首页 > 最新文献

Macromolecular bioscience最新文献

英文 中文
Bioengineered Silk Fibroin Hydrogel Reinforced with Collagen-Like Protein Chimeras for Improved Wound Healing. 用胶原蛋白类嵌合体增强的生物工程蚕丝纤维素水凝胶可改善伤口愈合。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-18 DOI: 10.1002/mabi.202400346
Thiagarajan Hemalatha, Mayilvahanan Aarthy, Ashokraj Sundarapandiyan, Niraikulam Ayyadurai

The study investigates the potentials of the rapid crosslinking hydrogel concoction comprising of natural silk fibroin (SF) and recombinant tailorable collagen-like protein with binding domains for wound repair. The formation of dityrosine crosslinks between the tyrosine moieties augments the formation of stable hydrogels, in the presence of the cytocompatible photo-initiator riboflavin and visible light. This uniquely engineered PASCH (Photo-activated silk fibroin and tailor-made collagen-like protein hydrogel) confers the key advantage of improved biological properties over the control hydrogels comprising only of SF. The physico-chemical characterization of the hydrogels with respect to crosslinking, modulus, and thermal stability delineates the ascendancy of PASCH 7:3 over other combinations. Furthermore, the hybrid protein hydrogel proves to be a favorable cellular matrix as it enhances cell adhesion, elongation, growth, and proliferation in vitro. Time-lapse microscopy studies reveal an enhanced wound closure in human endothelial cell monolayer (EA.hy926), while the gene expression studies portray the dynamic interplay of cytokines and growth factors in the wound milieu facilitating the repair and regeneration of cells, sculpted by the proteins. The results demonstrate the improved physical and biological properties of fabricated PASCH, depicting their synergism, and implying their competency for use in tissue engineering applications.

这项研究探讨了由天然蚕丝纤维素(SF)和具有结合域的重组可定制胶原蛋白样蛋白组成的快速交联水凝胶混合物在伤口修复方面的潜力。在细胞相容性光引发剂核黄素和可见光的作用下,酪氨酸分子之间形成的双酪氨酸交联可促进稳定水凝胶的形成。这种独特设计的 PASCH(光激活蚕丝纤维素和定制胶原蛋白水凝胶)与仅由蚕丝纤维素组成的对照水凝胶相比,具有改善生物特性的关键优势。水凝胶在交联、模量和热稳定性方面的物理化学特性表明,PASCH 7:3 比其他组合更具优势。此外,事实证明混合蛋白水凝胶是一种有利的细胞基质,因为它能增强体外细胞的粘附、伸长、生长和增殖。延时显微镜研究显示,人类内皮细胞单层(EA.hy926)的伤口闭合能力得到了增强,而基因表达研究则描绘了细胞因子和生长因子在伤口环境中的动态相互作用,促进了细胞在蛋白质雕刻下的修复和再生。研究结果表明,制造的 PASCH 具有更好的物理和生物特性,体现了它们之间的协同作用,并表明它们可用于组织工程应用。
{"title":"Bioengineered Silk Fibroin Hydrogel Reinforced with Collagen-Like Protein Chimeras for Improved Wound Healing.","authors":"Thiagarajan Hemalatha, Mayilvahanan Aarthy, Ashokraj Sundarapandiyan, Niraikulam Ayyadurai","doi":"10.1002/mabi.202400346","DOIUrl":"https://doi.org/10.1002/mabi.202400346","url":null,"abstract":"<p><p>The study investigates the potentials of the rapid crosslinking hydrogel concoction comprising of natural silk fibroin (SF) and recombinant tailorable collagen-like protein with binding domains for wound repair. The formation of dityrosine crosslinks between the tyrosine moieties augments the formation of stable hydrogels, in the presence of the cytocompatible photo-initiator riboflavin and visible light. This uniquely engineered PASCH (Photo-activated silk fibroin and tailor-made collagen-like protein hydrogel) confers the key advantage of improved biological properties over the control hydrogels comprising only of SF. The physico-chemical characterization of the hydrogels with respect to crosslinking, modulus, and thermal stability delineates the ascendancy of PASCH 7:3 over other combinations. Furthermore, the hybrid protein hydrogel proves to be a favorable cellular matrix as it enhances cell adhesion, elongation, growth, and proliferation in vitro. Time-lapse microscopy studies reveal an enhanced wound closure in human endothelial cell monolayer (EA.hy926), while the gene expression studies portray the dynamic interplay of cytokines and growth factors in the wound milieu facilitating the repair and regeneration of cells, sculpted by the proteins. The results demonstrate the improved physical and biological properties of fabricated PASCH, depicting their synergism, and implying their competency for use in tissue engineering applications.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering. 运动中的分子:揭示组织工程中血管控制的动力学。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-18 DOI: 10.1002/mabi.202400139
Francisco A P Rodrigues, Cláudia S Oliveira, Simone C Sá, Freni K Tavaria, Sang Jin Lee, Ana L Oliveira, João B Costa

Significant progress has been made in tissue engineering (TE), aiming at providing personalized solutions and overcoming the current limitations of traditional tissue and organ transplantation. 3D bioprinting has emerged as a transformative technology in the field, able to mimic key properties of the natural architecture of the native tissues. However, most successes in the area are still limited to avascular or thin tissues due to the difficulties in controlling the vascularization of the engineered tissues. To address this issue, several molecules, biomaterials, and cells with pro- and anti-angiogenic potential have been intensively investigated. Furthermore, different bioreactors capable to provide a dynamic environment for in vitro vascularization control have been also explored. The present review summarizes the main molecules and TE strategies used to promote and inhibit vascularization in TE, as well as the techniques used to deliver them. Additionally, it also discusses the current challenges in 3D bioprinting and in tissue maturation to control in vitro/in vivo vascularization. Currently, this field of investigation is of utmost importance and may open doors for the design and development of more precise and controlled vascularization strategies in TE.

组织工程(TE)领域取得了重大进展,旨在提供个性化解决方案,克服传统组织和器官移植目前存在的局限性。三维生物打印技术已成为该领域的一项变革性技术,它能够模拟原生组织天然结构的关键特性。然而,由于难以控制工程组织的血管化,该领域的大多数成功案例仍局限于无血管或薄组织。为了解决这个问题,人们对一些具有促血管生成和抗血管生成潜能的分子、生物材料和细胞进行了深入研究。此外,人们还探索了能为体外血管生成控制提供动态环境的不同生物反应器。本综述总结了用于促进和抑制 TE 中血管生成的主要分子和 TE 策略,以及输送这些分子和策略的技术。此外,本综述还讨论了目前在三维生物打印和组织成熟过程中控制体外/体内血管化所面临的挑战。目前,这一研究领域极为重要,可能为设计和开发更精确、更可控的 TE 血管化策略打开大门。
{"title":"Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering.","authors":"Francisco A P Rodrigues, Cláudia S Oliveira, Simone C Sá, Freni K Tavaria, Sang Jin Lee, Ana L Oliveira, João B Costa","doi":"10.1002/mabi.202400139","DOIUrl":"https://doi.org/10.1002/mabi.202400139","url":null,"abstract":"<p><p>Significant progress has been made in tissue engineering (TE), aiming at providing personalized solutions and overcoming the current limitations of traditional tissue and organ transplantation. 3D bioprinting has emerged as a transformative technology in the field, able to mimic key properties of the natural architecture of the native tissues. However, most successes in the area are still limited to avascular or thin tissues due to the difficulties in controlling the vascularization of the engineered tissues. To address this issue, several molecules, biomaterials, and cells with pro- and anti-angiogenic potential have been intensively investigated. Furthermore, different bioreactors capable to provide a dynamic environment for in vitro vascularization control have been also explored. The present review summarizes the main molecules and TE strategies used to promote and inhibit vascularization in TE, as well as the techniques used to deliver them. Additionally, it also discusses the current challenges in 3D bioprinting and in tissue maturation to control in vitro/in vivo vascularization. Currently, this field of investigation is of utmost importance and may open doors for the design and development of more precise and controlled vascularization strategies in TE.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteins-Based Nanoparticles for Benznidazole Enteric Delivery. 基于蛋白质的苯并咪唑肠道给药纳米颗粒
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-18 DOI: 10.1002/mabi.202400338
Victor A Pilicita, Ana S Sonzogni, Mariana Allasia, Florencia Borra, Roque J Minari, Verónica D G Gonzalez

Chagas disease, caused by Trypanosoma cruzi (T. cruzi), affects millions worldwide, particularly in Latin America. Despite its prevalence, treatment options remain limited. Current drugs, such as benznidazole, cause adverse effects possibly due to ineffective administration. In this context, nanoparticles offer a promising solution to target and control drug delivery by leading the effector site and minimizing side effects. This article focuses on zein-casein-based nanoparticles (Bioparticles, BP) coated with Eudragit L100-55 (BP:EU) for enteric delivery of benznidazole. BP:EU structures are synthesized to minimize premature drug release in the stomach, promoting release in the small intestine. Physical characterization confirmed the successful synthesis of BP:EU and their pH-responsive trigger for drug release. These findings suggest that this material can be a promising approach for Chagas disease treatment, addressing challenges in benznidazole delivery that can lead to improved therapeutic responses.

由克鲁斯锥虫(T. cruzi)引起的恰加斯病影响着全球数百万人,尤其是拉丁美洲人。尽管该病很普遍,但治疗方案仍然有限。目前的药物(如苯并咪唑)可能会因用药不当而产生不良反应。在这种情况下,纳米颗粒提供了一种很有前景的解决方案,通过引导作用部位来靶向和控制给药,并最大限度地减少副作用。本文重点研究了涂有 Eudragit L100-55 (BP:EU)的玉米蛋白-酪蛋白基纳米颗粒(Bioparticles,BP),用于苯并咪唑的肠道给药。合成 BP:EU 结构可最大限度地减少药物在胃中的过早释放,促进药物在小肠中的释放。物理表征证实了 BP:EU 的成功合成及其释放药物的 pH 响应触发器。这些研究结果表明,这种材料是治疗南美锥虫病的一种很有前景的方法,它解决了苯并咪唑给药方面的难题,从而提高了治疗效果。
{"title":"Proteins-Based Nanoparticles for Benznidazole Enteric Delivery.","authors":"Victor A Pilicita, Ana S Sonzogni, Mariana Allasia, Florencia Borra, Roque J Minari, Verónica D G Gonzalez","doi":"10.1002/mabi.202400338","DOIUrl":"https://doi.org/10.1002/mabi.202400338","url":null,"abstract":"<p><p>Chagas disease, caused by Trypanosoma cruzi (T. cruzi), affects millions worldwide, particularly in Latin America. Despite its prevalence, treatment options remain limited. Current drugs, such as benznidazole, cause adverse effects possibly due to ineffective administration. In this context, nanoparticles offer a promising solution to target and control drug delivery by leading the effector site and minimizing side effects. This article focuses on zein-casein-based nanoparticles (Bioparticles, BP) coated with Eudragit L100-55 (BP:EU) for enteric delivery of benznidazole. BP:EU structures are synthesized to minimize premature drug release in the stomach, promoting release in the small intestine. Physical characterization confirmed the successful synthesis of BP:EU and their pH-responsive trigger for drug release. These findings suggest that this material can be a promising approach for Chagas disease treatment, addressing challenges in benznidazole delivery that can lead to improved therapeutic responses.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study. 在组织工程中开发脱细胞细胞外基质 (ECM) 的潜力:回顾研究。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-16 DOI: 10.1002/mabi.202400322
Peiman Brouki Milan, Farimah Masoumi, Esmaeil Biazar, Saeedeh Zare Jalise, Arezou Mehrabi

While significant progress has been made in creating polymeric structures for tissue engineering, the therapeutic application of these scaffolds remains challenging owing to the intricate nature of replicating the conditions of native organs and tissues. The use of human-derived biomaterials for therapeutic purposes closely imitates the properties of natural tissue, thereby assisting in tissue regeneration. Decellularized extracellular matrix (dECM) scaffolds derived from natural tissues have become popular because of their unique biomimetic properties. These dECM scaffolds can enhance the body's ability to heal itself or be used to generate new tissues for restoration, expanding beyond traditional tissue transfers and transplants. Enhanced knowledge of how ECM scaffold materials affect the microenvironment at the injury site is expected to improve clinical outcomes. In this review, recent advancements in dECM scaffolds are explored and relevant perspectives are offered, highlighting the development and application of these scaffolds in tissue engineering for various organs, such as the skin, nerve, bone, heart, liver, lung, and kidney.

虽然在为组织工程创造聚合物结构方面取得了重大进展,但由于复制天然器官和组织条件的复杂性,这些支架的治疗应用仍具有挑战性。将源自人体的生物材料用于治疗目的,可以近似地模仿天然组织的特性,从而帮助组织再生。源自天然组织的脱细胞细胞外基质(dECM)支架因其独特的生物模拟特性而广受欢迎。这些脱细胞细胞外基质支架可增强人体的自愈能力,或用于生成新的组织进行修复,超越了传统的组织转移和移植。加强对 ECM 支架材料如何影响损伤部位微环境的了解有望改善临床效果。本综述探讨了 dECM 支架的最新进展并提供了相关观点,重点介绍了这些支架在皮肤、神经、骨骼、心脏、肝脏、肺和肾脏等各种器官的组织工程中的开发和应用。
{"title":"Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study.","authors":"Peiman Brouki Milan, Farimah Masoumi, Esmaeil Biazar, Saeedeh Zare Jalise, Arezou Mehrabi","doi":"10.1002/mabi.202400322","DOIUrl":"https://doi.org/10.1002/mabi.202400322","url":null,"abstract":"<p><p>While significant progress has been made in creating polymeric structures for tissue engineering, the therapeutic application of these scaffolds remains challenging owing to the intricate nature of replicating the conditions of native organs and tissues. The use of human-derived biomaterials for therapeutic purposes closely imitates the properties of natural tissue, thereby assisting in tissue regeneration. Decellularized extracellular matrix (dECM) scaffolds derived from natural tissues have become popular because of their unique biomimetic properties. These dECM scaffolds can enhance the body's ability to heal itself or be used to generate new tissues for restoration, expanding beyond traditional tissue transfers and transplants. Enhanced knowledge of how ECM scaffold materials affect the microenvironment at the injury site is expected to improve clinical outcomes. In this review, recent advancements in dECM scaffolds are explored and relevant perspectives are offered, highlighting the development and application of these scaffolds in tissue engineering for various organs, such as the skin, nerve, bone, heart, liver, lung, and kidney.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Magnetically Active Bicontinuous Polymer Structures for Multiple Controlled Drug Delivery 用于多重可控给药的磁活性双连续聚合物结构
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-15 DOI: 10.1002/mabi.202470025
Elisa Lacroce, Fabio Pizzetti, Nicolás M. Barbosa Urrego, Giuseppe Nunziata, Maurizio Masi, Filippo Rossi

Back Cover: In article 2400084, Filippo Rossi and co-workers develop a magnetic bijel-like structure to load and release different types of molecules (hydrophilic and hydrophobic). The use of ε-caprolactone is explored, which can polymerize, forming hydrophobic domains (oil phase). After mixing with iron oxide nanoparticles (NPs), the water dispersion creates a magnetic biphasic porous structure without phase separation.

封底:在文章 2400084 中,Filippo Rossi 及其合作者开发了一种类似磁性 bijel 的结构,用于装载和释放不同类型的分子(亲水性和疏水性)。他们探索了ε-己内酯的使用,这种物质可以聚合,形成疏水域(油相)。在与氧化铁纳米粒子(NPs)混合后,水分散体会形成一种磁性双相多孔结构,且不会发生相分离。
{"title":"Magnetically Active Bicontinuous Polymer Structures for Multiple Controlled Drug Delivery","authors":"Elisa Lacroce,&nbsp;Fabio Pizzetti,&nbsp;Nicolás M. Barbosa Urrego,&nbsp;Giuseppe Nunziata,&nbsp;Maurizio Masi,&nbsp;Filippo Rossi","doi":"10.1002/mabi.202470025","DOIUrl":"https://doi.org/10.1002/mabi.202470025","url":null,"abstract":"<p><b>Back Cover</b>: In article 2400084, Filippo Rossi and co-workers develop a magnetic bijel-like structure to load and release different types of molecules (hydrophilic and hydrophobic). The use of ε-caprolactone is explored, which can polymerize, forming hydrophobic domains (oil phase). After mixing with iron oxide nanoparticles (NPs), the water dispersion creates a magnetic biphasic porous structure without phase separation.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mineralized Biopolymers-Based Scaffold Encapsulating with Dual Drugs for Alveolar Ridge Preservation. 基于矿化生物聚合物的支架,包裹双重药物用于牙槽嵴保留。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-15 DOI: 10.1002/mabi.202400351
Thanh-Han Hoang Tran, Cuong Hung Luu, Khanh-Tram Thi Nguyen, Mai-Anh Le Hoang, Quang-Khanh Pham, Chau My Phan, Nguyen-Kim-Luong Thai, Hieu Trung Nguyen, Thavasyappan Thambi, V H Giang Phan

Mineralization of scaffolds is essential for alveolar ridge preservation and bone tissue engineering, enhancing the mechanical strength and bioactivity of scaffolds, and promoting better integration with natural bone tissue. While the in situ mineralization method using concentrated SBF solutions is promising, there is limited comprehensive research on its effects. In this study, it is demonstrate that soaking gelatin/alginate scaffolds (GAS) in fivefold concentrated SBF significantly reduces the mineralization time to 3-7 days but also leads to considerable degradation and loss of the scaffold's original microstructure. The ratio of gelatin to alginate is optimized to improve the properties of GAS. The optimized GAS sample, when soaked in concentrated SBF to form GAS/HAp, exhibited hydroxyapatite (HAp) crystal formation starting from day 3, with mature hexagonal crystals forming by day 7. However, this process also caused significant decomposition and deformation of the scaffold's pore structure. Additionally, the biocompatibility of GAS and GAS/HAp is evaluated through in vitro, in ovo, haemolysis, and anti-ROS assays. The findings highlight the impact of SBF on the mineralization of GAS, laying the groundwork for further research in alveolar ridge preservation and bone tissue engineering.

支架的矿化对于牙槽嵴保存和骨组织工程至关重要,它能增强支架的机械强度和生物活性,促进与天然骨组织更好地融合。虽然使用浓缩 SBF 溶液进行原位矿化的方法很有前景,但对其效果的全面研究却很有限。本研究表明,将明胶/海藻酸盐支架(GAS)浸泡在五倍浓缩的 SBF 溶液中可将矿化时间显著缩短至 3-7 天,但同时也会导致相当程度的降解和支架原有微观结构的丧失。优化明胶与海藻酸盐的比例可改善 GAS 的性能。优化后的 GAS 样品在浓 SBF 中浸泡形成 GAS/HAp 后,从第 3 天开始出现羟基磷灰石(HAp)晶体形成,到第 7 天形成成熟的六方晶体。然而,这一过程也会导致支架孔隙结构发生明显的分解和变形。此外,还通过体外、体内、溶血和抗 ROS 检测评估了 GAS 和 GAS/HAp 的生物相容性。研究结果强调了 SBF5× 对 GAS 矿化的影响,为进一步研究牙槽嵴保存和骨组织工程奠定了基础。
{"title":"Mineralized Biopolymers-Based Scaffold Encapsulating with Dual Drugs for Alveolar Ridge Preservation.","authors":"Thanh-Han Hoang Tran, Cuong Hung Luu, Khanh-Tram Thi Nguyen, Mai-Anh Le Hoang, Quang-Khanh Pham, Chau My Phan, Nguyen-Kim-Luong Thai, Hieu Trung Nguyen, Thavasyappan Thambi, V H Giang Phan","doi":"10.1002/mabi.202400351","DOIUrl":"https://doi.org/10.1002/mabi.202400351","url":null,"abstract":"<p><p>Mineralization of scaffolds is essential for alveolar ridge preservation and bone tissue engineering, enhancing the mechanical strength and bioactivity of scaffolds, and promoting better integration with natural bone tissue. While the in situ mineralization method using concentrated SBF solutions is promising, there is limited comprehensive research on its effects. In this study, it is demonstrate that soaking gelatin/alginate scaffolds (GAS) in fivefold concentrated SBF significantly reduces the mineralization time to 3-7 days but also leads to considerable degradation and loss of the scaffold's original microstructure. The ratio of gelatin to alginate is optimized to improve the properties of GAS. The optimized GAS sample, when soaked in concentrated SBF to form GAS/HAp, exhibited hydroxyapatite (HAp) crystal formation starting from day 3, with mature hexagonal crystals forming by day 7. However, this process also caused significant decomposition and deformation of the scaffold's pore structure. Additionally, the biocompatibility of GAS and GAS/HAp is evaluated through in vitro, in ovo, haemolysis, and anti-ROS assays. The findings highlight the impact of SBF<sub>5×</sub> on the mineralization of GAS, laying the groundwork for further research in alveolar ridge preservation and bone tissue engineering.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Injectable Photothermal PDA/Chitosan/β-Glycerophosphate Thermosensitive Hydrogels for Antibacterial and Wound Healing Promotion 用于抗菌和促进伤口愈合的可注射光热 PDA/壳聚糖/β-甘油磷酸酯热敏水凝胶
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-15 DOI: 10.1002/mabi.202470023
Dingkun Liu, Jinbing Chen, Linjuan Gao, Xing Chen, Liujun Lin, Xia Wei, Yuan Liu, Hui Cheng

Front Cover: In article 2400080, Hui Cheng and co-workers introduce a temperature-sensitive hydrogel with photothermal conversion capabilities. The cover shows how the hydrogel works. The temperature-sensitive hydrogel is injected to fill the entire wound, and then excited by near-infrared light, the hydrogel exerts a photothermal effect, raising the temperature to kill bacteria and ultimately promoting tissue healing.

封面:在文章 2400080 中,Hui Cheng 及其合作者介绍了一种具有光热转换功能的温敏水凝胶。封面展示了水凝胶的工作原理。将温敏水凝胶注入伤口,使其充满整个伤口,然后在近红外线的激发下,水凝胶产生光热效应,使温度升高以杀死细菌,最终促进组织愈合。
{"title":"Injectable Photothermal PDA/Chitosan/β-Glycerophosphate Thermosensitive Hydrogels for Antibacterial and Wound Healing Promotion","authors":"Dingkun Liu,&nbsp;Jinbing Chen,&nbsp;Linjuan Gao,&nbsp;Xing Chen,&nbsp;Liujun Lin,&nbsp;Xia Wei,&nbsp;Yuan Liu,&nbsp;Hui Cheng","doi":"10.1002/mabi.202470023","DOIUrl":"https://doi.org/10.1002/mabi.202470023","url":null,"abstract":"<p><b>Front Cover</b>: In article 2400080, Hui Cheng and co-workers introduce a temperature-sensitive hydrogel with photothermal conversion capabilities. The cover shows how the hydrogel works. The temperature-sensitive hydrogel is injected to fill the entire wound, and then excited by near-infrared light, the hydrogel exerts a photothermal effect, raising the temperature to kill bacteria and ultimately promoting tissue healing.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Masthead: Macromol. Biosci. 10/2024 刊头:Macromol.Biosci.10/2024
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-15 DOI: 10.1002/mabi.202470024
{"title":"Masthead: Macromol. Biosci. 10/2024","authors":"","doi":"10.1002/mabi.202470024","DOIUrl":"https://doi.org/10.1002/mabi.202470024","url":null,"abstract":"","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cationic Carbosilane Dendrimers for Apmnkq2 Aptamer Transfection in Breast Cancer: An Alternative to Traditional Transfectants. 阳离子碳硅烷树枝状聚合物用于乳腺癌的 Apmnkq2 Aptamer 转染:传统转染剂的替代品
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-14 DOI: 10.1002/mabi.202400327
Laura Madrid, Celia Pinto-Díez, Laura Muñoz-Moreno, Marta García-Hernández, Víctor M González, Javier de la Mata, M Elena Martin, Paula Ortega

Transfection efficiency is a critical parameter in gene therapy and molecular biology, representing the success rate at which nucleic acids are introduced and expressed in target cells. The combination of aptamers with nanotechnology-based delivery systems has demonstrated remarkable improvements in the transfection efficiency of therapeutic agents and holds significant potential for advancing gene therapy and the development of targeted treatments for various diseases, including cancer. In this work, cationic carbosilane dendritic systems are presented as an alternative to commercial transfection agents, demonstrating an increase in transfection efficiency when used for the internalization of apMNKQ2, an aptamer selected against a target in cancer. Their potential therapeutic use has been evaluated in breast cancer cell lines, MDA-MB-468 and MDA-MB-231, studying the cytotoxicity of the nanoconjugate, the internalization process, and its effect on cellular migration processes.

转染效率是基因治疗和分子生物学中的一个关键参数,代表着核酸在靶细胞中导入和表达的成功率。将适配体与基于纳米技术的递送系统相结合,可显著提高治疗剂的转染效率,在推进基因治疗和开发包括癌症在内的各种疾病的靶向治疗方面具有巨大潜力。在这项研究中,阳离子碳硅烷树枝状系统被用作商业转染剂的替代品,当用于内化针对癌症靶点的apMNKQ2时,显示出转染效率的提高。在乳腺癌细胞系 MDA-MB-468 和 MDA-MB-231 中对其潜在的治疗用途进行了评估,研究了纳米共轭物的细胞毒性、内化过程及其对细胞迁移过程的影响。
{"title":"Cationic Carbosilane Dendrimers for Apmnkq2 Aptamer Transfection in Breast Cancer: An Alternative to Traditional Transfectants.","authors":"Laura Madrid, Celia Pinto-Díez, Laura Muñoz-Moreno, Marta García-Hernández, Víctor M González, Javier de la Mata, M Elena Martin, Paula Ortega","doi":"10.1002/mabi.202400327","DOIUrl":"https://doi.org/10.1002/mabi.202400327","url":null,"abstract":"<p><p>Transfection efficiency is a critical parameter in gene therapy and molecular biology, representing the success rate at which nucleic acids are introduced and expressed in target cells. The combination of aptamers with nanotechnology-based delivery systems has demonstrated remarkable improvements in the transfection efficiency of therapeutic agents and holds significant potential for advancing gene therapy and the development of targeted treatments for various diseases, including cancer. In this work, cationic carbosilane dendritic systems are presented as an alternative to commercial transfection agents, demonstrating an increase in transfection efficiency when used for the internalization of apMNKQ2, an aptamer selected against a target in cancer. Their potential therapeutic use has been evaluated in breast cancer cell lines, MDA-MB-468 and MDA-MB-231, studying the cytotoxicity of the nanoconjugate, the internalization process, and its effect on cellular migration processes.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Facile Strategy for PEGylated Nanoprodrug of Bortezomib with Improved Stability, Enhanced Biocompatibility, pH-Controlled Disassembly, and Release. 硼替佐米 PEG 化纳米药物的简便策略,具有更高的稳定性、更强的生物相容性、pH 值可控的分解和释放。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-14 DOI: 10.1002/mabi.202400383
Naikuan Fu, Yinan Zeng, Jing Zhang, Peng Zhang, Hong Zhang, Shicheng Yang, Jianhua Zhang

The therapeutic efficacy of bortezomib (BTZ) is often limited due to low solubility, poor stability in vivo and nonspecific toxicity. Herein, a kind of catechol-functionalized polyethylene glycol (mPEG-CA) is first synthesized and then mPEG-CA is readily used to conjugate with BTZ by the formation of dynamic boronate bonds to obtain PEGlyated BTZ prodrug (mPEG-CA-BTZ) with the ability of pH-controlled disassembly and drug release. The structure and morphology, physicochemical characteristics, drug loading, and release as well as in vitro cytotoxicity of mPEG-CA-BTZ nanoparticles are investigated in detail. The results demonstrated that mPEG-CA-BTZ can not only self-assemble into nanostructures with uniform size and stable dispersion in physiological pH condition (pH 7.4) but also respond to the tumor acid microenvironment and achieve pH-controlled BTZ release by acid-triggered cleavage of boronate bonds, decomposition of mPEG-CA-BTZ and thus disassembly of mPEG-CA-BTZ nanoparticles. mPEG-CA-BTZ nanoparticles are expected to have great potential as a promising nanoplatform for pharmaceutical formulations of BTZ to increase therapeutic efficacy and decrease side effects of BTZ. Considering the easily available and biocompatible excipients and simple preparation process, the strategy designed herein provides a facile and promising approach to synergistically integrate the function of PEGylation and pH-sensitiveness into boronic acid-containing small molecule pharmaceutical agents.

硼替佐米(BTZ)由于溶解度低、体内稳定性差以及非特异性毒性,其疗效往往受到限制。本文首先合成了一种儿茶酚官能化聚乙二醇(mPEG-CA),然后利用mPEG-CA与硼替佐米(BTZ)形成动态硼酸键,获得了具有pH可控拆分和药物释放能力的PEG化硼替佐米原药(mPEG-CA-BTZ)。研究人员详细探讨了 mPEG-CA-BTZ 纳米颗粒的结构和形态、理化特性、药物负载和释放以及体外细胞毒性。结果表明,mPEG-CA-BTZ不仅能在生理pH值(pH 7.4)条件下自组装成大小均匀、分散稳定的纳米结构,还能对肿瘤酸性微环境做出反应,通过酸触发硼酸键裂解、mPEG-CA-BTZ分解进而解体mPEG-CA-BTZ纳米颗粒,实现pH值控制的BTZ释放。mPEG-CA-BTZ 纳米颗粒有望成为 BTZ 药物制剂的一个前景广阔的纳米平台,从而提高 BTZ 的疗效并减少其副作用。考虑到辅料的易得性和生物相容性以及制备过程的简便性,本文设计的策略为将 PEG 化功能和 pH 敏性协同整合到含硼酸的小分子药物中提供了一种简便而有前景的方法。
{"title":"A Facile Strategy for PEGylated Nanoprodrug of Bortezomib with Improved Stability, Enhanced Biocompatibility, pH-Controlled Disassembly, and Release.","authors":"Naikuan Fu, Yinan Zeng, Jing Zhang, Peng Zhang, Hong Zhang, Shicheng Yang, Jianhua Zhang","doi":"10.1002/mabi.202400383","DOIUrl":"https://doi.org/10.1002/mabi.202400383","url":null,"abstract":"<p><p>The therapeutic efficacy of bortezomib (BTZ) is often limited due to low solubility, poor stability in vivo and nonspecific toxicity. Herein, a kind of catechol-functionalized polyethylene glycol (mPEG-CA) is first synthesized and then mPEG-CA is readily used to conjugate with BTZ by the formation of dynamic boronate bonds to obtain PEGlyated BTZ prodrug (mPEG-CA-BTZ) with the ability of pH-controlled disassembly and drug release. The structure and morphology, physicochemical characteristics, drug loading, and release as well as in vitro cytotoxicity of mPEG-CA-BTZ nanoparticles are investigated in detail. The results demonstrated that mPEG-CA-BTZ can not only self-assemble into nanostructures with uniform size and stable dispersion in physiological pH condition (pH 7.4) but also respond to the tumor acid microenvironment and achieve pH-controlled BTZ release by acid-triggered cleavage of boronate bonds, decomposition of mPEG-CA-BTZ and thus disassembly of mPEG-CA-BTZ nanoparticles. mPEG-CA-BTZ nanoparticles are expected to have great potential as a promising nanoplatform for pharmaceutical formulations of BTZ to increase therapeutic efficacy and decrease side effects of BTZ. Considering the easily available and biocompatible excipients and simple preparation process, the strategy designed herein provides a facile and promising approach to synergistically integrate the function of PEGylation and pH-sensitiveness into boronic acid-containing small molecule pharmaceutical agents.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Macromolecular bioscience
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1