Wu, Bo Yu, Bin Li

Front Cover: Polymer brushes are grafted to the substrate through surface-initiated atom transfer radical polymerization (SI-ATRP) to form a durable polymeric layer, which enhances surface lubrication, reduces bacterial adhesion, and improves biocompatibility and anti-inflammatory properties. The polymer brush coating shows potential application in dental materials. More details can be found in article 2400194 by Jia Li, Guorui Zhang, Bin Li, and co-workers.

封面:通过表面引发原子转移自由基聚合(SI-ATPR)将聚合物刷接枝到基底上,形成耐久的聚合物层,从而增强表面润滑性,减少细菌粘附,改善生物相容性和消炎特性。这种聚合物刷涂层具有应用于牙科材料的潜力。更多详情,请参阅李佳、张国瑞、李斌及合作者的文章 2400194。
{"title":"Mechanically Stable and Biocompatible Polymer Brush Coated Dental Materials with Lubricious and Antifouling Properties","authors":"Rong Mu,&nbsp;Ling Yang,&nbsp;Xinyue Wang,&nbsp;Binrui Yang,&nbsp;Jia Li,&nbsp;Aijun Wang,&nbsp;Guorui Zhang,&nbsp;Chufeng Sun,&nbsp;Yang Wu,&nbsp;Bo Yu,&nbsp;Bin Li","doi":"10.1002/mabi.202470026","DOIUrl":"https://doi.org/10.1002/mabi.202470026","url":null,"abstract":"<p><b>Front Cover</b>: Polymer brushes are grafted to the substrate through surface-initiated atom transfer radical polymerization (SI-ATRP) to form a durable polymeric layer, which enhances surface lubrication, reduces bacterial adhesion, and improves biocompatibility and anti-inflammatory properties. The polymer brush coating shows potential application in dental materials. More details can be found in article 2400194 by Jia Li, Guorui Zhang, Bin Li, and co-workers.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 11","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Situ Forming Injectable Gelatin-Based Antibacterial Bioadhesives for Preventing Postoperative Leakage and Abdominal Adhesions. 用于防止术后渗漏和腹部粘连的原位成型明胶基抗菌生物粘合剂。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-08 DOI: 10.1002/mabi.202400372
Shibo Hu, Wanglin Duan, Xianzhen Jin, Chaowei Li, Bin Zhu, Yurong Chen, Ye Zhu, Peihai Cao, Zepeng Dong, Luyao Feng, Junhui Yu, Xuejun Sun, Zeshaan Yahaya Haji Mahmood, Yazhong Bu, Baoji Du, Jianbao Zheng

Postoperative anastomotic leakage (AL) and abdominal adhesions are two major complications after intestinal surgery, with an incidence of 2-25% for AL and 93% for adhesion. Until now, there is no method addressing AL and abdominal adhesions simultaneously. In this work, Bi-PEG-succinimidyl succinate (PEG-NHS), amino-gelatin (Agel) is combined with cefoperazone-sulbactam (SCF) to prepare a multifunctional bioadhesive (SCF/SEAgel) for the postoperative leakage and adhesion prevention. SCF/SEAgel possesses a great sealing capability for tissue, with a bursting pressure of 54 kPa. The loaded SCF endows the systems with good antibacterial properties. The in vivo antiadhesion experiments show that SCF/SEAgel possesses better anti-adhesion properties than the commercially used sodium hyaluronate gel. In the cecum leakage model, the SCF/SEAgel effectively seals the leakage with a survival rate of 100%, superior to commercial products (Sainaoning). Meanwhile, it significantly reduces tissue adhesion. Finally, the laparoscopic surgery with dogs shows that the SCF/SEAgel can be injected through minimally invasive surgery, demonstrating its ease of use. Combined with its great biocompatibility, SCF/SEAgel is very promising in intestinal surgery.

术后吻合口漏(AL)和腹腔粘连是肠道手术后的两大并发症,AL 发生率为 2-25%,粘连发生率为 93%。迄今为止,还没有一种方法能同时解决 AL 和腹腔粘连问题。在这项研究中,双 PEG-琥珀酰亚胺丁二酸酯(PEG-NHS)、氨基明胶(Agel)与头孢哌酮-舒巴坦(SCF)结合制备了一种多功能生物粘合剂(SCF/SEAgel),用于术后渗漏和粘连的预防。SCF/SEAgel 对组织具有很强的密封能力,其破裂压力为 54 kPa。负载的 SCF 使该系统具有良好的抗菌性能。体内抗粘连实验表明,SCF/SEA凝胶的抗粘连性能优于市售的透明质酸钠凝胶。在盲肠渗漏模型中,SCF/SEAgel 能有效封堵渗漏,存活率达 100%,优于市售产品(赛宁)。同时,它还能明显减少组织粘连。最后,用狗进行的腹腔镜手术表明,SCF/SEAgel 可以通过微创手术进行注射,证明了它的易用性。SCF/SEAgel 具有良好的生物相容性,因此在肠道手术中大有可为。
{"title":"In Situ Forming Injectable Gelatin-Based Antibacterial Bioadhesives for Preventing Postoperative Leakage and Abdominal Adhesions.","authors":"Shibo Hu, Wanglin Duan, Xianzhen Jin, Chaowei Li, Bin Zhu, Yurong Chen, Ye Zhu, Peihai Cao, Zepeng Dong, Luyao Feng, Junhui Yu, Xuejun Sun, Zeshaan Yahaya Haji Mahmood, Yazhong Bu, Baoji Du, Jianbao Zheng","doi":"10.1002/mabi.202400372","DOIUrl":"https://doi.org/10.1002/mabi.202400372","url":null,"abstract":"<p><p>Postoperative anastomotic leakage (AL) and abdominal adhesions are two major complications after intestinal surgery, with an incidence of 2-25% for AL and 93% for adhesion. Until now, there is no method addressing AL and abdominal adhesions simultaneously. In this work, Bi-PEG-succinimidyl succinate (PEG-NHS), amino-gelatin (Agel) is combined with cefoperazone-sulbactam (SCF) to prepare a multifunctional bioadhesive (SCF/SEAgel) for the postoperative leakage and adhesion prevention. SCF/SEAgel possesses a great sealing capability for tissue, with a bursting pressure of 54 kPa. The loaded SCF endows the systems with good antibacterial properties. The in vivo antiadhesion experiments show that SCF/SEAgel possesses better anti-adhesion properties than the commercially used sodium hyaluronate gel. In the cecum leakage model, the SCF/SEAgel effectively seals the leakage with a survival rate of 100%, superior to commercial products (Sainaoning). Meanwhile, it significantly reduces tissue adhesion. Finally, the laparoscopic surgery with dogs shows that the SCF/SEAgel can be injected through minimally invasive surgery, demonstrating its ease of use. Combined with its great biocompatibility, SCF/SEAgel is very promising in intestinal surgery.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400372"},"PeriodicalIF":4.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyamino Acid Based Zwitterionic Coating can Inhibit Coagulation and Inflammation Through Anti-Fouling and Restoring Microenvironment. 聚氨基酸型聚合离子涂层可通过防污和恢复微环境来抑制凝血和炎症。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-08 DOI: 10.1002/mabi.202400336
Zehong Xiang, Honghong Chen, Feng Wu, Haobo Pan

Protein adhesion and thrombosis formation caused by limited surface properties pose great challenges to biomedical implants. Although various hydrophilic coating or drug release coatings are reported, the single coating cannot cope with cases under the condition of complex physiological environment, which causes the coating effect is limited. In this study, a polyamino acid-derived zwitterionic coating is constructed to eliminate reactive oxygen species (ROS) in the microenvironment. It is demonstrated that the coating has excellent hydrophilicity, stability, and lubricity, and can obviously prevent protein adhesion. At the same time, the coating can eliminate hydrogen peroxide and maintain the stability of the microenvironment. The in vivo and in vitro experiments show that the coating has good biocompatibility, and inhibits thrombus. Amino acid zwitterion coating prevents protein deposition, alleviates the inflammatory process, inhibit of thrombosis, reduces the risk of implantable medical devices, and prolongs their service time. Hence, the work paves a new way to develop amino acid based zwitterionic polymer coating that can reduce the implant complications.

由于表面性能有限而导致的蛋白质粘附和血栓形成给生物医学植入物带来了巨大挑战。虽然目前已有多种亲水涂层或药物释放涂层的报道,但单一涂层无法应对复杂生理环境条件下的病例,导致涂层效果有限。本研究构建了一种源于聚氨基酸的齐聚物涂层,以消除微环境中的活性氧(ROS)。研究表明,该涂层具有良好的亲水性、稳定性和润滑性,能明显防止蛋白质粘附。同时,涂层还能消除过氧化氢,保持微环境的稳定性。体内和体外实验表明,涂层具有良好的生物相容性,并能抑制血栓形成。氨基酸齐聚物涂层可防止蛋白质沉积,缓解炎症过程,抑制血栓形成,降低植入式医疗器械的风险,延长其使用寿命。因此,这项研究为开发能减少植入并发症的氨基酸型齐聚物涂层铺平了新的道路。
{"title":"Polyamino Acid Based Zwitterionic Coating can Inhibit Coagulation and Inflammation Through Anti-Fouling and Restoring Microenvironment.","authors":"Zehong Xiang, Honghong Chen, Feng Wu, Haobo Pan","doi":"10.1002/mabi.202400336","DOIUrl":"https://doi.org/10.1002/mabi.202400336","url":null,"abstract":"<p><p>Protein adhesion and thrombosis formation caused by limited surface properties pose great challenges to biomedical implants. Although various hydrophilic coating or drug release coatings are reported, the single coating cannot cope with cases under the condition of complex physiological environment, which causes the coating effect is limited. In this study, a polyamino acid-derived zwitterionic coating is constructed to eliminate reactive oxygen species (ROS) in the microenvironment. It is demonstrated that the coating has excellent hydrophilicity, stability, and lubricity, and can obviously prevent protein adhesion. At the same time, the coating can eliminate hydrogen peroxide and maintain the stability of the microenvironment. The in vivo and in vitro experiments show that the coating has good biocompatibility, and inhibits thrombus. Amino acid zwitterion coating prevents protein deposition, alleviates the inflammatory process, inhibit of thrombosis, reduces the risk of implantable medical devices, and prolongs their service time. Hence, the work paves a new way to develop amino acid based zwitterionic polymer coating that can reduce the implant complications.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400336"},"PeriodicalIF":4.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface Coating of ZIF-8 Nanoparticles with Polyacrylic Acid: A Facile Approach to Enhance Chemical Stability for Biomedical Applications. 用聚丙烯酸对 ZIF-8 纳米粒子进行表面涂层:增强生物医学应用中化学稳定性的简便方法。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-03 DOI: 10.1002/mabi.202400382
Setsuko Yamane, Abdul Hadi Bin Yusri, Po-Yu Chen, André J van der Vlies, Amira Ben Mabrouk, Isabelle Fetzer, Urara Hasegawa

Nanoparticles of zeolitic imidazole framework-8 (ZIF-8 NPs), which are the subclass of metal-organic frameworks consisting of Zn ion and 2-methylimidazole, have been identified as promising drug carriers since their large microporous structure is suited for encapsulating hydrophobic drug molecules. However, one of the limitations of ZIF-8 NPs is their low stability in physiological solutions, especially in the presence of water and phosphate anions. These molecules can interact with the coordinatively unsaturated Zn sites at the external surface to induce the degradation of ZIF-8 NPs. In this study, herein a facile approach is reported to enhance the chemical stability of ZIF-8 NPs by surface coating with polyacrylic acid (PAA). The PAA-coated ZIF-8 (PAA-ZIF-8) NPs are prepared by mixing ZIF-8 NPs and PAA in water. PAA coating inhibits the degradation of ZIF-8 NPs in water as well as phosphate-buffered saline over 6 days, which seems to be due to the coordination of carboxyl groups of PAA to the reactive Zn sites. Furthermore, the PAA-ZIF-8 NPs loaded with the anticancer drug doxorubicin (Dox) show cytotoxicity in human colon cancer cells. These results clearly show the feasibility of the PAA coating approach to improve the chemical stability of ZIF-8 NPs without impairing their drug delivery capability.

沸石咪唑框架-8(ZIF-8 NPs)纳米粒子是由锌离子和 2-甲基咪唑组成的金属有机框架的亚类,由于其大微孔结构适合封装疏水性药物分子,因此被认为是很有前途的药物载体。然而,ZIF-8 NPs 的局限性之一是其在生理溶液中的稳定性较低,尤其是在有水和磷酸盐阴离子存在的情况下。这些分子会与外表面配位不饱和的锌位点相互作用,导致 ZIF-8 NPs 降解。本研究采用一种简便的方法,通过表面涂覆聚丙烯酸(PAA)来增强 ZIF-8 NPs 的化学稳定性。PAA 涂层 ZIF-8 (PAA-ZIF-8) NPs 由 ZIF-8 NPs 和 PAA 在水中混合制备而成。PAA 涂层可抑制 ZIF-8 NPs 在水中和磷酸盐缓冲盐水中 6 天的降解,这似乎是由于 PAA 的羧基与反应性 Zn 位点配位所致。此外,负载抗癌药物多柔比星(Dox)的 PAA-ZIF-8 NPs 对人类结肠癌细胞具有细胞毒性。这些结果清楚地表明了 PAA 涂层方法在不影响 ZIF-8 NPs 药物输送能力的前提下提高其化学稳定性的可行性。
{"title":"Surface Coating of ZIF-8 Nanoparticles with Polyacrylic Acid: A Facile Approach to Enhance Chemical Stability for Biomedical Applications.","authors":"Setsuko Yamane, Abdul Hadi Bin Yusri, Po-Yu Chen, André J van der Vlies, Amira Ben Mabrouk, Isabelle Fetzer, Urara Hasegawa","doi":"10.1002/mabi.202400382","DOIUrl":"https://doi.org/10.1002/mabi.202400382","url":null,"abstract":"<p><p>Nanoparticles of zeolitic imidazole framework-8 (ZIF-8 NPs), which are the subclass of metal-organic frameworks consisting of Zn ion and 2-methylimidazole, have been identified as promising drug carriers since their large microporous structure is suited for encapsulating hydrophobic drug molecules. However, one of the limitations of ZIF-8 NPs is their low stability in physiological solutions, especially in the presence of water and phosphate anions. These molecules can interact with the coordinatively unsaturated Zn sites at the external surface to induce the degradation of ZIF-8 NPs. In this study, herein a facile approach is reported to enhance the chemical stability of ZIF-8 NPs by surface coating with polyacrylic acid (PAA). The PAA-coated ZIF-8 (PAA-ZIF-8) NPs are prepared by mixing ZIF-8 NPs and PAA in water. PAA coating inhibits the degradation of ZIF-8 NPs in water as well as phosphate-buffered saline over 6 days, which seems to be due to the coordination of carboxyl groups of PAA to the reactive Zn sites. Furthermore, the PAA-ZIF-8 NPs loaded with the anticancer drug doxorubicin (Dox) show cytotoxicity in human colon cancer cells. These results clearly show the feasibility of the PAA coating approach to improve the chemical stability of ZIF-8 NPs without impairing their drug delivery capability.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400382"},"PeriodicalIF":4.4,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Application of Biomaterial-Based Spinal Cord Tissue Engineering. 基于生物材料的脊髓组织工程的应用。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-29 DOI: 10.1002/mabi.202400444
Liang Ma, Zhen Zhang, Yulei Mu, Bangheng Liu, Huiqun Zhou, Dong-An Wang

Advancements in biomaterial-based spinal cord tissue engineering technology have profoundly influenced regenerative medicine, providing innovative solutions for both spinal cord organoid development and engineered spinal cord injury (SCI) repair. In spinal cord organoids, biomaterials offer a supportive microenvironment that mimics the natural extracellular matrix, facilitating cell differentiation and organization and advancing the understanding of spinal cord development and pathophysiology. Furthermore, biomaterials are essential in constructing engineered spinal cords for SCI repair. The incorporation of biomaterials with growth factors, fabrication of ordered scaffold structures, and artificial spinal cord assemblies are critical insights for SCI to ensure structural integrity, enhance cell viability, and promote neural regeneration in transplantation. In summary, this review summarizes the contribution of biomaterials to the spinal cord organoids progression and discusses strategies for biomaterial-based spinal cord engineering in SCI therapy. These achievements underscore the transformative potential of biomaterials to improve treatment options for SCI and accelerate future clinical applications.

基于生物材料的脊髓组织工程技术的进步对再生医学产生了深远的影响,为脊髓类器官的发育和脊髓损伤(SCI)的工程修复提供了创新的解决方案。在脊髓类器官中,生物材料提供了模拟天然细胞外基质的支持性微环境,促进了细胞的分化和组织,增进了对脊髓发育和病理生理学的了解。此外,生物材料对于构建用于 SCI 修复的工程脊髓至关重要。生物材料与生长因子的结合、有序支架结构的制造以及人工脊髓组装是治疗 SCI 的关键见解,可确保结构完整性、提高细胞活力并促进移植中的神经再生。总之,本综述总结了生物材料对脊髓器官组织进展的贡献,并讨论了基于生物材料的脊髓工程在 SCI 治疗中的应用策略。这些成就强调了生物材料在改善 SCI 治疗方案和加速未来临床应用方面的变革潜力。
{"title":"The Application of Biomaterial-Based Spinal Cord Tissue Engineering.","authors":"Liang Ma, Zhen Zhang, Yulei Mu, Bangheng Liu, Huiqun Zhou, Dong-An Wang","doi":"10.1002/mabi.202400444","DOIUrl":"https://doi.org/10.1002/mabi.202400444","url":null,"abstract":"<p><p>Advancements in biomaterial-based spinal cord tissue engineering technology have profoundly influenced regenerative medicine, providing innovative solutions for both spinal cord organoid development and engineered spinal cord injury (SCI) repair. In spinal cord organoids, biomaterials offer a supportive microenvironment that mimics the natural extracellular matrix, facilitating cell differentiation and organization and advancing the understanding of spinal cord development and pathophysiology. Furthermore, biomaterials are essential in constructing engineered spinal cords for SCI repair. The incorporation of biomaterials with growth factors, fabrication of ordered scaffold structures, and artificial spinal cord assemblies are critical insights for SCI to ensure structural integrity, enhance cell viability, and promote neural regeneration in transplantation. In summary, this review summarizes the contribution of biomaterials to the spinal cord organoids progression and discusses strategies for biomaterial-based spinal cord engineering in SCI therapy. These achievements underscore the transformative potential of biomaterials to improve treatment options for SCI and accelerate future clinical applications.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400444"},"PeriodicalIF":4.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biocompatible Zn-Phthalocyanine/Gelatin Nanofiber Membrane for Antibacterial Therapy. 用于抗菌治疗的生物相容性锌-酞菁/明胶纳米纤维膜
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-29 DOI: 10.1002/mabi.202400334
Romina Clementi, Maria Angela Vargas, Mariana Cid, Nancy Salvatierra, Romina Comín, Tomas Tempesti

In this study, the fabrication and characterization of Zn-phthalocyanine/gelatin nanofibrous membranes is reported using the electrospinning technique. The membranes exhibit a homogeneous distribution of Zn-phthalocyanine within the gelatin matrix, maintaining the structural integrity and photosensitizing properties of the phthalocyanine. Scanning electron microscopy revealed that the electrospun fibers possess diameters ranging results as 100-300, 200-700, and 300-800 nm for Gel, ZnPc/Gel 1, and ZnPc/Gel 2, respectively. The addition of ZnPc does not decrease the hydrophilicity of the Gel membrane. The nanofibrous membranes showed good cytocompatibility, as indicated by the high viability of Vero cells exposed to membrane extracts. Furthermore, these composites supported cell adhesion and proliferation on their surfaces. The two Zn-phthalocyanine/gelatin nanofiber formulations exhibited significant antimicrobial activity toward Escherichia Coli (E. Coli) and Staphylococcus Aureus (S. Aureus) under visible light illumination, achieving reductions of 3.4 log10 and 3.6 log10 CFU mL-1 for E. coli, and 3.9 log10 and 4.1 log10 CFU mL-1 for S. aureus. These results demonstrate the potential of Zn-phthalocyanine/gelatin nanofibrous membranes as effective agents in antibacterial photodynamic therapy, providing a promising solution to control bacterial infections and antibiotic resistance.

本研究采用电纺丝技术制备了酞菁锌/明胶纳米纤维膜,并对其进行了表征。这种膜在明胶基质中呈现出酞菁锌的均匀分布,保持了酞菁的结构完整性和光敏特性。扫描电子显微镜显示,凝胶、ZnPc/凝胶 1 和 ZnPc/Gel 2 电纺纤维的直径分别为 100-300、200-700 和 300-800 纳米。添加 ZnPc 不会降低凝胶膜的亲水性。纳米纤维膜具有良好的细胞相容性,暴露在膜提取物中的 Vero 细胞具有很高的存活率。此外,这些复合材料还支持细胞在其表面粘附和增殖。在可见光照射下,两种锌酞菁/明胶纳米纤维配方对大肠杆菌(E. Coli)和金黄色葡萄球菌(S. Aureus)具有显著的抗菌活性,大肠杆菌的抗菌活性分别降低了 3.4 log10 和 3.6 log10 CFU mL-1,金黄色葡萄球菌的抗菌活性分别降低了 3.9 log10 和 4.1 log10 CFU mL-1。这些结果证明了掺酞菁锌/明胶纳米纤维膜作为抗菌光动力疗法有效制剂的潜力,为控制细菌感染和抗生素耐药性提供了一种前景广阔的解决方案。
{"title":"Biocompatible Zn-Phthalocyanine/Gelatin Nanofiber Membrane for Antibacterial Therapy.","authors":"Romina Clementi, Maria Angela Vargas, Mariana Cid, Nancy Salvatierra, Romina Comín, Tomas Tempesti","doi":"10.1002/mabi.202400334","DOIUrl":"https://doi.org/10.1002/mabi.202400334","url":null,"abstract":"<p><p>In this study, the fabrication and characterization of Zn-phthalocyanine/gelatin nanofibrous membranes is reported using the electrospinning technique. The membranes exhibit a homogeneous distribution of Zn-phthalocyanine within the gelatin matrix, maintaining the structural integrity and photosensitizing properties of the phthalocyanine. Scanning electron microscopy revealed that the electrospun fibers possess diameters ranging results as 100-300, 200-700, and 300-800 nm for Gel, ZnPc/Gel 1, and ZnPc/Gel 2, respectively. The addition of ZnPc does not decrease the hydrophilicity of the Gel membrane. The nanofibrous membranes showed good cytocompatibility, as indicated by the high viability of Vero cells exposed to membrane extracts. Furthermore, these composites supported cell adhesion and proliferation on their surfaces. The two Zn-phthalocyanine/gelatin nanofiber formulations exhibited significant antimicrobial activity toward Escherichia Coli (E. Coli) and Staphylococcus Aureus (S. Aureus) under visible light illumination, achieving reductions of 3.4 log<sub>10</sub> and 3.6 log<sub>10</sub> CFU mL<sup>-1</sup> for E. coli, and 3.9 log<sub>10</sub> and 4.1 log<sub>10</sub> CFU mL<sup>-1</sup> for S. aureus. These results demonstrate the potential of Zn-phthalocyanine/gelatin nanofibrous membranes as effective agents in antibacterial photodynamic therapy, providing a promising solution to control bacterial infections and antibiotic resistance.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400334"},"PeriodicalIF":4.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Applications of Diels-Alder Chemistry in Biomaterials and Drug Delivery. Diels-Alder 化学在生物材料和药物输送中的应用。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-26 DOI: 10.1002/mabi.202400274
Tyus J Yeingst, Angelica M Helton, Daniel J Hayes

Recent studies, leveraging click chemistry reactions, have significantly advanced the fields of biomaterials and drug delivery. Of these click reactions, the Diels-Alder cycloaddition is exceptionally valuable for synthetic organic chemistry and biomaterial design, as it occurs under mild reaction conditions and can undergo a retrograde reaction, under physiologically relevant conditions, to yield the initial reactants. In this review, potential applications of the Diels-Alder reaction are explored within the nexus of biomaterials and drug delivery. This includes an emphasis on key platforms such as polymers, nanoparticles, and hydrogels which utilize Diels-Alder for drug delivery, functionalized surfaces, bioconjugation, and other diverse applications. Specifically, this review will focus on the use of Diels-Alder biomaterials in applications of tissue engineering and cancer therapies, while providing a discussion of the advantages, platforms, and applications of Diels-Alder click chemistry.

最近的研究利用点击化学反应大大推动了生物材料和药物输送领域的发展。在这些点击反应中,Diels-Alder 环加成反应对合成有机化学和生物材料设计具有特别重要的价值,因为它发生在温和的反应条件下,并能在生理相关条件下发生逆反应,生成初始反应物。本综述探讨了 Diels-Alder 反应在生物材料和药物输送领域的潜在应用。其中包括聚合物、纳米颗粒和水凝胶等关键平台,这些平台利用 Diels-Alder 进行药物输送、功能化表面、生物共轭和其他各种应用。具体而言,本综述将重点关注 Diels-Alder 生物材料在组织工程和癌症疗法中的应用,同时讨论 Diels-Alder 点击化学的优势、平台和应用。
{"title":"Applications of Diels-Alder Chemistry in Biomaterials and Drug Delivery.","authors":"Tyus J Yeingst, Angelica M Helton, Daniel J Hayes","doi":"10.1002/mabi.202400274","DOIUrl":"https://doi.org/10.1002/mabi.202400274","url":null,"abstract":"<p><p>Recent studies, leveraging click chemistry reactions, have significantly advanced the fields of biomaterials and drug delivery. Of these click reactions, the Diels-Alder cycloaddition is exceptionally valuable for synthetic organic chemistry and biomaterial design, as it occurs under mild reaction conditions and can undergo a retrograde reaction, under physiologically relevant conditions, to yield the initial reactants. In this review, potential applications of the Diels-Alder reaction are explored within the nexus of biomaterials and drug delivery. This includes an emphasis on key platforms such as polymers, nanoparticles, and hydrogels which utilize Diels-Alder for drug delivery, functionalized surfaces, bioconjugation, and other diverse applications. Specifically, this review will focus on the use of Diels-Alder biomaterials in applications of tissue engineering and cancer therapies, while providing a discussion of the advantages, platforms, and applications of Diels-Alder click chemistry.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400274"},"PeriodicalIF":4.4,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Manufacturing Radially Aligned PCL Nanofibers Reinforced With Sulfated Levan and Evaluation of its Biological Activity for Healing Tympanic Membrane Perforations. 用硫酸化莱凡制造径向排列的 PCL 纳米纤维并评估其愈合鼓膜穿孔的生物活性。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-26 DOI: 10.1002/mabi.202400291
Busra Akgul, Cansu Gulcan, Selay Tornaci, Merve Erginer, Ebru Toksoy Oner, Emrah Sefik Abamor, Serap Acar, Adil M Allahverdiyev

The main objective of this study is to construct radially aligned PCL nanofibers reinforced with levan polymer and investigate their in vitro biological activities thoroughly. First Halomonas levan (HL) polysaccharide is hydrolyzed (hHL) and subjected to sulfation to attain Sulfated hydrolyzed Halomonas levan (ShHL)-based material indicating heparin mimetic properties. Then, optimization studies are carried out to produce coaxially generated radially aligned Poly(caprolactone) (PCL) -ShHL nanofibers via electrospinning. The obtained nanofibers are characterized with Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray (FESEM-EDX) analysis, and mechanical, contact angle measurement, biodegradability, and swelling tests as well. Afterward, cytotoxicity of artificial tympanic membranes is analyzed by MTT (3-(4,5-Dimethylthiazol-2-yl) -2,5 Diphenyltetrazolium Bromide) test, and their impacts on cell proliferation, cellular adhesion, wound healing processes are explored. Furthermore, an additional FESEM imaging is performed to manifest the interactions between fibroblasts and nanofibers. According to analytical measurements it is detected that PCL-ShHL nanofibers i) are smaller in fiber diameter, ii) are more biodegradable, iii) are more hydrophilic, and iv) demonstrated superior mechanical properties compared to PCL nanofibers. Moreover, it is also deciphered that PCL-ShHL nanofibers strongly elevated cellular adhesion, proliferation, and in vitro wound healing features compared to PCL nanofibers. According to obtained results it is assumed that newly synthetized levan and PCL mediated nanofibers are very encouraging for healing tympanic membrane perforations.

本研究的主要目的是用莱万聚合物构建径向排列的 PCL 纳米纤维,并深入研究其体外生物活性。首先水解 Halomonas levan(HL)多糖(hHL)并进行硫酸化处理,以获得硫酸化水解 Halomonas levan(ShHL)基材料,该材料具有肝素模拟特性。然后进行优化研究,通过电纺丝生产出同轴径向排列的聚己内酯(PCL)-ShHL 纳米纤维。通过傅立叶变换红外光谱(FTIR)和场发射扫描电子显微镜与能量色散 X 射线(FESEM-EDX)分析,以及力学、接触角测量、生物降解性和溶胀测试,对获得的纳米纤维进行了表征。随后,通过 MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑)测试分析了人工鼓膜的细胞毒性,并探讨了它们对细胞增殖、细胞粘附和伤口愈合过程的影响。此外,还进行了额外的 FESEM 成像,以显示成纤维细胞和纳米纤维之间的相互作用。分析测量结果表明,与 PCL 纳米纤维相比,PCL-ShHL 纳米纤维 i) 纤维直径更小;ii) 生物可降解性更好;iii) 亲水性更强;iv) 机械性能更优越。此外,与 PCL 纳米纤维相比,PCL-ShHL 纳米纤维还能显著提高细胞粘附性、增殖性和体外伤口愈合功能。根据所获得的结果,可以认为新合成的 Levan 和 PCL 介导的纳米纤维对鼓膜穿孔的愈合非常有帮助。
{"title":"Manufacturing Radially Aligned PCL Nanofibers Reinforced With Sulfated Levan and Evaluation of its Biological Activity for Healing Tympanic Membrane Perforations.","authors":"Busra Akgul, Cansu Gulcan, Selay Tornaci, Merve Erginer, Ebru Toksoy Oner, Emrah Sefik Abamor, Serap Acar, Adil M Allahverdiyev","doi":"10.1002/mabi.202400291","DOIUrl":"https://doi.org/10.1002/mabi.202400291","url":null,"abstract":"<p><p>The main objective of this study is to construct radially aligned PCL nanofibers reinforced with levan polymer and investigate their in vitro biological activities thoroughly. First Halomonas levan (HL) polysaccharide is hydrolyzed (hHL) and subjected to sulfation to attain Sulfated hydrolyzed Halomonas levan (ShHL)-based material indicating heparin mimetic properties. Then, optimization studies are carried out to produce coaxially generated radially aligned Poly(caprolactone) (PCL) -ShHL nanofibers via electrospinning. The obtained nanofibers are characterized with Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray (FESEM-EDX) analysis, and mechanical, contact angle measurement, biodegradability, and swelling tests as well. Afterward, cytotoxicity of artificial tympanic membranes is analyzed by MTT (3-(4,5-Dimethylthiazol-2-yl) -2,5 Diphenyltetrazolium Bromide) test, and their impacts on cell proliferation, cellular adhesion, wound healing processes are explored. Furthermore, an additional FESEM imaging is performed to manifest the interactions between fibroblasts and nanofibers. According to analytical measurements it is detected that PCL-ShHL nanofibers i) are smaller in fiber diameter, ii) are more biodegradable, iii) are more hydrophilic, and iv) demonstrated superior mechanical properties compared to PCL nanofibers. Moreover, it is also deciphered that PCL-ShHL nanofibers strongly elevated cellular adhesion, proliferation, and in vitro wound healing features compared to PCL nanofibers. According to obtained results it is assumed that newly synthetized levan and PCL mediated nanofibers are very encouraging for healing tympanic membrane perforations.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400291"},"PeriodicalIF":4.4,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants. 探索不同脂肪细胞模型的形态和分子特性:单层、球形、结冷胶水凝胶和外植体
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-25 DOI: 10.1002/mabi.202400320
Franziska B Albrecht, Ann-Kathrin Schick, Annemarie Klatt, Freia F Schmidt, Svenja Nellinger, Petra J Kluger

White adipose tissue (WAT) plays a crucial role in energy homeostasis and secretes numerous adipokines with far-reaching effects. WAT is linked to diseases such as diabetes, cardiovascular disease, and cancer. There is a high demand for suitable in vitro models to study diseases and tissue metabolism. Most of these models are covered by 2D-monolayer cultures. This study aims to evaluate the performance of different WAT models to better derive potential applications. The stability of adipocyte characteristics in spheroids and two 3D gellan gum hydrogels with ex situ lobules and 2D-monolayer culture is analyzed. First, the differentiation to achieve adipocyte-like characteristics is determined. Second, to evaluate the maintenance of differentiated ASC-based models, an adipocyte-based model, and explants over 3 weeks, viability, intracellular lipid content, perilipin A expression, adipokine, and gene expression are analyzed. Several advantages are supported using each of the models. Including, but not limited to, the strong differentiation in 2D-monolayers, the self-assembling within spheroids, the long-term stability of the stem cell-containing hydrogels, and the mature phenotype within adipocyte-containing hydrogels and the lobules. This study highlights the advantages of 3D models due to their more in vivo-like behavior and provides an overview of the different adipose cell models.

白色脂肪组织(WAT)在能量平衡中起着至关重要的作用,并能分泌多种具有深远影响的脂肪因子。白脂肪组织与糖尿病、心血管疾病和癌症等疾病有关。研究疾病和组织代谢需要大量合适的体外模型。这些模型大多采用二维单层培养。本研究旨在评估不同 WAT 模型的性能,以更好地推导其潜在应用。研究分析了球形和两种三维结冷胶水凝胶与原位小叶和二维单层培养中脂肪细胞特性的稳定性。首先,确定分化以获得类似脂肪细胞的特征。其次,为了评估已分化的基于 ASC 的模型、基于脂肪细胞的模型和外植体在 3 周内的维持情况,对活力、细胞内脂质含量、过脂素 A 表达、脂肪因子和基因表达进行了分析。每种模型都有若干优点。包括但不限于二维单层中的强分化、球体内的自组装、含干细胞水凝胶的长期稳定性以及含脂肪细胞水凝胶和小叶内的成熟表型。这项研究强调了三维模型的优势,因为它们更像活体行为,并概述了不同的脂肪细胞模型。
{"title":"Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants.","authors":"Franziska B Albrecht, Ann-Kathrin Schick, Annemarie Klatt, Freia F Schmidt, Svenja Nellinger, Petra J Kluger","doi":"10.1002/mabi.202400320","DOIUrl":"https://doi.org/10.1002/mabi.202400320","url":null,"abstract":"<p><p>White adipose tissue (WAT) plays a crucial role in energy homeostasis and secretes numerous adipokines with far-reaching effects. WAT is linked to diseases such as diabetes, cardiovascular disease, and cancer. There is a high demand for suitable in vitro models to study diseases and tissue metabolism. Most of these models are covered by 2D-monolayer cultures. This study aims to evaluate the performance of different WAT models to better derive potential applications. The stability of adipocyte characteristics in spheroids and two 3D gellan gum hydrogels with ex situ lobules and 2D-monolayer culture is analyzed. First, the differentiation to achieve adipocyte-like characteristics is determined. Second, to evaluate the maintenance of differentiated ASC-based models, an adipocyte-based model, and explants over 3 weeks, viability, intracellular lipid content, perilipin A expression, adipokine, and gene expression are analyzed. Several advantages are supported using each of the models. Including, but not limited to, the strong differentiation in 2D-monolayers, the self-assembling within spheroids, the long-term stability of the stem cell-containing hydrogels, and the mature phenotype within adipocyte-containing hydrogels and the lobules. This study highlights the advantages of 3D models due to their more in vivo-like behavior and provides an overview of the different adipose cell models.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400320"},"PeriodicalIF":4.4,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Macromolecular bioscience
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1