Yee, T. L., Azuddin, N. F., Mohd, M. H., Zakaria, L.
{"title":"Occurrence and identification of Penicillium and Talaromyces species from beach sand","authors":"Yee, T. L., Azuddin, N. F., Mohd, M. H., Zakaria, L.","doi":"10.21161/mjm.220005","DOIUrl":"https://doi.org/10.21161/mjm.220005","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"32 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76460412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wan-Nor-Amilah, W. A. W., Lai, J. H., Musa, I., Sul’ain, M. D.
{"title":"In vitro anti-Candida activity of Melaleuca cajuputi extracts","authors":"Wan-Nor-Amilah, W. A. W., Lai, J. H., Musa, I., Sul’ain, M. D.","doi":"10.21161/mjm.221510","DOIUrl":"https://doi.org/10.21161/mjm.221510","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"12 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74408436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: Endophytic fungi are a diverse group of microorganisms that stay asymptomatically in the healthy tissues of the host. Many fungal endophytes are associated with the tea plant ( Camellia sinensis ) and the pathogens of the tea plant have the potential to grow as endophytes or act as latent pathogens during the initial growth of the plant. The present study aimed at screening tea endophytic fungi with the potential for control of a few critical phytopathogens, Fusarium sp., Lasiodiplodia theobromae , Pestalotiopsis sp. and Sclerotinia sclerotiorum while evaluating the efficiency of growth inhibition of these phytopathogens by the endophytic fungal isolates using in vitro assays. Methodology and results: Five endophytic fungal strains; Colletotrichum gloeosporioides , C. siamense , Daldinia eschscholtzii , Pseudopestalotiopsis chinensis and Phyllosticta capitalensis isolated from leaves of C. sinensis in Sri Lanka were evaluated for growth inhibition against plant pathogens; Fusarium sp., L. theobromae , Pestalotiopsis sp. and S . sclerotiorum using the dual culture assay and volatile compound-mediated inhibition assay. All the fungal endophytes used in this study exhibited antifungal activity against Fusarium sp., Pestalotiopsis sp. and S . sclerotiorum in the dual culture assay on PDA. Daldinia eschscholtzii (67.30%) and C. gloeosporioides (61.54%) showed strong antagonistic activity against S. sclerotiorum , while P. capitalensis (42.30%) demonstrated moderate activity. All the endophytic fungal strains showed moderate antifungal activities against Fusarium sp. The percentage growth inhibitions of Pestalotiopsis sp. by all the endophytic isolates tested were below 25.00%. In the volatile compound-mediated inhibition assay, none of the endophytic isolates showed visible inhibition against the phytopathogens used. Conclusion, significance and impact of study: In this study, the fungal endophytes which showed potential antagonistic activity against the tested phytopathogens can be used to develop commercialized products of fungal biocontrol agents (BCAs) for controlling plant fungal diseases.
{"title":"Biocontrol potential of endophytic fungi in tea (Camellia sinensis (L.) Kuntze) leaves against selected fungal phytopathogens","authors":"Kasun M. Thambugala, D. Daranagama, S. Kannangara","doi":"10.21161/mjm.220055","DOIUrl":"https://doi.org/10.21161/mjm.220055","url":null,"abstract":"Aims: Endophytic fungi are a diverse group of microorganisms that stay asymptomatically in the healthy tissues of the host. Many fungal endophytes are associated with the tea plant ( Camellia sinensis ) and the pathogens of the tea plant have the potential to grow as endophytes or act as latent pathogens during the initial growth of the plant. The present study aimed at screening tea endophytic fungi with the potential for control of a few critical phytopathogens, Fusarium sp., Lasiodiplodia theobromae , Pestalotiopsis sp. and Sclerotinia sclerotiorum while evaluating the efficiency of growth inhibition of these phytopathogens by the endophytic fungal isolates using in vitro assays. Methodology and results: Five endophytic fungal strains; Colletotrichum gloeosporioides , C. siamense , Daldinia eschscholtzii , Pseudopestalotiopsis chinensis and Phyllosticta capitalensis isolated from leaves of C. sinensis in Sri Lanka were evaluated for growth inhibition against plant pathogens; Fusarium sp., L. theobromae , Pestalotiopsis sp. and S . sclerotiorum using the dual culture assay and volatile compound-mediated inhibition assay. All the fungal endophytes used in this study exhibited antifungal activity against Fusarium sp., Pestalotiopsis sp. and S . sclerotiorum in the dual culture assay on PDA. Daldinia eschscholtzii (67.30%) and C. gloeosporioides (61.54%) showed strong antagonistic activity against S. sclerotiorum , while P. capitalensis (42.30%) demonstrated moderate activity. All the endophytic fungal strains showed moderate antifungal activities against Fusarium sp. The percentage growth inhibitions of Pestalotiopsis sp. by all the endophytic isolates tested were below 25.00%. In the volatile compound-mediated inhibition assay, none of the endophytic isolates showed visible inhibition against the phytopathogens used. Conclusion, significance and impact of study: In this study, the fungal endophytes which showed potential antagonistic activity against the tested phytopathogens can be used to develop commercialized products of fungal biocontrol agents (BCAs) for controlling plant fungal diseases.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"30 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86605948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Al-Tameemi, K., Nassour, R., Sleman, R. A., Daoud, M. S., Murad, L. S.
Aims: Coronavirus disease-2019 (COVID-19) is an ongoing global pandemic. In Syria, the first case was reported in March 2020. This study was aimed to investigate the incidences of COVID-19 in the Banias and Alkadmos areas in Tartus, Syria, in 2021.Methodology and results: Our data were obtained from the records of Banias and Alkadmos hospitals. The results were inferred by analysing data for 1026 COVID-19 patients. About 58.4% of total cases were among males and 41.6% were among females. Most patients were living in rural areas;73% compared to 27% in urban ones. The severity of the disease varied between patients. About 67.4% of cases needed to be hospitalized for treatment, most of which were from the elderly. The same was noticed with the mortality rate, with 14.5% of total cases and 66.4% of them were older than 65.Conclusion, significance and impact of study: Our study indicated that age and gender are risk factors for contracting COVID-19.
{"title":"A study on the epidemiology of COVID-19 in the Banias and Alkadmos areas of Syria during 2021.","authors":"Al-Tameemi, K., Nassour, R., Sleman, R. A., Daoud, M. S., Murad, L. S.","doi":"10.21161/mjm.221505","DOIUrl":"https://doi.org/10.21161/mjm.221505","url":null,"abstract":"Aims: Coronavirus disease-2019 (COVID-19) is an ongoing global pandemic. In Syria, the first case was reported in March 2020. This study was aimed to investigate the incidences of COVID-19 in the Banias and Alkadmos areas in Tartus, Syria, in 2021.Methodology and results: Our data were obtained from the records of Banias and Alkadmos hospitals. The results were inferred by analysing data for 1026 COVID-19 patients. About 58.4% of total cases were among males and 41.6% were among females. Most patients were living in rural areas;73% compared to 27% in urban ones. The severity of the disease varied between patients. About 67.4% of cases needed to be hospitalized for treatment, most of which were from the elderly. The same was noticed with the mortality rate, with 14.5% of total cases and 66.4% of them were older than 65.Conclusion, significance and impact of study: Our study indicated that age and gender are risk factors for contracting COVID-19.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"49 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87010113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayman D. I. Alsheikh, Hana M. Sawan, Shatha M. S. Al Omari, Shorouq M. M. Asad
Aims: Due to the growing number of media reports claiming that books contain germs, it is crucial to look into the possibility that contagious diseases could spread through libraries. The aim of the study was to identify bacteria from various fomites in four Jordanian university libraries and to assess the antibacterial resistance pattern of isolates. Methodology and results: In this study, swab samples were taken from different fomites of four Jordanian university libraries. Samples were then cultivated on nutrient agar and incubated aerobically at 37 °C for 48 h. To identify different types of isolated bacteria, biochemical and conventional biochemical tests were applied using the qualitative RapID TM One System with the help of ERICTM software to identify the bacterial isolates at the species level. Identified bacterial species, including Escherichia coli , Shigella sonnei , Pseudomonas aeruginosa , Klebsiella pneumonia , Staphylococcus epidermis , S. aureus , Salmonella choleraesuis , Bacillus subtilis and Citrobacter freundii were isolated from different library fomites. Seventy-one bacterial isolates from University A were observed to be multidrug-resistant (MDR) ( S. sonnei and S. choleraesuis ) . This MDR pattern is alarming as those isolates were found in a public environment and that imposes a direct threat on library users, staff and visitors . Conclusion, significance and impact of study: University libraries' fomites carry live bacterial pathogens, which can contaminate users' hands and serve as an indirect route for spreading antibiotic resistance and microbial illnesses. While more research is required, considering hand hygiene improvement would be the simplest infection control technique at libraries. Additionally, proactive measures should be taken to track the prevalence of harmful microorganisms in these settings and their effects on employees' and the public's health.
{"title":"Antibiotic susceptibility of bacteria recovered from university libraries in Jordan","authors":"Ayman D. I. Alsheikh, Hana M. Sawan, Shatha M. S. Al Omari, Shorouq M. M. Asad","doi":"10.21161/mjm.221515","DOIUrl":"https://doi.org/10.21161/mjm.221515","url":null,"abstract":"Aims: Due to the growing number of media reports claiming that books contain germs, it is crucial to look into the possibility that contagious diseases could spread through libraries. The aim of the study was to identify bacteria from various fomites in four Jordanian university libraries and to assess the antibacterial resistance pattern of isolates. Methodology and results: In this study, swab samples were taken from different fomites of four Jordanian university libraries. Samples were then cultivated on nutrient agar and incubated aerobically at 37 °C for 48 h. To identify different types of isolated bacteria, biochemical and conventional biochemical tests were applied using the qualitative RapID TM One System with the help of ERICTM software to identify the bacterial isolates at the species level. Identified bacterial species, including Escherichia coli , Shigella sonnei , Pseudomonas aeruginosa , Klebsiella pneumonia , Staphylococcus epidermis , S. aureus , Salmonella choleraesuis , Bacillus subtilis and Citrobacter freundii were isolated from different library fomites. Seventy-one bacterial isolates from University A were observed to be multidrug-resistant (MDR) ( S. sonnei and S. choleraesuis ) . This MDR pattern is alarming as those isolates were found in a public environment and that imposes a direct threat on library users, staff and visitors . Conclusion, significance and impact of study: University libraries' fomites carry live bacterial pathogens, which can contaminate users' hands and serve as an indirect route for spreading antibiotic resistance and microbial illnesses. While more research is required, considering hand hygiene improvement would be the simplest infection control technique at libraries. Additionally, proactive measures should be taken to track the prevalence of harmful microorganisms in these settings and their effects on employees' and the public's health.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"163 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83212205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kamal, N. M., Shariff, F. M., Normi, Y. M., Salleh, A. B., Oslan, S. N.
{"title":"Analysis of a thermostable lipase from CTG-clade yeast: Molecular expression, characterization and structure prediction","authors":"Kamal, N. M., Shariff, F. M., Normi, Y. M., Salleh, A. B., Oslan, S. N.","doi":"10.21161/mjm.221412","DOIUrl":"https://doi.org/10.21161/mjm.221412","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"37 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77963831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antibacterial activity of Bombyx mori and Samia cynthia ricini sericin proteins against Escherichia coli and Staphylococcus aureus","authors":"Aprilia, L., Endrawati, Y. C., Arief, I. I.","doi":"10.21161/mjm.221512","DOIUrl":"https://doi.org/10.21161/mjm.221512","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"164 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73135215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosli, N. A. S., Mohd Razali, R., Ahmad, F. T., Lob, S., M. Sidique, S. N., Siva, R.
{"title":"In vitro antifungal properties of Aloe vera (L.) Burm. f. films incorporated with cinnamon essential oil against Lasiodoplodia theobromae in wax apple","authors":"Rosli, N. A. S., Mohd Razali, R., Ahmad, F. T., Lob, S., M. Sidique, S. N., Siva, R.","doi":"10.21161/mjm.221401","DOIUrl":"https://doi.org/10.21161/mjm.221401","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"39 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90201584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mughis, Z., Ali, M. A., Anjum, A. A., Omer, M. O., Abbas, M., Najeeb, M.
{"title":"Molecular characterization of mycotoxin-producing Aspergillus parasiticus and sensitivity pattern to different disinfectants","authors":"Mughis, Z., Ali, M. A., Anjum, A. A., Omer, M. O., Abbas, M., Najeeb, M.","doi":"10.21161/mjm.221496","DOIUrl":"https://doi.org/10.21161/mjm.221496","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88971543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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