首页 > 最新文献

Macromolecular bioscience最新文献

英文 中文
Flexible, Electrochemical Skin‐Like Platform for Inflammatory Biomarker Monitoring 用于炎症生物标记物监测的灵活电化学类肤平台
IF 4.6 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-18 DOI: 10.1002/mabi.202400287
Carolina Lourenço, Felismina Moreira, Rui Igreja, Gabriela Martins
Addressing global challenges in wound management has greatly encouraged the emergence of home diagnosis and monitoring devices. This technological shift has accelerated the development of new skin patch sensors for continuous health monitoring. A key requirement is the creation of flexible platforms capable of mimicking human skin features. Here, for the first time, an innovative, highly adaptable electrochemical biosensor with molecularly imprinted polymers (MIPs) is customized for the detection of the inflammatory biomarker interleukin‐6 (IL‐6). The 3‐electrode gold pattern is geometrically standardized onto a 6 µm thick polyimide flexible membrane, an optically transparent, and biocompatible polymeric substrate. Subsequently, a biomimetic sensing layer specifically designed for the detection of IL‐6 target is produced on these transducers. The obtained MIP biosensor shows a good linear response within the concentration range 50 pg mL−1‐50 ng mL−1, with a low limit of detection (8 pg mL−1). X‐ray photoelectron spectroscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy characterizations confirm the modifications of the flexible gold transducer. After optimization, the biosensing device shows remarkable potential in terms of sensitivity, selectivity, and reproducibility. Overall, the integration of a low‐cost electrochemical sensor on biocompatible flexible polymers opens the way for a new generation of monitoring tools with higher accuracy, less invasiveness, and greater patient comfort.
应对全球伤口管理方面的挑战极大地促进了家庭诊断和监测设备的出现。这一技术转变加速了用于持续健康监测的新型皮肤贴片传感器的开发。一个关键的要求是创建能够模仿人体皮肤特征的灵活平台。在这里,我们首次利用分子印迹聚合物(MIPs)定制了一种创新的、适应性强的电化学生物传感器,用于检测炎症生物标志物白细胞介素-6(IL-6)。在 6 微米厚的聚酰亚胺柔性膜(一种光学透明、生物兼容的聚合物基底)上,对 3 个电极的金图案进行了几何标准化。随后,在这些传感器上制作了专为检测 IL-6 目标而设计的仿生物传感层。获得的 MIP 生物传感器在 50 pg mL-1-50 ng mL-1 的浓度范围内显示出良好的线性响应,检测限低(8 pg mL-1)。X 射线光电子能谱、扫描电子显微镜和傅立叶变换红外光谱表征证实了柔性金传感器的改性。经过优化后,该生物传感装置在灵敏度、选择性和再现性方面都显示出显著的潜力。总之,在生物相容性柔性聚合物上集成低成本电化学传感器,为开发新一代监测工具开辟了道路,这种监测工具具有更高的准确性、更低的侵入性和更高的病人舒适度。
{"title":"Flexible, Electrochemical Skin‐Like Platform for Inflammatory Biomarker Monitoring","authors":"Carolina Lourenço, Felismina Moreira, Rui Igreja, Gabriela Martins","doi":"10.1002/mabi.202400287","DOIUrl":"https://doi.org/10.1002/mabi.202400287","url":null,"abstract":"Addressing global challenges in wound management has greatly encouraged the emergence of home diagnosis and monitoring devices. This technological shift has accelerated the development of new skin patch sensors for continuous health monitoring. A key requirement is the creation of flexible platforms capable of mimicking human skin features. Here, for the first time, an innovative, highly adaptable electrochemical biosensor with molecularly imprinted polymers (MIPs) is customized for the detection of the inflammatory biomarker interleukin‐6 (IL‐6). The 3‐electrode gold pattern is geometrically standardized onto a 6 µm thick polyimide flexible membrane, an optically transparent, and biocompatible polymeric substrate. Subsequently, a biomimetic sensing layer specifically designed for the detection of IL‐6 target is produced on these transducers. The obtained MIP biosensor shows a good linear response within the concentration range 50 pg mL<jats:sup>−1</jats:sup>‐50 ng mL<jats:sup>−1</jats:sup>, with a low limit of detection (8 pg mL<jats:sup>−1</jats:sup>). X‐ray photoelectron spectroscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy characterizations confirm the modifications of the flexible gold transducer. After optimization, the biosensing device shows remarkable potential in terms of sensitivity, selectivity, and reproducibility. Overall, the integration of a low‐cost electrochemical sensor on biocompatible flexible polymers opens the way for a new generation of monitoring tools with higher accuracy, less invasiveness, and greater patient comfort.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Masthead: Macromol. Biosci. 9/2024 刊头:Macromol.Biosci.9/2024
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470021
{"title":"Masthead: Macromol. Biosci. 9/2024","authors":"","doi":"10.1002/mabi.202470021","DOIUrl":"https://doi.org/10.1002/mabi.202470021","url":null,"abstract":"","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142244756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viral Vector Eluting Lenses for Single-Step Targeted Expression of Genetically-Encoded Activity Sensors for in Vivo Microendoscopic Calcium Imaging. 用于体内显微内窥镜钙成像的基因编码活动传感器单步靶向表达的病毒载体洗脱透镜。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-16 DOI: 10.1002/mabi.202400359
Carolyn K Jons, David Cheng, Changxin Dong, Emily L Meany, Jonathan J Nassi, Eric A Appel

Optical methods for studying the brain offer powerful approaches for understanding how neural activity underlies complex behavior. These methods typically rely on genetically encoded sensors and actuators to monitor and control neural activity. For microendoscopic calcium imaging, injection of a virus followed by implantation of a lens probe is required to express a calcium sensor and enable optical access to the target brain region. This two-step process poses several challenges, chief among them being the risks associated with mistargeting and/or misalignment between virus expression zone, lens probe and target brain region. Here, an adeno-associated virus (AAV)-eluting polymer coating is engineered for gradient refractive index (GRIN) lenses enabling the expression of a genetically encoded calcium indicator (GCaMP) directly within the brain region of interest upon implantation of the lens. This approach requires only one surgical step and guarantees alignment between GCaMP expression and lens in the brain. Additionally, the slow virus release from these coatings increases the working time for surgical implantation, expanding the brain regions and species amenable to this approach. These enhanced capabilities should accelerate neuroscience research utilizing optical methods and advance the understanding of the neural circuit mechanisms underlying brain function and behavior in health and disease.

研究大脑的光学方法为了解神经活动如何成为复杂行为的基础提供了强有力的方法。这些方法通常依靠基因编码的传感器和致动器来监测和控制神经活动。在微内窥镜钙成像中,需要先注射病毒,然后植入透镜探针,以表达钙传感器,并通过光学方法进入目标脑区。这两个步骤带来了一些挑战,其中最主要的是与病毒表达区、透镜探针和目标脑区之间的误定位和/或错位有关的风险。在这里,为梯度折射率(GRIN)镜片设计了一种腺相关病毒(AAV)洗脱聚合物涂层,可在植入镜片后直接在相关脑区表达基因编码的钙指示剂(GCaMP)。这种方法只需一个手术步骤,并能保证 GCaMP 的表达与透镜在大脑中的位置一致。此外,这些涂层释放病毒的速度很慢,因此增加了手术植入的工作时间,扩大了适合这种方法的大脑区域和物种。这些增强的功能将加速利用光学方法进行的神经科学研究,并促进对大脑功能和健康与疾病行为的神经回路机制的了解。
{"title":"Viral Vector Eluting Lenses for Single-Step Targeted Expression of Genetically-Encoded Activity Sensors for in Vivo Microendoscopic Calcium Imaging.","authors":"Carolyn K Jons, David Cheng, Changxin Dong, Emily L Meany, Jonathan J Nassi, Eric A Appel","doi":"10.1002/mabi.202400359","DOIUrl":"10.1002/mabi.202400359","url":null,"abstract":"<p><p>Optical methods for studying the brain offer powerful approaches for understanding how neural activity underlies complex behavior. These methods typically rely on genetically encoded sensors and actuators to monitor and control neural activity. For microendoscopic calcium imaging, injection of a virus followed by implantation of a lens probe is required to express a calcium sensor and enable optical access to the target brain region. This two-step process poses several challenges, chief among them being the risks associated with mistargeting and/or misalignment between virus expression zone, lens probe and target brain region. Here, an adeno-associated virus (AAV)-eluting polymer coating is engineered for gradient refractive index (GRIN) lenses enabling the expression of a genetically encoded calcium indicator (GCaMP) directly within the brain region of interest upon implantation of the lens. This approach requires only one surgical step and guarantees alignment between GCaMP expression and lens in the brain. Additionally, the slow virus release from these coatings increases the working time for surgical implantation, expanding the brain regions and species amenable to this approach. These enhanced capabilities should accelerate neuroscience research utilizing optical methods and advance the understanding of the neural circuit mechanisms underlying brain function and behavior in health and disease.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
One Stone Several Birds: Self-Localizing Submicrocages With Dual Loading Points for Multifunctional Drug Delivery 一石数鸟:具有双装载点的自定位亚微囊用于多功能药物输送
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470020
Shuxuan Liu, Jifei Wang, Yong Jiang, Yao Wang, Bin Yang, Hao Li, Guofu Zhou

Front Cover: The idea for the cover image comes from a famous Chinese fairy tale, “Nezha Conquers the Dragon King”. In this picture, Nezha with three heads and six arms represents the multifunctional submicrocage. Nezha will fight a huge dragon ball, namely “tumor”, spit out by Dragon King. More details can be found in article 2400033 by Bin Yang, Hao Li, and co-workers.

封面封面图片的创意来自中国著名童话故事《哪吒闹海》。在这幅图中,三头六臂的哪吒代表了多功能亚微型笼。哪吒将与龙王吐出的巨大龙珠 "毒瘤 "作战。更多详情,请参阅杨斌、李浩及合作者撰写的文章 2400033。
{"title":"One Stone Several Birds: Self-Localizing Submicrocages With Dual Loading Points for Multifunctional Drug Delivery","authors":"Shuxuan Liu,&nbsp;Jifei Wang,&nbsp;Yong Jiang,&nbsp;Yao Wang,&nbsp;Bin Yang,&nbsp;Hao Li,&nbsp;Guofu Zhou","doi":"10.1002/mabi.202470020","DOIUrl":"https://doi.org/10.1002/mabi.202470020","url":null,"abstract":"<p><b>Front Cover</b>: The idea for the cover image comes from a famous Chinese fairy tale, “Nezha Conquers the Dragon King”. In this picture, Nezha with three heads and six arms represents the multifunctional submicrocage. Nezha will fight a huge dragon ball, namely “tumor”, spit out by Dragon King. More details can be found in article 2400033 by Bin Yang, Hao Li, and co-workers.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142244514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unlocking the Potential: PEGylation and Molecular Weight Reduction of Ionenes for Enhanced Antifungal Activity and Biocompatibility 释放潜力:离子烯的 PEG 化和分子量降低可增强抗真菌活性和生物相容性
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470022
Jan M. Kurzyna, Rafał J. Kopiasz, Martyna Paul, Magdalena Flont, Patrycja Baranowska, Jolanta Mierzejewska, Karolina Drężek, Waldemar Tomaszewski, Elżbieta Jastrzębska, Dominik Jańczewski

Back Cover: Synthetic polymers mimicking antimicrobial peptides show potent activity but high toxicity. This study's cationic ionenes, modified with a PEG side chain, exhibited high antimicrobial activity and reduced toxicity. Remarkably, lower molecular weight increased antifungal activity, with MICs as low as 2 and 0.0625 µg/mL for C. albicans and C. tropicalis. More details can be found in article 2400032 by Dominik Jańczewski and co-workers.

封底:模仿抗菌肽的合成聚合物具有强效活性,但毒性较高。这项研究的阳离子烯经 PEG 侧链修饰后,显示出很高的抗菌活性,同时毒性降低。值得注意的是,分子量越低,抗真菌活性越强,对白僵菌和热带僵菌的 MIC 值分别低至 2 微克/毫升和 0.0625 微克/毫升。更多详情,请参阅 Dominik Jańczewski 及其合作者撰写的文章 2400032。
{"title":"Unlocking the Potential: PEGylation and Molecular Weight Reduction of Ionenes for Enhanced Antifungal Activity and Biocompatibility","authors":"Jan M. Kurzyna,&nbsp;Rafał J. Kopiasz,&nbsp;Martyna Paul,&nbsp;Magdalena Flont,&nbsp;Patrycja Baranowska,&nbsp;Jolanta Mierzejewska,&nbsp;Karolina Drężek,&nbsp;Waldemar Tomaszewski,&nbsp;Elżbieta Jastrzębska,&nbsp;Dominik Jańczewski","doi":"10.1002/mabi.202470022","DOIUrl":"https://doi.org/10.1002/mabi.202470022","url":null,"abstract":"<p><b>Back Cover</b>: Synthetic polymers mimicking antimicrobial peptides show potent activity but high toxicity. This study's cationic ionenes, modified with a PEG side chain, exhibited high antimicrobial activity and reduced toxicity. Remarkably, lower molecular weight increased antifungal activity, with MICs as low as 2 and 0.0625 µg/mL for <i>C. albicans</i> and <i>C. tropicalis</i>. More details can be found in article 2400032 by Dominik Jańczewski and co-workers.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202470022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142244525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preparation, Structural Analysis, and Growth‐Promoting Effects of Amomum longiligulare Polysaccharide 1‐Mg (II) Complex 长日月藻多糖 1-Mg (II) 复合物的制备、结构分析和促进生长作用
IF 4.6 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-13 DOI: 10.1002/mabi.202400297
Yijing Chen, Haowen Wu, Chenglong Zhang, Qiyuan Luo, Yun Chen
In this study, Amomum longiligulare polysaccharide 1 (ALP1) is used to chelate with magnesium (Mg) to synthesize the ALP1‐Mg (II) complex (ALP1‐Mg). Based on Box‐Behnken response surface design, the optimum technological conditions are 22 mg mL−1 trisodium citrate, 2.10 mol L−1 MgCl2, reaction at 70 °C for 2.9 h, resulting in a maximum Mg content of 2.13%. Next, the physicochemical properties and structural characteristics of ALP1 and ALP1‐Mg are characterized, and the results show that the morphology, conformation, crystallinity, and thermal stability of ALP1‐Mg are changed. In addition, dietary supplementation of 500 mg kg−1 ALP1‐Mg significantly reduces the feed conversion ratio during the grower (15–35 d). Meanwhile, the villus height/crypt depth of the duodenum and ileum are significantly increased, and the relative abundance of Lactobacillus is significantly elevated. Taken together, the results suggest that ALP1‐Mg is a potential growth‐promoting feed additive.
本研究利用Amomum longiligulare多糖1(ALP1)与镁(Mg)螯合,合成ALP1-Mg(II)复合物(ALP1-Mg)。根据 Box-Behnken 响应面设计,最佳工艺条件为 22 mg mL-1 柠檬酸三钠、2.10 mol L-1 MgCl2、70 ℃ 反应 2.9 h,镁含量最高为 2.13%。接着,对 ALP1 和 ALP1-Mg 的理化性质和结构特征进行了表征,结果表明 ALP1-Mg 的形态、构象、结晶度和热稳定性都发生了变化。此外,日粮中添加 500 mg kg-1 ALP1-Mg 会显著降低生长期(15-35 d)的饲料转化率。同时,十二指肠和回肠的绒毛高度/绒毛深度明显增加,乳酸杆菌的相对丰度明显提高。综上所述,这些结果表明 ALP1-Mg 是一种潜在的促生长饲料添加剂。
{"title":"Preparation, Structural Analysis, and Growth‐Promoting Effects of Amomum longiligulare Polysaccharide 1‐Mg (II) Complex","authors":"Yijing Chen, Haowen Wu, Chenglong Zhang, Qiyuan Luo, Yun Chen","doi":"10.1002/mabi.202400297","DOIUrl":"https://doi.org/10.1002/mabi.202400297","url":null,"abstract":"In this study, <jats:italic>Amomum longiligulare</jats:italic> polysaccharide 1 (ALP1) is used to chelate with magnesium (Mg) to synthesize the ALP1‐Mg (II) complex (ALP1‐Mg). Based on Box‐Behnken response surface design, the optimum technological conditions are 22 mg mL<jats:sup>−1</jats:sup> trisodium citrate, 2.10 mol L<jats:sup>−1</jats:sup> MgCl<jats:sub>2</jats:sub>, reaction at 70 °C for 2.9 h, resulting in a maximum Mg content of 2.13%. Next, the physicochemical properties and structural characteristics of ALP1 and ALP1‐Mg are characterized, and the results show that the morphology, conformation, crystallinity, and thermal stability of ALP1‐Mg are changed. In addition, dietary supplementation of 500 mg kg<jats:sup>−1</jats:sup> ALP1‐Mg significantly reduces the feed conversion ratio during the grower (15–35 d). Meanwhile, the villus height/crypt depth of the duodenum and ileum are significantly increased, and the relative abundance of <jats:italic>Lactobacillus</jats:italic> is significantly elevated. Taken together, the results suggest that ALP1‐Mg is a potential growth‐promoting feed additive.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phytotherapeutic Hierarchical PCL‐Based Scaffolds as a Multifunctional Wound Dressing: Combining 3D Printing and Electrospinning 以植物疗法为基础的分层 PCL 支架作为多功能伤口敷料:三维打印与电纺丝的结合
IF 4.6 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-10 DOI: 10.1002/mabi.202400253
Irem Unalan, Benedikt Slavik, Andrea Buettner, Aldo R. Boccaccini
This study focuses on developing hybrid scaffolds incorporating phytotherapeutic agents via a combination of three‐dimensional (3D) printing and electrospinning to enhance mechanical properties and provide antibacterial activity, in order to address the limitations of traditional antibiotics. In this regard, 3D‐printed polycaprolactone (PCL) struts are first fabricated using fused deposition modeling (FDM). Then, alkaline surface treatment is applied to improve the adhesion of electrospun nanofibers. Finally, peppermint oil (PEP) or clove oil (CLV)‐incorporated PCL‐gelatin (GEL) electrospun nanofibers are collected on top of the 3D‐printed PCL scaffolds by electrospinning. Incorporating PEP or CLV into PCL‐GEL electrospun nanofibers enhances the scaffold's layer detachment and adhesion force. In addition, the DPPH free radical scavenging activity assay indicates that incorporating PEP or CLV improves the antioxidant properties of the scaffolds. Further, antibacterial activity results reveal that PEP or CLV incorporated scaffolds exhibit inhibition against Staphylococcus aureus and Escherichia coli bacteria. Moreover, anti‐inflammatory assays show that scaffolds reduce the concentration of nitric oxide (NO) released from Raw 264.7 macrophage‐like cells. On the other hand, the phytotherapeutic hierarchical scaffolds have no toxic effect on normal human dermal fibroblast (NHDF) cells, and PEP or CLV enhance cell attachment and proliferation. Overall, incorporating natural phytotherapeutic agents into hierarchical scaffolds shows promise for advancing wound healing applications.
本研究的重点是通过三维(3D)打印和电纺丝相结合的方法,开发含有植物治疗剂的混合支架,以增强其机械性能并提供抗菌活性,从而解决传统抗生素的局限性。为此,首先使用熔融沉积建模技术(FDM)制造三维打印聚己内酯(PCL)支柱。然后进行碱性表面处理,以提高电纺纳米纤维的附着力。最后,通过电纺丝将薄荷油(PEP)或丁香油(CLV)融入 PCL-明胶(GEL)电纺纳米纤维收集到 3D 打印的 PCL 支架上。在 PCL-GEL 电纺纳米纤维中加入 PEP 或 CLV 可增强支架层的剥离力和粘附力。此外,DPPH 自由基清除活性检测表明,加入 PEP 或 CLV 可提高支架的抗氧化性。此外,抗菌活性结果表明,加入 PEP 或 CLV 的支架对金黄色葡萄球菌和大肠杆菌有抑制作用。此外,抗炎试验表明,支架降低了 Raw 264.7 巨噬细胞释放的一氧化氮(NO)浓度。另一方面,植物疗法分层支架对正常人真皮成纤维细胞(NHDF)没有毒性作用,而 PEP 或 CLV 能增强细胞附着和增殖。总之,在分层支架中加入天然植物治疗剂有望促进伤口愈合应用。
{"title":"Phytotherapeutic Hierarchical PCL‐Based Scaffolds as a Multifunctional Wound Dressing: Combining 3D Printing and Electrospinning","authors":"Irem Unalan, Benedikt Slavik, Andrea Buettner, Aldo R. Boccaccini","doi":"10.1002/mabi.202400253","DOIUrl":"https://doi.org/10.1002/mabi.202400253","url":null,"abstract":"This study focuses on developing hybrid scaffolds incorporating phytotherapeutic agents via a combination of three‐dimensional (3D) printing and electrospinning to enhance mechanical properties and provide antibacterial activity, in order to address the limitations of traditional antibiotics. In this regard, 3D‐printed polycaprolactone (PCL) struts are first fabricated using fused deposition modeling (FDM). Then, alkaline surface treatment is applied to improve the adhesion of electrospun nanofibers. Finally, peppermint oil (PEP) or clove oil (CLV)‐incorporated PCL‐gelatin (GEL) electrospun nanofibers are collected on top of the 3D‐printed PCL scaffolds by electrospinning. Incorporating PEP or CLV into PCL‐GEL electrospun nanofibers enhances the scaffold's layer detachment and adhesion force. In addition, the DPPH free radical scavenging activity assay indicates that incorporating PEP or CLV improves the antioxidant properties of the scaffolds. Further, antibacterial activity results reveal that PEP or CLV incorporated scaffolds exhibit inhibition against <jats:italic>Staphylococcus aureus</jats:italic> and <jats:italic>Escherichia coli</jats:italic> bacteria. Moreover, anti‐inflammatory assays show that scaffolds reduce the concentration of nitric oxide (NO) released from Raw 264.7 macrophage‐like cells. On the other hand, the phytotherapeutic hierarchical scaffolds have no toxic effect on normal human dermal fibroblast (NHDF) cells, and PEP or CLV enhance cell attachment and proliferation. Overall, incorporating natural phytotherapeutic agents into hierarchical scaffolds shows promise for advancing wound healing applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metals at the Helm: Revolutionizing Protein Assembly and Applications. 金属掌舵:彻底改变蛋白质组装和应用。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-06 DOI: 10.1002/mabi.202400126
Maoping Duan, Chenyan Lv, Jiachen Zang, Xiaojing Leng, Guanghua Zhao, Tuo Zhang

Protein assembly is an essential process in biological systems, where proteins self-assemble into complex structures with diverse functions. Inspired by the exquisite control over protein assembly in nature, scientists have been exploring ways to design and assemble protein structures with precise control over their topologies and functions. One promising approach for achieving this goal is through metal coordination, which utilizes metal-binding motifs to mediate protein-protein interactions and assemble protein complexes with controlled stoichiometry and geometry. Metal coordination provides a modular and tunable approach for protein assembly and de novo structure design, where the metal ion acts as a molecular glue that holds the protein subunits together in a specific orientation. Metal-coordinated protein assemblies have shown great potential for developing functional metalloproteinase, novel biomaterials and integrated drug delivery systems. In this review, an overview of the recent advances in protein assemblies benefited from metal coordination is provided, focusing on various protein arrangements in different dimensions including protein oligomers, protein nanocage and higher-order protein architectures. Moreover, the key metal-binding motifs and strategies used to assemble protein structures with precise control over their properties are highlighted. The potential applications of metal-mediated protein assemblies in biotechnology and biomedicine are also discussed.

蛋白质组装是生物系统中的一个基本过程,在这个过程中,蛋白质会自我组装成具有不同功能的复杂结构。受到自然界对蛋白质组装的精妙控制的启发,科学家们一直在探索如何设计和组装蛋白质结构,并精确控制其拓扑结构和功能。金属配位是实现这一目标的一种很有前景的方法,它利用金属结合基团来介导蛋白质与蛋白质之间的相互作用,并组装出具有可控化学计量和几何形状的蛋白质复合物。金属配位为蛋白质组装和新结构设计提供了一种模块化和可调整的方法,其中金属离子就像一种分子胶水,将蛋白质亚基按特定方向粘合在一起。金属配位蛋白质组装在开发功能性金属蛋白酶、新型生物材料和集成药物输送系统方面具有巨大潜力。本综述概述了得益于金属配位的蛋白质组装的最新进展,重点关注不同维度的各种蛋白质排列,包括蛋白质寡聚体、蛋白质纳米笼和高阶蛋白质结构。此外,还重点介绍了用于组装蛋白质结构并精确控制其特性的关键金属结合基团和策略。还讨论了金属介导的蛋白质组装在生物技术和生物医学中的潜在应用。
{"title":"Metals at the Helm: Revolutionizing Protein Assembly and Applications.","authors":"Maoping Duan, Chenyan Lv, Jiachen Zang, Xiaojing Leng, Guanghua Zhao, Tuo Zhang","doi":"10.1002/mabi.202400126","DOIUrl":"https://doi.org/10.1002/mabi.202400126","url":null,"abstract":"<p><p>Protein assembly is an essential process in biological systems, where proteins self-assemble into complex structures with diverse functions. Inspired by the exquisite control over protein assembly in nature, scientists have been exploring ways to design and assemble protein structures with precise control over their topologies and functions. One promising approach for achieving this goal is through metal coordination, which utilizes metal-binding motifs to mediate protein-protein interactions and assemble protein complexes with controlled stoichiometry and geometry. Metal coordination provides a modular and tunable approach for protein assembly and de novo structure design, where the metal ion acts as a molecular glue that holds the protein subunits together in a specific orientation. Metal-coordinated protein assemblies have shown great potential for developing functional metalloproteinase, novel biomaterials and integrated drug delivery systems. In this review, an overview of the recent advances in protein assemblies benefited from metal coordination is provided, focusing on various protein arrangements in different dimensions including protein oligomers, protein nanocage and higher-order protein architectures. Moreover, the key metal-binding motifs and strategies used to assemble protein structures with precise control over their properties are highlighted. The potential applications of metal-mediated protein assemblies in biotechnology and biomedicine are also discussed.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone-Mimetic Osteon Microtopographies on Poly-ε-Caprolactone Enhance the Osteogenic Potential of Human Mesenchymal Stem Cells. 聚ε-己内酯上的仿骨骨微结构可增强人间质干细胞的成骨潜能
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-05 DOI: 10.1002/mabi.202400311
Matthias Vostatek, Elettra Verin, Marvin Tamm, Mario Rothbauer, Stefan Toegel, Francesco Moscato

The attributes of implant surfaces are pivotal for successful osseointegration. Among surface engineering strategies, microtopography stands out as a promising approach to promote early cellular interactions. This study aims to design and craft a novel biomimetic osteon-like surface modification and to compare its impact on human mesenchymal stem cells (hMSCs) with four established topographies: blank, inverted pyramids, protrusions, and grooves. Poly-ε-caprolactone samples are fabricated using 2-photon-polymerization and soft lithography, prior to analysis via scanning electron microscopy (SEM), water contact angle (WCA), and protein adsorption assays. Additionally, cellular responses including cell attachment, proliferation, morphology, cytoskeletal organization, and osteogenic differentiation potential are evaluated. SEM confirms the successful fabrication of microtopographies, with minimal effect on WCA and protein adsorption. Cell attachment experiments demonstrate a significant increase on the osteon-like structure, being three times higher than on the blank. Proliferation assays indicate a fourfold increase with osteon-like microtopography compared to the blank, while ALP activity is notably elevated with osteon-like microtopography at days 7 (threefold increase over blank) and 14 (fivefold increase over blank). In conclusion, the novel biomimetic osteon-like structure demonstrates favorable responses from hMSCs, suggesting potential for promoting successful implant integration in vivo.

种植体表面的特性是成功骨结合的关键。在表面工程策略中,微表层设计是促进早期细胞相互作用的一种有前途的方法。本研究旨在设计和制作一种新型仿生骨样表面改性材料,并比较其与四种已有形貌(空白、倒金字塔、突起和凹槽)对人类间充质干细胞(hMSCs)的影响。在通过扫描电子显微镜(SEM)、水接触角(WCA)和蛋白质吸附试验进行分析之前,使用双光子聚合和软光刻技术制作了聚-ε-己内酯样品。此外,还评估了细胞反应,包括细胞附着、增殖、形态、细胞骨架组织和成骨分化潜力。扫描电子显微镜(SEM)证实成功制造了微形貌,对 WCA 和蛋白质吸附的影响极小。细胞附着实验表明,骨样结构的细胞附着率明显增加,是空白结构的三倍。增殖实验表明,骨样微拓扑结构上的细胞比空白结构上的细胞增殖了四倍,而骨样微拓扑结构上的 ALP 活性在第 7 天(比空白结构上的细胞增殖了三倍)和第 14 天(比空白结构上的细胞增殖了五倍)明显升高。总之,新型仿生骨样结构显示了 hMSCs 的良好反应,表明它具有促进体内植入物成功整合的潜力。
{"title":"Bone-Mimetic Osteon Microtopographies on Poly-ε-Caprolactone Enhance the Osteogenic Potential of Human Mesenchymal Stem Cells.","authors":"Matthias Vostatek, Elettra Verin, Marvin Tamm, Mario Rothbauer, Stefan Toegel, Francesco Moscato","doi":"10.1002/mabi.202400311","DOIUrl":"https://doi.org/10.1002/mabi.202400311","url":null,"abstract":"<p><p>The attributes of implant surfaces are pivotal for successful osseointegration. Among surface engineering strategies, microtopography stands out as a promising approach to promote early cellular interactions. This study aims to design and craft a novel biomimetic osteon-like surface modification and to compare its impact on human mesenchymal stem cells (hMSCs) with four established topographies: blank, inverted pyramids, protrusions, and grooves. Poly-ε-caprolactone samples are fabricated using 2-photon-polymerization and soft lithography, prior to analysis via scanning electron microscopy (SEM), water contact angle (WCA), and protein adsorption assays. Additionally, cellular responses including cell attachment, proliferation, morphology, cytoskeletal organization, and osteogenic differentiation potential are evaluated. SEM confirms the successful fabrication of microtopographies, with minimal effect on WCA and protein adsorption. Cell attachment experiments demonstrate a significant increase on the osteon-like structure, being three times higher than on the blank. Proliferation assays indicate a fourfold increase with osteon-like microtopography compared to the blank, while ALP activity is notably elevated with osteon-like microtopography at days 7 (threefold increase over blank) and 14 (fivefold increase over blank). In conclusion, the novel biomimetic osteon-like structure demonstrates favorable responses from hMSCs, suggesting potential for promoting successful implant integration in vivo.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alginate Capsules: Versatile Applications and Production Techniques. 藻酸盐胶囊:多种应用和生产技术。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-05 DOI: 10.1002/mabi.202400202
Francesca Passannanti, Marianna Gallo, Giulia Lentini, Rosa Colucci Cante, Federica Nigro, Roberto Nigro, Andrea Budelli

Alginate is a natural polysaccharide commonly obtained from brown algae and is usually used in the food industry as an additive, specifically as a thickening, gelling, and emulsifying agent. Due to its polyanionic nature, it can crosslink in the presence of divalent or trivalent cations. This crosslinking process involves the formation of chemical bonds between the carboxylic groups of parallel chains, resulting in a solid structure. In this way, compounds of interest can be enclosed in a capsule or a bead. Thanks to this ability, possible applications of alginate capsules are countless: it is possible to range from the pharmaceutical to the nutritional fields, from the agri-food industry to the textile or cosmetic sectors. These capsules can protect the encapsulated ingredients, promote their delivery or controlled release, or be imagined as small-scale reactors. The present review describes the main techniques used to produce alginate capsules, and several examples of possible application fields are shown.

藻酸盐是一种天然多糖,通常从褐藻中提取,在食品工业中通常用作添加剂,特别是增稠剂、胶凝剂和乳化剂。由于其多阴离子性质,它可以在二价或三价阳离子存在下发生交联。这种交联过程包括在平行链的羧基之间形成化学键,从而形成固体结构。这样,相关化合物就可以被包裹在胶囊或珠子中。得益于这种能力,海藻酸盐胶囊的应用领域数不胜数:从制药到营养领域,从农业食品工业到纺织或化妆品行业,无所不包。这些胶囊可以保护封装的成分,促进其输送或控制释放,也可以想象成小型反应器。本综述介绍了用于生产海藻酸盐胶囊的主要技术,并举例说明了可能的应用领域。
{"title":"Alginate Capsules: Versatile Applications and Production Techniques.","authors":"Francesca Passannanti, Marianna Gallo, Giulia Lentini, Rosa Colucci Cante, Federica Nigro, Roberto Nigro, Andrea Budelli","doi":"10.1002/mabi.202400202","DOIUrl":"https://doi.org/10.1002/mabi.202400202","url":null,"abstract":"<p><p>Alginate is a natural polysaccharide commonly obtained from brown algae and is usually used in the food industry as an additive, specifically as a thickening, gelling, and emulsifying agent. Due to its polyanionic nature, it can crosslink in the presence of divalent or trivalent cations. This crosslinking process involves the formation of chemical bonds between the carboxylic groups of parallel chains, resulting in a solid structure. In this way, compounds of interest can be enclosed in a capsule or a bead. Thanks to this ability, possible applications of alginate capsules are countless: it is possible to range from the pharmaceutical to the nutritional fields, from the agri-food industry to the textile or cosmetic sectors. These capsules can protect the encapsulated ingredients, promote their delivery or controlled release, or be imagined as small-scale reactors. The present review describes the main techniques used to produce alginate capsules, and several examples of possible application fields are shown.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Macromolecular bioscience
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1